117 results on '"Carvalho, Humberto M"'
Search Results
102. Does performance in specific motor tests discriminate the selection of young basketball players?
- Author
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Soares, André L. A., Lima, Ahlan B., Possamai, Kauana, Carvalho, Humberto M., and Miguel, Caio G.
- Subjects
ATHLETES ,TASK performance ,CONFERENCES & conventions ,BASKETBALL ,ATHLETIC ability ,MOTOR ability - Abstract
Background: The basketball game is composed of intermittent short and intense actions of sprinting, jumping, and changing directions requiring a good level of physical fitness, strength, and motor coordination. Specific basketball task tests to assess players' motor tasks and performance are commonly used in research and applied contexts to distinguish between-athletes variations. In this study, we considered athletes engaged in structured basketball programs of Santa Catarina, supervised by Federação Catarinense de Basketball (FCB). Aim: We examined whether performance in basketball-specific tasks was sensitive to distinguish between female and male young players selected or not selected to represent the state team that competed at national-level competitions. Furthermore, we adjusted our estimations to the influence of accumulated training experience in basketball and chronological on basketball-specific tasks. Material and methods: This sample comprised 172 young basketball players (female, n = 73; male n = 99) aged 14.5 and 18.5, competing in under-16 and under-18 age groups. We grouped players by sex and competitive level (players from club-level and state-level, i.e., those selected for the Santa Catarina team competing at a national competition). In addition, we considered the following basketballspecific tasks: the countermovement jump without arms (CMJ) as a measure of lower body muscle power, the Line-Drill test (LD) as a measure of agility ability and anaerobic performance, and the 20 m Sprint as a measure of speed. Finally, we modeled the variation on the basketball-specific task between players by competitive level, adjusting for chronological age and accumulated experience by fitting multilevel regression models using a fully Bayesian approach. Results: There was substantial variation in the basketball-specific tasks by sex and older players had better jumping and sprint performance. Also, there was a trend of more experienced players showing a better performance in the LD, although variation was large, suggesting a conservative interpretation. There was no substantial variation between players by competitive level. Conclusion: Although basketball-specific tests are commonly used to interpret players' performance, older players performed better. The specific motor tasks were not sensible to distinguishing athletes by competitive level, independent of sex. The tasks used may be insensitive to distinguish players by competitive level, or the variation in the competitive level in the present sample may be small. On the other hand, the tasks were sensitive to players' differences in chronological age and training experience, suggesting that other factors had more weight on coaches' decisions in selecting players for the state team. [ABSTRACT FROM AUTHOR]
- Published
- 2022
103. Detection of Bacillus anthracisSpore Germination In Vivo by Bioluminescence Imaging
- Author
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Sanz, Patrick, Teel, Louise D., Alem, Farhang, Carvalho, Humberto M., Darnell, Stephen C., and O'Brien, Alison D.
- Abstract
ABSTRACTWe sought to visualize the site of Bacillus anthracisspore germination in vivo. For that purpose, we constructed a reporter plasmid with the luxoperon under control of the spore small acid-soluble protein B (sspB) promoter. In B. subtilis, sspB-driven synthesis of luciferase during sporulation results in incorporation of the enzyme in spores. We observed that B. anthracisSterne transformed with our sspBp::luxplasmid was only luminescent during germination. In contrast, Sterne transformed with a similarly constructed plasmid with luxexpression under control of the protective antigen promoter displayed luminescence only during vegetative growth. We then infected A/J mice intranasally with spores that harbored the germination reporter. Mice were monitored for up to 14 days with the Xenogen In Vivo Imaging System. While luminescence only became evident in live animals at 18 h, dissection after sacrificing infected mice at earlier time points revealed luminescence in lung tissue at 30 min after intranasal infection. Microscopic histochemical and immunofluorescence studies on luminescent lung sections and imprints revealed that macrophages were the first cells in contact with the B. anthracisspores. By 6 h after infection, polymorphonuclear leukocytes with intracellular spores were evident in the alveolar spaces. After 24 h, few free spores were observed in the alveolar spaces; most of the spores detected by immunofluorescence were in the cytoplasm of interstitial macrophages. In contrast, mediastinal lymph nodes remained nonluminescent throughout the infection. We conclude that in this animal system, the primary site of B. anthracisspore germination is the lungs.
