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101. MLVA-16 typing of 295 marine mammal Brucella isolates from different animal and geographic origins identifies 7 major groups within Brucella ceti and Brucella pinnipedialis

Author

Jacques Isabelle, Al Dahouk Sascha, Djønne Berit, Ramisse Françoise, Tryland Morten, Foster Geoffrey, Le Flèche Philippe, Maquart Marianne, Neubauer Heinrich, Walravens Karl, Godfroid Jacques, Cloeckaert Axel, and Vergnaud Gilles

Subjects
Microbiology, QR1-502
Abstract

Abstract Background Since 1994, Brucella strains have been isolated from a wide range of marine mammals. They are currently recognized as two new Brucella species, B. pinnipedialis for the pinniped isolates and B. ceti for the cetacean isolates in agreement with host preference and specific phenotypic and molecular markers. In order to investigate the genetic relationships within the marine mammal Brucella isolates and with reference to terrestrial mammal Brucella isolates, we applied in this study the Multiple Loci VNTR (Variable Number of Tandem Repeats) Analysis (MLVA) approach. A previously published assay comprising 16 loci (MLVA-16) that has been shown to be highly relevant and efficient for typing and clustering Brucella strains from animal and human origin was used. Results 294 marine mammal Brucella strains collected in European waters from 173 animals and a human isolate from New Zealand presumably from marine origin were investigated by MLVA-16. Marine mammal Brucella isolates were shown to be different from the recognized terrestrial mammal Brucella species and biovars and corresponded to 3 major related groups, one specific of the B. ceti strains, one of the B. pinnipedialis strains and the last composed of the human isolate. In the B. ceti group, 3 subclusters were identified, distinguishing a cluster of dolphin, minke whale and porpoise isolates and two clusters mostly composed of dolphin isolates. These results were in accordance with published analyses using other phenotypic or molecular approaches, or different panels of VNTR loci. The B. pinnipedialis group could be similarly subdivided in 3 subclusters, one composed exclusively of isolates from hooded seals (Cystophora cristata) and the two others comprising other seal species isolates. Conclusion The clustering analysis of a large collection of marine mammal Brucella isolates from European waters significantly strengthens the current view of the population structure of these two species, and their relative position with respect to the rest of the Brucella genus. MLVA-16 is confirmed as being a rapid, highly discriminatory and reproducible method to classify Brucella strains including the marine mammal isolates. The Brucella2009 MLVA-16 genotyping database available at http://mlva.u-psud.fr/ is providing a detailed coverage of all 9 currently recognized Brucella species.

Published
2009
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102. DNA polymorphism analysis of Brucella lipopolysaccharide genes reveals marked differences in O-polysaccharide biosynthetic genes between smooth and rough Brucella species and novel species-specific markers

Author

Cloeckaert Axel, Letesson Jean-Jacques, Blasco José M, Zygmunt Michel S, and Moriyón Ignacio

Subjects
Microbiology, QR1-502
Abstract

Abstract Background The lipopolysaccharide is a major antigen and virulence factor of Brucella, an important bacterial pathogen. In smooth brucellae, lipopolysaccharide is made of lipid A-core oligosaccharide and N-formylperosamine O-polysaccharide. B. ovis and B. canis (rough species) lack the O-polysaccharide. Results The polymorphism of O-polysaccharide genes wbkE, manAO-Ag, manBO-Ag, manCO-Ag, wbkF and wbkD) and wbo (wboA and wboB), and core genes manBcore and wa** was analyzed. Although most genes were highly conserved, species- and biovar-specific restriction patterns were found. There were no significant differences in putative N-formylperosamyl transferase genes, suggesting that Brucella A and M serotypes are not related to specific genes. In B. pinnipedialis and B. ceti (both smooth), manBO-Ag carried an IS711, confirming its dispensability for perosamine synthesis. Significant differences between smooth and rough species were found in wbkF and wbkD, two adjacent genes putatively related to bactoprenol priming for O-polysaccharide polymerization. B. ovis wbkF carried a frame-shift and B. canis had a long deletion partially encompassing both genes. In smooth brucellae, this region contains two direct repeats suggesting the deletion mechanism. Conclusion The results define species and biovar markers, confirm the dispensability of manBO-Ag for O-polysaccharide synthesis and contribute to explain the lipopolysaccharide structure of rough and smooth Brucella species.

Published
2009
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103. Salmonella Agona harboring genomic island 1-A

Author

Doublet, Benoit, Butaye, Patrick, Imberechts, Hein, Collard, Jean-Marc, Chaslus-Dancla, Elisabeth, and Cloeckaert, Axel

Subjects
Salmonella
Abstract

To the Editor: Multidrug-resistant Salmonella enterica serovar Typhimurium definitive phage type 104 has emerged during the 1980s and 1990s as a world health problem because of its implications in animal [...]

Published
2004

105. Experimental and theoretical evaluation of typing methods based upon random amplification of genomic restriction fragments (AFLP) for bacterial population genetics

Author

Mougel, Christophe, Teyssier, Sylvie, d’Angelo, Cathy, Groud, Karine, Neyra, Mare, Sidi-Boumedine, Karim, Cloeckaert, Axel, Peloille, Michèle, Baucheron, Sylvie, Chaslus-Dancla, Élisabeth, Jarraud, Sophie, Meugnier, Hélène, Forey, Françoise, Vandenesch, François, Lina, Gérard, Étienne, Jéröme, Thioulouse, Jean, Manceau, Charles, Robbe, Patrick, Nalin, Renaud, Briolay, Jéröme, and Nesme, Xavier

Published
2001
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107. Extended-spectrum β-lactamase-encoding genes are spreading on a wide range of Escherichia coli plasmids existing prior to the use of third-generation cephalosporins

Author

Ledda, Alice, Billard-Pomares, Typhaine, Doublet, Benoît, Fouteau, Stéphanie, Barbe, Valérie, Roche, David, Cruveiller, Stéphane, Médigue, Claudine, Castellanos, Miguel, Decré, Dominique, Drieux-Rouze, Laurence, Clermont, Olivier, Glodt, Jérémy, Tenaillon, Olivier, Cloeckaert, Axel, Arlet, Guillaume, Denamur, Erick, and Branger, Catherine

