Descriptor: "Aeromonadaceae" - Searchworks@Jio Institute Digital Library Search Results

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425 results on '"Aeromonadaceae"'

101. Data on Aeromonas hydrophila Discussed by Researchers at Henan Normal University [Characterization of Hepcidin Gene and Protection of Recombinant Hepcidin Supplemented In Feed Against Aeromonas Hydrophila Infection In Yellow River Carp (Cyprinus...].

Published: 2023

102. Reports from Jilin Agricultural University Add New Data to Findings in Aeromonas [Ompr (Tcs Response Regulator) of Aeromonas Veronii Plays a Major Role In Drug Resistance, Stress Resistance and Virulence By Regulating Biofilm Formation].

Abstract: Keywords: Jilin; People's Republic of China; Asia; Aeromonadaceae; Aeromonas; Drug Resistance; Drugs and Therapies; Gammaproteobacteria; Gram-Negative Bacteria; Gram-Negative Facultatively Anaerobic Rods; Health and Medicine; Proteobacteria EN Jilin People's Republic of China Asia Aeromonadaceae Aeromonas Drug Resistance Drugs and Therapies Gammaproteobacteria Gram-Negative Bacteria Gram-Negative Facultatively Anaerobic Rods Health and Medicine Proteobacteria 1518 1518 1 08/07/23 20230808 NES 230808 2023 AUG 11 (NewsRx) -- By a News Reporter-Staff News Editor at Drug Week -- Current study results on Gram-Negative Bacteria - Aeromonas have been published. Jilin, People's Republic of China, Asia, Aeromonas, Drug Resistance, Drugs and Therapies, Gammaproteobacteria, Gram-Negative Bacteria, Gram-Negative Facultatively Anaerobic Rods, Health and Medicine, Proteobacteria, Aeromonadaceae. [Extracted from the article]
Published: 2023

103. Inhibition of Helicobacter pylori and Its Associated Urease by Palmatine: Investigation on the Potential Mechanism.

Author: Zhou, Jiang-Tao, Li, Cai-Lan, Tan, Li-Hua, Xu, Yi-Fei, Liu, Yu-Hong, Mo, Zhi-Zhun, Dou, Yao-Xing, Su, Rui, Su, Zi-Ren, Huang, Ping, and Xie, Jian-Hui
Subjects: *HELPLESSNESS (Psychology), *HELICOBACTER pylori, *GRAM-negative bacteria, *HELICOBACTER, *AEROMONADACEAE
Abstract: In this paper, we evaluated the anti-Helicobacter pylori activity and the possible inhibitory effect on its associated urease by Palmatine (Pal) from Coptis chinensis, and explored the potential underlying mechanism. Results indicated that Pal exerted inhibitory effect on four tested H. pylori strains (ATCC 43504, NCTC 26695, SS1 and ICDC 111001) by the agar dilution test with minimum inhibitory concentration (MIC) values ranging from 100 to 200 μg/mL under neutral environment (pH 7.4), and from 75 to 100 μg/mL under acidic conditions (pH 5.3), respectively. Pal was observed to significantly inhibit both H. pylori urease (HPU) and jack bean urease (JBU) in a dose-dependent manner, with IC50 values of 0.53 ± 0.01 mM and 0.03 ± 0.00 mM, respectively, as compared with acetohydroxamic acid, a well-known urease inhibitor (0.07 ± 0.01 mM for HPU and 0.02 ± 0.00 mM for JBU, respectively). Kinetic analyses showed that the type of urease inhibition by Pal was noncompetitive for both HPU and JBU. Higher effectiveness of thiol protectors against urease inhibition than the competitive Ni2+ binding inhibitors was observed, indicating the essential role of the active-site sulfhydryl group in the urease inhibition by Pal. DTT reactivation assay indicated that the inhibition on the two ureases was reversible, further supporting that sulfhydryl group should be obligatory for urease inhibition by Pal. Furthermore, molecular docking study indicated that Pal interacted with the important sulfhydryl groups and inhibited the active enzymatic conformation through N-H ∙ π interaction, but did not interact with the active site Ni2+. Taken together, Pal was an effective inhibitor of H. pylori and its urease targeting the sulfhydryl groups, representing a promising candidate as novel urease inhibitor. This investigation also gave additional scientific support to the use of C. chinensis to treat H. pylori-related gastrointestinal diseases in traditional Chinese medicine. Pal might be a potentially beneficial therapy for gastritis and peptic ulcers induced by H. pylori infection and other urease-related diseases. [ABSTRACT FROM AUTHOR]
Published: 2017
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104. Drug resistance, AmpC-β-lactamase and extended-spectrum β-lactamase-producing Enterobacteriaceae isolated from fish and shrimp.

Author: de Almeida, Marília Viana Albuquerque, Cangussú, Ítalo Mendes, de Carvalho, Antonia Leonadia Siqueira, Ponte Brito, Izabelly Linhares, and Costa, Renata Albuquerque
Subjects: DRUG resistance, PHARMACOLOGY, GRAM-negative bacteria, AEROMONADACEAE, ENTEROBACTERIACEAE
Abstract: the present study aims to detect the production of extended-spectrum beta-lactamases (ESBL) by enterobacteria isolated from samples of fresh shrimp and fish obtained from the retail trade of the city of Sobral, Ceará State, Brazil. All bacterial isolates were submitted to identification and antimicrobial susceptibility testing using aminopenicillin, beta-lactamase inhibitors, carbapenem, 1st, 2nd, 3rd and 4th generation cephalosporins, and monobactam. three types of beta-lactamases -ESBL, AmpC and KPC -were investigated. 103 strains were identified, and the most frequent species in shrimp and fish samples was Enterobacter cloacae (n = 54). All the strains were resistant to penicillin and more than 50% of the isolates were resistant to ampicillin and cephalothin. Resistance to three 3rd generation cephalosporins (cefotaxime, ceftriaxone and ceftazidime) and one fourth generation cephalosporin (cefepime) was detected in two isolates of E. cloacae from shrimp samples. Phenotypic detection of AmpC was confirmed in seven strains.
Published: 2017
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105. Molecular Dynamics Simulations Predict the Pathways via Which Pristine Fullerenes Penetrate Bacterial Membranes.

Author: Pin-Chia Hsu, Jefferies, Damien, and Khalid, Syma
Subjects: *MOLECULAR structure of fullerenes, *MOLECULAR structure of microclusters, *GRAM-negative bacteria, *AEROMONADACEAE, *PALMITOYLATION
Abstract: Carbon fullerenes are emerging as effective devices for different biomedical applications, including the transportation of nanosized drugs and extraction of harmful oxidants and radicals. It has been proposed that fullerenes could be used as novel antibacterial agents, given the realization that the nanoparticles can kill pathogenic Gram-negative bacteria. To explore this at the molecular level, we simulated C60 fullerenes with bacterial membranes using the coarse-grain molecular dynamics Martini force field. We found that pristine C60 has a limited tendency to penetrate (incomplete core) Re mutant lipopolysaccharide (LPS) leaflets, but the translocation of C60 fullerenes into (complete core) Ra mutant LPS leaflets is not thermodynamically favored. Moreover, we showed that the permeability of the Re LPS bilayers depends sensitively on the system temperature, charge of ambient ions, and prevalence of palmitoyloleoylphosphoethanolamine (POPE) defect domains. The different permeabilities are rationalized in terms of transitory head group pore formation, which underpins the translocation of C60 into the lipid core. The Re LPS lipids readily form transient micropores when they are linked with monovalent cations or when they are heated to a high temperature. POPE lipids are shown to be particularly adept at forming these transient surface cavities, and their inclusion into Re LPS membranes facilitates the formation of particularly large pores that are tunneled by C60 aggregates of a significant size (~5 nm wide). After insertion into the lipid core, the aggregates dissociate, and the disbanded nanoparticles migrate to the interface between separate POPE and LPS domains, where they weaken the boundaries between the coexisting lipid fractions and thereby promote lipid mixing. [ABSTRACT FROM AUTHOR]
Published: 2016
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106. An evaluation of fish health-management practices and occupational health hazards associated with Pangasius catfish ( Pangasianodon hypophthalmus) aquaculture in the Mekong Delta, Vietnam.

Author: Phu, Tran Minh, Phuong, Nguyen Thanh, Dung, Tu Thanh, Hai, Dao Minh, Son, Vo Nam, Rico, Andreu, Clausen, Jesper Hedegaard, Madsen, Henry, Murray, Francis, and Dalsgaard, Anders
Subjects: *ANIMAL nutrition, *PROBIOTICS, *FISH breeding, *OCCUPATIONAL hazards, *PANGASIUS, *AQUACULTURE, *AEROMONADACEAE
Abstract: This study aimed to evaluate the current status on the use of probiotics, disinfectants and antimicrobials in hatcheries, nurseries and grow-out farms producing Pangasius catfish ( Pangasianodon hypophthalmus) in the Mekong Delta, Vietnam. Semi-structured interviews were conducted with 83 aquaculture enterprises (15 hatcheries, 32 nurseries and 36 grow-out farms). Farmers reported use of a total of 24 different antimicrobials, e.g. for treatment of bacillary necrosis and motile aeromonad septicaemia, and a variety of disinfectants, probiotics and nutritional supplements. In contrast to small-scale farmers, all large-scale grow-out farmers studied were certified and therefore had higher levels of formal education and specialized aquaculture training to diagnose and treat diseases. All farmers prepared their own medicated feed with a high risk of treatment failure, negative environmental impact from released antimicrobials and resistance development. Small-scale farmers were at particular occupational health risks when handling antimicrobials and other chemicals, e.g. mixing medicated feed with bare hands. There is an urgent need to improve knowledge and use innovative approaches, e.g. private-public partnerships, to assure a prudent use of chemicals, to improve capacity and access to disease diagnosis, particularly for small-scale grow-out farmers and nurseries. Efforts to control use of antimicrobials in aquaculture should be coordinated with the livestock and human health sectors taking an One-Health approach. [ABSTRACT FROM AUTHOR]
Published: 2016
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107. Isolation, Identification and in vitro Antimicrobial Susceptibility of Pathogenic Aeromorias veronii from Soft-shelled Turtles.

