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Yoghurt made from milk, colostrum and their combination are one of the innovations in product development. Colostrum is rich in nutrients that can be used as a functional food ingredient. This study aimed to analyze the physicochemical properties (colour, total solids, and fatty acids) and functional properties (antioxidant activity and fatty acid profile) of yoghurt made of additional colostrum. Colostrum is rich in nutrients for functional food ingredients. A total of six treatments of yoghurt made with milk-colostrum mixture (100:0%, 80:20%, 60:40%, 40:60%, 20:80% and 0:100%) added. Each treatment was repeated four times, and data obtained were analyzed with the Analysis of Variance, followed by the Duncan test using SPSS ver 25 programs. Colostrum incorporated into yoghurt making could increase the yellow colour and the percentage of lactic acid. The research conclusion is the use of colostrum in making yoghurt can increase the yellow colour and the percentage of lactic acid. The highest antioxidant activity was observed in the production of yoghurt from the milk-colostrum mixture (20:80%). Yoghurt made of 100% colostrum could increase oleic acid but decrease palmitic acid and myristic acid.

The purpose of the study was to determine the quality of whey obtained from cow milk using different types of coagulants (microbial rennet, calf rennet, lactic acid and papaya latex). The quality of whey was evaluated with the help of chemical and microbial tests. Results revealed that maximum whey yield (765 mL/L milk) was obtained from lactic acid coagulation which was 17 mL higher than that of the microbial rennet coagulation (p=0.000). The pH was found significantly (p

Angiosarcoma of the breast is an unusual malignancy and carries a poor prognosis, with a 5-year overall survival rate ranging from 27 to 48%. Radiotherapy-induced angiosarcoma (RIAS) of the breast is very uncommon, with an estimated incidence of 1 in 1,000 cases of breasts treated with radiotherapy for breast cancer. The increase in radiotherapy usage may lead to an increased incidence of RIAS. A case presentation of a 67-year-old patient with tubular adenocarcinoma of the left breast who developed c-MYC-positive RIAS of the breast is presented. The patient was successfully treated with surgery. We presented a classic case of c-MYC RIAS. c-MYC was reported to be positive in RIAS and other types of angiosarcomas. Clinical examination and early detection of RIAS breast angiosarcoma is vital to improving outcomes in these patients.

Background: Secretory immunoglobulin A, human ß-defensin2 and calprotectin play an essential role in digestive system defense. This study aims to assess fecal SIgA, hBD-2, and calprotectin in preterm neonates (as inflammation marker) with different feeding patterns. Method: This study was cohort designed included 39 preterm neonates, which were categorized into breast milk only, formula milk only, and combination. Fecal SIgA, hBD-2, and calprotectin measured by ELISA. Results: Fecal SIga in the breast milk only group were significantly higher (p

In this study, it was aimed to reveal the structural properties and the effects of husbandry practices on milk yield in dairy farms of Ondokuz Mayıs district of Samsun. While the data were obtained by face-to-face surveys and observations in a total of 59 farms those had ≥10 head cattle between January and June 2018, environmental factors affecting daily milk yield (DMY) were separately evaluated. Important negativeneses were determined on the barn type, bedding material using, calving pens, weaning period of calves, claw care and udder cleaning in the farms. It was informed that local bazaars and cooperatives were commonly used for raw milk marketing, and high feed price was the most important problem. DMY was significantly affected by the experience of farm owner, number of the milking cows, amount of concentrate feed that given and milking type. It was concluded that farm properties and herd management methods have to be restored to elevate milk production in the investigated farms.

Due to the diversity and limitation of determination methods, published data on the fatty acid (FA) compositions of different milk samples have contributed to inaccurate comparisons. In this study, we developed a high-throughput gas chromatography-mass spectrometry method to determinate milk FA, and the proposed method had satisfactory linearity, sensitivity, accuracy, and precision. We also analyzed the FA compositions of 237 milk samples from Holstein cows, Jersey cows, buffalos, yaks, humans, goats, donkeys, and camels. Holstein, Jersey, goat, and buffalo milks contained high content of even-chain saturated FA, whereas goat milk had higher content of medium- and short-chain FA (MSCFA). Yak and camel milk are potential functional foods due to their high levels of odd- and branched-chain FA and low ratios of n-6 to n-3 polyunsaturated FA (PUFA). Human milk contained lower levels of saturated FA, MSCFA, and conjugated linoleic acid, and higher levels of monounsaturated FA and PUFA. As a special nonruminant milk, donkey milk contained low levels of monounsaturated FA and high levels of PUFA and MSCFA. Based on the FA profiles of 8 types of milk, nonruminant milk was distinct from ruminant milk, whereas camel and yak milk were different from other ruminant milks and considered as potential functional foods for balanced human diet.

International audience; The unexpected roles of the microbiota in cancer challenge explanations of carcinogenesis that focus on tumor-intrinsic properties. Most tumors contain bacteria and viruses, and the host’s proximal and distal microbiota influence both cancer incidence and therapeutic responsiveness. Continuing the history of cancer–microbe research, these findings raise a key question: to what extent is the microbiota relevant for clinical oncology? We approach this by critically evaluating three issues: how the microbiota provides a predictive biomarker of cancer growth and therapeutic responsiveness, the microbiota’s causal role(s) in cancer development, and how therapeutic manipulations of the microbiota improve patient outcomes in cancer. Clarifying the conceptual and empirical aspects of the cancer-associated microbiota can orient future research and guide its implementation in clinical oncology.

Background/purpose: Acute gastroenteritis (AGE) remains a significant health issue in children. The worldwide evolution of pediatric AGE pathogens had been recorded since the introduction of rotavirus vaccine. Ten years after the rotavirus vaccine was introduced to the private sectors in Taiwan, a nationwide study was conducted to elucidate the epidemiological changes among major AGE pathogens. Methods: From January 2014 to December 2017, children younger than 5 years old, hospitalized with AGE at 10 hospitals across Taiwan were enrolled. Stool specimens were tested for Salmonella spp., Campylobacter spp., Clostridiodes difficile, norovirus, and rotavirus by polymerase chain reaction (PCR). The epidemiological and clinical information was collected. Results: Enteric pathogen were detected in 1983 (42.2%) of 4700 subjects, with Salmonella spp. (12.5%) being the leading cause of AGE, followed by norovirus (11.2%), rotavirus (8.7%), C. difficile (4.2%), Campylobacter spp. (1.0%), and a mixture of at least 2 of 5 above-mentioned pathogens (4.6%). The case distributions varied across different regions. In eastern Taiwan, rotavirus (21/131, 16.0%) remained the most common pathogen detected. The rotavirus vaccine uptake rate is significantly lower in patients with rotavirus AGE. Besides, rotavirus AGE frequently occurred in children with foreign parent(s), Taiwanese indigenous people, and those with the household monthly income

The gastric digestion behavior of differently processed goat milks was investigated using a dynamic in vitro gastric digestion model, the human gastric simulator. Homogenization and heat treatment of goat milk resulted in gastric clots with highly fragmented structures. They also delayed the pH reduction during digestion, altered the chemical composition of the clots and the emptied digesta, promoted the release of calcium from the clots, and accelerated the hydrolysis and the emptying of milk proteins. The apparent density of the protein particles and the location of the homogenized fat globules changed during the digestion process, as shown in the emptied digesta of the homogenized goat milks. The effects of processing on the digestion behavior of goat milk were broadly similar to those previously reported for cow milk. However, the overall gastric digestion process of goat milk was more affected by homogenization than by heat treatments.

