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Due to their traditional use in food fermentation process for centuries, microbial food cultures are considered to have a safe history of use. A specific microbial risk assessment is therefore rarely conducted for fermented foods and their food cultures, inoculated or naturally present. Some of those food cultures have been also considered for their potential health effect as probiotic strain candidates, for which a specific safety demonstration process has been proposed by a joint expert report of FAO and WHO. The European Food Safety Authority (EFSA) Biohazard panel also provides an approach for evaluating the safety of a strain to be added in the food chain, the Qualified Presumption of Safety (QPS). Weissella confusa, former taxon Lactobacillus confusus, is a food culture characterized in the fermentation process of sourdough. Some strains have been recently proposed for their probiotic potential. The species is also documented in recent infection case reports. It is considered nevertheless to be opportunistic as underlying factors have been suggested to explain the infection. We report here the microbial risk assessment of the species, by studying a collection of 26 food and 17 clinical isolates of Weissella confusa. The phenotypic study, genomic characterization and bibliographical survey will allow us to conclude about the safety of the species and confirm its use for food fermentation and consider specific strains for demonstration of their respective health effects as probiotic candidates.

Corrigendum to Characterization of Bifidobacterium species in feaces of the Egyptian fruit bat: Description of Bifidobacterium vespertilionis sp. nov. and Bifidobacterium rousetti sp. nov.

Probiotics are actively used for treatment of diarrhoea, respiratory infections, and prevention of infectious gastrointestinal diseases. The efficacy of probiotics is due to strain-specific features and the number of viable cells; however, several reports of deviations from the label in the actual content of strains in probiotic products are a matter of concern. Most of the available data on quality focuses on probiotic products containing lactobacilli and/or bifidobacteria, while very few data are available on spore-forming probiotics. The present study evaluates the label claims for spore count and species identification in five commercial probiotic products marketed in India and Pakistan that claim to contain Bacillus clausii: Tufpro, Ecogro, Enterogermina, Entromax, and Ospor. Bacterial enumeration from three batches was done by microbiological plating methods by two independent operators. Species identification was done using PCR amplification and sequence analysis of the 16S rRNA gene, and determination of the total amount of species present in the products was done using PCR-denaturing gradient gel electrophoresis (PCR-DGGE) analysis followed by DNA sequencing of the excised bands. Plate count methods demonstrated poor correlations between quantitative label indications and bacteria recovered from plates for Tufpro, Ecogro, and Ospor. The 16S rRNA analysis performed on bacteria isolated from plate counts showed that only Enterogermina and Ospor contained homogenous B. clausii. PCR-DGGE analysis revealed that only Enterogermina had a homogenous B. clausii population while other products had mixed bacterial populations. In conclusion, the current analysis clearly demonstrates that of the five analysed commercial probiotics, only Enterogermina followed the label claims.

Biogas production is a hot topic, which has globally gained interest from many researchers over the past years. This fact is mainly due to the depletion of fossil fuels and environmental concerns regarding wastes disposal. Anaerobic Digestion (AD) represents a biological way to obtain both energy (in form of biogas) and waste discard, by converting the polluting organic matter. The overall process relies on a syntrophic chain where different microbial consortia produce the feed necessary for the final methanogenic step. Cheese whey has been largely investigated for AD treatment, since is a high polluting waste derived from the cheese-making process. However, there is a less-known waste originating from the portioning and shaving phases of long-ripened hard-cheese. This study aimed to investigate the microbiome of anaerobic digesters processing dairy industry wastes, such as cattle manure, cheese whey and hard-cheese powder wastes. In particular, the effects of process parameters, reactor configurations and type of dairy wastes, on the microbial populations, have been analyzed. The goal was achieved by means of culture-independent methods and high throughput sequencing, which allowed quantifying and identifying the main species present, as well as their differential gene expression in relation to hydrogen injection for biogas upgrading purposes.

Plant polyphenols are a broad group of bioactive compounds characterized by different chemical and structural properties, low bioavailability, and several in vitro biological activities. Among these compounds, lignans (a non-flavonoid polyphenolic class found in plant foods for human nutrition) have been recently studied as potential modulators of the gut–brain axis. In particular, gut bacterial metabolism is able to convert dietary lignans into therapeutically relevant polyphenols (i.e., enterolignans), such as enterolactone and enterodiol. Enterolignans are characterized by various biologic activities, including tissue-specific estrogen receptor activation, together with anti-inflammatory and apoptotic effects. However, variation in enterolignans production by the gut microbiota is strictly related to both bioaccessibility and bioavailability of lignans through the entire gastrointestinal tract. Therefore, in this review, we summarized the most important dietary source of lignans, exploring the interesting interplay between gut metabolites, gut microbiota, and the so-called gut–brain axis. Fil: Senizza, Alice. Catholic University Of The Sacred Heart, Piacenza, ; Italia Fil: Rocchetti, Gabriele. Catholic University Of The Sacred Heart, Piacenza, ; Italia Fil: Mosse, Juana Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina Fil: Patrone, Vania. Catholic University Of The Sacred Heart, Piacenza; Italia Fil: Callegari, Maria Luisa. Catholic University Of The Sacred Heart, Piacenza, ; Italia Fil: Morelli, Lorenzo. Catholic University Of The Sacred Heart, Piacenza, ; Italia Fil: Lucini, Luigi. Catholic University Of The Sacred Heart, Piacenza, ; Italia

Sourdough fermentation of bakery products is a well-established and widespread technique to confer an added value to the resulting food. In recent decades, gluten-free raw materials have gained more attention due to the diffusion of food disorders such as coeliac disease, but, at the same time, they present difficult manipulation and scarce technological properties because of the absence of gluten. For this reason, the present work was aimed at selecting starter cultures for sourdough application that are isolated from fermentation of sorghum flour. Three isolates of Lactobacillus fermentum, Weissella cibaria, and Weissella confusa were selected for the following properties: exopolysaccharide synthesis, acidification, CO2 production, and amylase activity. The investigated phenotypic characteristics were confirmed by genomic analyses, which also highlighted other potentially beneficial features for use in bakery products employment. These strains, together with bakery yeast, were used for bread preparation using sorghum and wheat flour and after 24 h of fermentation the resulting dough was analyzed to assess the improvement of its characteristics. The presence of lactic acid bacteria (LAB) had a great impact on the final dough, and the best preparation, from a rheological point of view, resulted in one made of sorghum and wheat flour with added LAB and bakery yeast, whose resulting characteristics were similar to all wheat flour doughs. The results of this study suggest a potential application of the selected starters in sorghum composite bread and should be validated with data from large-scale pilot tests conducted in industrial bakeries.