Author: Zhixiu ZHU, Xinhua JIANG, Shunzhou DENG, Bei WANG, Huihong LI, and Tianyue XU
Subjects: *AEROMONADACEAE, *ANTI-infective agents, *MICROBIAL sensitivity tests, *TURTLES, *BACILLUS amyloliquefaciens, *DISEASES
Abstract: In order to effectively control diseases caused by Aeromonas veronii, pathogenic bacteria were isolated arid incubated from infected soft-sheiled turtles with traditional bacterial isolation method. Four strains of pathogenic bacteria were isolated arid identified with tradition a 丨 biochemical identification method and modem molecular biological identification techniques. According to the results, four strains of pathogenic bacteria were identified as A. veronii biovar sobria. Drug sensitivity test and in vitro antimicrobial test against Bacillus amyloliquefaciens were performed with agar diffusion method. The results showed that B. amyloliquefaciens and several antibiotics such as of loxacin and geritamicin exerted strong antimicrobial effects on four isolates. B. amyloliquefa ciens could be used for the prevention and control of diseases caused by A. veronii in aquaculture. [ABSTRACT FROM AUTHOR]
Published: 2016

108. Synthesis, Surface and Antimicrobial Activities of Cationic Gemini Surfactants with Semi-Rigid Spacers.

Author: Zhu, Hai‐lin, Hu, Zhi‐yong, Ma, Xue‐mei, Wang, Jian‐long, and Cao, Duan‐lin
Subjects: *SURFACE active agents, *GRAM-negative bacteria, *AEROMONADACEAE, *THERMODYNAMICS, *MECHANICS (Physics)
Abstract: Four cationic gemini surfactants featuring semi-rigid spacers were synthesized via a two-step process. The surface-active properties of these surfactants were investigated through surface tension and electrical conductivity measurement. The thermodynamic parameters of micellization were evaluated from electrical conductivity measurements at temperatures ranging from 293 to 313 K. The aggregation behavior of these synthesized gemini surfactants in water were investigated using dynamic light scattering and transmission electron microscopy. Further, the antimicrobial activities of these synthesized gemini surfactants against both Gram-positive and Gram-negative bacteria were also investigated. [ABSTRACT FROM AUTHOR]
Published: 2016
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109. Helicobacter pylori colonization in Nepal; assessment of prevalence and potential risk factors in a hospital-based patient cohort.

Author: Ansari, Shamshul, Gautam, Rajendra, Nepal, Hari Prasad, Subedi, Shankar Nand, Shrestha, Sony, Mandal, Fuleshwar, Rimal, Brihaspati, and Chhetri, Muni Raj
Subjects: *HELICOBACTER pylori, *HELICOBACTER, *GRAM-negative bacteria, *BACTERIA, *AEROMONADACEAE
Abstract: Background: Helicobacter pylori, a gram-negative bacterium, can cause gastritis, peptic and duodenal ulcers. It is considered an important public health problem for both developed and developing world. This bacterium is classified as the class 1 carcinogen because it can cause cancer. Methods: A hospital based study was conducted at Chitwan Medical College Teaching Hospital (CMCTH) from May to October 2014. Stool samples were collected from the suspected patients and were subjected to detection of the H. pylori stool antigen (HpSAg) following the procedures recommended by the manufacturer. A standard questionnaire on the potential risk factors was also designed and completed. Result: HpSAg was detected in 16 % of suspected patients. The children up to 10 years of age were found to be highly infected (36 %). The patients living in urban area were found more susceptible to develop H. pylori infection (P < 0.05). Tea drinking and repeated eating habit (more than twice a day) were listed as the important factors that can limit the H. pylori infections significantly (P < 0.05). Conclusion: In this hospital based study, a significant rate of prevalence was evaluated. However, we recommend a community based extensive study to reveal the real scenario of H. pylori infection in Nepalese populations. [ABSTRACT FROM AUTHOR]
Published: 2016
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110. Assessing the Bacterial Community Composition of Bivalve Mollusks Collected in Aquaculture Farms and Respective Susceptibility to Antibiotics

Author: Eugénia Ferreira, Manuela Caniça, Vanessa Salgueiro, Maria João Botelho, Lígia Reis, and Vera Manageiro
Subjects: Microbiology (medical), Cefotaxime, antibiotic resistance, Antibiotic resistance, Oxytetracycline, Aquaculture, RM1-950, Biochemistry, Microbiology, Article, PMQR, Enterococcaceae, medicine, Aeromonadaceae, Pharmacology (medical), General Pharmacology, Toxicology and Pharmaceutics, Staphylococcaceae, bivalve mollusks, biology, business.industry, Sulfamethoxazole, biology.organism_classification, Raoultella ornithinolytica, Infectious Diseases, aquaculture, Bivalve mollusks, Flumequine, Therapeutics. Pharmacology, oxytetracycline, business, medicine.drug
Abstract: Aquaculture is a growing sector, providing several products for human consumption, and it is therefore important to guarantee its quality and safety. This study aimed to contribute to the knowledge of bacterial composition of Crassostrea gigas, Mytilus spp. and Ruditapes decussatus, and the antibiotic resistances/resistance genes present in aquaculture environments. Two hundred and twenty-two bacterial strains were recovered from all bivalve mollusks samples belonging to the Aeromonadaceae, Bacillaceae, Comamonadaceae, Enterobacteriaceae, Enterococcaceae, Micrococcaceae, Moraxellaceae, Morganellaceae, Pseudomonadaceae, Shewanellaceae, Staphylococcaceae, Streptococcaceae, Vibrionaceae, and Yersiniaceae families. Decreased susceptibility to oxytetracycline prevails in all bivalve species, aquaculture farms and seasons. Decreased susceptibilities to amoxicillin, amoxicillin/clavulanic acid, cefotaxime, cefoxitin, ceftazidime, chloramphenicol, florfenicol, colistin, ciprofloxacin, flumequine, nalidixic acid and trimethoprim/sulfamethoxazole were also found. This study detected six qnrA genes among Shewanella algae, ten qnrB genes among Citrobacter spp. and Escherichia coli, three oqxAB genes from Raoultella ornithinolytica and blaTEM-1 in eight E. coli strains harboring a qnrB19 gene. Our results suggest that the bacteria and antibiotic resistances/resistance genes present in bivalve mollusks depend on several factors, such as host species and respective life stage, bacterial family, farm’s location and season, and that is important to study each aquaculture farm individually to implement the most suitable measures to prevent outbreaks.
Published: 2021

111. Production of extracellular lipase from psychrotrophic bacterium Oceanisphaera sp. RSAP17 isolated from arctic soil

Author: Sukhendu Mandal, Pranab Roy, and Raihan Uddin
Subjects: food.ingredient, Tributyrin, Cellulase, Microbiology, Aeromonadaceae, chemistry.chemical_compound, Soil, food, RNA, Ribosomal, 16S, Extracellular, Agar, Amylase, Food science, Lipase, Molecular Biology, Ammonium sulfate precipitation, Phylogeny, biology, Chemistry, Temperature, General Medicine, Hydrogen-Ion Concentration, biology.organism_classification, biology.protein, Bacteria
Abstract: Cold-active extracellular lipases produced by different psychrotrophs are important for various industrial applications. We have isolated a Gram-negative, rod-shaped, aerobe, non-pigment producing psychrotrophic bacterial strain RSAP17 (MTCC 12991, MCC 4275) from the unexplored Arctic soil sample of NyAlesund, Svalbard, Norway (78° 55″ N, 11° 54″ E). The detailed morphological, biochemical, and molecular characteristics were investigated to characterize the isolate RSAP17. Analyses of the 16S rDNA sequence of strain RSAP17 (Accession no. MK391379) shows the closest match with Oceanisphaera marina YM319T (99.45%) and Oceanisphaera sediminis TW92 JCM 17329T (97.40%). The isolate is capable of producing extracellular lipase but not amylase, cellulase or urease. The optimal parameters for lipase production have been found in tributyrin based (10 mL/L) agar media supplemented with 3% (w/v) NaCl after 2-3 days of incubation at 20-22 °C temperature and pH 9 at shaking condition. We have purified the extracellular lipase from the RSAP17 grown culture supernatant through 75% ammonium sulfate precipitation followed by dialysis and DEAE cellulose column chromatography. The invitro lipolytic activity of the purified lipase enzymes has been done through zymogram analysis. The molecular weight found for the lipase is 103.8 kD. The optimal activity of the purified lipase has been found at 25 °C and pH 9. MALDI-TOF-MS study of the purified lipase showed the highest match with the sequence of prolipoprotein diacylglyceryl transferase with 44% sequence coverage. Further study on large-scale production, substrate utilization and enzymatic kinetics of this lipase could unravel its possibility in future biotechnological applications.
Published: 2021

112. Recent Findings in Escherichia coli O157:H7 Described by Researchers from Obafemi Awolowo University (Isolation and genotypic characterization of extended-spectrum beta-lactamase-producing Escherichia coli O157:H7 and Aeromonas hydrophila from...).

Abstract: For more information on this research see: Isolation and genotypic characterization of extended-spectrum beta-lactamase-producing Escherichia coli O157:H7 and Aeromonas hydrophila from selected freshwater sources in Southwest Nigeria. Keywords: Aeromonadaceae; Aeromonas; Aeromonas hydrophila; Amidohydrolases; Beta-Lactamases; Enterobacteriaceae; Enzymes and Coenzymes; Escherichia coli; Escherichia coli O157:H7; Gammaproteobacteria; Gram-Negative Bacteria; Gram-Negative Facultatively Anaerobic Rods; Health and Medicine; Lactamase; Proteobacteria; Public Health EN Aeromonadaceae Aeromonas Aeromonas hydrophila Amidohydrolases Beta-Lactamases Enterobacteriaceae Enzymes and Coenzymes Escherichia coli Escherichia coli O157:H7 Gammaproteobacteria Gram-Negative Bacteria Gram-Negative Facultatively Anaerobic Rods Health and Medicine Lactamase Proteobacteria Public Health 828 828 1 07/24/23 20230728 NES 230728 2023 JUL 28 (NewsRx) -- By a News Reporter-Staff News Editor at Genomics & Genetics Weekly -- New research on Escherichia coli O157:H7 is the subject of a new report. Aeromonadaceae, Aeromonas, Aeromonas hydrophila, Beta-Lactamases, Amidohydrolases, Enterobacteriaceae, Enzymes and Coenzymes, Escherichia coli, Escherichia coli O157:H7, Gammaproteobacteria, Gram-Negative Bacteria, Gram-Negative Facultatively Anaerobic Rods, Health and Medicine, Lactamase, Proteobacteria, Public Health. [Extracted from the article]
Published: 2023

113. Douglass Hanly Moir Pathology (DHM) Reports Findings in Aeromonas (Enteric Aeromonas Infection: a Common Enteric Bacterial Infection with a Novel Infection Pattern Detected in an Australian Population with Gastroenteritis).