Since the 2002 Institute of Medicine report, which many cite as a landmark in first defining and calling attention to the concept of health disparities in medicine, much work has been dedicated to characterizing health disparities in medical care with the aim of eliminating them. Importantly, this report, "Unequal Treatment: Confronting Racial and Ethnic Disparities in Health Care," laid bare the differences in quality of health care that are based on race, ethnicity, and socioeconomic status. Here, the authors elaborate on these issues and discuss the role of the neuro-oncologic workforce in potentially mitigating these disparities.

Histamine is an active amine compound that occurs in various fermented foods that may cause adverse effects on the human health. Certain microorganisms are able to degrade histamine by an oxidative deamination reaction. Therefore, the present study aimed to quantify histamine‐forming and/or ‐degrading activity of the isolates derived from milk of goat and sheep herds, in Iran, by the capillary zone electrophoresis (CZE) method; and we evaluated the molecular characteristics of staphylococcal isolates. Among 243 staphylococcal isolates, 29 histamine‐degrading bacteria were identified. One of these isolates, identified as Staph. epidermidis, No. 605, exhibited the highest activity compared to others, degrading available histamine to 58.33% within 24 h. By polymerase chain reaction (PCR) analysis, the isolate, No. 605 that exhibited remarkable histamine‐degrading activity lacked the genes encoding coagulase and DNase, nor did it harbor any of the five classical enterotoxin genes. This is the first report to show that seven Staphylococcus species, including Staph. chromogenes, Staph. aureus, Staph. haemolyticus, Staph. epidermidis, Staph. pseudintermedius, Staph. agnetis, and Staph. hyicus, were able to degrade histamine, which were hitherto not known to have this capacity. Therefore, histamine‐degrading activity is a definite criterion to introduce fermenting organisms able to decrease histamine content in different food products.

The aim of the present study was to explore the effect of oropharyngeal mother’s milk administration on salivary secretory immunoglobulin A (sIgA) levels in preterm infants fed by gastric tube. Infants (n = 130) with birth weight

International audience; Edema disease is an often fatal enterotoxemia caused by specific strains of Shiga toxin-producing Escherichia coli (STEC) that affect primarily healthy, rapidly growing nursery pigs. Recently, outbreaks of edema disease have also emerged in France in wild boars. Analysis of STEC strains isolated from wild boars during 2013-2019 showed that they belonged to the serotype O139:H1 and were positive for both Stx2e and F18 fimbriae. However, in contrast to classical STEC O139:H1 strains circulating in pigs, they also possessed enterotoxin genes sta1 and stb, typical of enterotoxigenic E. coli. In addition, the strains contained a unique accessory genome composition and did not harbor antimicrobial-resistance genes, in contrast to domestic pig isolates. These data thus reveal that the emergence of edema disease in wild boars was caused by atypical hybrid of STEC and enterotoxigenic E. coli O139:H1, which so far has been restricted to the wildlife environment.Keywords

Consumers demand for food safety affects dairy industry, restraining the sector to adapt to milk quality parameters established by legislation, such as somatic cell count (SCC) and standard plate count (SPC). Good agricultural practices have positive impact over these parameters, especially Forestripping Milk (FSM), which consists of observing the first milk streams collected in a dark-bottomed mug for identification of clinical mastitis. These first milk streams have high somatic cells count and bacteria. Therefore, the aim of this study was to evaluate the characteristics of milk from FSM, such as SCC and SPC values, and its impacts on milk quality of the cooling tank through simulating contamination. Fourteen dairy farms and one experimental farm were evaluated for SCC and SPC parameters of FSM. It was observed that there was no statistically significant difference for SCC and SPC between milk samples from the cooling tank and cooling tank simulating the inclusion of milk from FSM. Statistically significant difference for somatic cell score (SCS) and SPC was observed when tanks were classified by SCC. In addition, difference in SCS was observed between morning and afternoon milking. The collection of first milk streams can contribute to decrease in SCC and SPC of milk in the cooling tank. FSM must be practiced by all dairy farmers as milking management, to assist in clinical mastitis diagnosis and improve milk quality.

Introduction The roles of Bacteroides species in alleviating inflammation and intestinal injury has been widely demonstrated, but few studies have focused on the roles of Bacteroides vulgatus. Objectives In this study, four B. vulgatus strains were selected, based on their genomic characteristics, to assess their ability to alleviate lipopolysaccharide (LPS)-induced acute intestinal injury in C57BL/6J mice. Methods Alterations in the intestinal microbiota, intestinal epithelial permeability, cytokine level, short-chain fatty acid (SCFA) concentration, and immune responses were investigated following LPS-induced acute intestinal injury in C57BL/6J mice. Results Severe histological damage and a significant change in cytokine expression was observed in the mouse colon tissues 24 h after LPS administration. Oral administration of different B. vulgatus strains showed different effects on the assessed parameters of the mice; particularly, only the administration of B. vulgatus FTJS7K1 was able to protect the architectural integrity of the intestinal epithelium. B. vulgatus FTJS7K1 also negated the LPS-induced changes in cytokine mRNA expression in the colon tissues, and in the proportion of regulatory T cells in the mesenteric lymph node. Compared with the LPS group, the B. vulgatus FTJS7K1 group showed significantly increased abundance of Lactobacillus, Akkermansia, and Bifidobacterium, and decreased abundance of Faecalibaculum. The B. vulgatus FTJS7K1 group also showed significantly increased concentration of SCFAs in fecal samples. The results of genomic analysis showed that these protective roles of B. vulgatus FTJS7K1 may be mediated through specific genes associated with defense mechanisms and metabolism (e.g., the secretion of SCFAs). Conclusions Our findings suggest that the protective role of B. vulgatus FTJS7K1 appear to be via modulation of cytokine production in the colon tissue and regulation of the structure of the gut microbiota. These results provide support for the screening of the Bacteroides genus for next-generation probiotics.

The main objective of this experiment was to evaluate the effects of two milking intervals (8 and 16 h) on milk constituents (fat, protein, lactose, dry matter, and log10 SCC) of nineteen Istrian × Awassi × East-Friesian crossbred ewes in different milk fractions (0–25, 25–50, 50–75 and 75–100%) during the course of milking and in machine stripping (MS) milk. Furthermore, we sought to determine the effect of the two milking intervals on milking characteristics (average milk flow rate, peak milk flow rate, machine-on time, total milk yield, and milk production rate) and whether each milk constituent within each milking interval is best described by a linear, quadratic, or cubic function. Average milk flow rate and milk yield per milking decreased in the 8 h milking interval compared to the 16 h milking interval (P < 0.05). Peak milk flow rate, machine-on time, and milk production rate were not different between the two milking intervals. Overall, milk fat content, dry matter content, and log10 SCC increased in the 8 h milking interval compared to the 16 h milking interval (P < 0.05). Milk protein content did not change through the main milk fractions at either milking interval. Milk lactose content did not change through the milk fractions at the 8 h milking interval, whereas it decreased in the 75–100% and stripping milk fractions at the 16 h milking interval (P < 0.05). The 0–25% and stripping milk fractions contained the highest log10 SCC compared to all other milk fractions (P < 0.05). Changes of milk fat and dry matter content throughout milking were best described by quadratic functions, whereas milk protein content, milk lactose content, and log10 SCC were best described by different functions depending on the milking interval. These results demonstrate that milking interval influenced all milk constituents in various milk fractions during the course of ewe milking. Moreover, milking characteristics such as average milk flow and total milk yield, and the appropriate mathematical function to characterize milk constituents throughout a milking, were affected by milking interval.

