101. Absence of a role for interleukin-13 in inflammatory bowel disease
- Author
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Federica Facciotti, Thomas T. MacDonald, Amir Ghanbari, Jens Geginat, Paolo Biancheri, Flavio Caprioli, Laura Rovedatti, Syed S. Hoque, Antonio Di Sabatino, Francesca Ammoscato, Gino Roberto Corazza, Renata Curciarello, I. Joe-Njoku, Paolo Giuffrida, Biancheri, P, Di Sabatino, A, Ammoscato, F, Facciotti, F, Caprioli, F, Curciarello, R, Hoque, S, Ghanbari, A, Joe-Njoku, I, Giuffrida, P, Rovedatti, L, Geginat, J, Corazza, G, and Macdonald, T
- Subjects
Adult ,Pathology ,medicine.medical_specialty ,Adolescent ,CD3 Complex ,Immunology ,Inflammation ,Biology ,Peripheral blood mononuclear cell ,Inflammatory bowel disease ,Transforming Growth Factor beta1 ,Interferon-gamma ,Young Adult ,Th2 Cells ,CD28 Antigens ,Crohn Disease ,medicine ,Humans ,Immunology and Allergy ,Intestinal Mucosa ,Crohn’s disease, Fibrosis, T helper cell type 2, Ulcerative colitis ,Aged ,Crohn's disease ,Lamina propria ,Interleukin-13 ,Mucous Membrane ,Macrophages ,Middle Aged ,Inflammatory Bowel Diseases ,medicine.disease ,Natural killer T cell ,Fibrosis ,Interleukin-13 Receptor alpha1 Subunit ,Ulcerative colitis ,Intestines ,medicine.anatomical_structure ,Interleukin 13 ,Interleukin-13 Receptor alpha2 Subunit ,Leukocytes, Mononuclear ,Natural Killer T-Cells ,Colitis, Ulcerative ,medicine.symptom - Abstract
IL-13 has been implicated in the pathogenesis of ulcerative colitis (UC), and may have a role in animal models of gut fibrosis. We studied the involvement of IL-13 in inflammation and fibrosis in UC and Crohn's disease (CD). Intestinal biopsies and anti-CD3/CD28- or anti-CD2/CD28-stimulated lamina propria mononuclear cells from UC and CD patients and control subjects were cultured, and IL-13, IL-4, IL-5, IL-17A and IFN-γ production was measured. Mucosal IL-13-producing cells were characterised by flow cytometry. Gut explants from strictured CD, non-strictured CD and healthy donors were cultured ex vivo, and secreted IL-13, IL-1β and collagen were measured. IL-13 production by mucosal explants and activated lamina propria mononuclear cells did not differ between CD, UC and control subjects, and was at least a log lower than IFN-γ and IL-17A. IL-13-producing cells, and in particular natural killer T cells, were uniformly low in all groups. IL-4 and IL-5 were undetectable in culture supernatants. Explants of CD strictures produced low amounts of IL-13, whereas IL-1β and collagen were elevated. We could not confirm that UC or strictured CD are associated with elevated IL-13 production. These data suggest that an anti-IL-13 Ab would not be an appropriate therapeutic strategy in inflammatory bowel disease.
- Published
- 2014