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379 results on '"Hou, Yanhui"'

104. Intranasal delivery of cationic liposome-protamine complex mRNA vaccine elicits effective anti-tumor immunity

Author

Shijie Ma, Jueshuo Guo, Yue Zhao, Mai Yaping, Hou Yanhui, and Jianhong Yang

Subjects
0301 basic medicine, Lung Neoplasms, medicine.medical_treatment, Immunology, Antigen presentation, Pharmacology, Biology, Lymphocyte Activation, Cancer Vaccines, Fatty Acids, Monounsaturated, Carcinoma, Lewis Lung, Mice, 03 medical and health sciences, 0302 clinical medicine, Immune system, Antigen, medicine, Animals, Humans, Cationic liposome, Protamines, RNA, Messenger, Administration, Intranasal, Keratin-19, Cell Differentiation, Dendritic Cells, Immunotherapy, Dendritic cell, Protamine, Tumor Burden, Mice, Inbred C57BL, Quaternary Ammonium Compounds, 030104 developmental biology, Liposomes, biology.protein, Female, Adjuvant, Neoplasm Transplantation, 030215 immunology
Abstract

Immunization with synthetic mRNA encoding tumor-associated antigens is an emerging vaccine strategy for the treatment of cancer. In order to prevent mRNA degradation, promote antigen-presenting cells antigen presentation, and induce an anti-tumor immune response, we investigated the nasal administration of mRNA vaccines with positively charged protamine to concentrate mRNA, form a stable polycation-mRNA complex, and encapsulate the complex with DOTAP/Chol/DSPE-PEG cationic liposomes. Cationic liposome/protamine complex (LPC) showed significantly greater efficiency in uptake of vaccine particles in vitro and stronger capacities to stimulate dendritic cell maturation, which further induced a potent anti-tumor immune response. Intranasal immunization of mice with cationic LPC containing mRNA encoding cytokeratin 19 provoked a strong cellular immune response and slowed tumor growth in an aggressive Lewis lung cancer model. The results of this study provide evidence that cationic LPC can be used as a safe and effective adjuvant and this mRNA formulation provides a basis for anti-cancer vaccination of humans.

Published
2020

120. Characterization of Cepharanthin Nanosuspensions and Evaluation of Their In VitroActivity for the HepG2 Hepatocellular Carcinoma Cell Line

Author

Zhao, Yue, Fu, Tingting, Meng, Gaoke, Qiao, Fangxia, Hou, Yanhui, Liu, Yanhua, and Yang, Jianhong

Abstract

Background: Conventional cancer therapeutics has enormous toxicity and severe side effects that generate multi-drug resistance. Therefore, an urgent need exists for new alternative therapeutic agents for cancer treatment. Cepharanthin (CEP) has anti-cancer potential but has poor aqueous solubility, which limits its clinical use. Nanosuspensions (NS) are attractive as insoluble drug delivery systems. Objectives: In this study, we used D-alpha Tocopherol acid Polyethylene Glycol Succinate (TPGS), Polyvinylpyrrolidone (PVP) VA64, and Croscamellose Sodium (CCS) as stabilizers to produce TPGS-CEP-NS, PVP VA64-CEP-NS, and CCS-CEP-NS by wet-milling technology, and then characterized the NS and evaluated their functional activities in vitro. Methods: CEP Nanosuspensions (CEP-NS) were prepared by the wet-milling method. The prepared NS were characterized by particle size distribution, zeta potential, morphology, surface properties, and molecular interactions. The NS were evaluated for their effects on HepG2 cells in vitro. The evaluations included assessment of cellular cytotoxicity, cellular apoptosis, NS uptake by cells, and mitochondrial membrane potential changes. Results: CEP-NS showed an appropriate particle size and were physically stable. All CEP-NS exhibited HepG2 enhanced anti-proliferative effects by reducing cell viability, enhanced cellular uptake, induced cellular apoptosis, and mitochondrial membrane potential loss. Conclusions: CEP-NS may be effective therapeutic agents for the treatment of hepatocellular carcinoma.

Published
2020
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127. Targeting strategies of liposomal subunit vaccine delivery systems to improve vaccine efficacy.

Author

Yu, Rui, Mai, Yaping, Zhao, Yue, Hou, Yanhui, Liu, Yanhua, and Yang, Jianhong

Subjects
VACCINE effectiveness, PATTERN perception receptors, VACCINES, IMMUNOLOGICAL adjuvants, IMMUNE system
Abstract

Liposomes are versatile delivery systems and immunological adjuvants that not only can load various antigens, such as proteins, peptides, nucleic acids and carbohydrates, but also can combine them with immunostimulators. Liposomes have great potential in the development of new types of vaccines, and much effort has been devoted to enhancing vaccine efficacy in recent years. Different types of immune cells such as macrophages and dendritic cells play an important role in the immune response and in preventing or treating cancer, allergy or many other infectious diseases. Targeting liposome-based delivery systems to certain immune cells and organs is one of the most effective measures in such treatments. Extensive research has shown that liposomes combined with immunostimulators or modified with pattern recognition receptor ligands can target various immune cells and the lymphatic system, thus not only inducing and promoting the desired immune response but also decreasing adverse effects throughout the body and avoiding targeting irrelevant cell types or tissues. Therefore, in this review, we outline some targeting strategies that can be adopted in the design of liposomal vaccines to improve vaccine efficacy, and we summarise the related liposome-based vaccine applications in several diseases. These applications have great potential to treat or prevent some infectious and intractable diseases. [ABSTRACT FROM AUTHOR]

Published
2019
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129. Fabrication of multicomponent amorphous bufadienolides nanosuspension with wet milling improves dissolution and stability.

