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The anatomical, physiological, and environmental changes of plants can alter the production of essential oil. The present study aims to characterize the profit and chemical constituents of Lavandula dentata L and Rosmarinus officinalis L. essential oils (EO), grown under conditions of stress by solar, water and soil radiation. A completely randomized design (CRD) was used in triplicate. The biomass analysis showed that in both plants, the best results were obtained when it was subjected to lighting, shading and 100% fertilization, but there was the lowest profit of EO. The portion that had the lowest biomass, showed the highest profit of EO. In the EO of Lavandula dentata L there were as major compounds: nerol, linalol and Myrcene, respectively, as the product with the lowest profit, eucalyptol stood out, followed by camphor and fenchone. The EO of Rosmarinus officinalis L with the lowest profit showed alpha-pinene as the major constituent, followed by camphor, 1.8 cineol, alpha-Terpinolene and linalol, and the EO with the highest profit was caryophyllene. The constituents of the EO showed different results when comparing the products obtained from the highest and lowest profit, both qualitatively and quantitatively, which shows that plants under different stress conditions affect the production of these secondary compounds, which may indicate differences in pharmacological actions.

Objetivo: asociar variables sociodemográficas con la autopercepción de los usuarios de la atención primaria de salud. Método: estudio transversal, con 222 usuarios de los servicios odontológicos de 15 unidades de atención primaria de un municipio de tamaño mediano de Rio Grande do Sul, Brasil. Se utilizaron cuestionarios con perfil sociodemográfico, la autopercepción y el Primary Care Assessment Tool Brasil salud bucal. Para comparaciones de las puntuaciones pruebas t-Student para grupos independientes y ANOVA (one way) – Post Hoc Sheffé. Resultados: usuarios con menor ingreso familiar (p=0,016) menor escolaridad (p=0,027) y que necesitaban hacer o cambiar la prótesis dental (p=0,020) obtuvieron asociación significativa en el atributo utilización de los servicios. En cuanto a la accesibilidad, las variables dolor de dientes (p=0,021) y satisfacción con la salud bucal (p

Objective: To associate sociodemographic variables with the self-perception of primary health care users. Method: Cross-sectional study with 222 users of dental services from 15 primary care units in a medium-sized city in Rio Grande do Sul, Brazil. Questionnaires with sociodemographic profile, self-perception, and the Primary Care Assessment Tool Brasil oral health were used. For comparisons of t-Student test scores for independent groups and ANOVA (one way) – Post Hoc Sheffé. Results: Users with lower family income (p=0.016), lower education (p=0.027), and who needed to make or replace dental prostheses (p=0.020) showed a significant association in the attribute of service utilization. Regarding accessibility, the variables toothache (p=0.021) and satisfaction with oral health (p

Objetivo: associar variáveis sociodemográficas com a autopercepção de usuários da atenção primária à saúde. Método: estudo transversal, com 222 usuários dos serviços odontológicos de 15 unidades de atenção primária de município de médio porte do Rio Grande do Sul, Brasil. Foram utilizados questionários com perfil sociodemográfico, a autopercepção e o Primary Care Assessment Tool Brasil saúde bucal. Para comparações dos escores testes t-Student para grupos independentes e ANOVA (one way) – Post Hoc Sheffé. Resultados: usuários com menor renda familiar (p=0,016) menor escolaridade (p=0,027) e que precisavam fazer ou trocar a prótese dentária (p=0,020) obtiveram associação significativa no atributo utilização dos serviços. Quanto à acessibilidade, as variáveis dor de dente (p=0,021) e satisfação com a saúde bucal (p