Subjects
Escherichia coli, plasmid, extended-spectrum β-lactamase, CTX-M-15, gene-sharing network, Microbiology and Parasitology, gène codant, plasmide, analyse génomique, escherichia coli, Microbiologie et Parasitologie, beta lactamase
Abstract

To understand the evolutionary dynamics of extended-spectrum β-lactamase (ESBL)-encoding genes in Escherichia coli, we undertook a comparative genomic analysis of 116 whole plasmid sequences of human or animal origin isolated over a period spanning before and after the use of third-generation cephalosporins (3GCs) using a gene-sharing network approach. The plasmids included 82 conjugative, 22 mobilizable and 9 non-transferable plasmids and 3 P-like bacteriophages. ESBL-encoding genes were found on 64 conjugative, 6 mobilizable, 2 non-transferable plasmids and 2 P1-like bacteriophages, indicating that these last three types of mobile elements also play a role, albeit modest, in the diffusion of the ESBLs. The network analysis showed that the plasmids clustered according to their genome backbone type, but not by origin or period of isolation or by antibiotic-resistance type, including type of ESBL-encoding gene. There was no association between the type of plasmid and the phylogenetic history of the parental strains. Finer scale analysis of the more abundant clusters IncF and IncI1 showed that ESBL-encoding plasmids and plasmids isolated before the use of 3GCs had the same diversity and phylogenetic history, and that acquisition of ESBL-encoding genes had occurred during multiple independent events. Moreover, the blaCTX-M-15 gene, unlike other CTX-M genes, was inserted at a hot spot in a blaTEM-1-Tn2 transposon. These findings showed that ESBL-encoding genes have arrived on wide range of pre-existing plasmids and that the successful spread of blaCTX-M-15 seems to be favoured by the presence of well-adapted IncF plasmids that carry a Tn2-blaTEM-1 transposon.

Published
2018

108. Scientific Reports

Author

Al Dahouk, Sascha, Köhler, Stephan, Occhialini, Alessandra, Jiménez de Bagüés, María Pilar, Hammerl, Jens Andre, Eisenberg, Tobias, Vergnaud, Gilles, Cloeckaert, Axel, Zygmunt, Michel, Whatmore, Adrian, Melzer, Falk, Drees, Kevin, Foster, Jeffrey, Wattam, Alice, Scholz, Holger, Department of Biological Safety, Bundesinstitut für Risikobewertung - Federal Institute for Risk Assessment (BfR), Institut de Recherche en Infectiologie de Montpellier (IRIM), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Unidad de Producción y Sanidad Animal, Centro de Investigación y Tecnología Agroalimentaria, Instituto Agroalimentario de Aragón, University of Zaragoza - Universidad de Zaragoza [Zaragoza], Landesbetrieb Hessisches Landeslabor, Institut de génétique et microbiologie [Orsay] (IGM), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Infectiologie Animale et Santé Publique - IASP (Nouzilly, France), Institut National de la Recherche Agronomique (INRA), German National Reference Laboratory for Animal Brucellosis, Federal Research Institute for Animal Health - Friedrich-Loeffler-Institut, Northern Arizona University [Flagstaff], Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Centre d’études d’Agents Pathogènes et Biotechologies pour la Santé (CPBS), Université Paris Sud (Paris 11), Centre National de la Recherche Scientifique (CNRS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Université Paris-Saclay, Animal and Plant Health Agency (APHA), Department of Molecular, Cellular, and Biomedical Sciences, University of New Hampshire (UNH), Biocomplexity Institute of Virginia Tech [Blacksburg], Virginia Tech [Blacksburg], Bundeswehr Institute of Microbiology, Federal funds from National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services HHSN272201400027C, Spanish grant INIA-RTA2013-00065-C02-01, Federal Institute for Risk Assessment, Germany 1329-485, Food and Rural Affairs at German Federal Institute SE0314 SE0316, Department of Environment, Federal Ministry of Food and Agriculture 47-003 1322-503 1322-619, Hessian Ministry of Environment, Climate Change, Agriculture and Consumer Protection, French DGA (Direction Generale de l'Armement) via the MicroType project ANR-14-ASMA-0002-02, European Defence Agency (EDA), Université de Montpellier (UM), UR Infectiologie animale et Santé publique (UR IASP), Bundeswehr Institute of Microbiology, Department of Bacteriology, Munich, Germany, RWTH Aachen University, Centro de Investigacion y Tecnologia Agroalimentaria de Aragon (CITA), Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Animal and Plant Health Agency [Addlestone, UK] (APHA), Friedrich-Loeffler-Institut (FLI), German Center for Infection Research, Partnersite Munich (DZIF), Rheinisch-Westfälische Technische Hochschule Aachen University (RWTH), Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), and Institut National de la Recherche Agronomique (INRA)-Université de Tours

Subjects
phylogénétique, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, [SDV]Life Sciences [q-bio], analysis resource, murine models, Tanzania, Mice, Germany, phénotypage, GeneralLiterature_REFERENCE(e.g.,dictionaries,encyclopedias,glossaries), Transmisión de enfermedades, Phylogeny, Mice, Inbred BALB C, amphibians, monocyte-derived macrophages, lipopolysaccharide, comparative sequence, mammifère, genetic diversity, Brucelosis, Biological Evolution, Brucellaceae, bacterial bioinformatics database, melitensis 16m, vole microtus-arvalis, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Liver, Flagella, Host-Pathogen Interactions, Anura, Autre (Sciences du Vivant), [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], Biotechnologies, macrophage, Anfibios, brucella spp, Article, Cell Line, Genetic Heterogeneity, Bacterial Proteins, death, Animals, mammals, analyse moléculaire, Mamíferos, Macrophages, amphibien, Gene Expression Regulation, Bacterial, Brucella, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, infection, Producción y sanidad animal, Animals, Zoo, souche bactérienne, Gram-Negative Bacterial Infections, Spleen, Multilocus Sequence Typing
Abstract

