117 results on '"LADENHEIM, BRUCE"'
Search Results
102. Methamphetamine-induced serotonin neurotoxicity is mediated by superoxide radicals
- Author
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Hirata, Hiroshi, primary, Ladenheim, Bruce, additional, Rothman, Richard B., additional, Epstein, Charles, additional, and Cadet, Jean Lud, additional
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- 1995
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103. CuZn-superoxide dismutase (CuZnSOD) transgenic mice show resistance to the lethal effects of methylenedioxyamphetamine (MDA) and of methylenedioxymethamphetamine (MDMA)
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Cadet, Jean Lud, primary, Ladenheim, Bruce, additional, Baum, Ira, additional, Carlson, Elaine, additional, and Epstein, Charles, additional
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- 1994
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104. (±)-3,4-Methylenedioxymethamphefamine and Metabolite Disposition in Plasma and Striatum of Wild-Type and Multidrug Resistance Protein 1a Knock-Out Mice.
- Author
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Scheidweiler, Karl B., Ladenheim, Bruce, Barnes, Allan J., Cadet, Jean Lud, and Huestis, Marilyn A.
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ECSTASY (Drug) , *PHARMACOKINETICS , *MULTIDRUG resistance , *NEUROTOXICOLOGY , *LABORATORY mice , *ANIMAL models in research , *GAS chromatography , *MASS spectrometry - Abstract
The article presents a study that characterizes methylenedioxymethamphetamine (MDMA) pharmacokinetics in murine plasma and bran to determine whether mdr1a alters MDMA distribution. It notes that mice having inadequate multidrug resistance protein mdr1a has been protected from MDMA-induced neurotoxicity, highlighting a mechanism. Moreover, plasma and brain specimens from knock-out mice have been collected after MDMA to quantify the distribution through gas chromatography-mass spectrometry.
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- 2011
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105. Monoamine Oxidases Regulate Telencephalic Neural Progenitors in Late Embryonic and Early Postnatal Development.
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Aiwu Cheng, Scott, Anna L., Ladenheim, Bruce, Chen, Kevin, Xin Ouyang, Lathia, Justin D., Mughal, Mohamed, Cadet, Jean Lud, Mattson, Mark P., and Shih, Jean C.
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NEUROTRANSMITTERS ,BRAIN ,NEURAL stem cells ,TELENCEPHALON ,MITOSIS ,SEROTONIN - Abstract
Monoamine neurotransmitters play major roles in regulating a range of brain functions in adults and increasing evidence suggests roles for monoamines in brain development. Here we show that mice lacking the monoamine metabolic enzymes MAO A and MAO B (MAO AB-deficient mice) exhibit diminished proliferation of neural stem cells (NSC) in the developing telencephalon beginning in late gestation [embryonic day (E) 17.5], a deficit that persists in neonatal and adult mice. These mice showed significantly increased monoamine levels and anxiety-like behaviors as adults. Assessments of markers of intermediate progenitor cells (IPC) and mitosis showed that NSC in the subventricular zone (SVZ), but not in the ventricular zone, are reduced in MAO AB-deficient mice. A developmental time course of monoamines in frontal cortical tissues revealed increased serotonin levels as early as E14.5, and a further large increase was found between E17.5 and postnatal day 2. Administration of an inhibitor of serotonin synthesis (parachlorophenylalanine) between E14.5 and E19.5 restored the IPC numbers and SVZ thickness, suggesting the role of serotonin in the suppression of IPC proliferation. Studies of neurosphere cultures prepared from the telencephalon at different embryonic and postnatal ages showed that serotonin stimulates proliferation in wild-type, but not in MAO AB-deficient, NSC. Together, these results suggest that a MAO-dependent long-lasting alteration in the proliferation capacity of NSC occurs late in embryonic development and is mediated by serotonin. Our findings reveal novel roles for MAOs and serotonin in the regulation of IPC proliferation in the developing brain. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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106. Dietary restriction mitigates cocaine-induced alterations of olfactory bulb cellular plasticity and gene expression, and behavior.