The objective were to evaluate the effects of supplementation of standardized dry extract of Rosmarinus officinalis (RO) and the application of aesthetic radiofrequency on the oxidative stress markers catalase (CAT), superoxide dismutase (SOD), non-protein thiols (NP-SH), and thiobarbituric acid reactive species (TBARS) and the biochemical markers triglycerides, total cholesterol, high density lipoprotein (HDL) cholesterol, glutamic-oxaloacetic transaminase (TGO/AST), pyruvic-glutamic transaminase (TGP/ALT), gamma glutamyl transpeptidase (gamma-GT), and creatinine. This study included 32 women received the aesthetic therapy to reduce localized fat. They were divided into the control group (n = 8) receiving placebo capsules and the intervention group (n = 24) subdivided into Group A, B, and C, each with eight members receiving supplementation with 100, 500, and 1000 mg/day of standardized dry extract of RO, respectively. The Universal Trial Number (UTN) – U1111-1274-6255. Supplementation with RO (500 mg/day) demonstrated a reduction in oxidative stress (quantified with through a significant increase in NP-SH and a reduction in SOD and CAT enzymes). The radiofrequency aesthetic treatment did not promote an increase in oxidative stress; however, it caused significant changes in total cholesterol, HDL cholesterol, and creatinine. RO is a plant with antioxidant effects and its oral consumption is safe in selected women subjects in hepatic and renal markers.

Impact and visibility are important boosters for global scientific publishing in English regarding some possible tensions. We interviewed four Brazilian medical researchers from five well-ranked universities in Brazil through semi-structured interviews. We used linguistic ethnography theorizing as a methodology to analyze the social relations around texts, based on literacy studies. The aim of this article is to discuss Brazilian medical authors’ experienced tensions regarding publishing internationally and writing in English. Results showed that writing in English to publish internationally is a literacy practice embedded in power relationships and legitimation. We identified three main tensions: 1) a low number of Brazilian English speakers which results in poor access to written English skills; 2) handling relations of power shaping discourses and standards; and 3) issues tied to academic group support and academic literacy.

BACKGROUND AND OBJECTIVES: During the COVID-19 pandemic, health professionals have experienced a work overload that may be associated with increased pain intensity. The aim of this study was to analyze the frequency and intensity of musculoskeletal pain in the different anatomical regions reported by health professionals who worked in Intensive Care Units (ICU) during the pandemic and to investigate associations between clinical variables. METHODS: This is a cross-sectional, analytical, quantitative and multicenter study carried out in six ICU between July 2021 and February 2022. The Nordic Questionnaire of Musculoskeletal Symptoms (QNSO) was used for data collection, as well as a questionnaire prepared by the researchers on sociodemographic, work and clinical data, and the visual numeric scale (VNS) for pain assessment. Analyses were carried out using descriptive and inferential statistics. RESULTS: The sample consisted of 205 health professionals. With regard to pain intensity, according to the visual analog scale (VAS), an average of 3.76 was observed. The analysis of the association between the variables indicated a greater intensity of pain in females, with a higher frequency of mild and moderate pain, age over 40, in the professional categories of nursing technicians and doctors. The presence of severe pain was associated with poor general health and shorter time in professional practice. CONCLUSION: Most of the professionals reported pain of varying intensity and in different anatomical regions, the most frequently cited being the upper back and neck. Musculoskeletal disorders are directly related to work activity, age, gender and lack of leisure time.

Abstract Sub-Saharan Africa is under-represented in global biodiversity datasets, particularly regarding the impact of land use on species’ population abundances. Drawing on recent advances in expert elicitation to ensure data consistency, 200 experts were convened using a modified-Delphi process to estimate ‘intactness scores’: the remaining proportion of an ‘intact’ reference population of a species group in a particular land use, on a scale from 0 (no remaining individuals) to 1 (same abundance as the reference) and, in rare cases, to 2 (populations that thrive in human-modified landscapes). The resulting bii4africa dataset contains intactness scores representing terrestrial vertebrates (tetrapods: ±5,400 amphibians, reptiles, birds, mammals) and vascular plants (±45,000 forbs, graminoids, trees, shrubs) in sub-Saharan Africa across the region’s major land uses (urban, cropland, rangeland, plantation, protected, etc.) and intensities (e.g., large-scale vs smallholder cropland). This dataset was co-produced as part of the Biodiversity Intactness Index for Africa Project. Additional uses include assessing ecosystem condition; rectifying geographic/taxonomic biases in global biodiversity indicators and maps; and informing the Red List of Ecosystems.