Scientific reports 7, 44420 (2017). doi:10.1038/srep44420, Published by Nature Publishing Group, London

Published
2017
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109. QnrD in fluoroquinolone-resistant Proteae of animal origin

Author

Schultz, Eliette, Baucheron-Monnier, Sylvie, Doublet, Benoît, Cloeckaert, Axel, Madec, Jean-Yves, Haenni, Marisa, Infectiologie Animale et Santé Publique - IASP (Nouzilly, France), Institut National de la Recherche Agronomique (INRA), Unité Antibiorésistance et Virulence Bactériennes, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, University of Veterinary Medicine [Hannover]. DEU., and Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT)

Subjects
carbohydrates (lipids), enzyme, antibiothérapie, [SDV]Life Sciences [q-bio], education, bacteria, protéase, biochemical phenomena, metabolism, and nutrition, fluoroquinolone, humanities
Abstract

QnrD in fluoroquinolone-resistant [i]Proteae[/i] of animal origin. 7. Symposium on Antimicrobial Résistance in Animals and the Environment (ARAE)

Published
2017

114. WadD, a New Brucella Lipopolysaccharide Core Glycosyltransferase Identified by Genomic Search and Phenotypic Characterization

Author

Salvador-Bescós, Miriam, primary, Gil-Ramírez, Yolanda, additional, Zúñiga-Ripa, Amaia, additional, Martínez-Gómez, Estrella, additional, de Miguel, María J., additional, Muñoz, Pilar M., additional, Cloeckaert, Axel, additional, Zygmunt, Michel S., additional, Moriyón, Ignacio, additional, Iriarte, Maite, additional, and Conde-Álvarez, Raquel, additional

Published
2018
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116. B rucella

Author

Scholz, Holger C., primary, Banai, Menachem, additional, Cloeckaert, Axel, additional, Kämpfer, Peter, additional, and Whatmore, Adrian M., additional

Published
2018
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117. Multidrug resistance Salmonella genomic island 1 in a Morganella morganii subsp. morganii human clinical isolate from France

Author

Schultz, Eliette, Barraud, Olivier, Madec, Jean-Yves, Haenni, Marisa, Cloeckaert, Axel, Ploy, Marie-Cécile, Doublet, Benoît, Infectiologie Animale et Santé Publique - IASP (Nouzilly, France), Institut National de la Recherche Agronomique (INRA), Unité Antibiorésistance et Virulence Bactériennes, Laboratoire de Lyon, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Université de Lyon, Anti-infectieux : supports moléculaires des résistances et innovations thérapeutiques (RESINFIT), CHU Limoges-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST), Université de Limoges (UNILIM)-Université de Limoges (UNILIM), Service de Bactériologie, Virologie, Hygiène [CHU Limoges], CHU Limoges, French National Institute of Agricultural Research, Ministère de l’Enseignement Supérieur et de la Recherche, Institut National de la Santé et de la Recherche Médicale (Inserm), Anses, INRA, Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Unité Antibiorésistance et Virulence Bactériennes (AVB), Laboratoire de Lyon [ANSES], Université de Lyon-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université de Lyon-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), ProdInra, Archive Ouverte, and Institut National de la Recherche Agronomique (INRA)-Université de Tours

Subjects
integrative mobilizable element, Microbiology and Parasitology, Observation, Salmonella genomic island 1, Microbiology, QR1-502, Microbiologie et Parasitologie, Clinical Science and Epidemiology, séquençage du génome, proteus mirabilis, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Salmonella genomic island, integrons, multidrug resistance, bacteria, salmonella enterica, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology
Abstract

Since its initial identification in epidemic multidrug-resistant Salmonella enterica serovar Typhimurium DT104 strains, several SGI1 variants, SGI1 lineages, and SGI1-related elements (SGI2, PGI1, and AGI1) have been described in many bacterial genera (Salmonella, Proteus, Morganella, Vibrio, Shewanella, etc.). They constitute a family of multidrug resistance site-specific integrative elements acquired by horizontal gene transfer, SGI1 being the best-characterized element. The horizontal transfer of SGI1/PGI1 elements into other genera is of public health concern, notably with regard to the spread of critically important resistance genes such as ESBL and carbapenemase genes. The identification of SGI1 in Morganella morganii raises the issue of (i) the potential for SGI1 to emerge in other human pathogens and (ii) its bacterial host range. Further surveillance and research are needed to understand the epidemiology, the spread, and the importance of the members of this SGI1 family of integrative elements in contributing to antibiotic resistance development., Salmonella genomic island 1 (SGI1) is a multidrug resistance integrative mobilizable element that harbors a great diversity of antimicrobial resistance gene clusters described in numerous Salmonella enterica serovars and also in Proteus mirabilis. A serious threat to public health was revealed in the recent description in P. mirabilis of a SGI1-derivative multidrug resistance island named PGI1 (Proteus genomic island 1) carrying extended-spectrum-β-lactamase (ESBL) and metallo-β-lactamase resistance genes, blaVEB-6 and blaNDM-1, respectively. Here, we report the first description of Salmonella genomic island 1 (SGI1) in a multidrug-resistant clinical Morganella morganii subsp. morganii strain isolated from a patient in France in 2013. Complete-genome sequencing of the strain revealed SGI1 variant SGI1-L carrying resistance genes dfrA15, floR, tetA(G), blaPSE-1 (now referred to as blaCARB-2), and sul1, conferring resistance to trimethoprim, phenicols, tetracyclines, amoxicillin, and sulfonamides, respectively. The SGI1-L variant was integrated into the usual chromosome-specific integration site at the 3′ end of the trmE gene. Beyond Salmonella enterica and Proteus mirabilis, the SGI1 integrative mobilizable element may thus also disseminate its multidrug resistance phenotype in another genus belonging to the Proteae tribe of the family Enterobacteriaceae. IMPORTANCE Since its initial identification in epidemic multidrug-resistant Salmonella enterica serovar Typhimurium DT104 strains, several SGI1 variants, SGI1 lineages, and SGI1-related elements (SGI2, PGI1, and AGI1) have been described in many bacterial genera (Salmonella, Proteus, Morganella, Vibrio, Shewanella, etc.). They constitute a family of multidrug resistance site-specific integrative elements acquired by horizontal gene transfer, SGI1 being the best-characterized element. The horizontal transfer of SGI1/PGI1 elements into other genera is of public health concern, notably with regard to the spread of critically important resistance genes such as ESBL and carbapenemase genes. The identification of SGI1 in Morganella morganii raises the issue of (i) the potential for SGI1 to emerge in other human pathogens and (ii) its bacterial host range. Further surveillance and research are needed to understand the epidemiology, the spread, and the importance of the members of this SGI1 family of integrative elements in contributing to antibiotic resistance development.