Introduction Exclusive human milk feeding improves outcome in preterm infants however implementation is inadequate. Methods This randomized controlled trial was conducted in a tertiary care hospital (6 months) to assess the effect of antenatal and postnatal individualized counseling on exclusive human milk feeding in preterm infants. In intervention counseling regarding benefits of exclusive human-milk feeding and demonstration of expression of milk. Outcomes were proportion of infants on exclusive human-milk feeding,type of milk and method of feeding during hospital stay and at discharge. Results At discharge, (97%) were on exclusive human-milk in intervention group as compared to (6%) in control group (p Conclusion Individualized counseling improves exclusive human-milk feeding in preterm infants.

Owing to the lack of research on structure-activity relationship and interaction mechanism between unsaturated polyester resins (UPR) and flame retardants, it has been a big challenge to prepare high-efficiency flame retardants for UPR in industry. In this research, to explore structural rules of high-efficiency flame retardants, several polymeric flame retardants were synthesized with varied main-chain, side-chain, phosphorus valence states and contents of flame retardant elements. The thermal stabilities of flame retardants and UPR composites were firstly assessed. It has been found the interaction existed between flame retardants and UPR, through transesterification reaction and β scission pathway in polyester and polystyrene chains. With only 15 wt% of PCH3-S, UPR composites can reach V0 rating in UL-94. The PHRR and THR values can be maximumly decreased by 71.66 % and 77.67 %, with 20 wt% of PB-S. It has been found flame retardants with sulfone group and + 3 valence state of phosphorus in molecular backbone can release SO2 and phosphorus containing compounds in gaseous phase, which diluted fuel fragments and catalyzed H⋅ and HO⋅ radical removal. The mechanism for improved flame retardancy of UPR composites with various polymeric flame retardants were discussed in detail. Some general rules for highly efficient flame retardant UPR can be summarized: First, gaseous phase flame retardant mechanism plays the major role in improvement of flame retardant performance of UPR composites; Second, the combination of + 3 valence state of phosphorus structures, higher phosphorus contents and sulfone groups effectively improves the flame retardant efficiency of flame retardants.

Gall-inducing organisms change the development of their host plant organs, resulting in ontogenetic patterns not observed in the non-galled plants. Distinct taxa induce galls on Schinus spp., manipulating meristematic patterns in the host plant in distinct ways. Here we report ontogenetic novelties induced in the lateral buds of S. engleri by Eucecidoses minutanus, a Cecidosidae, whose galls have been poorly understood.The anatomy, histochemistry, and histometry of galls in distinct phases of development, non-galled buds, and stems of Schinus engleri were analyzed in parallel with the instars of E. minutanus to detail the morphogenetic changes in the host with each larval stage.Ontogenetic phases of the galls were intricately associated with larval development. First and second-instar larvae induced pericycle and pith cells to dedifferentiate into the gall inner meristem, where hyperplasia and cell hypertrophy characterized the growth and development phase of the gall. The innermost layers were lipid-rich nutritive cells that lined the larval chamber. Additional vascular bundle rows were produced in young galls. Third and fourth instar-larvae were associated with the gall maturation phase: centripetal lignification of the outer parenchyma cell layers, epidermal stratification, and activation of a cambium-like meristem (CLM). The CLM activity resulted in new layers of nutritive cells that differentiated inward as the first layers of nutritive cells were consumed by E. minutanus larvae, and, also, in more parenchyma cell layers that formed outward. All tissues between the innermost layer of nutritive tissue that surround the gall chamber and the outermost layer of the dermal system that externally covers the gall form the gall wall, and increased in thickness until the end of gall maturation.E. minutanus induces a structurally complex globoid stem gall, modifying all host plant tissues and stimulating a novel meristematic pattern in S. engleri. The gall developmental stages are each related to specific gall-inducing instars, as gall development progresses according to the development of E. minutanus.

Humor has been characterized as an index of children’s language development and cognitive performance. While research on this area has been abound cross linguistically, specific inquiry on children’s humor development in the Indonesian context remains scant. Occupying this lacuna, this research sought to examine kinds of humor acquired by the children in early childhood by invoking McGhee’s humor developmental stages. Embracing a descriptive qualitative method, the study involved eleven Indonesian-speaking children in Yogyakarta aged around 1 to 5 years old, where the data were assembled primarily through direct naturalistic observations. The children’s spontaneous humor production was recorded as they were interacting with their peers and/or their parents in their homes and neighborhoods. The findings revealed that the children could perceive and produce nonverbal and verbal humor, confirming existing literature. As they grow, certain patterns of humor also emerge, indicative of their cognitive and social development. More research is necessary to better understand how children of different linguistic and cultural backgrounds understand, respond to or initiate a humorous situation.

Background: Elizabethkingia spp. are Gram-negative, non-spore-forming bacteria that used to be rarely encountered but now they are of growing clinical significance. Furthermore, the diagnostic challenges to identification impede the complete elucidation of their epidemiology and association with human diseases. Case report: A 64-year-old man with liver cirrhosis due to alcohol abuse presented with fatigue, abdominal swelling, and bilateral scrotal edema. A peritoneal fluid sample was cultured and subject to a number of identification methods – API 20 NE (bioMerieux), automated Vitek-2 Compact (bioMerieux), MALDI-TOF MS (Vitek-MS, bioMerieux), and 16S rRNA gene sequencing. A final diagnosis of bacterascites was made on the basis of clinical, laboratory, and microbiological findings. Conclusion: To the best of our knowledge this is the first report of Elizabethkingia miricola being cultured from a peritoneal fluid sample in an immune-compromised host thus pointing to the emerging pathogenic role of the bacterium in patients with liver cirrhosis and ascites.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can be detected in the stool samples of patients with coronavirus disease 2019 (COVID-19), and this virus can be transmitted via the oral-fecal route. However, there are only few reports on the viral load in the stool samples. In this pilot study, we aimed to evaluate the clinical characteristics and viral load of SARS-CoV-2 in the stool samples of 13 patients with confirmed COVID-19 using pepper mild mottle virus as a control, which has been proposed as a potential marker of human feces contamination in the environmental water bodies. SARS-CoV-2 RNA was detected in the stool samples of four patients (31%), and among them, three exhibited symptoms of diarrhea. One patient who suffered from long-term diarrhea (22 days) exhibited highest level of viral RNA in the stool sample (8.28 log10 copies/g). However, we could not harvest SARS-CoV-2 from the stool sample of any patient, even after culturing with VeroE6/TMPRESS2 cells for four weeks. Our results suggest that SARS-CoV-2 RNA can be detected in the stool samples of patients with COVID-19 suffering from diarrhea. However, further studies elucidating the relationship between SARS-CoV-2 viral load in the stool samples and symptoms of diarrhea in large cohorts and upon adjusting other causative factors and virus infectivity are still warranted.