- Author
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Xiangru Xu, Mughal, Mohamed R., Scott Hall, F., Perona, Maria T. G., Pistell, Paul J., Lathia, Justin D., Chigurupati, Srinivasulu, Becker, Kevin G., Ladenheim, Bruce, Niklason, Laura E., Uhl, George R., Cadet, Jean Lud, and Mattson, Mark P.
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GENE expression ,OLFACTORY nerve ,COCAINE ,MICE ,DOPAMINE ,ENERGY metabolism ,ANIMAL nutrition - Abstract
J. Neurochem. (2010) 114, 323–334. Because the olfactory system plays a major role in food consumption, and because ‘food addiction’ and associated morbidities have reached epidemic proportions, we tested the hypothesis that dietary energy restriction can modify adverse effects of cocaine on behavior and olfactory cellular and molecular plasticity. Mice maintained on an alternate day fasting (ADF) diet exhibited increased baseline locomotion and increased cocaine-sensitized locomotion during cocaine conditioning, despite no change in cocaine conditioned place preference, compared with mice fed ad libitum. Levels of dopamine and its metabolites in the olfactory bulb (OB) were suppressed in mice on the ADF diet compared with mice on the control diet, independent of acute or chronic cocaine treatment. The expression of several enzymes involved in dopamine metabolism including tyrosine hydroxylase, monoamine oxidases A and B, and catechol-O-methyltransferase were significantly reduced in OBs of mice on the ADF diet. Both acute and chronic administration of cocaine suppressed the production of new OB cells, and this effect of cocaine was attenuated in mice on the ADF diet. Cocaine administration to mice on the control diet resulted in up-regulation of OB genes involved in mitochondrial energy metabolism, synaptic plasticity, cellular stress responses, and calcium- and cAMP-mediated signaling, whereas multiple olfactory receptor genes were down-regulated by cocaine treatment. ADF abolished many of the effects of cocaine on OB gene expression. Our findings reveal that dietary energy intake modifies the neural substrates underlying some of the behavioral and physiological responses to repeated cocaine treatment, and also suggest novel roles for the olfactory system in addiction. The data further suggest that modification of dietary energy intake could provide a novel potential approach to addiction treatments. [ABSTRACT FROM AUTHOR]
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- 2010
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107. Delta opioid peptide [D-Ala2, D-Leu5]enkephalin causes a near complete blockade of the neuronal damage induced by a single high dose of methamphetamine: Examining the role of p53.
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Hayashi, Teruo, Hirata, Hiroshi, Asanuma, Masato, Ladenheim, Bruce, Tsao, Li-I, Cadet, Jean Lud, and Su, Tsung-Ping
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- 2001
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108. Methamphetamine Preconditioning Causes Differential Changes in Striatal Transcriptional Responses to Large Doses of the Drug.
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Cadet, Jean Lud, Brannock, Christie, Ladenheim, Bruce, McCoy, Michael T., Beauvais, Genevieve, Hodges, Amber B., Lehrmann, Elin, Wood, William H., Becker, Kevin G., and Krasnova, Irina N.
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METHAMPHETAMINE , *DRUG toxicity , *DRUG abuse , *POLYMERASE chain reaction , *HEAT shock proteins , *GENETIC transcription , *GENE expression , *LABORATORY rats - Abstract
Methamphetamine (METH) is a toxic drug of abuse, which can cause significant decreases in the levels of monoamines in various brain regions. However, animals treated with progressively increasing doses of METH over several weeks are protected against the toxic effects of the drug. In the present study, we tested the possibility that this pattern of METH injections might be associated with transcriptional changes in the rat striatum, an area of the brain which is known to be very sensitive to METH toxicity and which is protected by METH preconditioning. We found that the presence and absence of preconditioning followed by injection of large doses of METH caused differential expression in different sets of striatal genes. Quantitative PCR confirmed METH-induced changes in some genes of interest. These include small heat shock 27 kD proteins 1 and 2 (HspB1 and HspB2), brain derived neurotrophic factor (BDNF), and heme oxygenase-1 (Hmox-1). Our observations are consistent with previous studies which have reported that ischemic or pharmacological preconditioning can cause reprogramming of gene expression after lethal ischemic insults. These studies add to the growing literature on the effects of preconditioning on the brain transcriptome. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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109. Incubation of methamphetamine craving in punishment-resistant individuals is associated with activation of specific gene networks in the rat dorsal striatum.
