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1,253 results on '"Moore, Simon"'

101. Insights from Linking Police Domestic Violence and Health Data in South Wales: A Linked Routine Data Analysis Using Machine Learning

Author

Kennedy, Natasha, primary, Win, Tint Lwin, additional, Bandyopadhyay, Amrita, additional, Kennedy, Jonathan, additional, Rowe, Ben, additional, McNerney, Cynthia, additional, Evans, Julie, additional, Hughes, Karen, additional, Bellis, Mark A., additional, Jones, Angela, additional, Harrington, Karen, additional, Moore, Simon, additional, and Brophy, Sinead, additional

Published
2023
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104. Elucidation of the anaerobic biosynthesis of vitamin B₁₂ in Bacillus megaterium

Author

John Moore, Simon

Abstract

Vitamin B₁₂ (cobalamin) is one of Nature's most complex small molecules. It is a cobalt-containing modified tetrapyrrole that plays a number of key metabolic roles in many prokaryotic systems and is also essential to the biochemistry of higher animals. However, it is made by only certain bacteria, requiring around thirty enzymatic steps for its complete de novo construction. Surprisingly, the nutrient is synthesised by one of two related, though genetically distinct, pathways that represent aerobic or anaerobic routes. The anaerobic pathway has remained poorly characterised due to the instability of the pathway intermediates to oxygen and the low activity of enzymes, and is the focus of the research reported in this thesis. The Gram-positive aerobe Bacillus megaterium has previously been used for the commercial production of cobalamin and has a complete anaerobic pathway. Several genes, (termed chi for cobinamide biosynthesis) from the cobalamin biosynthetic pathway have been cloned and overexpressed individually within the host B. megaterium DSM319. One of the major bottlenecks in the anaerobic pathway is the ring contraction step, where only limited yields (

Published
2023
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106. POETS: An event-driven approach to Dissipative Particle Dynamics

Author

Brown, Andrew, Beaumont, Jonny, Thomas, David Barrie, Shillcock, Julian, Naylor, Matthew, Bragg, Graeme McLachlan, Vousden, Mark, Moore, Simon W., and Flemming, Shane

Abstract

HPC clusters have become ever more expensive, both in terms of capital cost and energy consumption - some estimates suggest that competitive installations at the end of the next decade will require their own power station. One way around this looming problem is to design bespoke computing engines, but while the performance benefits are good, the design costs are huge and cannot easily be amortized. POETS (Partially Ordered Event Triggered System) - the focus of this paper - seeks to exploit a middle way: the architecture is tuned to a specific algorithmic pattern, but within that constraint, is fully programmable. POETS software is quasi-imperative: the user defines a set of sequential event handlers, defines the topology of a (typically large) concurrent ensemble of these, and lets them interact. The 'solution' may be exfiltrated from the emergent behaviour of the ensemble. In this paper, we describe (briefly) the architecture, and an example computational chemistry application, dissipative particle dynamics (DPD). The DPD algorithm is traditionally implemented using parallel computational techniques, but we re-cast it as a concurrent compute problem which is then ideally suited to POETS. Our prototype system is realised on a cluster of 48 FPGAs providing 50K concurrent hardware threads, and we report performance speedups of over two orders of magnitude better than a single thread baseline comparator, and scaling behaviour that is almost constant. The results are validated against a "conventional" implementation.

Published
2022

108. Condition-based Maintenance of Overhead Travelling Cranes

Author

Lafarge, Damien, Colloca, Cristiana, Moore, Simon James, and Garcia Fernandez, A

Subjects
Engineering, Accelerators and Storage Rings
Abstract

The EN-HE group operates more than 350 overhead travelling cranes, requiring a regular maintenance de-fined by CERN Safety Rules (GSI-M-1). An analysis made in 2020 concluded that important savings on preven-tive maintenance could be done by changing the actual strategy based on a fixed periodicity to a strategy based on the usage of each crane. To be able to implement such change, the usage of each crane has to be known and remotely communicated on a daily basis. This prerequisite is being fulfilled thanks to an economic solution based on IoT devices connected to each crane and relying on new LoRa network in surface and the LTE (GSM) network in underground locations. This presentation focuses on the initial context including the financial stakes, the deployed solution based on IoT devices, the challenges, the results obtained and the possible perspectives for a wider usage at CERN.

