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Your search keyword '"Jakubowski, Hieronim"' showing total 614 results
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614 results on '"Jakubowski, Hieronim"'

106. Modification by homocysteine thiolactone affects redox status of cytochrome c

Author

Perla-Kajan, Joanna, Marczak, Lukasz, Kajan, Laszlo, Skowronek, Pawel, Twardowski, Tomasz, and Jakubowski, Hieronim

Subjects
Circular dichroism -- Analysis, Cytochrome c -- Structure, Cytochrome c -- Spectra, Homocysteine -- Structure, Homocysteine -- Spectra, Proteolysis -- Analysis, Redox potential -- Analysis, Biological sciences, Chemistry
Abstract

Research study conducted shows that N-HGcy-cytochrome c is more resistant to proteolytic degradation by trypsin, chymotrypsin and Pronase than the native counterpart. N-homocysteinylation of susceptible lysine residues in cytochrome c has important structural and functional consequences.

Published
2007

108. Labeled EF-Tus for rapid kinetic studies of pretranslocation complex formation

Author

Liu, Wei, Kavaliauskas, Darius, Schrader, Jared M., Poruri, Kiran, Birkedal, Victoria, Goldman, Emanuel, Jakubowski, Hieronim, Mandecki, Wlodek, Uhlenbeck, Olke C., Knudsen, Charlotte R., Goldman, Yale E., and Cooperman, Barry S.

Abstract

The universally conserved translation elongation factor EF-Tu delivers aminoacyl(aa)-tRNA in the form of an aa-tRNA·EF-Tu·GTP ternary complex (TC) to the ribosome where it binds to the cognate mRNA codon within the ribosomal A-site, leading to formation of a pretranslocation (PRE) complex. Here we describe preparation of QSY9 and Cy5 derivatives of the variant E348C-EF-Tu that are functional in translation elongation. Together with fluorophore derivatives of aa-tRNA and of ribosomal protein L11, located within the GTPase associated center (GAC), these labeled EF-Tus allow development of two new FRET assays that permit the dynamics of distance changes between EF-Tu and both L11 (Tu-L11 assay) and aa-tRNA (Tu-tRNA assay) to be determined during the decoding process. We use these assays to examine: (i) the relative rates of EF-Tu movement away from the GAC and from aa-tRNA during decoding, (ii) the effects of the misreading-inducing antibiotics streptomycin and paromomycin on tRNA selection at the A-site, and (iii) how strengthening the binding of aa-tRNA to EF-Tu affects the rate of EF-Tu movement away from L11 on the ribosome. These FRET assays have the potential to be adapted for high throughput screening of ribosomal antibiotics.

Published
2014

110. Methylfolate Trap Promotes Bacterial Thymineless Death by Sulfa Drugs

Author

Guzzo, Marissa B., primary, Nguyen, Hoa T., additional, Pham, Thanh H., additional, Wyszczelska-Rokiel, Monika, additional, Jakubowski, Hieronim, additional, Wolff, Kerstin A., additional, Ogwang, Sam, additional, Timpona, Joseph L., additional, Gogula, Soumya, additional, Jacobs, Michael R., additional, Ruetz, Markus, additional, Kräutler, Bernhard, additional, Jacobsen, Donald W., additional, Zhang, Guo-Fang, additional, and Nguyen, Liem, additional

Published
2016
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117. Aggregation and structural changes of alpha(S1)-, beta- andkappa-caseins induced by homocysteinylation

Author

Stroylova, Yulia, Zimny, Jaroslaw, Yousefi, Reza, Chobert, Jean-Marc, Jakubowski, Hieronim, Muronetz, Vladimir I., Haertlé, Thomas, Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de la Recherche Agronomique (INRA), Lomonosov Moscow State University (MSU), Agricultural University, Shiraz University (Shiraz University ), Polish Academy of Sciences (PAN), University of Medecine and Dentistry of New Jersey, Partenaires INRAE, French Embassy, Region Pays de Loire, Russian Foundation for Basic Researches [08-04-00231-a, 09-04-92740-NNIOM_a], and DFG International Research Training Group [GRK 1563]

Subjects
Homocysteinylation, NEURODEGENERATIVE DISEASES, Amyloid structures, DEMENTIA, Casein, CONGO RED, FIBRIL FORMATION, Intrinsically unstructured proteins, CIRCULAR-DICHROISM, ALZHEIMERS-DISEASE, Aggregation, [SDV.IDA]Life Sciences [q-bio]/Food engineering, BOVINE, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, FLUORESCENCE, SULFONATE
Abstract