- Author
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Daiwile AP, McCoy MT, Ladenheim B, Subramaniam J, and Cadet JL
- Abstract
Methamphetamine use disorder (MUD) is characterized by loss of control over compulsive drug use. Here, we used a self-administration (SA) model to investigate transcriptional changes associated with the development of early and late compulsivity during contingent footshocks. Punishment initially separated methamphetamine taking rats into always shock-resistant (ASR) rats that continued active lever pressing and shock-sensitive (SS) rats that reduced their lever pressing. At the end of the punishment phase, rats underwent 15 days of forced abstinence at the end of which they were re-introduced to the SA paradigm followed by SA plus contingent shocks. Interestingly, 36 percent of the initial SS rats developed delayed shock-resistance (DSR). Of translational relevance, ASR rats showed more incubation of methamphetamine craving than DSR and always sensitive (AS) rats. RNA sequencing revealed increased striatal Rab37 and Dipk2b mRNA levels that correlated with incubation of methamphetamine craving. Interestingly, Bdnf mRNA levels showed HDAC2-dependent decreased expression in the AS rats. The present SA paradigm should help to elucidate the molecular substrates of early and late addiction-like behaviors., (© 2024. This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply.)
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- 2024
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110. Footshock-Induced Abstinence from Compulsive Methamphetamine Self-administration in Rat Model Is Accompanied by Increased Hippocampal Expression of Cannabinoid Receptors (CB1 and CB2).
- Author
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Jayanthi S, Peesapati R, McCoy MT, Ladenheim B, and Cadet JL
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- Animals, Compulsive Behavior, Hippocampus, Rats, Receptors, Cannabinoid, Central Nervous System Stimulants adverse effects, Methamphetamine adverse effects
- Abstract
Methamphetamine (METH) use disorder (MUD) is characterized by compulsive and repeated drug taking despite negative life consequences. Large intake of METH in humans and animals is accompanied by dysfunctions in learning and memory processes. The endocannabinoid system (ECS) is known to modulate synaptic plasticity and cognitive functions. In addition, the ECS has been implicated in some of the manifestations of substance use disorders (SUDs). We therefore sought to identify potential changes in the expression of various enzymes and of the receptors (CB1 and CB2) that are members of that system. Herein, we used a model of METH self-administration (SA) that includes a punishment phase (footshocks) that helps to separate rats into a compulsive METH phenotype (compulsive) that continues to take METH and a non-compulsive METH (abstinent) group that suppressed or stopped taking METH. Animals were euthanized 2 h after the last METH SA session and their hippocampi were used to measure mRNA levels of cannabinoid receptors (CB/Cnr), as well as those of synthesizing (DAGL-A, DAGL-B, NAPEPLD) and metabolizing (MGLL, FAAH, PTGS2) enzymes of the endocannabinoid cascade. Non-compulsive rats exhibited significant increased hippocampal expression of CB1/Cnr1 and CB2/Cnr2 mRNAs. mRNA levels of the synthesizing enzyme, DAGL-A, and of the metabolic enzymes, MGLL and FAAH, were also increased. Non-compulsive rats also exhibited a significant decrease in hippocampal Ptgs2 mRNA levels. Taken together, these observations implicate the hippocampal endocannabinoid system in the suppression of METH intake in the presence of adverse consequences., (© 2021. This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply.)
