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749 results on '"Hirai, Makoto"'

102. MOESM5 of Plasmodium Rab5b is secreted to the cytoplasmic face of the tubovesicular network in infected red blood cells together with N-acylated adenylate kinase 2

Author

Ebine, Kazuo, Hirai, Makoto, Miako Sakaguchi, Yahata, Kazuhide, Kaneko, Osamu, and Saito-Nakano, Yumiko

Abstract

Additional file 5. No growth defect by the expression of PfRab5b-YFP-DD or PfRab5bQ94L-YFP-DD seen in the growth curve. Growth curve of parasites expressing PfRab5b-YFP-DD (a) or PfRab5bQ94L-YFP-DD (b). Parasitemia (%) was measured after treatment with (green and yellow) or without (blue and red) 0.5 μM Shld1 in the presence of 5 nM WR99210-HCl. (c) Immunoblot using anti-GFP antibody to probe PfRab5-YFP-DD showed that PfRab5-YFP-DD was highly expressed after the incubation with 0.5 μM of Shld1 for 24 h. Tpx1 is a loading control. (d) Quantification of intensity of PfRab5-YFP-DD in Additional Figure 5c. (e) Immunoblot of PfRab5-YFP-DD before or after washout of Shld1 for 2 h. (f) Quantification of intensity of PfRab5-YFP-DD in Additional Figure 5e.

Published
2016
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103. Corrigendum: Plasmodium berghei ANKA causes intestinal malaria associated with dysbiosis

Author

Taniguchi, Tomoyo, Miyauchi, Eiji, Nakamura, Shota, Hirai, Makoto, Suzue, Kazutomo, Imai, Takashi, Nomura, Takahiro, Handa, Tadashi, Okada, Hiroko, Shimokawa, Chikako, Onishi, Risa, Olia, Alex, Hirata, Jun, Tomita, Haruyoshi, Ohno, Hiroshi, Horii, Toshihiro, and Hisaeda, Hajime

Subjects
DNA, Bacterial, Male, Mice, Inbred BALB C, Plasmodium berghei, Microbiota, Firmicutes, Corrigenda, Malaria, Intestines, Mice, Inbred C57BL, Feces, Mice, Blood-Brain Barrier, RNA, Ribosomal, 16S, Databases, Genetic, Proteobacteria, Animals, Dysbiosis
Abstract

Gastrointestinal symptoms, such as abdominal pain and diarrhea, are frequently observed in patients with Plasmodium falciparum malaria. However, the correlation between malaria intestinal pathology and intestinal microbiota has not been investigated. In the present study, infection of C57BL/6 mice with P. berghei ANKA (PbA) caused intestinal pathological changes, such as detachment of epithelia in the small intestines and increased intestinal permeability, which correlated with development with experimental cerebral malaria (ECM). Notably, an apparent dysbiosis occurred, characterized by a reduction of Firmicutes and an increase in Proteobacteria. Furthermore, some genera of microbiota correlated with parasite growth and/or ECM development. By contrast, BALB/c mice are resistant to ECM and exhibit milder intestinal pathology and dysbiosis. These results indicate that the severity of cerebral and intestinal pathology coincides with the degree of alteration in microbiota. This is the first report demonstrating that malaria affects intestinal microbiota and causes dysbiosis.

Published
2016

104. Study on attenuation of sound by droplets

Author

Shimizu, Taro, Hirai, Makoto, Tsutsumi, Seiji, Takaki, Ryoji, and Arita, Makoto

Subjects
Fluid Dynamics, Multi-Phase Flow, Sound Attenuation
Abstract

資料番号: PAIS08015000

Published
2009

105. Impaired renal function is associated with recurrence after cryoballoon catheter ablation for paroxysmal atrial fibrillation: A potential effect of non-pulmonary vein foci