Subjects: INTESTINAL infections, BACTERIAL diseases, GASTROENTERITIS, AUSTRALIANS, AEROMONAS, GRAM-negative anaerobic bacteria
Abstract: Keywords: Sydney; Australia; Australia and New Zealand; Aeromonadaceae; Aeromonas; Bacterial Infections and Mycoses; Diagnostics and Screening; Digestive System Diseases and Conditions; Gammaproteobacteria; Gastroenteritis; Gastroenterology; Gastrointestinal Diseases and Conditions; Gram-Negative Bacteria; Gram-Negative Facultatively Anaerobic Rods; Health and Medicine; Pediatrics; Proteobacteria EN Sydney Australia Australia and New Zealand Aeromonadaceae Aeromonas Bacterial Infections and Mycoses Diagnostics and Screening Digestive System Diseases and Conditions Gammaproteobacteria Gastroenteritis Gastroenterology Gastrointestinal Diseases and Conditions Gram-Negative Bacteria Gram-Negative Facultatively Anaerobic Rods Health and Medicine Pediatrics Proteobacteria 169 169 1 07/10/23 20230714 NES 230714 2023 JUL 15 (NewsRx) -- By a News Reporter-Staff News Editor at Pediatrics Week -- New research on Gram-Negative Bacteria - Aeromonas is the subject of a report. Interestingly, we discovered that species were the second most common bacterial enteric pathogens in patients with gastroenteritis, exhibiting a novel infection pattern compared to those of other enteric pathogens. [Extracted from the article]
Published: 2023

114. New Aeromonas Study Findings Recently Were Reported by Researchers at Tianjin Agricultural University [Identification and characterization of Aeromonas veronii and Vibrio cholerae from naturally diseased red swamp crayfish (Procambarus clarkia)].

Abstract: Based on phenotypic characteristics and sequence analysis of the 16S rRNA and gyrB genes, PCG1 was identified as Aeromonas veronii and PCG2 was identified as Vibrio cholerae. Keywords: Aeromonadaceae; Aeromonas; Cholera; Digestive System Diseases and Conditions; Gammaproteobacteria; Gram-Negative Bacteria; Gram-Negative Facultatively Anaerobic Rods; Proteobacteria; Vibrio cholerae; Vibrionaceae EN Aeromonadaceae Aeromonas Cholera Digestive System Diseases and Conditions Gammaproteobacteria Gram-Negative Bacteria Gram-Negative Facultatively Anaerobic Rods Proteobacteria Vibrio cholerae Vibrionaceae 733 733 1 06/19/23 20230623 NES 230623 2023 JUN 23 (NewsRx) -- By a News Reporter-Staff News Editor at Genomics & Genetics Weekly -- New study results on aeromonas have been published. [Extracted from the article]
Published: 2023

115. Qingdao Agricultural University Reports Findings in Fish Diseases and Conditions (Efficacy of bivalent vaccine against Aeromonas salmonicida and Edwardsiella tarda infections in turbot).

Subjects: AEROMONAS salmonicida, FISH diseases, EDWARDSIELLA tarda, AGRICULTURE, VACCINE effectiveness, PSETTA maxima
Abstract: Qingdao, People's Republic of China, Asia, Aeromonadaceae, Aeromonas, Aeromonas salmonicida, Animal Diseases and Conditions, Edwardsiella, Edwardsiella tarda, Enterobacteriaceae, Fish Diseases and Conditions, Gammaproteobacteria, Gram-Negative Bacteria, Gram-Negative Facultatively Anaerobic Rods, Immunology, Proteobacteria, Risk and Prevention Keywords for this news article include: Asia, Qingdao, Immunology, Aeromonadaceae, Edwardsiella tarda, Enterobacteriaceae, Gammaproteobacteria, Risk and Prevention, Aeromonas salmonicida, Gram-Negative Bacteria, People's Republic of China, Fish Diseases and Conditions, Animal Diseases and Conditions, Gram-Negative Facultatively Anaerobic Rods. Keywords: Qingdao; People's Republic of China; Asia; Aeromonadaceae; Aeromonas; Aeromonas salmonicida; Animal Diseases and Conditions; Edwardsiella; Edwardsiella tarda; Enterobacteriaceae; Fish Diseases and Conditions; Gammaproteobacteria; Gram-Negative Bacteria; Gram-Negative Facultatively Anaerobic Rods; Immunology; Proteobacteria; Risk and Prevention EN Qingdao People's Republic of China Asia Aeromonadaceae Aeromonas Aeromonas salmonicida Animal Diseases and Conditions Edwardsiella Edwardsiella tarda Enterobacteriaceae Fish Diseases and Conditions Gammaproteobacteria Gram-Negative Bacteria Gram-Negative Facultatively Anaerobic Rods Immunology Proteobacteria Risk and Prevention 31 31 1 06/19/23 20230623 NES 230623 2023 JUN 19 (NewsRx) -- By a News Reporter-Staff News Editor at Veterinary Week -- New research on Animal Diseases and Conditions - Fish Diseases and Conditions is the subject of a report. [Extracted from the article]
Published: 2023

116. Qazvin University of Medical Sciences Reports Findings in Aeromonas (An estimation of global Aeromonas infection prevalence in children with diarrhoea: a systematic review and meta-analysis).

Subjects: AEROMONAS, SCIENCE journalism, DIARRHEA, GRAM-negative anaerobic bacteria
Abstract: Iran, Asia, Aeromonadaceae, Qazvin, Aeromonas, Gammaproteobacteria, Gram-Negative Bacteria, Gram-Negative Facultatively Anaerobic Rods, Pediatrics, Proteobacteria Keywords for this news article include: Iran, Asia, Qazvin, Pediatrics, Aeromonadaceae, Gammaproteobacteria, Gram-Negative Bacteria, Gram-Negative Facultatively Anaerobic Rods. Keywords: Qazvin; Iran; Asia; Aeromonadaceae; Aeromonas; Gammaproteobacteria; Gram-Negative Bacteria; Gram-Negative Facultatively Anaerobic Rods; Pediatrics; Proteobacteria EN Qazvin Iran Asia Aeromonadaceae Aeromonas Gammaproteobacteria Gram-Negative Bacteria Gram-Negative Facultatively Anaerobic Rods Pediatrics Proteobacteria 318 318 1 06/05/23 20230609 NES 230609 2023 JUN 10 (NewsRx) -- By a News Reporter-Staff News Editor at Pediatrics Week -- New research on Gram-Negative Bacteria - Aeromonas is the subject of a report. [Extracted from the article]
Published: 2023

117. Researchers at Vellore Institute of Technology Have Reported New Data on Aeromonas hydrophila (Antibacterial Activity of Bacillus Licheniformis Isolated From Marine Sediments and Its Effect In Treating Aeromonas Hydrophila Infection In...).

Abstract: Tamil Nadu, India, Asia, Actinobacteria, Actinomyces, Actinomycetaceae, Actinomycetales, Actinomycetales Infections, Actinomycosis, Aeromonadaceae, Aeromonas, Aeromonas hydrophila, Antibacterials, Antibiotics, Antimicrobials, Drugs and Therapies, Gammaproteobacteria, Gram-Negative Bacteria, Gram-Negative Facultatively Anaerobic Rods, Gram-Positive Asporogenous Rods, Gram-Positive Bacteria, Gram-Positive Rods, Health and Medicine, Proteobacteria Keywords: Tamil Nadu; India; Asia; Actinobacteria; Actinomyces; Actinomycetaceae; Actinomycetales; Actinomycetales Infections; Actinomycosis; Aeromonadaceae; Aeromonas; Aeromonas hydrophila; Antibacterials; Antibiotics; Antimicrobials; Drugs and Therapies; Gammaproteobacteria; Gram-Negative Bacteria; Gram-Negative Facultatively Anaerobic Rods; Gram-Positive Asporogenous Rods; Gram-Positive Bacteria; Gram-Positive Rods; Health and Medicine; Proteobacteria EN Tamil Nadu India Asia Actinobacteria Actinomyces Actinomycetaceae Actinomycetales Actinomycetales Infections Actinomycosis Aeromonadaceae Aeromonas Aeromonas hydrophila Antibacterials Antibiotics Antimicrobials Drugs and Therapies Gammaproteobacteria Gram-Negative Bacteria Gram-Negative Facultatively Anaerobic Rods Gram-Positive Asporogenous Rods Gram-Positive Bacteria Gram-Positive Rods Health and Medicine Proteobacteria 1825 1825 1 05/29/23 20230602 NES 230602 2023 JUN 2 (NewsRx) -- By a News Reporter-Staff News Editor at Drug Week -- Investigators publish new report on Gram-Negative Bacteria - Aeromonas hydrophila. [Extracted from the article]
Published: 2023

118. Studies from Hainan University Yield New Information about Aeromonas (Response Regulator Kdpe Contributes To Aeromonas Dhakensis Virulence).

Abstract: According to the news reporters, the research concluded: "The Delta kdpE mutant is attenuated in virulence and shows high protective efficacy in adult zebrafish after double immunization, and is therefore a promising attenuated vaccine candidate." Keywords: Haikou; People's Republic of China; Asia; Aeromonadaceae; Aeromonas; Biological Products; Gammaproteobacteria; Gram-Negative Bacteria; Gram-Negative Facultatively Anaerobic Rods; Health and Medicine; Immunization; Proteobacteria; Vaccines EN Haikou People's Republic of China Asia Aeromonadaceae Aeromonas Biological Products Gammaproteobacteria Gram-Negative Bacteria Gram-Negative Facultatively Anaerobic Rods Health and Medicine Immunization Proteobacteria Vaccines 214 214 1 05/15/23 20230515 NES 230515 2023 MAY 17 (NewsRx) -- By a News Reporter-Staff News Editor at Vaccine Weekly -- Fresh data on Gram-Negative Bacteria - Aeromonas are presented in a new report. [Extracted from the article]
Published: 2023

119. Findings from Southwest University in the Area of Aeromonas Described [Construction and Efficacy of Aeromonas Veronii Mutant Ahcp As a Live Attenuated Vaccine for the Largemouth Bass (Micropterus Salmoides)].

Abstract: According to the news reporters, the research concluded: "These data suggest that hcp gene is an important virulence factor of A. veronii, and the live attenuated vaccine Ahcp-AV is safe and effective for prevention A. veronii infection in M. salmoides farming." Keywords: Chongqing; People's Republic of China; Asia; Aeromonadaceae; Aeromonas; Biological Products; Gammaproteobacteria; Genetics; Gram-Negative Bacteria; Gram-Negative Facultatively Anaerobic Rods; Health and Medicine; Immunization; Immunology; Proteobacteria; Risk and Prevention; Vaccines EN Chongqing People's Republic of China Asia Aeromonadaceae Aeromonas Biological Products Gammaproteobacteria Genetics Gram-Negative Bacteria Gram-Negative Facultatively Anaerobic Rods Health and Medicine Immunization Immunology Proteobacteria Risk and Prevention Vaccines 138 138 1 05/15/23 20230515 NES 230515 2023 MAY 17 (NewsRx) -- By a News Reporter-Staff News Editor at Vaccine Weekly -- Investigators discuss new findings in Gram-Negative Bacteria - Aeromonas. [Extracted from the article]
Published: 2023

120. Data on Aeromonas Detailed by Researchers at National Reference Laboratory (Bacterial Diseases of Tilapia, Their Zoonotic Potential and Risk of Antimicrobial Resistance).