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- 2008
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104. Recombinant Exosporium Protein BclA of Bacillus anthracisIs Effective as a Booster for Mice Primed with Suboptimal Amounts of Protective Antigen
- Author
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Brahmbhatt, Trupti N., Darnell, Stephen C., Carvalho, Humberto M., Sanz, Patrick, Kang, Tae J., Bull, Robert L., Rasmussen, Susan B., Cross, Alan S., and O'Brien, Alison D.
- Abstract
ABSTRACTBacillus collagen-like protein of anthracis (BclA) is an immunodominant glycoprotein located on the exosporium of Bacillus anthracis. We hypothesized that antibodies to this spore surface antigen are largely responsible for the augmented immunity to anthrax that has been reported for animals vaccinated with inactivated spores and protective antigen (PA) compared to vaccination with PA alone. To test this theory, we first evaluated the capacity of recombinant, histidine-tagged, nonglycosylated BclA (rBclA) given with adjuvant to protect A/J mice against 10 times the 50% lethal dose of Sterne strain spores introduced subcutaneously. Although the animals elicited anti-rBclA antibodies and showed a slight but statistically significant prolongation in the mean time to death (MTD), none of the mice survived. Similarly, rabbit anti-rBclA immunoglobulin G (IgG) administered intraperitoneally to mice before spore inoculation increased the MTD statistically significantly but afforded protection to only 1 of 10 animals. However, all mice that received suboptimal amounts of recombinant PA and that then received rBclA 2 weeks later survived spore challenge. Additionally, anti-rBclA IgG, compared to anti-PA IgG, promoted a sevenfold-greater uptake of opsonized spores by mouse macrophages and markedly decreased intramacrophage spore germination. Since BclA has some sequence similarity to human collagen, we also tested the extent of binding of anti-rBclA antibodies to human collagen types I, III, and V and found no discernible cross-reactivity. Taken together, these results support the concept of rBclA as being a safe and effective boost for a PA-primed individual against anthrax and further suggest that such rBclA-enhanced protection occurs by the induction of spore-opsonizing and germination-inhibiting antibodies.
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- 2007
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105. Cytotoxic Necrotizing Factor Type 1 Delivered by Outer Membrane Vesicles of Uropathogenic Escherichia coliAttenuates Polymorphonuclear Leukocyte Antimicrobial Activity and Chemotaxis
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Davis, Jon M., Carvalho, Humberto M., Rasmussen, Susan B., and O'Brien, Alison D.
- Abstract
ABSTRACTCytotoxic necrotizing factor type 1 (CNF1), a toxin produced by many strains of uropathogenic Escherichia coli(UPEC), constitutively activates small GTPases of the Rho family by deamidating a single amino acid within these target proteins. Such activated GTPases not only stimulate actin polymerization within affected cells but also, as we previously reported, decrease membrane fluidity on mouse polymorphonuclear leukocytes (PMNs). In that same investigation we found that this diminished membrane movement impedes the clustering of the complement receptor CD11b/CD18 on PMNs and, in turn, decreases PMN phagocytic capacity and microbicidal activity on PMNs in direct contact with CNF1-expressing UPEC as well as on those in proximity to wild-type UPEC. The latter observation suggested to us that CNF1 is released from neighboring bacteria, although at the time of initiation of the study described here, no specific mechanism for export of CNF1 from UPEC had been described. Here we present evidence that CNF1 is released from the CNF1-expressing UPEC strain CP9 (serotype O4/H5/K54) in a complex with outer membrane vesicles (OMVs) and that these CNF1-bearing vesicles transfer biologically active CNF1 to PMNs and attenuate phagocyte function. Furthermore, we show that CNF1-bearing vesicles act in a dose-dependent fashion on PMNs to inhibit their chemotactic response to formyl-Met-Leu-Phe, while purified CNF1 does not. We conclude that OMVs provide a means for delivery of CNF1 from a UPEC strain to PMNs and thus negatively affect the efficacy of the acute inflammatory response to these organisms.
