3,553 results on '"Baker, Timothy"'
Search Results
102. Current Challenges in the Management of Sepsis in ICUs in Resource-Poor Settings and Suggestions for the Future
- Author
-
Schultz, Marcus J., Dünser, Martin W., Dondorp, Arjen M., Adhikari, Neill K. J., Iyer, Shivakumar, Kwizera, Arthur, Lubell, Yoel, Papali, Alfred, Pisani, Luigi, Riviello, Elisabeth D., Angus, Derek C., Azevedo, Luciano C., Baker, Timothy, Diaz, Janet V., Festic, Emir, Haniffa, Rashan, Jawa, Randeep, Jacob, Shevin T., Kissoon, Niranjan, Lodha, Rakesh, Martin-Loeches, Ignacio, Lundeg, Ganbold, Misango, David, Mer, Mervyn, Mohanty, Sanjib, Murthy, Srinivas, Musa, Ndidiamaka, Nakibuuka, Jane, Neto, Ary Serpa, Mai, NT Hoang, Thien, Binh Nguyen, Pattnaik, Rajyabardhan, Phua, Jason, Preller, Jacobus, Povoa, Pedro, Ranjit, Suchitra, Talmor, Daniel, Thevanayagam, Jonarthan, Thwaites, C. Louise, Dondorp, Arjen M., editor, Dünser, Martin W., editor, and Schultz, Marcus J., editor
- Published
- 2019
- Full Text
- View/download PDF
103. Author Correction: Mapping peat thickness and carbon stocks of the central Congo Basin using field data
- Author
-
Crezee, Bart, Dargie, Greta C., Ewango, Corneille E. N., Mitchard, Edward T. A., Emba B., Ovide, Kanyama T., Joseph, Bola, Pierre, Ndjango, Jean-Bosco N., Girkin, Nicholas T., Bocko, Yannick E., Ifo, Suspense A., Hubau, Wannes, Seidensticker, Dirk, Batumike, Rodrigue, Imani, Gérard, Cuní-Sanchez, Aida, Kiahtipes, Christopher A., Lebamba, Judicaël, Wotzka, Hans-Peter, Bean, Hollie, Baker, Timothy R., Baird, Andy J., Boom, Arnoud, Morris, Paul J., Page, Susan E., Lawson, Ian T., and Lewis, Simon L.
- Published
- 2022
- Full Text
- View/download PDF
104. Patterns and drivers of development in a west Amazonian peatland during the late Holocene
- Author
-
Kelly, Thomas J., Lawson, Ian T., Roucoux, Katherine H., Baker, Timothy R., and Honorio Coronado, Euridice N.
- Published
- 2020
- Full Text
- View/download PDF
105. Psychiatric comorbidities in a comparative effectiveness smoking cessation trial: Relations with cessation success, treatment response, and relapse risk factors
- Author
-
Johnson, Adrienne L., Kaye, Jesse, Baker, Timothy B., Fiore, Michael C., Cook, Jessica W., and Piper, Megan E.
- Published
- 2020
- Full Text
- View/download PDF
106. Structural Rearrangements in R432A Variant of AAV2 Affect Genome Packaging
- Author
-
Drouin, Lauren M, Lins, Bridget, Janssen, Mandy, Bennett, Antonette, Chipman, Paul R, McKenna, Robert, Chen, Weijun, Muzyczka, Nicholas, Cardone, Giovanni, Baker, Timothy S, and Agbandje-McKenna, Mavis
- Subjects
Microscopy ,Condensed Matter Physics ,Biochemistry and Cell Biology ,Materials Engineering - Published
- 2016
107. The C-Terminal Arm of the Human Papillomavirus Major Capsid Protein Is Immunogenic and Involved in Virus-Host Interaction
- Author
-
Li, Zhihai, Yan, Xiaodong, Yu, Hai, Wang, Daning, Song, Shuo, Li, Yunbing, He, Maozhou, Hong, Qiyang, Zheng, Qingbing, Zhao, Qinjian, Gu, Ying, Zhang, Jun, Janssen, Mandy EW, Cardone, Giovanni, Olson, Norman H, Baker, Timothy S, Li, Shaowei, and Xia, Ningshao
- Subjects
Cancer ,Immunization ,Cervical Cancer ,Infectious Diseases ,Vaccine Related ,Sexually Transmitted Infections ,Aetiology ,2.2 Factors relating to the physical environment ,2.1 Biological and endogenous factors ,Infection ,Capsid Proteins ,Cell Line ,Tumor ,Cryoelectron Microscopy ,Crystallography ,X-Ray ,Female ,Host-Pathogen Interactions ,Humans ,Models ,Molecular ,Molecular Dynamics Simulation ,Oncogene Proteins ,Viral ,Papillomaviridae ,Protein Conformation ,Uterine Cervical Neoplasms ,Biophysics - Abstract
Cervical cancer is the second most prevalent malignant tumor among women worldwide. High-risk human papillomaviruses (HPVs) are believed to be the major causative pathogens of mucosal epithelial cancers including cervical cancer. The HPV capsid is made up of 360 copies of major (L1) and 72 copies of minor (L2) capsid proteins. To date, limited high-resolution structural information about the HPV capsid has hindered attempts to understand details concerning the mechanisms by which HPV assembles and infects cells. In this study, we have constructed a pseudo-atomic model of the HPV59 L1-only capsid and demonstrate that the C-terminal arm of L1 participates in virus-host interactions. Moreover, when conjugated to a scaffold protein, keyhole limpet hemocyanin (KLH), this arm is immunogenic in vivo. These results provide new insights that will help elucidate HPV biology, and hence pave a way for the design of next-generation HPV vaccines.
- Published
- 2016
108. Self-assembly of coherently dynamic, auxetic, two-dimensional protein crystals.
- Author
-
Suzuki, Yuta, Cardone, Giovanni, Restrepo, David, Zavattieri, Pablo D, Baker, Timothy S, and Tezcan, F Akif
- Subjects
Escherichia coli ,Disulfides ,Metals ,Aldehyde-Lyases ,Microscopy ,Electron ,Transmission ,Crystallization ,Protein Conformation ,Pliability ,Stress ,Mechanical ,Rotation ,Thermodynamics ,Models ,Molecular ,Mutant Proteins ,Microscopy ,Electron ,Transmission ,Stress ,Mechanical ,Models ,Molecular ,General Science & Technology - Abstract
Two-dimensional (2D) crystalline materials possess unique structural, mechanical and electronic properties that make them highly attractive in many applications. Although there have been advances in preparing 2D materials that consist of one or a few atomic or molecular layers, bottom-up assembly of 2D crystalline materials remains a challenge and an active area of development. More challenging is the design of dynamic 2D lattices that can undergo large-scale motions without loss of crystallinity. Dynamic behaviour in porous three-dimensional (3D) crystalline solids has been exploited for stimuli-responsive functions and adaptive behaviour. As in such 3D materials, integrating flexibility and adaptiveness into crystalline 2D lattices would greatly broaden the functional scope of 2D materials. Here we report the self-assembly of unsupported, 2D protein lattices with precise spatial arrangements and patterns using a readily accessible design strategy. Three single- or double-point mutants of the C4-symmetric protein RhuA were designed to assemble via different modes of intermolecular interactions (single-disulfide, double-disulfide and metal-coordination) into crystalline 2D arrays. Owing to the flexibility of the single-disulfide interactions, the lattices of one of the variants ((C98)RhuA) are essentially defect-free and undergo substantial, but fully correlated, changes in molecular arrangement, yielding coherently dynamic 2D molecular lattices. (C98)RhuA lattices display a Poisson's ratio of -1-the lowest thermodynamically possible value for an isotropic material-making them auxetic.
- Published
- 2016
109. Fully automated pipeline for measurement of the thoracic aorta using joint segmentation and localization neural network
- Author
-
Katakol, Sudeep, primary, Baker, Timothy J., additional, Bian, Zhangxing, additional, Lu, Yanglong, additional, Spahlinger, Greg, additional, Hatt, Charles R., additional, and Burris, Nicholas S., additional
- Published
- 2023
- Full Text
- View/download PDF
110. The global biogeography of tree leaf form and habit
- Author
-
Ma, Haozhi, primary, Crowther, Thomas W., additional, Mo, Lidong, additional, Maynard, Daniel S., additional, Renner, Susanne S., additional, van den Hoogen, Johan, additional, Zou, Yibiao, additional, Liang, Jingjing, additional, de-Miguel, Sergio, additional, Nabuurs, Gert-Jan, additional, Reich, Peter B., additional, Niinemets, Ülo, additional, Abegg, Meinrad, additional, Adou Yao, Yves C., additional, Alberti, Giorgio, additional, Almeyda Zambrano, Angelica M., additional, Alvarado, Braulio Vilchez, additional, Alvarez-Dávila, Esteban, additional, Alvarez-Loayza, Patricia, additional, Alves, Luciana F., additional, Ammer, Christian, additional, Antón-Fernández, Clara, additional, Araujo-Murakami, Alejandro, additional, Arroyo, Luzmila, additional, Avitabile, Valerio, additional, Aymard, Gerardo A., additional, Baker, Timothy R., additional, Bałazy, Radomir, additional, Banki, Olaf, additional, Barroso, Jorcely G., additional, Bastian, Meredith L., additional, Bastin, Jean-Francois, additional, Birigazzi, Luca, additional, Birnbaum, Philippe, additional, Bitariho, Robert, additional, Boeckx, Pascal, additional, Bongers, Frans, additional, Bouriaud, Olivier, additional, Brancalion, Pedro H. S., additional, Brandl, Susanne, additional, Brearley, Francis Q., additional, Brienen, Roel, additional, Broadbent, Eben N., additional, Bruelheide, Helge, additional, Bussotti, Filippo, additional, Cazzolla Gatti, Roberto, additional, César, Ricardo G., additional, Cesljar, Goran, additional, Chazdon, Robin, additional, Chen, Han Y. H., additional, Chisholm, Chelsea, additional, Cho, Hyunkook, additional, Cienciala, Emil, additional, Clark, Connie, additional, Clark, David, additional, Colletta, Gabriel D., additional, Coomes, David A., additional, Valverde, Fernando Cornejo, additional, Corral-Rivas, José J., additional, Crim, Philip M., additional, Cumming, Jonathan R., additional, Dayanandan, Selvadurai, additional, de Gasper, André L., additional, Decuyper, Mathieu, additional, Derroire, Géraldine, additional, DeVries, Ben, additional, Djordjevic, Ilija, additional, Dolezal, Jiri, additional, Dourdain, Aurélie, additional, Engone Obiang, Nestor Laurier, additional, Enquist, Brian J., additional, Eyre, Teresa J., additional, Fandohan, Adandé Belarmain, additional, Fayle, Tom M., additional, Feldpausch, Ted R., additional, Ferreira, Leandro V., additional, Finér, Leena, additional, Fischer, Markus, additional, Fletcher, Christine, additional, Fridman, Jonas, additional, Frizzera, Lorenzo, additional, Gamarra, Javier G. P., additional, Gianelle, Damiano, additional, Glick, Henry B., additional, Harris, David J., additional, Hector, Andrew, additional, Hemp, Andreas, additional, Hengeveld, Geerten, additional, Hérault, Bruno, additional, Herbohn, John L., additional, Herold, Martin, additional, Hillers, Annika, additional, Honorio Coronado, Eurídice N., additional, Hui, Cang, additional, Ibanez, Thomas