Author

Yanagisawa, Satoshi, primary, Inden, Yasuya, additional, Kato, Hiroyuki, additional, Fujii, Aya, additional, Mizutani, Yoshiaki, additional, Ito, Tadahiro, additional, Kamikubo, Yosuke, additional, Kanzaki, Yasunori, additional, Ando, Monami, additional, Hirai, Makoto, additional, Shibata, Rei, additional, and Murohara, Toyoaki, additional

Published
2017
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108. Previous residences and characteristics of residents in a retirement home in Onjuku town, Chiba prefecture

Author

Hirai, Makoto

Abstract

This paper intends to consider the elderly migration to retirement homes for the elderly (Yuryo-Rojin-Homu in Japanese) as a case study in Onjuku in Chiba prefecture. To achive this purpose, the attributes of 286 residents and their previous residences are analyzed based on the directory of residents. As the resultes, the attributes of residents are mostly uniform. Most of them moved independently at ages of 60s from central area of the Tokyo Metropolitan Area (Tokyo, Yokohama, Kawaswaki), and their moves were done by themselves or with spouses. As to their social-economic statuses, their previous occupations and educational status are very high. The intensions of their movements are not to enjoy their life after the retirement at the facility. For residents, most significant function of the private home is the nursing care that is secured until they die, namely "lifelong care". Their movements are not the "amenity move". It is obvious that the private home residents move to receive the "lifelong care".

Published
2004

109. PPL based on Trigonometric Smoothing Functions as Activation Functions of Hidden Units

Author

Wada, Koji, Hirai, Makoto, Nakajima, Shigeyoshi, and Okamoto, Jiro

Subjects
smoothing function, neural network, PPL, parameter number, parity discrimination, trigonometric function, learning speed
Abstract

Recently Projection Pursuit Learning (PPL) emerged in the Neural Network (NN) literatures in recent years which used smoothing functions as activation functions in hidden units of NN. As this network uses smoothing functions as activation functions for hidden units, adaptability for various problems is expected to be excellent than other networks....

Published
2000

110. An Adaptive Speech Enhancement Method According to Noise Intensity Based on an Auditory Model

Author

Choi, Jae-seung, Hirai, Makoto, Nakajima, Shigeyoshi, Hosokawa, Shoichi, and Okamoto, Jiro

Subjects
Estimation of noise intensity, Lateral inhibition, Noise reduction, Adaptive speech enhancement, Neural network
Abstract

In speech recognition under noisy environments, it is necessary to construct a system which reduces noise and enhances speech. It is effective to imitate the human auditory system which has an excellent mechanism to analyze the spectrum for speech enhancement....

Published
1999

112. Correction: Corrigendum: Plasmodium berghei ANKA causes intestinal malaria associated with dysbiosis

Author

Taniguchi, Tomoyo, primary, Miyauchi, Eiji, additional, Nakamura, Shota, additional, Hirai, Makoto, additional, Suzue, Kazutomo, additional, Imai, Takashi, additional, Nomura, Takahiro, additional, Handa, Tadashi, additional, Okada, Hiroko, additional, Shimokawa, Chikako, additional, Onishi, Risa, additional, Olia, Alex, additional, Hirata, Jun, additional, Tomita, Haruyoshi, additional, Ohno, Hiroshi, additional, Horii, Toshihiro, additional, and Hisaeda, Hajime, additional

Published
2016
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113. Elevated Red Blood Cell Distribution Width Predicts Recurrence After Catheter Ablation for Atrial Fibrillation in Patients With Heart Failure – Comparison With Non-Heart Failure Patients –

Author

Yanagisawa, Satoshi, primary, Inden, Yasuya, additional, Kato, Hiroyuki, additional, Miyoshi, Aya, additional, Mizutani, Yoshiaki, additional, Ito, Tadahiro, additional, Kamikubo, Yosuke, additional, Kanzaki, Yasunori, additional, Hirai, Makoto, additional, and Murohara, Toyoaki, additional

Published
2016
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117. Plasmodium berghei ANKA causes intestinal malaria associated with dysbiosis

