101. Dammarane-Type Triterpenoids from the Roots of Rhus chinensisand Their Preventive Effects on Zebrafish Heart Failure and Thrombosis
- Author
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Ye, Miao, Xu, Wen, He, Da-Qing, Wu, Xian, Lai, Wen-Fang, Zhang, Xiao-Qin, Lin, Yu, Xu, Wei, and Li, Xu-Wen
- Abstract
Eight new dammarane-type triterpenoids (1–8), together with a related known analogue (9), were isolated from the roots of Rhus chinensis, a traditional Chinese medicine for treating coronary artery heart disease, guided by LC-MS analysis. Their structures were elucidated based on extensive spectroscopic analysis and quantum chemical calculations. Notably, compounds 1–7and 9possess an unusual 17α-side chain, and 1–4, 6, and 9contain an uncommon 3-methyl-5,6-dihydro-2H-pyran-2-one moiety in the side chain. Compounds 1–5and 9have a 3,19-hemiketal bridge in the A ring. In an in vivo bioassay, 1, 2, and 4–6exhibited significant preventive effects on zebrafish heart failure at 0.5 μg/mL, improving heart dilatation, venous congestion, cardiac output, blood flow velocity, and heart rate. Compound 5, displaying the most promising heart failure preventive activities, showed even better effects on increasing cardiac output (72%) and blood flow velocity (83%) than six first-line heart failure therapeutic drugs. Moreover, 1, 2, and 6prevented the formation of thrombosis in zebrafish at 0.5 μg/mL. The present investigation suggests that the new dammarane triterpenoids might be partially responsible for the utility of R. chinensisin treating coronary artery heart disease.
- Published
- 2020
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