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1,288 results on '"Jiang, Jianxin"'

125. A large-scale screen reveals genes that mediate electrotaxis in Dictyostelium discoideum

Author

Gao, Runchi, Zhao, Siwei, Jiang, Xupin, Sun, Yaohui, Zhao, Sanjun, Gao, Jing, Borleis, Jane, Willard, Stacey, Tang, Ming, Cai, Huaqing, Kamimura, Yoichiro, Huang, Yuesheng, Jiang, Jianxin, Huang, Zunxi, Mogilner, Alex, Pan, Tingrui, Devreotes, Peter N, and Zhao, Min

Subjects
Biochemistry and Cell Biology, Biological Sciences, Genetics, Underpinning research, 1.1 Normal biological development and functioning, Aetiology, 2.1 Biological and endogenous factors, Dictyostelium, Gene Knockdown Techniques, Mechanistic Target of Rapamycin Complex 2, Multiprotein Complexes, Mutation, Proto-Oncogene Proteins c-akt, Protozoan Proteins, Signal Transduction, TOR Serine-Threonine Kinases, Biochemistry and cell biology
Abstract

Directional cell migration in an electric field, a phenomenon called galvanotaxis or electrotaxis, occurs in many types of cells, and may play an important role in wound healing and development. Small extracellular electric fields can guide the migration of amoeboid cells, and we established a large-scale screening approach to search for mutants with electrotaxis phenotypes from a collection of 563 Dictyostelium discoideum strains with morphological defects. We identified 28 strains that were defective in electrotaxis and 10 strains with a slightly higher directional response. Using plasmid rescue followed by gene disruption, we identified some of the mutated genes, including some previously implicated in chemotaxis. Among these, we studied PiaA, which encodes a critical component of TORC2, a kinase protein complex that transduces changes in motility by activating the kinase PKB (also known as Akt). Furthermore, we found that electrotaxis was decreased in mutants lacking gefA, rasC, rip3, lst8, or pkbR1, genes that encode other components of the TORC2-PKB pathway. Thus, we have developed a high-throughput screening technique that will be a useful tool to elucidate the molecular mechanisms of electrotaxis.

Published
2015

127. Quantitative structural elucidation of original lignins in different varieties of waste Gleditsia sinensisLam. pods

Author

Cheng, Xichuang, Li, Pengfei, Chen, Diansong, Ma, Ling, Ji, Li, Wang, Kun, and Jiang, Jianxin

Abstract

Obtaining new materials, industrial chemicals, and energy from the conversion of agricultural and forestry biomass to fill the gap of non-renewable fossil energy has become a growing trend. As a waste biomass, the G. sinensispods (GSP) after saponin extraction are always discarded due to lack of industrial/high value-added utilization. Herein, in order to lay a foundation for the selection of pretreatment strategies of GSP and the valorization of GSP lignin, the lignins from the different varieties of GSP were isolated by double enzymatic method and quantitatively characterized. Results indicated that GSP lignin was of the GS-type (S/G, 1.1–1.4) and the interunit linkages were mainly β-O-4′ linkage, along with a certain amount of carbon-carbon linkages (≈35/Ar). L1 showed higher β-O-4′ linkage content (61.3/100 Ar) and relative molar mass (12190 mol/g). Some atypical lignin units, such as cinnamyl aldehyde end-group, cinnamyl alcohol end-groups, and ferulates, were also revealed in GSP lignins. Finally, the potential molecular structure graphs of the GSP lignin were depicted on the basis of the quantitative results. Overall, comprehensive research of the structural characteristics of GSP lignin is essential for efficiently deconstructing GSP and transforming the utilization of the cell wall components.

Published
2024
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133. A score model based on pancreatic steatosis and fibrosis and pancreatic duct diameter to predict postoperative pancreatic fistula after Pancreatoduodenectomy

Author

Guo Xingjun, Zhu Feng, Yang Meiwen, Jiang Jianxin, He Zheng, Gao Jun, Huang Tao, Zhao Rui, Zhang Leida, Wang Min, Qin Renyi, and FACS

Subjects
Pancreatic fibrosis, Pancreatic steatosis, Postoperative pancreatic fistula, Scoring model, Surgery, RD1-811
Abstract

Abstract Purposes To establish a scoring model for the risk of postoperative pancreatic fistula (POPF) following pancreatoduodenectomy (PD). Methods PD Patients from 7 institutions in 2 independent sets: developmental (n = 457) and validation cohort (n = 152) were retrospectively enrolled and analyzed. Pancreatic Fibrosis (PF) and Pancreatic Steatosis (PS) were assessed by pathological examination of the pancreatic stump. Results Stepwise univariate and multivariate analysis indicated that pancreatic duct diameter ≤ 3 mm, increased PS and decreased PF were independent risk factors for POPF and Clinically Relevant Postoperative Pancreatic Fistula (CR-POPF). Based on the relative weight and odds ratio of each factor in the POPF, a simplified scoring model was developed. And patients were stratified into high-risk group (22~28 points), medium-risk group (15~21 points) and low-risk group (8~14 points). The receiver operating characteristic curve demonstrated that the Area under the curve for the predictive model was 0.868 and 0.887 in the model design group and the external validation group. Conclusions This study establishes a simplified scoring model based on accurately and quantitatively measuring the PS, PF and pancreatic duct diameter. The scoring model accurately predicted the risk of POPF.

Published
2019
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135. Collective cell migration: Implications for wound healing and cancer invasion

Author

Li, Li, He, Yong, Zhao, Min, and Jiang, Jianxin

Subjects
Engineering, Biomedical Engineering, Cancer, Regenerative Medicine, Physical Injury - Accidents and Adverse Effects, 1.1 Normal biological development and functioning, Underpinning research, Generic health relevance, Injury (total) Accidents/Adverse Effects, Wound healing, cancer invasion, collective migration, E-cadherin, mechanical force, mechanical force Introduction, Clinical sciences, Medical biotechnology, Biomedical engineering
Abstract

During embryonic morphogenesis, wound repair and cancer invasion, cells often migrate collectively via tight cell-cell junctions, a process named collective migration. During such migration, cells move as coherent groups, large cell sheets, strands or tubes rather than individually. One unexpected finding regarding collective cell migration is that being a "multicellular structure" enables cells to better respond to chemical and physical cues, when compared with isolated cells. This is important because epithelial cells heal wounds via the migration of large sheets of cells with tight intercellular connections. Recent studies have gained some mechanistic insights that will benefit the clinical understanding of wound healing in general. In this review, we will briefly introduce the role of collective cell migration in wound healing, regeneration and cancer invasion and discuss its underlying mechanisms as well as implications for wound healing.

Published
2013

136. Cas13d wants to compete with Cas9 to benefit tumor patients by enhancing precision CAR T therapy.

Author

Ran, Hongbiao, Jiang, Jianxin, and Lin, Ping

Subjects
*CRISPRS, *TUMOR treatment, *CHIMERIC antigen receptors, *T cells, *TRANSCRIPTOMES, *CELL-mediated cytotoxicity
Abstract

The article focuses on the emergence of Cas13d as a competitive alternative to Cas9 in enhancing precision CAR T therapy for tumor patients. Topics include the development of multiplexed effector guide arrays (MEGA) using CRISPR-Cas13d, which enables precise and reversible modulation of the transcriptome in T cells, addressing challenges such as T cell exhaustion, cytotoxicity, and off-target effects associated with traditional Cas9-based approaches.

Published
2024
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