Studies show that two specific mutations result in approximately 40% of alleles for glycogen-storage diseases. Through cloning a cDNA that encoded a putative glucose-6-phosphate translocase, researchers determined the mutations that resulted in disease alleles. These findings could indicate that glucose-6-phosphate and inorganic phosphate are transported by the same transporter in microsomes.

A novel method for the synthesis of (vinylstannyl)cyclopentylamine derivatives was developed based on the tributyltin hydride-mediated cycloisomerization of allene-tethered oxime ethers or hydrazones. The synthetic strategy involves the site-directed intermolecular attack of the tributyltin radical at the allene moiety of allene-tethered oxime ethers. The reactions also involved the acid-promoted destannylation of the resulting vinyltin intermediates to produce unsaturated cyclopentylamine derivatives.

Introduction: Aesthetic radiofrequency treatment is a therapeutic method focused on reducing adiposity, of reducing body weight, concomitant with the use of aesthetic techniques using herbal medicines and medicinal plants. One of the plants still little explored by health professionals is Rosmarinus officinalis. Objective: To evaluate the impact of aesthetic radiofrequency treatment, combined with the use of standardized dry extract R. officinalis, on anthropometric variables in healthy women. Materials and Methods: Thirty-two healthy women were subjected to radiofrequency cosmetic treatment for 4 weeks. These women were divided into Control Group (CG) and Intervention Group (IG). In CG (n=8), placebo capsules were included in the diet. The intervention group was divided into: Group A (n=8) 100 mg day-1; Group B (n=8) 500 mg day-1; Group C (n=8): 1,000 mg day-1. Anthropometric data regarding abdominal skinfolds, abdominal circumferences, and bioimpedance were collected before and after the interventions. Results: The aesthetic treatment with radiofrequency did not change the parameters evaluated. Significant changes were observed for the use of R. officinalis, through the significant increase in skinfolds, for all doses evaluated, as well as reduction in abdominal circumference with the use of 500 mg. Conclusion: Aesthetic radiofrequency treatment did not result in significant changes in anthropometric variables. Oral supplementation with R. officinalis extract can increase abdominal skinfolds in a linear, dose-dependent way. In addition, oral supplementation can significantly change abdominal circumferences.

Abstract The limited sensitivity of circulating tumor cell (CTC) detection in pancreatic adenocarcinoma (PDAC) stems from their extremely low concentration in the whole circulating blood, necessitating enhanced detection methodologies. This study sought to amplify assay-sensitivity by employing diagnostic leukapheresis (DLA) to screen large blood volumes. Sixty patients were subjected to DLA, with a median processed blood volume of ~ 2.8 L and approximately 5% of the resulting DLA-product analyzed using CellSearch (CS). Notably, DLA significantly increased CS-CTC detection to 44% in M0-patients and 74% in M1-patients, yielding a 60-fold increase in CS-CTC enumeration. DLA also provided sufficient CS-CTCs for genomic profiling, thereby delivering additional genomic information compared to tissue biopsy samples. DLA CS-CTCs exhibited a pronounced negative prognostic impact on overall survival (OS), evidenced by a reduction in OS from 28.6 to 8.5 months (univariate: p = 0.002; multivariable: p = 0.043). Additionally, a marked enhancement in sensitivity was achieved (by around 3-4-times) compared to peripheral blood (PB) samples, with positive predictive values for OS being preserved at around 90%. Prognostic relevance of CS-CTCs in PDAC was further validated in PB-samples from 228 PDAC patients, consolidating the established association between CTC-presence and reduced OS (8.5 vs. 19.0 months, p

Abstract Approximately 10% of antimicrobials used by humans in low- and middle-income countries are estimated to be substandard or falsified. In addition to their negative impact on morbidity and mortality, they may also be important drivers of antimicrobial resistance. Despite such concerns, our understanding of this relationship remains rudimentary. Substandard and falsified medicines have the potential to either increase or decrease levels of resistance, and here we discuss a range of mechanisms that could drive these changes. Understanding these effects and their relative importance will require an improved understanding of how different drug exposures affect the emergence and spread of resistance and of how the percentage of active pharmaceutical ingredients in substandard and falsified medicines is temporally and spatially distributed.

