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Double fertilization is a key determinant of grain yield, and the failure of fertilization during hybridization is one important reason for reproductive isolation. Therefore, fertilization has a very important role in the production of high-yield and well-quality hybrid of rice. Here, we used RNA sequencing technology to study the change of the transcriptome during double fertilization with the help of the mutant fertilization barrier (feb) that failed to finish fertilization process and led to seed abortion. The results showed that 1669 genes were related to the guided growth of pollen tubes, 332 genes were involved in the recognition and fusion of the male–female gametes, and 430 genes were associated with zygote formation and early free endosperm nuclear division. Among them, the genes related to carbohydrate metabolism; signal transduction pathways were enriched in the guided growth of pollen tubes, the genes involved in the photosynthesis; fatty acid synthesis pathways were activated by the recognition and fusion of the male–female gametes; and the cell cycle-related genes might play an essential role in zygote formation and early endosperm nuclear division. Furthermore, among the 1669 pollen tube-related genes, it was found that 7 arabinogalactan proteins (AGPs), 1 cysteine-rich peptide (CRP), and 15 receptor-like kinases (RLKs) were specifically expressed in anther, while 2 AGPs, 7 CRPs, and 5 RLKs in pistil, showing obvious unequal distribution which implied they might play different roles in anther and pistil during fertilization. These studies laid a solid foundation for revealing double fertilization mechanism of rice and for the follow-up investigation.

The volume of a black hole under noncommutative spacetime background is found to be infinite, in contradiction with the surface area of a black hole, or its Bekenstein–Hawking (BH) entropy, which is well-known to be finite. Our result rules out the possibility of interpreting the entropy of a black hole by counting the number of modes wrapped inside its surface if the final evaporation stage can be properly treated. It implies the statistical interpretation for the BH entropy can be independent of the volume, provided spacetime is noncommutative. The effect of radiation back reaction is found to be small and doesn't influence the above conclusion.

For violent vibration of the sink roll device of a hot-dip galvanizing line, an incomplete liquid-solid coupling method based on the wet modal was studied, the vibration modal of the device was numerically simulated, the natural frequencies and vibration modes were discussed, and the vibration test was performed. The partial mode of the sink roll changed the assembly relationship in bearings, and the clipping appeared in the time domain waveform of measured signals, indicating that crashing and friction existed in the sliding bearings. The frequency of the peak in the amplitude spectrum was 45.05 Hz, close to the fourth order frequency and mode, which showed that the fourth order natural vibration of the device was dominant. The double-frequency vibration was caused by nonlinear vibration of liquid-solid coupling. The main cause of sidebands near the peak in the amplitude spectrum came from the continuous action of the zinc liquid flow field. The numerical analysis and experimented research have significant correlation, and the incomplete solid-liquid coupling method can be effectively applied to engineering practice.

Sacbrood virus (SBV) is one of the most common and harmful viruses to honeybees. It causes failure to pupate and death during larval stage, in adult bees it has an influence on their behavior and even shortens their life-span. In this study, we analyzed the phylogenetic relationships among the SBV isolates from all around the world, with from both Apis cerana and Apis mellifera. Phylogenetic trees were constructed based on three types of nucleotide sequences: complete genome sequence, VP1 gene and SB1-2 fragment of SBV. Moreover, genome recombination analysis was performed to assess the effect of genome recombination on the evolutionary relationship of some SBV isolates. The phylogenetic trees showed that although all the SBV isolates form two major groups, these two groups were not formed strictly according to their host specificity or geographical origin. These results indicate that both host specificity and geographic origin decide the genetic diversity of SBV strains.