Author

Taniguchi, Tomoyo, primary, Miyauchi, Eiji, additional, Nakamura, Shota, additional, Hirai, Makoto, additional, Suzue, Kazutomo, additional, Imai, Takashi, additional, Nomura, Takahiro, additional, Handa, Tadashi, additional, Okada, Hiroko, additional, Shimokawa, Chikako, additional, Onishi, Risa, additional, Olia, Alex, additional, Hirata, Jun, additional, Tomita, Haruyoshi, additional, Ohno, Hiroshi, additional, Horii, Toshihiro, additional, and Hisaeda, Hajime, additional

Published
2015
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118. Body mass index is associated with prognosis in Japanese elderly patients with atrial fibrillation: an observational study from the outpatient clinic

Author

Yanagisawa, Satoshi, primary, Inden, Yasuya, additional, Yoshida, Naoki, additional, Kato, Hiroyuki, additional, Miyoshi-Fujii, Aya, additional, Mizutani, Yoshiaki, additional, Ito, Tadahiro, additional, Kamikubo, Yosuke, additional, Kanzaki, Yasunori, additional, Hirai, Makoto, additional, and Murohara, Toyoaki, additional

Published
2015
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119. Production of a transgenic mosquito expressing circumsporozoite protein, a malarial protein, in the salivary gland of Anopheles stephensi (Diptera: Culicidae)

Author

Matsuoka, Hiroyuki, Ikezawa, Tsunetaka, and Hirai, Makoto

Subjects
Mice, Inbred BALB C, fungi, Green Fluorescent Proteins, malaria, Protozoan Proteins, Antibodies, Protozoan, salivary gland, transgenic mosquito, Salivary Glands, flying syringe, Animals, Genetically Modified, Mice, vaccine, parasitic diseases, Anopheles, Malaria Vaccines, Animals, Female, Plasmids
Abstract

We are producing a transgenic mosquito, a flying syringe, to deliver a vaccine protein to human beings via the saliva the mosquito deposits in the skin while biting. The mosquito produces a vaccine protein in the salivary gland (SG) and deposits the protein into the host's skin when it takes the host's blood. We chose circumsporozoite protein (CSP), currently the most promising malaria vaccine candidate, to be expressed in the SG of Anopheles stephensi. To transform the mosquitoes, plasmid containing the CSP gene under the promoter of female SG-specific gene, as well as the green fluorescent protein (GFP) gene under the promoter of 3xP3 as a selection marker in the eyes, was injected into more than 400 eggs. As a result, five strains of GFP-expressing mosquitoes were established, and successful CSP expression in the SG was confirmed in one strain. The estimated amount of CSP in the SG of the strain was 40 ng per mosquito. We allowed the CSP-expressing mosquitoes to feed on mice to induce the production of anti-CSP antibody. However, the mice did not develop anti-CSP antibody even after transgenic mosquitoes had bitten them several times. We consider that CSP in the SG was not secreted properly into the saliva. Further techniques and trials are required in order to realize vaccine-delivering mosquitoes.

Published
2010

120. Differences in activated clotting time among uninterrupted anticoagulants during the periprocedural period of atrial fibrillation ablation

Author

Nagao, Tomoyuki, primary, Inden, Yasuya, additional, Yanagisawa, Satoshi, additional, Kato, Hiroyuki, additional, Ishikawa, Shinji, additional, Okumura, Satoshi, additional, Mizutani, Yoshiaki, additional, Ito, Tadahiro, additional, Yamamoto, Toshihiko, additional, Yoshida, Naoki, additional, Hirai, Makoto, additional, and Murohara, Toyoaki, additional

Published
2015
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124. Direct evidence for the atovaquone action on the Plasmodium cytochrome bc 1 complex