- Published
- 2022
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111. Elevated body fat increases amphetamine accumulation in brain: evidence from genetic and diet-induced forms of adiposity.
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Fu X, Shah AP, Keighron J, Mou TM, Ladenheim B, Alt J, Fukudome D, Niwa M, Tamashiro KL, Tanda G, Sawa A, Cadet JL, Rais R, and Baraban JM
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- Adipose Tissue, Animals, Brain, Diet, High-Fat, Humans, Mice, Mice, Inbred C57BL, Mice, Knockout, Obesity, Adiposity, Amphetamine pharmacology
- Abstract
Despite the high prevalence of obesity, little is known about its potential impact on the pharmacokinetics of psychotropic drugs. In the course of investigating the role of the microRNA system on neuronal signaling, we found that mice lacking the translin/trax microRNA-degrading enzyme display an exaggerated locomotor response to amphetamine. As these mice display robust adiposity in the context of normal body weight, we checked whether this phenotype might reflect elevated brain levels of amphetamine. To assess this hypothesis, we compared plasma and brain amphetamine levels of wild type and Tsn KO mice. Furthermore, we checked the effect of diet-induced increases in adiposity on plasma and brain amphetamine levels in wild type mice. Brain amphetamine levels were higher in Tsn KO mice than in wild type littermates and correlated with adiposity. Analysis of the effect of diet-induced increases in adiposity in wild type mice on brain amphetamine levels also demonstrated that brain amphetamine levels correlate with adiposity. Increased adiposity displayed by Tsn KO mice or by wild type mice fed a high-fat diet correlates with elevated brain amphetamine levels. As amphetamine and its analogues are widely used to treat attention deficit disorder, which is associated with obesity, further studies are warranted to assess the impact of adiposity on amphetamine levels in these patients., (© 2021. The Author(s).)
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- 2021
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112. Oxycodone self-administration activates the mitogen-activated protein kinase/ mitogen- and stress-activated protein kinase (MAPK-MSK) signaling pathway in the rat dorsal striatum.
- Author
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Blackwood CA, McCoy MT, Ladenheim B, and Cadet JL
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- Animals, Blotting, Western, Male, RNA, Messenger metabolism, Rats, Rats, Sprague-Dawley, Receptors, AMPA metabolism, Receptors, Glutamate metabolism, Signal Transduction drug effects, Mitogen-Activated Protein Kinases metabolism, Oxycodone pharmacology
- Abstract
To identify signaling pathways activated by oxycodone self-administration (SA), Sprague-Dawley rats self-administered oxycodone for 20 days using short-(ShA, 3 h) and long-access (LgA, 9 h) paradigms. Animals were euthanized 2 h after SA cessation and dorsal striata were used in post-mortem molecular analyses. LgA rats escalated their oxycodone intake and separated into lower (LgA-L) or higher (LgA-H) oxycodone takers. LgA-H rats showed increased striatal protein phosphorylation of ERK1/2 and MSK1/2. Histone H3, phosphorylated at serine 10 and acetylated at lysine 14 (H3S10pK14Ac), a MSK1/2 target, showed increased abundance only in LgA-H rats. RT-qPCR analyses revealed increased AMPA receptor subunits, GluA2 and GluA3 mRNAs, in the LgA-H rats. GluA3, but not GluA2, mRNA expression correlated positively with changes in pMSK1/2 and H3S10pK14Ac. These findings suggest that escalated oxycodone SA results in MSK1/2-dependent histone phosphorylation and increases in striatal gene expression. These observations offer potential avenues for interventions against oxycodone addiction.
- Published
- 2021
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113. Neurochemical and behavioral comparisons of contingent and non-contingent methamphetamine exposure following binge or yoked long-access self-administration paradigms.