T., additional, Amaral, Iêda, additional, Imai, Nobuo, additional, Jagodziński, Andrzej M., additional, Jaroszewicz, Bogdan, additional, Johannsen, Vivian Kvist, additional, Joly, Carlos A., additional, Jucker, Tommaso, additional, Jung, Ilbin, additional, Karminov, Viktor, additional, Kartawinata, Kuswata, additional, Kearsley, Elizabeth, additional, Kenfack, David, additional, Kennard, Deborah K., additional, Kepfer-Rojas, Sebastian, additional, Keppel, Gunnar, additional, Khan, Mohammed Latif, additional, Killeen, Timothy J., additional, Kim, Hyun Seok, additional, Kitayama, Kanehiro, additional, Köhl, Michael, additional, Korjus, Henn, additional, Kraxner, Florian, additional, Kucher, Dmitry, additional, Laarmann, Diana, additional, Lang, Mait, additional, Lewis, Simon L., additional, Lu, Huicui, additional, Lukina, Natalia V., additional, Maitner, Brian S., additional, Malhi, Yadvinder, additional, Marcon, Eric, additional, Marimon, Beatriz Schwantes, additional, Marimon-Junior, Ben Hur, additional, Marshall, Andrew R., additional, Martin, Emanuel H., additional, Meave, Jorge A., additional, Melo-Cruz, Omar, additional, Mendoza, Casimiro, additional, Merow, Cory, additional, Monteagudo Mendoza, Abel, additional, Moreno, Vanessa S., additional, Mukul, Sharif A., additional, Mundhenk, Philip, additional, Nava-Miranda, María Guadalupe, additional, Neill, David, additional, Neldner, Victor J., additional, Nevenic, Radovan V., additional, Ngugi, Michael R., additional, Niklaus, Pascal A., additional, Oleksyn, Jacek, additional, Ontikov, Petr, additional, Ortiz-Malavasi, Edgar, additional, Pan, Yude, additional, Paquette, Alain, additional, Parada-Gutierrez, Alexander, additional, Parfenova, Elena I., additional, Park, Minjee, additional, Parren, Marc, additional, Parthasarathy, Narayanaswamy, additional, Peri, Pablo L., additional, Pfautsch, Sebastian, additional, Phillips, Oliver L., additional, Picard, Nicolas, additional, Piedade, Maria Teresa F., additional, Piotto, Daniel, additional, Pitman, Nigel C. A., additional, Mendoza-Polo, Irina, additional, Poulsen, Axel D., additional, Poulsen, John R., additional, Pretzsch, Hans, additional, Ramirez Arevalo, Freddy, additional, Restrepo-Correa, Zorayda, additional, Rodeghiero, Mirco, additional, Rolim, Samir G., additional, Roopsind, Anand, additional, Rovero, Francesco, additional, Rutishauser, Ervan, additional, Saikia, Purabi, additional, Salas-Eljatib, Christian, additional, Saner, Philippe, additional, Schall, Peter, additional, Schelhaas, Mart-Jan, additional, Schepaschenko, Dmitry, additional, Scherer-Lorenzen, Michael, additional, Schmid, Bernhard, additional, Schöngart, Jochen, additional, Searle, Eric B., additional, Seben, Vladimír, additional, Serra-Diaz, Josep M., additional, Sheil, Douglas, additional, Shvidenko, Anatoly Z., additional, Silva-Espejo, Javier E., additional, Silveira, Marcos, additional, Singh, James, additional, Sist, Plinio, additional, Slik, Ferry, additional, Sonké, Bonaventure, additional, Souza, Alexandre F., additional, Miścicki, Stanislaw, additional, Stereńczak, Krzysztof J., additional, Svenning, Jens-Christian, additional, Svoboda, Miroslav, additional, Swanepoel, Ben, additional, Targhetta, Natalia, additional, Tchebakova, Nadja, additional, ter Steege, Hans, additional, Thomas, Raquel, additional, Tikhonova, Elena, additional, Umunay, Peter M., additional, Usoltsev, Vladimir A., additional, Valencia, Renato, additional, Valladares, Fernando, additional, van der Plas, Fons, additional, Van Do, Tran, additional, van Nuland, Michael E., additional, Vasquez, Rodolfo M., additional, Verbeeck, Hans, additional, Viana, Helder, additional, Vibrans, Alexander C., additional, Vieira, Simone, additional, von Gadow, Klaus, additional, Wang, Hua-Feng, additional, Watson, James V., additional, Werner, Gijsbert D. A., additional, Westerlund, Bertil, additional, Wiser, Susan K., additional, Wittmann, Florian, additional, Woell, Hannsjoerg, additional, Wortel, Verginia, additional, Zagt, Roderick, additional, Zawiła-Niedźwiecki, Tomasz, additional, Zhang, Chunyu, additional, Zhao, Xiuhai, additional, Zhou, Mo, additional, Zhu, Zhi-Xin, additional, Zo-Bi, Irie C., additional, and Zohner, Constantin M., additional
- Published
- 2023
- Full Text
- View/download PDF
111. Cost-Effectiveness of a Comprehensive Primary Care Smoking Treatment Program
- Author
-
Mundt, Marlon P., primary, McCarthy, Danielle E., additional, Baker, Timothy B., additional, Zehner, Mark E., additional, Zwaga, Deejay, additional, and Fiore, Michael C., additional
- Published
- 2023
- Full Text
- View/download PDF
112. Tobacco Use Prevalence and Smoking Cessation Pharmacotherapy Prescription Patterns Among Hospitalized Patients by Medical Specialty
- Author
-
Srivastava, A. Benjamin, Ramsey, Alex T., McIntosh, Leslie D., Bailey, Thomas C., Fisher, Sherri L., Fox, Louis, Castro, Mario, Ma, Yinjiao, Baker, Timothy B., Chen, Li-Shiun, and Bierut, Laura J.
- Published
- 2019
113. Dominant tree species drive beta diversity patterns in western Amazonia
- Author
-
Draper, Frederick C., Asner, Gregory P., Coronado, Eurídice N. Honorio, Baker, Timothy R., García-Villacorta, Roosevelt, Pitman, Nigel C. A., Fine, Paul V. A., Phillips, Oliver L., Gómez, Ricardo Zárate, Amasifuén Guerra, Carlos A., Arévalo, Manuel Flores, Martínez, Rodolfo Vásquez, Brienen, Roel J. W., Monteagudo-Mendoza, Abel, Montenegro, Luis A. Torres, Sandoval, Elvis Valderrama, Roucoux, Katherine H., Ramírez Arévalo, Fredy R., Acuy, Ítalo Mesones, Del Aguila Pasquel, Jhon, Casapia, Ximena Tagle, Llampazo, Gerardo Flores, Medina, Massiel Corrales, Huaymacari, José Reyna, and Baraloto, Christopher
- Published
- 2019
114. Fluorescent Protein-Tagged Sindbis Virus E2 Glycoprotein Allows Single Particle Analysis of Virus Budding from Live Cells.
- Author
-
Jose, Joyce, Tang, Jinghua, Taylor, Aaron B, Baker, Timothy S, and Kuhn, Richard J
- Subjects
Cell Line ,Animals ,Sindbis Virus ,Luminescent Proteins ,Recombinant Fusion Proteins ,Viral Envelope Proteins ,Staining and Labeling ,Virus Assembly ,Cricetinae ,Virus Internalization ,Virus Attachment ,Virus Release ,alphavirus ,filopodia ,live imaging ,virus assembly ,virus egress ,Microbiology - Abstract
Sindbis virus (SINV) is an enveloped, mosquito-borne alphavirus. Here we generated and characterized a fluorescent protein-tagged (FP-tagged) SINV and found that the presence of the FP-tag (mCherry) affected glycoprotein transport to the plasma membrane whereas the specific infectivity of the virus was not affected. We examined the virions by transmission electron cryo-microscopy and determined the arrangement of the FP-tag on the surface of the virion. The fluorescent proteins are arranged icosahedrally on the virus surface in a stable manner that did not adversely affect receptor binding or fusion functions of E2 and E1, respectively. The delay in surface expression of the viral glycoproteins, as demonstrated by flow cytometry analysis, contributed to a 10-fold reduction in mCherry-E2 virus titer. There is a 1:1 ratio of mCherry to E2 incorporated into the virion, which leads to a strong fluorescence signal and thus facilitates single-particle tracking experiments. We used the FP-tagged virus for high-resolution live-cell imaging to study the spatial and temporal aspects of alphavirus assembly and budding from mammalian cells. These processes were further analyzed by thin section microscopy. The results demonstrate that SINV buds from the plasma membrane of infected cells and is dispersed into the surrounding media or spread to neighboring cells facilitated by its close association with filopodial extensions.
- Published
- 2015
115. Bullying, School Climate, Social Climate and Intellectual Development: Implications for the Lives of High-Achieving, Creative Students
- Author
-
Hoover, John, Larson, Joanne M., and Baker, Timothy D.
- Abstract
This paper consists of a selective, critical literature review of research dealing with the potential relationship between bullying and the characteristics associated with potential status as creative, gifted, and talented. While no clear and direct indication exists that gifted or talented status produces risk for victimization, indirect evidence for such a relationship may be associated with three sets of mediating variables. First, we noted a more direct association between bullying and the intellectual and social climate within schools. Second, some bullying is probably motivated by the nature of gendered expectations, differentially impacting boys in literacy and fine arts and girls in mathematics and science. Finally, the individual level of social and linguistic skills appears to be associated with victimization. We propose a model for exploring bullying as a mediating variable between school climate issues and gifted-talented status and for investigating gender differences in manifestations of creativity and intellectual giftedness. In the proposed model, bullying serves as a mediating variable when considering the climate of the school and gender expectations on students' academic achievement as well as on the Creative, Gifted, and Talented (CGT) status and the quality of school life for these individuals.
- Published
- 2013
116. The Ghost of 'Emo:' Searching for Mental Health Themes in a Popular Music Format
- Author
-
Baker, Timothy D., Smith-Adcock, Sondra, and Glynn, Virginia R.
- Abstract
The concept of "Emo" has gained attention among counselors who work with teens in school settings. Emo has been associated with music and popular media has linked it to mental health concerns, but scholarly sources have not converged regarding what sort of music it is, or what it means for adolescents' wellness. The authors devise and explain a procedure for identifying and analyzing music with Emo characteristics. Several songs were identified having lyrics or video imagery that portrayed mental health scenarios, sufficient to trigger counselors' duty-to-warn and/or mandated reporting obligations. Recommendations are made for the practice of school counselors. (Contains 1 table.)
