127 results on '"Li, Zeyun"'
Search Results
102. Assessing the Driving Forces Influencing World City Formation in Shanghai Based upon PLS-SEM Approach
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Li, Zeyun, primary and Dawood, Sharifah Rohayah Sheikh, additional
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- 2016
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103. World Cities Formation in China: A Comparative Study of Five Pre-Eminent Cities
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Li, Zeyun, primary and Sheikh Dawood, Sharifah Rohayah, additional
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- 2016
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104. Determination of five nucleosides by LC‐MS/MS and the application of the method to quantify N6‐methyladenosine level in liver messenger ribonucleic acid of an acetaminophen‐induced hepatotoxicity mouse model.
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Liu, Ruijuan, Zhao, Fang, Wei, Jingyao, Yu, Pei, Zhang, Ji, Wang, Yueqin, Li, Zeyun, Zhang, Jiali, Zhang, Xiaojian, and Tian, Xin
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MESSENGER RNA ,NUCLEOSIDES ,RNA ,HIGH performance liquid chromatography ,TANDEM mass spectrometry ,URIDINE ,LIVER analysis - Abstract
Ribonucleic acid N6‐methyladenosine methylation plays an important role in a variety of biological processes and diseases. Acetaminophen‐induced hepatotoxicity is one of the major challenges faced by clinicians. To date, the link between N6‐methyladenosine and acetaminophen‐induced hepatotoxicity has not been studied. In this study, a simple ultra high performance liquid chromatography with tandem mass spectrometry method was developed for the simultaneous determination of five nucleosides (adenosine, uridine, cytidine, guanosine, and N6‐methyladenosine) in messenger ribonucleic acid. After enzymatic digestion of messenger ribonucleic acid, the nucleosides sample was separated on an Acquity UPLC column with gradient elution using methanol and 0.02% formic acid water, and detected by a Qtrap 4500 mass spectrometer with an electrospray ionization mode. The method was validated over the concentration ranges of 4–800 ng/mL for adenosine, uridine, cytidine, and guanosine and 0.1–20 ng/mL for N6‐methyladenosine. It was successfully applied to the determination of N6‐methyladenosine levels in liver messenger ribonucleic acid in an acetaminophen‐induced hepatotoxicity mouse model and a control group. This study offers a method for the determination of nucleoside contents in epigenetic studies and constitutes the first step toward the investigation of ribonucleic acid methylation in acetaminophen‐induced hepatotoxicity, which will facilitate the elucidation of its mechanism. [ABSTRACT FROM AUTHOR]
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- 2019
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105. Identification of metabolites of evobrutinib in rat and human hepatocytes by using ultra‐high performance liquid chromatography coupled with diode array detector and Q Exactive Orbitrap tandem mass spectrometry.
- Author
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Li, Zeyun, Zhang, Lizhen, Yuan, Yongliang, and Yang, Zhiheng
- Abstract
Evobrutinib is a highly selective inhibitor of Bruton's tyrosine kinase (BTK) which may be clinically effective in treating certain autoimmune diseases. The purpose of the present study was to investigate the metabolism of evobrutinib in rat and human hepatocytes. Evobrutinib was incubated with rat and human hepatocytes at 37°C for 2 hours after which the samples were analyzed by ultra‐high performance liquid chromatography with diode array detection and Q Exactive Orbitrap tandem mass spectrometry (UPLC–DAD–Q Exactive Orbitrap‐MS). The acquired data were processed by MetWorks™ software using mass effect filter and background subtraction functions. Under these conditions, 23 metabolites were detected and their identities proposed. Among these metabolites, M13 and M15 were identified by comparison of their retention times, accurate masses, and fragment ions with those of authentic reference standards. The metabolic pathways of evobrutinib were proposed accordingly. Our results demonstrated that evobrutinib was metabolized via hydroxylation, hydrolysis, O‐dealkylation, glucuronidation, and GSH conjugation. Species‐related metabolic differences between rat and human hepatocytes were observed. M1–M4 were rat‐specific metabolites. M13 (hydroxyl‐evobrutinib) was the major metabolite whereas M15 (evobrutinib‐diol) was a minor metabolite in rat hepatocytes. On the other hand, M6, M11, M16, M17, and M19 were human‐specific metabolites. M15 was the most abundant metabolite whereas M13 was the minor metabolite in human hepatocytes. This study provides preliminary information regarding the metabolism of evobrutinib that may be helpful in understanding the pharmacology of evobrutinib. To investigate the metabolic profiles of evobrutinib in human and rat hepatocytes, a strategy based on UPLC‐DAD‐Q Exactive Orbitrap‐MS was developed. Using this approach, 23 metabolites were structurally identified. This study provided overview of evobrutinib metabolic pathways and enabled the investigation of species‐related metabolic difference. [ABSTRACT FROM AUTHOR]
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- 2019
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106. World City Network in China: A Network Analysis of Air Transportation Network
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Li, Zeyun, primary and Dawood, Sharifah Rohayah Sheikh, additional
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- 2016
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107. Detection of free hemoglobin in blood products using transmission spectra and fluorescence spectra for quality assurance
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Lin, Ling, primary, Li, Zeyun, additional, Zhang, Linna, additional, Ren, Jing, additional, Fu, Zhigang, additional, Guan, Yang, additional, and Li, Gang, additional
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- 2016
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108. Multi-pathlength method to improve the spectrometric analysis accuracy based on “M + N” theory
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Li, Gang, primary, Luo, YongShun, additional, Li, Zhe, additional, Li, ZeYun, additional, and Lin, Ling, additional
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- 2016
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109. 1H-NMR Based Serum Metabolomics Study to Investigate Hepatoprotective Effect of Qin-Jiao on Carbon Tetrachloride-Induced Acute Hepatotoxicity in Rats.
