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328 results on '"Raffa, Vittoria"'

105. A carbon nanotube based nanodevice for gene therapy

Author

Raffa, Vittoria, Ciofania, G, Vittorio, Orazio, Pizzorusso, T, Bardi, G, Costa, Mario, Kostarelos, K, Al Jamal, K, Zhiaei, A, Nitodas, S, Burghard, M, Santhosh, P, and Cuschieri, A.

Published
2009

109. Gene transfer mediated by carbon nanotubes

Author

Raffa, Vittoria, Vittorio, Orazio, Costa, Mario, Ziaei, A, Al Jamal, K. D., Pizzorusso, T, Bardi, G, Gherardini, L, Kostarelos, K, Nunes, A, Nitodas, S, Karachalios, T, Burghard, M, Santhosh, P, Amsharov, N, Riggio, Cristina, and Cuschieri, A.

Published
2009

132. Neuronal cells loaded with PEI-coated Fe3O4 nanoparticles for magnetically guided nerve regeneration

Author

Calatayud, M. Pilar, primary, Riggio, Cristina, additional, Raffa, Vittoria, additional, Sanz, Beatriz, additional, Torres, Teobaldo E., additional, Ibarra, M. Ricardo, additional, Hoskins, Clare, additional, Cuschieri, Alfred, additional, Wang, Lijun, additional, Pinkernelle, Josephine, additional, Keilhoff, Gerburg, additional, and Goya, Gerardo F., additional

Published
2013
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133. Multiwalled Carbon Nanotube Antennas Induce Effective Plasmid DNA Transfection of Bacterial Cells

Author

Raffa, Vittoria, primary, Vittorio, Orazio, additional, Costa, Mario, additional, Ziaei, Afshin, additional, Nitodas, Stephanos, additional, Riggio, Cristina, additional, Al-Jamal, Khuloud, additional, Gherardini, Lisa, additional, Bardi, Giuseppe, additional, Pizzorusso, Tommaso, additional, Karachalios, Theodoros, additional, and Cuschieri, Alfred, additional

Published
2012
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146. Sheets of Vertically Aligned BaTiO3 Nanotubes Reduce Cell Proliferation but Not Viability of NIH-3T3 Cells.

Author

Giannini, Marianna, Giannaccini, Martina, Sibillano, Teresa, Giannini, Cinzia, Liu, Dun, Wang, Zhigang, Baù, Andrea, Dente, Luciana, Cuschieri, Alfred, and Raffa, Vittoria

Subjects
CELL proliferation, BIOMATERIALS, TISSUE engineering, BARIUM titanate, NANOTUBES, BIOENGINEERING
Abstract

All biomaterials initiate a tissue response when implanted in living tissues. Ultimately this reaction causes fibrous encapsulation and hence isolation of the material, leading to failure of the intended therapeutic effect of the implant. There has been extensive bioengineering research aimed at overcoming or delaying the onset of encapsulation. Nanotechnology has the potential to address this problem by virtue of the ability of some nanomaterials to modulate interactions with cells, thereby inducing specific biological responses to implanted foreign materials. To this effect in the present study, we have characterised the growth of fibroblasts on nano-structured sheets constituted by BaTiO3, a material extensively used in biomedical applications. We found that sheets of vertically aligned BaTiO3 nanotubes inhibit cell cycle progression - without impairing cell viability - of NIH-3T3 fibroblast cells. We postulate that the 3D organization of the material surface acts by increasing the availability of adhesion sites, promoting cell attachment and inhibition of cell proliferation. This finding could be of relevance for biomedical applications designed to prevent or minimize fibrous encasement by uncontrolled proliferation of fibroblastic cells with loss of material-tissue interface underpinning long-term function of implants. [ABSTRACT FROM AUTHOR]

Published
2014
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147. Development of a flexible tag microlab

Author

Abad, Estefania, primary, Raffa, Vittoria Simona, additional, Mazzolai, Barbara, additional, Marco, Santiago, additional, Krenkow, Angelika, additional, and Becker, Thomas, additional

Published
2005
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149. Neuronal cells loaded with PEI-coated Fe3O4 nanoparticles for magnetically guided nerve regeneration.

Author

Calatayud, M. Pilar, Riggio, Cristina, Raffa, Vittoria, Sanz, Beatriz, Torres, Teobaldo E., Ibarra, M. Ricardo, Hoskins, Clare, Cuschieri, Alfred, Wang, Lijun, Pinkernelle, Josephine, Keilhoff, Gerburg, and Goya, Gerardo F.

Abstract

We report a one-step synthesis protocol for obtaining polymer-coated magnetic nanoparticles (MNPs) engineered for uploading neural cells. Polyethyleneimine-coated Fe3O4 nanoparticles (PEI-MNPs) with sizes of 25 ± 5 nm were prepared by oxidation of Fe(OH)2 by nitrate in basic aqueous media and adding PEI in situ during synthesis. The obtained PEI-MNP cores displayed a neat octahedral morphology and high crystallinity. The resulting nanoparticles were coated with a thin polymer layer of about 0.7–0.9 nm, and displayed a saturation magnetization value MS = 58 A m2 kg−1 at 250 K (64 A m2 kg−1 for T = 10 K). Cell uptake experiments on a neuroblastoma-derived SH-SY5Y cell line were undertaken over a wide time and MNP concentration range. The results showed a small decrease in cell viability for 24 h incubation (down to 70% viability for 100 μg ml−1), increasing the toxic effects with incubation time (30% cell survival at 100 μg ml−1 for 7 days of incubation). On the other hand, primary neuronal cells displayed higher sensitivity to PEI-MNPs, with a cell viability reduction of 44% of the control cells after 3 days of incubation with 50 μg ml−1. The amount of PEI-MNPs uploaded by SH-SY5Y cells was found to have a linear dependence on concentration. The intracellular distribution of the PEI-MNPs analyzed at the single-cell level by the dual-beam (FIB/SEM) technique revealed the coexistence of both fully incorporated PEI-MNPs and partially internalized PEI-MNP-clusters crossing the cell membrane. The resulting MNP-cluster distributions open the possibility of using these PEI-MNPs for magnetically driven axonal re-growth in neural cells. [ABSTRACT FROM AUTHOR]

Published
2013
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