101. Overexpression of miRNA-9 enhances galectin-3 levels in oral cavity cancers.
- Author
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Wan Y, Zhang X, Tang KD, Blick T, Kenny L, Thompson EW, and Punyadeera C
- Subjects
- Apoptosis genetics, Blood Proteins metabolism, Cell Line, Tumor, Cell Movement genetics, Cell Proliferation genetics, Female, Galectin 3 genetics, Galectins metabolism, Gene Expression genetics, Gene Expression Profiling methods, Gene Expression Regulation, Neoplastic genetics, Head and Neck Neoplasms genetics, Humans, Male, MicroRNAs metabolism, Mouth, Mouth Neoplasms metabolism, Saliva chemistry, Transcriptome genetics, Blood Proteins genetics, Galectins genetics, MicroRNAs genetics, Mouth Neoplasms genetics
- Abstract
Oral cavity cancer (OCC) is the predominant subtype of head and neck cancer (HNC) and has up to 50% mortality. Genome-wide microRNA (miR) sequencing data indicates overexpression of miR-9-5p in HNC tumours, however, the biological role of miR-9-5p in OCC is complex; it can either act as a tumour suppressor or an oncomir, regulating many target genes at the post-transcriptional level. We have investigated the overexpression of miR-9-5p in three OCC cell lines. We have evaluated its expression levels and Galectin-3 as potential biomarkers in saliva samples collected from controls and OCC patients. We found that over expression of miR-9-5p in OCC cell lines resulted in a significant reduction in cell proliferation and migration, and an increase in apoptosis, which was paralleled by an increase in Galectin-3 secretion and export of Galectin-3 protein. Our data are consistent with miR-9-5p being a modulator of Galectin-3 via the AKT/γ-catenin pathway. In addition, the positive correlation between the levels of miR-9-5p expression and secreted Galectin-3 in saliva reflects a similar relationship in vivo, and supports the utility of their integrative evaluation in OCC. Our findings indicate that both miR-9-5p and Galectin-3 are critical biomolecules in the progression of OCC.
- Published
- 2021
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