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Indonesia, as a mega biodiversity country, has various types of wild plants. Sempolan Village, part of Jember Regency, has fertile land to support the growth of a wild plant. This research investigates wild plants used as food sources by indigenous peoples in Plalangan, Sempolan Village. This study also conducts data on how wild plants were cooked, served, and enriched by nutrition notice. The method is qualitative descriptive analysis obtained from observation, interview, documentation, and literature study. So, research results found 26 species and grouped them into 19 families. In addition, the locality of these species is in yards, fields, and roadsides. The habitus identified is an herb (77%), tree (11%), shrub (8%), and liana (4%). Those foods categorize into 1) carbohydrates sources, 2) vegetables, and 3) fresh fruits. The carbohydrate sources mostly served as a side dish, compote, and flour by stewing and frying. The vegetables include 18 species served as complements of the main menu, which cooked for soup, curry, stir fry, ointment, and pothok. Fresh fruits comprise of 4 species were directly consume or make a salad. Furthermore, all food resources contain essential nutrients like vitamins, macro and micro minerals, and phytochemicals that are important for society's growth and healthy lives. Finally, Information on wild plants as a food source will increase food diversification as well as dishes.

Background : Back pain is a common discomfort in pregnancy. Back pain in pregnant women if not handled properly can lead to poor quality of life for pregnant women. So it is necessary to make efforts to overcome one of them with non-pharmacological therapy, namely pregnant yoga practice. Research Objectives: To determine the effect of pregnant yoga on reducing back pain in pregnant women in the second and third trimesters in the Kasreman Health Center Work Area, Ngawi Regency, East Java. Research Methods: This type of research is Quasi Experiment with (Pretest and post test without control group design). The population in this study was pregnant women in the second and third trimesters in the Kasreman Health Center Work Area, Ngawi Regency, East Java. A sample of 30 people was taken by purposive sampling technique. The dependent variable is pregnant yoga and the independent variable is the decrease in back pain for pregnant women in the second and third trimesters. The research instrument used NRS (Numerical Rating Scale). Data processing using editing, coding, tabulating with Wilcoxon test data analysis. Research Results: The results showed that back pain before doing yoga during pregnancy, most of the respondents experienced back pain with a minimum distribution scale of 1 (mild pain) category of pain scale as many as 5 respondents and a maximum distribution of scale with a pain scale category of 8 (severe pain) as many as 1 respondent, while After being given yoga, most of the pregnant women became the minimum distribution scale with a pain scale category of 0 (Mother Doesn't Feel Pain) as many as 9 respondents and a distribution scale that was maximum with a pain scale category of 5 (Medium Pain) as many as 2 respondents. The Wilcoxon statistic test showed that p value= 0.000 < (0.05) so that H1 is accepted. Conclusion: There is an effect of yoga on reducing back pain in pregnant women in the second and third trimesters in the Kasreman Health Center Work Area, Ngawi Regency, East Java. Key Word : Pregnancy Yoga, Back Pain in Second and Third Trimester

Background: Lower back pain is discomfort under costa and top of the gluteal inferior that experienced by 70% pregnant women in Indonesia. Warm compresses increase oxygenation and acupressure provides balance to the body. Aim this study is to analyze the effect of warm compresses and acupressure on lower back pain in third trimester pregnant women. Methods: This research uses pre-experimental design with pretest-posttest group design. It is used Purposive Sampling with 30 third trimester of pregnant woman as respondent. Bivariate analysis use the shapiro wilk by comparing pre and posttests using paired t test. Meanwhile, to compare the effect of warm compresses and acupressure by using independent t test.Results: Bivariate analysis showed that p value of warm compresses is 0.00 with the difference of mean is 2,3. While, p value of acupressure is 0.00, with the difference of mean is 1,2. Conclusion: There is an effect of warm compresses and acupressure on lower back pain in the third trisemeter of pregnant women.