Abstract: Keywords: Lelystad; Netherlands; Europe; Aeromonadaceae; Aeromonas; Bacterial Infections and Mycoses; Gammaproteobacteria; Gram-Negative Bacteria; Gram-Negative Facultatively Anaerobic Rods; Health and Medicine; Proteobacteria; Risk and Prevention; Zoonoses; Zoonosis; Zoonotic EN Lelystad Netherlands Europe Aeromonadaceae Aeromonas Bacterial Infections and Mycoses Gammaproteobacteria Gram-Negative Bacteria Gram-Negative Facultatively Anaerobic Rods Health and Medicine Proteobacteria Risk and Prevention Zoonoses Zoonosis Zoonotic 1249 1249 1 05/08/23 20230512 NES 230512 2023 MAY 12 (NewsRx) -- By a News Reporter-Staff News Editor at Health & Medicine Week -- Researchers detail new data in Gram-Negative Bacteria - Aeromonas. Then, information on emerging bacterial infections of concern for tilapia, like edwardsiellosis through Edwardsiella ictaluri and E. tarda, as well as Aeromonas schubertii is provided. [Extracted from the article]
Published: 2023

121. Data on Aeromonas Reported by Researchers at Department of Microbiology (Skin and Soft Tissue Infections due to Aeromonas spp.: An Emerging Pathogen).

Abstract: Keywords: Aeromonadaceae; Aeromonas; Drugs and Therapies; Epidemiology; Gammaproteobacteria; Gram-Negative Bacteria; Gram-Negative Facultatively Anaerobic Rods; Proteobacteria; Risk and Prevention EN Aeromonadaceae Aeromonas Drugs and Therapies Epidemiology Gammaproteobacteria Gram-Negative Bacteria Gram-Negative Facultatively Anaerobic Rods Proteobacteria Risk and Prevention 282 282 1 05/08/23 20230512 NES 230512 2023 MAY 12 (NewsRx) -- By a News Reporter-Staff News Editor at Drug Week -- New study results on aeromonas have been published. For more information on this research see: Skin and Soft Tissue Infections due to Aeromonas spp.: An Emerging Pathogen. [Extracted from the article]
Published: 2023

122. Dongshaea marina gen. nov., sp. nov., a facultatively anaerobic marine bacterium that ferments glucose with gas production

Author: Wung Yang Shieh, Tzu Yin Chen, Li-Ting Wang, Pei-Ling Wang, Chih-Lin Wei, Jwo Sheng Chen, Shih Ting Lin, Ssu Po Huang, Saulwood Lin, Yi Min Chen, and Lina Huang
Subjects: 0106 biological sciences, 0301 basic medicine, Phylogenetic tree, biology, General Medicine, 16S ribosomal RNA, biology.organism_classification, 010603 evolutionary biology, 01 natural sciences, Microbiology, 03 medical and health sciences, 030104 developmental biology, Marine bacteriophage, Botany, Gammaproteobacteria, Aeromonadaceae, Fermentation, Oceanimonas, Ecology, Evolution, Behavior and Systematics, Bacteria
Abstract: Two isolates of heterotrophic, facultatively anaerobic, marine bacteria, designated DM1 and DM2T, were recovered from a lagoon sediment sample of Dongsha Island, Taiwan. Cells were Gram-reaction-negative rods. Nearly all of the cells were non-motile and non-flagellated during the late exponential to early stationary phase of growth, while a few of the cells exhibited motility with monotrichous flagellation. The two isolates required NaCl for growth and grew optimally at about 30 °C, 2–3 % NaCl and pH 7–8. They grew aerobically and could achieve anaerobic growth by fermenting d-glucose or other carbohydrates with production of acids and the gases, including CO2 and H2. Ubiquinone Q-8 was the only respiratory quinone. Cellular fatty acids were predominated by C16 : 0, C18 : 1ω7c and C16 : 1ω7c. The major polar lipid was phosphatidylethanolamine. Strains DM1 and DM2T had DNA G+C contents of 52.0 and 51.8 mol%, respectively, as determined by HPLC analysis. Phylogenetic analyses based on 16S rRNA gene sequences clearly indicated that the two isolates formed a distinct genus-level lineage in the family Aeromonadaceae of the class Gammaproteobacteria and was an outgroup with respect to a stable supragenic clade comprising species of the genera Oceanimonas , Oceanisphaera and Zobellella . The phylogenetic data and those from chemotaxonomic, physiological and morphological characterizations support the establishment of a novel species and genus inside the family Aeromonadaceae , for which the name Dongshaea marina gen. nov., sp. nov. is proposed. The type strain is DM2T (=BCRC 81069T=JCM 32096T).
Published: 2019
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123. Organic Houttuynia cordata Thunb harbors higher abundance and diversity of antibiotic resistance genes than non-organic origin, suggesting a potential food safe risk

Author: Wenliang Xiang, Kekun Lu, Qianwen Lu, Qin Xu, and Nandi Zhang
Subjects: Organic Agriculture, Food Safety, biology, Tetracycline, Moraxellaceae, Drug Resistance, Microbial, biology.organism_classification, Enterobacteriaceae, Microbiology, Houttuynia cordata, Antibiotic resistance, hemic and lymphatic diseases, Aeromonadaceae, medicine, Houttuynia, Bacteria, Food Science, Oxalobacteraceae, medicine.drug
Abstract: The organic agricultural products has been growing rapidly in recent years. However, a potential food safe risk, resulted by introduction more antibiotic resistant genes (ARGs) accompanied with animal manure using to organic farming, has long been overlooked. In current study, the bacterial community, 22 tetracycline, 3 aminoglycoside and 4 β-lactams ARGs were respectively investigated in the organic, chemical and wild Houttuynia cordata Thunb (HCT). A total of 9 tetracycline, 3 aminoglycoside and 2 β-lactam ARG subtypes were detected, and the organic HCT harbored more ARG subtypes. The absolute and relative abundance of total ARGs in organic HCT was strikingly higher than that in chemical and wild HCT. The Enterobacteriaceae, Aeromonadaceae, Pseudomonadceae, Moraxellaceae and Oxalobacteraceae were the dominant taxa in the chemical and wild HCT, but in the organic HCT, only Enterobacteriaceae posed 83.23% - 87.40% of bacterial community. Fourteen bacterial families might be the possible hosts of ARG subtypes in the HCT. Enterobacteriaceae was a possible host of most ARG subtypes, including tetA, tetB, tetC, tetE and aadA, and it was the main bacteria affecting the behavior of ARGs in the HCT. Additionally, the tetracycline ARG subtypes had more possible hosts. These results help to better understand the ARG potential food safe risk and develop effective measures to prevent the ARG dissemination in organic agricultural product.
Published: 2019
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124. Dietary polysaccharides: fermentation potentials of a primitive gut ecosystem

Author: Lydia Zeibich, Oliver Schmidt, and Harold L. Drake
Subjects: Microorganism, Microbial metabolism, Gut flora, Polysaccharide, Microbiology, 03 medical and health sciences, Polysaccharides, RNA, Ribosomal, 16S, Aeromonadaceae, Animals, Food science, Oligochaeta, Ecosystem, Ecology, Evolution, Behavior and Systematics, 030304 developmental biology, chemistry.chemical_classification, 0303 health sciences, Bacteria, biology, 030306 microbiology, Earthworm, food and beverages, biology.organism_classification, Diet, Gastrointestinal Microbiome, Gastrointestinal Tract, chemistry, Fermentation, Lumbricus terrestris
Abstract: The alimentary canal of the earthworm is representative of primitive gut ecosystems, and gut fermenters capable of degrading ingested biomass-derived polysaccharides might contribute to the environmental impact and survival of this terrestrial invertebrate. Thus, this study evaluated the postulation that gut microbiota of the model earthworm Lumbricus terrestris ferment diverse biomass-derived polysaccharides. Structural polysaccharides (e.g. cellulose, chitin) had marginal impact on fermentation in anoxic gut content treatments. In contrast, nonstructural polysaccharides (e.g. starch, glycogen) greatly stimulated (a) the formation of diverse fermentation products (e.g. H2 , ethanol, fatty acids) and (b) the facultatively fermentative families Aeromonadaceae and Enterobacteriaceae. Despite these contrasting results with different polysaccharides, most saccharides derived from these biopolymers (e.g. glucose, N-acetylglucosamine) greatly stimulated fermentation, yielding 16S rRNA gene-based signatures of Aeromonadaceae-, Enterobacteriaceae- and Fusobacteriaceae-affiliated phylotypes. Roots and litter are dietary substrates of the earthworm, and as proof-of-principle, gut-associated fermenters responded rapidly to root- and litter-derived nutrients including saccharides. These findings suggest that (a) hydrolysis of certain ingested structural polysaccharides may be a limiting factor in the ability of gut fermenters to utilize them and (b) nonstructural polysaccharides of disrupted biomass are subject to rapid fermentation by gut microbes and yield fatty acids that can be utilized by the earthworm.
Published: 2019
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125. Discovery of α-Glucosidase Inhibitors from Marine Microorganisms: Optimization of Culture Conditions and Medium Composition

Author: Nguyen Thi Hien Trang, Kit Wayne Chew, Pau Loke Show, Nguyen Thi Trung, Do Thi Tuyen, Nguyen Thi Thao, Doris Ying Ying Tang, Nguyen Thi Thuy Linh, Nguyen Tien Cuong, Le Thanh Hoang, and Hoang Thi Yen
Subjects: 0106 biological sciences, Aquatic Organisms, Microorganism, Cell Culture Techniques, Bioengineering, Fungus, 01 natural sciences, Applied Microbiology and Biotechnology, Biochemistry, Aeromonadaceae, 03 medical and health sciences, Marine bacteriophage, 010608 biotechnology, Drug Discovery, medicine, Humans, Glycoside Hydrolase Inhibitors, Food science, Molecular Biology, IC50, 030304 developmental biology, Acarbose, 0303 health sciences, biology, Chemistry, alpha-Glucosidases, biology.organism_classification, Culture Media, Microbial population biology, Diabetes Mellitus, Type 2, Fermentation, Composition (visual arts), Biotechnology, medicine.drug
Abstract: Various studies showed that the suppression of α-glucosidase activity can impede the glucose absorption in our body, and therefore, it can be used to treat type 2 diabetes. Hence, the compounds with anti-α-glucosidase have gained considerable attention because of their potential application in diabetes treatment. In previous literature studies, these anti-α-glucosidase compounds were extracted from plants and fungus. Less studies are being conducted to identify the anti-α-glucosidase compounds in the microbial community. In this study, 23 marine bacterial strains were screened for their potential to suppress the α-glucosidase activity. The highest inhibitory activity was exhibited by isolated L06 which was identified as Oceanimonas smirnovii EBL6. The cultivation conditions, such as temperature and pH, were optimized to increase the production of α-glucosidase inhibitors by Oceanimonas smirnovii EBL6 strain. The result findings showed that the highest yield of α-glucosidase inhibitors can be obtained at the culture time of 120 h, fermentation temperature of 30 °C, and pH 4.6. Under these conditions, the inhibitory activity of α-glucosidase can reach 81%. The IC50 of n-butanol extract was 13.89 μg/ml, while standard acarbose was 31.16 μg/ml. Overall, these findings suggest that Oceanimonas smirnovii produces α-glucosidase inhibitors and could been applied in the biochemical and medicinal fields in the future.
Published: 2021