Published
2017
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118. SGI1, BLSE et AmpC chez des Proteus spp d’origine animale

Author

Schultz, Eliette, Doublet, Benoît, Cloeckaert, Axel, Jean-Yves, Madec, Haenni, Marisa, Infectiologie Animale et Santé Publique - IASP (Nouzilly, France), Institut National de la Recherche Agronomique (INRA), Unité Antibiorésistance et Virulence Bactériennes, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Association de Chimiothérapie Anti-Infectieuse (ACAI). FRA., and Institut National de la Recherche Agronomique (INRA)-Université de Tours

Subjects
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, proteus, pathogenic bacteria, animal, bactérie pathogène
Abstract

SGI1, BLSE et AmpC chez des Proteus spp d’origine animale. 36. Réunion Interdisciplinaire de Chimiothérapie Anti-Infectieuse (RICAI 2016)

Published
2016

119. What's in a Name? Species-Wide Whole-Genome Sequencing Resolves Invasive and Noninvasive Lineages of Salmonella enterica Serotype Paratyphi B

Author

Connor, Thomas R, Owen, Sian V, Langridge, Gemma, Connell, Steve, Nair, Satheesh, Reuter, Sandra, Dallman, Timothy J, Corander, Jukka, Tabing, Kristine C, Le Hello, Simon, Fookes, Maria, Doublet, Benoît, Zhou, Zhemin, Feltwell, Theresa, Ellington, Matthew, Herrera, Silvia, Gilmour, Matthew, Cloeckaert, Axel, Achtman, Mark, Parkhill, Julian, Wain, John, de Pinna, Elizabeth, Weill, François-Xavier, Peters, Tansy, Thomson, Nick, The Wellcome Trust Sanger Institute [Cambridge], Cardiff University, University of Liverpool, Public Health England [London], University of Helsinki, Public Health Agency of Canada [Winnipeg], Centre National de Référence - National Reference Center Escherichia coli, Shigella et Salmonella (CNR-ESS), Institut Pasteur [Paris], Université de Tours, Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, University of Warwick [Coventry], Instituto de Salud Carlos III [Madrid] (ISC), Norwich Medical School, University of East Anglia [Norwich] (UEA), University of Oslo (UiO), This work, including the efforts of Thomas R. Connor, Gemma Langridge,Theresa Feltwell, Julian Parkhill, and Nicholas Thomson, wasfunded by Wellcome Trust (098051). This work, including the effortsof Thomas R. Connor, was funded by Medical Research Council(MRC) (MR/L015080/1).F.-X.W. and S.L. are funded by the Institut Pasteur, the Institut de VeilleSanitaire, and the French Government’s Investissement d’Avenir programIntegrative Biology of Emerging Infectious Diseases Laboratory ofExcellence (grant number ANR-10-LABX-62-IBEID).The funders had no role in study design, data collection and interpretation,or the decision to submit the work for publication, ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), Centre National de Référence des Escherichia coli, Shigella et Salmonella - Bactéries pathogènes entériques (CNR-ESS), Infectiologie Animale et Santé Publique - IASP (Nouzilly, France), Institut National de la Recherche Agronomique (INRA), Instituto de Salud Carlos III (ISC), ANR-10-LABX-62-IBEID,IBEID,Laboratoire d'Excellence 'Integrative Biology of Emerging Infectious Diseases'(2010), Wellcome Trust, Medical Research Council (Reino Unido), Université de Tours (UT), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Department of Mathematics and Statistics, Jukka Corander / Principal Investigator, Biostatistics Helsinki, Helsingin yliopisto = Helsingfors universitet = University of Helsinki, and Institut Pasteur [Paris] (IP)

Subjects
PROVIDES, Genotype, OUTBREAK, SEROVAR PARATYPHI, UNITED-STATES, Sequence Analysis, DNA, Microbiology, EVOLUTION, QR1-502, QR, Salmonella paratyphi B, SURVEILLANCE, 1181 Ecology, evolutionary biology, Paratyphoid Fever, Animals, Cluster Analysis, Humans, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, POPULATION-STRUCTURE, TYPHIMURIUM, RESISTANCE, 1183 Plant biology, microbiology, virology, Genome, Bacterial, JAVA, Research Article
Abstract

For 100 years, it has been obvious that Salmonella enterica strains sharing the serotype with the formula 1,4,[5],12:b:1,2—now known as Paratyphi B—can cause diseases ranging from serious systemic infections to self-limiting gastroenteritis. Despite considerable predicted diversity between strains carrying the common Paratyphi B serotype, there remain few methods that subdivide the group into groups that are congruent with their disease phenotypes. Paratyphi B therefore represents one of the canonical examples in Salmonella where serotyping combined with classical microbiological tests fails to provide clinically informative information. Here, we use genomics to provide the first high-resolution view of this serotype, placing it into a wider genomic context of the Salmonella enterica species. These analyses reveal why it has been impossible to subdivide this serotype based upon phenotypic and limited molecular approaches. By examining the genomic data in detail, we are able to identify common features that correlate with strains of clinical importance. The results presented here provide new diagnostic targets, as well as posing important new questions about the basis for the invasive disease phenotype observed in a subset of strains., IMPORTANCE Salmonella enterica strains carrying the serotype Paratyphi B have long been known to possess Jekyll and Hyde characteristics; some cause gastroenteritis, while others cause serious invasive disease. Understanding what makes up the population of strains carrying this serotype, as well as the source of their invasive disease, is a 100-year-old puzzle that we address here using genomics. Our analysis provides the first high-resolution view of this serotype, placing strains carrying serotype Paratyphi B into the wider genomic context of the Salmonella enterica species. This work reveals a history of disease dating back to the middle ages, caused by a group of distinct lineages with various abilities to cause invasive disease. By quantifying the key genomic differences between the invasive and noninvasive populations, we are able to identify key virulence-related targets that can form the basis of simple, rapid, point-of-care tests.