Bioavailability assessment is important for evaluating the risks to the local biota, and the combined use of several ecological risk indices in eutrophic environments allows the best analysis of the local reality for decision-making. The relationship between acid volatile sulfide (AVS) concentrations and simultaneously extracted metals (SEM) allows us to infer the metal holding capacity of sediment, with the objective of evaluating the potential bioavailability of trace metals (Cd, Cu, Ni, Pb, and Zn) using ecological risk indices, such as the ΣSEM/AVS model and Adverse Effect Index (AEI), in surface sediments from Guanabara Bay and Sepetiba Bay, Brazil. AVS was determined using a colorimetric method and SEM with ICP-OES. In general, almost all sampling in Sepetiba Bay showed ΣSEM/AVS ratio values above 1. However, all results for the ΣSEM/AVS ratio found for the Guanabara Bay sediments were

Salmonella Paratyphi A, one of the major etiologic agents of enteric fever, has increased in prevalence in recent decades in certain endemic regions in comparison to S. Typhi, the most prevalent cause of enteric fever. Despite this increase, data on the prevalence and molecular epidemiology of S. Paratyphi A remain generally scarce. Here, we analysed the whole genome sequences of 216 S. Paratyphi A isolates originating from Kathmandu, Nepal between 2005 and 2014, of which 200 were from patients with acute enteric fever and 16 from the gallbladder of people with suspected chronic carriage. By exploiting the recently developed genotyping framework for S. Paratyphi A (Paratype), we identified several genotypes circulating in Kathmandu. Notably, we observed an unusual clonal expansion of genotype 2.4.3 over a four-year period that spread geographically and systematically replaced other genotypes. This rapid genotype replacement is hypothesised to have been driven by both reduced susceptibility to fluoroquinolones and genetic changes to virulence factors, such as functional and structural genes encoding the type 3 secretion systems. Finally, we show that person-to-person is likely the most common mode of transmission and chronic carriers seem to play a limited role in maintaining disease circulation.

Esta resenha aborda a obra "Cidadãos Substituídos por Algoritmos" de Néstor García Canclini, cujo objetivo é demonstrar que, no estado atual, o uso de redes sociais em massa, com consciência e engajamento político, funciona como uma nova forma de exercício da cidadania. Ao longo do livro, o autor dialoga sobre a maneira pela qual a mobilização pelas redes já propiciaram importantes embates revolucionários neste século. Dessa forma, a obra apresenta uma perspectiva bastante positiva do uso da tecnologia, sendo ela capaz, inclusive, de formatar novas formas de participação social que estão além da mobilização partidária.

Objectives The aim of this study was to map and compare stakeholders’ perceptions of barriers towards cervical cancer screening for vulnerable women in seven European countries.Design In Collaborative User Boards, stakeholders were invited to participate to identify barriers towards participation in cervical cancer screening.Setting The study is nested in the European Union-funded project CBIG-SCREEN which aims to tackle inequity in cervical cancer screening for vulnerable women (www.cbig-screen.eu). Data collection took place in Bulgaria, Denmark, Estonia, France, Italy, Portugal and Romania.Participants Participants represented micro-level stakeholders covering representatives of users, that is, vulnerable women, meso-level stakeholders covering healthcare professionals and social workers, and macro-level stakeholders covering programme managers and decision-makers.Methods Across the seven countries, 25 meetings in Collaborative User Boards with a duration of 2 hours took place between October 2021 and June 2022. The meetings were video recorded or audio recorded, transcribed and translated into English for a qualitative framework analysis.Results 120 participants took part in the Collaborative User Boards. Context-specific barriers were related to different healthcare systems and characteristics of vulnerable populations. In Romania and Bulgaria, the lack of a continuous screening effort and lack of ways to identify eligible women were identified as barriers for all women rather than being specific for women in vulnerable situations. The participants in Denmark, Estonia, France, Italy and Portugal identified providers’ lack of cultural and social sensitivity towards vulnerable women as barriers. In all countries, vulnerable women’s fear, shame and lack of priority to preventive healthcare were identified as psychological barriers.Conclusion The study provides an overview of stakeholders’ perceived barriers towards vulnerable women’s cervical cancer screening participation in seven European countries. The organisation of healthcare systems and the maturity of screening programmes differ between countries, while vulnerable women’s psychological barriers had several similarities.