- Published
- 2006
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106. Antibody against the Carboxyl Terminus of Intimin α Reduces Enteropathogenic Escherichia coliAdherence to Tissue Culture Cells and Subsequent Induction of Actin Polymerization
- Author
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Carvalho, Humberto M., Teel, Louise D., Kokai-Kun, John F., and O'Brien, Alison D.
- Abstract
ABSTRACTThe C-terminal third of intimin binds to its translocated receptor (Tir) to promote attaching and effacing lesion formation during infection with enteropathogenic Escherichia coli(EPEC). We observed that the adherence of EPEC strains to HEp-2 cells was reduced and that actin polymerization was blocked by antibody raised against the C-terminal third of intimin α.
- Published
- 2005
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- View/download PDF
107. Why do they engage in such hard programs? The search for excellence in youth basketball
- Author
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Goncalves, Carlos E., Manuel Coelho-e-Silva, Carvalho, Humberto M., and Goncalves, Angela
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lcsh:Sports ,lcsh:GV557-1198.995 ,Youth ,motivation ,expertise ,talent specialization ,lcsh:Sports medicine ,lcsh:RC1200-1245 - Abstract
Excellent performance in sport has a strong positive relationship with the accumulated hours of practice. The specialization years are seen as a decisive moment to lift the skill level, athletic readiness and commitment but the selection and orientation of talent has been strongly dependent of biological and motor variables. The purpose of this study is to describe the achievement and motivation variables that can explain the belonging to an elite competitive level of young basketball players. Eighty-two basketball players under 16 years fulfilled the WOFO Questionnaire (Spence and Helmreich, 1983), and an adapted version of the DPMQ (De Bruin, Rikers and Schmidt, 2007). Forty players (mean age 15. 8 ± 0.96) were engaged in high performance centres and forty-two (mean age 15.6 ± 1.01) played in national level clubs. A decision tree and a random forest analysis between elite and national level players were performed. The most discriminant variable was Will to Excel, with 85,2% true positives in elite or national level. Mastery and competitiveness did not enter the final model. The will to reach excellence in performance can be considered as a condition to engage in more specialized and demanding practice. The assessment of the path to expertise only through motor variables or through the accumulated hours of deliberate practice is limited and can lead to mistaken identification or orientation of young sport talents. The use of a more comprehensive model is needed
108. Influence of coaches on sources of enjoyment in youth basketball
- Author
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Carvalho, Humberto M., Leonardi, Thiago J., Carlos Eduardo Gonçalves, and Paes, Roberto R.
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Maduración ,maturation ,education ,lcsh:BF1-990 ,Deporte joven ,athletes ,lcsh:Psychology ,Athletes ,Atleta ,youth sports ,Maturation ,multilevel modelling ,Modelos multinivel ,Multilevel modelling ,Youth sports ,human activities - Abstract
We examined the influence of coaches on sources of enjoyment of youth basketball players accounting for differences in chronological age, biological maturity status and years of training experience. Fifty-eight male basketball players aged 9.5 to 15.5 years were considered from a youth club. Three coaches supervising yearly age group teams from under 11 to under 15 teams were considered. Variables included chronological age, estimated age at peak height velocity, years of training experience, stature, body mass and sitting height by anthropometry; Sources of Enjoyment in Youth Sport Questionnaire was used. Multilevel modelling was used to partition variance between groups and control the influence of age and maturity status on enjoyment. Variance partition coefficients derived from multilevel null models showed a substantial variation by coach for age, maturity status and body dimensions. For the sources of enjoyment dimensions it was apparent an influence of the coach mostly for positive parental involvement. Controlling separately for age and somatic maturity status category, substantial variation by coach became apparent for self-referenced competencies and affiliation with peers, and the influence of coach was removed for positive parental involvement. After controlling