The meats physical quality is reflected by meat characteristics and reference for consumers to choose good quality of beef. Several factors that affect the meat quality are interaction genetic and environmental factors during maintenance until post slaughter. The research was conducted to evaluate the meat physical quality with the breeds factor (Red Brahman Crossbred and Droughtmaster), genders (steer and heifer), and ages (PI0, PI2, and PI4). The research materials were 120 samples of Longissimus dorsi ribs 12th from AM FARM abattoir and tested physical quality meat on QC Laboratory of KASA Company. The data were analyzed by ANOVA and Least Significant Difference if there were any significant. The variables observed were pH, meat color, WHC, cooking loss, and tenderness. The results showed that breeds factors have no significant difference on meat physical quality. The factor of genders nested to breeds has very significance (P

Increased reproduction is closely related to increased bull productivity. The high productivity of these bulls will increase the amount of straw. The productivity capability of Bali bull is essential in producing straw. High productivity will result in high straw production as well. So the research aims to determine the productivity of Bali bull at SNAIC in the last year. 31 Bali bulls aged 4 – 11 years were used in this study at SNAIC. Total 1.273 ejaculations collection from these during one year. The parameters used in this study include the number of successful holding of each bull, total productivity, and estimation of straw production in one year. From 31 Bali Bulls, the ranking was grouped into two based on values of sperm productivity. The two groups were analyzed using an unpaired T-test. The results showed that the pH, volume, individual motility, concentration, and abnormality were significantly different (P < 0.05) between the high and low groups. The percentage of successful shelters, total TSM, and the number of straws produced in one year in the high productivity group were higher than the low productivity group. High productivity groups are the best with high productivity potential and potential as a source of semen.

Madura cattle is local cattle in Indonesia. This cattle hasphenotypic variations in growth traits. This study aimed to identifyWe recorded body weight (BW) and body measurements of 51 Madura cattle aged 1-4 years in Waru District, Madura. The LEP gene genotyping was conducted using the polymerase chain reaction-restricted fragment length polymorphism method withA 267 bp DNA fragment of the LEP gene was successfully amplified using a pair of primers. This study revealed three genotypes (TT, TC, and CC) and two alleles (T and C). The frequencies of TT, TC, and CC genotypes were 0.275, 0.45, and 0.275, respectively, whereas the frequencies of T and C alleles were 0.500 and 0.500, respectively. The c.73TC mutation was significantly associated with BW, body length (BL), and chest girth (CG) (pConclusively, LEP gene polymorphism (c.73TC) exon 2 was polymorphic and associated with body measurements, especially BW, BL, and CG.

This study aims to identify the business model canvas (BMC) and analyze the implications of the cost structure of raising horses in Rumah Berkuda Yogyakarta which has a concept horse riding school. The research method uses descriptive qualitative and quantitative. The results showed that the targeted customer segments were parents of children, adults, photo shoots with horses and horses’ owner without stable. Value Propositions in the form of strategic locations, riding training for children and adults, stable rental services, various training package prices, training time to follow visitors, and horses for training more than 2 tails. Channels are in the form of word of mouth and social media promotions. Customer relationships in the form of making t-shirts, renting cages and providing souvenirs and bonus lessons. Revenue stream from equestrian training services and cage rental. Key resources in the form of horses, human resources, facilities, and finance. Key activities include horse maintenance, visitor services as well as promotion and administration. Kay partnership in the form of shops for feed, medicine and equestrian equipment, providers of cage mats, providers of grass, t-shirt convection and social media around. The cost structure includes the basic costs of maintenance and operational costs of horse maintenance. The total monthly percentage of fixed cost is 19.44% and the percentage of total variable cost is 80.55% of the total percentage of horse maintenance.

