101. Caveolae and caveolin-1 mediate endocytosis and transcytosis of oxidized low density lipoprotein in endothelial cells
- Author
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Xiaoyong Lei, Shao-wei Sun, Chao-Ke Tang, Lin-Xi Chen, Duan-Fang Liao, Qin-Hui Tuo, Xu-yu Zu, and Kai Li
- Subjects
Umbilical Veins ,Caveolin 1 ,Biology ,Caveolae ,Endocytosis ,Filipin ,Umbilical vein ,chemistry.chemical_compound ,Fluorescence microscope ,Humans ,Pharmacology (medical) ,Scavenger receptor ,Cells, Cultured ,Chromatography, High Pressure Liquid ,Pharmacology ,Endothelial Cells ,General Medicine ,Scavenger Receptors, Class E ,Lipoproteins, LDL ,Spectrometry, Fluorescence ,Microscopy, Fluorescence ,Biochemistry ,Transcytosis ,chemistry ,Biophysics ,Original Article ,lipids (amino acids, peptides, and proteins) - Abstract
To explore the mechanisms involved in ox-LDL transcytosis across endothelial cells and the role of caveolae in this process. An in vitro model was established to investigate the passage of oxidized low density lipoprotein (ox-LDL) through a tight monolayer of human umbilical vein endothelial cells (HUVEC) cultured on a collagen-coated filter. Passage of DiI-labeled ox-LDL through the monolayer was measured using a fluorescence spectrophotometer. The uptake and efflux of ox-LDL by HUVEC were determined using fluorescence microscopy and HPLC. Caveolae inhibitors – carrageenan (250 μg/mL), filipin (5 μg/mL), and nocodazole (33 μmol/L)–decreased the transport of ox-LDL across the monolayer by 48.9%, 72.4%, and 79.8% as compared to the control group. In addition, they effectively decreased ox-LDL uptake and inhibited the efflux of ox-LDL. Caveolin-1 and LOX-1 were up-regulated by ox-LDL in a time-dependent manner and decreased gradually after depletion of ox-LDL (P
- Published
- 2010