Background The expansion of renewable energy produced by windmills and photovoltaic panels has generated a considerable electricity surplus, which can be utilized in water electrolysis systems for hydrogen production. The resulting hydrogen can then be funneled to anaerobic digesters for biogas upgrading (biomethanation) purposes (power-to-methane) or to produce high value-added compounds such as short-chain fatty acids (power-to-chemicals). Genome-centric metagenomics and metatranscriptomic analyses were performed to better understand the metabolic dynamics associated with H2 injection in two different configurations of anaerobic digesters treating acidic wastes, specifically cheese manufacturing byproducts. These approaches revealed the key-genes involved in methanation and carbon fixation pathways at species level. Results The biogas upgrading process in the single-stage configuration increased the CH4 content by 7%. The dominant methanogenic species responsible for the upregulation of the hydrogenotrophic pathway in this reactor was Methanothermobacter wolfeii UC0008. In the two-stage configuration, H2 injection induced an upregulation of CO2 fixation pathways producing short-chain fatty acids, mainly acetate and butyrate. In this configuration, the abundant species Anaerobaculum hydrogeniformans UC0046 and Defluviitoga tunisiensis UC0050 primarily upregulated genes related to electron transport chains, suggesting putative syntrophisms with hydrogen scavenger microbes. Interestingly, Tepidanaerobacter acetatoxydans UC0018 did not act as an acetate-oxidizer in either reactor configurations, and instead regulated pathways involved in acetate production and uptake. A putative syntrophic association between Coprothermobacter proteolyticus UC0011 and M. wolfeii UC0008 was proposed in the two-stage reactor. In order to support the transcriptomic findings regarding the hydrogen utilization routes, an advanced bioconversion model was adapted for the simulation of the single- and two-stage reactor setups. Conclusions This is the first study investigating biogas reactor metatranscriptome dynamics following hydrogen injection for biomethanation and carbon fixation to short-chain fatty acids purposes. The same microbes showed different patterns of metabolic regulation in the two reactor configurations. It was observed an effect of the specialized acidogenic reactor on the overall microbial consortium composition and activity in the two-stage digester. There were also suggested the main species responsible for methanation, short-chain fatty acids production, and electron transport chain mechanisms, in both reactor configurations. Electronic supplementary material The online version of this article (10.1186/s40168-018-0583-4) contains supplementary material, which is available to authorized users.

Aims. We study the kinematically distinct components in two early-type galaxies NGC 448 and NGC 4365 aided by integral-field observations with the Multi Unit Spectroscopic Explorer (MUSE) on the Very Large Telescope. We aim to measure the brightness profiles, kinematics, and stellar population properties of the peculiar kinematic structures in these galaxies and shed light on their true nature and formation mechanism. Methods. We use a kinematic decomposition technique to separate the individual contributions to the spectra of the two distinct kinematic components observed at each spatial position in the field of view. By folding back the outcome of a photometric decomposition we reduce the intrinsic degeneracies in recovering the kinematics and the best-fitting stellar spectral templates. Finally, by extracting the Lick line-strength indices for the individual components and fitting them to single stellar population models we derive their ages, metallicities, and {\alpha}/Fe overabundances. Results. The two kinematically decoupled stellar components in NGC 448 have similar ages, but different chemical compositions. The distinct kinematic feature in NGC 448 has a nearly exponential surface-brightness light profile, dominates in the innermost ~ 10", is smaller in size, and is very likely an embedded counter-rotating disk as also indicated by its kinematics. It has higher metallicity than the main galaxy stellar body and lower {\alpha}/Fe overabundance. By contrast, we do not find evidence for true decoupling in the two distinct kinematic components in NGC 4365. This confirms earlier work suggesting that the kinematically distinct core is likely not a separate dynamical structure, but most certainly likely a projection effect stemming from the orbital structure of this galaxy that was previously found to be intrinsically triaxial in shape., Comment: 14 pages, 12 figures, to appear in A&A

We study the kinematics and the stellar populations of the bulge and disc of the spiral galaxy NGC 3521. At each position in the field of view, we separate the contributions of the bulge and the disc from the total observed spectrum and study their kinematics, age, and metallicities independently. Their properties are clearly distinct: the bulge rotates more slowly, has a higher velocity dispersion, and is less luminous than the disc. We identify three main populations of stars in NGC 3521: old ($\geq7$ Gyr), intermediate ($\approx$ 3 Gyr), and young ($\leq$1 Gyr). The mass and light of NGC 3521 are dominated by the intermediate stellar population. The youngest population contributes mostly to the disc component and its contribution increases with radius. We also study the luminosity-weighed properties of the stars in NGC 3521. Along the photometric major axis, we find: i) no age gradient for the stars in the bulge, and a negative age gradient for the stars in the disc; ii) negative metallicity gradients and sub-solar $\alpha$-enhancement for both the bulge and the disc. We propose the following picture for the formation of NGC 3521: initial formation a long time ago ($\geq 7$ Gyr), followed by a second burst of star formation or a merger ($\approx$ 3 Gyrs ago), which contributed predominantly to the mass-build up of the bulge. Recently ($\leq 1$ Gyr), the disc of NGC 3521 experienced an additional episode of star formation that started in the innermost regions., Comment: 13 pages, 11 figures, accepted for publication in MNRAS

Four novel Gram-stain-positive, non spore forming and fructose-6-phosphate phosphoketolase-positive strains were isolated from the faeces of a cotton top tamarin (Saguinus oedipus) and an emperor tamarin (Saguinus imperator). Phylogenetic analyses based on 16S rRNA revealed that bifidobacterial strains TRE 1T exhibit close phylogenetic relatedness to Bifidobacterium catulorum DSM 103154 (96.0%) and Bifidobacterium tissieri DSM 100201 (96.0%); TRE DT and TRE HT were closely related to Bifidobacterium longum subsp. longum ATCC 15708T with similarity values of 97.4% and 97.5%, respectively; TRI 7T was closely related to Bifidobacterium tissieri DSM 100201 (96.0%). The Average Nucleotide Identity (ANI) and in silico DDH (isDDH) analysis with closest neighbour supported an independent phylogenetic position of all strains with values ranged from 74 to 85% for ANI and from 24 to 28% for isDDH. DNA base composition of the four strains was in the range of 58.3–63.5 mol% G + C. Based on the phylogenetic, genotypic and phenotypic data, the strains TRE 1T, TRE DT, TRE HT and TRI 7T clearly represent four novel taxa within the genus Bifidobacterium for which the names Bifidobacterium primatium sp. nov. (type strain TRE 1T = DSM 100687T = JCM 30945T), Bifidobacterium scaligerum sp. nov. (type strain TRE DT = DSM 103140T = JCM 31792T), Bifidobacterium felsineum sp. nov. (type strain TRE HT = DSM 103139T = JCM 31789T) and Bifidobacterium simiarum sp. nov. (type strain TRI 7T = DSM 103153T = JCM 31793) are proposed.