- Published
- 2013
117. Author Correction: Tree mode of death and mortality risk factors across Amazon forests
- Author
-
Esquivel-Muelbert, Adriane, Phillips, Oliver L., Brienen, Roel J. W., Fauset, Sophie, Sullivan, Martin J. P., Baker, Timothy R., Chao, Kuo-Jung, Feldpausch, Ted R., Gloor, Emanuel, Higuchi, Niro, Houwing-Duistermaat, Jeanne, Lloyd, Jon, Liu, Haiyan, Malhi, Yadvinder, Marimon, Beatriz, Marimon Junior, Ben Hur, Monteagudo-Mendoza, Abel, Poorter, Lourens, Silveira, Marcos, Torre, Emilio Vilanova, Dávila, Esteban Alvarez, del Aguila Pasquel, Jhon, Almeida, Everton, Loayza, Patricia Alvarez, Andrade, Ana, Aragão, Luiz E. O. C., Araujo-Murakami, Alejandro, Arets, Eric, Arroyo, Luzmila, Aymard C., Gerardo A., Baisie, Michel, Baraloto, Christopher, Camargo, Plínio Barbosa, Barroso, Jorcely, Blanc, Lilian, Bonal, Damien, Bongers, Frans, Boot, René, Brown, Foster, Burban, Benoit, Camargo, José Luís, Castro, Wendeson, Moscoso, Victor Chama, Chave, Jerome, Comiskey, James, Valverde, Fernando Cornejo, da Costa, Antonio Lola, Cardozo, Nallaret Davila, Di Fiore, Anthony, Dourdain, Aurélie, Erwin, Terry, Llampazo, Gerardo Flores, Vieira, Ima Célia Guimarães, Herrera, Rafael, Honorio Coronado, Eurídice, Huamantupa-Chuquimaco, Isau, Jimenez-Rojas, Eliana, Killeen, Timothy, Laurance, Susan, Laurance, William, Levesley, Aurora, Lewis, Simon L., Ladvocat, Karina Liana Lisboa Melgaço, Lopez-Gonzalez, Gabriela, Lovejoy, Thomas, Meir, Patrick, Mendoza, Casimiro, Morandi, Paulo, Neill, David, Nogueira Lima, Adriano José, Vargas, Percy Nuñez, de Oliveira, Edmar Almeida, Camacho, Nadir Pallqui, Pardo, Guido, Peacock, Julie, Peña-Claros, Marielos, Peñuela-Mora, Maria Cristina, Pickavance, Georgia, Pipoly, John, Pitman, Nigel, Prieto, Adriana, Pugh, Thomas A. M., Quesada, Carlos, Ramirez-Angulo, Hirma, de Almeida Reis, Simone Matias, Rejou-Machain, Maxime, Correa, Zorayda Restrepo, Bayona, Lily Rodriguez, Rudas, Agustín, Salomão, Rafael, Serrano, Julio, Espejo, Javier Silva, Silva, Natalino, Singh, James, Stahl, Clement, Stropp, Juliana, Swamy, Varun, Talbot, Joey, ter Steege, Hans, Terborgh, John, Thomas, Raquel, Toledo, Marisol, Torres-Lezama, Armando, Gamarra, Luis Valenzuela, van der Heijden, Geertje, van der Meer, Peter, van der Hout, Peter, Martinez, Rodolfo Vasquez, Vieira, Simone Aparecida, Cayo, Jeanneth Villalobos, Vos, Vincent, Zagt, Roderick, Zuidema, Pieter, and Galbraith, David
- Published
- 2021
- Full Text
- View/download PDF
118. Non-structural carbohydrates mediate seasonal water stress across Amazon forests
- Author
-
Signori-Müller, Caroline, Oliveira, Rafael S., Barros, Fernanda de Vasconcellos, Tavares, Julia Valentim, Gilpin, Martin, Diniz, Francisco Carvalho, Zevallos, Manuel J. Marca, Yupayccana, Carlos A. Salas, Acosta, Martin, Bacca, Jean, Chino, Rudi S. Cruz, Cuellar, Gina M. Aramayo, Cumapa, Edwin R. M., Martinez, Franklin, Mullisaca, Flor M. Pérez, Nina, Alex, Sanchez, Jesus M. Bañon, da Silva, Leticia Fernandes, Tello, Ligia, Tintaya, José Sanchez, Ugarteche, Maira T. Martinez, Baker, Timothy R., Bittencourt, Paulo R. L., Borma, Laura S., Brum, Mauro, Castro, Wendeson, Coronado, Eurídice N. Honorio, Cosio, Eric G., Feldpausch, Ted R., Fonseca, Letícia d’Agosto Miguel, Gloor, Emanuel, Llampazo, Gerardo Flores, Malhi, Yadvinder, Mendoza, Abel Monteagudo, Moscoso, Victor Chama, Araujo-Murakami, Alejandro, Phillips, Oliver L., Salinas, Norma, Silveira, Marcos, Talbot, Joey, Vasquez, Rodolfo, Mencuccini, Maurizio, and Galbraith, David
- Published
- 2021
- Full Text
- View/download PDF
119. Predictors of adherence to nicotine replacement therapy: Machine learning evidence that perceived need predicts medication use
- Author
-
Kim, Nayoung, McCarthy, Danielle E., Loh, Wei-Yin, Cook, Jessica W., Piper, Megan E., Schlam, Tanya R., and Baker, Timothy B.
- Published
- 2019
- Full Text
- View/download PDF
120. Underutilization of Guideline-Concordant Smoking Cessation Treatments in Surgical Patients: Lessons From a Learning Health System
- Author
-
Heiden, Brendan T., Smock, Nina, Pham, Giang, Chen, Jingling, Craig, Ethan J., Meyers, Bryan F., Puri, Varun, Colditz, Graham A, Baker, Timothy B., Bierut, Laura J., Kozower, Benjamin D., and Chen, Li-Shiun
- Published
- 2022
- Full Text
- View/download PDF
121. Asynchronous carbon sink saturation in African and Amazonian tropical forests
- Author
-
Hubau, Wannes, Lewis, Simon L., Phillips, Oliver L., Affum-Baffoe, Kofi, Beeckman, Hans, Cuní-Sanchez, Aida, Daniels, Armandu K., Ewango, Corneille E. N., Fauset, Sophie, Mukinzi, Jacques M., Sheil, Douglas, Sonké, Bonaventure, Sullivan, Martin J. P., Sunderland, Terry C. H., Taedoumg, Hermann, Thomas, Sean C., White, Lee J. T., Abernethy, Katharine A., Adu-Bredu, Stephen, Amani, Christian A., Baker, Timothy R., Banin, Lindsay F., Baya, Fidèle, Begne, Serge K., Bennett, Amy C., Benedet, Fabrice, Bitariho, Robert, Bocko, Yannick E., Boeckx, Pascal, Boundja, Patrick, Brienen, Roel J. W., Brncic, Terry, Chezeaux, Eric, Chuyong, George B., Clark, Connie J., Collins, Murray, Comiskey, James A., Coomes, David A., Dargie, Greta C., de Haulleville, Thales, Kamdem, Marie Noel Djuikouo, Doucet, Jean-Louis, Esquivel-Muelbert, Adriane, Feldpausch, Ted R., Fofanah, Alusine, Foli, Ernest G., Gilpin, Martin, Gloor, Emanuel, Gonmadje, Christelle, Gourlet-Fleury, Sylvie, Hall, Jefferson S., Hamilton, Alan C., Harris, David J., Hart, Terese B., Hockemba, Mireille B. N., Hladik, Annette, Ifo, Suspense A., Jeffery, Kathryn J., Jucker, Tommaso, Yakusu, Emmanuel Kasongo, Kearsley, Elizabeth, Kenfack, David, Koch, Alexander, Leal, Miguel E., Levesley, Aurora, Lindsell, Jeremy A., Lisingo, Janvier, Lopez-Gonzalez, Gabriela, Lovett, Jon C., Makana, Jean-Remy, Malhi, Yadvinder, Marshall, Andrew R., Martin, Jim, Martin, Emanuel H., Mbayu, Faustin M., Medjibe, Vincent P., Mihindou, Vianet, Mitchard, Edward T. A., Moore, Sam, Munishi, Pantaleo K. T., Bengone, Natacha Nssi, Ojo, Lucas, Ondo, Fidèle Evouna, Peh, Kelvin S.-H., Pickavance, Georgia C., Poulsen, Axel Dalberg, Poulsen, John R., Qie, Lan, Reitsma, Jan, Rovero, Francesco, Swaine, Michael D., Talbot, Joey, Taplin, James, Taylor, David M., Thomas, Duncan W., Toirambe, Benjamin, Mukendi, John Tshibamba, Tuagben, Darlington, Umunay, Peter M., van der Heijden, Geertje M. F., Verbeeck, Hans, Vleminckx, Jason, Willcock, Simon, Wöll, Hannsjörg, Woods, John T., and Zemagho, Lise
- Published
- 2020
- Full Text
- View/download PDF
122. CHRNA5 Risk Variant Predicts Delayed Smoking Cessation and Earlier Lung Cancer Diagnosis—A Meta-Analysis
- Author
-
Chen, Li-Shiun, Hung, Rayjean J, Baker, Timothy, Horton, Amy, Culverhouse, Rob, Saccone, Nancy, Cheng, Iona, Deng, Bo, Han, Younghun, Hansen, Helen M, Horsman, Janet, Kim, Claire, Lutz, Sharon, Rosenberger, Albert, Aben, Katja K, Andrew, Angeline S, Breslau, Naomi, Chang, Shen-Chih, Dieffenbach, Aida Karina, Dienemann, Hendrik, Frederiksen, Brittni, Han, Jiali, Hatsukami, Dorothy K, Johnson, Eric O, Pande, Mala, Wrensch, Margaret R, McLaughlin, John, Skaug, Vidar, van der Heijden, Henricus F, Wampfler, Jason, Wenzlaff, Angela, Woll, Penella, Zienolddiny, Shanbeh, Bickeböller, Heike, Brenner, Hermann, Duell, Eric J, Haugen, Aage, Heinrich, Joachim, Hokanson, John E, Hunter, David J, Kiemeney, Lambertus A, Lazarus, Philip, Le Marchand, Loic, Liu, Geoffrey, Mayordomo, Jose, Risch, Angela, Schwartz, Ann G, Teare, Dawn, Wu, Xifeng, Wiencke, John K, Yang, Ping, Zhang, Zuo-Feng, Spitz, Margaret R, Kraft, Peter, Amos, Christopher I, and Bierut, Laura J
- Subjects
Biomedical and Clinical Sciences ,Oncology and Carcinogenesis ,Clinical Research ,Lung ,Lung Cancer ,Cancer ,Human Genome ,Tobacco ,Prevention ,Genetics ,Tobacco Smoke and Health ,Detection ,screening and diagnosis ,4.2 Evaluation of markers and technologies ,Respiratory ,Good Health and Well Being ,Genetic Variation ,Humans ,Lung Neoplasms ,Middle Aged ,Nerve Tissue Proteins ,Phenotype ,Receptors ,Nicotinic ,Risk Factors ,Smoking ,Smoking Cessation ,Time Factors ,Oncology & Carcinogenesis ,Oncology and carcinogenesis - Abstract
BackgroundRecent meta-analyses show strong evidence of associations among genetic variants in CHRNA5 on chromosome 15q25, smoking quantity, and lung cancer. This meta-analysis tests whether the CHRNA5 variant rs16969968 predicts age of smoking cessation and age of lung cancer diagnosis.MethodsMeta-analyses examined associations between rs16969968, age of quitting smoking, and age of lung cancer diagnosis in 24 studies of European ancestry (n = 29 072). In each dataset, we used Cox regression models to evaluate the association between rs16969968 and the two primary phenotypes (age of smoking cessation among ever smokers and age of lung cancer diagnosis among lung cancer case patients) and the secondary phenotype of smoking duration. Heterogeneity across studies was assessed with the Cochran Q test. All statistical tests were two-sided.ResultsThe rs16969968 allele (A) was associated with a lower likelihood of smoking cessation (hazard ratio [HR] = 0.95, 95% confidence interval [CI] = 0.91 to 0.98, P = .0042), and the AA genotype was associated with a four-year delay in median age of quitting compared with the GG genotype. Among smokers with lung cancer diagnoses, the rs16969968 genotype (AA) was associated with a four-year earlier median age of diagnosis compared with the low-risk genotype (GG) (HR = 1.08, 95% CI = 1.04 to 1.12, P = 1.1*10(-5)).ConclusionThese data support the clinical significance of the CHRNA5 variant rs16969968. It predicts delayed smoking cessation and an earlier age of lung cancer diagnosis in this meta-analysis. Given the existing evidence that this CHRNA5 variant predicts favorable response to cessation pharmacotherapy, these findings underscore the potential clinical and public health importance of rs16969968 in CHRNA5 in relation to smoking cessation success and lung cancer risk.
