101. Production and effect of aldonic acids during enzymatic hydrolysis of lignocellulose at high dry matter content
- Author
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Henning Jørgensen, David Cannella, Claus Felby, and Chia-Wen Carmen Hsieh
- Subjects
Cellulose oxidation ,High solids hydrolysis ,lcsh:Biotechnology ,Gluconic acid ,Cellobiose ,Cellulase ,Management, Monitoring, Policy and Law ,Applied Microbiology and Biotechnology ,lcsh:Fuel ,chemistry.chemical_compound ,Hydrolysis ,lcsh:TP315-360 ,lcsh:TP248.13-248.65 ,Enzymatic hydrolysis ,Cellodextrin ,GH61 ,Organic chemistry ,Cellulose ,biology ,Renewable Energy, Sustainability and the Environment ,Beta-glucosidase ,Research ,Sciences bio-médicales et agricoles ,General Energy ,chemistry ,Biochemistry ,biology.protein ,Biotechnology - Abstract
The recent discovery of accessory proteins that boost cellulose hydrolysis has increased the economical and technical efficiency of processing cellulose to bioethanol. Oxidative enzymes (e.g. GH61) present in new commercial enzyme preparations have shown to increase cellulose conversion yields. When using pure cellulose substrates it has been determined that both oxidized and unoxidized cellodextrin products are formed. We report the effect of oxidative activity in a commercial enzyme mix (Cellic CTec2) upon overall hydrolysis, formation of oxidized products and impact on β-glucosidase activity. The experiments were done at high solids loadings using a lignocellulosic substrate simulating commercially relevant conditions., info:eu-repo/semantics/published
- Published
- 2012
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