The present research is the first comprehensive study regarding the thermophilic anaerobic degradation of cheese wastewater, which combines the evaluation of different reactor configurations (i.e. single and two-stage continuous stirred tank reactors) on the process efficiency and the in-depth characterization of the microbial community structure using genome-centric metagenomics. Both reactor configurations showed acidification problems under the tested organic loading rates (OLRs) of 3.6 and 2.4 g COD/L-reactor day and the hydraulic retention time (HRT) of 15 days. However, the two-stage design reached a methane yield equal to 95% of the theoretical value, in contrast with the single stage configuration, which reached a maximum of 33% of the theoretical methane yield. The metagenomic analysis identified 22 new population genomes and revealed that the microbial compositions between the two configurations were remarkably different, demonstrating a higher methanogenic biodiversity in the two-stage configuration. In fact, the acidogenic reactor of the serial configuration was almost solely composed by the lactose degrader Bifidobacterium crudilactis UC0001. The predictive functional analyses of the main population genomes highlighted specific metabolic pathways responsible for the AD process and the mechanisms of main intermediates production. Particularly, the acetate accumulation experienced by the single stage configuration was mainly correlated to the low abundant syntrophic acetate oxidizer Tepidanaerobacter acetatoxydans UC0018 and to the absence of aceticlastic methanogens.

Fossil groups (FGs) are galaxy aggregates with an extended and luminous X-ray halo, which are dominated by a very massive early-type galaxy and lack of L* objects. FGs are indeed characterized by a large magnitude gap between their central and surrounding galaxies. This is explained by either speculating that FGs are failed groups that formed without bright satellite galaxies and did not suffer any major merger, or by suggesting that FGs are very old systems that had enough time to exhaust their bright satellite galaxies through multiple major mergers. Since major mergers leave signatures in the stellar populations of the resulting galaxy, we study the stellar population parameters of the brightest central galaxies (BCGs) of FGs as a benchmark against which the formation and evolution scenarios of FGs can be compared. We present long-slit spectroscopic observations along different axes of NGC 6482 and NGC 7556, which are the BCGs of two nearby FGs. The measurements include spatially resolved stellar kinematics and radial profiles of line-strength indices, which we converted into stellar population parameters using single stellar-population models. NGC 6482 and NGC 7556 are very massive and large galaxies and host a centrally concentrated stellar population, which is significantly younger and more metal rich than the rest of the galaxy. The age gradients of both galaxies are somewhat larger than those of the other FG BCGs studied so far, whereas their metallicity gradients are similarly negative and shallow. They have negligible gradients of alpha-element abundance ratio. The measured metallicity gradients are less steep than those predicted for massive galaxies that formed monolithically and evolved without experiencing any major merger. We conclude that the observed FGs formed through major mergers rather than being failed groups that lacked bright satellite galaxies from the beginning., Comment: 17 pages, 9 figures, A&A in press, corrected proofs

The chapter begins with an overview of the definitions of the three terms presented in the title, from both a scientific and a regulatory point of view. Then the clinical evidences available on the beneficial effects obtained by dosing selected strains of bifidobacteria or feeding human subjects with prebiotic substances will be addressed, taking into consideration different stages in aging (neonatal life, adulthood, and elders in their “ golden years”). A section will be devoted to “therapeutic” use of bifidobacteria, that is, administration to subjects in pathological conditions. A final section will be devoted to an update on the mechanisms of action of probiotic bifidobacteria, as well as the impact of prebiotics studies by means of microbiome analysis.

Flattened bulges with disk-like properties are considered to be the end product of secular evolution processes at work in the inner regions of galaxies. On the contrary, classical bulges are characterized by rounder shapes and thought to be similar to low-luminosity elliptical galaxies. We aim at testing the variety of observational diagnostics which are commonly adopted to separate classical from disk-like bulges in nearby galaxies. We select a sample of eight unbarred lenticular galaxies to be morphologically and kinematically undisturbed with no evidence of other components than bulge and disk. We analyze archival data of broad-band imaging from SDSS and integral-field spectroscopy from the ATLAS$^{\rm 3D}$ survey to derive the photometric and kinematic properties, line-strength indices, and intrinsic shape of the sample bulges. We argue that the bulge S\'ersic index is a poor diagnostics to discriminate different bulge types. We find that the combination of line-strength with either kinematic or photometric diagnostics does not provide a clear separation for half of the sample bulges. We include for the first time the intrinsic three-dimensional shape of bulges as a possible discriminant of their nature. All bulges turn out to be thick oblate spheroids, but only one has a flattening consistent with that expected for outer disks. We conclude that bulge classification may be difficult even adopting all observational diagnostics proposed so far and that classical and disk-like bulges could be more confidently identified by considering their intrinsic shape.

The formation scenario of extended counter-rotating stellar disks in galaxies is still debated. In this paper, we study the S0 galaxy IC 719 known to host two large-scale counter-rotating stellar disks in order to investigate their formation mechanism. We exploit the large field of view and wavelength coverage of the Multi Unit Spectroscopic Explorer (MUSE) spectrograph to derive two-dimensional (2D) maps of the various properties of the counter-rotating stellar disks, such as age, metallicity, kinematics, spatial distribution, the kinematical and chemical properties of the ionized gas, and the dust map. Due to the large wavelength range, and in particular to the presence of the Calcium Triplet 8498, 8542, 8662\AA (CaT hereafter), the spectroscopic analysis allows us to separate the two stellar components in great detail. This permits precise measurement of both the velocity and velocity dispersion of the two components as well as their spatial distribution. We derived a 2D map of the age and metallicity of the two stellar components as well as the star formation rate and gas-phase metallicity from the ionized gas emission maps. The main stellar disk of the galaxy is kinematically hotter, older, thicker and with larger scale-length than the secondary disk. There is no doubt that the latter is strongly linked to the ionized gas component: they have the same kinematics and similar vertical and radial spatial distribution. This result is in favor of a gas accretion scenario over a binary merger scenario to explain the origin of counter-rotation in IC 719. One source of gas that may have contributed to the accretion process is the cloud that surrounds IC 719., Comment: 13 pages, 20 figures, accepted for publication in A&A

Natural casings derived from different intestine portions have been used for centuries in the production of fresh and dry-fermented sausages. Here we analysed by means of culture-dependent methods and Illumina high-throughput sequencing of 16S rRNA amplicons the bacterial ecology of hog, cow and ovine casings at different stages of their preparation for sausages production. Several strains of Staphylococcus , Lactobacillus , Bifidobacterium , Vagococcus and Clostridium were counted, isolated and characterised at phylogenetic level. High-throughput sequencing analyses revealed a high bacterial diversity, which differed strongly between casings of different animal species. The technological processes involved in the preparation for casing had also a strong impact on the casings bacterial ecology, with a significant reduction of undesired microorganisms, and an increase in the proportion of lactobacilli and staphylococci. Natural casings were demonstrated to be complex ecological environments, whose role as microbiological inoculants in the production of sausages should not be underestimated.