- Published
- 2015
123. Adeno-Associated Virus Serotype 1 (AAV1)- and AAV5-Antibody Complex Structures Reveal Evolutionary Commonalities in Parvovirus Antigenic Reactivity
- Author
-
Tseng, Yu-Shan, Gurda, Brittney L, Chipman, Paul, McKenna, Robert, Afione, Sandra, Chiorini, John A, Muzyczka, Nicholas, Olson, Norman H, Baker, Timothy S, Kleinschmidt, Jürgen, and Agbandje-McKenna, Mavis
- Subjects
Genetics ,Biotechnology ,Gene Therapy ,Infection ,Good Health and Well Being ,Antibodies ,Monoclonal ,Antibodies ,Neutralizing ,Antibodies ,Viral ,Cryoelectron Microscopy ,Dependovirus ,Epitope Mapping ,Epitopes ,Humans ,Image Processing ,Computer-Assisted ,Macromolecular Substances ,Models ,Molecular ,Protein Binding ,Serogroup ,Biological Sciences ,Agricultural and Veterinary Sciences ,Medical and Health Sciences ,Virology - Abstract
UnlabelledThe clinical utility of the adeno-associated virus (AAV) gene delivery system has been validated by the regulatory approval of an AAV serotype 1 (AAV1) vector for the treatment of lipoprotein lipase deficiency. However, neutralization from preexisting antibodies is detrimental to AAV transduction efficiency. Hence, mapping of AAV antigenic sites and engineering of neutralization-escaping vectors are important for improving clinical efficacy. We report the structures of four AAV-monoclonal antibody fragment complexes, AAV1-ADK1a, AAV1-ADK1b, AAV5-ADK5a, and AAV5-ADK5b, determined by cryo-electron microscopy and image reconstruction to a resolution of ∼11 to 12 Å. Pseudoatomic modeling mapped the ADK1a epitope to the protrusions surrounding the icosahedral 3-fold axis and the ADK1b and ADK5a epitopes, which overlap, to the wall between depressions at the 2- and 5-fold axes (2/5-fold wall), and the ADK5b epitope spans both the 5-fold axis-facing wall of the 3-fold protrusion and portions of the 2/5-fold wall of the capsid. Combined with the six antigenic sites previously elucidated for different AAV serotypes through structural approaches, including AAV1 and AAV5, this study identified two common AAV epitopes: one on the 3-fold protrusions and one on the 2/5-fold wall. These epitopes coincide with regions with the highest sequence and structure diversity between AAV serotypes and correspond to regions determining receptor recognition and transduction phenotypes. Significantly, these locations overlap the two dominant epitopes reported for autonomous parvoviruses. Thus, rather than the amino acid sequence alone, the antigenic sites of parvoviruses appear to be dictated by structural features evolved to enable specific infectious functions.ImportanceThe adeno-associated viruses (AAVs) are promising vectors for in vivo therapeutic gene delivery, with more than 20 years of intense research now realized in a number of successful human clinical trials that report therapeutic efficacy. However, a large percentage of the population has preexisting AAV capsid antibodies and therefore must be excluded from clinical trials or vector readministration. This report represents our continuing efforts to understand the antigenic structure of the AAVs, specifically, to obtain a picture of "polyclonal" reactivity as is the situation in humans. It describes the structures of four AAV-antibody complexes determined by cryo-electron microscopy and image reconstruction, increasing the number of mapped epitopes to four and three, respectively, for AAV1 and AAV5, two vectors currently in clinical trials. The results presented provide information essential for generating antigenic escape vectors to overcome a critical challenge remaining in the optimization of this highly promising vector delivery system.
- Published
- 2015
124. Three-Dimensional Structure of a Protozoal Double-Stranded RNA Virus That Infects the Enteric Pathogen Giardia lamblia
- Author
-
Janssen, Mandy EW, Takagi, Yuko, Parent, Kristin N, Cardone, Giovanni, Nibert, Max L, and Baker, Timothy S
- Subjects
Emerging Infectious Diseases ,Digestive Diseases ,Biodefense ,Vaccine Related ,Foodborne Illness ,Infectious Diseases ,Prevention ,Aetiology ,2.2 Factors relating to the physical environment ,Infection ,Good Health and Well Being ,Cryoelectron Microscopy ,Giardia lamblia ,Giardiavirus ,Imaging ,Three-Dimensional ,Virion ,Biological Sciences ,Agricultural and Veterinary Sciences ,Medical and Health Sciences ,Virology - Abstract
UnlabelledGiardia lamblia virus (GLV) is a small, nonenveloped, nonsegmented double-stranded RNA (dsRNA) virus infecting Giardia lamblia, the most common protozoan pathogen of the human intestine and a major agent of waterborne diarrheal disease worldwide. GLV (genus Giardiavirus) is a member of family Totiviridae, along with several other groups of protozoal or fungal viruses, including Leishmania RNA viruses and Trichomonas vaginalis viruses. Interestingly, GLV is more closely related than other Totiviridae members to a group of recently discovered metazoan viruses that includes penaeid shrimp infectious myonecrosis virus (IMNV). Moreover, GLV is the only known protozoal dsRNA virus that can transmit efficiently by extracellular means, also like IMNV. In this study, we used transmission electron cryomicroscopy and icosahedral image reconstruction to examine the GLV virion at an estimated resolution of 6.0 Å. Its outermost diameter is 485 Å, making it the largest totivirus capsid analyzed to date. Structural comparisons of GLV and other totiviruses highlighted a related "T=2" capsid organization and a conserved helix-rich fold in the capsid subunits. In agreement with its unique capacity as a protozoal dsRNA virus to survive and transmit through extracellular environments, GLV was found to be more thermoresistant than Trichomonas vaginalis virus 1, but no specific protein machinery to mediate cell entry, such as the fiber complexes in IMNV, could be localized. These and other structural and biochemical findings provide a basis for future work to dissect the cell entry mechanism of GLV into a "primitive" (early-branching) eukaryotic host and an important enteric pathogen of humans.ImportanceNumerous pathogenic bacteria, including Corynebacterium diphtheriae, Salmonella enterica, and Vibrio cholerae, are infected with lysogenic bacteriophages that contribute significantly to bacterial virulence. In line with this phenomenon, several pathogenic protozoa, including Giardia lamblia, Leishmania species, and Trichomonas vaginalis are persistently infected with dsRNA viruses, and growing evidence indicates that at least some of these protozoal viruses can likewise enhance the pathogenicity of their hosts. Understanding of these protozoal viruses, however, lags far behind that of many bacteriophages. Here, we investigated the dsRNA virus that infects the widespread enteric parasite Giardia lamblia. Using electron cryomicroscopy and icosahedral image reconstruction, we determined the virion structure of Giardia lamblia virus, obtaining new information relating to its assembly, stability, functions in cell entry and transcription, and similarities and differences with other dsRNA viruses. The results of our study set the stage for further mechanistic work on the roles of these viruses in protozoal virulence.
- Published
- 2015
125. Drug Metabolizing Enzyme and Transporter Gene Variation, Nicotine Metabolism, Prospective Abstinence, and Cigarette Consumption
- Author
-
Bergen, Andrew W, Michel, Martha, Nishita, Denise, Krasnow, Ruth, Javitz, Harold S, Conneely, Karen N, Lessov-Schlaggar, Christina N, Hops, Hyman, Zhu, Andy ZX, Baurley, James W, McClure, Jennifer B, Hall, Sharon M, Baker, Timothy B, Conti, David V, Benowitz, Neal L, Lerman, Caryn, Tyndale, Rachel F, and Swan, Gary E
- Subjects
Biological Sciences ,Biomedical and Clinical Sciences ,Genetics ,Biological Psychology ,Psychology ,Pharmacology and Pharmaceutical Sciences ,Substance Misuse ,Tobacco ,Drug Abuse (NIDA only) ,Lung ,Cancer ,Clinical Trials and Supportive Activities ,Tobacco Smoke and Health ,Clinical Research ,Lung Cancer ,Prevention ,Good Health and Well Being ,Adult ,Alleles ,Cytochrome P-450 CYP2A6 ,Female ,Genetic Association Studies ,Genetic Variation ,Humans ,Male ,Middle Aged ,Nicotine ,Polymorphism ,Single Nucleotide ,Risk Factors ,Smoking ,Smoking Cessation ,Transdisciplinary Research in Cancer of the Lung Research Team ,General Science & Technology - Abstract
The Nicotine Metabolite Ratio (NMR, ratio of trans-3'-hydroxycotinine and cotinine), has previously been associated with CYP2A6 activity, response to smoking cessation treatments, and cigarette consumption. We searched for drug metabolizing enzyme and transporter (DMET) gene variation associated with the NMR and prospective abstinence in 2,946 participants of laboratory studies of nicotine metabolism and of clinical trials of smoking cessation therapies. Stage I was a meta-analysis of the association of 507 common single nucleotide polymorphisms (SNPs) at 173 DMET genes with the NMR in 449 participants of two laboratory studies. Nominally significant associations were identified in ten genes after adjustment for intragenic SNPs; CYP2A6 and two CYP2A6 SNPs attained experiment-wide significance adjusted for correlated SNPs (CYP2A6 PACT=4.1E-7, rs4803381 PACT=4.5E-5, rs1137115, PACT=1.2E-3). Stage II was mega-regression analyses of 10 DMET SNPs with pretreatment NMR and prospective abstinence in up to 2,497 participants from eight trials. rs4803381 and rs1137115 SNPs were associated with pretreatment NMR at genome-wide significance. In post-hoc analyses of CYP2A6 SNPs, we observed nominally significant association with: abstinence in one pharmacotherapy arm; cigarette consumption among all trial participants; and lung cancer in four case:control studies. CYP2A6 minor alleles were associated with reduced NMR, CPD, and lung cancer risk. We confirmed the major role that CYP2A6 plays in nicotine metabolism, and made novel findings with respect to genome-wide significance and associations with CPD, abstinence and lung cancer risk. Additional multivariate analyses with patient variables and genetic modeling will improve prediction of nicotine metabolism, disease risk and smoking cessation treatment prognosis.
- Published
- 2015
126. Evolutionary diversity is associated with wood productivity in Amazonian forests
- Author
-
Coelho de Souza, Fernanda, Dexter, Kyle G., Phillips, Oliver L., Pennington, R. Toby, Neves, Danilo, Sullivan, Martin J. P., Alvarez-Davila, Esteban, Alves, Átila, Amaral, Ieda, Andrade, Ana, Aragao, Luis E. O. C., Araujo-Murakami, Alejandro, Arets, Eric J. M. M., Arroyo, Luzmilla, Aymard C., Gerardo A., Bánki, Olaf, Baraloto, Christopher, Barroso, Jorcely G., Boot, Rene G. A., Brienen, Roel J. W., Brown, Foster, Camargo, José Luís C., Castro, Wendeson, Chave, Jerome, Cogollo, Alvaro, Comiskey, James A., Cornejo-Valverde, Fernando, da Costa, Antonio Lola, de Camargo, Plínio B., Di Fiore, Anthony, Feldpausch, Ted R., Galbraith, David R., Gloor, Emanuel, Goodman, Rosa C., Gilpin, Martin, Herrera, Rafael, Higuchi, Niro, Honorio Coronado, Eurídice N., Jimenez-Rojas, Eliana, Killeen, Timothy J., Laurance, Susan, Laurance, William F., Lopez-Gonzalez, Gabriela, Lovejoy, Thomas E., Malhi, Yadvinder, Marimon, Beatriz S., Marimon-Junior, Ben Hur, Mendoza, Casimiro, Monteagudo-Mendoza, Abel, Neill, David A., Vargas, Percy Núñez, Peñuela Mora, Maria C., Pickavance, Georgia C., Pipoly, III, John J., Pitman, Nigel C. A., Poorter, Lourens, Prieto, Adriana, Ramirez, Freddy, Roopsind, Anand, Rudas, Agustin, Salomão, Rafael P., Silva, Natalino, Silveira, Marcos, Singh, James, Stropp, Juliana, ter Steege, Hans, Terborgh, John, Thomas-Caesar, Raquel, Umetsu, Ricardo K., Vasquez, Rodolfo V., Célia-Vieira, Ima, Vieira, Simone A., Vos, Vincent A., Zagt, Roderick J., and Baker, Timothy R.