126. Aeromonas spp.: An Emerging Nosocomial Pathogen.

Author: Batra, Priyam, Mathur, Purva, and Misra, Mahesh C.
Subjects: *AEROMONADACEAE, *GRAM-negative bacteria, *AEROMONAS, *PATHOGENIC microorganisms, *BACTEREMIA
Abstract: Aeromonads are hallophillic, nonacid fast, nonspore forming, Gram-negative rods which are widely distributed in the soil, foodstuffs, and aquatic environment. Since times immemorial, they are important zoonotic pathogens of poikilotherms but are now emerging as important human pathogens. These emerging enteric pathogens flourish in the water distribution system by forming biofilms. They possess large number of virulence factors including inherent resistance to various antibiotics and ability to form biofilms using quorum sensing. These properties make them easy pathogens for human infections. Aeromonads are important enteric pathogens, but, with the growing level of immunosuppression in the population, they have been associated with various extraintestinal infections, such as skin and soft-tissue infections, traumatic wound infections, and lower respiratory tract/urinary tract infections. The average annual incidence of bacteremia in Southern Taiwan due to Aeromonas spp. was 76 cases/million inhabitants between 2008 and 2010. However, the incidence reported from Western countries is much lower. The case fatality rate among patients with Aeromonas bacteremia ranges from 27.5 to 46%. Aeromonads are universally resistant to the narrow-spectrum penicillin group of antibiotics such as penicillin, ampicillin, carbenicillin, and ticarcillin. They are however susceptible to piperacillin, azlocillin, second and third generation cephalosporins, and carbapenems. Most of the Aeromonas species are susceptible to aminoglycosides, tetracycline, chloramphenicol, trimethoprim-sulfamethoxazole, quinolones, and monobactams. This manuscript is a comprehensive systematic review of the literature available on Aeromonas spp. [ABSTRACT FROM AUTHOR]
Published: 2016
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127. Sphingomonas flavus sp. nov. isolated from road soil.

Author: Du, Juan, Singh, Hina, Won, KyungHwa, Yang, Jung-Eun, Akter, Shahina, Jin, Feng-Xie, and Yi, Tae-Hoo
Subjects: *SPHINGOMONAS, *GRAM-negative bacteria, *AEROMONADACEAE, *CHEMOTAXONOMY, *FATTY acids
Abstract: A yellow-colored, Gram-negative, strictly aerobic, non-motile, rod-shaped bacterium, designated THG-MM5, was isolated from road soil in Yongin-si, Gyeonggi-do, Republic of Korea. Based on 16S rRNA gene sequence, strain THG-MM5 was moderately related to Sphingomonas sediminicola KACC 15039 (96.1 %), Sphingomonas ginsengisoli KACC 16858 (96.1 %) and Sphingomonas jaspsi KACC 13230 (96.0 %). Chemotaxonomic data revealed that strain THG-MM5 possesses ubiquinone-10 as the only respiratory quinone, sym-homospermidine as the major polyamine and summed feature 3 (C ω7 c and/or C ω6 c), C ω7 c and C as the major fatty acids. The polar lipid profile included sphingoglycolipid. The DNA G + C content was 60.7 mol%. These data, together with phenotypic characterization, corroborated the affiliation of strain THG-MM5 to the genus Sphingomonas. Thus, the isolate represents a novel species, for which the name Sphingomonas flavus sp. nov. is proposed, with THG-MM5 as the type strain (=KACC 18277 = CCTCC AB 2014320). [ABSTRACT FROM AUTHOR]
Published: 2015
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128. A generic and systematic procedure to derive a simplified model from the anaerobic digestion model No. 1 (ADM1).

Author: Hassam, S., Ficara, E., Leva, A., and Harmand, J.
Subjects: *ANAEROBIC bacteria, *ANAEROBIC microorganisms, *AEROMONADACEAE, *ORGANIC compounds, *BIOTECHNOLOGY
Abstract: The anaerobic digestion model No.1 (ADM1) developed by the IWA Task Group for mathematical modelling of anaerobic digestion processes Batstone et al. [1] is a structural model which describes the main biochemical and physicochemical processes. For such purposes, other models have been proposed to describe anaerobic processes with a reduced set of parameters, state variables and processes. Among them, the anaerobic model No. 2 (AM2) proposed by Bernard et al. [2] which describes the degradation of soluble organic compounds, appears as a model well-suited for control and optimization applications. In this work, we aimed at obtaining a model of reduced dimensions on the basis of which to synthesize regulators or observers with guarantees of performance, stability and robustness. Specifically, our contribution is twofold. First, a modified version of the AM2 is proposed while preserving the simplicity of the new model “AM2HN”. Second, we propose a systematic and generic state association procedure in order to obtain such a simplified model from any validated ADM1. Simulations and comparisons with the predictions of the ADM1 for a case study involving the anaerobic digestion of waste sludge are presented along with satisfactory results. [ABSTRACT FROM AUTHOR]
Published: 2015
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129. Genotyping of Acinetobacter baumannii strains isolated at a Japanese hospital over five years using targeted next-generation sequencing.

Author: Alshahni, Mohamed Mahdi, Asahara, Miwa, Kawakami, Sayoko, Fujisaki, Ryuichi, Matsunaga, Naohisa, Furukawa, Taiji, Teramoto, Tamio, and Makimura, Koichi
Subjects: *ACINETOBACTER baumannii, *ACINETOBACTER, *GRAM-negative bacteria, *BACTERIA, *AEROMONADACEAE
Abstract: Acinetobacter baumannii is a Gram-negative bacterial agent involved in nosocomial infections. In this five-year retrospective study, phylogenetic relationships among carbapenem-resistant A. baumannii strains that were isolated at Teikyo University Hospital in Tokyo metropolis, Japan, were explored. A panel of 72 carbapenem-resistant A. baumannii strains that isolated from January 2006 until August 2010 was studied. Next-Generation sequencing (NGS) was employed to perform large-scale genotyping of these isolates. They were separated, according to the time of isolation, into two genetically distinct groups, one correspondent to strains of the outbreak reported to local public health department in 2010 and the other contained strains from earlier isolations, suggesting different origins of the isolates. Moreover, taxa in each group showed two main clustering patterns. Multilocus sequence typing (MLST) study on 8 isolates from the last outbreak showed that they were from one sequence type, 92, displaying less discriminatory power comparing to large-sequence typing. The clonal lineage profiles produced in this retrospective study will be used as a reference database to compare future isolations of A. baumannii . This study demonstrates the power of NGS in conducting epidemiological researches, allowing a high resolution genotyping. [ABSTRACT FROM AUTHOR]
Published: 2015
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130. Microbial community structure in a host-parasite system: the case of Prussian carp and its parasitic crustaceans

Author: Karl B. Andree, Pavel Vlasenko, O.V. Polenogova, E. N. Kashinskaya, Mikhail M. Solovyev, Evgeniy Simonov, B.A. Kiriukhin, Producció Animal, and Aqüicultura
Subjects: Carps, Corynebacterium, Zoology, микробиота кожи, medicine.disease_cause, Applied Microbiology and Biotechnology, Comamonadaceae, 03 medical and health sciences, патогенные микроорганизмы, RNA, Ribosomal, 16S, Aeromonadaceae, medicine, Animals, пресноводные рыбы, Parasites, Phylogeny, 030304 developmental biology, Prussian carp, 0303 health sciences, Lernaea, integumentary system, biology, 030306 microbiology, Host (biology), Microbiota, 16S рРНК, Pathogenic bacteria, General Medicine, biology.organism_classification, система паразит-хозяин, серебряный карась, Flavobacterium, Biotechnology
Abstract: Aims. The aim of the study was to investigate the skin microbiota of Prussian carp infested by ectoparasites from the genus Argulus and Lernaea. Methods and Results. Associated microbiota of skin of Prussian carp and ectoparasites were investigated by sequencing of the V3, V4 hypervariable regions of 16S rRNA using Illumina MiSeq sequencing platform. Conclusions. According to the Spearman rank correlation test, the increasing load of ulcerations of the skin of Prussian carp was weakly negative correlated with reduction in the abundance of the following taxa: Acrobacter, bacteria C39 (Rhodocyclaceae), Rheinheimera, Comamonadaceae, Helicobacteraceae, and Vogesella. In the present study, the microbiota of ectoparasites from genus Lernaea and Argulus were characterized for the first time. The microbiota associated with L. cyprinacea was significantly different from microbial communities of intact skin mucosa of both infested and uninfested fish and skin ulcers (ADONIS, p≤0.05). The microbiota associated with parasitic crustaceans L. cyprinacea were dominated by unclassified bacteria from Comamonadaceae, Aeromonadaceae families, and Vogesella. The dominant microbiota of A. foliaceus were represented by Flavobacterium, Corynebacterium, and unclassified Comamonadaceae. Significance and Impact of Study. Results from these studies indicate that ectoparasites have the potential to alter skin microbiota, which can play a possible role in transmission of secondary bacterial infection in fish, caused by pathogenic bacteria. info:eu-repo/semantics/acceptedVersion
Published: 2021

131. Research Conducted at Food and Drug Administration Has Updated Our Knowledge about Aeromonas hydrophila (Epidemiological Cutoff Values for Standard Broth Microdilution and Disk Diffusion Susceptibility Testing of Aeromonas Hydrophila Isolated...).