Growing evidence underlines the pivotal role of infant gut colonization in the development of the immune system. The possibility to modify gut colonization through probiotic supplementation in childhood might prevent atopic diseases. The aim of the present systematic review and meta-analysis was to evaluate the effect of probiotic supplementation during pregnancy and early infancy in preventing atopic diseases. PubMed, Embase and Cochrane Library were searched for randomized controlled trials evaluating the use of probiotics during pregnancy or early infancy for prevention of allergic diseases. Fixed-effect models were used, and random-effects models where significant heterogeneity was present. Results were expressed as risk ratio (RR) with 95% confidence interval (CI). Seventeen studies, reporting data from 4755 children (2381 in the probiotic group and 2374 in the control group), were included in the meta-analysis. Infants treated with probiotics had a significantly lower RR for eczema compared to controls (RR 0.78 [95% CI: 0.69-0.89], P = 0.0003), especially those supplemented with a mixture of probiotics (RR 0.54 [95% CI: 0.43-0.68], P

We investigated the properties of the stellar populations in the discs of a sample of ten spiral galaxies. Our analysis focused on the galaxy region where the disc contributes more than 95 per cent of total surface brightness in order to minimise the contamination of the bulge and bar. The luminosity-weighted age and metallicity were obtained by fitting the galaxy spectra with a linear combination of stellar population synthesis models, while the total overabundance of {\alpha}-elements over iron was derived by measuring the line-strength indices. Most of the sample discs display a bimodal age distribution and they are characterised by a total [{\alpha}/Fe] enhancement ranging from solar and supersolar. We interpreted the age bimodality as due to the simultaneous presence of both a young (Age$\,\leq\,4$ Gyr) and an old (Age$\,>\,$4 Gyr) stellar population. The old stellar component usually dominates the disc surface brightness and its light contribution is almost constant within the observed radial range. For this reason, no age gradient is observed in half of the sample galaxies. The old component is slightly more metal poor than the young one. The metallicity gradient is negative and slightly positive in the old and young components, respectively. These results are in agreement with an inside-out scenario of disc formation and suggest a reduced impact of the radial migration on the stellar populations of the disc. The young component could be the result of a second burst of star formation in gas captured from the environment., Comment: 13 pages 15 figures and 6 tables, MNRAS in press

The bacterial diversity involved in food fermentations is one of the most important factors shaping the final characteristics of traditional foods. Knowledge about this diversity can be greatly improved by the application of high-throughput sequencing technologies (HTS) coupled to the PCR amplification of the 16S rRNA subunit. Here we investigated the bacterial diversity in batches of Salame Piacentino PDO (Protected Designation of Origin), a dry fermented sausage that is typical of a regional area of Northern Italy. Salami samples from 6 different local factories were analysed at 0, 21, 49 and 63 days of ripening; raw meat at time 0 and casing samples at 21 days of ripening where also analysed, and the effect of starter addition was included in the experimental set-up. Culture-based microbiological analyses and PCR-DGGE were carried out in order to be compared with HTS results. A total of 722,196 high quality sequences were obtained after trimming, paired-reads assembly and quality screening of raw reads obtained by Illumina MiSeq sequencing of the two bacterial 16S hypervariable regions V3 and V4; manual curation of 16S database allowed a correct taxonomical classification at the species for 99.5% of these reads. Results confirmed the presence of main bacterial species involved in the fermentation of salami as assessed by PCR-DGGE, but with a greater extent of resolution and quantitative assessments that are not possible by the mere analyses of gel banding patterns. Thirty-two different Staphylococcus and 33 Lactobacillus species where identified in the salami from different producers, while the whole data set obtained accounted for 13 main families and 98 rare ones, 23 of which were present in at least 10% of the investigated samples, with casings being the major sources of the observed diversity. Multivariate analyses also showed that batches from 6 local producers tend to cluster altogether after 21 days of ripening, thus indicating that HTS has the potential for fine scale differentiation of local fermented foods.

Key information to understand the formation and evolution of disk galaxies are imprinted in the stellar populations of their bulges. This paper has the purpose to make available new measurements of the stellar population properties of the bulges of four spiral galaxies. Both the central values and radial profiles of the line strength of some of the most common Lick indices are measured along the major- and minor- axis of the bulge-dominated region of the sample galaxies. The corresponding age, metallicity, and α /Fe ratio are derived by using the simple stellar population synthesis model predictions. The central values and the gradients of the stellar population properties of ESO-LV 1890070, ESO-LV 4460170, and ESO-LV 5140100 are consistent with previous findings for bulges of spiral galaxies. On the contrary, the bulge of ESO-LV 4500200 shows peculiar chemical properties possibly due to the presence of a central kinematically-decoupled component. The negative metallicity gradient found in our bulges sample indicates a relevant role for the dissipative collapse in bulge formation. However, the shallow gradients found for the age and α /Fe ratio suggests that merging can not be completely ruled out for the sample bulges. This is confirmed by the properties of ESO-LV 4500200 which can hardly be explained without invoking the capture of external material. (© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

This study aimed to systematically evaluate safety of probiotics and synbiotics in immune compromised adults (≥18 years). Safety was analysed using the Common Terminology Clinical Adverse Events (CTCAE version 4.0) classification, thereby providing an update on previous reports using the most recent available clinical data (2008-2013). Safety aspects are represented and related to number of participants per probiotic strain/culture, study duration, dosage, clinical condition and selected afflictions. Analysis of 57 clinical studies indicates that probiotic and/or synbiotic administration in immune compromised adults is safe with regard to the current evaluated probiotic strains, dosages and duration. Individuals were considered immune compromised if HIV-infected, critically ill, underwent surgery or had an organ- or an autoimmune disease. There were no major safety concerns in the study, as none of the serious adverse events (AE)s were related, or suspected to be related, to the probiotic or synbiotic product and the study products were well tolerated. Overall, AEs occurred less frequent in immune compromised subjects receiving probiotics and/or synbiotics compared to the control group. In addition, the results demonstrated a flaw in precise reporting and classification of AE in most studies. Furthermore, generalisability of conclusions are greatly limited by the inconsistent, imprecise and potentially incomplete reporting as well as the variation in probiotic strains, dosages, administration regimes, study populations and reported outcomes. We argue that standardised reporting on adverse events (CTCAE) in ‘food’ studies should be obligatory, thereby improving reliability of data and re-enforcing the safety profile of probiotics.

This study aimed to systematically evaluate safety of probiotics and synbiotics in children ageing 0-18 years. This study is the third and final part in a safety trilogy and an update is provided using the most recent available clinical data (2008-2013) by means of the Common Terminology Clinical Adverse Events (CTCAE version 4.0) classification. Safety aspects are represented and related to number of participants per probiotic strain/culture, study duration, dosage, clinical condition and selected afflictions. Analysis of 74 clinical studies indicated that probiotic and/or synbiotic administration in children is safe with regard to the specific evaluated strains, dosages and duration. The population of children include healthy, immune compromised and obese subjects, as well as subjects with intestinal disorders, infections and inflammatory disorders. This study revealed no major safety concerns, as the adverse events (AEs) were unrelated, or not suspected to be related, to the probiotic or synbiotic product. In general the study products were well tolerated. Overall, AEs occurred more frequent in the control arm compared to children receiving probiotics and/or synbiotics. Furthermore, the results indicate inadequate reporting and classification of AEs in the majority of the studies. In addition, generalizability of conclusions are greatly limited by the inconsistent, imprecise and potentially incomplete reporting as well as the variation in probiotic strains, dosages, administration regimes, study populations and reported outcomes.