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Li, Zeyun, Li, Ying, Lu, Lingpan, Yang, Zhiheng, Xue, Wenhua, Tian, Xin, and Zhang, Xiaojian
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- *
JAUNDICE treatment , *ALTERNATIVE medicine , *ANIMAL experimentation , *HERBAL medicine , *LIVER diseases , *CHINESE medicine , *METABOLISM , *MICE , *MULTIVARIATE analysis , *HEALTH outcome assessment , *PHYTOCHEMICALS , *IN vitro studies - Abstract
Gentiana macrophylla Radix, commonly known as Qin-Jiao (QJ), was recorded alone to treat jaundice in Compendium of Materia Medica and has been frequently prescribed for treatment of liver disease in China. However, the underlying mechanism remains unknown. In the present work, QJ of 1,2 g/kg or silybin of 40 mg/kg (positive control) was orally given to rats for 7 days to verify the protective effect on acute liver damage induced by tetrachloride (CCl4). Together with serum biochemistry and histopathological examination, 1H-NMR based metabolomics work was carried out to investigate the efficacy. It turned out that QJ of 2 g/kg exerted comparable protective effect with positive control and partially recovered disturbed metabolism by CCl4. Multivariate analysis was conducted and metabolites altered significantly among groups were assigned and discussed, including betaine, glucose, lactate, creatine, and LDL/VLDL. Metabolic regulations involved in QJ or silybin treatment were as follows: tricarboxylic acid (TCA) cycle, synthesis of LDL/VLDL, and gluconeogenesis were enhanced, while betaine metabolism, glycolysis, creatine metabolism, synthesis of ketone bodies, amino acids metabolism, and β-oxidation of fatty acids were suppressed. For the first time hepatoprotective effect of QJ on acute liver damage was revealed by 1H-NMR based metabolomics, prompting understanding of the underlying mechanism. [ABSTRACT FROM AUTHOR]
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- 2017
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110. A quantitative 1H nuclear magnetic resonance (qHNMR) method for assessing the purity of iridoids and secoiridoids
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Li, Zeyun, primary, Welbeck, Edward, additional, Yang, Li, additional, He, Chunyong, additional, Hu, Haijun, additional, Song, Ming, additional, Bi, Kaishun, additional, and Wang, Zhengtao, additional
- Published
- 2015
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111. Brand extension evaluation: a perspective on customer equity
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Shao, Jingbo, primary, Li, Zeyun, additional, and He, Qingsong, additional
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- 2014
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112. Metabolic profiles of 20(S )-protopanaxadiol in rats after oral administration using ultra-performance liquid chromatography/quadrupole time-of-flight tandem mass spectrometry
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He, Chunyong, primary, Li, Jia, additional, Wang, Rufeng, additional, Li, Zeyun, additional, Annie Bligh, S. W., additional, Yang, Li, additional, and Wang, Zhengtao, additional
- Published
- 2014
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113. A Combined Approach of High-Throughput Sequencing and Degradome Analysis Reveals Tissue Specific Expression of MicroRNAs and Their Targets in Cucumber
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Mao, Weihua, primary, Li, Zeyun, additional, Xia, Xiaojian, additional, Li, Yadan, additional, and Yu, Jingquan, additional
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- 2012
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114. Metabolic profiles of 20( S)-protopanaxadiol in rats after oral administration using ultra-performance liquid chromatography/quadrupole time-of-flight tandem mass spectrometry.