Abstract: Keywords for this news article include: Laurel, Maryland, United States, North and Central America, Aeromonadaceae, Aeromonas, Aeromonas hydrophila, Drugs and Therapies, Epidemiology, Gammaproteobacteria, Gram-Negative Bacteria, Gram-Negative Facultatively Anaerobic Rods, Proteobacteria, U.S. Food and Drug Administration, Food and Drug Administration. Keywords: Laurel; State:Maryland; United States; North and Central America; Aeromonadaceae; Aeromonas; Aeromonas hydrophila; Drugs and Therapies; Epidemiology; Gammaproteobacteria; Gram-Negative Bacteria; Gram-Negative Facultatively Anaerobic Rods; Proteobacteria; U.S. Food and Drug Administration EN Laurel State:Maryland United States North and Central America Aeromonadaceae Aeromonas Aeromonas hydrophila Drugs and Therapies Epidemiology Gammaproteobacteria Gram-Negative Bacteria Gram-Negative Facultatively Anaerobic Rods Proteobacteria U.S. Food and Drug Administration 945 945 1 04/24/23 20230428 NES 230428 2023 APR 30 (NewsRx) -- By a News Reporter-Staff News Editor at Medical Letter on the CDC & FDA -- A new study on Gram-Negative Bacteria - Aeromonas hydrophila is now available. [Extracted from the article]
Published: 2023

132. New Findings from Virginia Polytechnic Institute and State University (Virginia Tech) in the Area of Aeromonas Described (A Systematic Review of Culture-based Methods for Monitoring Antibiotic-resistant Acinetobacter, Aeromonas, and Pseudomonas...).

Abstract: For more information on this research see: A Systematic Review of Culture-based Methods for Monitoring Antibiotic-resistant Acinetobacter, Aeromonas, and Pseudomonas As Environmentally Relevant Pathogens In Wastewater and Surface Water. Keywords: Blacksburg; State:Virginia; United States; North and Central America; Acinetobacter; Aeromonadaceae; Aeromonas; Antibacterials; Antibiotics; Antimicrobials; Drugs and Therapies; Gammaproteobacteria; Gram-Negative Aerobic Rods and Cocci; Gram-Negative Bacteria; Gram-Negative Facultatively Anaerobic Rods; Health and Medicine; Moraxellaceae; Proteobacteria; Pseudomonadaceae; Pseudomonas EN Blacksburg State:Virginia United States North and Central America Acinetobacter Aeromonadaceae Aeromonas Antibacterials Antibiotics Antimicrobials Drugs and Therapies Gammaproteobacteria Gram-Negative Aerobic Rods and Cocci Gram-Negative Bacteria Gram-Negative Facultatively Anaerobic Rods Health and Medicine Moraxellaceae Proteobacteria Pseudomonadaceae Pseudomonas 1246 1246 1 04/10/23 20230414 NES 230414 2023 APR 14 (NewsRx) -- By a News Reporter-Staff News Editor at Drug Week -- Research findings on Gram-Negative Bacteria - Aeromonas are discussed in a new report. [Extracted from the article]
Published: 2023

133. Beijing Forestry University Researchers Report Research in Aeromonas (Effects of Resource Availability and Antibiotic Residues on Intestinal Antibiotic Resistance in Bellamya aeruginosa).

Abstract: Aeromonadaceae, Aeromonas, Drugs and Therapies, Gammaproteobacteria, Gram-Negative Bacteria, Gram-Negative Facultatively Anaerobic Rods, Proteobacteria Keywords: Aeromonadaceae; Aeromonas; Drugs and Therapies; Gammaproteobacteria; Gram-Negative Bacteria; Gram-Negative Facultatively Anaerobic Rods; Proteobacteria EN Aeromonadaceae Aeromonas Drugs and Therapies Gammaproteobacteria Gram-Negative Bacteria Gram-Negative Facultatively Anaerobic Rods Proteobacteria 215 215 1 04/10/23 20230414 NES 230414 2023 APR 14 (NewsRx) -- By a News Reporter-Staff News Editor at Drug Week -- Research findings on aeromonas are discussed in a new report. In particular, fragments of four opportunistic pathogens enriched in the intestine of high organic matter content sediment groups, Aeromonas hydrophila, Aeromonas caviae, Aeromonas veronii, and Aeromonas salmonicida, carried 14 ARGs. [Extracted from the article]
Published: 2023

134. Research Results from Jimei University Update Knowledge of Aeromonas salmonicida (Virulence regulation of Zn2+ uptake system znuABC on mesophilic Aeromonas salmonicida SRW-OG1).

Abstract: Keywords: Aeromonadaceae; Aeromonas; Aeromonas salmonicida; Gammaproteobacteria; Gram-Negative Bacteria; Gram-Negative Facultatively Anaerobic Rods; Proteobacteria EN Aeromonadaceae Aeromonas Aeromonas salmonicida Gammaproteobacteria Gram-Negative Bacteria Gram-Negative Facultatively Anaerobic Rods Proteobacteria 1437 1437 1 04/10/23 20230414 NES 230414 2023 APR 14 (NewsRx) -- By a News Reporter-Staff News Editor at Genomics & Genetics Weekly -- Investigators publish new report on Aeromonas salmonicida. Taken together, these indicated that the znuABC was necessary for A. salmonicida SRW-OG1 pathogenicity and environmental adaptability, and was cross regulated by iron starvation, but it was not irreplaceable for A. salmonicida SRW-OG1 Zn2+ uptake in the host." Therefore, the purpose of this study was to explore the effect of znuABC silencing on the virulence regulation of A. salmonicida SRW-OG1. [Extracted from the article]
Published: 2023

135. Investigators at Karnataka Veterinary Animal and Fisheries Sciences University Discuss Findings in Fish Diseases and Conditions [Largescale Mortality of Oreochromis Mossambicus In Lakes and Reservoirs of Karnataka Due To Coinfection of Tilapia...].

Subjects: FISH diseases, MOZAMBIQUE tilapia, FISHERY sciences, ANIMAL science, MIXED infections, GRAM-negative anaerobic bacteria, TILAPIA
Abstract: For more information on this research see: Largescale Mortality of Oreochromis Mossambicus In Lakes and Reservoirs of Karnataka Due To Coinfection of Tilapia Lake Virus (Tilv) and Multidrug-resistant Aeromonas Veronii: an Emerging Fish Disease In India. Mangalore, India, Asia, Aeromonas, Animal Diseases and Conditions, Fish Diseases and Conditions, Gammaproteobacteria, Genetics, Gram-Negative Bacteria, Gram-Negative Facultatively Anaerobic Rods, Proteobacteria, Viral RNA, Aeromonadaceae Keywords: Mangalore; India; Asia; Aeromonadaceae; Aeromonas; Animal Diseases and Conditions; Fish Diseases and Conditions; Gammaproteobacteria; Genetics; Gram-Negative Bacteria; Gram-Negative Facultatively Anaerobic Rods; Proteobacteria; Viral RNA EN Mangalore India Asia Aeromonadaceae Aeromonas Animal Diseases and Conditions Fish Diseases and Conditions Gammaproteobacteria Genetics Gram-Negative Bacteria Gram-Negative Facultatively Anaerobic Rods Proteobacteria Viral RNA 19 19 1 04/10/23 20230410 NES 230410 2023 APR 10 (NewsRx) -- By a News Reporter-Staff News Editor at Veterinary Week -- New research on Animal Diseases and Conditions - Fish Diseases and Conditions is the subject of a report. [Extracted from the article]
Published: 2023

136. Reports Outline Aeromonas hydrophila Study Results from Nanjing Agricultural University (Study of Antibacterial Properties of Cinnamaldehyde Against Aeromonas Hydrophila).

Abstract: To investigate the action mechanisms of cinnamaldehyde against A. hydrophila, we examined the antibacterial activity, bacterial membrane permeability, and ultrastructure of A. hydrophila cells treated with cinnamaldehyde." Keywords: Wuxi; People's Republic of China; Asia; Aeromonadaceae; Aeromonas; Aeromonas hydrophila; Drugs and Therapies; Gammaproteobacteria; Gram-Negative Bacteria; Gram-Negative Facultatively Anaerobic Rods; Proteobacteria EN Wuxi People's Republic of China Asia Aeromonadaceae Aeromonas Aeromonas hydrophila Drugs and Therapies Gammaproteobacteria Gram-Negative Bacteria Gram-Negative Facultatively Anaerobic Rods Proteobacteria 1559 1559 1 04/03/23 20230407 NES 230407 2023 APR 7 (NewsRx) -- By a News Reporter-Staff News Editor at Drug Week -- Data detailed on Gram-Negative Bacteria - Aeromonas hydrophila have been presented. [Extracted from the article]
Published: 2023

137. Autonomous University of the State of Hidalgo Reports Findings in Citrobacter [Bullfrogs (Lithobates catesbeianus) as a Potential Source of Foodborne Disease].

Subjects: FOODBORNE diseases, BULLFROG, CITROBACTER, STATE universities & colleges, GRAM-negative aerobic bacteria, VIBRIO anguillarum
Abstract: Our news editors obtained a quote from the research from the Autonomous University of the State of Hidalgo, "The present study aimed to identify bacteria that cause foodborne diseases or are associated with human diseases. Keywords: Hidalgo; Mexico; North and Central America; Acinetobacter; Aeromonadaceae; Aeromonas; Citrobacter; Enterobacteriaceae; Food Poisoning; Food Safety; Foodborne Diseases and Conditions; Gammaproteobacteria; Gram-Negative Aerobic Rods and Cocci; Gram-Negative Bacteria; Gram-Negative Facultatively Anaerobic Rods; Moraxellaceae; Proteobacteria EN Hidalgo Mexico North and Central America Acinetobacter Aeromonadaceae Aeromonas Citrobacter Enterobacteriaceae Food Poisoning Food Safety Foodborne Diseases and Conditions Gammaproteobacteria Gram-Negative Aerobic Rods and Cocci Gram-Negative Bacteria Gram-Negative Facultatively Anaerobic Rods Moraxellaceae Proteobacteria 8 8 1 04/03/23 20230406 NES 230406 2023 APR 6 (NewsRx) -- By a News Reporter-Staff News Editor at Food Weekly News -- New research on Gram-Negative Bacteria - Citrobacter is the subject of a report. [Extracted from the article]
Published: 2023

138. Neijiang Normal University Researchers Describe Research in Aeromonas hydrophila (Immunogenicity and protective efficacy of OmpA subunit vaccine against Aeromonas hydrophila infection in Megalobrama amblycephala: An effective alternative to the...).

Abstract: Aeromonadaceae, Aeromonas, Aeromonas hydrophila, Gammaproteobacteria, Gram-Negative Bacteria, Gram-Negative Facultatively Anaerobic Rods, Immunology, Proteobacteria, Risk and Prevention In conclusion, these results indicated that both the inactivated vaccine and OmpA subunit vaccine could protect juvenile M. amblycephala against A. hydrophila infection, of which OmpA subunit vaccine provided more effective immune protection and can be used as an ideal candidate for the A. hydrophila vaccine." Keywords: Aeromonadaceae; Aeromonas; Aeromonas hydrophila; Gammaproteobacteria; Gram-Negative Bacteria; Gram-Negative Facultatively Anaerobic Rods; Immunology; Proteobacteria; Risk and Prevention EN Aeromonadaceae Aeromonas Aeromonas hydrophila Gammaproteobacteria Gram-Negative Bacteria Gram-Negative Facultatively Anaerobic Rods Immunology Proteobacteria Risk and Prevention 3002 3002 1 03/27/23 20230331 NES 230331 2023 MAR 31 (NewsRx) -- By a News Reporter-Staff News Editor at Health & Medicine Week -- Research findings on Aeromonas hydrophila are discussed in a new report. [Extracted from the article]
Published: 2023

139. Researchers at Medical School of Chinese People's Liberation Army (PLA) Target Aeromonas (Epidemiological characteristics, virulence potential, antimicrobial resistance profiles, and phylogenetic analysis of Aeromonas caviae isolated from...).