variation associated to the coach the influence of age and maturity status remained present. The influence of the coach in the sources of enjoyment in adolescent basketball was apparent in adolescent basketball players. Furthermore, coaches' influence became more apparent after accounting for age and biological maturity status differences between players. El presente estudio investiga la influencia de los entrenadores en la satisfacción con la práctica deportiva en baloncesto juvenil, considerando las diferencias en la edad cronológica, maduración biológica y años de experiencia de entrenamiento. Los participantes (n = 58) de 9.5 a 15.5 años de un club de baloncesto juvenil participaron en el estudio. Tres entrenadores del club que conducirán equipas de sub-11 à sub-15 fueran considerados. Las variables incluyen la edad cronológica, edad estimada en el pico de crecimiento en la pubertad, años de experiencia de entrenamiento; estatura, masa corporal e altura sentado por antropometría; y el cuestionario Sources of Enjoyment in Youth Sport. Modelos multinivel fueran utilizados para investigar la variancia entre grupos y controlar el efecto de la edad y del estado de maduración en la satisfacción con la práctica deportiva. Los coeficientes de partición de la variancia resultantes de los modelos multinivel (modelos nulos) muestran una variación substancial cuando los jugadores están agrupados por entrenador para la edad, maduración biológica y tamaño corporal. En las dimensiones del Sources of Enjoyment in Youth Sport fue aparente una influencia del entrenador, sobretodo en el envolvimiento parental positivo. Controlando separadamente para la edad y de la maduración biológica, la influencia del entrenador se observa para las competencias auto referenciadas y para la afiliación con los pares, siendo removida la influencia del entrenador para el envolvimiento parental positivo. Después de controlar la variación asociada al entrenador la influencia de la edad e de la maduración biológica se mantenía. La influencia del entrenador en la satisfacción con la práctica deportiva en baloncesto juvenil fue aparente en lo presiente estudio, más cuando se controla la variación entre jugadores en la edad cronológica y la maduración biológica.
109. Why do they engage in such hard programs? The search for excellence in youth basketball.
- Author
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Gonçalves, Carlos E., e Silva, Manuel J. Coelho, Carvalho, Humberto M., and Gonçalves, Ângela
- Subjects
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TALENT management , *BASKETBALL for children , *EXPERTISE , *MOTIVATION (Psychology) in adolescence , *PHYSICAL training & conditioning , *BASKETBALL players , *PHYSIOLOGY - Abstract
Excellent performance in sport has a strong positive relationship with the accumulated hours of practice. The specialization years are seen as a decisive moment to lift the skill level, athletic readiness and commitment but the selection and orientation of talent has been strongly dependent of biological and motor variables. The purpose of this study is to describe the achievement and motivation variables that can explain the belonging to an elite competitive level of young basketball players. Eightytwo basketball players under 16 years fulfilled the WOFO Questionnaire (Spence and Helmreich, 1983), and an adapted version of the DPMQ (De Bruin, Rikers and Schmidt, 2007). Forty players (mean age 15.8 ± 0.96) were engaged in high performance centres and forty-two (mean age 15.6 ± 1.01) played in national level clubs. A decision tree and a random forest analysis between elite and national level players were performed. The most discriminant variable was Will to Excel, with 85,2% true positives in elite or national level. Mastery and competitiveness did not enter the final model. The will to reach excellence in performance can be considered as a condition to engage in more specialized and demanding practice. The assessment of the path to expertise only through motor variables or through the accumulated hours of deliberate practice is limited and can lead to mistaken identification or orientation of young sport talents. The use of a more comprehensive model is needed. [ABSTRACT FROM AUTHOR]
- Published
- 2011
110. Retrospective analysis of accumulated structured practice: A Bayesian multilevel analysis of elite Brazilian volleyball players.
- Author
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Mendes, Felipe G., Nascimento, Juarez V., Souza, Edison R., Collet, Carine, Milistetd, Michel, Côté, Jean, and Carvalho, Humberto M.