Background/Aims: Osteoporosis is a progressive bone disease characterized by a decrease in bone mass and density, which results in an increased risk of fractures. Mesenchymal stem cells (MSCs) are progenitor cells that can differentiate into osteoblasts, osteocytes and adipocytes in bone and fat formation. A reduction in the differentiation of MSCs into osteoblasts contributes to the impaired bone formation observed in osteoporosis. MicroRNAs (miRNAs) play a regulatory role in osteogenesis and MSC differentiation. MiR-27a has been reported to be down-regulated in the development of osteoporosis and during adipogenic differentiation. Methods: In this study, a miRNA microarray analysis was used to investigate expression profiles of miRNA in the serum of osteoporotic patients and healthy controls and this data was validated by quantitative real-time PCR (qRT-PCR). MSCs isolated from human and mice with miR-27a inhibition or overexpression were induced to differentiate into osteoblasts or adipocytes. TargetScan and PicTar were used to predict the target gene of miR-27a. The mRNA or protein levels of several specific proteins in MSCs were detected using qRT-PCR or western blot analysis. Ovariectomized mice were used as in vivo model of human postmenopausal osteoporosis for bone mineral density measurement, micro-CT analysis and histomorphometric analysis. Results: Here, we analyzed the role of miR-27a in bone metabolism. Microarray analysis indicated that miR-27a expression was significantly reduced in osteoporotic patients. Analysis on MSCs derived from patients with osteoporosis indicated that osteoblastogenesis was reduced, whereas adipogenesis was increased. MSCs that had undergone osteoblast induction showed a significant increase in miR-27a expression, whereas cells that had undergone adipocyte induction showed a significant decrease in miR-27a expression, indicating that miR-27a was essential for MSC differentiation. We demonstrated that myocyte enhancer factor 2 c (Mef2c), a transcription factor, was the direct target of miR-27a using a dual luciferase assay. An inverse relationship between miR-27a expression and Mef2c expression in osteoporotic patients was shown. Silencing of miR-27a decreased bone formation, confirming the role of miR-27a in bone formation in vivo. Conclusion: In summary, miR-27a was essential for the shift of MSCs from osteogenic differentiation to adipogenic differentiation in osteoporosis by targeting Mef2c.

We present a necessary and sufficient condition to falsify whether a Hawking radiation spectrum indicates unitary emission process or not from the perspective of information theory. With this condition, we show the precise values of Bekenstein–Hawking entropies for Schwarzschild black holes and Reissner–Nordström black holes can be calculated by counting the microstates of their Hawking radiations. In particular, for the extremal Reissner–Nordström black hole, its number of microstate and the corresponding entropy we obtain are found to be consistent with the string theory results. Our finding helps to refute the dispute about the Bekenstein–Hawking entropy of extremal black holes in the semiclassical limit.

Herein, we focus on energy efficiency optimization for massive multiple-input multiple-output (MIMO) downlink secure multicast transmission exploiting statistical channel state information (CSI). Privacy engineering in the field of communication is a hot issue under study. The common signal transmitted by the base station is multicast transmitted to multiple legitimate user terminals in our system, but an eavesdropper might eavesdrop this signal. To achieve the energy efficiency utility–privacy trade-off of multicast transmission, we set up the problem of maximizing the energy efficiency which is defined as the ratio of the secure transmit rate to the power consumption. To simplify the formulated nonconvex problem, we use a lower bound of the secure multicast rate as the molecule of the design objective. We then obtain the eigenvector of the optimal transmit covariance matrix into a closed-form, simplifying the matrix-valued multicast transmission strategy problem into a power allocation problem in the beam domain. By utilizing the Minorize-Maximize method, an iterative algorithm is proposed to decompose the secure energy efficiency optimization problem into a sequence of iterative fractional programming subproblems. By using Dinkelbach’s transform, each subproblem becomes an iterative problem with the concave objective function, and it can be solved by classical convex optimization. We guarantee the convergence of the two-level iterative algorithm that we propose. Besides, we reduce the computational complexity of the algorithm by substituting the design objective with its deterministic equivalent. The numerical results show that the approach we propose performs well compared with the conventional methods.