Enteritis cystica profunda (ECP), a rare and benign condition, is defined as the displacement of the glandular epithelium into the submucosa and more profound layers of the small intestinal wall leading to the formation of mucin-filled cystic spaces. ECP frequently occurs in the ileum or jejunum and is associated with diseases such as Crohn disease and Peutz-Jeghers syndrome. ECP also develops in the absence of known pathology. ECP in the duodenum is very rare and mostly occurs without associated conditions. In this report, we present a rare case of ECP without an associated disease, in the second portion of the duodenum distal to the ampulla of Vater and coexisting with lipoma within the polypoid lesion.

Objective In patients with Tourette syndrome and other primary tic disorders (PTDs), tics are typically preceded by premonitory urges (PUs). To date, only a few studies have investigated the location and frequency of PUs, and contrary to clinical experience, the results suggest that PUs are not located in the same anatomic region as the tics. This study aimed to further explore PU location and frequency in detail, differentiating the kind and complexity of the corresponding tics, in a large sample of patients with PTD.Methods A total of 291 adult (≥ 18 years) patients with a confirmed diagnosis of chronic PTD were included. The study was conducted online, assement included tics and the general characterization of PUs and a sophisticated body drawing for locating PUs.Results We found that PUs were located in the same body area as, or in direct proximity to, the corresponding tic. Most frequently, PUs were located in the face and at the head (62.1%). Compared with simple tics, complex (motor and vocal) tics were more often preceded by a PU; but there was no difference in PU frequency observed between motor tics and vocal tics. PUs were more often experienced at the front than at the back of the body (73% vs. 27%), while there was no difference between the right and left sides (41.6% vs. 41.3%).Conclusion The strong association between PU and tic location further supports the hypothesis that PUs represent the core of PTD. Accordingly, future therapies should focus on treating PUs to achieve greater tic reduction.

Purpose is to study the effect of milk production technology in the SPK “Glinskiy” with the use of robotic milking machines Lely Astronaut, DeLaval and linear milking machine ADM-8A, on the efficiency of milk production. Methods. An analysis of the efficiency of milk production with various technologies of keeping and milking cows was carried out on the basis of materials for accounting for milk productivity obtained for 2016–2019, and also the marketability of milk, the cost per unit of milk and the profitability of milk production during robotic milking and milking in a milk pipeline were determined. Scientific novelty. The analysis of the economic efficiency of milk production in the conditions of one farm is carried out, the differences in the level of milk productivity and milk quality are determined. Calculated: the cost price of 1 kg of milk obtained using the milking machine ADM-8 and automated milking machines Lely and DeLaval, profitability. Results. A large number of factors, both genetic and paratypical, affect the milk production of cows. The main paratypical factor is milk production technology, which includes keeping, feeding and milking livestock. Nowadays, the widespread technology is tethering cows with milking in a long milk pipe in stalls, this technology is considered traditional. She is being replaced by a more productive one – keeping a milking herd without a tether in groups with milking on automatic milking machines (robotic milking). Feeding with these two technologies is organized according to different principles. Our study revealed the influence of milking technology on the efficiency of milk production. As a result of the study, differences in the level of milk productivity and milk quality were determined. Our study revealed a positive effect of milking technology with the help of robotic installations on the efficiency of milk production: milk yield for each lactation increases by 7–28 %, which is confirmed by the lactation constancy index, the content of somatic cells in milk decreases to 2.4 × 105 cells per 1 cm3 providing an increase in the marketability of milk to 97.4%, in terms of physical and chemical indicators, milk of cows, with different methods of keeping, was within the normal range and average values for the herd.

The aims of this study were to determine the characteristics and quality of fresh semen from Sumba Ongole (SO) bulls which were diluted with tris dilution supplemented with skim milk. The study used a completely randomized design consisting of 4 treatments and 5 replications. The treatments were P0 = 100 % Tris, P1 = 88 % Tris + 12 % Susu skim, P2 = 86 % Tris + 14 % Susu skim, dan P3 = 84 % Tris + 16 % Susu skim. The parameters observed were the colour, smell, consistency, pH, volume, motility, viability, and abnormalities of semen. The results showed that the characteristics of the semen were normal, the color of the semen was creamy white, the semen volume was 4.6 ml, the consistency was moderate, pH 6.3, the smell was typical of SO cattle, the motility was 80%, the mass movement was +++, the concentration was 758 million/ ml, viability is 74.24% and abnormality is 16.12%. The characteristics and quality of cement in the four treatments showed no significant difference (P>0.01). The motility and viability of spermatozoa in each treatment were able to survive up to day 3. The Tris diluent with the addition of skim milk was not able to maintain the characteristics and quality of the semen.

Irritable bowel syndrome (IBS) is a common clinical label for medically unexplained gastrointestinal symptoms, recently described as a disturbance of the microbiota-gut-brain axis. Despite decades of research, the pathophysiology of this highly heterogeneous disorder remains elusive. However, a dramatic change in the understanding of the underlying pathophysiological mechanisms surfaced when the importance of gut microbiota protruded the scientific picture. Are we getting any closer to understanding IBS' etiology, or are we drowning in unspecific, conflicting data because we possess limited tools to unravel the cluster of secrets our gut microbiota is concealing? In this comprehensive review we are discussing some of the major important features of IBS and their interaction with gut microbiota, clinical microbiota-altering treatment such as the low FODMAP diet and fecal microbiota transplantation, neuroimaging and methods in microbiota analyses, and current and future challenges with big data analysis in IBS., Supported by the Spanish Ministry of Science and Innovation (MICINN, Spain), No. AGL2017-88801-P (to Sanz Y); the Miguel Server grant from the Spanish "Carlos III" Health Institute (ISCIII), No. CP19/00132 (to Benitez-Paez A); The Norwegian Research Council (Funding Post-doc Position for Bharath Halandur Nagaraja), No. FRIMEDBIO276010; and Helse Vest’s Research Funding, No. HV912243; and ERC H2020-MSCA-IF-2019, No. 895219 (to Haleem N).