Abstract: It may contribute to the management of extra-intestinal infections as well as the prevention and control of drug resistance." Keywords: Aeromonadaceae; Aeromonas; Antimicrobial Resistance; Antimicrobials; Drug Resistance; Drugs and Therapies; Enzymes and Coenzymes; Epidemiology; Gammaproteobacteria; Genetics; Gram-Negative Bacteria; Gram-Negative Facultatively Anaerobic Rods; Health and Medicine; Lactamase; Nosocomial Diseases and Conditions; Nosocomial Infections; Proteobacteria; Risk and Prevention EN Aeromonadaceae Aeromonas Antimicrobial Resistance Antimicrobials Drug Resistance Drugs and Therapies Enzymes and Coenzymes Epidemiology Gammaproteobacteria Genetics Gram-Negative Bacteria Gram-Negative Facultatively Anaerobic Rods Health and Medicine Lactamase Nosocomial Diseases and Conditions Nosocomial Infections Proteobacteria Risk and Prevention 2182 2182 1 03/23/23 20230317 NES 230317 2023 MAR 17 (NewsRx) -- By a News Reporter-Staff News Editor at Drug Week -- Research findings on aeromonas are discussed in a new report. [Extracted from the article]
Published: 2023

140. Reports Summarize Aeromonas hydrophila Study Results from Chinese Academy of Fishery Sciences (Unraveling the Antibacterial Mechanism of Lactiplantibacillus Plantarum My2 Cell-free Supernatants Against Aeromonas Hydrophila St3 and Potential ...).

Subjects: AEROMONAS hydrophila, FISHERY sciences, GRAM-negative bacteria, GRAM-negative anaerobic bacteria, KREBS cycle
Abstract: Keywords: Guangzhou; People's Republic of China; Asia; Aeromonadaceae; Aeromonas; Aeromonas hydrophila; Food Safety; Gammaproteobacteria; Gram-Negative Bacteria; Gram-Negative Facultatively Anaerobic Rods; Proteobacteria EN Guangzhou People's Republic of China Asia Aeromonadaceae Aeromonas Aeromonas hydrophila Food Safety Gammaproteobacteria Gram-Negative Bacteria Gram-Negative Facultatively Anaerobic Rods Proteobacteria 2023 MAR 9 (NewsRx) -- By a News Reporter-Staff News Editor at Food Weekly News -- Investigators publish new report on Gram-Negative Bacteria - Aeromonas hydrophila. According to news reporting out of Guangzhou, People's Republic of China, by NewsRx editors, research stated, "Aeromonas hydrophila is a pathogenic and spoilage bacterium, frequently found in aquatic products, demon-strated to cause foodborne illness and food spoilage. [Extracted from the article]
Published: 2023

141. Researchers' Work from Islamic Azad University Focuses on Aeromonas hydrophila (The Prevalence of Bla Vim, Bla Kpc, Bla Ndm, Bla Imp, Bla Shv, Bla Tem, Bla Ctx-m, and Class I and Ii Integrons Genes In Aeromonas Hydrophila Isolated From Clinical...).

Abstract: Keywords for this news article include: Qom, Iran, Aeromonadaceae, Aeromonas, Aeromonas hydrophila, Drugs and Therapies, Enzymes and Coenzymes, Gammaproteobacteria, Genetics, Gram-Negative Bacteria, Gram-Negative Facultatively Anaerobic Rods, Lactamase, Proteobacteria, Islamic Azad University. Qom, Iran, Aeromonadaceae, Aeromonas, Aeromonas hydrophila, Drugs and Therapies, Enzymes and Coenzymes, Genetics, Gram-Negative Bacteria, Gram-Negative Facultatively Anaerobic Rods, Lactamase, Proteobacteria, Gammaproteobacteria Keywords: Qom; Iran; Aeromonadaceae; Aeromonas; Aeromonas hydrophila; Drugs and Therapies; Enzymes and Coenzymes; Gammaproteobacteria; Genetics; Gram-Negative Bacteria; Gram-Negative Facultatively Anaerobic Rods; Lactamase; Proteobacteria EN Qom Iran Aeromonadaceae Aeromonas Aeromonas hydrophila Drugs and Therapies Enzymes and Coenzymes Gammaproteobacteria Genetics Gram-Negative Bacteria Gram-Negative Facultatively Anaerobic Rods Lactamase Proteobacteria 1485 1485 1 03/24/23 20230224 NES 230224 2023 FEB 24 (NewsRx) -- By a News Reporter-Staff News Editor at Drug Week -- Investigators publish new report on Gram-Negative Bacteria - Aeromonas hydrophila. [Extracted from the article]
Published: 2023

142. Henan University of Science and Technology Researchers Focus on Aeromonas (Genetic characterization and virulence determinants of multidrug-resistant NDM-1-producing Aeromonas caviae).

Abstract: Keywords: Aeromonadaceae; Aeromonas; Drugs and Therapies; Gammaproteobacteria; Genetics; Gram-Negative Bacteria; Gram-Negative Facultatively Anaerobic Rods; Proteobacteria EN Aeromonadaceae Aeromonas Drugs and Therapies Gammaproteobacteria Genetics Gram-Negative Bacteria Gram-Negative Facultatively Anaerobic Rods Proteobacteria 2023 FEB 3 (NewsRx) -- By a News Reporter-Staff News Editor at Drug Week -- Researchers detail new data in aeromonas. Aeromonadaceae, Aeromonas, Drugs and Therapies, Gammaproteobacteria, Genetics, Gram-Negative Bacteria, Gram-Negative Facultatively Anaerobic Rods, Proteobacteria. [Extracted from the article]
Published: 2023

143. College of Food Science and Technology Researchers Illuminate Research in Aeromonas (Evaluation of Antibacterial and Antibiofilm Properties of Kojic Acid against Aeromonas sobria and Staphylococcus saprophyticus).

Abstract: Keywords: Aeromonadaceae; Aeromonas; Bacillales; Drugs and Therapies; Gammaproteobacteria; Gram-Negative Bacteria; Gram-Negative Facultatively Anaerobic Rods; Gram-Positive Bacteria; Gram-Positive Cocci; Gram-Positive Endospore-Forming Rods; Proteobacteria; Staphylococcaceae; Staphylococcus EN Aeromonadaceae Aeromonas Bacillales Drugs and Therapies Gammaproteobacteria Gram-Negative Bacteria Gram-Negative Facultatively Anaerobic Rods Gram-Positive Bacteria Gram-Positive Cocci Gram-Positive Endospore-Forming Rods Proteobacteria Staphylococcaceae Staphylococcus 2023 FEB 3 (NewsRx) -- By a News Reporter-Staff News Editor at Drug Week -- Research findings on aeromonas are discussed in a new report. Aeromonas, Bacillales, Drugs and Therapies, Gammaproteobacteria, Gram-Negative Bacteria, Gram-Negative Facultatively Anaerobic Rods, Gram-Positive Bacteria, Gram-Positive Cocci, Gram-Positive Endospore-Forming Rods, Proteobacteria, Staphylococcaceae, Staphylococcus, Aeromonadaceae. [Extracted from the article]
Published: 2023

144. Complete genome sequence of Tolumonas auensis type strain (TA 4T)

Author: Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute]
Published: 2011
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145. Treatment of wastewater effluents from Bogotá – Colombia by the photo-electro-Fenton process: Elimination of bacteria and pharmaceutical

Author: Diana Martínez-Pachón, Rodrigo A. Echeverry-Gallego, Félix Hernández, Ricardo A. Torres-Palma, Efraím A. Serna-Galvis, José M. Villarreal, Ana María Botero-Coy, and Alejandro Moncayo-Lasso
Subjects: Environmental Engineering, 010504 meteorology & atmospheric sciences, Iron, Colombia, Wastewater, 010501 environmental sciences, 01 natural sciences, Tandem Mass Spectrometry, medicine, Aeromonadaceae, Humans, Environmental Chemistry, Waste Management and Disposal, Effluent, Norfloxacin, 0105 earth and related environmental sciences, Bacteria, biology, Chemistry, Sulfamethoxazole, DNA degradation, advanced oxidation processes, pollutants degradation, real wastewater treatment, Hydrogen Peroxide, biology.organism_classification, Pollution, Trimethoprim, Lincomycin, bacteria inactivation, Ciprofloxacin, Pharmaceutical Preparations, Environmental chemistry, bacteria and pharmaceuticals occurrence, Oxidation-Reduction, Water Pollutants, Chemical, Chromatography, Liquid, medicine.drug
Abstract: In this work, the occurrences of bacteria families and relevant pharmaceuticals in municipal wastewater effluents from Bogota (Colombia), and their treatment by the photo-electro-Fenton process were studied. Twenty-five representative pharmaceuticals (azithromycin, carbamazepine, ciprofloxacin, clarithromycin, diclofenac, enalapril, gabapentin, iopromide, metoprolol, sulfamethoxazole, trimethoprim, valsartan, clindamycin, erythromycin, levamisole, lincomycin, norfloxacin, oxolinic acid, phenazone, primidone, salbutamol, sulfadiazine, tetracycline, tramadol, and venlafaxine) were quantified in the effluent by LC-MS/MS analysis. Four of these target compounds (azithromycin, diclofenac, trimethoprim, norfloxacin) were found at concentrations that represent an environmental risk. In addition, several bacteria families related to water and foodborne diseases were identified in such effluents (e.g., Pseudomonadaceae, Campylobacteraceae, Aeromonadaceae, Enterobacteriaceae, and Bacteroidaceae), via shotgun-metagenomic technique. Then, a bench-scale photo-electro-Fenton (PEF) system equipped with a DSA anode (Ti/IrO2-SnO2) and a GDE cathode was applied to treat such effluents. After 60 min, this treatment led to a decrease in the ratio of the bacterial content in the original samples, ~150 thousand times, and a pondered removal of 66.12% for the pharmaceuticals. The study of the process pathways indicated that the bacteria and pharmaceuticals elimination mainly occurred through attacks of hydroxyl and chlorine radicals. Interestingly, in the case of pharmaceuticals, their environmental risk quotients were diminished after the PEF application. Furthermore, the prolonged action of this electrochemical process induced ~15% of mineralization and a significant reduction of the total DNA (removal >85%). Hence, the photo-electro-Fenton process showed to be a promising alternative to deal with municipal effluents for limiting the waterborne diseases, pollution by pharmaceuticals, and mobility/availability of genetic material coming from microorganisms.
Published: 2021

146. CHROMagar mSuperCARBA performance in carbapenem-resistant Enterobacteriaceae isolates characterized at molecular level and routine surveillance rectal swab specimens.