Antibiotic associated diarrhea (AAD) is a common complication in childhood in the outpatient and inpatient settings. This review provides up to date information on the use of probiotics in the prevention and treatment of AAD, including that from Clostridium Difficile, in children. The most recently systematic reviews and subsequently published randomized controlleds trials are considered. Different single and multistrain probiotics are described; a specific recommendation for the use of Lactobacillus Rhamnosus GG (LGG) and Saccharomyces boulardii (Sb) emerges. New information on LGG survival under amoxicillin/clavulanate therapy in children is also provided. This information is relevant in view of the frequent use of this molecule in children, its association with AAD, and LGG's sensitivity to penicillin that might make this probiotic ineffective. In spite of a demonstrated positive effect of specific strains of probiotics on AAD, safety issues still remain among which the risk of associated severe infections and of antibiotic resistant gene exchange.

We present new stringent limits on the mass $M_{bh}$ of the central supermassive black hole for a sample of 7 nearby galaxies. Our $M_{bh}$ estimates are based on the dynamical modeling of the central width of the nebular emission lines measured over subarcsecond apertures with the Hubble Space Telescope. The central stellar velocity dispersion $\sigma_c$ of the sample galaxies is derived from new long-slit spectra from ground-based observations and the bulge effective radius is obtained from a two-dimensional photometric decomposition of the i-band images from the Sloan Digital Sky Survey. The derived stringent $M_{bh}$ limits run parallel and above the $M_{bh}-\sigma_c$ relation with no systematic trend depending on the galaxy distance or morphology. This gives further support to previous findings suggesting that the nuclear gravitational potential is remarkably well traced by the width of the nebular lines when the gas is centrally peaked. With our investigation, the number of galaxies with stringent $M_{bh}$ limits obtained from nebular-line width increases to 114 and can be used for studying the scaling relations between $M_{bh}$ and properties of their host galaxies., Comment: 13 pages, 8 figures, AN in press

Context. Many disk galaxies host two extended stellar components that rotate in opposite directions. The analysis of the stellar populations of the counter-rotating components provides constraints on the environmental and internal processes that drive their formation. Aims. The S0 NGC 1366 in the Fornax cluster is known to host a stellar component that is kinematically decoupled from the main body of the galaxy. Here we successfully separated the two counter-rotating stellar components to independently measure the kinematics and properties of their stellar populations. Methods. We performed a spectroscopic decomposition of the spectrum obtained along the galaxy major axis and separated the relative contribution of the two counter-rotating stellar components and of the ionized-gas component. We measured the line-strength indices of the two counter-rotating stellar components and modeled each of them with single stellar population models that account for the {\alpha}/Fe overabundance. Results. We found that the counter-rotating stellar component is younger, has nearly the same metallicity, and is less {\alpha}/Fe enhanced than the corotating component. Unlike most of the counter-rotating galaxies, the ionized gas detected in NGC 1366 is neither associated with the counter-rotating stellar component nor with the main galaxy body. On the contrary, it has a disordered distribution and a disturbed kinematics with multiple velocity components observed along the minor axis of the galaxy. Conclusions. The different properties of the counter-rotating stellar components and the kinematic peculiarities of the ionized gas suggest that NGC 1366 is at an intermediate stage of the acquisition process, building the counter-rotating components with some gas clouds still falling onto the galaxy., Comment: A&A in press. 8 pages, 6 figures, 2 Table

Bacteria used to ferment milk to obtain yogurt belong to thermophilic, bile-sensitive species of lactic acid bacteria, not ideally suited for survival in the human gut. A significant part of the available scientific literature reports positive effects on human health because of the action of yogurt starter cultures, without any reference to a strain-specific action. On the contrary, literature dealing with beneficial effects of probiotic bacteria of intestinal origin strongly supports a strain-specific activity. In this chapter, available information on the potential of a strain-specific beneficial action as well as on yogurt starter cultures is reviewed.

Context.The intrinsic shape of galactic bulges in nearby galaxies provides crucial information to separate bulge types.Aims.We aim to derive accurate constraints to the intrinsic shape of bulges to provide new clues on their formation mechanisms and set new limitations for future simulations.Methods.We retrieved the intrinsic shape of a sample of CALIFA bulges using a statistical approach. Taking advantage of GalMer numerical simulations of binary mergers we estimated the reliability of the procedure. Analyzing thei-band mock images of resulting lenticular remnants, we studied the intrinsic shape of their bulges at different galaxy inclinations. Finally, we introduced a new (B/A,C/A) diagram to analyze possible correlations between the intrinsic shape and the properties of bulges.Results.We tested the method on simulated lenticular remnants, finding that for galaxies with inclinations of 25° ≤θ≤ 65° we can safely derive the intrinsic shape of their bulges. We found that our CALIFA bulges tend to be nearly oblate systems (66%), with a smaller fraction of prolate spheroids (19%), and triaxial ellipsoids (15%). The majority of triaxial bulges are in barred galaxies (75%). Moreover, we found that bulges with low Sérsic indices or in galaxies with low bulge-to-total luminosity ratios form a heterogeneous class of objects; additionally, bulges in late-type galaxies or in less massive galaxies have no preference for being oblate, prolate, or triaxial. On the contrary, bulges with high Sérsic index, in early-type galaxies, or in more massive galaxies are mostly oblate systems.Conclusions.We concluded that various evolutionary pathways may coexist in galaxies, with merging events and dissipative collapse being the main mechanisms driving the formation of the most massive oblate bulges and bar evolution reshaping the less massive triaxial bulges.

Growing evidence supports the role of probiotics in reducing the risk of necrotizing enterocolitis, time to achieve full enteral feeding, and late-onset sepsis (LOS) in preterm infants. As reported for several neonatal clinical outcomes, recent data have suggested that nutrition might affect probiotics’ efficacy. Nevertheless, the currently available literature does not explore the relationship between LOS prevention and type of feeding in preterm infants receiving probiotics. Thus, the aim of this systematic review and meta-analysis was to evaluate the effect of probiotics for LOS prevention in preterm infants according to type of feeding (exclusive human milk (HM) vs. exclusive formula or mixed feeding). Randomized-controlled trials involving preterm infants receiving probiotics and reporting on LOS were included in the systematic review. Only trials reporting on outcome according to feeding type were included in the meta-analysis. Fixed-effects models were used and random-effects models were used when significant heterogeneity was found. The results were expressed as risk ratio (RR) with 95% confidence interval (CI). Twenty-five studies were included in the meta-analysis. Overall, probiotic supplementation resulted in a significantly lower incidence of LOS (RR 0.79 (95% CI 0.71–0.88), p < 0.0001). According to feeding type, the beneficial effect of probiotics was confirmed only in exclusively HM-fed preterm infants (RR 0.75 (95% CI 0.65–0.86), p < 0.0001). Among HM-fed infants, only probiotic mixtures, and not single-strain products, were effective in reducing LOS incidence (RR 0.68 (95% CI 0.57–0.80) p < 0.00001). The results of the present meta-analysis show that probiotics reduce LOS incidence in exclusively HM-fed preterm infants. Further efforts are required to clarify the relationship between probiotics supplementation, HM, and feeding practices in preterm infants.