Milk is a highly nutritious food and it is important to be free of any pathogenic microbes that could be transmitted to humans and affect public health. A total of 145 milk samples were collected from humans and different animal species (cow, buffalo, ewe, goat, camel, mare, and donkey) and underwent physical examination (color, odor, and taste), chemical analysis for its components (water, total salt, fat, protein, lactose, and ash), and finally microbiological (bacteriological and mycological) examinations. Standard plate count, preliminary incubation count, lab pasteurized count, coliform, Escherichia coli, Staphylococcus species, Salmonella species, yeast, and mold counts were measured. PCR test was performed to differentiate milk from different sources (animals and humans) by producing a specific band for each milk type. The results of the physical examination of different kinds of milk showed different grades of white color with the characteristic odor and taste of each milk type. Chemical examination revealed that the highest water content was in donkey milk and the lowest was in buffalo milk while total solids indicated the highest content in sheep milk and the lowest in donkey milk. The microbiological analysis presented that the standard plate count results were the highest in the milk obtained from sheep and camel, while donkey milk was the least in this regard. Yeast counts were the highest in buffalo milk but cow milk was the highest in mold counts. PCR results of milk types using species-specific primers and DNA template extracted from milk somatic cells revealed a specific band for each milk type as 157, 195, 225, 242, 274, and 711 base pair (bp) for goat, human, sheep, buffalo, cattle milk, and camel milk, respectively. It was concluded that more restrictions must be applied to decrease milk contamination as high microbial counts detected in the present study can affect milk quality, public health, and the dairy industry. PCR used in the current work for milk discrimination used milk somatic cells specifically mitochondrial cytochrome b gene which exhibited high specificity in the PCR reactions and this could be served as a cheap and simple method, compared to other types of PCR.

Background: Tuberculosis is a chronic infectious disease caused by Mycobacterium tuberculosis and still it is a common disease in developing countries. A variety of hematological parameters are studied in tuberculous patients such as decreased Hb(anemia), increased WBC count, increased ESR, and thrombocytopenia. Nowadays TB is a curable and preventable disease. The present study was undertaken to study the ESR values in TB patients and compare their values with ZiehlNelson staining. Method: The present study was carried out on 328 patients who had sputum smear-positive for AFB. based on the recommendation of the RNTCP grading system of AFB in Sputum ZiehlNelson staining, the cases of PTB group were further categorized into four subgroups AFB– 3+, AFB – 2+, AFB –1+ and scanty. The ESR was performed by a rapidly modified Westergren method on EDTA whole blood sample. Results: out of the 324 cases of tuberculosis collected from our hospital, male 252, female 72 from 14-80 years of age. ESR was elevated in patients with an increased grading of sputum positive (3+). The distribution of the mean value of, ESR in different grades of ZN staining of sputum in AFB positive patients was scanty(35.5), 1+(27.5), 2+(30.2),3+(42.4). respectively. Conclusion: Our study concluded that active TB is associated mostly with very high ESR values (≥ 100 mm/h) and with markedly raised values in all the grades of sputum AFB positive patients.

Data on the epidemiology of Entamoeba infections in Eastern part of Kenya is scanty. Diagnostic tests in use have limited capacity to differentiate common infecting species. The performance of Polymerase Chain Reaction (PCR) to differentiate between pathogenic Entamoeba histolytica and two nonpathogenic species E. dispar and E. moshkovskii is largely undetermined. Therefore, this study sought to determine the prevalence and associated factors for Entamoeba infections and evaluate the performance of PCR to differentiate Entamoeba complex species among diarrheal patient in Eastern region of Kenya. Stool samples were obtained from 400 patients attending Meru Teaching and Referral Hospital (MTRH) presenting with diarrhea. Samples were processed by direct wet mount using normal saline and iodine stain for microscopic examination. Entamoeba species differentiation was done using PCR targeting the 16S rRNA gene. A total of 33 (8.3%) samples had Entamoeba cysts/trophozoites by microscopy while 29 (7.3%) were identified as E. histolytica by PCR. Entamoeba infections was most common among adults 23 (5.8%) and in females 20(5%). The sensitivity of microscopy was 29/29 (100%; 95% CI 88.1% - 100%) with a specificity 367/371 (98.9%; 95% CI 97.3 % - 99.7%). In multivariate analysis, factors that independently influenced Entamoeba infection included sources of drinking water, use of toilet with water, regular use of soap or sanitizer, having diarrhea that persists for two weeks and stool consistency. Entamoeba infection was found to be responsible for most diarrhea condition especially among children. Patients hygienic and sanitation characteristics contributes significantly to Entamoeba infection. The performance of microscopy to detect Entamoeba infection is comparable to those of PCR except for the lack of species differentiation. Molecular species differentiation will improve disease diagnosis, control and management. Continuous monitoring of patient presenting with diarrhea for Entamoeba infection would improve treatment outcomes.

Fms-like tyrosine kinase 3 (FLT3) has been verified as a therapeutic target for acute myeloid leukaemia (AML). In this study, we report a series of 2-(1H-indazol-6-yl)-1H-benzo[d]imidazol-5-yl benzamide and phenyl urea derivatives as potent FLT3 inhibitors based on the structural optimisation of previous FLT3 inhibitors. Derivatives were synthesised as benzamide 8a���k, 8n���z, and phenyl urea 8l���m, with various substituents. The most potent inhibitor, 8r, demonstrated strong inhibitory activity against FLT3 and FLT3 mutants with a nanomolar IC50 and high selectivity profiles over 42 protein kinases. In addition, these type II FLT3 inhibitors were more potent against FLT3 mutants correlated with drug resistance. Overall, we provide a theoretical basis for the structural optimisation of novel benzimidazole analogues to develop strong inhibitors against FLT3 mutants for AML therapeutics.

At the present stage of development of dairy cattle breeding, the choice of machine milking technology is a priority task in the operation of any dairy complex, and the study of its impact on cows productivity when using modern milking equipment is timely and relevant. The purpose of the work was to study the technology of robotic milking of cows and evaluate the effectiveness of its application under the conditions of the dairy complex for industrial milk production. It has been found as a result of the carried out research that the use of a robotic milking machine is possible with the free stall housing of cows with their free access to the milking parlor with the installation of Lely Astronaut milking robots. The farm uses fl ow-sections technology: the first section is the milk production, the second section is for calving and the third section is for dry cows. The installation provides the individual approach to milking the animal when assessing the health of the udder, namely, separate milking of the udder quarters, which allows, when mastitis is detected, to dump milk from the diseased udder quarter into a separate container. Assessment of udder health is carried out by changing the physical properties of milk in case of disease, i.e. electrical conductivity. At the same time, the system evaluates the quality of milk and collects data on each animal individually and on the quarters of the udder. Each of the two robots installed on the farm is capable of milking 65 cows. 123 cows located in a room where the voluntary milking system is installed serve only 2 people per shift. Robots allow us to save on milking facilities, reduce staff maintenance costs and increase the salaries of service personnel. The payback of the equipment – a robotic installation for milking cows Lely Astronaut will be with the productivity of animals ranging from 6000 to 6500 kg on average per cow for about 3 years.