Author

Siregar, Josephine E., primary, Kurisu, Genji, additional, Kobayashi, Tamaki, additional, Matsuzaki, Motomichi, additional, Sakamoto, Kimitoshi, additional, Mi-ichi, Fumika, additional, Watanabe, Yoh-ichi, additional, Hirai, Makoto, additional, Matsuoka, Hiroyuki, additional, Syafruddin, Din, additional, Marzuki, Sangkot, additional, and Kita, Kiyoshi, additional

Published
2015
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125. Feasibility and Safety of Uninterrupted Dabigatran Therapy in Patients Undergoing Ablation for Atrial Fibrillation

Author

Nagao, Tomoyuki, primary, Inden, Yasuya, additional, Shimano, Masayuki, additional, Fujita, Masaya, additional, Yanagisawa, Satoshi, additional, Kato, Hiroyuki, additional, Ishikawa, Shinji, additional, Miyoshi, Aya, additional, Okumura, Satoshi, additional, Ohguchi, Shiou, additional, Yamamoto, Toshihiko, additional, Yoshida, Naoki, additional, Hirai, Makoto, additional, and Murohara, Toyoaki, additional

Published
2015
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128. Efficacy and Safety of Apixaban in the Patients Undergoing the Ablation of Atrial Fibrillation

Author

NAGAO, TOMOYUKI, primary, INDEN, YASUYA, additional, SHIMANO, MASAYUKI, additional, FUJITA, MASAYA, additional, YANAGISAWA, SATOSHI, additional, KATO, HIROYUKI, additional, ISHIKAWA, SHINJI, additional, MIYOSHI, AYA, additional, OKUMURA, SATOSHI, additional, OHGUCHI, SHIOU, additional, YAMAMOTO, TOSHIHIKO, additional, YOSHIDA, NAOKI, additional, HIRAI, MAKOTO, additional, and MUROHARA, TOYOAKI, additional

Published
2014
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129. A simple algorithm for localizing accessory pathways in patients with Wolff-Parkinson-White syndrome using only the R/S ratio

Author

Taguchi, Noriko, primary, Yoshida, Naoki, additional, Inden, Yasuya, additional, Yamamoto, Toshihiko, additional, Miyata, Shinjiro, additional, Fujita, Masaya, additional, Yokoi, Kenichiro, additional, Kyo, Seifuku, additional, Shimano, Masayuki, additional, Hirai, Makoto, additional, and Murohara, Toyoaki, additional

Published
2014
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130. Abstract 11069: Alogliptin, a Dipeptidyl Peptidase-4 Inhibitor Improves Ventricular Tachypacing-Induced Atrial Fibrillation in a Rabbit Model

Author

Yamamoto, Toshihiko, primary, Shimano, Masayuki, additional, Inden, Yasuya, additional, Yoshida, Naoki, additional, Ishikawa, Shinji, additional, Okumura, Satoshi, additional, Kato, Hiroyuki, additional, Yanagisawa, Satoshi, additional, Nagao, Tomoyuki, additional, Ito, Tadahiro, additional, Mizutani, Yoshiaki, additional, Tsuji, Yukiomi, additional, Hirai, Makoto, additional, and Murohara, Toyoaki, additional

Published
2014
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134. Generation of Rodent Malaria Parasites with a High Mutation Rate by Destructing Proofreading Activity of DNA Polymerase δ

Author

Honma, Hajime, primary, Hirai, Makoto, additional, Nakamura, Shota, additional, Hakimi, Hassan, additional, Kawazu, Shin-ichiro, additional, Palacpac, Nirianne M.Q., additional, Hisaeda, Hajime, additional, Matsuoka, Hiroyuki, additional, Kawai, Satoru, additional, Endo, Hiroyoshi, additional, Yasunaga, Teruo, additional, Ohashi, Jun, additional, Mita, Toshihiro, additional, Horii, Toshihiro, additional, Furusawa, Mitsuru, additional, and Tanabe, Kazuyuki, additional

Published
2014
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137. Clinical Outcome of Implantable Cardioverter Defibrillators With Recalled and Non-Recalled Leads in Japanese Patients