A minimum in stellar velocity dispersion is often observed in the central regions of disc galaxies. To investigate the origin of this feature, known as a {\sigma}-drop, we analyse the stellar kinematics of a high-resolution N-body + smooth particle hydrodynamical simulation, which models the secular evolution of an unbarred disc galaxy. We compared the intrinsic mass-weighted kinematics to the recovered luminosity-weighted ones. The latter were obtained by analysing synthetic spectra produced by a new code, SYNTRA, that generates synthetic spectra by assigning a stellar population synthesis model to each star particle based on its age and metallicity. The kinematics were derived from the synthetic spectra as in real spectra to mimic the kinematic analysis of real galaxies. We found that the recovered luminosity-weighted kinematics in the centre of the simulated galaxy are biased to higher rotation velocities and lower velocity dispersions due to the presence of young stars in a thin and kinematically cool disc, and are ultimately responsible for the {\sigma}-drop., Comment: 9 pages, 7 figures, published on MNRAS

Recent evidence suggests that there is a link between the gut microbial community and immune-mediated disorders. Systemic sclerosis (SSc) is an autoimmune disease characterized by immunonological abnormalities, vascular lesions, and extensive fibrosis. Since the gastrointestinal tract is one of the organs most involved, the goal of this study was to explore the composition of the intestinal microbiota in SSc patients with (SSc/GI+) and without gastrointestinal involvement (SSc/GI-) in comparison to healthy controls (HC). The fecal bacterial composition was investigated by Illumina sequencing of 16 S rRNA gene amplicons. The fecal microbiota of SSc/GI+ subjects was characterized by higher levels of Lactobacillus, Eubacterium and Acinetobacter compared with healthy controls, and lower proportions of Roseburia, Clostridium, and Ruminococcus. The gut microbiota of SSc/GI- subjects was more similar to the microbiota of HC than to that of SSc/GI+ subjects albeit Streptococcus salivarius was over-represented in SSc/GI- fecal samples compared with both SSc/GI+ subjects and controls. Our study reveals microbial signatures of dysbiosis in the gut microbiota of SSc patients that are associated with clinical evidence of gastrointestinal disease. Further studies are needed to elucidate the potential role of these perturbations in the onset and progression of systemic sclerosis, and gastrointestinal involvement in particular.

We present the results of an optical spectroscopic monitoring program targeting NGC 5548 as part of a larger multi-wavelength reverberation mapping campaign. The campaign spanned six months and achieved an almost daily cadence with observations from five ground-based telescopes. The H$\beta$ and He II $\lambda$4686 broad emission-line light curves lag that of the 5100 $\AA$ optical continuum by $4.17^{+0.36}_{-0.36}$ days and $0.79^{+0.35}_{-0.34}$ days, respectively. The H$\beta$ lag relative to the 1158 $\AA$ ultraviolet continuum light curve measured by the Hubble Space Telescope is roughly $\sim$50% longer than that measured against the optical continuum, and the lag difference is consistent with the observed lag between the optical and ultraviolet continua. This suggests that the characteristic radius of the broad-line region is $\sim$50% larger than the value inferred from optical data alone. We also measured velocity-resolved emission-line lags for H$\beta$ and found a complex velocity-lag structure with shorter lags in the line wings, indicative of a broad-line region dominated by Keplerian motion. The responses of both the H$\beta$ and He II $\lambda$4686 emission lines to the driving continuum changed significantly halfway through the campaign, a phenomenon also observed for C IV, Ly $\alpha$, He II(+O III]), and Si IV(+O IV]) during the same monitoring period. Finally, given the optical luminosity of NGC 5548 during our campaign, the measured H$\beta$ lag is a factor of five shorter than the expected value implied by the $R_\mathrm{BLR} - L_\mathrm{AGN}$ relation based on the past behavior of NGC 5548., Comment: 22 pages, 13 figures, accepted to ApJ

We present the nature of 50 hard X-ray emitting objects unveiled through an optical spectroscopy campaign performed at seven telescopes in the northern and southern hemispheres. These objects were detected with the Burst Alert Telescope (BAT) instrument onboard the Swift satellite and listed as of unidentified nature in the 54-month Palermo BAT catalogue. In detail, 45 sources in our sample are identified as active galactic nuclei of which, 27 are classified as type 1 (with broad and narrow emission lines) and 18 are classified as type 2 (with only narrow emission lines). Among the broad-line emission objects, one is a type 1 high-redshift quasi-stellar object, and among the narrow-line emission objects, one is a starburst galaxy, one is a X-ray bright optically normal galaxy, and one is a low ionization nuclear emission line region. We report 30 new redshift measurements, 13 confirmations and 2 more accurate redshift values. The remaining five objects are galactic sources: three are Cataclismic Variables, one is a X-ray Binary probably with a low mass secondary star, and one is an active star. Based on observations obtained from the following observatories: Cerro Tololo Interamerican Observatory (Chile); Astronomical Observatory of Bologna in Loiano (Italy); Observatorio Astronómico Nacional (San Pedro Mártir, Mexico); Radcliffe telescope of the South African Astronomical Observatory (Sutherland, South Africa); Sloan Digital Sky Survey; Observatorio del Roque de los Muchachos of the Instituto de Astrofísica de Canarias (Canary Islands, Spain) and New Technology Telescope (NTT) of La Silla Observatory, Chile.

Delivery mode (natural vs. cesarean) and feeding type (breast vs. formula feeding) are relevant factors for neonatal gut colonization. Biomolecular methods have shown that the ecological structure of infant microbiota is more complex than previously proposed, suggesting a relevant presence of unculturable bacteria. It has also been postulated that among unculturable bacteria, hydrogenotrophic populations might play a key role in infant health. Sulfate-reducing bacteria (SRB), acetogens, and methanogenic archaea use hydrogenotrophic pathways within the human colon. However, to date, few studies have reported detection of hydrogenotrophic microorganisms in newborns, possibly because of limitations on available group-specific, culture-independent quantification procedures. In the present work, we analyzed 16 fecal samples of healthy babies aged 1 to 6 months by means of quantitative PCR (qPCR) targeting the 16S rRNA or metabolic functional genes and by denaturing gradient gel electrophoresis (DGGE). qPCR data showed quantifiable levels of methanogens, SRB and acetogens in all samples, indicating that the relative abundances of these microbial groups were not affected by delivery mode (natural vs. caesarian). DGGE revealed a high prevalence of the Blautia genus within the acetogenic bacteria despite strong inter-individual variability. Our preliminary results suggest that hydrogenotrophic microorganisms, which have been a neglected group to date, should be included in future ecological and metabolic studies evaluating the infant intestinal microbiota.