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Schweppe CA, Burzynski C, Jayanthi S, Ladenheim B, Cadet JL, Gardner EL, Xi ZX, van Praag H, Newman AH, and Keck TM
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- 3,4-Dihydroxyphenylacetic Acid metabolism, Animals, Cognition physiology, Dopamine metabolism, Dopamine Plasma Membrane Transport Proteins metabolism, Hydroxyindoleacetic Acid metabolism, Male, Maze Learning physiology, Memory Disorders chemically induced, Memory Disorders metabolism, Rats, Rats, Wistar, Self Administration psychology, Central Nervous System Stimulants administration & dosage, Cognition drug effects, Hippocampus drug effects, Hippocampus metabolism, Maze Learning drug effects, Methamphetamine administration & dosage
- Abstract
Rationale: Abuse of the psychostimulant methamphetamine (METH) can cause long-lasting damage to brain monoaminergic systems and is associated with profound mental health problems for users, including lasting cognitive impairments. Animal models of METH exposure have been useful in dissecting the molecular effects of the drug on cognition, but many studies use acute, non-contingent "binge" administrations of METH which do not adequately approximate human METH use. Long-term METH exposure via long-access (LgA) self-administration paradigms has been proposed to more closely reflect human use and induce cognitive impairments., Objective: To better understand the role of contingency and patterns of exposure in METH-induced cognitive impairments, we analyzed behavioral and neurochemical outcomes in adult male rats, comparing non-contingent "binge" METH administration with contingent (LgA) METH self-administration and non-contingent yoked partners., Results: Binge METH (40 mg/kg, i.p., over 1 day) dramatically altered striatal and hippocampal dopamine, DOPAC, 5-HT, 5-HIAA, BDNF, and TrkB 75 days after drug exposure. In contrast, 6-h LgA METH self-administration (cumulative 24.8-48.9 mg METH, i.v., over 16 days) altered hippocampal BDNF in both contingent and yoked animals but reduced striatal 5-HIAA in only contingent animals. Neurochemical alterations following binge METH administration were not accompanied by cognitive deficits in Morris water maze, novel object recognition, or Y-maze tests. However, contingent LgA METH self-administration resulted in impaired spatial memory in the water maze., Conclusions: Overall, substantial differences in neurochemical markers between METH exposure and self-administration paradigms did not consistently translate to deficits in cognitive tasks, highlighting the complexity of correlating METH-induced neurochemical changes with cognitive outcomes.
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- 2020
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114. A Single Prior Injection of Methamphetamine Enhances Methamphetamine Self-Administration (SA) and Blocks SA-Induced Changes in DNA Methylation and mRNA Expression of Potassium Channels in the Rat Nucleus Accumbens.
- Author
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Jayanthi S, Torres OV, Ladenheim B, and Cadet JL
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- Animals, Central Nervous System Stimulants pharmacology, Nucleus Accumbens metabolism, Potassium Channels drug effects, RNA, Messenger metabolism, Rats, Self Administration, DNA Methylation drug effects, Methamphetamine pharmacology, Nucleus Accumbens drug effects, Potassium Channels metabolism
- Abstract
The transition from occasional to escalated psychostimulant use is accelerated by prior drug exposure. These behavioral observations may be related to long-lasting transcriptional and/or epigenetic changes induced by the drug pre-exposure. Herein, we investigated if a single methamphetamine (METH) injection would enhance METH self-administration (SA) and impact any METH SA-induced epigenetic or transcriptional alterations. We thus injected a single METH dose (10 mg/kg) or saline to rats before training them to self-administer METH or saline. There were three experimental groups in SA experiments: (1) a single saline injection followed by saline SA (SS); (2) a single saline injection followed by METH SA (SM); and (3) a single METH injection followed by METH SA (MM). METH-pretreated rats escalated METH SA earlier and took more METH than saline-pretreated animals. Both groups showed similar incubation of cue-induced METH craving. Because compulsive METH takers and METH-abstinent rats show differences in potassium (K
+ ) channel mRNA levels in their nucleus accumbens (NAc), we wondered if K+ channel expression might also help to distinguish between SM and MM groups. We found increases in mRNA and protein expression of shaker-related voltage-gated K+ channels (Kv1: Kcna1, Kcna3, and Kcna6) and calcium-activated K+ channels (Kcnn1) in the SM compared to MM rats. SM rats also showed decreased DNA methylation at the CpG-rich sites near the promoter region of Kcna1, Kcna3 and Kcnn1 genes in comparison to MM rats. Together, these results provide additional evidence for potentially using K+ channels as therapeutic targets against METH use disorder.- Published
- 2020
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115. Molecular Adaptations in the Rat Dorsal Striatum and Hippocampus Following Abstinence-Induced Incubation of Drug Seeking After Escalated Oxycodone Self-Administration.
