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ビール酵母をクエン酸緩衝液添加硫安培地(窒素源を硫酸アンモンとする合成培地)で培養した場合に培地中に分泌されるUV吸収物質を活性炭吸着-アンモニア・エタノール混液溶出, Dowex 1 (ギ酸型)によるイオン交換樹脂クロマトグラフィーにより分画し,次のような結果を得た. (1) 静置培養時の分泌UV吸収物質のうち約25%はDowex 1に吸着されない非ヌクレオチド性のものであった. Dowex 1吸着区分はその70%あまりがCMP, AMP, GMP, UMPのモノヌクレオチドであり,その外にも未同定の少量の溶出区分が4つあった.ヌクレオチドのモル比はAMPを1とすると, CMP O.73, GMP O.93, UMP 1.40であった. (2) 振とう培養時の分泌UV吸収物質について同様の実験を行なった. Dowex 1非吸着区分は約22%,これは主にヌクレオシドであった.吸着区分の溶出パターンは分離がよかったため多数のピークが出現したが,基本的には静置培養の場合と同様モノヌクレオチドであった,モノヌクレオチドの組成も静置培養の場合と大差なかった. 5'-ヌクレオチドはGMPを除く各モノヌクレオチド区分に検出されたが,その割合は全体の20%足らずであった. 前報並びに以上の分析結果に基づいて分泌機作について考察し,分泌物は増殖中の酵母のRNAの分解物であろうと推定した.

Sixty patients have undergone surgical reconstruction of the injured thoraco-lumbar spine during the period 1968 to 1978 at the Spinal Unit of the National Murayama Hospital. The aim of treatment of any fracture, dislocation, or fracture-dislocation of the spine is to render it stable and painfree. This is best achieved by reduction and stabilisation of the involved segment of the spinal column. Where the posterior ligaments and intervertebral disc are ruptured healing of these structures with return to normal stability cannot be anticipated. In order to obtain permanent stability at the affected vertebral level we elect to perform anterior intervertebral fusion.

The dark-aerobically grown Rhodopseudomonas spheroides cells, which has no photosynthetic pigments, commenced to synthesize bacteriochlorophyll and carotenoids without detectable growth when the cells were transfered to dark semi-anaerobic incubation conditions. The level of δ-aminolevulinic acid synthetase increased over fourfold during the incubation. The induced synthesis of the pigments was almost completely inhibited by the addition of either acriflavin (2:8-diamino-10-methyl-acridine), chloramphenicol, mitomycin C or phleomycin to the incubation medium. The synthesis was also inhibited by actinomycin D and 8-azaguanine to considerable extents. The induced synthesis of δ-aminolevulinic acid synthetase, however, was scarcely affected by mitomycin C or phleomycin, whereas the synthesis was completely inhibited by chloramphenicol and acriflavin. The induced formation of catalase (H 2 O 2 : H 2 O 2 oxidoreductase, EC 1.11. 1.6) in light-anaerobically grown R. spheroides cells (brought about by incubating the cells under vigorous aeration) was not inhibited by mitomycin C but was inhibited by chloramphenicol and acriflavin. When mitomycin or acriflavin was added to the incubation mixture after the synthesis of pigments had commenced, the inhibition of the pigment synthesis became apparent only after an insusceptible period of about one hour, whereas the inhibition of δ-aminolevulinic acid synthetase began immediately after the addition of the inhibitors. The cells synthesizing photosynthetic pigments under dark semi-anaerobic conditions actively incorporated the radioactivity of [ 14 C]uracil into the DNA fraction as well as into the RNA fraction of the cells. The incorporation of radioactivity into both RNA and DNA was completely inhibited by acriflavin. Mitomycin inhibited the incorporation into DNA by 96%, whereas the inhibition by mitomycin of the incorporation into the RNA fraction was only 30%.