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He, Chunyong, Li, Jia, Wang, Rufeng, Li, Zeyun, Annie Bligh, S. W., Yang, Li, and Wang, Zhengtao
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METABOLITES ,CHEMICAL ecology ,MASS (Physics) ,SPECTROMETRY ,BODY fluids - Abstract
RATIONALE 20( S)-Protopanaxadiol (PPD), a dammarane-type triterpenoid sapogenin, acts as the pharmacophore of ginsenosides which are considered as the principal bioactive components in Chinese ginseng. To fully understand the mechanism of action of PPD, it is important to study its metabolic profiles in vivo. METHODS Plasma, urine, fece and bile were collected after administration of PPD formulated in 0.5% aqueous Tween-80 to rats (150 mg/kg). Samples were analyzed by using a sensitive and reliable method based on ultra-performance liquid chromatography/quadrupole time-of-flight tandem mass spectrometry (UPLC/Q-TOF-MS/MS) in both positive and negative ion mode. The chemical structures of metabolites were elucidated by comparing the retention time, accurate molecular mass, and fragmentation patterns of analytes with those of PPD. RESULTS In total 29 metabolites, including 10 new metabolites (M20-M29), were tentatively identified and characterized. Among them, two metabolites (M3 and M4) were unambiguously identified by matching their retention times and fragmentation patterns with their standards. Principal metabolites, namely, 20, 24-oxide metabolites (M3 and M4), 26/27-carboxylic acid derivatives (M22 and M23) and a glucuronidated product (M28), were found in the rat plasma. CONCLUSIONS The results showed that phase I metabolites are monooxygenation, dioxygenation and oxidative dehydrogenation metabolites, and phase II metabolic pathways were demonstrated to be cysteine conjugation and glucuronidation. The newly identified metabolites are useful to understand the mechanism of elimination of PPD and, in turn, its effectiveness and toxicity. Copyright © 2014 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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115. Identification of SWI2/SNF2-Related 1 Chromatin Remodeling Complex (SWR1-C) Subunits in Pineapple and the Role of Pineapple SWR1 COMPLEX 6 (AcSWC6) in Biotic and Abiotic Stress Response.
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Jakada, Bello Hassan, Aslam, Mohammad, Fakher, Beenish, Greaves, Joseph G., Li, Zeyun, Li, Weimin, Lai, Linyi, Ayoade, Oyekunle Adenike, Cheng, Yan, Cao, Shijiang, Li, Gang, Hu, Jer-Ming, and Qin, Yuan
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PINEAPPLE ,ABIOTIC stress ,CHROMATIN ,TRANSGENIC plants ,MYCOSES ,IDENTIFICATION - Abstract
Chromatin remodeling complex orchestrates numerous aspects of growth and development in eukaryotes. SWI2/SNF2-Related 1 chromatin remodeling complex (SWR1-C) is a member of the SWI/SNF ATPase-containing chromatin remodeling complex responsible for the exchange of H2A for H2A.Z. In plants, SWR1-C plays a crucial role by transcriptionally regulating numerous biological and developmental processes. However, SWR1-C activity remains obscure in pineapple. Here, we aim to identify the SWR1-C subunits in pineapple. By genome-wide identification, we found a total of 11 SWR1-C subunits in the pineapple. The identified SWR1-C subunits were named and classified based on the sequence similarity and phylogenetic analysis. RNA-Seq analysis showed that pineapple SWR1-C subunits are expressed differentially in different organs and at different stages. Additionally, the qRT-PCR of pineapple SWR1-C subunits during abiotic stress exposure showed significant changes in their expression. We further investigated the functions of pineapple SWR1 COMPLEX 6 (AcSWC6) by ectopically expressing it in Arabidopsis. Interestingly, transgenic plants ectopically expressing AcSWC6 showed susceptibility to fungal infection and enhanced resistance to salt and osmotic stress, revealing its involvement in biotic and abiotic stress. Moreover, the complementation of mutant Arabidopsisswc6 by pineapple SWC6 suggested the conserved function of SWC6 in plants. [ABSTRACT FROM AUTHOR]
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- 2019
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116. Integrated serum metabolomics, 16S rRNA sequencing and bile acid profiling to reveal the potential mechanism of gentiopicroside against nonalcoholic steatohepatitis in lean mice.