- Published
- 2019
- Full Text
- View/download PDF
127. Organic Cation Transporter Variation and Response to Smoking Cessation Therapies
- Author
-
Bergen, Andrew W, Javitz, Harold S, Krasnow, Ruth, Michel, Martha, Nishita, Denise, Conti, David V, Edlund, Christopher K, Kwok, Pui-Yan, McClure, Jennifer B, Kim, Richard B, Hall, Sharon M, Tyndale, Rachel F, Baker, Timothy B, Benowitz, Neal L, and Swan, Gary E
- Subjects
Epidemiology ,Public Health ,Health Sciences ,Substance Misuse ,Prevention ,Tobacco Smoke and Health ,Clinical Research ,Genetics ,Tobacco ,Clinical Trials and Supportive Activities ,6.1 Pharmaceuticals ,Evaluation of treatments and therapeutic interventions ,Cancer ,Good Health and Well Being ,Adult ,Benzazepines ,Female ,Genetic Variation ,Humans ,Male ,Middle Aged ,Organic Cation Transport Proteins ,Organic Cation Transporter 2 ,Polymorphism ,Single Nucleotide ,Prospective Studies ,Quinoxalines ,Smoking ,Smoking Cessation ,Tobacco Use Disorder ,Varenicline ,Clinical Sciences ,Public Health and Health Services ,Marketing ,Public health - Abstract
IntroductionWe evaluated chr6q25.3 organic cation transporter gene (SLC22A1, SLC22A2, SLC22A3) variation and response to smoking cessation therapies. The corresponding proteins are low-affinity transporters of choline, acetylcholine and monoamines, and smoking cessation pharmacotherapies expressed in multiple tissues.MethodsWe selected 7 common polymorphisms for mega-regression analysis. We assessed additive model association of polymorphisms with 7-day point prevalence abstinence overall and by assigned pharmacotherapy at end of treatment and at 6 months among European-ancestry participants of 7 randomized controlled trials adjusted for demographic, population genetic, and trial covariates.ResultsInitial results were obtained in 6 trials with 1,839 participants. Nominally statistically significant associations of 2 SLC22A2 polymorphisms were observed: (1) with rs316019 at 6 months, overall ([c.808T>G; p.Ser270Ala], OR = 1.306, 95% CI = 1.034-1.649, p = .025), and among those randomized to nicotine replacement therapy (NRT) (OR = 1.784, 95% CI = 1.072-2.970, p = .026); and (2) with rs316006 (c.1502-529A>T) among those randomized to varenicline (OR = 1.420, 95% CI = 1.038-1.944, p = .028, OR = 1.362, 95% CI = 1.001-1.853, p = .04) at end of treatment and 6 months. Individuals randomized to NRT from a seventh trial were genotyped for rs316019; rs316019 was associated with a nominally statistically significant effect on abstinence overall at 6 months among 2,233 participants (OR = 1.249, 95% CI = 1.007-1.550, p = .043).ConclusionsThe functional OCT2 Ser270Ala polymorphism is nominally statistically significantly associated with abstinence among European-ancestry treatment-seeking smokers after adjustments for pharmacotherapy, demographics, population genetics, and without adjustment for multiple testing of 7 SNPs. Replication of these preliminary findings in additional randomized controlled trials of smoking cessation therapies and from multiple continental populations would describe another pharmacogenetic role for SLC22A2/OCT2.
- Published
- 2014
128. A Deeper Look at Implicit Weight Bias in Medical Students
- Author
-
Baker, Timothy K., Smith, Gregory S., Jacobs, Negar Nicole, Houmanfar, Ramona, Tolles, Robbyn, Kuhls, Deborah, and Piasecki, Melissa
- Abstract
The Implicit Relational Assessment Procedure (IRAP, Barnes-Holmes et al. in Psychol Rec 60:527-542, 2010) was utilized as a relatively new tool to measure implicit weight bias in first- and third-year medical students. To date, only two studies (Miller et al. in Acad Med 88:978-982, 2013; Phelan et al. in Med Educ 49:983-992, 2015) have investigated implicit weight bias with medical students and both have found pro-thin/anti-fat implicit attitudes, on average, using the Implicit Association Test (IAT, Greenwald and Banaji in Psychol Rev 102:4-27, 1995) as the assessment tool. The IRAP, however, allows for a deeper analysis of implicit attitudes with respect to both thin and fat in isolation, and it was found that medical students are, on average, actually both pro-thin and pro-fat, and on average are more pro-thin than pro-fat, as opposed to anti-fat. Additionally, it was found that medical students' implicit weight bias against fat/obese individuals improved over the first 2 years of medical training, and this improvement was specifically driven by improved implicit attitudes toward overweight and obese, while implicit attitudes toward thin remained constant over that time. The implications of more sensitive implicit bias assessment and specific changes in bias over time are discussed within the context of medical education curriculum development.
- Published
- 2017
- Full Text
- View/download PDF
129. Effects of motivation phase intervention components on quit attempts in smokers unwilling to quit: A factorial experiment
- Author
-
Engle, Jessica L., Mermelstein, Robin, Baker, Timothy B., Smith, Stevens S., Schlam, Tanya R., Piper, Megan E., Jorenby, Douglas E., Collins, Linda M., and Cook, Jessica W.
- Published
- 2019
- Full Text
- View/download PDF
130. Paying Low-Income Smokers to Quit? The Cost-Effectiveness of Incentivizing Tobacco Quit Line Engagement for Medicaid Recipients Who Smoke
- Author
-
Mundt, Marlon P., Baker, Timothy B., Fraser, David L., Smith, Stevens S., Piper, Megan E., and Fiore, Michael C.
- Published
- 2019
- Full Text
- View/download PDF
131. The dynamics of the cold conveyor belt and sting jet in an extratropical cyclone and the influence of diabatic processes
- Author
-
Baker, Timothy Peter, Parker, Doug, Blyth, Alan, and Knippertz, Peter
- Subjects
551.55 - Abstract
This thesis examines the influences on the development of high winds in a case of a damaging extratropical cyclone. Extratropical cyclones are a regular occurrence over the United Kingdom (UK) and much of western Europe: strong cyclones can cause major damage and disruption through high winds, flooding and wave damage. Cyclone Friedhelm occurred on the 7th-8th December 2011, and was observed as part of the "DIAbatic Influence on Mesoscale features in ExTratropical Storms" (DIAMET) field campaign. A case of explosive cyclogenesis, Cyclone Friedhelm exhibited a deepening of 44 hPa in 24 hours, almost double that necessary to be considered a meteorological "bomb". As the storm passed over the UK, strong winds caused widespread damage and disruption, particularly across Scotland. These high winds and their influences are the focus of this thesis, in particular the high-wind region to the south-west of the cyclone centre, in which two high wind phenomena can occur, the cold-conveyor belt (CCB) and sting jets (SJ). The Weather Research and Forecasting (WRF) model is used with a high-resolution setup to investigate the mesoscale wind features, while a lower-resolution configuration is employed to examine the large-scale storm development. The high-resolution simulation is compared to routine observations, and those made specifically as part of the DIAMET project. The WRF model is shown to recreate the storm with a good degree of accuracy. The dynamics of the high wind regions are investigated in order to identify the features and the processes that contribute to their evolution. Trajectories are used to identify parcel ensembles resembling both the SJ and CCB. The effects of forces and balanced flows are discussed and a novel way to calculate the gradient flow is presented. The results of this analysis show a systematic region of super-gradient flow to the south and south-west of the cyclone centre and a region of sub-gradient flow to the north. The SJ parcels are analysed in relation to the past literature including a number of different theories on the cause of the SJ’s descent. Unlike some theories on SJs, latent cooling is shown to have little effect on the wind field, although it does change trajectory evolution. The lower-resolution setup shows that latent heating is key to the deepening of the cyclone, but little change in the track is observed when it is removed. The low-level PV anomaly is shown to be key in driving the deepening of the storm. Varying the time during the simulation at which latent heating is switched off shows the importance of the cyclone’s deepening rate on the wind speed development. Other novel analyses are conducted in order to examine the relationships between circulation and the high winds, showing a strong relationship between the circulation energy and cyclone pressure.
- Published
- 2014
132. Cost-effectiveness analysis of short versus long cephalomedullary nails for treatment of stable intertrochanteric femoral fractures: a theoretical cohort study
- Author
-
Fuller, Carson C., Kweon, Christopher, Baker, Timothy, Reese, Carlie, and Lack, William D.
- Published
- 2021
- Full Text
- View/download PDF
133. Cost-effectiveness of umeclidinium as add-on to ICS/LABA therapy in COPD: A UK perspective
- Author
-
Driessen, Maurice, Shah, Dhvani, Risebrough, Nancy, Baker, Timothy, Naya, Ian, Briggs, Andrew, and Ismaila, Afisi S.
- Published
- 2018
- Full Text
- View/download PDF
134. A novel partitivirus that confers hypovirulence on plant pathogenic fungi.
- Author
-
Xiao, Xueqiong, Cheng, Jiasen, Tang, Jinghua, Fu, Yanping, Jiang, Daohong, Baker, Timothy S, Ghabrial, Said A, and Xie, Jiatao
- Subjects
RNA Viruses ,Virion ,Ascomycota ,Mycelium ,Botrytis ,Arabidopsis ,Soybeans ,Lycopersicon esculentum ,RNA ,Viral ,Cryoelectron Microscopy ,Sequence Analysis ,DNA ,Virulence ,Molecular Sequence Data ,RNA ,Viral ,Sequence Analysis ,DNA ,Virology ,Biological Sciences ,Agricultural and Veterinary Sciences ,Medical and Health Sciences - Abstract
UnlabelledMembers of the family Partitiviridae have bisegmented double-stranded RNA (dsRNA) genomes and are not generally known to cause obvious symptoms in their natural hosts. An unusual partitivirus, Sclerotinia sclerotiorum partitivirus 1 (SsPV1/WF-1), conferred hypovirulence on its natural plant-pathogenic fungal host, Sclerotinia sclerotiorum strain WF-1. Cellular organelles, including mitochondria, were severely damaged. Hypovirulence and associated traits of strain WF-1 and SsPV1/WF-1 were readily cotransmitted horizontally via hyphal contact to different vegetative compatibility groups of S. sclerotiorum and interspecifically to Sclerotinia nivalis and Sclerotinia minor. S. sclerotiorum strain 1980 transfected with purified SsPV1/WF-1 virions also exhibited hypovirulence and associated traits similar to those of strain WF-1. Moreover, introduction of purified SsPV1/WF-1 virions into strain KY-1 of Botrytis cinerea also resulted in reductions in virulence and mycelial growth and, unexpectedly, enhanced conidial production. However, virus infection suppressed hyphal growth of most germinating conidia of B. cinerea and was eventually lethal to infected hyphae, since very few new colonies could develop following germ tube formation. Taken together, our results support the conclusion that SsPV1/WF-1 causes hypovirulence in Sclerotinia spp. and B. cinerea. Cryo-EM (cryo-electron microscopy) reconstruction of the SsPV1 particle shows that it has a distinct structure with similarity to the closely related partitiviruses Fusarium poae virus 1 and Penicillium stoloniferum virus F. These findings provide new insights into partitivirus biological activities and clues about molecular interactions between partitiviruses and their hosts.ImportanceMembers of the Partitiviridae are believed to occur commonly in their phytopathogenic fungal and plant hosts. However, most partitiviruses examined so far appear to be associated with latent infections. Here we report a partitivirus, SsPV1/WF-1, that was isolated from a hypovirulent strain of Sclerotinia sclerotiorum and describe its biological and molecular features. We have demonstrated that SsPV1 confers hypovirulence. Furthermore, SsPV1 can infect and cause hypovirulence in Botrytis cinerea. Our study also suggests that SsPV1 has a vigorous ability to proliferate and spread via hyphal contact. SsPV1 can overcome vegetative incompatibility barriers and can be transmitted horizontally among different vegetative compatibility groups of S. sclerotiorum, even interspecifically. Cryo-EM reconstruction of SsPV1 shows that it has a distinct structure with similarity to closely related partitiviruses. Our studies exploit a novel system, SsPV1 and its hosts, which can provide the means to explore the mechanisms by which partitiviruses interact with their hosts.
- Published
- 2014
135. Three-dimensional reconstructions of the bacteriophage CUS-3 virion reveal a conserved coat protein I-domain but a distinct tailspike receptor-binding domain.