Cystic fibrosis is a disease caused by mutations in a gene encoding CFTR-protein (Cystic Fibrosis Transmembrane conductance Regulator), located in the apical membrane of epithelial cells of the respiratory tract, intestines and pancreas. Defensins serve as important components of the innate human immune system, they play a key role in providing the first line of defense of a macroorganism against infection; they have high antimicrobial, antiviral, cytotoxic activity.Objective. To determine the values of fecal β-defensin-2 in children with cystic fibrosis and to reveal the dependence of its level on the exocrine function of the pancreas and the severity of the patient’s condition.Characteristics of children and research methods. The study included 57 children with cystic fibrosis, the average age was 20.93 ± 2.9 months. Cystic fibrosis was diagnosed on the basis of an increase in immunoreactive trypsin, sweat chlorides by Cook’s method (>60 meq / l). To assess the exocrine function of the pancreas the scientists determined the activity of fecal elastase. They evaluated the levels of fecal β-defensin-2 and calprotectin using a quantitative enzyme immunoassay.Results. The levels of fecal β-defensin-2 were increased (108.2 ± 11.3 ng / ml) in all children under examination. The researchers found no correlation between the levels of fecal β-defensin-2 and fecal elastase. The level of fecal calprotectin was significantly higher in the group of children with cystic fibrosis as compared to the control group. There was a significant correlation between the levels of fecal calprotectin and fecal β-defensin-2 (r=0.57; p Conclusion. Children with cystic fibrosis demonstrated a significant increase in the concentration of β-defensin-2 as compared to the control group, which confirms the activation of the innate immune system of the intestinal mucosa. The researchers traced the relationship between high levels of fecal β-defensin-2 and the severity of the disease. The levels of fecal β-defensin-2 directly correlated with the concentration of fecal calprotectin and there was no correlation between the severity of pancreatic insufficiency and the concentration of fecal β-defensin-2.

Fecal microbiota transplantation (FMT) has been used in a wide variety of diseases. Many researchers hypothesize that the dysbiosis of intestinal microbiota plays an important role in the development of gut-derived infections; thus, FMT is a potential therapeutic target against multiple organ dysfunction syndrome (MODS). A 106-year-old male patient was initially diagnosed with cerebral infarction and pulmonary infection. During the course of hospitalization, the patient developed MODS. The patient received a single nasogastric infusion of sterile-filtered, pathogen-free feces from a healthy donor. Fecal samples were collected every two days post-infusion to monitor changes in the microbiota composition in response to treatment. After FMT, MODS and severe diarrhea were alleviated; the patient’s fecal microbiome diversity resembled that of the healthy donor’s fecal microbiome; moreover, his clinical symptoms improved remarkably with the changes in fecal microbiome. Additionally, no observable side effects were noted during FMT treatment. These findings warrant further investigation of FMT as a putative new therapy for treating microbiota-related diseases, such as MODS.

he aim of this study, conducted under identical environmental conditions and the stanchion barn housing system, was to compare milking performance traits and somatic cell count in milk of daughters sired by Holstein-Friesian Black-and-White bulls from various origin groups. Cows were divided into three groups depending on the region of sire's origin, i.e. Polish bulls, bulls from other European countries (the Czech Republic, France and Germany) and bulls from the USA. Analyses were conducted applying the following experimental conditions: the age group of cows (primiparous vs. multiparous), stage of lactation (≤ 40 days, from 41 to 100 days, from 101 to 200 days and > 200 days) and season of the year (spring, summer, autumn and winter). Results of this study indicate that at the stanchion barn housing system of dairy cows in order to increase milk yields the use of semen from bulls of imported Holstein-Friesian Black-and-White bulls seems to be beneficial. However, daughters of US bulls may be prone to elevated somatic cell counts in milk. In turn, cows sired by Polish bulls can be characterised by favourable milk solids contents.

A comparative study of bacterial qualities of raw milk of cow and sheep was conducted at Livestock production and management (unit), Department of NRM, faculty of agriculture, MGCGV Chitrakoot–Satna (M.P.) during January to February 2021. All sanitary precaution were followed to produce clean milk. The sample of the raw milk of three animal each were replicated ten time and tested to deyermine the standard plate count (SPC) (10t), lactic acid bacteria count (LABC) ( 10³), lipolytic bacteria count (LBC) (10²) proteolytic bacteria count (PBC) (10²) and coliform count (CC) in the raw milk. The data obtained for the a foresaid tests were subjected to statitical analysis. The result of the statical analysis showed that the differences in mean values of SPC 10t,LABC/10³,LBC/10² and PBC,10². In view of the finding and result presented above, results that the milk of all the animals was of superior quality, due to low bacteria count and absence of coliform. The bacteria quality of milk of cow was found superior than sheep milk due to minimum bacteria count of SPC, LABC, LBC and PBC and absence of coliform.

The milk production chain has relevance for the Brazilian economy, generating jobs and income. In addition, milk production, because of family-based farms, has an important social function. However, milk production is spatially heterogeneous in Brazil, especially due to the different technological patterns of production. In this context, the objective of this study was to verify the spatio-temporal distribution and dynamics of milk production in Brazil. For this purpose, milk production in Brazil in 2000 and 2016 was analyzed. The Brazilian microregions that specialize in milk production were identified using location quotient (LQ). An exploratory analysis of spatial data and Moran’s I were used to measure spatial autocorrelation among regions. Finally, principal component analysis (PCA) was used to assess the grouping relationships of variables as a function of the regions that specialize in milk production. Between 2000 and 2016, there was a decrease in the number of microregions that specialize in milk production. Thus, in 2016, approximately 20% of the microregions and over 22% of Brazilian municipalities specialized in milk production. The microregions and municipalities that specialize in milk production were concentrated mainly in the states of Minas Gerais and Goiás and in the Southern region of Brazil. There was an increase in milk productivity in all regions of the country, especially in those regions where production was concentrated. The formation of high-high clusters was found in the most productive regions of the country, i.e., in the South and Southeast, where the effects of technological spillover were observed, and the formation of low-low clusters was observed in the less productive regions, i.e., in the North and Northeast. Two main components were formed. The first component aggregated variables related to milk production in volume, and the second component aggregated variables inherent to productivity. It was possible to verify the recent growth in milk production and productivity in the country as well as to demonstrate the heterogeneity in production. Although there was a decrease in the number of microregions and municipalities that specialize in milk production, there was a concentration and increase in milk production and productivity in Brazil. A cadeia produtiva do leite possui relevância para a economia brasileira, gerando empregos e renda. Além disso, a produção de leite, em razão da produção familiar, exerce importante função social. No entanto, a produção de leite é espacialmente heterogênea no país, sobretudo pelos diferentes padrões tecnológicos de produção. Nesse contexto, o objetivo deste estudo foi verificar a distribuição e a dinâmica espaço-temporal da produção de leite no Brasil. Para isso, foram utilizados, acerca da produção de leite no país nos anos de 2000 e 2016. Por meio do Quociente Locacional (QL), foram identificadas as microrregiões brasileiras especializadas na produção de leite. Foi realizada Análise Exploratória de Dados Espaciais (AEDE) e do I de Moran, para medir a autocorrelação espacial entre as regiões. Por fim, foi empregada a Análise de Componentes Principais (ACP), para verificar as relações de agrupamento das variáveis, em função das regiões especializadas na produção de leite. Foi identificada queda no número de microrregiões especializadas na produção de leite entre 2000 e 2016, Assim, em 2016, cerca de 20% das microrregiões e pouco mais de 22% dos municípios do Brasil eram especializados na produção de leite. As microrregiões e municípios especializados na produção de leite estão concentrados, principalmente, nos estados de Minas Gerais e Goiás e na região Sul do Brasil. Houve aumento da produtividade de leite em todas as regiões do país, principalmente naquelas onde há concentração na produção. Constatou-se a formação de clusters Alto-Alto nas regiões mais produtivas do país, ou seja, a Sul e a Sudeste, onde se observa os efeitos do transbordamento tecnológico, bem como a formação de clusters baixo-baixo nas regiões menos produtivas, a Norte e a Nordeste. Foram formados dois componentes principais. O primeiro componente agregou variáveis relacionadas com a produção de leite em volume, enquanto o segundo componente relacionou variáveis inerentes a produtividade. Foi possível verificar o crescimento recente da produção e da produtividade de leite no país, bem como evidenciar a heterogeneidade na produção. Verificou-se que embora tenha ocorrido queda no número de microrregiões e municípios especializados na produção de leite, houve concentração e aumento na produção e produtividade de leite no Brasil.

