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103. A halophilic thermotolerant Bacillus isolated from a marine hot spring able to produce a new exopolysaccharide

Author

Maugeri T.L., Gugliandolo C., Caccamo D., Panico A., Lama L., Gambacorta A., and Nicolaus B.

Abstract

A halophilic, thermotolerant Bacillus strain (B3-15), isolated from water of a shallow, marine hot spring at Vulcano island (Eolian Islands, Italy), produced an exocellular polysaccharide at 165 mg l-1. It grew on kerosene as sole carbon source and was resistant to heavy metals. It is related to Bacillus licheniformis and its polysaccharide was a tetrasaccharide having manno-pyranosidic configuration.

Published
2002

104. Production and Characterization of exopolysaccharides excreted by thermophilic bacteria from shallow, marine hydrothermal vents of flegrean areas (Italy)

Author

Nicolaus B., Lama L., Panico A., Schiano Moriello V., Romano I., and Gambacorta A.

Subjects
mannano, fermentazione, ambiente idrotermale, struttura primaria, termofilo
Abstract

Thermophilic microorganisms, aerobic or facultatively anaerobic, endospore forming with growth optima temperatures in the range of 60-80°C, have been isolated from hot marine springs around Ischia and from hydrothermal wents in the gulf of Naples. Mucous colonies are been selected for the recovery of new strains producing exopolysaccharides. The selective media induced the production of the polysaccharides. Chemical and spectroscopic studies allowed as to establish the chemical structures of some polysaccharides.

Published
2002

127. Brevibacillus themoruber: a promising microbial cell factory for exopolysaccharide production.

Author

Yasar Yildiz, S., Anzelmo, G., Ozer, T., Radchenkova, N., Genc, S., Di Donato, P., Nicolaus, B., Toksoy Oner, E., and Kambourova, M.

Subjects
BACILLUS (Bacteria), MICROBIAL cells, MICROBIAL exopolysaccharides, THERMOPHILIC bacteria, BACTERIAL growth, HYDROGEN-ion concentration
Abstract

Aims This study aims to identify a high level exopolysaccharide ( EPS) producer thermophile that in turn could be used as a model organism to study the biological mechanisms and whole genome organization of EPS-producing thermophilic bacteria. Methods and Results Thermophilic isolates were screened, and then growth and EPS production of the best producer Brevibacillus thermoruber strain 423 were investigated under different carbon and nitrogen sources, temperature, pH and agitation rates. Rheological characterization revealed that the EPS behaved like a typical Newtonian fluid and viscosity of the EPS solution increased with increasing Ca2+ ion concentration. Chemical characterization by TLC, GC- MS, FT- IR and NMR suggested a heteropolymer structure with glucose as major monomer unit. High biocompatibility of pure EPS fractions suggested their potential use in biomedical applications. Conclusions This study reports on the comprehensive description of microbial production conditions as well as chemical, rheological and biological characterization of the EPS produced by B. thermoruber strain 423. The bioreactor cultures were found to reach two times higher yields and three times higher productivities when compared with literature. Significance and Impact of the Study Brevibacillus thermoruber strain 423 combined the advantages of its nonpathogenicity with the advantages of fast productivity and hence proved to be a very promising model organism and cell factory for microbial EPS production. [ABSTRACT FROM AUTHOR]

Published
2014
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131. Issues of laser plasma sources for soft x-ray projection lithography

Author

Shmaenok, L., primary, Bijkerk, F., additional, Louis, E., additional, van Honk, A., additional, van der Wiel, M.J., additional, Platonov, Yu., additional, Shevelko, A., additional, Mitrofanov, A., additional, Frowein, H., additional, Nicolaus, B., additional, Voβ, F., additional, and Désor, R., additional

Published
1994
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134. Production of exopolysaccharides from a thermophilic microorganism isolated from a marine hot spring in flegrean areas.

Author

Moriello, V. Schiano, Lama, L., Poli, A., Gugliandolo, C., Maugeri, T.L., Gambacorta, A., and Nicolaus, B.

Subjects
MICROBIAL exopolysaccharides, THERMOPHILIC microorganisms, HOT springs
Abstract

A thermophilic strain isolated from sea sand at Maronti, near Sant' Angelo (Ischia), is described. The organism grows well at an optimal temperature of 60 °C at pH 7.0. The thermophilic bacterium, named strain 4004, produces an exocellular polysaccharide (EPS) in yields of 90 mg/1. The EPS fraction was produced with all substrates tested, although a higher yield was obtained with sucrose or trehalose as sole carbon source. During growth, the EPS content was proportional to the biomass. Three fractions (EPS1, EPS2, EPS3) were obtained after purification. Quantitative monosaccharide analysis of the EPSs revealed the presence of mannose:glucose:galactose in a relative ratio of 0.5:1.0:0.3 in EPS1, mannose:glucose:galactose in a relative ratio of 1.0:0.3:trace in EPS2, and galactose:mannose:glucosamine:arabinose in a relative ratio of 1.0:0.8:0.4:0.2 in EPS3. The average molecular mass of EPS3 was determined to be 1×10[sup 6] Da. From comparison of the chemical shift values in ¹H and [sup 13]C spectra, we conclude that EPS3 presents a pentasaccharide repeating unit. [ABSTRACT FROM AUTHOR]

Published
2003
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141. Prevention of neointimal proliferation by immunosuppression in synthetic vascular grafts.

Author

Walpoth, B H, Pavlicek, M, Celik, B, Nicolaus, B, Schaffner, T, Althaus, U, Hess, O M, Carrel, T, and Morris, R E

Abstract

Immunosuppressive agents have been proposed to reduce neointimal hyperplasia in synthetic vascular grafts. Thus, the purpose of the present study was to evaluate the safety and efficacy of rapamycins (systemic vs. local vs. oral administration) and mycophenolate mofetil (MMF) to reduce intimal hyperplasia in infrarenal synthetic vascular grafts of the rat.

Published
2001
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142. Purification of the ADP-Ribosylating Enzyme from S. Solfataricusby Sdspolyacrylamide Gel Electrophoresis and Electroelution

Author

Faraone-Mennella, M. R., Discenza, A., Gambacorta, A., Nicolaus, B., and Farina, B.

Abstract

ABSTRACTThe ADPribosylating enzyme from the thermophilic archaeon S. solfataricuswas purified by a simple procedure which included preparative electrophoresis on a 0.1% SDS- polyacrylamide gel. The gel slice containing the enzymatic protein was cut out and the enzyme was solubilized by electroelution. The pure enzyme was obtained by chromatography of the electroeluted sample on a DNA-Sepharose column. The purified enzyme retained both its full activity and the structuring ability as a function of temperature increase.

Published
2000
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146. trehalose in archaebacteria

Author

Nicolaus B, Gambacorta A, Basso AL, Riccio R, Grant WD, DE ROSA, Mario, Nicolaus, B, Gambacorta, A, Basso, Al, Riccio, R, DE ROSA, Mario, and Grant, Wd

Published
1988

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