- Author
-
Parent, Kristin N, Tang, Jinghua, Cardone, Giovanni, Gilcrease, Eddie B, Janssen, Mandy E, Olson, Norman H, Casjens, Sherwood R, and Baker, Timothy S
- Subjects
Bacteriophages ,Virion ,Capsid Proteins ,Cryoelectron Microscopy ,Phylogeny ,Amino Acid Sequence ,Conserved Sequence ,Protein Structure ,Tertiary ,Protein Binding ,Models ,Molecular ,Molecular Sequence Data ,Bacteriophage ,CUS-3 ,Cryo-electron microscopy ,Virion structure ,Biological Sciences ,Agricultural and Veterinary Sciences ,Medical and Health Sciences ,Virology - Abstract
CUS-3 is a short-tailed, dsDNA bacteriophage that infects serotype K1 Escherichia coli. We report icosahedrally averaged and asymmetric, three-dimensional, cryo-electron microscopic reconstructions of the CUS-3 virion. Its coat protein structure adopts the "HK97-fold" shared by other tailed phages and is quite similar to that in phages P22 and Sf6 despite only weak amino acid sequence similarity. In addition, these coat proteins share a unique extra external domain ("I-domain"), suggesting that the group of P22-like phages has evolved over a very long time period without acquiring a new coat protein gene from another phage group. On the other hand, the morphology of the CUS-3 tailspike differs significantly from that of P22 or Sf6, but is similar to the tailspike of phage K1F, a member of the extremely distantly related T7 group of phages. We conclude that CUS-3 obtained its tailspike gene from a distantly related phage quite recently.
- Published
- 2014
136. OmpA and OmpC are critical host factors for bacteriophage Sf6 entry in Shigella
- Author
-
Parent, Kristin N, Erb, Marcella L, Cardone, Giovanni, Nguyen, Katrina, Gilcrease, Eddie B, Porcek, Natalia B, Pogliano, Joe, Baker, Timothy S, and Casjens, Sherwood R
- Subjects
Microbiology ,Biochemistry and Cell Biology ,Biological Sciences ,Digestive Diseases ,Infectious Diseases ,Biodefense ,Emerging Infectious Diseases ,2.1 Biological and endogenous factors ,Aetiology ,Infection ,Bacterial Outer Membrane Proteins ,Bacteriophages ,Electron Microscope Tomography ,Genome ,Viral ,Lipopolysaccharides ,Microscopy ,Fluorescence ,Mutation ,Shigella flexneri ,Virion ,Agricultural and Veterinary Sciences ,Medical and Health Sciences ,Biological sciences - Abstract
Despite being essential for successful infection, the molecular cues involved in host recognition and genome transfer of viruses are not completely understood. Bacterial outer membrane proteins A and C co-purify in lipid vesicles with bacteriophage Sf6, implicating both outer membrane proteins as potential host receptors. We determined that outer membrane proteins A and C mediate Sf6 infection by dramatically increasing its rate and efficiency. We performed a combination of in vivo studies with three omp null mutants of Shigella flexneri, including classic phage plaque assays and time-lapse fluorescence microscopy to monitor genome ejection at the single virion level. Cryo-electron tomography of phage 'infecting' outer membrane vesicles shows the tail needle contacting and indenting the outer membrane. Lastly, in vitro ejection studies reveal that lipopolysaccharide and outer membrane proteins are both required for Sf6 genome release. We conclude that Sf6 phage entry utilizes either outer membrane proteins A or C, with outer membrane protein A being the preferred receptor.
- Published
- 2014
137. Dynamic and geometric analyses of Nudaurelia capensis ω virus maturation reveal the energy landscape of particle transitions
- Author
-
Tang, Jinghua, Kearney, Bradley M, Wang, Qiu, Doerschuk, Peter C, Baker, Timothy S, and Johnson, John E
- Subjects
Animals ,Capsid ,Insect Viruses ,Insecta ,Protein Structure ,Quaternary ,RNA Viruses ,Virion ,Virus Latency ,autocatalysis ,cryoEM ,virus maturation ,virus capsid quasi-equivalence ,maximum likelihood estimation ,variance map ,NV ,virus particle dynamics ,NωV ,Chemical Sciences ,Biophysics - Abstract
Quasi-equivalent viruses that infect animals and bacteria require a maturation process in which particles transition from initially assembled procapsids to infectious virions. Nudaurelia capensis ω virus (NωV) is a T = 4, eukaryotic, single-stranded ribonucleic acid virus that has proved to be an excellent model system for studying the mechanisms of viral maturation. Structures of NωV procapsids (diameter = 480 Å), a maturation intermediate (410 Å), and the mature virion (410 Å) were determined by electron cryo-microscopy and three-dimensional image reconstruction (cryoEM). The cryoEM density for each particle type was analyzed with a recently developed maximum likelihood variance (MLV) method for characterizing microstates occupied in the ensemble of particles used for the reconstructions. The procapsid and the mature capsid had overall low variance (i.e., uniform particle populations) while the maturation intermediate (that had not undergone post-assembly autocatalytic cleavage) had roughly two to four times the variance of the first two particles. Without maturation cleavage, the particles assume a variety of microstates, as the frustrated subunits cannot reach a minimum energy configuration. Geometric analyses of subunit coordinates provided a quantitative description of the particle reorganization during maturation. Superposition of the four quasi-equivalent subunits in the procapsid had an average root mean square deviation (RMSD) of 3 Å while the mature particle had an RMSD of 11 Å, showing that the subunits differentiate from near equivalent environments in the procapsid to strikingly non-equivalent environments during maturation. Autocatalytic cleavage is clearly required for the reorganized mature particle to reach the minimum energy state required for stability and infectivity.
- Published
- 2014
138. Single particle analysis integrated with microscopy: A high-throughput approach for reconstructing icosahedral particles
- Author
-
Yan, Xiaodong, Cardone, Giovanni, Zhang, Xing, Zhou, Z Hong, and Baker, Timothy S
- Subjects
Biochemistry and Cell Biology ,Biological Sciences ,Networking and Information Technology R&D (NITRD) ,Bioengineering ,Computer Simulation ,Cryoelectron Microscopy ,Imaging ,Three-Dimensional ,Models ,Molecular ,Software ,Virion ,Cryo-electron microscopy ,Image processing ,Single particle analysis ,Icosahedral particles ,3D reconstruction ,Automation ,Zoology ,Biophysics ,Biochemistry and cell biology - Abstract
In cryo-electron microscopy and single particle analysis, data acquisition and image processing are generally carried out in sequential steps and computation of a three-dimensional reconstruction only begins once all the micrographs have been acquired. We are developing an integrated system for processing images of icosahedral particles during microscopy to provide reconstructed density maps in real-time at the highest possible resolution. The system is designed as a combination of pipelines to run in parallel on a computer cluster and analyzes micrographs as they are acquired, handling automatically all the processing steps from defocus estimation and particle picking to origin/orientation determination. An ab initio model is determined independently from the first micrographs collected, and new models are generated as more particles become available. As a proof of concept, we simulated data acquisition sessions using three sets of micrographs of good to excellent quality that were previously recorded from different icosahedral viruses. Results show that the processing of single micrographs can keep pace with an acquisition rate of about two images per minute. The reconstructed density map improves steadily during the image acquisition phase and its quality at the end of data collection is only moderately inferior to that obtained by expert users who processed semi-automatically all the micrographs after the acquisition. The current prototype demonstrates the advantages of integrating three-dimensional image processing with microscopy, which include an ability to monitor acquisition in terms of the final structure and to predict how much data and microscope resources are needed to achieve a desired resolution.
- Published
- 2014
139. Understanding different dominance patterns in western Amazonian forests.
- Author
-
Matas‐Granados, Laura, Draper, Frederick C., Cayuela, Luis, de Aledo, Julia G., Arellano, Gabriel, Saadi, Celina Ben, Baker, Timothy R., Phillips, Oliver L., Honorio Coronado, Eurídice N., Ruokolainen, Kalle, García‐Villacorta, Roosevelt, Roucoux, Katherine H., Guèze, Maximilien, Sandoval, Elvis Valderrama, Fine, Paul V. A., Amasifuen Guerra, Carlos A., Gomez, Ricardo Zarate, Stevenson Diaz, Pablo R., Monteagudo‐Mendoza, Abel, and Martinez, Rodolfo Vasquez
- Subjects
FOREST surveys ,SPECIES diversity ,COMPETITION (Biology) - Abstract
Dominance of neotropical tree communities by a few species is widely documented, but dominant trees show a variety of distributional patterns still poorly understood. Here, we used 503 forest inventory plots (93,719 individuals ≥2.5 cm diameter, 2609 species) to explore the relationships between local abundance, regional frequency and spatial aggregation of dominant species in four main habitat types in western Amazonia. Although the abundance‐occupancy relationship is positive for the full dataset, we found that among dominant Amazonian tree species, there is a strong negative relationship between local abundance and regional frequency and/or spatial aggregation across habitat types. Our findings suggest an ecological trade‐off whereby dominant species can be locally abundant (local dominants) or regionally widespread (widespread dominants), but rarely both (oligarchs). Given the importance of dominant species as drivers of diversity and ecosystem functioning, unravelling different dominance patterns is a research priority to direct conservation efforts in Amazonian forests. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
140. La impresionante diversidad y estructura del bosque tropical a través de una gradiente altitudinal en la selva central del Perú
- Author
-
Monteagudo, Abel, primary, Villalba, María, additional, Pallqui, Nadir, additional, Phillips, Oliver, additional, Baker, Timothy, additional, Lopez-Gonzalez, Gabriela, additional, Pickavance, Georgia, additional, Chávez, Wilfredo, additional, Vásquez, Rodolfo, additional, Rojas, Rocío, additional, Valenzuela, Luis, additional, Chama, Víctor, additional, Catchpole, Damien, additional, Huamantupa, Isau, additional, Soto, Yahn, additional, Ramos, Álvaro, additional, Ramírez, Carolina, additional, Pedraza, Miguel, additional, Huari, Gisela, additional, Banda, Karina, additional, Honorio, Eurídice, additional, Farfán-Rios, William, additional, Dueñas, Hugo, additional, Monteagudo, Rolando, additional, Calatayud, Gloria, additional, Garate ,, Jorge, additional, and Marca-Zevallos, Manuel J., additional
- Published
- 2023
- Full Text
- View/download PDF
141. Effects of an In Vivo Vaping Challenge on In Vitro Interleukin‐6 Biosynthesis Pathways in Arterial Endothelial Cells Derived From Human Embryonic Stem Cells
- Author
-
Tattersall, Matthew C., primary, Esnault, Stephane, additional, Stewart, Ron, additional, Vereide, David T., additional, Swanson, Scott, additional, Zhang, Jue, additional, Steill, John, additional, Jarjour, Nizar, additional, Hansen, Kristin M., additional, Korcarz, Claudia E., additional, Baker, Timothy B., additional, and Stein, James H., additional
- Published
- 2023
- Full Text
- View/download PDF
142. Pervasive gaps in Amazonian ecological research