Summary Temporary plugging and diverting fracturing of the horizontal well is the primary option to promote production for tight reservoirs. Successful entry of diverters into the perforation is the basis and prerequisite for effective plugging. However, the transport behavior of the diverter during multicluster fracturing remains unclear. In this paper, we build a large-scale diverter transport experimental system, capable of conducting experiments with large flow rates and high pressures. The concerned factors include the injection rate, perforation flow ratio (PFRO), fluid viscosity, and perforation angle. The results show that the diverter transport effect is significantly different because of different flow distribution among perforations. Also, the diverter can enter the perforation only when the flow rate of the perforation reaches a certain value. In addition, the minimum critical PFRO has an “oblique L-shaped” relationship with the injection rate. Although it is difficult for the diverter to enter the perforation on the high side of the horizontal wellbore, increasing the viscosity of the carrying fluid or using a multidensity mixed diverter can effectively solve this problem. Furthermore, the field case shows that the experimentally obtained diverter transport pattern can be applied to the field to predict the location of the diverter and improve the temporary plugging effect. The findings of this work lay a theoretical foundation for subsequent temporary plugging and diverting fracturing control.

Since coliform bacteria are common both in the intestine and in nature (soil, plant, etc.), they are considered as a sanitation indicator in the food industry. It is known that the majority of bacteria defined as fecal coliform in the coliform group are Escherichia coli. The presence of E. coli or fecal coliform bacteria in any sample is an indication that the necessary hygienic measures are not taken during production, storage, and sale. That means the sample is directly or indirectly contaminated with faeces, and/or other intestinal pathogens may also exist. In this study, raw milk, freshly squeezed fruit juices, unpackaged ice cream, shaved ice, and ice-cold samples were purchased from the famous touristic destination Fethiye and analyzed for coliform bacteria. For this purpose, the samples were purchased from local marketplaces, buffets, cafes, patisseries, restaurants, and roadsides at Fethiye. In total 60 samples were analyzed using Violet Red Bile (VRB) Agar. The results of coliform bacteria ranged

The primary purpose of this study is to reveal the function of humorous discourse identified in the Mind Your Language situation comedy series. The researcher adopted a descriptive qualitative approach to complete this humor research. The data were obtained from the script of that sitcom. The function of humorous discourse found in that sitcom was then classified based on Hay's framework, which divides the function of humor into three main functions: the function of solidarity, the function of power, and the function of psychology. The findings demonstrate that teasing and satire humor in Mind Your Language sitcom are utilized to express solidarity. Meanwhile, humorous discourses that trigger conflict, harm, threaten others or show disagreement are created to express the function of power. Furthermore, humorous discourse to gain an advantage, protect oneself, entertain, or boast is used to express the function of psychology. The context of the conversation given is highly significant in identifying the function of humor. The same humorous discourse provided in different contexts potentially implicates different functions of humor which indicates that determining the function of humor is heavily grounded by the context.

BACKGROUND Most very premature newborns (< 32 weeks of gestation) receive parenteral nutrition (PN) that is inherently contaminated with peroxides. Oxidative stress induced by PN is associated with bronchopulmonary dysplasia, a main pathological complication in these babies who have weak antioxidant capacity to detoxify peroxides because of their glutathione deficiency. In animals, glutathione supplementation of PN prevented oxidative stress and alveolar loss (the main characteristic of bronchopulmonary dysplasia). Of its two forms - disulfide (GSSG) and free thiol (GSH) - GSSG was used because of its better stability in PN. However, a 30% loss of GSSG in PN is observed. The potentially high therapeutic benefits of GSSG supplementation on the health of very premature babies makes the study of its stability highly important. MATERIALS AND METHODS GSSG was incubated in combination with the following components of PN: dextrose, multivitamins, Primene, Travasol, as well as with cysteine, cystine and peroxides for 24h. Total glutathione in these solutions was measured 0-24h after the addition of GSSG. RESULTS The combination of cysteine and multivitamins caused the maximum loss of glutathione. The stability of GSSG was not affected by multivitamins. The cysteine was responsible for about 20% of the loss of GSSG, in presence of multivitamins the loss reached more than 70%. Removing the cysteine prevented the degradation of glutathione. CONCLUSION GSSG reacts with cysteine to form cysteine-glutathione disulfide, another suitable glutathione substrate for preterm neonates. The study confirms that GSSG added to PN can potentially provide a precursor to de novo synthesis of glutathione in vivo. This article is protected by copyright. All rights reserved.

To study the effect of fresh camel milk, fermented camel milk and colostrums on blood glucose levels 30 Alloxan-induced diabetic rabbits were taken. Diabetes was induced in rabbits using Alloxan, the diabetic rabbits were then treated with fresh camel milk, fermented camel milk and colostrums as well as insulin for 60 days. The results demonstrated that the body weight of rabbits treated with camel milk and its derivatives found to be kept within the accepted body weights range of 1.8- 2.5 kg. The lowest serum glucose levels were recorded by Group 1 (control), Group 3 (diabetic-treated with colostrums), Group 4 (diabetic-treated with milk), Group 5 (diabetic-treated with gars) and Group 6 (diabetic-treated with Insulin16 mg/kg body weight daily for 8 weeks) and the levels found to be within the normal level range of 75- 140 mg/dl, the highest level of serum glucose level was recorded by Group 2 (diabetic-non supplemented) and it was higher than the upper limit of the normal range.

Introduction: The gut microbiota is involved in host physiology and health. Reciprocal microbiota-drug interactions are increasingly recognized as underlying some individual differences in therapy response and adverse events. Cancer pharmacotherapies are characterized by a high degree of interpatient variability in efficacy and side effect profile and recently, the microbiota has emerged as a factor that may underlie these differences. Areas covered: The effects of cancer pharmacotherapy on microbiota composition and function are reviewed with consideration of the relationship between baseline microbiota composition, microbiota modification, antibiotics exposure and cancer therapy efficacy. We assess the evidence implicating the microbiota in cancer therapy-related adverse events including impaired gut function, cognition and pain perception. Finally, potential mechanisms underlying microbiota-cancer drug interactions are described, including direct microbial metabolism, and microbial modulation of liver metabolism and immune function. This review focused on preclinical and clinical studies conducted in the last 5 years. Expert opinion: Preclinical and clinical research supports a role for baseline microbiota in cancer therapy efficacy, with emerging evidence that the microbiota modification may assist in side effect management. Future efforts should focus on exploiting this knowledge towards the development of microbiota-targeted therapies. Finally, a focus on specific drug-microbiota-cancer interactions is warranted.

