101. Identification of primary and secondary metabolites and transcriptome profile of soybean tissues during different stages of hypoxia
- Author
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Christian Richter, Silas Aurelian Döpp, Larissa Alexandra Cardoso Moraes, Juliana Marcolino-Gomes, Harald Schwalbe, Liliane Marcia Mert Henning, Alexandre Lima Nepomuceno, Luiz Alberto Colnago, Isabel Coutinho, LILIANE MARCIA MERTZ HENNING, CNPSO, ALEXANDRE LIMA NEPOMUCENO, CNPSO, LARISSA ALEXANDRA CARDOSO MORAES, CNPSO, and LUIZ ALBERTO COLNAGO, CNPDIA.
- Subjects
0106 biological sciences ,0301 basic medicine ,Soja ,Primary metabolites ,Computational biology ,lcsh:Computer applications to medicine. Medical informatics ,Mass spectrometry ,01 natural sciences ,Metabolitos primários ,Nuclear magnetic resonance ,Transcriptome ,03 medical and health sciences ,Metabolomics ,lcsh:Science (General) ,Gene ,Multidisciplinary ,Chemistry ,Secondary metabolites ,Ressonância magnética nuclear ,Primary metabolite ,Pulse sequence ,Economics, Econometrics and Finance ,030104 developmental biology ,Metabólitos secundários ,ddc:540 ,Proton NMR ,lcsh:R858-859.7 ,Soybean ,Two-dimensional nuclear magnetic resonance spectroscopy ,lcsh:Q1-390 ,010606 plant biology & botany - Abstract
NMR and chromatography methods combined with mass spectrometry are the most important analytical techniques employed for plant metabolomics screening. Metabolomic analysis integrated to transcriptome screening add an important extra dimension to the information flow from DNA to RNA to protein. The most useful NMR experiment in metabolomics analysis is the proton spectra due the high receptivity of 1H and important structural information, through proton–proton scalar coupling. Routinely, databases have been used in identification of primary metabolites, however, there is currently no comparable data for identification of secondary metabolites, mainly, due to signal overlap in normal 1H NMR spectra and natural variation of plant. Related to spectra overlap, alternatively, better resolution can be find using 1H pure shift and 2D NMR pulse sequence in complex samples due to spreading the resonances in a second dimension. Thus, in data brief we provide a catalogue of metabolites and expression levels of genes identified in soy leaves and roots under flooding stress. Keywords: Soybean, Nuclear magnetic resonance, Primary metabolites, Secondary metabolites
- Published
- 2018