- Subjects
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VOLLEYBALL players , *PHYSICAL training & conditioning , *BAYESIAN analysis , *ADOLESCENCE , *VOLLEYBALL tournaments - Abstract
The patterns of cumulated structured volleyball practice and other structured sports activities of elite adult Brazilian players, considering age of specialization in volleyball and achievement of international competition representing the national team, were examined using Bayesian multilevel models. Elite volleyball players (n = 78) with an average age of 19.2 (SD = 0.9) years were considered. We used retrospective quantitative questionnaire to track individual training history. The mean age of specialization in volleyball was 10.7 (95% CI 10.3 to 11.0) for players that specialized early (before age 12), 14.1 (95% CI 13.9 to 14.3) for players that specialized intermediate (between ages 13 and 15), and 16.2 (95% CI 15.7 to 16.7) for players that specialized late (after age 16). Consequently, the earlier the specialization age in volleyball, the more years of training experience were accumulated. International and national level players were similar in both specialization age and pattern of engagement in other structured sport activities. Conditional on the data and models, attainment of expertise in volleyball may be favored by the accumulation of nonspecific sport experiences at early ages, and specialization may occur at a rather late age during adolescence. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
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111. Metabolic and Inflammatory Profiles in Children and Adolescents with Autism Spectrum Disorder: A Cross-Sectional Study.
- Author
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Gaspar JM, Ferreira JP, Carvalho HM, and Toscano CVA
- Abstract
Background/objectives: Autism spectrum disorder (ASD) is associated with several coexisting diseases or comorbidities, including inflammatory and metabolic disorders. In fact, ASD symptoms may be associated with immune system dysfunction. However, studies investigating the peripheral blood levels of immune cells are lacking and have provided mixed findings. In this study, we evaluated the relationship between the intensity level of ASD symptoms and the inflammatory and metabolic profiles in 154 children and adolescents (2-17 years)., Methods: Bayesian multilevel models were used to examine the relationship between their symptom intensities and inflammatory/metabolic profiles., Results: Heavier children had higher values for triglyceride and insulin levels. Children with a level 3 of ASD intensity had higher free fatty acids levels. However, when adjusting for ASD intensity, gender, medication use, or weight status, older children appeared to have higher values of triglycerides, insulin levels, and free fatty acids., Conclusions: We concluded that as Brazilian children with ASD became older, they had a higher risk for insulin resistance.
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- 2024
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112. Longitudinal Meta-Analysis of Peak Height Velocity in Young Female Athletes.
- Author
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Lima AB, Quinaud RT, Karasiak FC, Galvão LG, Gonçalves CE, and Carvalho HM
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Growth patterns and biological milestones in youth sports are key to interpreting the development of young athletes. However, there is no analysis of longitudinal meta-analysis describing the growth of young female athletes. This longitudinal meta-analysis estimated growth curves and age at peak height velocity (PHV) in young female athletes based on anthropometric data from longitudinal studies found in the literature. Following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, studies with repeated measurements in young female athletes were identified from searches of four databases (MEDLINE, Web of Science, SCOPUS, and SPORTDiscus) without date restrictions through August 2023. We adapted our bias assessment criteria using the Cochrane risk of bias tool for randomized controlled trials as a reference. Bayesian multilevel modeling was used to perform a longitudinal meta-analysis to extract stature growth curves and age at PHV. Fourteen studies met our eligibility criteria. Twenty-one independent samples could be included in the analysis. Conditional on the data and models, the predicted mean age at PHV for female athletes was 11.18 years (90% CI: 8.62; 12.94). When studies were aggregated by sport in the models, the models could not capture sport-specific growth curves for stature and estimate a corresponding age at PHV. We provide the first longitudinal meta-analytic summary of pubertal growth and derive age at PHV in young female athletes. The meta-analysis predicted that age at PHV occurs at similar ages to those in the general pediatric population. The data pool was limited in sports and geographic distribution, emphasizing the need to promote longitudinal research in females across different youth sports contexts., Competing Interests: The authors have declared that no competing interests exist., (Copyright © 2024, Lima et al.)
