101. A novel controlled release tetrandrine-loaded PDLLA film: evaluation of drug release and anti-adhesion effects in vitro and in vivo
- Author
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Jian Liu, Hai Yao, Xiaoxing Zhang, Lei Peng, Deng Zhilong, and Cao Zhidong
- Subjects
Simulated body fluid ,Polyesters ,Tetrandrine ,Pharmaceutical Science ,Tissue Adhesions ,02 engineering and technology ,030226 pharmacology & pharmacy ,High-performance liquid chromatography ,Benzylisoquinolines ,03 medical and health sciences ,chemistry.chemical_compound ,Random Allocation ,0302 clinical medicine ,In vivo ,Extracellular ,FBSS ,Animals ,Chromatography, High Pressure Liquid ,Controlled drug release ,Epidural fibrosis ,Chemistry ,Laminectomy ,Adhesion ,021001 nanoscience & nanotechnology ,Controlled release ,In vitro ,Disease Models, Animal ,Delayed-Action Preparations ,Original Article ,Collagen ,Rabbits ,Anti-adhesion film ,0210 nano-technology ,Biomedical engineering - Abstract
To investigate the drug release and anti-adhesion effects of a TET (tetrandrine)-loaded PDLLA (poly-dl-lactide) film. Detection of TET release in vitro was carried out by high-performance liquid chromatography (HPLC) every 2 days following immersion of the tetrandrine-loaded PDLLA film in simulated body fluid until the TET content of the eluate could not be detected. For the in vivo test, TET-loaded PDLLA films were implanted into animal laminectomy models and positive and blank control groups were also set up. Postoperative serum tests, and macroscopic and histological analyses at 1, 4, 8, and 12 weeks, were used to assess the effects of the film. Statistical analyses were performed by one-way ANOVA. The drug release of the tetrandrine-loaded PDLLA film in vitro showed two phases with a second release peak. Ultimately, the duration of continuous delivery was up to 66 days and the cumulative delivery rate was up to 93.18%. Scores for the proliferation of epidural scars or adhesion of the dura mater in the test group were much lower than those for the two control groups. Histological analysis revealed the test group had fewer inflammatory cells and fibroblasts, as well as fewer extracellular collagen fibers, and a lower histology score than those of the two control groups at all time points. Tetrandrine-loaded PDLLA film is a novel controlled drug release and anti-adhesion material in vitro and in vivo.
- Published
- 2019