Published
2016
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120. Antibiotic stress and dynamics of evolution and expression of antibiotic resistance genetic elements (ASTRE)

Author

Jové, Thomas, Baucheron, Sylvie, Cloeckaert, Axel, Le Hello, Simon, Ploy, Marie-Cecile, Doublet, Benoît, Institut National de la Santé et de la Recherche Médicale (INSERM), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Institut Pasteur [Paris] (IP), Fédération de recherche en infectiologie (FéRI)., Institut National de la Recherche Agronomique (INRA)-Université de Tours, and Institut Pasteur [Paris]

Subjects
antibiotic resistance, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Salmonella, SOS, ComputingMilieux_MISCELLANEOUS
Abstract

Session 3 - Thérapies et résistance; National audience

Published
2016

121. Characterisation of fluoroquinolone resistance mechanisms in multidrug‐resistant Salmonella Brandenburg isolated from patients from a same hospital in France

Author

Baucheron, Sylvie, Monchaux, Isabelle, Cloeckaert, Axel, Le Hello, Simon, Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Institut Pasteur [Paris], and Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT)

Subjects
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2016

122. Identification of a Toxin‐Antitoxin System on Salmonella Genomic Island 1and Incompatibility with IncA/C Plasmids

Author

Huguet, Kévin, Cloeckaert, Axel, Doublet, Benoît, Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, and Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT)

Subjects
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2016

123. Mobilization functions required for the conjugative transfer of Salmonella Genomic Island 1

Author

Hegyi, Anna, Szabó, Mónika, Nagy, Istvan, Douard, Gregory, Doublet, Benoît, Cloeckaert, Axel, Kiss, János, National Agricultural Research and Innovation Center (NARIC), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, and Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT)

Subjects
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2016

124. Brucella vulpis sp. nov., a novel Brucella species isolated from mandibular lymph nodes of red foxes (Vulpes vulpes) in Austria

Author

Hofer , Erwin, Hammerl , Jens A., Zygmunt , Michel S., Cloeckaert , Axel, Koylass , Mark, Whatmore , Adrian M., Blom , Jochen, Revilla-Fernández , Sandra, Witte , Angela, Scholz , Holger C., Vergnaud , Gilles, Al Dahouk , Sascha, Aistleitner , Karin, WHO/FAO/OIE, Austrian Agency for Health and Food Safety (AGES), Central Institute of the Bundeswehr Medical Service, Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Bundesinstitut für Risikobewertung - Federal Institute for Risk Assessment (BfR), Federal Institute for Risk Assessment (BfR project no. 47-003, 1322-503, and 1322-619). Robert Koch-Institut (RKI) on behalf of the German Bundesministerium für Gesundheit (BMG), grant FKZ 1369-448, Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Center for Biotechnology (CeBiTec), Universität Bielefeld = Bielefeld University, Max Perutz Labs (MFPL), Medizinische Universität Wien = Medical University of Vienna-University of Vienna [Vienna], Institut de Biologie Intégrative de la Cellule ( I2BC ), Université Paris-Sud - Paris 11 ( UP11 ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université Paris-Saclay-Centre National de la Recherche Scientifique ( CNRS ), and Federal Institute for Risk Assessment

Subjects
[ SDV.MP.BAC ] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
Abstract

International audience; Two slow-growing, Gram-negative, non-motile, non-spore-forming, coccoid bacteria (strains F60T and F965), isolated in Austria from mandibular lymph nodes of two red foxes (Vulpes vulpes), were subjected to a polyphasic taxonomic analysis. In a recent study, both isolates were assigned to the genus Brucella but could not be attributed to any of the existing species. Hence, we have analysed both strains in further detail to determine their exact taxonomic position and genetic relatedness to other members of the genus Brucella. The genome sizes of F60T and F965 were 3 236 779 and 3 237 765 bp, respectively. Each genome consisted of two chromosomes, with a DNA G+C content of 57.2 %. A genome-to-genome distance of >80 %, an average nucleotide identity (ANI) of 97 % and an average amino acid identity (AAI) of 98 % compared with the type species Brucella melitensis confirmed affiliation to the genus. Remarkably, 5 % of the entire genetic information of both strains was of non-Brucella origin, including as-yet uncharacterized bacteriophages and insertion sequences as well as ABC transporters and other genes of metabolic function from various soil-living bacteria. Core-genome-based phylogenetic reconstructions placed the novel species well separated from all hitherto-described species of the genus Brucella, forming a long-branched sister clade to the classical species of Brucella. In summary, based on phenotypic and molecular data, we conclude that strains F60T and F965 are members of a novel species of the genus Brucella, for which the name Brucella vulpis sp. nov. is proposed, with the type strain F60T ( = BCCN 09-2T = DSM 101715T).

