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The Fusarium incarnatum-equiseti species complex (FIESC) is shown to encompass 33 phylogenetic species, across a wide range of habitats/hosts around the world. Here, 77 pathogenic and endophytic FIESC strains collected from China were studied to investigate the phylogenetic relationships within FIESC, based on a polyphasic approach combining morphological characters, multi-locus phylogeny and distribution patterns. The importance of standardised cultural methods to the identification and classification of taxa in the FIESC is highlighted. Morphological features of macroconidia, including the shape, size and septum number, were considered as diagnostic characters within the FIESC. A multi-locus dataset encompassing the 5.8S nuclear ribosomal gene with the two flanking internal transcribed spacers (ITS), translation elongation factor (EF-1a), calmodulin (CAM), partial RNA polymerase largest subunit (RPB1) and partial RNA polymerase second largest subunit (RPB2), was generated to distinguish species within the FIESC. Nine novel species were identified and described. The RPB2 locus is demonstrated to be a primary barcode with high success rate in amplification, and to have the best species delimitation compared to the other four tested loci. [ABSTRACT FROM AUTHOR]

[Purpose/Significance] The online interaction of participatory websites improves the efficiency of information transmission and communication, and improves users' perception and experience. Through the study of bullet screen under participatory website, it complements the relevant research on user experience theory, and provides practical guidance for short video disseminators and relevant participatory websites. [Method/Process] This paper selected Bilibili, a typical participatory website, as the research object, took the short video bullet screen data and aggregated hotness indicator data of Bilibili as the research samples to study its user interaction experience, used python to crawl bullet screen popularity and content information of short videos, and studied its users’ aggregated hotness indicators and user generated contents of participatory websites through correlation analysis and text emotion analysis. [Result/Conclusion] The results show that there is a correlation between the number of bullet screen, the number of comments and the number of the video playback of participatory websites; Real time bullet screen interaction enhances the audience's cognitive sharing, emotional experience and onlooker experience.

Huge hazards are frequently caused by earthquake-induced rock avalanche. The study of dynamic response characteristics and failure mechanism of the rock slope in specific geological condition is a challenging issue in geotechnical engineering. In this paper, a shaking table test that reproduces the Shuiqiuchi rock avalanche was carried out to understand the dynamic response and failure mechanism of rock slope controlled by faults. The testing results show that when the dip angle of the fault is greater than a specific critical angle, part of the reflected and transmitted waves at the discontinuous interface change into sliding waves, resulting in a sudden change in the acceleration response at the fault. The peak acceleration amplification factor inside the model slope presents a significant three-stage trend. Peak horizontal acceleration amplification factor increases obviously with elevation, while peak vertical acceleration amplification factor increases slightly with elevation. The natural frequency curve of the slope model can be divided into three stages with a downward trend, which indicates that the dynamic characteristics of the model have changed. By comparing the shaking table test with the Shuiqiuchi rock avalanche prototype, the main failure mode of the rock slope with fault structure is found as follows: the slope crest first shows vertical tensile cracks under the seismic load, followed by cracking damage of the fragmented rock mass in the hanging wall of the fault, and finally sliding occurs along the fault surface. This research could provide references for the early-warning of granite avalanche, and offer the basic data and scientific support for the development of Qinling Mountain geological heritages.

ObjectiveThis study aimed to investigate a new noninvasive traction method on the treatment of severe cervical kyphotic deformity.MethodsThe clinical data of patients with severe cervical kyphosis (Cobb > 40°) treated in Peking University Third Hospital from March 2004 to March 2020 were retrospectively summarized. 46 cases were enrolled, comprising 27 males and 19 females. Fifteen patients underwent skull traction, and 31 patients underwent suspensory traction. Among them, seven used combined traction after one week of suspensory traction. Bedside lateral radiographs were taken every two or three days during traction. The cervical kyphosis angle was measured on lateral radiographs in and extended position at each point in time. The correction rate and evaluated Japanese Orthopedic Association (JOA) scoring for the function of the spinal cord were also measured. The data before and after the operation were compared with paired sample t-test or Wilcoxon signed-rank test.ResultsNo neurological deterioration occurred during the skull traction and the cervical suspensory traction. There were 12 patients with normal neurological function, and the JOA score of the other 34 patients improved from 11.5 ± 2.8 to 15.4 ± 1.8 at the end of follow up (P

Takayasu arteritis (TA) is a chronic granulomatous vasculitis involving in the main branches of aorta. Previous studies mainly used peripheral blood and some vascular tissues but seldom studies have sequenced vascular tissues. Here in this study, we aimed to explore the alterations of mRNA in TA by performing bulk RNA sequencing. A total of 14 abdominal aortic tissues including 8 from renal transplantation and 6 from patient with TA undergoing bypass surgeries. Bulk RNA sequencing were performed and after the quality control, a total of 1897 transcripts were observed to be significantly differently (p < 0.05 and Log2FC > 1) expressed between the TA and control group, among which 1,361 transcripts were in TA group and 536 in the Control group. Reactome Pathway Enrichment Comparison analysis revealed interleukin-10 signaling and signaling by interleukins were highly expressed in TA group. Besides, extracellular matrix organization was also observed in this group. WGCNA and PPI obtained 26 core genes which were highly correlated with the clinical phenotype. We then also perform deconvolution of the bulk RNA-seq data by using the scRNA-seq dataset and noticed the high proportion of smooth muscle cells in our dataset. Additionally, immunohistochemical staining confirmed our bioinformatic analysis that TA aortic tissues express high levels of IL-1R1 and IL-1R2. Briefly, this study revealed critical roles of interleukins in TA pathogenesis, and SMCs may also participate in the reconstruction in vessel wall at late stage of TA.

Summary: To deepen the knowledge about epidemic prevalence in the goose breeding field, a triplex PCR assay was established, and 478 samples were collected from scaled goose farms in 11 provinces in China. The results of this epidemiological investigation showed that incidence rates of H9 avian influenza and goose circovirus were the highest among five infectious diseases that were evaluated. In addition, the triplex PCR assay established remarkable sensitivity, rapidity and versatility compared to other diagnostic methods. Dual infection comprised a large proportion of the co‐infections in the field, of which the combinations of H9/Tembusu, H9/goose circovirus and goose circovirus/Tembusu co‐infected cases were more common. Epidemics were more severe in winter and spring. Additionally, significant differences in the prevalence of these infectious diseases were observed in association with different age groups. In addition, phylogenetic analysis, determined by the neighbour‐joining method, was carried out to investigate the evolution of these viruses during the study period. For the most part, virus strains isolated during the study were consistent with most goose‐origin strains isolated from the Chinese mainland over the past few years. However, mutations were observed between isolated H9 avian influenza virus strains and sequences available from GenBank, which should draw much attention. [ABSTRACT FROM AUTHOR]