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Li Z, Zhu X, Li C, Tang R, Zou Y, and Liu S
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- Animals, Mice, Male, Mice, Inbred C57BL, Liver drug effects, Liver metabolism, Disease Models, Animal, Iridoid Glucosides pharmacology, Non-alcoholic Fatty Liver Disease drug therapy, Non-alcoholic Fatty Liver Disease blood, Bile Acids and Salts metabolism, Metabolomics, RNA, Ribosomal, 16S genetics
- Abstract
Ethnopharmacological Relevance: Lean nonalcoholic steatohepatitis (NASH) poses a serious threat to public health worldwide. Herbs of the genus Gentiana have been used for centuries to treat hepatic disease or have been consumed for hepatic protection efficiency. Gentiopicroside (GPS), the main bioactive component of Gentiana herbs, has been shown to be beneficial for protecting the liver, improving intestinal disorders, modulating bile acid profiles, ameliorating alcoholic hepatosteatosis, and so on. It is plausible to speculate that GPS may hold potential as a therapeutic strategy for lean NASH. However, no related studies have been conducted thus far., Aim of the Study: The present work aimed to investigate the benefit of GPS on NASH in a lean mouse model., Materials and Methods: NASH in a lean mouse model was successfully established via a published method. GPS of 50 and 100 mg/kg were orally administered to verify the effect. Untargeted metabolomics, 16S rDNA sequencing and bile acid (BA) profiling, as well as qPCR and Western blotting analysis were employed to investigate the mechanism underlying the alleviating effect., Results: GPS significantly reduced the increase in serum biochemicals and liver index, and attenuated the accumulation of fat in the livers of lean mice with NASH. Forty-two potential biomarkers were identified by metabolomics analysis, leading to abnormal metabolic pathways of primary bile acid biosynthesis and fatty acid biosynthesis, which were subsequently rebalanced by GPS. A decreased Firmicutes/Bacteroidetes (F/B) ratio and disturbed BA related GM profiles were revealed in lean mice with NASH but were partially recovered by GPS. Furthermore, serum profiling of 23 BAs confirmed that serum BA levels were elevated in the lean model but downregulated by GPS treatment. Pearson correlation analysis validated associations between BA profiles, serum biochemical indices and related GM. qPCR and Western blotting analysis further elucidated the regulation of genes associated with liver lipid synthesis and bile acid metabolism., Conclusions: GPS may ameliorate steatosis in lean mice with NASH, regulating the metabolomic profile, BA metabolism, fatty acid biosynthesis, and BA-related GM. All these factors may contribute to its beneficial effect., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)
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- 2024
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117. IMPACT OF CLIMATE CHANGE ON COASTAL ECOSYSTEM AND OUTDOOR ACTIVITIES: A COMPARATIVE ANALYSIS AMONG FOUR LARGEST COASTLINE COVERING COUNTRIES.
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Zhang T, Liu H, Lu Y, Wang Q, Loh YC, and Li Z
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- Indonesia, Canada, Norway, Conservation of Natural Resources, Humans, Climate Change, Ecosystem
- Abstract
Climate change and coastal ecosystems have become challenging subjects for world sustainability. Humans, animals, and other ocean habitats are primarily affected by the harmful changes in climate. Coastal ecosystems support biodiversity and a wide range of species that serve as habitats for many commercially important fish species and enhance human activities in coastal areas. By engaging in coastal outdoor activities, individuals can experience numerous physical and mental health benefits, foster environmental awareness. This study provided valuable insights into the importance of coastal outdoor activities and their potential to improve our quality of life. This study undertook a challenging subject where we graphically and econometrically analyze the relationship and linkages among coastal indicators with other climate-concerning factors. The study comprises the ordinary regression and comparative analysis among the four largest coastline countries in the world. The study took a sample from Canada, Indonesia, Norway, and the Russian Federation from 1990 to 2022. The data is selected on a convenient basis. Results declared that each country has its unique challenges and opportunities in mitigating adverse climate change and retaining a sustainable coastal ecosystem. The study surprisingly revealed that climate change insignificantly affects the coastal ecosystem in Indonesia and the Russian Federation while it inversely affects the coastal ecosystem in Canada and Norway, showed that climate change on average declines coastal production by 0.0041922 and 0.0261104 in Canada and Norway respectively. The detailed review is given in the results section; however, the pooling analysis proved that at the aggregate level, a one percent increase in climate change caused a 0.02266-tonne decline in coastal ecosystems in the four largest coastline nations. There is a need for policies tend to increase CAP activities by implementing practical marine protected areas. Furthermore, scientific research and monitoring will be beneficial in restoring coastal sustainability., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)
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- 2024
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118. Investigating the efficacy and mechanisms of Jinfu'an decoction in treating non-small cell lung cancer using network pharmacology and in vitro and in vivo experiments.
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Peng H, Huang Z, Li P, Sun Z, Hou X, Li Z, Sang R, Guo Z, Wu S, and Cao Y
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- Animals, Mice, Humans, Lumican, Delta Catenin, Mice, Nude, Network Pharmacology, Phosphatidylinositol 3-Kinases, Proto-Oncogene Proteins c-akt, Quality of Life, Molecular Docking Simulation, Carcinoma, Non-Small-Cell Lung drug therapy, Lung Neoplasms drug therapy, Drugs, Chinese Herbal pharmacology, Drugs, Chinese Herbal therapeutic use
- Abstract