- Author
-
Carvalho, Raquel L., primary, Resende, Angelica F., additional, Barlow, Jos, additional, França, Filipe M., additional, Moura, Mario R., additional, Maciel, Rafaella, additional, Alves-Martins, Fernanda, additional, Shutt, Jack, additional, Nunes, Cassio A., additional, Elias, Fernando, additional, Silveira, Juliana M., additional, Stegmann, Lis, additional, Baccaro, Fabricio B., additional, Juen, Leandro, additional, Schietti, Juliana, additional, Aragão, Luiz, additional, Berenguer, Erika, additional, Castello, Leandro, additional, Costa, Flavia R.C., additional, Guedes, Matheus L., additional, Leal, Cecilia G., additional, Lees, Alexander C., additional, Isaac, Victoria, additional, Nascimento, Rodrigo O., additional, Phillips, Oliver L., additional, Schmidt, Fernando Augusto, additional, ter Steege, Hans, additional, Vaz-de-Mello, Fernando, additional, Venticinque, Eduardo M., additional, Vieira, Ima Célia Guimarães, additional, Zuanon, Jansen, additional, Ferreira, Joice, additional, Carvalho, Raquel L., additional, França, Filipe, additional, Geber Filho, Adem Nagibe dos Santos, additional, Ruschel, Ademir, additional, Calor, Adolfo Ricardo, additional, de Lima Alves, Adriana, additional, Muelbert, Adriane Esquivel, additional, Quaresma, Adriano, additional, Vicentini, Alberto, additional, Piedade, Alexandra Rocha da, additional, Oliveira, Alexandre Adalardo de, additional, Aleixo, Alexandre, additional, Casadei-Ferreira, Alexandre, additional, Gontijo, Alexandre, additional, Hercos, Alexandre, additional, Andriolo, Aline, additional, Lopes, Aline, additional, Pontes-Lopes, Aline, additional, Santos, Allan Paulo Moreira dos, additional, Oliveira, Amanda Batista da Silva de, additional, Mortati, Amanda Frederico, additional, Salcedo, Ana Karina Moreyra, additional, Albernaz, Ana Luisa, additional, Fares, Ana Luisa, additional, Andrade, Ana Luiza, additional, Oliveira Pes, Ana Maria, additional, Faria, Ana Paula Justino, additional, Batista, Anderson Pedro Bernadina, additional, Puker, Anderson, additional, Bueno, Anderson S., additional, Junqueira, André Braga, additional, Holanda de Andrade, André Luiz Ramos, additional, Ghidini, André Ricardo, additional, Galuch, André V., additional, Menezes, Andressa Silvana Oliveira de, additional, Manzatto, Angelo Gilberto, additional, Correa, Anne Sthephane A.S., additional, Queiroz, Antonio C.M., additional, Zanzini, Antonio Carlos da Silva, additional, Olivo Neto, Antonio Miguel, additional, Melo, Antonio Willian Flores de, additional, Guimaraes, Aretha Franklin, additional, Castro, Arlison Bezerra, additional, Borges, Augusto, additional, Ferreira, Aurélia Bentes, additional, Marimon, Beatriz S., additional, Marimon-Junior, Ben Hur, additional, Flores, Bernardo M., additional, de Resende, Bethânia Oliveira, additional, Albuquerque, Bianca Weiss, additional, Villa, Boris, additional, Davis, Bradley, additional, Nelson, Bruce, additional, Williamson, Bruce, additional, Melo, Bruna Santos Bitencourt de, additional, Cintra, Bruno B.L., additional, Santos, Bruno Borges, additional, Prudente, Bruno da Silveira, additional, Luize, Bruno Garcia, additional, Godoy, Bruno Spacek, additional, Rutt, Cameron L., additional, Duarte Ritter, Camila, additional, Silva, Camila V.J., additional, Ribas, Carla Rodrigues, additional, Peres, Carlos A., additional, Azevêdo, Carlos Augusto Silva de, additional, Freitas, Carlos, additional, Cordeiro, Carlos Leandro, additional, Brocardo, Carlos Rodrigo, additional, Castilho, Carolina, additional, Levis, Carolina, additional, Doria, Carolina Rodrigues da Costa, additional, Arantes, Caroline C., additional, Santos, Cássia Anicá dos, additional, Jakovac, Catarina C., additional, Silva, Celice Alexandre, additional, Benetti, Cesar João, additional, Lasmar, Chaim, additional, Marsh, Charles J., additional, Andretti, Christian Borges, additional, Oliveira, Cinthia Pereira de, additional, Cornelius, Cintia, additional, Alves da Rosa, Clarissa, additional, Baider, Cláudia, additional, Gualberto, Cláudia G., additional, Deus, Claudia Pereira de, additional, Monteiro Jr., Cláudio da Silva, additional, Santos Neto, Cláudio Rabelo dos, additional, Lobato, Cleonice Maria Cardoso, additional, Santos, Cleverson Rannieri Meira dos, additional, Penagos, Cristian Camilo Mendoza, additional, Costa, Daniel da Silva, additional, Vieira, Daniel Luis Mascia, additional, Aguiar, Daniel Praia Portela de, additional, Veras, Daniel Silas, additional, Pauletto, Daniela, additional, Braga, Danielle de Lima, additional, Storck-Tonon, Danielle, additional, Almeida, Daniely da Frota, additional, Douglas, Danyhelton, additional, Amaral, Dário Dantas do, additional, Gris, Darlene, additional, Luther, David, additional, Edwards, David P., additional, Guimarães, David Pedroza, additional, Santos, Deane Cabral dos, additional, Campana, Débora Rodrigues de Souza, additional, Nogueira, Denis Silva, additional, Silva, Dennis Rodrigues da, additional, Dutra, Dhâmyla Bruna de Souza, additional, Rosa, Dian Carlos Pinheiro, additional, Silva, Diego Armando Silva da, additional, Pedroza, Diego, additional, Anjos, Diego V., additional, Melo Lima, Diego Viana, additional, Silvério, Divino V., additional, Rodrigues, Domingos de Jesus, additional, Bastos, Douglas, additional, Daly, Douglas, additional, Barbosa, Edelcilio Marques, additional, Arenas, Edith Rosario Clemente, additional, Oliveira, Edmar Almeida de, additional, Santos, Ednaira Alencar dos, additional, Santana, Edrielly Carolinne Carvalho de, additional, Guilherme, Edson, additional, Vidal, Edson, additional, Campos-Filho, Eduardo Malta, additional, van den Berg, Eduardo, additional, Morato, Elder Ferreira, additional, da Silva, Elidiomar R., additional, Marques, Elineide E., additional, Pringle, Elizabeth G., additional, Nichols, Elizabeth, additional, Andresen, Ellen, additional, Farias, Emanuelle de Sousa, additional, Siqueira, Emely Laiara Silva de, additional, de Albuquerque, Emília Zoppas, additional, Görgens, Eric Bastos, additional, Cunha, Erlane José Rodrigues da, additional, Householder, Ethan, additional, Novo, Evlyn Márcia Moraes de Leão, additional, Oliveira, Fabiana Ferreira de, additional, Roque, Fabio de Oliveira, additional, Coletti, Fabrício, additional, Reis, Fagno, additional, Moreira, Felipe F.F., additional, Todeschini, Felipe, additional, Carvalho, Fernanda Antunes, additional, Coelho de Souza, Fernanda, additional, Silva, Fernando Augusto Barbosa, additional, Carvalho, Fernando Geraldo, additional, Cabeceira, Fernando Gonçalves, additional, d’Horta, Fernando Mendonça, additional, Mendonça, Fernando P., additional, Florêncio, Fernando Prado, additional, Carvalho, Fernando Rogério de, additional, Arruda, Filipe Viegas de, additional, Nonato, Flávia Alessandra da Silva, additional, Santana, Flávia Delgado, additional, Durgante, Flavia, additional, Souza, Flávia Kelly Siqueira de, additional, Obermuller, Flávio Amorim, additional, Castro, Flávio Siqueira de, additional, Wittmann, Florian, additional, Sales, Francisco Matheus da Silva, additional, Neto, Francisco Valente-, additional, Salles, Frederico Falcão, additional, Borba, Gabriel Costa, additional, Damasco, Gabriel, additional, Barros, Gabriel Gazzana, additional, Brejão, Gabriel Lourenço, additional, Jardim, Gabriela Abrantes, additional, Prance, Ghillean T., additional, Lima, Gisiane Rodrigues, additional, Desidério, Gleison Robson, additional, Melo, Gracilene da Costa de, additional, Carmo, Guilherme Henrique Pompiano do, additional, Cabral, Guilherme Sampaio, additional, Rousseau, Guillaume Xavier, additional, da Silva, Gustavo Cardoso, additional, Schwartz, Gustavo, additional, Griffiths, Hannah, additional, Queiroz, Helder Lima de, additional, Espírito-Santo, Helder M.V., additional, Cabette, Helena Soares Ramos, additional, Nascimento, Henrique Eduardo Mendonça, additional, Vasconcelos, Heraldo L., additional, Medeiros, Herison, additional, Aguiar, Hilton Jeferson Alves Cardoso de, additional, Leão, Híngara, additional, Wilker, Icaro, additional, Gonçalves, Inês Correa, additional, de Sousa Gorayeb, Inocêncio, additional, Miranda, Ires Paula de Andrade, additional, Brown, Irving Foster, additional, Santos, Isis Caroline Siqueira, additional, Fernandes, Itanna Oliveira, additional, Fernandes, Izaias, additional, Delabie, Jacques Hubert Charles, additional, de Abreu, Jadson Coelho, additional, Gama Neto, Jaime de Liege, additional, Costa, Janaina Barbosa Pedrosa, additional, Noronha, Janaína Costa, additional, de Brito, Janaina Gomes, additional, Wolfe, Jared, additional, Santos, Jean Carlos, additional, Ferreira-Ferreira, Jefferson, additional, e Gomes, Jerrian Oliveira, additional, Lasky, Jesse R., additional, de Faria Falcão, Jéssica Caroline, additional, Costa, Jessica Gomes, additional, Cravo, Jessica Soares, additional, Guerrero, Jesús Enrique Burgos, additional, Muñoz Gutiérrez, Jhonatan Andrés, additional, Carreiras, João, additional, Lanna, João, additional, Silva Brito, Joás, additional, Schöngart, Jochen, additional, Mendes Aguiar, Jonas José, additional, Lima, Jônatas, additional, Barroso, Jorcely G., additional, Noriega, Jorge Ari, additional, Pereira, Jorge Luiz da Silva, additional, Nessimian, Jorge Luiz, additional, Souza, Jorge Luiz Pereira de, additional, de Toledo, José Julio, additional, Magalhães, José Leonardo Lima, additional, Camargo, José Luís, additional, Oliveira, José Max B., additional, Ribeiro, José Moacir Ferreira, additional, Silva, José Orlando de Almeida, additional, da Silva Guimarães, José Renan, additional, Hawes, Joseph E., additional, Andrade-Silva, Joudellys, additional, Revilla, Juan David Cardenas, additional, da Silva, Júlia Santana, additional, da Silva Menger, Juliana, additional, Rechetelo, Juliana, additional, Stropp, Juliana, additional, Barbosa, Julianna Freires, additional, do Vale, Julio Daniel, additional, Louzada, Julio, additional, Cerqueira Silva, Július César, additional, da Silva, Karina Dias, additional, Melgaço, Karina, additional, Carvalho, Karine Santana, additional, Yamamoto, Kedma Cristine, additional, Mendes, Keila Rêgo, additional, Vulinec, Kevina, additional, Maia, Laís Ferreira, additional, Cavalheiro, Larissa, additional, Vedovato, Laura Barbosa, additional, Demarchi, Layon Oreste, additional, Giacomin, Leandro, additional, Dumas, Leandro Lourenço, additional, Maracahipes, Leandro, additional, Brasil, Leandro Schlemmer, additional, Ferreira, Leandro Valle, additional, Calvão, Lenize Batista, additional, Maracahipes-Santos, Leonardo, additional, Reis, Leonardo Pequeno, additional, da Silva, Letícia Fernandes, additional, de Oliveira Melo, Lia, additional, Carvalho, Lidiany Camila da Silva, additional, Casatti, Lílian, additional, Amado, Lílian Lund, additional, de Matos, Liliane Stedile, additional, Vieira, Lisandro, additional, Prado, Livia Pires do, additional, Alencar, Luana, additional, Fontenele, Luane, additional, Mazzei, Lucas, additional, Navarro Paolucci, Lucas, additional, Zanzini, Lucas Pereira, additional, Carvalho, Lucélia Nobre, additional, Crema, Luciana Carvalho, additional, Brulinger, Luciane Ferreira Barbosa, additional, Montag, Luciano Fogaça de Assis, additional, Naka, Luciano Nicolas, additional, Azara, Ludson, additional, Silveira, Luis Fábio, additional, Nunes, Luis Gabriel de Oliveira, additional, Rosalino, Luís Miguel do Carmo, additional, Mestre, Luiz A.M., additional, Bonates, Luiz Carlos de Matos, additional, Coelho, Luiz de Souza, additional, Borges, Luiz Henrique Medeiros, additional, Lourenço, Luzia da