Author: García-Fernández, Sergio, Hernández-García, Marta, Valverde, Aránzazu, Ruiz-Garbajosa, Patricia, Morosini, María Isabel, and Cantón, Rafael
Subjects: *GRAM-negative bacteria, *AEROMONADACEAE, *ENTEROBACTERIACEAE, *CARBAPENEMS, *CITROBACTER
Abstract: Performance of the CHROMagar mSuperCARBA media was assessed in both well-characterized carbapenem-resistant Enterobacteriaceae ( n = 52) and routine surveillance rectal swab specimens ( n = 211). Limit of detection ranged between 10 1 and 10 2 CFU/mL except for OXA-48 producers with low-carbapenem MICs (10 6 CFU/mL). High sensitivity (100%) and specificity (100%) were obtained with rectal swabs. [ABSTRACT FROM AUTHOR]
Published: 2017
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147. Expression and characterization of catechol 1,2-dioxygenase from Oceanimonas marisflavi 102-Na3

Author: Jing Li, Meiwen Cao, Jianguo Liu, and Ziyi Li
Subjects: Genetic Vectors, Catechols, Gene Expression, Pyrogallol, medicine.disease_cause, Substrate Specificity, Aeromonadaceae, chemistry.chemical_compound, Bacterial Proteins, Dioxygenase, medicine, Escherichia coli, Catechol 1,2-dioxygenase, Oceanimonas, Amino Acid Sequence, Cloning, Molecular, Phylogeny, chemistry.chemical_classification, Catechol, Molecular mass, biology, Sequence Homology, Amino Acid, Chemistry, Hydrogen-Ion Concentration, biology.organism_classification, Catechol 1,2-Dioxygenase, Recombinant Proteins, Molecular Weight, Kinetics, Enzyme, Biochemistry, bacteria, Protein Multimerization, Sequence Alignment, Biotechnology
Abstract: The gene of catechol 1, 2-dioxygenase was identified and cloned from the genome of Oceanimonas marisflavi 102-Na3. The protein was expressed in Escherichia coli BL21 (DE3) and purified to homogeneity of a dimer with molecular mass of 69.2 kDa. The enzyme was highly stable in pH 6.0–9.5 and below 45 °C and exhibited the maximum activity at pH 8.0 and 30 °C. Being the first characterized intradiol dioxygenase from marine bacteria Oceanimonas sp., the enzyme showed catalytic activity for catechol, 3-methylcatechol, 4-methylcatechol, 3-chlorocatechol, 4-chlorocatechol and pyrogallol. For catechol, Km and Vmax were 11.2 μM and 13.4 U/mg of protein, respectively. The enzyme also showed resistance to most of the metal ions, surfactants and organic solvents, being a promising biocatalyst for biodegradation of aromatic compounds in complex environments.
Published: 2020

148. Properties of Hemolysin and Protease Produced by Aeromonas trota.

Author: Takahashi, Eizo, Ozaki, Haruka, Fujii, Yoshio, Kobayashi, Hidetomo, Yamanaka, Hiroyasu, Arimoto, Sakae, Negishi, Tomoe, and Okamoto, Keinosuke
Subjects: *HEMOLYSIS & hemolysins, *PROTEOLYTIC enzymes, *AEROMONAS, *EXOTOXIN, *AEROMONADACEAE, *IMMUNOBLOTTING
Abstract: We examined the properties of exotoxins produced by Aeromonas trota (A. enteropelogenes), one of the diarrheagenic species of Aeromonadaceae. Nine of 19 A. trota isolates that grew on solid media containing erythrocytes showed hemolytic activity. However, the hemolytic activities of the culture supernatants of these hemolytic strains of A. trota were markedly lower than those of A. sobria when cultured in liquid medium, and the amount of hemolysin detected by immunoblotting using antiserum against the hemolysin produced by A. sobria was also low. A mouse intestine loop assay using living bacterial cells showed that A. trota 701 caused the significant accumulation of fluid, and antiserum against the hemolysin produced suppressed the enterotoxic action of A. trota 701. These results indicated that A. trota 701 was diarrheagenic and the hemolysin produced was the causative agent of the enterotoxic activity of A. trota. The hemolysin in A. sobria was previously shown to be secreted in a preform (inactive form) and be activated when the carboxy-terminal domain was cleaved off by proteases in the culture supernatant. Since mature hemolysin was detected in the culture supernatants of A. trota, we analyzed the extracellular protease produced by A. trota. Fifteen of 19 A. trota isolates that grew on solid media containing skim milk showed proteolytic activity. We subsequently found that most A. trota isolates possessed the serine protease gene, but not the metalloprotease gene. Therefore, we determined the nucleotide sequence of the serine protease gene and its chaperone A. trota gene. The results obtained revealed that the deduced amino acid sequences of serine protease and the chaperone were homologous to those of A. sobria with identities of 83.0% and 75.8%, respectively. [ABSTRACT FROM AUTHOR]
Published: 2014
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149. Properties of Hemolysin and Protease Produced by Aeromonas trota.

Author: Takahashi, Eizo, Ozaki, Haruka, Fujii, Yoshio, Kobayashi, Hidetomo, Yamanaka, Hiroyasu, Arimoto, Sakae, Negishi, Tomoe, and Okamoto, Keinosuke
Subjects: HEMOLYSIS & hemolysins, PROTEOLYTIC enzymes, AEROMONAS, EXOTOXIN, AEROMONADACEAE, IMMUNOBLOTTING
Abstract: We examined the properties of exotoxins produced by Aeromonas trota (A. enteropelogenes), one of the diarrheagenic species of Aeromonadaceae. Nine of 19 A. trota isolates that grew on solid media containing erythrocytes showed hemolytic activity. However, the hemolytic activities of the culture supernatants of these hemolytic strains of A. trota were markedly lower than those of A. sobria when cultured in liquid medium, and the amount of hemolysin detected by immunoblotting using antiserum against the hemolysin produced by A. sobria was also low. A mouse intestine loop assay using living bacterial cells showed that A. trota 701 caused the significant accumulation of fluid, and antiserum against the hemolysin produced suppressed the enterotoxic action of A. trota 701. These results indicated that A. trota 701 was diarrheagenic and the hemolysin produced was the causative agent of the enterotoxic activity of A. trota. The hemolysin in A. sobria was previously shown to be secreted in a preform (inactive form) and be activated when the carboxy-terminal domain was cleaved off by proteases in the culture supernatant. Since mature hemolysin was detected in the culture supernatants of A. trota, we analyzed the extracellular protease produced by A. trota. Fifteen of 19 A. trota isolates that grew on solid media containing skim milk showed proteolytic activity. We subsequently found that most A. trota isolates possessed the serine protease gene, but not the metalloprotease gene. Therefore, we determined the nucleotide sequence of the serine protease gene and its chaperone A. trota gene. The results obtained revealed that the deduced amino acid sequences of serine protease and the chaperone were homologous to those of A. sobria with identities of 83.0% and 75.8%, respectively. [ABSTRACT FROM AUTHOR]
Published: 2014
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150. Lignin induced iron reduction by novel sp., Tolumonas lignolytic BRL6-1

Author: Carrie D. Nicora, Young-Mo Kim, Rosalie K. Chu, Andrew F. Billings, Lani DeDiego, Kristen M. DeAngelis, Joshua N. Adkins, Blake A. Simmons, Roberto Orellana, Gina Chaput, and Cullen, Daniel
Subjects: Metabolic Processes, Protein Folding, Lignin, Biochemistry, chemistry.chemical_compound, Electrochemistry, Macromolecular Structure Analysis, Metabolites, Biomass, Protein Metabolism, Gel Electrophoresis, chemistry.chemical_classification, Staining, 0303 health sciences, Multidisciplinary, biology, Chemical Reactions, food and beverages, Chemistry, Physical Sciences, Medicine, Anaerobic bacteria, Sulfatases, Radical SAM, Oxidation-Reduction, Glycolysis, Peroxidase, Research Article, Protein Structure, Silver Staining, food.ingredient, General Science & Technology, Science, Radical, Iron, macromolecular substances, Anaerobic Bacteria, Electrophoretic Staining, Research and Analysis Methods, complex mixtures, Aeromonadaceae, 03 medical and health sciences, Electrophoretic Techniques, food, Bacterial Proteins, Cellulose, Molecular Biology, 030304 developmental biology, Bacteria, 030306 microbiology, fungi, technology, industry, and agriculture, Chemical Compounds, Organisms, Biology and Life Sciences, Proteins, Enzyme, Metabolism, chemistry, Specimen Preparation and Treatment, Tolumonas, biology.protein, Oxidation-Reduction Reactions
Abstract: Lignin is the second most abundant carbon polymer on earth and despite having more fuel value than cellulose, it currently is considered a waste byproduct in many industrial lignocellulose applications. Valorization of lignin relies on effective and green methods of de-lignification, with a growing interest in the use of microbes. Here we investigate the physiology and molecular response of the novel facultative anaerobic bacterium, Tolumonas lignolytica BRL6-1, to lignin under anoxic conditions. Physiological and biochemical changes were compared between cells grown anaerobically in either lignin-amended or unamended conditions. In the presence of lignin, BRL6-1 accumulates higher biomass and has a shorter lag phase compared to unamended conditions, and 14% of the proteins determined to be significantly higher in abundance by log2 fold-change of 2 or greater were related to Fe(II) transport in late logarithmic phase. Ferrozine assays of the supernatant confirmed that Fe(III) was bound to lignin and reduced to Fe(II) only in the presence of BRL6-1, suggesting redox activity by the cells. LC-MS/MS analysis of the secretome showed an extra band at 20 kDa in lignin-amended conditions. Protein sequencing of this band identified a protein of unknown function with homology to enzymes in the radical SAM superfamily. Expression of this protein in lignin-amended conditions suggests its role in radical formation. From our findings, we suggest that BRL6-1 is using a protein in the radical SAM superfamily to interact with the Fe(III) bound to lignin and reducing it to Fe(II) for cellular use, increasing BRL6-1 yield under lignin-amended conditions. This interaction potentially generates organic free radicals and causes a radical cascade which could modify and depolymerize lignin. Further research should clarify the extent to which this mechanism is similar to previously described aerobic chelator-mediated Fenton chemistry or radical producing lignolytic enzymes, such as lignin peroxidases, but under anoxic conditions.
Published: 2020

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