Ethnopharmacological Relevance: Jinfu'an Decoction (JFAD) is a traditional Chinese decoction used in lung cancer treatment to improve patient quality of life and survival. Previous research has established that JFAD has a significant therapeutic effect on non-small cell lung cancer (NSCLC), although the underlying molecular mechanisms have not been largely underexplored., Aim of the Study: We used network pharmacology to identify the putative active ingredients of JFAD and conducted experimental studies to determine the potential molecular mechanism of JFAD in NSCLC treatment., Materials and Methods: The herbal components in JFAD-containing serum were identified by ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS), and targets associated with the anti-lung cancer metastasis effects of JFAD were retrieved from various databases. The Database for Annotation, Visualization and Integrated Discovery (DAVID) was used to perform Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Next, the protein-protein interactions network and the "JFAD-Chemical Component-Target-KEGG Pathway" network were constructed. The network pharmacology findings were confirmed by in vitro and in vivo experiments. In vitro experiments were conducted to assess cell viability by CCK8 assay, cell cycle analysis by propidium iodide (PI) assay, and migration and invasion ability of cells by the transwell assay. In vivo experiments were performed to assess the efficacy of JFAD on the tumor by observing the growth of transplanted tumor models in nude mice and evaluated by in vivo bioluminescence imaging. Moreover, we assessed the effect of JFAD on the PI3K/Akt signaling pathway and proteins of Lumican, p120ctn, and specific RhoGTP enzyme family members (RhoA, Rac1, and RhoC) by Western Blot and immunohistochemistry., Results: 32 herbal components were identified in the JFAD-containing serum, which potentially acted on 229 targets related to lung cancer metastasis. Network pharmacology results suggested that JFAD may treat lung cancer metastasis by targeting the PI3K/Akt pathway via regulating multiple core targets. Our experiments showed that JFAD suppressed the proliferation of A549 cells in vitro, induced cell cycle arrest, and reduced the migration and invasion ability of A549 cells. Our in vivo study revealed that JFAD inhibited tumor growth in a nude mouse model. Additionally, we found that JFAD could downregulate the expression of the PI3K/Akt pathway and affect the expression of Lumican, p120ctn, and specific RhoGTPase family members., Conclusions: In conclusion, through network pharmacology, we have unveiled the underlying mechanisms that link the various components, targets, and pathways influenced by JFAD in the context of lung cancer metastasis. Our experimental results suggest that the oncostatic effects of JFAD may be achieved by upregulating the expression of Lumican/p120ctn and downregulating the levels of specific RhoGTPase family members, which in turn block the PI3K/Akt signaling pathway., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.)
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- 2024
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119. Salvianolic acid F suppresses KRAS-dependent lung cancer cell growth through the PI3K/AKT signaling pathway.
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Hou X, Zhou C, Liang Z, Qiu H, Zhou Z, Zheng H, Li Z, Wang Y, Qi X, Lu L, Cao Y, and Zheng J
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- Humans, Mice, Animals, Proto-Oncogene Proteins c-akt metabolism, Phosphatidylinositol 3-Kinases metabolism, Proto-Oncogene Proteins p21(ras) genetics, Proto-Oncogene Proteins p21(ras) metabolism, Molecular Docking Simulation, Cell Proliferation, Signal Transduction, Cell Transformation, Neoplastic, Mutation, Cell Line, Tumor, Lung pathology, Lung Neoplasms pathology, Carcinoma, Non-Small-Cell Lung metabolism
- Abstract
Background: KRAS mutation is a common driver of NSCLC, and there is a high proportion of lung cancer patients with KRAS G12C and G12D mutation. KRAS was previously considered an "undruggable" target, but the first KRAS G12C mutation-targeted drug AMG510, entered the market in 2021. However, treatments for G12D mutant tumors remain to be discovered. Salvianolic acid F (SalF), a monomer derived from the traditional Chinese medicine Salvia miltiorrhiza (SM), and KRAS had high binding affinity, especially for KRAS G12D. There is an urgent need to investigate effective and safe novel targeted therapies against KRAS G12D-driven NSCLC., Methods: To evaluate the anticancer effect of SalF, we used KRAS-overexpressing lung cancer cells in vitro, a subcutaneous transplant tumor model, and KRAS G12D mice model in vivo. Then, the binding effect of SalF and KRAS was investigated using molecular docking, proteolytic assays and protein thermal shift assays. More critically, the PI3K/AKT signaling pathway in the lung was investigated utilizing RT-qPCR and Western Blotting., Results: This is the first study to evaluate the anticancer effect of SalF on KRAS-overexpressing lung cancer cells or KRAS G12D lung tumors in vivo. We demonstrated that SalF inhibits OE-KRAS A549 cell migration, proliferation and promotes apoptosis in vitro. In addition, we used a subcutaneous transplant tumor model to show that SalF suppresses the growth of lung cancer cells in vivo. Interestingly, our group found that SalF was strongly bound to G12D and could decrease the stability and promoted the degradation of the KRAS G12D mutant through molecular docking, proteolytic assays and protein thermal shift assays. Further research demonstrated that in the Kras
G12D mice model, after SalF treatment, the number and size of mouse lung tumors were significantly reduced. More importantly, SalF can promote apoptosis by inhibiting downstream PI3K/AKT signaling pathway activation., Conclusion: SalF activated apoptosis signaling pathways, suppressed anti-apoptotic genes, and inhibited lung cancer cell growth. These datas suggested that SalF could effectively inhibit the growth of lung tumors with KRAS G12D mutation. SalF may be a novel inhibitor against KRAS G12D, providing a strong theoretical basis for the clinical treatment of lung cancer with KRAS mutations., Competing Interests: Declaration of Competing Interest All authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier GmbH.)- Published
- 2023
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120. Clip-SP1 cleavage activates downstream prophenoloxidase activating protease (PAP) in Plutella xylostella.