Published
2016
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125. Updating the Brucella MLVA database

Author

Vergnaud, G., Hauck, Yolande, Pourcel, Christine, Jacques, Isabelle, Cloeckaert, Axel, Zygmunt, Michel, Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Bundeswehr Institute of Microbiology. DEU., I2BC, Centre National de la Recherche Scientifique (CNRS), ProdInra, Migration, and Institut National de la Recherche Agronomique (INRA)-Université de Tours

Subjects
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, ComputingMilieux_MISCELLANEOUS
Abstract

Session K : Microbial Population Genomics; International audience

Published
2016

126. A novel Brucella species isolated from a dog in Costa Rica

Author

Zygmunt, Michel, Gonnet, Mathieu, Scholz, H.C., Vergnaud, G., Cloeckaert, Axel, Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Bundeswehr Institute of Microbiology, I2BC, Centre National de la Recherche Scientifique (CNRS), Bundeswehr Institute of Microbiology. DEU., Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), and ProdInra, Migration

Subjects
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, ComputingMilieux_MISCELLANEOUS
Abstract

Session DP : Biological Protection and Environmental Hazards - Poster Presentations; International audience

Published
2016

127. What's in a Name? Species-Wide Whole-Genome Sequencing Resolves Invasive and Noninvasive Lineages of Salmonella enterica Serotype Paratyphi B

Author

Owen, Sian V, Langridge, Gemma, Connell, Steve, Nair, Satheesh, Reuter, Sandra, Dallman, Timothy J, Corander, Jukka, Tabing, Kristine C, Le Hello, Simon, Fookes, Maria, Doublet, Benoît, Zhou, Zhemin, Feltwell, Theresa, Ellington, Matthew J, Herrera, Silvia, Gilmour, Matthew, Cloeckaert, Axel, Achtman, Mark, Parkhill, Julian, Wain, John, De Pinna, Elizabeth, Weill, François-Xavier, Peters, Tansy, Thomson, Nick, and Connor, Thomas R

Subjects
séquence génomique, Microbiology and Parasitology, sérotype, population invasive, salmonella enterica, Microbiologie et Parasitologie
Abstract

For 100 years, it has been obvious that Salmonella enterica strains sharing the serotype with the formula 1,4,[5],12:b:1,2-now known as Paratyphi B-can cause diseases ranging from serious systemic infections to self-limiting gastroenteritis. Despite considerable predicted diversity between strains carrying the common Paratyphi B serotype, there remain few methods that subdivide the group into groups that are congruent with their disease phenotypes. Paratyphi B therefore represents one of the canonical examples in Salmonella where serotyping combined with classical microbiological tests fails to provide clinically informative information. Here, we use genomics to provide the first high-resolution view of this serotype, placing it into a wider genomic context of the Salmonella enterica species. These analyses reveal why it has been impossible to subdivide this serotype based upon phenotypic and limited molecular approaches. By examining the genomic data in detail, we are able to identify common features that correlate with strains of clinical importance. The results presented here provide new diagnostic targets, as well as posing important new questions about the basis for the invasive disease phenotype observed in a subset of strains.[br/] Salmonella enterica strains carrying the serotype Paratyphi B have long been known to possess Jekyll and Hyde characteristics; some cause gastroenteritis, while others cause serious invasive disease. Understanding what makes up the population of strains carrying this serotype, as well as the source of their invasive disease, is a 100-year-old puzzle that we address here using genomics. Our analysis provides the first high-resolution view of this serotype, placing strains carrying serotype Paratyphi B into the wider genomic context of the Salmonella enterica species. This work reveals a history of disease dating back to the middle ages, caused by a group of distinct lineages with various abilities to cause invasive disease. By quantifying the key genomic differences between the invasive and noninvasive populations, we are able to identify key virulence-related targets that can form the basis of simple, rapid, point-of-care tests.

Published
2016

128. Dominant plasmids carrying extended spectrum β-lactamases blaCTX-M genes in genetically diverse Escherichia coli from slaughterhouse and urban wastewaters

Author

Dupouy-Guiraute, Véronique, Doublet, Benoît, Arpaillange, Nathalie, Praud, Karine, Bibbal, Delphine, Brugère, Hubert, Oswald, Eric, Cloeckaert, Axel, Toutain, Pierre-Louis, Bousquet‐Mélou, Alain, ToxAlim (ToxAlim), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Institut de Recherche en Santé Digestive (IRSD ), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Fédérale Toulouse Midi-Pyrénées, CHU Toulouse [Toulouse], ProdInra, Migration, Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), and Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)

Subjects
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, ESBL-producing E. coli, plasmid, Escherichia coli, blaCTX-M, slaughterhouse, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, wastewater, WWTP
Abstract

International audience; Wastewater treatment plants (WWTP) receiving effluents from food-producing animals and humans may contribute to the spread of ESBL-carrying plasmids. This study was designed to investigate extended-spectrum cephalosporin resistant E. coli strains, CTX-M distributions and the genetic lineage of blaCTX-M -carrying plasmids from urban and slaughterhouse wastewaters. The level of extended-spectrum cephalosporin-resistant E. coli in slaughterhouse wastewater entering the WWTP was negligible compared to that of urban wastewater. The blaCTX-M-1 gene was predominant in slaughterhouse wastewater whereas diverse blaCTX-M genes were encountered in urban wastewater and WWTP outlet. Characterization of the main CTX-M-producing E. coli isolates by antibiotic resistance phenotyping, genotyping and typing of plasmids carrying blaCTX-M genes revealed that blaCTX-M-1 and blaCTX-M-15 genes were harbored by the predominant blaCTX-M-1 IncI1/ST3 and blaCTX-M-15 F31:A4:B1 plasmids, which were recovered from unrelated E. coli genotypes in both slaughterhouse and urban wastewaters. This study highlighted the spread of predominant blaCTX-M-1 and blaCTX-M-15 plasmid lineages in diverse E. coli genotypes from humans and food-producing animals, their mixing in WWTP and final release into the aquatic environment. This could have a serious negative impact on public health and requires further evaluation.