- Author
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Blackwood CA, Hoerle R, Leary M, Schroeder J, Job MO, McCoy MT, Ladenheim B, Jayanthi S, and Cadet JL
- Subjects
- Animals, Down-Regulation drug effects, Male, RNA, Messenger genetics, RNA, Messenger metabolism, Rats, Sprague-Dawley, Receptors, Opioid, kappa genetics, Receptors, Opioid, kappa metabolism, Receptors, Opioid, mu genetics, Receptors, Opioid, mu metabolism, Time Factors, Adaptation, Physiological, Behavior, Addictive genetics, Corpus Striatum pathology, Hippocampus pathology, Oxycodone administration & dosage, Self Administration
- Abstract
Repeated exposure to the opioid agonist, oxycodone, can lead to addiction. Here, we sought to identify potential neurobiological consequences of withdrawal from escalated and non-escalated oxycodone self-administration in rats. To reach these goals, we used short-access (ShA) (3 h) and long-access (LgA) (9 h) exposure to oxycodone self-administration followed by protracted forced abstinence. After 31 days of withdrawal, we quantified mRNA and protein levels of opioid receptors in the rat dorsal striatum and hippocampus. Rats in the LgA, but not the ShA, group exhibited escalation of oxycodone SA, with distinction of two behavioral phenotypes of relatively lower (LgA-L) and higher (LgA-H) oxycodone takers. Both LgA, but not ShA, phenotypes showed time-dependent increases in oxycodone seeking during the 31 days of forced abstinence. Rats from both LgA-L and LgA-H groups also exhibited decreased levels of striatal mu opioid receptor protein levels in comparison to saline and ShA rats. In contrast, mu opioid receptor mRNA expression was increased in the dorsal striatum of LgA-H rats. Moreover, hippocampal mu and kappa receptor protein levels were both increased in the LgA-H phenotype. Nevertheless, hippocampal mu receptor mRNA levels were decreased in the two LgA groups whereas kappa receptor mRNA expression was decreased in ShA and LgA oxycodone groups. Decreases in striatal mu opioid receptor protein expression in the LgA rats may serve as substrates for relapse to drug seeking because these changes occur in rats that showed incubation of oxycodone seeking.
- Published
- 2019
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116. Calcineurin/NFAT-induced up-regulation of the Fas ligand/Fas death pathway is involved in methamphetamine-induced neuronal apoptosis.