We present a spectral decomposition technique that separates the contribution of different kinematic components in galaxies from the observed spectrum. This allows to study the kinematics and properties of the stellar populations of the individual components (e.g., bulge, disk, counter-rotating cores, orthogonal structures). Here, we discuss the results of this technique for galaxies that host counter-rotating stellar disks of comparable size. In all the studied cases, the counter-rotating stellar disk is the less massive, the youngest and has different chemical content (metallicity and alpha-elements abundance ratio) than the main galaxy disk. Further applications of the spectral decomposition technique are also discussed., Comment: 4 Pages, 3 figures. Coccato et al. 2014, in IAU Symp. 309, "Galaxies in 3D across the Universe", B. L. Ziegler, F. Combes, H. Dannerbauer, M. Verdugo, Eds. (Cambridge: Cambridge Univ. Press), in press

An expert panel was convened in October 2013 by the International Scientific Association for Probiotics and Prebiotics (ISAPP) to discuss the field of probiotics. It is now 13 years since the definition of probiotics and 12 years after guidelines were published for regulators, scientists and industry by the Food and Agriculture Organization of the United Nations and the WHO (FAO/WHO). The FAO/WHO definition of a probiotic--"live microorganisms which when administered in adequate amounts confer a health benefit on the host"--was reinforced as relevant and sufficiently accommodating for current and anticipated applications. However, inconsistencies between the FAO/WHO Expert Consultation Report and the FAO/WHO Guidelines were clarified to take into account advances in science and applications. A more precise use of the term 'probiotic' will be useful to guide clinicians and consumers in differentiating the diverse products on the market. This document represents the conclusions of the ISAPP consensus meeting on the appropriate use and scope of the term probiotic.

Empirical stellar spectral libraries have applications in both extragalactic and stellar studies, and they have an advantage over theoretical libraries because they naturally include all relevant chemical species and physical processes. During recent years we see a stream of new high quality sets of spectra, but increasing the spectral resolution and widening the wavelength coverage means resorting to multi-order echelle spectrographs. Assembling the spectra from many pieces results in lower fidelity of their shapes. We aim to offer the community a library of high signal-to-noise spectra with reliable continuum shapes. Furthermore, the using an integral field unit (IFU) alleviates the issue of slit losses. Our library was build with the MUSE (Multi-Unit Spectroscopic Explorer) IFU instrument. We obtained spectra over nearly the entire visual band (lambda~4800-9300 Ang). We assembled a library of 35 high-quality MUSE spectra for a subset of the stars from the X-shooter Spectral Library. We verified the continuum shape of these spectra with synthetic broad band colors derived from the spectra. We also report some spectral indices from the Lick system, derived from the new observations. We offer a high-fidelity set of stellar spectra that covers the Hertzsprung-Russell diagram. It can be useful for both extragalactic and stellar work and demonstrates that the IFUs are excellent tools for building reliable spectral libraries., accepted in A&A; 11 pages, 7 figures (some panels are omitted in the figures in the appendix to reduce the file size)

We present photometry and long-slit spectroscopy for 12 S0 and spiral galaxies selected from the Catalogue of Isolated Galaxies. The structural parameters of the sample galaxies are derived from the Sloan Digital Sky Survey i-band images by performing a two-dimensional photometric decomposition of the surface brightness distribution. This is assumed to be the sum of the contribution of a S\`ersic bulge, an exponential disc, and a Ferrers bar characterized by elliptical and concentric isophotes with constant ellipticity and position angles. The rotation curves and velocity dispersion profiles of the stellar component are measured from the spectra obtained along the major axis of galaxies. The radial profiles of the H{\beta}, Mg and Fe line-strength indices are derived too. Correlations between the central values of the Mg 2 and Fe line-strength indices and the velocity dispersion are found. The mean age, total metallicity and total {\alpha}/Fe enhancement of the stellar population in the centre and at the radius where the bulge gives the same contribution to the total surface brightness as the remaining components are obtained using stellar population models with variable element abundance ratios. We identify intermediate-age bulges with solar metallicity and old bulges with a large spread in metallicity. Most of the sample bulges display super-solar {\alpha}/Fe enhancement, no gradient in age and negative gradients of metallicity and {\alpha}/Fe enhancement. These findings support a formation scenario via dissipative collapse where environmental effects are remarkably less important than in the assembly of bulges of galaxies in groups and clusters., Comment: MNRAS in press. 29 pages, 12 figures, 10 Table. Figure A2 have lower resolution than in the original paper to match the arxiv requirements

We present the detection of nuclear stellar discs in the low-luminosity elliptical galaxies NGC 4458 and NGC 4478, which are known to host a kinematically-decoupled core. Using archival HST imaging, and available absorption line-strength index data based on ground-based spectroscopy, we investigate the photometric parameters and the properties of the stellar populations of these central structures. Their scale length, h, and face-on central surface brightness, mu_0^c, fit on mu_0^c-h relation for galaxy discs. For NGC 4458 these parameters are typical for nuclear discs, while the same quantities for NGC 4478 lie between those of nuclear discs and the discs of discy ellipticals. We present Lick/IDS absorption line-strength measurements of Hbeta, Mgb, along the major and minor axes of the galaxies. We model these data with simple stellar populations that account for the alpha/Fe overabundance. The counter-rotating central disc of NGC 4458 is found to have similar properties to the decoupled cores of bright ellipticals. This galaxy has been found to be uniformly old despite being counter-rotating. In contrast, the cold central disc of NGC 4478 is younger, richer in metals and less overabundant than the main body of the galaxy. This points to a prolonged star formation history, typical of an undisturbed disc-like, gas-rich (possibly pre-enriched) structure., Comment: 11 pages, 8 figures, accepted for pubblication on MNRAS

At birth, the gastrointestinal tract is virtually sterile, but is rapidly colonized during the first days of life until a relatively stable state is reached. Several studies using both bacterial culture techniques and bio-molecular methods revealed that the amniotic cavity harbors microorganisms and, among them, uncultivated and uncharacterized taxa. The aim of the present PhD thesis was to evaluate the composition of some neglected populations inhabiting the infant gut microbiota until the second year of life. The PhD thesis is composed of three main chapters related to the presence of hydrogenotrophic populations, the occurrence of the Lachnospiraceae family in healthy subjects before the second year and the possible linkage between methanogens and diet in a piglet’s model. PCR-DGGE, the quantitative PCR (qPCR) and the Illumina deep sequencing have been the prevalent molecular techniques used in this work. Main conclusions obtained from these studies were mainly linked to the need of new primer sets that better describe the ecological complexity of the gut microbial community. This ambitious objective will be reached when it is possible to properly identify and quantify less represented bacterial populations within the infant gut ecosystem.

The purpose of this study was to assess the antimicrobial resistance profiles of several strains of Lactobacillus and Bifidobacterium spp. of probiotic interest. The International Organization for Standardization method was used to determine the minimal inhibitory concentrations of the antibiotics recommended by the European Food Safety Authority (EFSA). As a result, some of the tested microorganisms showed a visible growth up to the microbiological cutoff values indicated by EFSA guidelines in 2012. We were not able to categorize these strains as susceptible or resistant on the basis of antimicrobial resistance phenotypic testing as EFSA document does not explicitly deal with such a phenotypic condition where strains grow at antibiotic concentrations up to the established cutoff value. Although a few strains have been analyzed for this study, our findings highlight a potential challenge in accurately determining the antibiotic resistance in specific strains relevant for human and animal health.