Sexing spermatozoa bertujuan untuk mengatur jenis kelamin sesuai harapan. Salah satu metode sexing adalah dengan menggunakan Sentrifugasi Gradien Densitas Percoll. Tujuan dari penelitian ini adalah untuk mengetahui berbagai parameter motilitas menggunakan Computer-assisted Sperm Analysis dan proporsi spermatozoa X dan Y menggunakan metode Sentrifugasi Gradien Densitas Percoll dengan volume awal yang berbeda. Penelitian dilakukan di Loka Penelitian Sapi Potong Grati, Pasuruan, pada bulan Januari sampai Maret 2020. Materi yang digunakan adalah Semen Sapi Peranakan Ongole berumur ±5 tahun dan bobot badan ±700 kg sebanyak 3 ekor, motilitas masa ≥ 2+ dan motilitas individu ≥ 70%. Metode yang digunakan adalah eksperimental menggunakan Rancangan Acak Kelompok (RAK) dengan 3 perlakuan volume saat sexing yaitu P1= 1 ml, P2= 1,5 ml dan P3= 2 ml dengan ulangan 11 kali. Ulangan juga berfungsi sebagai kelompok (block). Hasil penelitian menunjukkan bahwa setelah dilakukan sexing dan pendinginan hingga 5oC persentase motilitas adalah P1:71,02+10,08 %; P2: 79,63+8,65 % dan P3: 83,38+6,67 %, sedangkan motilitas progresif pada P1: 47,68+8,71%; P2: 59,61+7,26 % dan P3: 62,21+6,74%. Curvilinear Velocity (VCL) pada P1: 50,9±7,73 µm/s, P2: 55,2±5,03 µm/s dan P3: 53,2±5,97%. Straight-line Velocity (VSL) pada P1: 20,8±8,19 µm/s; P2: 21,6±6,02 µm/s; P3: 22,1±5,77 µm/s, sedangkan Average Path Velocity (VAP) pada P1: 30,2±7,82 µm/s; P2: 32,5±6,14 µm/s dan P3 : 31,5±6,18 µm/s. Linearity (LIN) pada P1: 40,2±12,26%;P2: 39,1±10,31%;P3: 39,8±7,25%. Straightness (STR) pada P1: 67,2±11,20%; P2: 65,7±10,06%;P3: 67,2±7,92% sedangkan Wobble (WOB) pada P1: 59±9,49%; P2: 58,8±8,63% dan P3: 59,1±7,74%. Kesimpulan dari penelitian adalah motilitas dan motilitas progresif spermatozoa pada sampel dengan volume awal 2 ml lebih baik dibandingkan dengan sampel dengan volume awal 1 ml dan 1,5 ml. (Characteristics of Sexing Spermatozoa Motility in Ongole Cattle with Different Initial Volume) ABSTRACT. Sperm sexing is a technique of sorting a specific type of sperm cell to fertilize the egg cell. One of the sexing methods that can be used for spermatozoa sexing is percoll gradient density centrifugation. This research aims to find out various motility parameters using Computer-assisted Sperm Analysis and the proportion of spermatozoa X and Y using density gradient centrifugation method percoll with different initial volume. The study was conducted at Grati Beef Cattle Research Station, Pasuruan, from January to March 2020. Materials of the study were Semen of 3 Filial Ongole Cattles, aged ± 5 years, with ± 700 kg body weight, mass motility ≥ 2+, and individual motility ≥ 70%. The method used was experimental using a Randomized Group Design with 3 treatment volume when sexing i.e. T0= 1 ml, T1= 1.5 ml dan T2= 2 ml with repetition 11 times. The repetition also functions as groups (blocks). The results showed that after sexing and cooling up to 5oC, the percentage of motility is T0:71.02+10.08 %; T1: 79.63+8,65 % and T2: 83.38+6.67 %. Progressive motility on T0: 47.68+8.71%; T1: 59.61+7.26 % and T2: 62.21+6.74%. Curvilinear Velocity (VCL) on T0: 50.9±7.73 µm/s, T1: 55.2±5.03 µm/s and T2: 53.2±5.97%. Straight-line Velocity (VSL) on T0: 20.8±8.19 µm/s; T1: 21.6±6.02 µm/s; T2: 22.1±5.77 µm/s. The Average Path Velocity (VAP) on T0: 30.2±7.82 µm/s; T1: 32.5±6.14 µm/s and T2 : 31.5±6.18 µm/s. Linearity (LIN) on T0: 40.2±12.26%; T1: 39.1±10.31%;T2: 39.8±7.25%. Straightness (STR) on T0: 67.2±11.20%; T1: 65.7±10.06%; T2: 67.2±7.92%. Wobble (WOB) on T0: 59±9.49%; T1: 58.8±8.63% and T2: 59.1±7.74%. The conclusion of the study is the motility and progressive motility of spermatozoa in samples with an initial volume of 2 ml is better than samples with an initial volume of 1 ml and 1.5 ml.