- Author
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Jayanthi S, Deng X, Ladenheim B, McCoy MT, Cluster A, Cai NS, and Cadet JL
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- Active Transport, Cell Nucleus drug effects, Animals, Calcineurin, Corpus Striatum metabolism, Fas Ligand Protein, Male, NFATC Transcription Factors, Nerve Tissue Proteins metabolism, Neurons cytology, Neurons metabolism, Rats, Rats, Sprague-Dawley, Transcription Factors genetics, Transcription, Genetic drug effects, Up-Regulation, fas Receptor metabolism, Apoptosis drug effects, DNA-Binding Proteins metabolism, Membrane Glycoproteins metabolism, Methamphetamine pharmacology, Neurons drug effects, Nuclear Proteins metabolism, Transcription Factors metabolism
- Abstract
Methamphetamine [METH ("speed")] is an abused psychostimulant that can cause psychotic, cognitive, and psychomotor impairment in humans. These signs and symptoms are thought to be related to dysfunctions in basal ganglionic structures of the brain. To identify possible molecular bases for these clinical manifestations, we first used cDNA microarray technology to measure METH-induced transcriptional responses in the striatum of rats treated with an apoptosis-inducing dose of the drug. METH injection resulted in increased expression of members of the Jun, Egr, and Nur77 subfamilies of transcription factors (TFs), changes that were confirmed by quantitative PCR. Because pathways linked to these factors are involved in the up-regulation of Fas ligand (FasL), FasL mRNA was quantified and found to be increased. Immunohistochemical studies also revealed METH-induced increased FasL protein expression in striatal GABAergic neurons that express enkephalin. Moreover, there were METH-mediated increases in calcineurin, as well as shuttling of nuclear factor of activated T cells (NFAT)c3 and NFATc4 from the cytosol to the nucleus of METH-treated rats, mechanisms also known to be involved in FasL regulation. Furthermore, METH induced cleavage of caspase-3 in FasL- and Fas-containing neurons. Finally, the METH-induced changes in the FasL-Fas death pathway were attenuated by pretreatment with the dopamine D1 receptor antagonist, SCH23390, which also caused attenuation of METH-induced apoptosis. These observations indicate that METH causes some of its neurodegenerative effects, in part, via stimulation of the Fas-mediated cell death pathway consequent to FasL up-regulation mediated by activation of multiple TFs.
- Published
- 2005
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117. Chronic cocaine increases kappa-opioid receptor density: lack of effect by selective dopamine uptake inhibitors.
- Author
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Collins SL, Kunko PM, Ladenheim B, Cadet JL, Carroll FI, and Izenwasser S
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- Animals, Caudate Nucleus drug effects, Caudate Nucleus metabolism, Drug Administration Schedule, Infusion Pumps, Implantable, Injections, Subcutaneous, Male, Nucleus Accumbens drug effects, Nucleus Accumbens metabolism, Piperazines administration & dosage, Putamen drug effects, Putamen metabolism, Rats, Rats, Sprague-Dawley, Receptors, Opioid, kappa biosynthesis, Cocaine administration & dosage, Cocaine analogs & derivatives, Dopamine Uptake Inhibitors administration & dosage, Receptors, Opioid, kappa metabolism
- Abstract
Continuous infusion of cocaine or the selective dopamine uptake inhibitors GBR 12909 or RTI-117 increases locomotor stimulation, to which partial tolerance occurs. In addition, all three drugs produce significant decreases in tyrosine hydroxylase immunoreactivity in caudate putamen and nucleus accumbens core, suggesting a decreased dopaminergic tone. An interaction between cocaine and opioids has long been documented. Chronic cocaine significantly increases mu and kappa-opioid receptors and treatment with a kappa-opioid agonist markedly reduces the behavioral effects of cocaine. In addition, chronic cocaine, but not GBR 12909, increases prodynorphin gene expression in caudate putamen. To further understand the interaction between cocaine and the kappa-opioid system, the effects of a chronic continuous infusion for 14 days of cocaine or one of the selective dopamine uptake inhibitors GBR 12909 or RTI-117 via osmotic minipump were examined on kappa-opioid receptors using the selective kappa-opioid ligand [3H] U-69593. [3H] U-69593 binding density was significantly increased in caudate putamen, nucleus accumbens shell, claustrum, and endopiriform nucleus after cocaine, while neither GBR 12909 nor RTI-117 had any effect. The increased kappa-opioid receptor densities observed following cocaine are likely not related to dopamine uptake inhibition, since they were not produced by selective dopamine uptake inhibitors. These findings suggest that regulation of kappa-opioid receptors by cocaine may be via inhibition of serotonin or norepinephrine uptake, by a combination of effects on two or three monoamine transporters, or by a mechanism unrelated to transporter inhibition., (Copyright 2002 Wiley-Liss, Inc.)
- Published
- 2002
- Full Text
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