- Published
- 2024
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113. Determinants of drop-out in youth basketball: an interdisciplinary approach.
- Author
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Soares ALA, Kós LD, Paes RR, Nascimento JV, Collins D, Gonçalves CE, and Carvalho HM
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- Adolescent, Child, Humans, Male, Surveys and Questionnaires, Athletic Performance, Basketball, Body Size, Motivation
- Abstract
Using an interdisciplinary approach, we examined the baseline variation in biological maturity status, training experience, body size, functional capacities (Line Drill test and Yo-Yo Intermittent Recovery level 1 test) and motivation for achievement, competitiveness and deliberate practice of youth basketball players according to their participation status in the sport two years after assessment. Fifty-seven players were considered (10.5 to 15.5 years). Two years later we ascertained whether players discontinued participation (dropout), or remained playing engaged within a structured basketball training program. Taller adolescent players were more likely to be selected/promoted in youth basketball regardless of their lower functional capacity. Achievement and competitiveness motivation (will to excel and competitiveness) were related to dropping out or persisting in this sample of youth basketball players. Overall, there is a need to consider the interaction between physical growth, biological maturation, functional capacities and behavioural characteristics, specifically among players on the path to sport expertise.
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- 2020
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114. Growth and weight status of Brazilian children with autism spectrum disorders: A mixed longitudinal study.
- Author
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Toscano CVA, Ferreira JP, Gaspar JM, and Carvalho HM
- Subjects
- Adolescent, Bayes Theorem, Body Mass Index, Child, Child Development, Child, Preschool, Humans, Longitudinal Studies, Male, Obesity etiology, Overweight etiology, Autism Spectrum Disorder complications, Body Height
- Abstract
Objective: This study examined the growth status and physical development of Brazilian children with autism spectrum disorders from 4 to 15 years of age. Furthermore, it was examined whether variation in growth patterns and weight status was influenced by the use of psychotropic medications., Methods: One-hundred and twenty children aged 3.6-12.1 years at baseline (average=7.2 years, SD=2.3 years) diagnosed with autism spectrum disorders were measured on three repeated occasions across a 4-year period. Stature, body mass, and body mass index were considered. Bayesian multilevel modeling was used to describe the individual growth patterns., Results: Growth in stature was comparable to the age-specific 50th percentile for Centers for Disease Control and Prevention reference data until approximately 8 years, but a substantial decrease in growth rate was observed thereafter, reaching the age-specific 5th percentile at 15 years of age. Both body mass and body mass index values were, on average, higher than both the Brazilian and Centers for Disease Control and Prevention age-specific 95th percentile reference until 8 years, but below the 50th specific-age percentile at the age of 15 years., Conclusions: Brazilian boys with autism spectrum disorders between 4 and 15 years appear to have impaired growth in stature after 8-9 years of age, likely impacting pubertal growth. A high prevalence of overweight and obesity was observed in early childhood, although a trend of substantial decrease in body mass and body mass index was apparent when children with autism spectrum disorders entered the years of pubertal development., (Copyright © 2018 Sociedade Brasileira de Pediatria. Published by Elsevier Editora Ltda. All rights reserved.)
- Published
- 2019
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115. Sport selection in under-17 male roller hockey.
