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Your search keyword '"Beta galactosidase"' showing total 101 results
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101 results on '"Beta galactosidase"'

101. Immobilized-enzyme activity under isothermal and non-isothermal conditions: Studies on β-galactosidase as a model enzyme

Author

Mita, D. G., Marianna Bianca Emanuela PORTACCIO, Russo, P., Stellato, S., Toscano, G., Bencivenga, U., Canciglia, P., D Acunto, A., Pagliuca, N., Rossi, S., Gaeta, F. S., D., Mita, M., Portaccio, P., Russo, S., Stellato, Toscano, Giuseppe, U., Bencivenga, P., Canciglia, A., D'Acunto, N., Pagliuca, S., Rossi, and F., Gaeta

Subjects
beta galactosidase, Enzyme immobilization, temperature, enzyme activity
Abstract

The behaviour of catalytic reactions was studied when an enzyme is immobilized to a membrane interposed between two solutions of identical substrate concentration, kept at different temperatures. Beta-Galactosidase was studied as a model enzyme. In the presence of a temperature gradient the apparent enzyme activity is higher than in comparable isothermal conditions. The activity increase is proportional to the applied transmembrane temperature difference. Two possible explanations are proposed for this behaviour. Various biotechnological applications are suggested.

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