Inseminasi buatan dengan menggunakan semen sexing diharapkan menghasilkan pedet dengan jenis kelamin sesuai harapan. Tujuan dari penelitian ini adalah untuk mengetahui kualitas, proporsi, dan jumlah produksi straw sexing menggunakan metode sentrifugasi gradien densitas percoll dengan volume awal semen yang berbeda. Penelitian dilakukan di Loka Penelitian Sapi Potong, Kecamatan Grati, Kabupaten Pasuruan dan Laboratorium Reproduksi Ternak, Fakultas Peternakan Universitas Brawijaya, Malang. Materi yang digunakan adalah semen sapi peranakan ongole berumur berkisar lima tahun dan bobot badan berkisar 700 kg sebanyak tiga ekor, motilitas massa ≥2+ dan motilitas individu ≥70%. Metode yang digunakan adalah eksperimental dengan tiga perlakuan volume awal saat sexing, yaitu 1 (P1); 1,5 (P2); dan 2 (P3) ml dengan ulangan 11 kali (ulangan berfungsi sebagai kelompok). Data dianalisa menggunakan Rancangan Acak Kelompok (RAK). Hasil penelitian menunjukkan bahwa perbedaan volume awal semen tidak berpengaruh (menurun) terhadap motilitas, viabilitas, abnormalitas, konsentrasi, total spermatozoa motil, recovery rate dan proporsi spermatozoa (P>0,05). Pengaruh yang sangat nyata (meningkat) terhadap jumlah produksi straw (P0.05). On the other hand, the difference in the initial volume of semen had a very significant effect (increased) on the amount of frozen semen production (P

Penelitian ini bertujuan untuk mengetahui pengaruh pengencer CEP-3 kuning telur terhadap kualitas semen cair sapi Peranakan Ongole (PO) pada media simpan yang berbeda. Penelitian dilaksanakan di Laboratorium Lapang Fakultas Peternakan Universitas Brawijaya pada bulan Agustus sampai September 2017. Materi penelitian yang digunakan yaitu semen segar sapi Peranakan Ongole (PO) berumur 2 tahun ditampung menggunakan vagina buatan. Metode penelitian yang digunakan adalah eksperimental laboratorium dengan 5 perlakuan yaitu P0 (media simpan refrigerator suhu 5 oC), P1 (media simpan termos kosong suhu 28 oC), P2 (media simpan termos berisi es batu suhu 0 oC), P3 (media simpan termos berisi air es suhu 9 oC), dan P4 (media simpan termos berisi air sumur suhu 25 oC) masing-masing perlakuan menggunakan 10 ulangan apabila terdapat perbedaan yang nyata dilanjutkan dengan uji Jarak Duncan. Hasil analisis ragam pada penyimpanan hingga jam ke-8 menunjukkan berbeda sangat nyata (P 70 % dikemas didalam dan tanpa straw.