- Author
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Coelho-E-Silva MJ, Vaz V, Simões F, Carvalho HM, Valente-Dos-Santos J, Figueiredo AJ, Pereira V, Vaeyens R, Philippaerts R, Elferink-Gemser MT, and Malina RM
- Subjects
- Adolescent, Athletes, Body Height, Exercise, Fatigue, Hand Strength, Humans, Knee Joint, Male, Range of Motion, Articular, Athletic Performance, Hockey, Muscle Strength, Physical Education and Training, Physical Endurance, Physical Fitness
- Abstract
Characteristics of 32 international and 41 local under-17 (U-17) (14.5-16.5 years) roller hockey players were considered in the context of discrimination by competitive level using training history, anthropometry, skeletal maturation, and several laboratory and field performance tests. More international (42%) than local (22%) players were advanced in maturity status. International players had slightly less hockey experience (years), but had more practice sessions and match time (minutes) during the season. Local players were shorter and attained better performance in the 25-m dash, while international players performed better in sit-ups, ball throw and 20-m shuttle run. The fatigue index derived from the Wingate anaerobic test was higher among local players, while peak torques of knee extension and flexion were greater in international players. Stepwise discriminant function correctly classified 85% of players by competitive level based on grip strength, ratio of eccentric and concentric knee extension, number of training sessions, playing time and fatigue index. The results suggested an interaction among strength, anaerobic fitness and training plus game time as factors in discriminating international from local level players and by inference in the selection and development of youth roller hockey players.
- Published
- 2012
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116. Assessment of reliability in isokinetic testing among adolescent basketball players.
- Author
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Carvalho HM, Coelho E Silva MJ, Ronque ER, Gonçalves RS, Philippaerts RM, and Malina RM
- Subjects
- Adolescent, Exercise Test, Humans, Male, Reproducibility of Results, Athletes, Basketball physiology, Knee Joint physiology, Physical Fitness
- Abstract
Background: The reproducibility of day-to-day testing of isokinetic concentric and eccentric muscular actions among adolescent basketball players aged 14 to 16 years and relationships of mean within-subject variation in two isokinetic testing sessions with chronological age, biological maturation (estimated age at peak height velocity), training experience, body size, lower-body morphology, and initial strength performance were evaluated., Material and Methods: The sample included 27 basketball players who completed replicate test sessions of 5 repetitions of reciprocal concentric and eccentric knee extensions and flexions at 60º s(-1). A randomly selected subsample of 8 players completed a third testing session to confirm reliability estimates., Results: Coefficients of variation (CV) between sessions 1 and 2 ranged from 8.1% to 17.4%, and intraclass coefficients (ICCs) ranged from 0.72 to 0.89. For sessions 1 and 3, CVs ranged from 3.9% to 6.0%, and ICCs ranged from 0.95 to 0.99. The initial level of strength of eccentric knee flexion (r=-0.43) and eccentric knee extension (r=-0.42) were correlated (P<0.05) with eccentric knee extension within-variation between two sessions. Training experience (r=-0.37, P<0.05) and initial values of concentric knee flexion (r=-0.62, P<0.01) were correlated with concentric knee flexion within-subject differences. Within-subject variation of eccentric knee extension was correlated (P<0.05) with chronologic age (r=0.41), estimated age at peak height velocity (r=-0.38), body size (r=0.41 to 0.47), and leg volume (r=0.39)., Conclusions: Familiarization sessions may improve the reliability of concentric and eccentric knee isokinetic strength testing at 60º s(-1) in adolescent basketball players. Age, maturity status, and training experience of young athletes should be considered when testing knee isokinetic strength at 60º s(-1).
- Published
- 2011
117. Antibody against the carboxyl terminus of intimin alpha reduces enteropathogenic Escherichia coli adherence to tissue culture cells and subsequent induction of actin polymerization.
- Author
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Carvalho HM, Teel LD, Kokai-Kun JF, and O'Brien AD
- Subjects
- Adhesins, Bacterial chemistry, Animals, Binding Sites, Cells, Cultured, Escherichia coli Proteins chemistry, Flow Cytometry, Goats, Humans, Polymers metabolism, Actins metabolism, Adhesins, Bacterial immunology, Antibodies, Bacterial pharmacology, Bacterial Adhesion, Escherichia coli pathogenicity, Escherichia coli Proteins immunology
- Abstract
The C-terminal third of intimin binds to its translocated receptor (Tir) to promote attaching and effacing lesion formation during infection with enteropathogenic Escherichia coli (EPEC). We observed that the adherence of EPEC strains to HEp-2 cells was reduced and that actin polymerization was blocked by antibody raised against the C-terminal third of intimin alpha.
- Published
- 2005
- Full Text
- View/download PDF
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