Objetivo: analisar a dor musculoesquelética em profissionais de saúde na atenção primária e sua vivência durante a pandemia da COVID-19. Método: estudo de métodos mistos, realizado no sul do Brasil, entre junho de 2021 e fevereiro de 2022. Amostra de 50 participantes, utilizando os questionários relacionados à dor e aos sintomas osteomusculares e entrevistas com roteiros pré-elaborados. Análise de dados com estatísticas descritivas, inferenciais e Análise Temática de Conteúdo. Resultados: a maior parte dos participantes foram da área da Enfermagem, com 40 horas semanais. Regiões mais acometidas: ombros, parte superior das costas e pescoço, variando de dor moderada (42%) e intensa (10%). Diferenças estatísticas significativas relacionadas à dor e medo de contrair a COVID-19, entre aqueles com problemas prévios de saúde e enfermeiros. Conclusão: tendo em vista que a dor pode estar associada às condições de trabalho, torna-se essencial a identificação dos fatores de risco, evitando maiores agravos para a saúde do trabalhador.

Objective: To analyze musculoskeletal pain in primary care health professionals and their experience during the COVID-19 pandemic. Method: A mixed-methods study carried out in southern Brazil between June 2021 and February 2022. A sample of 50 participants, using questionnaires related to pain and musculoskeletal symptoms and interviews with pre-prepared scripts. Data analysis using descriptive and inferential statistics and Thematic Content Analysis. Results: Most of the nursing sector participants worked 40 hours a week. The most affected areas are the shoulders, upper back, and neck, ranging from moderate (42%) to severe (10%) pain. Statistically significant differences related to pain and fear of contracting COVID-19 between those with previous health problems and nurses. Conclusion: Given that pain can be associated with working conditions, it is essential to identify risk factors to avoid further damage to workers' health.

Objetivo: Analizar el dolor musculoesquelético en los profesionales de la salud de atención primaria y sus experiencias durante la pandemia de COVID-19. Método: estudio de métodos mixtos realizado en el sur de Brasil entre junio de 2021 y febrero de 2022. Una muestra de 50 participantes, mediante cuestionarios relacionados con el dolor y los síntomas musculoesqueléticos y entrevistas con guiones preparados de antemano. Análisis de datos mediante estadísticas descriptivas e inferenciales y análisis temático de contenido. Resultados: La mayoría de las participantes pertenecían al sector de la enfermería y trabajaban 40 horas semanales. Regiones más afectadas: hombros, parte superior de la espalda y cuello, con dolores que van de moderados (42%) a intensos (10%). Diferencias estadísticamente significativas relacionadas con el dolor y el miedo a contraer COVID-19 entre las personas con problemas de salud previos y las enfermeras. Conclusión: Dado que el dolor puede estar asociado a las condiciones de trabajo, es esencial identificar los factores de riesgo y evitar mayores daños a la salud de los trabajadores.

Abstract RNAseq data can be used to infer genetic variants, yet its use for estimating genetic population structure remains underexplored. Here, we construct a freely available computational tool (RGStraP) to estimate RNAseq-based genetic principal components (RG-PCs) and assess whether RG-PCs can be used to control for population structure in gene expression analyses. Using whole blood samples from understudied Nepalese populations and the Geuvadis study, we show that RG-PCs had comparable results to paired array-based genotypes, with high genotype concordance and high correlations of genetic principal components, capturing subpopulations within the dataset. In differential gene expression analysis, we found that inclusion of RG-PCs as covariates reduced test statistic inflation. Our paper demonstrates that genetic population structure can be directly inferred and controlled for using RNAseq data, thus facilitating improved retrospective and future analyses of transcriptomic data.