Author

Zuo, Wenbao, Qu, Wenjing, Li, Na, Yu, Rui, Hou, Yanhui, Liu, Yanhua, Gou, Guojing, and Yang, Jianhong

Subjects
NANOMEDICINE, AMORPHOUS substances, EXCIPIENTS, PATIENT compliance, BUFADIENOLIDES, ANTINEOPLASTIC agents
Abstract

Multicomponent formulations have attracted increasing attention because of their favourable patient compliance and greater therapeutic effect. The aim of this study was to develop a multicomponent nanosuspension formulation of bufadienolides, the antitumor components of a traditional Chinese medicine, toad venom, using a wet-milling technique to improve its dissolution behaviour. Croscarmellose sodium (CCS) and sodium lauryl sulfate (SLS) were chosen as the combined stabilizers of the nanosuspension. A Taguchi orthogonal array design was used for this study to optimize the formulation and process parameters. The optimized nanosuspension was characterized by its particle size distribution, zeta potential, morphology, crystallinity, molecular interactions, stability and dissolution. The results showed that the nanosuspension was a homogeneous amorphous system with average particle sizes of <100 nm and significantly improved dissolution behaviour. It was also physically stable for at least 2 months; steric and kinetic stabilization were its main stability mechanisms. These findings suggested that the use of wet milling to fabricate nanosuspensions is a promising method for achieving the fast and synchronized dissolution of multicomponent formulations, presumably increasing the bioavailability of poorly water-soluble drugs. [ABSTRACT FROM AUTHOR]

Published
2018
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133. Study on assessment of the sustainable development of regional industrial enterprises

Author

Hou Yanhui, Yuan Qing-he, and Wang Xin-hua

Subjects
Sustainable development, Engineering, Index (economics), Index system, Management science, business.industry, Fuzzy set, Evaluation methods, Assessment index, Environmental economics, business, Fuzzy logic, Membership function
Abstract

Assessment of sustainable development of regional industry enterprises is an important content of regional sustainable development study. Based on the principles of establishing assessment index system of sustainable development of regional industrial enterprises, an index system including four-layer and 56 indicators was established, the membership function model of quantitative indexes and the choosing value standards of qualitative indexes were discussed. Based on the learning mechanism of BP neural network, the weight of each index and each layer were ultimately determined. In the end, the fuzzy comprehensive evaluation method of comprehensive assessment and a practical verifying example were given. This study can provide scientific basis for working out strategy of sustainable development of regional industrial enterprises.

Published
2009

141. Development and evaluation of alginate-chitosan gastric floating beads loading with oxymatrine solid dispersion.

Author

Liu, Yanhua, Chen, Lihong, Zhou, Chengming, Yang, Jianhong, Hou, Yanhui, and Wang, Wenping

Subjects
DRUG development, CHITOSAN, ORAL drug administration, DISPERSION (Chemistry), PHARMACOLOGY, BIOAVAILABILITY, SCANNING electron microscopes
Abstract

Oxymatrine (OM) can be metabolized to matrine in gastrointestinal ileocecal valve after oral administration, which affects pharmacological activity and reduce bioavailability of OM. A type of multiple-unit alginate-chitosan (Alg-Cs) floating beads was prepared by the ionotropic gelation method for gastroretention delivery of OM. A solid dispersion technique was applied and incorporated into beads to enhance the OM encapsulation efficiency (EE) and sustain the drug release. The surface morphology and internal hollow structure of beads were evaluated using optical microscopy and scanning electron microscopy (SEM). The developed Alg-Cs beads were spherical in shape with hollow internal structure and had particle size of 3.49 ± 0.09 mm and 1.33 ± 0.09 mm for wet and dried beads. Over 84% of the optimized OM solid dispersion-loaded Alg-Cs beads were able to continuously float over the simulated gastric fluid for 12 hin vitro. The OM solid dispersion-loaded Alg-Cs beads showed drug EE of 67.07%, which was much higher than that of beads loading with pure OM. Compared with the immediate release of OM capsules and pure OM-loaded beads, the release of OM from solid dispersion-loaded Alg-Cs beads was in a sustained-release manner for 12 h. Prolonged gastric retention time of over 8.5 h was achieved for OM solid dispersion-loaded Alg-Cs floating beads in healthy rabbit inin vivofloating ability evaluated by X-ray imaging. The developed Alg-Cs beads loading with OM solid dispersion displayed excellent performance features characterized by excellent gastric floating ability, high drug EE and sustained-release pattern. The study illustrated the potential use of Alg-Cs floating beads combined with the solid dispersion technique for prolonging gastric retention and sustaining release of OM, which could provide a promising drug delivery system for gastric-specific delivery of OM for bioavailability enhancement. [ABSTRACT FROM AUTHOR]

Published
2016
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