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Dong Y, Hou Q, Ye M, Li Z, Li J, You M, Yuchi Z, Lin J, and You S
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- Animals, Larva, Serine Proteases genetics, Serine Proteases metabolism, Enzyme Precursors metabolism, Monophenol Monooxygenase, Insect Proteins metabolism, Serine Endopeptidases genetics, Serine Endopeptidases metabolism, Lepidoptera
- Abstract
Melanization is a component of the humoral immune defense of insects and is induced by serine protease-mediated phenoloxidase (PO) catalysis. Prophenoloxidase (PPO) in the midgut of Plutella xylostella is activated by the CLIP domain serine protease (clip-SP) in response to Bacillus thuringiensis (Bt) infection, but the detailed signaling cascade following this activation is unknown. Here, we report that activation of clip-SP enhances PO activity in the P. xylostella midgut by cleaving three downstream PPO-activating proteases (PAPs). First, the expression level of clip-SP1 was increased in the midgut after Bt8010 infection of P. xylostella. Then, purified recombinant clip-SP1 was able to activate three PAPs - PAPa, PAPb and PAP3 - which in turn enhanced their PO activity in the hemolymph. Furthermore, clip-SP1 showed a dominant effect on PO activity compared to the individual PAPs. Our results indicate that Bt infection induces the expression of clip-SP1, which is upstream of a signaling cascade, to efficiently activate PO catalysis and mediate melanization in the midgut of P. xylostella. And it provides a basis for studying the complex PPO regulatory system in the midgut during Bt infection., (Copyright © 2023 Elsevier Ltd. All rights reserved.)
- Published
- 2023
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121. Clinical diagnostic value of amino acids in laryngeal squamous cell carcinomas.
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Hu S, Zhao C, Wang Z, Li Z, and Kong X
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- Humans, Squamous Cell Carcinoma of Head and Neck diagnosis, Amino Acids, Biomarkers, Tumor, Early Detection of Cancer, Laryngeal Neoplasms diagnosis, Carcinoma, Squamous Cell diagnosis, Head and Neck Neoplasms
- Abstract
Background: Early diagnosis and treatment can improve the survival rates of patients with laryngeal squamous cell carcinoma (LSCC). Therefore, it is necessary to discover new biomarkers for laryngeal cancer screening and early diagnosis., Methods: We collected fasting plasma from LSCC patients and healthy volunteers, as well as cancer and para-carcinoma tissues from LSCC patients, and performed quantitative detection of amino acid levels using liquid chromatography-mass spectrometry. We used overall analysis and multivariate statistical analysis to screen out the statistically significant differential amino acids in the plasma and tissue samples, conducted receiver operating characteristic (ROC) analysis of the differential amino acids to evaluate the sensitivity and specificity of the differential amino acids, and finally determined the diagnostic value of amino acids for laryngeal cancer. Additionally, we identified amino acids in the plasma and tissue samples that are valuable for the early diagnosis of laryngeal cancer classified according to the tumor-node-metastasis (TNM) classification., Results: Asparagine (Asp) and homocysteine (Hcy) were two amino acids of common significance in plasma and tissue samples, and their specificity and sensitivity analysis showed that they may be new biomarkers for the diagnosis and treatment of LSCC. According to the TNM staging system, phenylalanine (Phe) and isoleucine (Ile) were screened out in the plasma of LSCC patients at early (I and II) and advanced (III and IV) stages; ornithine hydrochloride (Orn), glutamic acid (Glu), and Glycine (Gly) were selected in the tissue. These dysregulated amino acids found in LSCC patients may be useful as clinical biomarkers for the early diagnosis and screening of LSCC., Competing Interests: The authors declare that they have no competing interests., (© 2023 Hu et al.)
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- 2023
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122. Theory of Mind Among Swedish Children with ASD, Down Syndrome and Typically Developing Group.
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Shojaeian N, Li Z, Kaurav RPS, and Salem AAMS
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- Child, Humans, Sweden, Autism Spectrum Disorder, Down Syndrome, Theory of Mind
- Abstract
The present study examined the role of IQ and the Theory of Mind understanding in children with an autism spectrum disorder and down syndrome. Sixty-six Swedish children with ASD (n = 26), DS (n = 18), and typically developed group (n = 22) ranged between 6 and 12 years old were compared on ToM tasks consisted of standard ToM and IQ tasks. SPSS 25 program was used to analyze data. The results indicated that individuals with ASD reach a better understanding of first-order ToM tasks than children with DS. This picture was the same in the TD group to show better ability than children with ASD and DS on first-order tasks, except one task which was not found significant differences. To employ second-order TD performed better than clinical groups, while, there was no significant difference between ASD and DS. The scores for the third-order task in children with ASD were significantly better than children with DS., (© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
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- 2022
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123. A GLP-1/glucagon (GCG)/CCK 2 receptors tri-agonist provides new therapy for obesity and diabetes.
