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583 results on '"Grande, S."'

109. (1)H NMR detects different metabolic profiles in glioblastoma stem-like cells

Author

Guidoni, L, Ricci-Vitiani, L, Rosi, A, Palma, A, Grande, S, Luciani, Am, Pelacchi, F, Di Martino, Susanna, Colosimo, C, Biffoni, M, De Maria Marchiano, Ruggero, Pallini, Roberto, Viti, V., di Martino S, De Maria Marchiano R (ORCID:0000-0003-2255-0583), Pallini R (ORCID:0000-0002-4611-8827), Guidoni, L, Ricci-Vitiani, L, Rosi, A, Palma, A, Grande, S, Luciani, Am, Pelacchi, F, Di Martino, Susanna, Colosimo, C, Biffoni, M, De Maria Marchiano, Ruggero, Pallini, Roberto, Viti, V., di Martino S, De Maria Marchiano R (ORCID:0000-0003-2255-0583), and Pallini R (ORCID:0000-0002-4611-8827)

Abstract

The metabolic profiles of glioblastoma stem-like cells (GSCs) growing in neurospheres were examined by 1H NMR spec- troscopy. Spectra of two GSC lines, labelled 1 and 83, from tumours close to the subventricular zone of the temporal lobe were studied in detail and compared with those of neural stem/progenitor cells from the adult olfactory bulb (OB-NPCs) and of the T98G glioblastoma cell line. In both GSCs, signals from myoinositol (Myo-I), UDP-hexosamines (UDP-Hex) and glycine indicated an astrocyte/glioma metabolism. For line 1, the presence of signals from N-acetyl aspartate, GABA and creatine pointed to a neuronal fingerprint. These metabolites were almost absent from line 83 spectra, whereas lipid signals, absent from normal neural lineages, were intense in line 83 spectra and remained low in those of line 1, irrespective of apoptotic fate. Spectra of OB-NPC cells displayed strong similarities with those from line 1, with low lipid signals and clearly detectable neuronal signals. In contrast, the spectral profile of line 83 was more similar to that of T98G, displaying high lipids and nearly complete absence of the neuronal markers. A mixed neural–astrocyte metabolic phenotype with a strong neuronal fingerprint was therefore found in line 1, while an astrocytic/glioma-like metabolism prevailed in line 83. We found a signal assigned to the amide proton of N-acetyl galactosamine in GSC lines and in OB-NPC spectra, whereas it was absent from those of T98G cells. This signal may be related to a stem-cell-specific protein glycosylation pattern and is therefore suggested as a marker of cell multipotency. Other GSC lines from patients with different clinical outcomes were then examined. Unsupervised analysis of spectral data from 13 lines yielded two clusters, with six lines resembling spectral features of line 1 and seven resembling those of line 83, suggesting that distinct metabolic phenotypes may be present in GSC lines

Published
2013

110. (1) H NMR detects different metabolic profiles in glioblastoma stem-like cells

Author

Guidoni, L, Ricci Vitiani, L, Rosi, A, Palma, A, Grande, S, Luciani, Am, Pelacchi, F, Di Martino, S, Colosimo, Cesare, Biffoni, M, De Maria, R, Pallini, R, Viti, V., Colosimo, Cesare (ORCID:0000-0003-3800-3648), Guidoni, L, Ricci Vitiani, L, Rosi, A, Palma, A, Grande, S, Luciani, Am, Pelacchi, F, Di Martino, S, Colosimo, Cesare, Biffoni, M, De Maria, R, Pallini, R, Viti, V., and Colosimo, Cesare (ORCID:0000-0003-3800-3648)