Published
2022

113. The Frequency Injection Attack on Ring-Oscillator-Based True Random Number Generators

Author

Markettos, A. Theodore, Moore, Simon W., Hutchison, David, Series editor, Kanade, Takeo, Series editor, Kittler, Josef, Series editor, Kleinberg, Jon M., Series editor, Mattern, Friedemann, Series editor, Mitchell, John C., Series editor, Naor, Moni, Series editor, Nierstrasz, Oscar, Series editor, Pandu Rangan, C., Series editor, Steffen, Bernhard, Series editor, Sudan, Madhu, Series editor, Terzopoulos, Demetri, Series editor, Tygar, Doug, Series editor, Vardi, Moshe Y., Series editor, Weikum, Gerhard, Series editor, Clavier, Christophe, editor, and Gaj, Kris, editor

Published
2009
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117. Netrins and Their receptors

Author

Moore, Simon W., Tessier-Lavigne, Marc, Kennedy, Timothy E., Back, Nathan, editor, Cohen, Irun R., editor, Lajtha, Abel, editor, Lambris, John D., editor, Paoletti, Rodolfo, editor, and Bagnard, Dominique, editor

Published
2007
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129. A Vector Approach to Cryptography Implementation

Author

Fournier, Jacques J. A., Moore, Simon, Hutchison, David, Series editor, Kanade, Takeo, Series editor, Kittler, Josef, Series editor, Kleinberg, Jon M., Series editor, Mattern, Friedemann, Series editor, Mitchell, John C., Series editor, Naor, Moni, Series editor, Nierstrasz, Oscar, Series editor, Pandu Rangan, C., Series editor, Steffen, Bernhard, Series editor, Sudan, Madhu, Series editor, Terzopoulos, Demetri, Series editor, Tygar, Dough, Series editor, Vardi, Moshe Y., Series editor, Weikum, Gerhard, Series editor, Safavi-Naini, Reihaneh, editor, and Yung, Moti, editor

Published
2006
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130. My Compiler Really Understands Me: An Adaptive Programming Language Tutor

Author

Taylor, Kate, Moore, Simon, Hutchison, David, editor, Kanade, Takeo, editor, Kittler, Josef, editor, Kleinberg, Jon M., editor, Mattern, Friedemann, editor, Mitchell, John C., editor, Naor, Moni, editor, Nierstrasz, Oscar, editor, Pandu Rangan, C., editor, Steffen, Bernhard, editor, Sudan, Madhu, editor, Terzopoulos, Demetri, editor, Tygar, Dough, editor, Vardi, Moshe Y., editor, Weikum, Gerhard, editor, Wade, Vincent P., editor, Ashman, Helen, editor, and Smyth, Barry, editor

Published
2006
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131. Implementing Cryptography on TFT Technology for Secure Display Applications

Author

Oikonomakos, Petros, Fournier, Jacques, Moore, Simon, Hutchison, David, Series editor, Kanade, Takeo, Series editor, Kittler, Josef, Series editor, Kleinberg, Jon M., Series editor, Mattern, Friedemann, Series editor, Mitchell, John C., Series editor, Naor, Moni, Series editor, Nierstrasz, Oscar, Series editor, Pandu Rangan, C., Series editor, Steffen, Bernhard, Series editor, Sudan, Madhu, Series editor, Terzopoulos, Demetri, Series editor, Tygar, Dough, Series editor, Vardi, Moshe Y., Series editor, Weikum, Gerhard, Series editor, Domingo-Ferrer, Josep, editor, Posegga, Joachim, editor, and Schreckling, Daniel, editor

Published
2006
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132. Security Evaluation Against Electromagnetic Analysis at Design Time

Author

Li, Huiyun, Markettos, A. Theodore, Moore, Simon, Hutchison, David, editor, Kanade, Takeo, editor, Kittler, Josef, editor, Kleinberg, Jon M., editor, Mattern, Friedemann, editor, Mitchell, John C., editor, Naor, Moni, editor, Nierstrasz, Oscar, editor, Pandu Rangan, C., editor, Steffen, Bernhard, editor, Sudan, Madhu, editor, Terzopoulos, Demetri, editor, Tygar, Dough, editor, Vardi, Moshe Y., editor, Weikum, Gerhard, editor, Rao, Josyula R., editor, and Sunar, Berk, editor

Published
2005
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134. Non-deterministic event brokered computing

Author

Brown, Andrew, primary, Todman, Tim, additional, Luk, Wayne, additional, Thomas, David, additional, Vousden, Mark, additional, Bragg, Graeme, additional, Beaumont, Jonny, additional, Moore, Simon, additional, Yakovlev, Alex, additional, and Rafiev, Ashur, additional

Published
2022
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136. Security Evaluation of Asynchronous Circuits

Author

Fournier, Jacques J. A., Moore, Simon, Li, Huiyun, Mullins, Robert, Taylor, George, Goos, Gerhard, editor, Hartmanis, Juris, editor, van Leeuwen, Jan, editor, Walter, Colin D., editor, Koç, Çetin K., editor, and Paar, Christof, editor

Published
2003
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137. Observational and Methodological Issues in a Multi-Agency Community-Based Intervention to Reduce Excessive Drinking

Author

Moore, Simon and Shepherd, Jonathan

Abstract

This article describes the methodology and observational data from a project that sought to, first, investigate the prevalence of alcohol related harm and alcohol misuse in Cardiff's night-time economy and, second, deliver a targeted server training intervention to premises identified as at risk of misselling alcohol. The bulk of the work is centred on a novel breathalyser survey of drinkers conducted on one Saturday and one Friday each month for 12 months. Due to its novelty in a UK context and the potential value of objective alcohol misuse data, the survey provided important insights into appropriate methodologies for surveying night time economies as well as unique insights into the nature of alcohol misuse.