In this work, we propose a satellite-aided permissionless consensus protocol for scalable space–terrestrial blockchains. We design its working principle and workflow by taking full advantage of satellites for extensive coverage and ubiquitous connectivity. Based on the proposed protocol, we demonstrate how such a space–terrestrial blockchain grows and evolves through several typical cases in the presence of adversarial nodes, user misbehavior, and transmission outage. Taking proof of work (PoW) as a benchmark, we assess the system security by considering both adversarial miners and possible colluding satellites. Then, we analyze the maximum blockchain throughput under network capacity limits and evaluate the impact of information propagation delay via a Markov model. Simulation results support that the proposed satellite-aided consensus protocol achieves higher throughput and exhibits greater scalability than PoW.

As the core technology of 5G mobile communication systems, massive multi-input multi-output (MIMO) can dramatically enhance the energy efficiency (EE), as well as the spectral efficiency (SE), which meets the requirements of new applications. Meanwhile, physical layer multicast technology has gradually become the focus of next-generation communication technology research due to its capacity to efficiently provide wireless transmission from point to multipoint. The availability of channel state information (CSI), to a large extent, determines the performance of massive MIMO. However, because obtaining the perfect instantaneous CSI in massive MIMO is quite challenging, it is reasonable and practical to design a massive MIMO multicast transmission strategy using statistical CSI. In this paper, in order to optimize the system resource efficiency (RE) to achieve EE-SE balance, the EE-SE trade-offs in the massive MIMO multicast transmission are investigated with statistical CSI. Firstly, we formulate the eigenvectors of the RE optimization multicast covariance matrices of different user terminals in closed form, which illustrates that in the massive MIMO downlink, optimal RE multicast precoding is supposed to be done in the beam domain. On the basis of this viewpoint, the optimal RE precoding design is simplified into a resource efficient power allocation problem. Via invoking the quadratic transform, we propose an iterative power allocation algorithm, which obtains an adjustable and reasonable EE-SE tradeoff. Numerical simulation results reveal the near-optimal performance and the effectiveness of our proposed statistical CSI-assisted RE maximization in massive MIMO.

Estimating multiple parameters simultaneously is of great importance to measurement science and application. For a single parameter, atomic Ramsey interferometry (or equivalently optical Mach–Zehnder interferometry) is capable of providing the precision at the standard quantum limit (SQL) using unentangled probe states as input. In such an interferometer, the first beam splitter represented by unitary transformation U generates a quantum phase sensing superposition state, while the second beam splitter U ^−1 recombines the phase encoded paths to realize interferometric sensing in terms of population measurements. We prove that such an interferometric scheme can be directly generalized to estimation of multiple parameters (associated with commuting generators) to the SQL precision using multi-mode unentangled states, if (but not iff) U is orthogonal, i.e. a unitary transformation with only real matrix elements. We show that such a U can always be constructed experimentally in a simple and scalable manner. The effects of particle number fluctuation and detection noise on such multi-mode interferometry are considered. Our findings offer a simple solution for estimating multiple parameters corresponding to mutually commuting generators.

Heat treatment was used to extend the shelf life of postharvest Chaenomeles speciosa (Sweet) Nakai (C. speciosa), its effects on the quality and physiological properties of C. speciosa were studied. The results showed that heat treatment reduced the weight loss, inhibited the decline of titratable acids (TA), maintained a higher activity of peroxidase (POD) and catalase (CAT), compared to untreated C. speciosa. Treatment in hot water dipping at 50℃ for 2-4 min maintained the better quality of postharvest C. speciosa stored at 0℃ for 120 days, which indicated that heat treatment is an effective preservation technology to prolong the shelf life of C. speciosa.

Paracaesicola n. gen., is erected herein to accommodate a new microcotylid species, Paracaesicola nanshaensis n. sp., collected from the Yongshu Reef, South China Sea. This species is the first monogenean to be recorded from the gills of Paracaesio sordida. The new species is characterized by the following features: (i) haptor short, with clamps arranged in two equal bilateral rows; (ii) testes numerous, arranged in two roughly alternating longitudinal rows, extending into the haptor; (iii) genital atrium armed with 16 robust spines, which are vertically arranged on top of the sausage shaped muscular male copulatory organ; and (iv) single vagina, bottle-shaped, with a distinctly bulbous vaginal atrium. The terminals of the reproductive system discriminate Paracaesicola n. gen. from all other genera in the Microcotylidae. Molecular phylogenetic analyses, based on partial 28S rDNA, places Paracaesicola nanshaensis n. sp. within the microcotylid clade, but its sequence differs from all known available microcotylid sequences.