Pejantan untuk program inseminasi buatan adalah pejantan unggul yang diseleksi berdasarkan libido dan produksi spermatozoa. Penelitian bertujuan untuk menganalisa hubungan antara tingkah laku seksual dengan produksi spermatozoa sapi Brahman. Materi penelitian adalah 3 pejantan sapi Brahman yang ditampung menggunakan vagina buatan. Metode penelitian adalah observasional dengan mengamati waktu reaksi dan lama ejakulasi. Observasi laboratorium dengan mengamati volume, konsentrasi, dan motilitas individu untuk menghitung total spermatozoa (TS) dan total spermatozoa motil (TSM). Pengamatan dilakukan sebanyak 10 ulangan. Data dianalisa secara deskriptif serta hubungan antara tingkah laku seksual dan produksi spermatozoa dihitung dengan korelasi Pearson . Hasil penelitian menunjukkan terdapat variasi tingkah laku seksual dan produksi spermatozoa pada sapi Brahman. Individu Cipta mempunyai waktu reaksi dan lama ejakulasi paling singkat serta produksi spermatozoa paling rendah. Hubungan signifikan antara waktu reaksi dengan TS serta TSM pada individu Bizzyard dengan koefisien determinasi (R 2 ) sebesar 14 % dan 20%. Hubungan sangat signifikan antara lama ejakulasi dengan TS serta TSM pada individu Cipta dengan R 2 sebesar 46% dan 58%. Kesimpulan penelitian adalah terdapat hubungan antara tingkah laku seksual dengan produksi spermatozoa pada sapi Brahman, dengan koefisien korelasi (r) tertinggi sebesar 0,72 dan R 2 sebesar 58% antara lama ejakulasi dan total spermatozoa motil pada individu Cipta. (The relationship between sexual behavior and semen production in Brahman bulls) ABSTRACT. Bulls for artificial insemination must be selected based on libido and sperm production. The study aimed to analyze the relationship between sexual behavior with sperm production on Brahman bulls. The material was 3 Brahman bulls collected using artificial vagina. The method was observational by observing reaction time and ejaculation duration. Laboratory observations by observing volume, concentration, and motility to calculate total sperm (TS) and total motile sperm counts (TMSC). Observations were replicated 10 times. The data were analyzed descriptively, the relationship between behavior and sperm production was examined using Pearson correlation. The results showed that there were variations in sexual behavior and sperm production in each bull. Cipta have the shortest reaction time and ejaculation duration and the lowest sperm production. A significant relationship between reaction time and TS and TMSC in Bizzyard with coefficient of determination (R 2 ) of 14% and 20%. A very significant between ejaculation duration with TS and TMSC in Cipta with R 2 of 46% and 58%. In conclusion, there was a relationship between sexual behavior and sperm production in Brahman bulls, with the highest correlation coefficient (r) of 0.72 and R 2 of 58% between ejaculation duration and the total motile sperm counts in Cipta.

The purpose of this research was to investigate the successful difference of artificial insemination (AI) using frozen semen and liquid semen with CEP-3 + egg yolk diluent of Ongole Crossbred cow indicated by Non Return Rate (NRR) and Conception Rate (CR). The materials used were 54 heads of cow which selected purposively using some criteria such as healthy, free from any reproduction disorder and having at least sexual desire criteria 3A (red, swollen and warm). This field experiment consisted of direct observation and interview to acquire primary and secondary data. The datas were analyzed descriptively and continued by Chi-square test. The results showed that the value of NRR was insignificant influenced between the two treatment. The Conception Rate for frozen semen and liquid semen was found 62.96% and 51.85% respectively. The conclusion of this experiment was the successful rate of artificial insemination (AI) using liquid semen with the CEP-3 + 20% egg yolk diluent was lower than artificial insemination using frozen semen. The value of Conception Rate of frozen semen treatment was 62.96% and the liquid semen treatment was 51.85%.

Tumor suppressor gene p53 is one of the specific parameters of the occurrence of brain neuron death in animal models of brain ischemia. Several studies show that the duration of reperfusion of tBCCAO has an effect on the expression of p53 protein. The duration of ischemia for 5 minutes followed by reperfusion of 1 hour, 4 hours, 8 hours, 24 hours, and 7 days increased the expression of p53 protein. 30-minute ischemia induction followed by 4-hour reperfusion showed increased p53 protein expression in rat serum. It is not yet known how the influence of tBCCAO ischemia duration on p53 expression in rat brain. This study used Wistar, male rats, aged 3-4 months, weighing 175-250 gr, and healthy. Group A was a group of tBCCAO rats with a duration of 5 minutes ischemia, 24-hour reperfusion duration (five). Group B was a group of tBCCAO mice with a duration of 10 minutes ischemia, 24-hour reperfusion (five). Group C is a group of sham operated mice (five). The expression of p53 protein is semi quantification of p53 expressed on pyramidal neuron of the frontal brain (Cortex Prefrontalis, striatum) and CA1 hippocampus, with IHC staining using anti-p53 antibodies. P53 expression will be seen in the cytoplasm of pyramidal neuron CA1 hippocampus. Cytoplasm of neurons will be brownish in color. The semi-quantification method of p53 expression uses the ALLRED score. Data analysis using one way ANOVA test. The analysis found differences in frontal brain p53 expression (cortex prefrontalis and striatum) (p = 0.00) and there were differences in p53 expression in pyramidal neuron CA1 hippocampal (p = 0.013). There was an effect of the duration of tBCCAO on expression of p53 protein in rat brain after 24-hour reperfusion. Keywords: tBCCAO’s duration, p53, rat’s brain