Published
2008
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141. A ubiquitous amino acid source for prokaryotic and eukaryotic cell-free transcription-translation systems

Author

Nagappa, Lakshmeesha N, Sato, Wakana, Alam, Farzana, Chengan, Kameshwari, Smales, Christopher Mark, von der Haar, Tobias, Polizzi, Karen M., Adamala, Katarzyna, Moore, Simon J., Nagappa, Lakshmeesha N, Sato, Wakana, Alam, Farzana, Chengan, Kameshwari, Smales, Christopher Mark, von der Haar, Tobias, Polizzi, Karen M., Adamala, Katarzyna, and Moore, Simon J.

Abstract

Cell-free gene expression (CFE) systems are an attractive tool for engineering within synthetic biology and for industrial production of high-value recombinant proteins. CFE reactions require a cell extract, energy system, amino acids, and DNA, to catalyse mRNA transcription and protein synthesis. To provide an amino acid source, CFE systems typically use a commercial standard, which is often proprietary. Herein we show that a range of common microbiology rich media (i.e., tryptone, peptone, yeast extract and casamino acids) unexpectedly provide an effective and low-cost amino acid source. We show that this approach is generalisable, by comparing batch variability and protein production in the following range of CFE systems: Escherichia coli (Rosetta™ 2 (DE3), BL21(DE3)), Streptomyces venezuelae and Pichia pastoris. In all CFE systems, we show equivalent or increased protein synthesis capacity upon replacement of the commercial amino acid source. In conclusion, we suggest rich microbiology media provides a new amino acid source for CFE systems with potential broad use in synthetic biology and industrial biotechnology applications.

Published
2022

142. A curcumin direct protein biosensor for cell-free prototyping

Author

Kennedy, Agata, Griffin, Guy, Freemont, Paul S, Polizzi, Karen M, Moore, Simon J., Kennedy, Agata, Griffin, Guy, Freemont, Paul S, Polizzi, Karen M, and Moore, Simon J.

Abstract

In synthetic biology, biosensors are routinely coupled with a gene expression system for detecting small molecules and physical signals. We reveal a fluorescent complex, based on the interaction of an coli double bond reductase ( CurA), as a detection unit with its substrate curcumin-we call this a direct protein (DiPro) biosensor. Using a cell-free synthetic biology approach, we use the CurA DiPro biosensor to fine tune 10 reaction parameters (cofactor, substrate, and enzyme levels) for cell-free curcumin biosynthesis, assisted through acoustic liquid handling robotics. Overall, we increase CurA-curcumin DiPro fluorescence within cell-free reactions by 78-fold. This finding adds to the growing family of protein-ligand complexes that are naturally fluorescent and potentially exploitable for a range of applications, including medical imaging to engineering high-value chemicals.

Published
2022

143. Streptomyces cell-free systems for natural product discovery and engineering

Author

Moore, Simon J., Lai, Hung-En, Li, Jian, Freemont, Paul S, Moore, Simon J., Lai, Hung-En, Li, Jian, and Freemont, Paul S

Abstract

Streptomyces bacteria are a major microbial source of natural products, which are encoded within so-called biosynthetic gene clusters (BGCs). This highlight discusses the emergence of native Streptomyces cell-free systems as a new tool to accelerate the study of the fundamental chemistry and biology of natural product biosynthesis from these bacteria. Cell-free systems provide a prototyping platform to study plug-and-play reactions in microscale reactions. So far, Streptomyces cell-free systems have been used to rapidly characterise gene expression regulation, access secondary metabolite biosynthetic enzymes, and catalyse cell-free transcription, translation, and biosynthesis of example natural products. With further progress, we anticipate the development of more complex systems to complement existing experimental tools for the discovery and engineering of natural product biosynthesis from Streptomyces and related high G + C (%) bacteria.

Published
2022

150. Weighting of risk factors for low birth weight: A linked routine data cohort study in Wales, UK.

Author

Bandyopadhyay, Amrita, primary, Jones, Hope, additional, Parker, Michael, additional, Marchant, Emily, additional, Evans, Julie, additional, Todd, Charlotte, additional, Rahman, Muhammad A, additional, Healy, James, additional, Win, Tint Lwin, additional, Rowe, Ben, additional, Moore, Simon, additional, Jones, Angela, additional, and Brophy, Sinead, additional

Published
2022
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