Published
2016
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131. Brucella Genetic Variability in Wildlife Marine Mammals Populations Relates to Host Preference and Ocean Distribution

Author

Suárez-Esquivel, Marcela, primary, Baker, Kate S., additional, Ruiz-Villalobos, Nazareth, additional, Hernández-Mora, Gabriela, additional, Barquero-Calvo, Elías, additional, González-Barrientos, Rocío, additional, Castillo-Zeledón, Amanda, additional, Jiménez-Rojas, César, additional, Chacón-Díaz, Carlos, additional, Cloeckaert, Axel, additional, Chaves-Olarte, Esteban, additional, Thomson, Nicholas R., additional, Moreno, Edgardo, additional, and Guzmán-Verri, Caterina, additional

Published
2017
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132. Brucella spp. of amphibians comprise genomically diverse motile strains competent for replication in macrophages and survival in mammalian hosts

Author

Al Dahouk, Sascha, primary, Köhler, Stephan, additional, Occhialini, Alessandra, additional, Jiménez de Bagüés, María Pilar, additional, Hammerl, Jens Andre, additional, Eisenberg, Tobias, additional, Vergnaud, Gilles, additional, Cloeckaert, Axel, additional, Zygmunt, Michel S., additional, Whatmore, Adrian M., additional, Melzer, Falk, additional, Drees, Kevin P., additional, Foster, Jeffrey T., additional, Wattam, Alice R., additional, and Scholz, Holger C., additional

Published
2017
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134. A heterogeneous population of motile Brucellae out of the frog pond

Author

Al-Dahouk, S., Kohler, S., Occhialini, A., Jiménez de Bagüés, María P, Hammerl, Jens A, Eisenberg, Tobias, Vergnaud, G., Cloeckaert, Axel, Zygmunt, Michel S., Whatmore, Adrian M., Melzer, Falk, Drees, K.P., Foster, J.T., Wattam, A.R., Scholz, Holger C., Department of Biological Safety, Bundesinstitut für Risikobewertung - Federal Institute for Risk Assessment (BfR), Centre d’études d’Agents Pathogènes et Biotechologies pour la Santé (CPBS), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), University of Zaragoza - Universidad de Zaragoza [Zaragoza], Landesbetrieb Hessisches Landeslabor, I2BC, Centre National de la Recherche Scientifique (CNRS), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Animal and Plant Health Agency [Addlestone, UK] (APHA), Friedrich-Loeffler-Institut (FLI), Department of Molecular, Cellular, and Biomedical Sciences, University of New Hampshire (UNH), Virginia Bioinformatics Institute, Virginia Tech [Blacksburg], Bundeswehr Institute of Microbiology, ProdInra, Migration, Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), and Bundeswehr Institute of Microbiology. DEU.

Subjects
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Rana, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, Brucella, ComputingMilieux_MISCELLANEOUS
Abstract

Session DP : Biological Protection and Environmental Hazards; International audience

Published
2015

135. Insertion sequences in bacterial genomes: a route towards stepwise cumulative multiple antibiotic resistance acquisition?

Author

Doublet, Benoît, Baucheron, Sylvie, Jové, Thomas, Barraud, Olivier, Giraud, Etienne, Cloeckaert, Axel, Ploy, Marie-Cecile, Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Université de Limoges (UNILIM), ProdInra, Migration, and Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT)

Subjects
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology
Abstract

National audience; The overall objective of this project proposal is to understand how the acquisition of mobile genetic elements (MGE) by bacteria may represent a key event in the evolution towards multidrug resistance (MDR), especially for mutation-mediated resistances accumulating upon antibiotic exposure. It is well known that plasmids and genomic islands represent hot-spots for accessory genes acquisition (insertion sequences (IS), resistance and/or virulence genes), however their collateral effects on the genetic host background are fairly unknown. We hypothesize that the acquisition of mobile genetic elements has been the key event in fluoroquinolone (FQ) resistance evolution by the introduction of IS elements. The objective of our proposal is to assess the importance of IS introduction into genomes on the accumulation of resistance mutations. P. mirabilis is implicated in several catheter-associated urinary tract infections, and in respiratory or bloodstream infections. P. mirabilis has also the ability to acquire and maintain MDR-carrying mobile genetic elements and to become resistant to FQs. Our hypothesis is that a positive correlation exists between the MDR evolution (FQ resistance mutations accumulation) and IS expansion in the chromosome. To determine this, a first approach will be to analyze the expansion of IS elements in regard to stepwise FQ resistance mutations accumulation in MDR clinical strains using P. mirabilis as a model. Secondly we propose to determine which bacterial functions are affected (positively/negatively) by the transposition of IS elements. Moreover, we plan to investigate to which extent some IS contribute to the expression of low-level quinolone resistance genes, qnrA which have been found associated to ISCR1. The possible interplay between IS transposition and the induction of the SOS response in regard to antimicrobial resistance evolution, in particular FQ resistance mutations accumulation, will be assessed experimentally in vitro. The inhibition of the SOS response has been proposed as a mean to reduce generation of antibiotic-resistant mutants and could represent an innovative strategy to fight antimicrobial resistance.

Published
2015

136. Identification of mutations in the ram locus of fluoroquinolones resistant Salmonella enterica serogroup Choleraesuis isolates

Author

Baucheron, Sylvie, Chiu, Cheng-Hsun, Chu, Chishih, Cloeckaert, Axel, Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Chang Gung Memorial Hospital [Taipei] (CGMH), National Chiayi University, and Institut National de la Recherche Agronomique (INRA)-Université de Tours

Subjects
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2015

137. A Toxin-Antitoxin system on SGI1 and incompatibility with IncA/C plasmids

Author

Huguet, Kévin, Doublet, Benoît, Cloeckaert, Axel, Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, and Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT)

Subjects
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2015

138. Crystal structure of multidrug resistance regulator RamR complexed with bile acids

Author

Yamasaki, Suguru, Nakashima, Ryosuke, Sakurai, Keisuke, Baucheron, Sylvie, Giraud, Etienne, Doublet, Benoît, Cloeckaert, Axel, Nishino, Kunihiko, Osaka University, Institute of Scientific and Industrial Research, Osaka University [Osaka], Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, and Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT)

Subjects
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2015

139. Influence of biliary salts and of the ramRA locus on Salmonella Typhimurium invasion

Author

Giraud, Etienne, Baucheron, Sylvie, Doublet, Benoît, Monchaux, Isabelle, Nishino, Kunihiko, Cloeckaert, Axel, Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Institute of Scientific and Industrial Research, Osaka University [Osaka], and Institut National de la Recherche Agronomique (INRA)-Université de Tours