BackgroundSodium nitrite induces hypoxia and oxidative stress in the hippocampus, decreasing the number of neurons in the hippocampus and cognitive function. Propolis contains chrysin that has antioxidant effects that are expected to inhibit neuronal damage in the hippocampus. This study aims to determine the effects of propolis on the expression Bcl-2-associated X protein (Bax) and the number of neurons in the rat hippocampus receiving sodium nitrite.MethodsThis study of laboratory experimental design was conducted on 18 male Wistar strain rats (Rattus norvegicus), they were randomized into 3 groups: one control group (K) received sodium nitrite and two intervention groups (P1 and P2) received sodium nitrite and propolis at doses of 100 and 200 mg/kgBW. Treatment with sodium nitrite and propolis were given for 60 days, followed by termination. The number of neurons and Bax expression in the hippocampal CA1 area were measured. One-way ANOVA was used to analyze the data.ResultsThere were significant differences in Bax expression between group K and groups P1 and P2 (p

Tujuan dari penelitian ini untuk mengetahui tingkat keberhasilan inseminasi buatan double dosis pada jam ke-8 dan jam ke-16 pada sapi Persilangan Ongole dengan kualitas berahi yang berbeda. Materi dalam penelitian ini menggunakan 25 ekor sapi betina Persilangan Ongole yang dipilih secara purposive. Metode dalam penelitian ini adalah observasi langsung di lapang, dengan menyeleksi sapi betina dengan kriteria BCS >3, umur >1,5 tahun dan telah melahirkan. Penelitian ini menggunakan semen beku pejantan Limousin yang diproduksi oleh Balai Besar Inseminasi Buatan (BBIB) Singosari, Malang. Hasil penelitian menujukkan persentase karakteristik yaitu Non Return Rate (NRR-1, NRR-2) masing-masing 92,31% dan 58,33%, Conception Rate (CR) 38,46% dan 25%, Pregnancy Rate (PR) 53,85% dan 50%. Suhu Vagina 37,0-37,9ºC dan >38,0ºC NRR-1, NRR-2 masing-masing 77,78% dan 57,14%, CR 14,28% dan 33,33%, PR 42,86% dan 55,56%. Lendir servik (ada, basah, sedikit) dan (ada, basah, banyak) NRR-1, NRR-2 masing-masing 89,47% dan 16,67%, CR 31,58% dan 16,67%, PR 47,37% dan 66,67%. Lendir Servik pH 7 dan pH 8 NRR-1, NRR-2 masing-masing 77,78% dan 68,75%, CR 11,11% dan 37,50%, PR 44,44% dan 56,25%. Nilai HD 21-30 dan 31-40 NRR-1, NRR-2 masing-masing 50% dan 82,35%, CR 12,50% dan 35,29%, PR 50% dan 52,94%. Kesimpulan penelitian ini bahwa karakteristik warna vulva merah, suhu vagina >38ºC, karakteristik lendir (ada, basah, banyak), lendir servik pH 8 dan nilai HD 31-40, memberikan persentase kebuntingan yang lebih tinggi.