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Zhao S, Yan Z, Du Y, Li Z, Tang C, Jing L, Sun L, Yang Q, Tang X, Yuan Y, Han J, and Jiang N
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- Animals, Cholecystokinin, Gastrins agonists, Gastrins therapeutic use, Glucagon-Like Peptide 1 metabolism, Glucagon-Like Peptide-1 Receptor agonists, Hypoglycemic Agents pharmacology, Liraglutide pharmacology, Liraglutide therapeutic use, Mice, Obesity drug therapy, Obesity metabolism, Peptides pharmacology, Receptors, Glucagon agonists, Receptors, Glucagon metabolism, Receptors, Glucagon therapeutic use, Diabetes Mellitus drug therapy, Glucagon
- Abstract
Background and Purpose: Glucagon-like peptide-1 (GLP-1) and glucagon (GCG) receptor dual agonist have promising therapeutic effects in the treatment of obesity and diabetes. Moreover, GLP-1 and cholecystokinin 2 (CCK
2 ) dual agonists have been shown to restore pancreas function and improve glycaemic control in preclinical studies. We describe, for the first time, the beneficial effects of GLP-1/glucagon receptor and GLP-1/CCK2 dual agonists, which can be integrated into one peptide, resulting in significant anti-diabetes and anti-obesity effectiveness., Experimental Approach: The in vitro potency of this novel peptide Xenopus (x) GLP-1/GCG/CCK2 tri-agonist (xGLP/GCG/gastrin) against GLP-1, GCG, CCK1 and CCK2 receptors was determined on cells expressing the corresponding receptors by cAMP accumulation or ERK1/2 phosphorylation assays. The in vivo anti-diabetes and anti-obesity effects of this tri-agonist xGLP/GCG/gastrin were studied in both db/db and diet induced obesity (DIO) mice., Key Results: xGLP/GCG/gastrin was a potent and selective GLP-1, GCG and CCK2 tri-agonist. In DIO mice, the metabolic benefits of xGLP-1/GCG/gastrin, such as reduction of body weight and hepatic lipid contents were significantly better than those of the peptide ZP3022 (GLP-1/CCK-2 dual agonist) and liraglutide. In a short-term study in db/db mice, xGLP/GCG/gastrin treatment had considerable effects, increasing islet numbers, islet areas and insulin content. In a long-term treatment study using db/db mice, xGLP-1/GCG/gastrin showed a significantly and sustained improvement in glucose tolerance and glucose control compared with that of liraglutide, ZP3022, cotadutide (GLP-1/GCG dual agonist) and xGLP/GCG-15., Conclusions and Implications: These results demonstrate the therapeutic potential of xGLP-1/GCG/gastrin for the treatment of obesity and diabetes., (© 2022 British Pharmacological Society.)- Published
- 2022
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124. Quantitative 1 H nuclear magnetic resonance (qHNMR) methods for accurate purity determination of glucosinolates isolated from Isatis indigotica roots.
- Author
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Guo Q, Li Z, Shen L, Xiao Y, and Cheng Z
- Subjects
- Magnetic Resonance Spectroscopy, Protons, Glucosinolates, Isatis
- Abstract
Introduction: Glucosinolates (1-5) are important secondary metabolites found in Isatis indigotica roots. Due to their high hydrophilic and ionic nature, purified glucosinolates often contain salt impurities and moisture. Accurate assessment of their purities is important for glucosinolates being utilised as chemical markers., Objective: To develop and validate quantitative proton (
1 H) nuclear magnetic resonance (qHNMR) methods for purity assessments of aliphatic and indole glucosinolates (1-5)., Method: Several NMR parameters such as pulse program, relaxation time, and delay time were optimised. Three qHNMR methods were developed using gluconapin (3), neoglucobrassicin (4), and sinigrin (5) for method validation and with maleic acid as internal standard., Results: The quantification was based on the integrated area ratios of an olefinic proton (H-4 for 1-3; H-6 for 4; and H-3 for 5) of the side chain from glucosinolates relative to the olefinic proton from the internal standard using deuterated water (D2 O) as the solvent. The qHNMR methods were successfully applied for purity assessments of four aliphatic glucosinolates (1-3 and 5: progoitrin, epiprogoitrin, gluconapin, and sinigrin), and an indole glucosinolate (4: neoglucobrassicin)., Conclusion: The purity of glucosinolates isolated from I. indigotica and commercial sinigrin was accurately assessed using the developed qHNMR method. The qHNMR provides a reliable and superior means to determine the purity of glucosinolates., (© 2020 John Wiley & Sons, Ltd.)- Published
- 2021
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125. Determination of five nucleosides by LC-MS/MS and the application of the method to quantify N 6 -methyladenosine level in liver messenger ribonucleic acid of an acetaminophen-induced hepatotoxicity mouse model.
