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Supplementary Data. Supplementary Table1 Cis-element categories based on biological process. Supplementary Table 2 Primers used in the experiment. Supplementary Table 3 Predicted WAK/WAKL family members in strawberrySupplementary Table 4 Predicted WAK/WAKL family members in apple. Supplementary Table 5 Predicted WAK/WAKL family members in peach. Supplementary Table 6 Number of WAK/WAKL genes in different species. Supplementary Figure 1 Venn diagrams of Genes containing different domains. (a) to (c) represents the quantitative relationships of apple, peach and strawberry respectively. GUB_ WAK_ Bind, galacturonan binding domain, EGF_ CA, calcium binding EGF domain, PKinase, serine/threonine kinase. SignalP&TM, signal peptide and transmembrane helix. Supplementary Figure 2 DNA structures and conserved domains of the WAK/WAKL gene family. Supplementary Figure 3 Paralogous genes pairs in four Rosaceae crops. (a) to (d) represent microsyntenic analysis of apple, peach, rose and strawberry, respectively. The grey lines represent pairs of genes that has syntenic relationship around the genome, and the highlighted red line indicating paralogous pairs ofWAK/WAKL family. Supplementary Figure 4 Syntenic map of orthologous genes running through Rosaceae species. The different coloured bars indicate their chromosomes, the grey lines indicate pairs of genes that are covalently related, and the highlighted red lines mean both of the genes from the WAK/WAKL family. Supplementary Figure 5 Comparison of CDS (coding sequence) of RcWAK2 and RcWAK22. Sequence alignment was performed by DNAMAN with default parameters in pairwise alignment.

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Background: Cistus munbyi Pomel is a well-known medicinal plant in Western Algeria for its several applications in medicine and food. This endemic species from North Africa is poorly studied among the genus of Cistus, which gives the opportunity to carry out original pharmacological studies to value this medicinal plant. Objective: To investigate the antioxidant and the antimicrobial activities of Cistus munbyi, an endemic medicinal plant from Algeria. Methods: The antioxidant properties of C. munbyi crudes extracts were evaluated using the DPPH radical-scavenging activity tests. While their antimicrobial activity was studied by the agar diffusion method and MICs determination against eighteen bacterial and fungal strains, some of which were clinical. Results: The obtained results showed that aqueous and ethanol extracts possess a strong scavenging activity against DPPH radicals with EC50 values estimated at 1.12 and 8.31 μg/mL, respectively. On the other hand, extracts from C. munbyi revealed homogeneous and good antimicrobial activity, in which the inhibition zone diameters observed were ranging from 08 to 19 mm, and the MICs values were included from 0.312 to 5.000 mg/mL. The aqueous extract was less active except against Proteus mirabilis and Staphylococcus aureus. Conclusion: The findings of this study indicate that C. munbyipossesses a strong antioxidant activity and an interesting antimicrobial potential, which allows justifying its uses by the local population in several applications. Therefore, C. munbyi could be a good source of natural antioxidants and antimicrobial agents.