The purposes of this study were determine the carcass characteristics and physical meat quality of Brahman Cross Steer on different rest periods. The experiment was conducted on June 2015 at Slaughter house Cianjur Arta Makmur Ltd. in the Cianjur District, West Java. The total number of cattle used in the study was 30 heads of Brahman Cross Steer treated for 18 hours lairage duration and 30 heads of Brahman Cross Steer treated for 3 hours lairage duration from Pasir Tengah feedloter Ltd (Holding Widodo Makmur Perkasa Ltd). The method used a case study. Data were analyzed using unpaired t test. The results showed that the carcass characteristics including slaughtered weight, hot carcass weight, chilled carcass weight and carcass weight loss percentage were not significantly different, while the dressing percentage for 18 hours lairage duration (54.16±1.62%) and 3 hours lairage duration (55.60±2.00%) was significantly different (P

Introduction: Stress cause changes in the structure and function of the prefrontal cortex. Chronic restraint stress causes the shortening of dendrites in the medial prefrontal cortex. Centella asiatica is an herb that has antioxidant and neuroprotective effects. The purpose of this study is to understand the effect of Centella asiatica on the active protein caspase-3 apoptosis executor expression in the prefrontal cortex. Methods: This study was a laboratory experimental using posttest only control group design. The study used 25 pieces of stored biological material sample in the form of rat brain tissue paraffin blocks. These brain tissues came from 25 rats that were divided into groups of P1, P2, P3, P4 and P5. P1 group treated with restraint stress. Group P2, P3 and P4 were subjected to restraint stress and Centella asiatica ethanolic extract with therapeutic dose of 150, 300 and 600 mg/kg/day. P5 group received stress treatment and fluoxetine with therapeutic dose of 10 mg/kg/day. On the 23rd day, termination, perfusion and brain tissues sampling were executed. Furthermore, the brain tissues were made into paraffin blocks, cut into specimen, immunohistochemical stained, and count the cells which were expressing active caspase-3. Results: There were significant differences in the expression of active caspase-3 between P1 to P2, P3, P4 and P5 groups (p = 0.00). There were no significant differences between P2, P3, P4 and P5 groups. Conclusion: Centella asiatica ethanolic extract can inhibit active caspase-3 expression in the prefrontal cortex of Sprague Dawley rats that were induced with chronic restraint stress.

Totok Payudara, Oxytocin Massage And Colostrum . The purpose of this study was to determine differences in time of leaking colostrums in mothers post partum in doing oxytocin massage with totok payudara . This study design used is quasi-experimental design with the design Nonequivalent Control Group Design . Reserch conducted at the midwives (BPM) at health centers working area Klaten who were divided into 2 groups, i.e an experimental group consisting of 30 respondens to whom oxytocin massage was given and a control group consisting of 30 responden to whom totok payudara was given . Bivariate data analysis using statistical test used is t –test. Results of the study showed the average time of leaking colostrums in experimental and control groups were 152,67 minutes and 137,23 minutes. The result of hypothesis test using T-test obtained the value of p= 0.668 (p>0.05). Conclusion: there is no difference time between oxytocin massage and totok payudara to the leaking colostrum Key Word: Totok Payudara, Oxytocin Massage And Colostrum LINK OJS 3 ARTIKEL "PENINGKATAN KECEPATAN PENGELUARAN KOLOSTRUM DENGAN PERAWATAN TOTOK PAYUDARA DAN PIJAT OKSITOSIN PADA IBU POST PARTUM"

Class Pregnant Women ; Levels Of Anxiety . Anxiety is one of the main factors that influence whether or not an action smoothness of delivery. Pregnant women with sufficient knowledge proved to have a low risk of the occurrence of complications of childbirth. Educational intervention in the form of counseling mothers on maternal class. Purpose is to k nowing the differences in levels of maternal anxiety on the class of pregnant woman in the Puskesmas Tulung. Type of quasi-experimental research design with a static group comparison. The population is pregnant women PHC Tulung region. Samples are 36 pregnant women who meet the criteria cadre, which consisted of 18 pregnant women who attend classes pregnant women and 18 pregnant women who do not attend classes pregnant women with purposive sampling technique. Respondents who attend classes maternal anxiety has a value of at least 6, the maximum value of 20 with an average of 15.4 and the majority of respondents (61.1%) had anxiety medium category. Those who do not attend classes anxiety mother has a value of at least 14, the maximum value of 27, with an average of 20.67 and a majority of respondents have anxiety was (88.9%). There are differences in the level of maternal anxiety that follows and that pregnant women do not attend classes in the Puskesmas Tulung, with significant differences in anxiety score (p value = 0.000). Keywords : Class Pregnant Women, Levels Of Anxiety Link OJS 3 artikel PERBEDAAN TINGKAT KECEMASAN IBU BERSALIN PADA KELAS IBU HAMIL DI WILAYAH KERJA PUSKESMAS TULUNG