Parasite biodiversity of fish in coral reefs of the South China Sea is still incompletely explored. We describe here a new species of Neohexostoma (Monogenea: Hexostomatidae) from the gill filaments of the dogtooth tuna Gymnosarda unicolor (Scombridae), collected off Yongshu Reef, South China Sea. Neohexostoma gymnosardae n. sp. is distinguished from its congeners by the following features: (i) haptor clearly marked from body proper by a strongly constricted peduncle, divided in its posterior margin into two symmetrical lobes, (ii) vagina armed with scattered small blunt spines, (iii) eggs tied by their long polar filaments, (vi) esophagus with several lateral diverticula, (v) intestinal ceca unfused and extending into the haptor. We present an analysis of the relationships of this monogenean based on partial 28S rDNA sequences. An identification key for species of Neohexostoma is provided. This is the first member of the genus Neohexostoma known to parasitize a species of Gymnosarda.

Replication factor C (RFC) is a multisubunit complex that opens the sliding clamp and loads it onto the DNA chain in an ATP-dependent manner and is thus critical for high-speed DNA synthesis. In yeast (Saccharomyces cerevisiae) and humans, biochemical studies and structural analysis revealed interaction patterns between the subunits and architectures of the clamp loaders. Mutations of ScRFC1/2/3/4/5 lead to loss of cell viability and defective replication. However, the functions of RFC subunits in higher plants are unclear, except for AtRFC1/3/4, and the interaction and arrangement of the subunits have not been studied. Here, we identified rfc2-1/+, rfc3-2/+, and rfc5-1/+ mutants in Arabidopsis, and found that embryos and endosperm arrested at the 2/4-celled embryo proper stage and 6-8 nuclei stages, respectively. Subcellular localization analysis revealed that AtRFC1 and OsRFC1/4/5 proteins were localized in the nucleus, while AtRFC2/3/4/5 and OsRFC2/3 proteins were present both in the nucleus and cytoplasm. By using yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) techniques, we demonstrated the interactions of Arabidopsis and rice (Oryza sativa) RFC subunits, and proposed arrangements of the five subunits within the RFC complex, which were AtRFC5-AtRFC4-AtRFC3/2-AtRFC2/3-AtRFC1 and OsRFC5-OsRFC2-OsRFC3-OsRFC4-OsRFC1, respectively. In addition, AtRFC1 could interact with AtRFC2/3/4/5 in the presence of other subunits, while OsRFC1 directly interacted with the other four subunits. To further characterize the regions required for complex formation, truncated RFC proteins of the subunits were created. The results showed that C-termini of the RFC subunits are required for complex formation. Our studies indicate that the localization and interactions of RFCs in Arabidopsis and rice are distinctly discrepant.

Aiming at the existing characters and deficiencies of the drilling experiment platform, a new kind of multifunctional drilling comprehensive experiment platform was designed. First of all,a new way to balance the drill rig load based on its design was put forward. Then by the internal and external balance methods,the results were calculated and compared,and the different effects on the experiment platform performance were obtained. Finally,according to the design characteristics of the drilling comprehensive experimental platform that could drill obliquely,the load mechanical property of the pit shaft was analyzed at different balance conditions,and the key technical parameters of the experiment platform design was got.The results show that the portal frame or pit shaft can support the drill pipe axial load in different balance ways. In essence,the load is transferred or transformed between the portal frame,pit shaft,track and foundation. Considering from the point of safety,using the scheme of the portal frame under load（ external balance mechanical system） is superior to the internal balance scheme of the pit shaft. The research method and conclusions have reference significance for optimal design,failure analysis and safety evaluation.