Abstract

The metabolic profiles of glioblastoma stem-like cells (GSCs) growing in neurospheres were examined by (1) H NMR spectroscopy. Spectra of two GSC lines, labelled 1 and 83, from tumours close to the subventricular zone of the temporal lobe were studied in detail and compared with those of neural stem/progenitor cells from the adult olfactory bulb (OB-NPCs) and of the T98G glioblastoma cell line. In both GSCs, signals from myoinositol (Myo-I), UDP-hexosamines (UDP-Hex) and glycine indicated an astrocyte/glioma metabolism. For line 1, the presence of signals from N-acetyl aspartate, GABA and creatine pointed to a neuronal fingerprint. These metabolites were almost absent from line 83 spectra, whereas lipid signals, absent from normal neural lineages, were intense in line 83 spectra and remained low in those of line 1, irrespective of apoptotic fate. Spectra of OB-NPC cells displayed strong similarities with those from line 1, with low lipid signals and clearly detectable neuronal signals. In contrast, the spectral profile of line 83 was more similar to that of T98G, displaying high lipids and nearly complete absence of the neuronal markers. A mixed neural-astrocyte metabolic phenotype with a strong neuronal fingerprint was therefore found in line 1, while an astrocytic/glioma-like metabolism prevailed in line 83. We found a signal assigned to the amide proton of N-acetyl galactosamine in GSC lines and in OB-NPC spectra, whereas it was absent from those of T98G cells. This signal may be related to a stem-cell-specific protein glycosylation pattern and is therefore suggested as a marker of cell multipotency. Other GSC lines from patients with different clinical outcomes were then examined. Unsupervised analysis of spectral data from 13 lines yielded two clusters, with six lines resembling spectral features of line 1 and seven resembling those of line 83, suggesting that distinct metabolic phenotypes may be present in GSC lines. Copyright © 2013 John Wiley & Sons, Ltd.

Published
2013

119. E29 International cooperation in endourology: Ureteral access sheath utility during flexible ureteroscopy for lower pole kidney stones

Author

Sanguedolce, F., primary, Cracco, C., additional, Grande, S., additional, Scoffone, C., additional, Kallidonis, P., additional, Liatsikos, E., additional, Sabockis, R., additional, Brehmer, M., additional, Jessen, J., additional, Knoll, T., additional, Franke, M., additional, Osther, P.J.S., additional, Traxer, O., additional, Hermann, T., additional, Merseburger, A., additional, Nagele, U., additional, Millan, F., additional, and Breda, A., additional

Published
2011
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120. E61 International cooperation in endourology: Supine vs. prone percutaneous nephrolithotomy in obese patients

Author

Sanguedolce, F., primary, Cracco, C., additional, Grande, S., additional, Scoffone, C., additional, Kallidonis, P., additional, Liatsikos, E., additional, Sabockis, R., additional, Brehmer, M., additional, Jessen, J., additional, Knoll, T., additional, Franke, M., additional, Traxer, O., additional, Hermann, T., additional, Merseburger, A., additional, Nagele, U., additional, Millan, F., additional, and Breda, A., additional

Published
2011
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128. NK Cytotoxicity Is Decreased During the Early Posttransplant Period Following Pediatric Allogeneic Transplantation From Unrelated Donors Using CD3/CD19-Depleted Graft.

Author

Pérez-Martínez, Antonio, primary, Ramírez, Manuel, additional, Ruiz-Salmerón, María, additional, Gonzalez-Vicent, Marta, additional, Grande, S., additional, Lassaletta, Alvaro, additional, Sevilla, Juliáan, additional, Tomas, Jose Francisco, additional, Madero, Luis, additional, and Diaz, Miguel Angel, additional

Published
2009
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134. Therapeutic Compliance and Efficacy of Dose-Dense R-CHOP-14 Immunochemotherapy Plus Pegfilgrastim for First-Line Treatment of Low Risk Diffuse Large B-Cell Lymphoma Patients: Preliminary Results of An Open-Label Clinical Trial in Spain

Author

Gonzalez-Barca, Eva, primary, Canales, Miguel, primary, Salar, Antonio, primary, Ferrer, Secundino, primary, Bargay, Joan, primary, Grande, S., primary, Gardella, Santiago, primary, Vidal, María Jesús, primary, Oriol, Albert, primary, López, A., primary, García, J., primary, Bello, Jl, primary, Tomás, Fj, primary, Briones, Javier, primary, and Caballero, Dolores, primary

Published
2008
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143. ENDOSCOPIC COMBINED INTRA-RENAL SURGERY (ECIRS) FOR THE TREATMENT OF COMPLEX UROLOGICAL PATHOLOGIES USING GALDAKAO-MODIFIED SUPINE VALDIVIA POSITION

Author

Scoffone, C., primary, Morra, I., additional, Cracco, C., additional, Ragni, F., additional, Poggio, M., additional, Cossu, M., additional, Vaccino, D., additional, Billia, M., additional, Grande, S., additional, Scarpa, R.M., additional, Ibarluzea, G., additional, and Valdivia Uria, J.G., additional

Published
2008
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