Author

Yanagisawa, Satoshi, primary, Inden, Yasuya, additional, Shimano, Masayuki, additional, Yoshida, Naoki, additional, Ichiyanagi, Hiroshi, additional, Fujita, Masaya, additional, Ohguchi, Shiou, additional, Ishikawa, Shinji, additional, Kato, Hiroyuki, additional, Okumura, Satoshi, additional, Miyoshi, Aya, additional, Nagao, Tomoyuki, additional, Yamamoto, Toshihiko, additional, Hirai, Makoto, additional, and Murohara, Toyoaki, additional

Published
2014
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143. Comparison of vectorcardiographic and 12-lead electrocardiographic detections of abnormalities in repolarization properties due to preexcitation in patients with Wolff-Parkinson-White syndrome: proposal of a novel concept of a 'remodeling gradient'

Author

Shimizu Atsuya, Sugino Mikio, Tsuboi Naoya, Sawada Takahisa, Hirayama Haruo, Inden Yasuya, Saito Hidehiko, Kondo Takahisa, Hirai Makoto, Yoshida Yukihiko, Akahoshi Makoto, Ito Teruo, and Iino Shigeo

Subjects
Adult, Male, medicine.medical_specialty, Heart disease, Adolescent, Radiofrequency ablation, medicine.medical_treatment, Vectorcardiography, Accessory pathway, law.invention, Electrocardiography, law, Heart Conduction System, Internal medicine, medicine, Repolarization, Humans, Aged, medicine.diagnostic_test, business.industry, Body Surface Potential Mapping, Middle Aged, Ablation, medicine.disease, medicine.anatomical_structure, Ventricle, Cardiology, Catheter Ablation, Female, Wolff-Parkinson-White Syndrome, Cardiology and Cardiovascular Medicine, business
Abstract

Repolarization abnormalities after radiofrequency ablation in patients with manifest Wolff-Parkinson-White syndrome (WPW) have been attributed to cardiac memory of pre-existing changes in repolarization properties. We compared spatial ventricular gradient (VG) from vectorcardiograms with QRST values of 12-lead ECG in 41 patients with WPW (group A, manifest WPW due to left-sided accessory pathway (n = 20); group B, manifest WPW due to right-sided accessory pathway (n = 12); group C, concealed WPW (n = 9)) before and after ablation. Group N (n = 607) served as control. In groups A and B, the abnormalities of spatial VG and QRST values of 12-lead ECG that existed before and 1 day after ablation significantly decreased 1 week after ablation. In group C, spatial VG and QRST values of 12-lead ECG showed no significant changes. The diagnostic ability of spatial VG is almost equivalent to that of the QRST value of ECG in detecting repolarization abnormalities in patients with WPW before and after ablation. We propose a new concept of a "remodeling gradient" directing from the preexcited area to the opposite side of the ventricle as a result of preexcitation-induced electrical remodeling.

Published
2000

149. Plasmodium falciparum kelch 13: a potential molecular marker for tackling artemisinin-resistant malaria parasites.

Author

Mita, Toshihiro, Tachibana, Shin-Ichiro, Hashimoto, Muneaki, and Hirai, Makoto

Abstract

Although artemisinin combination therapies have been deployed as a first-line treatment for uncomplicated malaria in almost all endemic countries, artemisinin-resistant parasites have emerged and have gradually spread across the Greater Mekong subregions. There is growing concern that the resistant parasites may migrate to or emerge indigenously in sub-Saharan Africa, which might provoke a global increase in malaria-associated morbidity and mortality. Therefore, development of molecular markers that enable identification of artemisinin resistance with high sensitivity is urgently required to combat this issue. In 2014, a potential artemisinin-resistance responsible gene, Plasmodium falciparum kelch13, was discovered. Here, we review the genetic features of P. falciparum kelch13 and discuss its related resistant mechanisms and potential as a molecular marker. [ABSTRACT FROM AUTHOR]

Published
2016
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