Elevated homocysteine levels are resulting in N-homocysteinylation of lysyl residues in proteins and they correlate with a number of human pathologies. However, the role of N-homocysteinylation of lysyl residues is still poorly known. In order to study the features of homocysteinylation of intrinsically unstructured proteins (IUP) bovine caseins were used as a model. alpha(S1)-, beta- and kappa-caseins, showing different aggregations and micelle formation, were modified with homocysteine-thiolactone and their physico-chemical properties were studied. Efficiency of homocysteine incorporation was estimated to be about 1.5.2.1 and 1.3 homocysteyl residues per one beta-, alpha(S1)-, and kappa-casein molecule, respectively. Use of intrinsic and extrinsic fluorescent markers such as Trp, thioflavin T and ANS, reveal structural changes of casein structures after homocysteinylation reflected by an increase in beta-sheet content, which in some cases may be characteristic of amyloid-like transformations. CD spectra also show an increase in beta-sheet content of homocysteinylated caseins. Casein homocysteinylation leads in all cases to aggregation. The sizes of aggregates and aggregation rates were dependent on homocysteine thiolactone concentration and temperature. DES and microscopic studies have revealed the formation of large aggregates of about 1-3 mu m. Homocysteinylation of alpha(S1)- and beta-caseins results in formation of regular spheres. Homocysteinylated kappa-casein forms thin unbranched fibrils about 400-800 nm long. In case of kappa-casein amyloidogenic effect of homocysteinylation was confirmed by Congo red spectra. Taken together, data indicate that N-homocysteinylation provokes significant changes in properties of native caseins. A comparison of amyloidogenic transformation of 3 different casein types, belonging to the IUP protein family, shows that the efficiency of amyloidogenic transformation upon homocysteinylation depends on micellization capacity, additional disulphide bonds and other structural features. (C) 2011 Elsevier B.V. All rights reserved.

Published
2011

126. Labeled EF-Tus for Rapid Kinetic Studies of Pretranslocation Complex Formation

Author

Liu, Wei, primary, Kavaliauskas, Darius, additional, Schrader, Jared M., additional, Poruri, Kiran, additional, Birkedal, Victoria, additional, Goldman, Emanuel, additional, Jakubowski, Hieronim, additional, Mandecki, Wlodek, additional, Uhlenbeck, Olke C., additional, Knudsen, Charlotte R., additional, Goldman, Yale E., additional, and Cooperman, Barry S., additional

Published
2014
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134. Effects of Endurance and Endurance-strength Exercise on Renal Function in Abdominally Obese Women with Renal Hyperfiltration: A Prospective Randomized Trial.

Author

Szulinska, Monika, Skrypnik, Damian, Ratajczak, Marzena, Karolkiewicz, Joanna, Madry, Edyta, Musialik, Katarzyna, Walkowiak, Jaroslaw, Jakubowski, Hieronim, and Bogdański, Paweł

Subjects
OBESITY in women, PHYSICAL activity, GLOMERULAR filtration rate, KIDNEY physiology, PHYSICAL fitness, THERAPEUTICS
Abstract

Objective Obesity is associated with kidney defects. Physical activity is a key element in the treatment of obesity. The aim of this study was to compare the effect of endurance and endurance-strength training on kidney function in abdominally obese women. Methods Forty-four abdominally obese women were randomized to endurance training or endurance-strength training, three times a week for 3 months. Before and after the intervention, kidney function was assessed by measuring blood creatinine, urine creatinine, and urine albumin levels, and the albumin-to-creatinine ratio and glomerular filtration rate (GFR) were calculated. Results Renal hyperperfusion was present in both groups before the study. Following both types of physical activity, similar modifications of the investigated parameters were observed, but with no significant between-group differences. Both courses of training led to a significant increase in blood creatinine and a subsequent decrease in the GFR. A significant increase in urine creatinine and album levels, though not exceeding the range for microalbuminuria, was not accompanied by any difference in the albumin-to-creatinine ratio after endurance-strength training alone. Conclusion Three months of either endurance or endurance-strength training has a favorable and comparable effect on renal function in abdominally obese women with renal hyperfiltration. [ABSTRACT FROM AUTHOR]

Published
2016
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135. Effect of 9p21.3 Coronary Artery Disease Locus Neighboring Genes on Atherosclerosis in Mice

Author

Kim, Juyong Brian, primary, Deluna, Andres, additional, Mungrue, Imran N., additional, Vu, Christine, additional, Pouldar, Delila, additional, Civelek, Mete, additional, Orozco, Luz, additional, Wu, Judy, additional, Wang, Xuping, additional, Charugundla, Sarada, additional, Castellani, Lawrence W., additional, Rusek, Marta, additional, Jakubowski, Hieronim, additional, and Lusis, Aldons J., additional

Published
2012
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