The quality of chocolate is influenced by several parameters, one of which is bacterial diversity during fermentation and drying; a crucial factor for the generation of the optimal cocoa flavor precursors. Our understanding of the bacterial populations involved in chocolate fermentation can be improved by the use of high-throughput sequencing technologies (HTS), combined with PCR amplification of the 16S rRNA subunit. Here, we have conducted a high-throughput assessment of bacterial diversity in four processed samples of cocoa beans from different geographic origins. As part of this study, we also assessed whether different DNA extraction methods could affect the quality of our data. The dynamics of microbial populations were analyzed postharvest (fermentation and sun drying) and shipment, before entry to the industrial process. A total of 691,867 high quality sequences were obtained by Illumina MiSeq sequencing of the two bacterial 16S rRNA hypervariable regions, V3 and V4, following paired-read assembly of the raw reads. Manual curation of the 16S database allowed us to assign the correct taxonomic classifications, at species level, for 83.8% of those reads. This approach revealed a limited biodiversity and population dynamics for both the lactic acid bacteria (LAB) and acetic acid bacteria (AAB), both of which are key players during the acetification and lactic acid fermentation phases. Among the LAB, the most abundant species were Lactobacillus fermentum, Enterococcus casseliflavus, Weissella paramesenteroides, and Lactobacillus plantarum/paraplantarum. Among the AAB, Acetobacter syzygii, was most abundant, then Acetobacter senegalensis and Acetobacter pasteriuanus. Our results indicate that HTS approach has the ability to provide a comprehensive view of the cocoa bean microbiota at the species level.

Bariatric surgery has proven efficacy in the modulation of a number of gut peptides that can contribute to improvement of diabetes and its associated metabolic changes. In order to evaluate dietary intake, nutritional assessment and plasma levels of gastrointestinal peptides, we enrolled severely obese patients before and after bariatric surgery.We evaluated food intake, plasma levels of peptide YY (PYY), glucagon-like peptide-1/2 (GLP-1/2), ghrelin (GHR), orexin (ORE) and cholecystokinin (CCK), body composition and fecal microbiota in 28 severely obese patients and 28 healthy normal-weight controls. All parameters were evaluated at 0 time and 6 months after bariatric surgery.In obese patients we found a higher intake of nutrients, a decrease of free fat mass and an increase of BMI (body mass index), a significant decrease of GLP-1 and an increase of GLP-2, GHR and PYY with respect to controls, further increase in GLP-2, GHR and PYY, as well as increase over control values of GLP-1 after bariatric surgery. Obese individuals were found to harbor a community dominated by members of the Clostridial clusters XIVa and IV, whereas prominent bands after surgery were identified as Lactobacillus crispatus and Megasphaera elsdenii-related phylotype.The beneficial effects of bariatric surgery may at least in part be accounted for changes in circulating gastrointestinal (GI) peptides and fecal microbiota.

Probiotics have been linked to a reduction in the incidence of necrotizing enterocolitis and late-onset sepsis in preterm infants. Recently, probiotics have also proved to reduce time to achieve full enteral feeding (FEF). However, the relationship between FEF achievement and type of feeding in infants treated with probiotics has not been explored yet. The aim of this systematic review and meta-analysis was to evaluate the effect of probiotics in reducing time to achieve FEF in preterm infants, according to type of feeding (exclusive human milk (HM) vs. formula). Randomized-controlled trials involving preterm infants receiving probiotics, and reporting on time to reach FEF were included in the systematic review. Trials reporting on outcome according to type of feeding (exclusive HM vs. formula) were included in the meta-analysis. Fixed-effect or random-effects models were used as appropriate. Results were expressed as mean difference (MD) with 95% confidence interval (CI). Twenty-five studies were included in the systematic review. In the five studies recruiting exclusively HM-fed preterm infants, those treated with probiotics reached FEF approximately 3 days before controls (MD -3.15 days (95% CI -5.25/-1.05), p = 0.003). None of the two studies reporting on exclusively formula-fed infants showed any difference between infants receiving probiotics and controls in terms of FEF achievement. The limited number of included studies did not allow testing for other subgroup differences between HM and formula-fed infants. However, if confirmed in further studies, the 3-days reduction in time to achieve FEF in exclusively HM-fed preterm infants might have significant implications for their clinical management.

Approximately 20% of early-type galaxies host small nuclear stellar discs that are tens to a few hundred parsecs in size. Such discs are expected to be easily disrupted during major galactic encounters, hence their age serve to constrain their assembly history. We use VIMOS integral-field spectroscopic observations for the intermediate-mass E0 galaxy NGC4458 and age-date its nuclear disc via high-resolution fitting of various model spectra. We find that the nuclear disc is at least 6 Gyr old. A clue to gain narrow limits to the stellar age is our knowledge of the nuclear disc contribution to the central surface brightness. The presence of an old nuclear disk, or the absence of disruptive encounters since z~0.6, for a small galaxy such as NGC4458 which belongs to the Virgo cluster, may be consistent with a hierarchical picture for galaxy formation where the smallest galaxies assembles earlier and the crowded galactic environments reduce the incidence of galaxy mergers. On the other hand, NGC4458 displays little or no bulk rotation except for a central kpc-scale kinematically-decoupled core. Slow rotation and decoupled core are usually explained in terms of mergers. The presence and age of the nuclear disc constraint these mergers to have happened at high redshift., Comment: 10 pages, 8 figures. Accepted for publication on MNRAS

In the last 15 years, the term probiotic has achieved a consensus definition, and two Food and Agricultural Organization/World Health Organization documents have clarified and improved the regulatory profile of probiotics. In contrast, the definition of prebiotic proposed by the Food and Agricultural Organization remains under debate. In the last 5 years growing evidence has supported the suggestion that probiotics/psychobiotics can influence brain function and contribute to the amelioration or prevention of disease and mood disorders. From the regulatory point of view, these exciting results must be considered with prudence because of differences in the approaches and needs of peer reviewers of scientific journals versus examiners of regulatory administration. For conventional probiotics, enrollment of healthy versus unhealthy subjects into trials may define the development of a food or a pharmaceutical product.

The early-type spiral NGC 4698 is known to host a nuclear disc of gas and stars which is rotating perpendicularly with respect to the galaxy main disc. In addition, the bulge and main disc are characterised by a remarkable geometrical decoupling. Indeed they appear elongated orthogonally to each other. In this work the complex structure of the galaxy is investigated by a detailed photometric decomposition of optical and near-infrared images. The intrinsic shape of the bulge was constrained from its apparent ellipticity, its twist angle with respect to the major axis of the main disc, and the inclination of the main disc. The bulge is actually elongated perpendicular to the main disc and it is equally likely to be triaxial or axisymmetric. The central surface brightness, scalelength, inclination, and position angle of the nuclear disc were derived by assuming it is infinitesimally thin and exponential. Its size, orientation, and location do not depend on the observed passband. These findings support a scenario in which the nuclear disc is the end result of the acquisition of external gas by the pre-existing triaxial bulge on the principal plane perpendicular to its shortest axis and perpendicular to the galaxy main disc. The subsequent star formation either occurred homogeneously all over the extension of the nuclear disc or through an inside-out process that ended more than 5 Gyr ago.