Silva, additional, Freitas, Madson Antonio Benjamin, additional, Brito, Maiara Tábatha da Silva, additional, Pombo, Maihyra Marina, additional, da Rocha, Maíra, additional, Cardoso, Maira Rodrigues, additional, Guedes, Marcelino Carneiro, additional, Raseira, Marcelo Bassols, additional, Medeiros, Marcelo Brilhante de, additional, Carim, Marcelo de Jesus Veiga, additional, Simon, Marcelo Fragomeni, additional, Pansonato, Marcelo Petratti, additional, dos Anjos, Marcelo Rodrigues, additional, Nascimento, Marcelo Trindade, additional, Souza, Márcia Regina de, additional, Monteiro, Marcília Gabriella Tavares, additional, da Silva, Márcio Joaquim, additional, Uehara-Prado, Marcio, additional, Oliveira, Marco Antonio de, additional, Callisto, Marcos, additional, Vital, Marcos José Salgado, additional, o Santos, Marcos Pérsi Dantas, additional, Silveira, Marcos, additional, Oliveira, Marcus Vinicio Neves D., additional, Pérez-Mayorga, María Angélica, additional, Carniello, Maria Antonia, additional, Lopes, Maria Aparecida, additional, Silveira, Maria Aurea Pinheiro de Almeida, additional, Esposito, Maria Cristina, additional, Maldaner, Maria Eduarda, additional, Passos, Maria Inês S., additional, Anacléto, Maria José Pinheiro, additional, Costa, Maria Katiane Sousa, additional, Martins, Maria Pires, additional, Piedade, Maria Teresa Fernandez, additional, Irume, Mariana Victória, additional, Costa, Marília Maria Silva da, additional, Maximiano, Marina Franco de Almeida, additional, Freitas, Marina Guimarães, additional, Cochrane, Mark A., additional, Gastauer, Markus, additional, Almeida, Marllus Rafael Negreiros, additional, Souza, Mateus Fernando de, additional, Catarino, Michel, additional, Costa Batista, Michela, additional, Massam, Mike R., additional, Martins, Mila Ferraz de Oliveira, additional, Holmgren, Milena, additional, Almeida, Morgana, additional, Dias, Murilo S., additional, Espírito Santo, Nádia Barbosa, additional, Benone, Naraiana Loureiro, additional, Ivanauskas, Natalia Macedo, additional, Medeiros, Natália, additional, Targhetta, Natalia, additional, Félix, Nathalia Silva, additional, Ferreira, Nelson, additional, Hamada, Neusa, additional, Campos, Nubia, additional, Giehl, Nubia França da Silva, additional, Metcalf, Oliver Charles, additional, Silva, Otávio Guilherme Morais da, additional, Cerqueira, Pablo Vieira, additional, Moser, Pamela, additional, Miranda, Patrícia Nakayama, additional, Peruquetti, Patricia Santos Ferreira, additional, Alverga, Paula Palhares de Polari, additional, Prist, Paula, additional, Souto, Paula, additional, Brando, Paulo, additional, Pompeu, Paulo dos Santos, additional, Barni, Paulo Eduardo, additional, Graça, Paulo Mauricio de Alencastro, additional, Morandi, Paulo S., additional, Cruz, Paulo Vilela, additional, i Silva, Pedro Giovân da, additional, Bispo, Pitágoras C., additional, Camargo, Plínio Barbosa de, additional, Sarmento, Priscila S. de M., additional, Souza, Priscila, additional, Andrade, Rafael Barreto de, additional, Braga, Rafael Benzi, additional, Boldrini, Rafael, additional, Bastos, Rafael Costa, additional, Assis, Rafael Leandro de, additional, Salomão, Rafael P., additional, Leitão, Rafael Pereira, additional, Mendes, Raimundo N.G., additional, Carpanedo, Rainiellen de Sá, additional, Melinski, Ramiro Dário, additional, Ligeiro, Raphael, additional, e Pérez, Raúl Enriqu Pirela, additional, Barbosa, Reinaldo Imbrozio, additional, Cajaiba, Reinaldo Lucas, additional, Silvano, Renato Azevedo Matias, additional, Salomão, Renato Portela, additional, Hilário, Renato Richard, additional, Martins, Renato Tavares, additional, Perdiz, Ricardo de Oliveira, additional, Vicente, Ricardo Eduardo, additional, Silva, Ricardo José da, additional, Koroiva, Ricardo, additional, Solar, Ricardo, additional, Silva, Richarlly da Costa, additional, s de Lima, Robson Borge, additional, Silva, Robson dos Santos Alves da, additional, Mariano, Rodolfo, additional, Ribeiro, Rodrigo Arison Barbosa, additional, Fadini, Rodrigo Ferreira, additional, Oliveira, Rodrigo Leonardo Costa de, additional, Feitosa, Rodrigo Machado, additional, Matavelli, Rodrigo, additional, Mormul, Roger Paulo, additional, da Silva, Rogério Rosa, additional, Zanetti, Ronald, additional, Barthem, Ronaldo, additional, Almeida, Rony Peterson Santos, additional, Ribeiro, Sabina Cerruto, additional, r Costa Neto, Salustiano Vila da, additional, Nienow, Samuel, additional, Oliveira, Sérgio Augusto Vidal de, additional, Borges, Sérgio Henrique, additional, Milheiras, Sérgio, additional, Ribeiro, Sérvio Pontes, additional, Couceiro, Sheyla Regina Marques, additional, Sousa, Sidney Araújo de, additional, Rodrigues, Silvia Barbosa, additional, Dutra, Silvia Leitão, additional, Mahood, Simon, additional, Vieira, Simone Aparecida, additional, Arrolho, Solange, additional, Silva, Sonaira Souza da, additional, Triana, Stefania Pinzón, additional, Laurance, Susan, additional, Kunz, Sustanis Horn, additional, Alvarado, Swanni T., additional, Rodrigues, Taís Helena Araujo, additional, Santos, Talitha Ferreira dos, additional, Machado, Tatiana Lemos da Silva, additional, Feldpausch, Ted R., additional, Sousa, Thaiane, additional, Michelan, Thaisa Sala, additional, Emilio, Thaise, additional, Brito, Thaline de Freitas, additional, André, Thiago, additional, Barbosa, Thiago Augusto Pedroso, additional, Miguel, Thiago Barros, additional, Izzo, Thiago Junqueira, additional, Laranjeiras, Thiago Orsi, additional, Mendes, Thiago Pereira, additional, Silva, Thiago Sanna Freire, additional, Krolow, Tiago Kütter, additional, Begot, Tiago Octavio, additional, Baker, Timothy R., additional, Domingues, Tomas F., additional, Giarrizzo, Tommaso, additional, Bentos, Tony Vizcarra, additional, Haugaasen, Torbjørn, additional, Peixoto, Ualerson, additional, Pozzobom, Ully Mattilde, additional, Korasaki, Vanesca, additional, Ribeiro, Vanessa Soares, additional, Scudeller, Veridiana Vizoni, additional, Oliveira, Victor Hugo Fonseca, additional, Landeiro, Victor Lemes, additional, Santos Ferreira, Victor Rennan, additional, Silva, Victória de Nazaré Gama, additional, Gomes, Vitor Hugo Freitas, additional, Oliveira, Vívian Campos de, additional, Firmino, Viviane, additional, Santiago, Wagner Tadeu Vieira, additional, Beiroz, Wallace, additional, Almeida, Wanessa Rejane de, additional, Oliveira, Washington Luis de, additional, Silva, Wegliane Campelo da, additional, Castro, Wendeson, additional, Dáttilo, Wesley, additional, Cruz, Wesley Jonatar Alves da, additional, Silva, Wheriton Fernando Moreira da, additional, Magnusson, William E., additional, Laurance, William, additional, Milliken, William, additional, Paula, William Sousa de, additional, Malhi, Yadvinder, additional, Shimabukuro, Yosio Edemir, additional, Lima, Ysadhora Gomes de, additional, Shimano, Yulie, additional, and Feitosa, Yuri, additional
- Published
- 2023
- Full Text
- View/download PDF
143. Using tree species inventories to map biomes and assess their climatic overlaps in lowland tropical South America
- Author
-
de Miranda, Pedro Luiz Silva, Oliveira-Filho, Ary T., Pennington, R. Toby, Neves, Danilo M., Baker, Timothy R., and Dexter, Kyle G.
- Published
- 2018
144. Leveraging Genomic Data in Smoking Cessation Trials in the Era of Precision Medicine : Why and How
- Author
-
Genetics and Treatment Workgroup of the Society for Research on Nicotine and Tobacco (SRNT), Chen, Li-Shiun, Zawertailo, Laurie, Piasecki, Thomas M., Kaprio, Jaakko, Foreman, Marilyn, Elliott, Hannah R., David, Sean P., Bergen, Andrew W., Baurley, James W., Tyndale, Rachel F., Baker, Timothy B., Bierut, Laura J., and Saccone, Nancy L.
- Published
- 2018
145. Monastic Preaching and the Sermon in Medieval Latin Christendom to the Twelfth Century
- Author
-
Baker, Timothy M., primary and Kienzle, Beverly, additional
- Published
- 2020
- Full Text
- View/download PDF
146. Companion Animals in Contemporary Scottish Women’s Gothic
- Author
-
Baker, Timothy C., primary
- Published
- 2019
- Full Text
- View/download PDF
147. MIF and Pulmonary Disease
- Author
-
Baker, Timothy, Lee, Patty J., Sauler, Maor, Parnham, Michael J., Series editor, Schmidtko, Achim, Series editor, Bucala, Richard, editor, and Bernhagen, Jürgen, editor
- Published
- 2017
- Full Text
- View/download PDF
148. Is the Fagerström test for nicotine dependence invariant across secular trends in smoking? A question for cross-birth cohort analysis of nicotine dependence
- Author
-
Glasheen, Cristie, Johnson, Eric O., Saccone, Nancy L., Lutz, Sharon M., Baker, Timothy B., McNeil, Daniel W., Marazita, Mary L., Hokanson, John E., Bierut, Laura J., and Hancock, Dana B.
- Published
- 2018
- Full Text
- View/download PDF
149. Genetic correlation between smoking behaviors and schizophrenia
- Author
-
Hartz, Sarah M., Horton, Amy C., Hancock, Dana B., Baker, Timothy B., Caporaso, Neil E., Chen, Li-Shiun, Hokanson, John E., Lutz, Sharon M., Marazita, Mary L., McNeil, Daniel W., Pato, Carlos N., Pato, Michele T., Johnson, Eric O., and Bierut, Laura J.
- Published
- 2018
- Full Text
- View/download PDF
150. Three-dimensional reconstruction of icosahedral particles from single micrographs in real time at the microscope
- Author
-
Cardone, Giovanni, Yan, Xiaodong, Sinkovits, Robert S, Tang, Jinghua, and Baker, Timothy S
- Subjects
Biochemistry and Cell Biology ,Biological Sciences ,Biotechnology ,Bioengineering ,Bacteriophage P22 ,Capsid ,Cryoelectron Microscopy ,Imaging ,Three-Dimensional ,Models ,Molecular ,Protein Structure ,Quaternary ,Reproducibility of Results ,Software ,Cryo-electron microscopy ,Image processing ,Single particle analysis ,Icosahedral particles ,3D reconstruction ,Automation ,Zoology ,Biophysics ,Biochemistry and cell biology - Abstract
Single particle analysis is a valuable tool in cryo-electron microscopy for determining the structure of biological complexes. However, the conformational state and the preparation of the sample are factors that play a critical role in the ultimate attainable resolution. In some cases extensive analysis at the microscope of a sample under different conditions is required to derive the optimal acquisition conditions. Currently this analysis is limited to raw micrographs, thus conveying only limited information on the structure of the complex. We are developing a computing system that generates a three-dimensional reconstruction from a single micrograph acquired under cryogenic and low dose conditions, and containing particles with icosahedral symmetry. The system provides the microscopist with immediate structural information from a sample while it is in the microscope and during the preliminary acquisition stage. The system is designed to run without user intervention on a multi-processor computing resource and integrates all the processing steps required for the analysis. Tests performed on experimental data sets show that the probability of obtaining a reliable reconstruction from one micrograph is primarily determined by the quality of the sample, with success rates close to 100% when sample conditions are optimal, and decreasing to about 60% when conditions are sub-optimal. The time required to generate a reconstruction depends significantly on the diameter of the particles, and in most instances takes about 1min. The proposed approach can provide valuable three-dimensional information, albeit at low resolution, on conformational states, epitope binding, and stoichiometry of icosahedral multi-protein complexes.
- Published
- 2013
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.