101. Gold nanorod-mediated photothermolysis induces apoptosis of macrophages via damage of mitochondria
- Author
-
Ji-Xin Cheng and Ling Tong
- Subjects
Pathology ,medicine.medical_specialty ,Materials science ,Necrosis ,Cell Membrane Permeability ,Confocal ,Biomedical Engineering ,Medicine (miscellaneous) ,Bioengineering ,Apoptosis ,Development ,Mitochondrion ,Mice ,Two-photon excitation microscopy ,apoptosis ,gold nanorods ,mitochondria damage ,necrosis ,photothermolysis ,primary activated macrophages ,two-photon luminescence imaging ,Cell Line, Tumor ,medicine ,Fluorescence microscope ,Animals ,General Materials Science ,Cysteine ,Biomedical Engineering and Bioengineering ,chemistry.chemical_classification ,Reactive oxygen species ,Nanotubes ,Cell Death ,Lasers ,Macrophages ,Macrophage Activation ,Photochemical Processes ,Mitochondria ,Nanoscience and Nanotechnology ,chemistry ,Biophysics ,Gold ,medicine.symptom ,Reactive Oxygen Species ,Oligopeptides ,Intracellular - Abstract
Aims: Induction of apoptosis or necrosis in activated macrophages by gold nanorod-mediated photothermolysis is demonstrated and the mechanisms underlying the processes are investigated. Materials & methods: Gold nanorods were functionalized with cysteine-octaarginine peptides (R8-NRs). Uptake of R8-NRs by activated macrophages was monitored by two-photon luminescence imaging. The laser irradiation conditions were controlled to induce apoptosis or necrosis to R8-NR-internalized macrophages. Mitochondrial damage and reactive oxygen species overproduction during photothermolysis was investigated by confocal fluorescence microscopy and transmission-electron microscopy. Results: Activated macrophages efficiently uptake R8-NRs both in vitro and in live animals. Laser irradiation of internalized nanorods with controlled power density induces apoptosis of macrophages via intracellular perturbation and subsequent injury of mitochondria. Conclusions: Gold nanorod-mediated photothermolysis provides one promising way to eliminate activated macrophages in autoimmune and inflammatory diseases.
- Published
- 2009