- Author
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Liu R, Zhao F, Wei J, Yu P, Zhang J, Wang Y, Li Z, Zhang J, Zhang X, and Tian X
- Subjects
- Acetaminophen, Adenosine analogs & derivatives, Animals, Chromatography, High Pressure Liquid, Disease Models, Animal, Male, Mice, Tandem Mass Spectrometry, Adenosine analysis, Chemical and Drug Induced Liver Injury genetics, Cytidine analysis, Guanosine analysis, Liver metabolism, RNA, Messenger chemistry, Uridine analysis
- Abstract
Ribonucleic acid N
6 -methyladenosine methylation plays an important role in a variety of biological processes and diseases. Acetaminophen-induced hepatotoxicity is one of the major challenges faced by clinicians. To date, the link between N6 -methyladenosine and acetaminophen-induced hepatotoxicity has not been studied. In this study, a simple ultra high performance liquid chromatography with tandem mass spectrometry method was developed for the simultaneous determination of five nucleosides (adenosine, uridine, cytidine, guanosine, and N6 -methyladenosine) in messenger ribonucleic acid. After enzymatic digestion of messenger ribonucleic acid, the nucleosides sample was separated on an Acquity UPLC column with gradient elution using methanol and 0.02% formic acid water, and detected by a Qtrap 4500 mass spectrometer with an electrospray ionization mode. The method was validated over the concentration ranges of 4-800 ng/mL for adenosine, uridine, cytidine, and guanosine and 0.1-20 ng/mL for N6 -methyladenosine. It was successfully applied to the determination of N6 -methyladenosine levels in liver messenger ribonucleic acid in an acetaminophen-induced hepatotoxicity mouse model and a control group. This study offers a method for the determination of nucleoside contents in epigenetic studies and constitutes the first step toward the investigation of ribonucleic acid methylation in acetaminophen-induced hepatotoxicity, which will facilitate the elucidation of its mechanism., (© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)- Published
- 2019
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126. 1 H-NMR Based Serum Metabolomics Study to Investigate Hepatoprotective Effect of Qin-Jiao on Carbon Tetrachloride-Induced Acute Hepatotoxicity in Rats.
- Author
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Li Z, Li Y, Lu L, Yang Z, Xue W, Tian X, and Zhang X
- Abstract
Gentiana macrophylla Radix, commonly known as Qin-Jiao (QJ), was recorded alone to treat jaundice in Compendium of Materia Medica and has been frequently prescribed for treatment of liver disease in China. However, the underlying mechanism remains unknown. In the present work, QJ of 1,2 g/kg or silybin of 40 mg/kg (positive control) was orally given to rats for 7 days to verify the protective effect on acute liver damage induced by tetrachloride (CCl
4 ). Together with serum biochemistry and histopathological examination,1 H-NMR based metabolomics work was carried out to investigate the efficacy. It turned out that QJ of 2 g/kg exerted comparable protective effect with positive control and partially recovered disturbed metabolism by CCl4 . Multivariate analysis was conducted and metabolites altered significantly among groups were assigned and discussed, including betaine, glucose, lactate, creatine, and LDL/VLDL. Metabolic regulations involved in QJ or silybin treatment were as follows: tricarboxylic acid (TCA) cycle, synthesis of LDL/VLDL, and gluconeogenesis were enhanced, while betaine metabolism, glycolysis, creatine metabolism, synthesis of ketone bodies, amino acids metabolism, and β -oxidation of fatty acids were suppressed. For the first time hepatoprotective effect of QJ on acute liver damage was revealed by1 H-NMR based metabolomics, prompting understanding of the underlying mechanism.- Published
- 2017
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127. A quantitative ¹H nuclear magnetic resonance (qHNMR) method for assessing the purity of iridoids and secoiridoids.
- Author
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Li Z, Welbeck E, Yang L, He C, Hu H, Song M, Bi K, and Wang Z
- Subjects
- Chromatography, High Pressure Liquid methods, Iridoids chemistry, Molecular Structure, Pyrones chemistry, Reproducibility of Results, Sensitivity and Specificity, Iridoid Glucosides chemistry, Magnetic Resonance Spectroscopy methods
- Abstract
This paper utilized a quantitative (1)H nuclear magnetic resonance (qHNMR) method for assessing the purity of iridoids and secoiridoids. The method was fully validated, including specificity, linearity, accuracy, precision, reproducibility, and robustness. For optimization of experimental conditions, several experimental parameters were investigated, including relaxation delay (D1), scan numbers (NS) and power length (PL1). The quantification was based on the area ratios of H-3 from analytes relative to aromatic protons from 1,4-dinitrobenzene (internal standard) with methanol-d4 as solvent. Five iridoids and secoiridoids (sweroside, swertiamarin, gentiopicroside, geniposide, genipin) were analyzed. Furthermore, the results were validated by the high performance liquid chromatography coupled with ultraviolet detection (HPLC-UV) method. It can be concluded that the qHNMR method was simple, rapid, and accurate, providing a reliable and superior method for assessing the purity of iridoids and secoiridoids., (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Published
- 2015
- Full Text
- View/download PDF
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