Subjects
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2015

140. A specific SGI1 multidrug resistance region in ciprofloxacin-resistant Salmonella enterica serotype Kentucky from human and poultry sources in Belgium

Author

Doublet, Benoît, Ceyssens, Pieter-Jan, Jasson, Vicky, Mattheus, Wesley, Praud, Karine, Butaye, Patrick, Wattiau, Pierre, Vanhoof, Raymond, Bertrand, Sophie, Cloeckaert, Axel, Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Scientific Institute for Public Health, Sciensano [Bruxelles], Réseau International des Instituts Pasteur (RIIP), and Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT)

Subjects
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2015

141. Brucella spp. of amphibians comprise genomically diverse motile strains competent for replication in macrophages and survival in mammalian hosts

Author

Al Dahouk, Sascha, Koehler, Stephan, Occhialini, Alessandra, Jimenez de Bagues, Maria Pilar, Hammerl, Jens Andre, Eisenberg, Tobias, Vergnaud, Gilles, Cloeckaert, Axel, Zygmunt, Michel S., Whatmore, Adrian M., Melzer, Falk, Drees, Kevin P., Foster, Jeffrey T., Wattam, Alice R., Scholz, Holger C., Al Dahouk, Sascha, Koehler, Stephan, Occhialini, Alessandra, Jimenez de Bagues, Maria Pilar, Hammerl, Jens Andre, Eisenberg, Tobias, Vergnaud, Gilles, Cloeckaert, Axel, Zygmunt, Michel S., Whatmore, Adrian M., Melzer, Falk, Drees, Kevin P., Foster, Jeffrey T., Wattam, Alice R., and Scholz, Holger C.

Abstract

Twenty-one small Gram-negative motile coccobacilli were isolated from 15 systemically diseased African bullfrogs (Pyxicephalus edulis), and were initially identified as Ochrobactrum anthropi by standard microbiological identification systems. Phylogenetic reconstructions using combined molecular analyses and comparative whole genome analysis of the most diverse of the bullfrog strains verified affiliation with the genus Brucella and placed the isolates in a cluster containing B. inopinata and the other non-classical Brucella species but also revealed significant genetic differences within the group. Four representative but molecularly and phenotypically diverse strains were used for in vitro and in vivo infection experiments. All readily multiplied in macrophage-like murine J774-cells, and their overall intramacrophagic growth rate was comparable to that of B. inopinata BO1 and slightly higher than that of B. microti CCM 4915. In the BALB/c murine model of infection these strains replicated in both spleen and liver, but were less efficient than B. suis 1330. Some strains survived in the mammalian host for up to 12 weeks. The heterogeneity of these novel strains hampers a single species description but their phenotypic and genetic features suggest that they represent an evolutionary link between a soil-associated ancestor and the mammalian host-adapted pathogenic Brucella species.

Published
2017
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142. A heterogeneous population of motile brucellae out of the frog pond

Author

Al Dahouk, Sascha, Köhler, Stephan, Occhialini, Alessandra, Jiménez de Bagüés Picazo, María Pilar, Hammerl, Jens André, Vergnaud, Gilles, Cloeckaert, Axel, Zygmunt, Michael Stanislas, Whatmore, Adrian M., Melzer, Falk, Wattam, Alice R., Drees, Kevin P., and Foster, Jeffrey T.

Subjects
Rana, Brucella
Published
2015

143. Un système toxine/antitoxine impliqué dans la stabilité de SGI1

Author

Huguet, Kévin, Cloeckaert, Axel, Doublet, Benoît, Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), ProdInra, Migration, and Institut National de la Recherche Agronomique (INRA)-Université de Tours

Subjects
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], [SDV.OT] Life Sciences [q-bio]/Other [q-bio.OT], système toxine-antitoxine, SGI1, vecteurs d'expression, ComputingMilieux_MISCELLANEOUS, stabilité génomique
Abstract

National audience

Published
2014

146. Brucella vulpis sp. nov., isolated from mandibular lymph nodes of red foxes (Vulpes vulpes)

Author

Scholz, Holger C., primary, Revilla-Fernández, Sandra, additional, Dahouk, Sascha Al, additional, Hammerl, Jens A., additional, Zygmunt, Michel S., additional, Cloeckaert, Axel, additional, Koylass, Mark, additional, Whatmore, Adrian M., additional, Blom, Jochen, additional, Vergnaud, Gilles, additional, Witte, Angela, additional, Aistleitner, Karin, additional, and Hofer, Erwin, additional

Published
2016
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147. Incompatibility between Salmonella genomic island 1 and IncA/C plasmids

Author

Huguet, Kévin, Cloeckaert, Axel, Doublet, Benoît, Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, and Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT)

Subjects
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2013

148. Emergence of Multidrug Resistance Genomic Island SGI1 in Proteus mirabilis in Humans and Animals in France

Author

Schultz, Eliette, Madec, Jean-Yves, Haenni, Marisa, Cloeckaert, Axel, Doublet, Benoît, Unité Antibiorésistance et Virulence Bactériennes, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Bundeswehr Institute of Microbiology. DEU., and Institut National de la Recherche Agronomique (INRA)-Université de Tours

Subjects
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2013

149. Genotypic Expansion within the Population Structure of Classical Brucella Species Revealed by MLVA-16 Typing of 1414 Brucella Isolates from Different Animal and Geographic Origins, 1974-2006

Author

Zygmunt, Michel S., Pourcel, Christine, Hauck, Yolande, Jacques, Isabelle, Vergnaud, Gilles, Cloeckaert, Axel, Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), ProdInra, Migration, and Institut National de la Recherche Agronomique (INRA)-Université de Tours

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2013

150. A toxin-antitoxin system on Salmonella Genomic Island 1 ?

Author

Huguet, Kévin, Doublet, Benoît, Cloeckaert, Axel, Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Université François Rabelais (Tours). Tours, FRA., Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), and ProdInra, Migration

Subjects
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2013

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