The purpose of this study was to determine the success of artificial insemination (AI) using liquid sperm Filial Ongole Bulls after sexed by the percoll density gradient centrifugation (PDGC) and egg white sedimentation (EWS) sexed methods. The study materials were 135 cattle were inseminated using non sexed semen, PDGC and EWS sexed methods. Non-return rate (NRR) and the conception rate (CR) are observed. The research design used for the success of AI using chi square. The result of pregnancy examination at the age of 3 months found 21 head of pregnant cows from 45 cattle in AI with without sexed (control), 14 head of pregnant cattle from 45 cattle in the AI with EWS method, 18 head of pregnant cattle from 45 cattle in AI with PDGC method. The result showed that CR value using liquid sperm result of EWS, PDGC and without sexed were very significant (P

Latar Belakang : Menstruasi merupakan tanda pubertas seorang wanita dan menjadi rutinitas wanita yang masih dalam masa subur. Siklus ini menimbulkan ketidaknyamanan, salah satunya nyeri menstruasi. Nyeri ini dapat berupa kram ringan hingga dapat mengganggu kegiatan sehari-hari. Nyeri menstruasi dapat dikurangi secara farmakologis dan non farmakologis. Secara non farmakologis salah satunya adalah dengan akupresure pada titik Hegu. Tujuan : Penelitian ini bertujuan untuk mengetahui pengaruh akupresure titik hegu terhadap nyeri menstruasi. Metode : Penelitian ini merupakan penelitian observasional. Jenis penelitian quasy eksperiment dengan rancangan non equivalent control group. Populasi aktualnya yaitu santriwati kelas XI Madrasah Bertaraf Internasional Amanatul Ummah Mojokerto yang mengalami nyeri menstruasi sebanyak 126 orang. Teknik pengambilan sample adalah purposive sampling dengan perhitungan rumus Slovin, didapatkan sebanyak 56 responden. Instrumen penelitian menggunakan lembar observasi dengan pengukuran Numeric Rating Scale. Analisis data menggunakan independent t-test. Hasil : Hasil penelitian menunjukkan bahwa terdapat perbedaan penurunan nyeri menstruasi antara kelompok eksperimen dan kelompok kontrol dengan p=0,001 (p

Malaria is a serious public health problem in Indonesia. This disease is one of several indicators that have been targeted by the Millennium Development Goals (MDGs) 2015 to become Sustainable Development Goals (SDGs). In malaria-endemic areas such as in Kokap, Kulon Progo, Yogyakarta, reported malaria cases declined in 2000 with no case, but increased in 2011 following by some outbreaks in 2012. Future outbreaks could be avoided when health personnel including Primary Health Care (PHC) microscopists and entomologist surveyors were equipped with adequate trainings related to malaria. This training also be used for refreshment for existing personnel to support elimination at the Kokap I, Kokap II, and several nearby PHCs. In October 2013, the Department of Parasitology, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada carried out microscopic training and basic entomology related to malaria in the working area of PHC in Kokap. Training methods were giving material and direct activities in the field. The training provided basic entomology training, malaria microscopic training and malaria vector mapping using a Global Positioning System (GPS). The training facilitated PHC personnel to do correct identification of Plasmodium species, identification of old and new vector breeding sites, and found out how to map vector distribution and malaria cases in their area. The results showed that there was an increase in knowledge from PHC personnel who participated in the pre-test and post-test. From field activity, three adult mosquitoes species were found i.e. Anopheles vagus, An. annularis and An. maculatus. Nulliparous and parous mosquitoes from adult mosquito based on the examination of their tracheole skeins of ovarium were found from the collected mosquitoes although oocyst in midgut and sporozoite in salivary gland were not found during adult mosquito surgical. Similar trainings were expected to be applied in other similar areas in the future.