Whey containing more than 50% of milk solids can be attributed to an insufficiently demanded raw material of the dairy industry. The restriction of its usage for food purposes is the high residual antigenicity of whey proteins. This work aimed to justify the possibility of using milk-containing products with whey protein hydrolysate, characterized by reduced residual antigenicity, in healthy and dietary diets. Experimental work was carried out at the All-Russian Scientific Research Institute of Dairy Industry, the Animal-Derived Food Technology department and the Core Facilities Centre "Control and Management of Energy-efficient Projects" at the FSBEI HE VSUET. Compositions were developed for a sports nutrition drink made from whey protein hydrolysate with the addition of fructose, and a milk-containing drink with the replacement of 30% skimmed milk necessary for standardization with whey protein hydrolysate. The chemical composition, physical, chemical and microbiological properties of the finished products were established. The reduction of the true protein content was proven to 1.33% for a sports nutrition drink and to 1.69% for a milk-containing drink. Peptides and proteins in the finished products have a molecular weight of less than 10 kDa, which indicates a decrease in their potential antigenicity. The developed beverages are characterized by a high content of biologically active peptides, macro- and microelements, as well as vitamins. Their quality and safety indicators meet the requirements of the TR CU 033/2013. These products can be used as part of dietary diets for people who are allergic to cow's milk proteins. The organization of their production will contribute to import substitution in the segment of low-allergenic dairy products.

Grape seed proanthocyanidin extract (GSPE) that prevents and alleviates the degenerative changes associated with aging has been receiving extensive attention. In our present work, the ageing model was induced by injection of 500 mg kg

The human microbiome has been the subject of intense research over the past few decades, in particular as a promising area for new clinical interventions. The microbiota colonizing the different body surfaces are of benefit for multiple physiological and metabolic processes of the human host and increasing evidence suggests an association between disturbances in the composition and functionality of the microbiota and several pathological conditions. This has provided a rationale for beneficial modulation of the microbiome. One approach being explored for modulating the microbiota in diseased individuals is transferring microbiota or microbiota constituents from healthy donors via microbiome transplantation. The great success of fecal microbiome transplantation for the treatment of Clostridioides difficile infections has encouraged the application of this procedure for the treatment of other diseases such as vaginal disorders via transplantation of vaginal microbiota, or of skin pathologies via the transplantation of skin microbiota. Microbiome modulation could even become a novel strategy for improving the efficacy of cancer therapies. This review discusses the principle, advantages and limitations of microbiome transplantation as well as different clinical contexts where microbiome transplantation has been applied.

Background Airway surgery presents a unique environment for operating room fire to occur. This study aims to explore the factors of combustion when using KTP laser with high flow oxygen in an ex-vivo model. MethodsThe variables tested were varying tissue type, tissue condition, oxygen concentration, laser setting, and smoke evacuation in a stainless-steel model. Outcome measures were time of lasing to the first spark and/or flame. A multivariate Cox proportional hazard model was used to determine the risk of spark and flame across the different risk factors. ResultsFor every 10% increase in oxygen concentration above 60% the risk of flame increased by a factor of 2.3. Continuous laser setting at 2.6W increased the risk by a factor of 72.8. The risk of lasing adipose tissue is 7.3 times higher than that of muscle. Charred tissue increases the risk of flame by a factor of 92.8. Flame occurred without a preceding spark 93.6% of the time. Conclusions Using KTP laser in the pulsed mode with low wattages, minimising lasing time, reducing the oxygen concentration and avoiding lasing adipose or charred tissue produce a relatively low estimated risk of spark or flame.

The Half-Century long problem of addiction treatment disparities. We cannot imagine addressing disparities in addiction treatment without first acknowledging and deconstructing the etiology of this inequity. This article examines the history of addiction treatment disparities beginning with early twentieth-century drug policies. We begin by discussing structural racism, its contribution to treatment disparities, using opioid use disorder as a case study to highlight the importance of a structural competency framework in obtaining care. We conclude by discussing diversity in the workforce as an additional tool to minimizing disparities. Addiction treatment should be aimed at addressing care delivery in the context of the social, economic, and political determinants of health, which require appreciation of their historical origins to move toward equitable treatment.

A phenylalanine (Phe)-restricted diet is indispensable to control the blood Phe for individuals with phenylketonuria (PKU), who are also confronted with progressive bone impairment. Thus, the development of a low-Phe protein substitute that could positively regulate bone metabolism is desired for their bone health. Our previous study reported the preparation of a low-Phe containing whey hydrolysate (LPH) from a selected whey protein hydrolysate (TAH). However, the effect of LPH on the bone status is unknown. In this study, we used an ovariectomized (OVX) mice model to evaluate the anti-osteoporotic potential of oral administration of whey protein concentrate (WPC, protein control), TAH, and LPH on bone physiology and bone metabolism. The results showed that after 12 weeks of treatment, the decreased bone mineral density, the deteriorated trabecular microarchitecture, and the reduced ultimate load due to ovariectomy were significantly attenuated by two whey protein hydrolysates (TAH and LPH); meanwhile, the body weight, uterine weight, bone composition, and the femoral elastic load of OVX mice had not been significantly affected by whey samples. In addition, LPH and TAH dual-regulated bone remodeling in OVX mice through triggering osteogenesis (promoted the expression of runt-related protein 2 (Runx2) and osteoformation markers) and inhibiting osteoresorption as well as inflammation. The modulated mitogen-activated protein kinase signaling and the inhibited nuclear factor κB signaling by LPH and TAH might relate to the dual-regulatory activities on bone. Overall, in the OVX mice model, LPH exerted higher osteoprotective potential than TAH of the same dose by activating the bone formation markers and inhibiting the inflammatory status. The current study demonstrated for the first time the potential use of a low-Phe whey hydrolysate, a protein substitute for PKU individuals, in the prevention of osteoporosis.

The transmission of certain bacteria from the mother's gut to the infant's gut via breast milk (BM) is critical for offspring's immune system development. Dysbiosis of the BM microbiota can be caused by a variety of reasons, which can be influenced by probiotics delivered via the enteromammary route. The goal of this study was to investigate the bacterium that can be transmitted from mother to infant's intestine during various lactation periods in 19 mother-child dyads. Bacterial transmission is most common during the colostrum phase when bacteria with certain amplicon sequence variants (ASVs) enter the newborn intestine and inhabit it permanently. We have established that anaerobic gut-associated bacteria, such as Faecalibacterium, Blautia and Lachnoclostridium, transfer from the mother to the infant's gut with lactation dependence using the idea of weighted transfer ratios. Streptococcus salivarius, Bifidobacterium longum, and Lactobacillus gasseri were transferred from the maternal gut to the BM, as well as from the BM to the newborn gut, dependent on different ASVs. These findings suggest that isolating key microorganisms from breast milk could be utilized to modify the microbiota of BM or newborns by giving the mother a probiotic or adding it to artificial milk to promote neonatal health.