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540 results on '"Tsui, Stephen Kwok-Wing"'

103. Identification, functional annotation, and stability analysis of miRNA in Dermatophagoides pteronyssinus.

Author

Yang, Zhiyuan, Wan, Angel Tsz‐Yau, Liu, Xiao‐Yu, Xiong, Qing, Liu, Zhi‐Gang, and Tsui, Stephen Kwok‐Wing

Subjects
DERMATOPHAGOIDES pteronyssinus, MICRORNA, TRANSFER RNA, HOUSE dust mites, NON-coding RNA, TYPE I interferons
Abstract

To the Editor, House dust mite (HDM) is one of the major allergen sources worldwide which causes up to 85% of respiratory allergic diseases.[1] This species was regarded as the most prevalent source of indoor allergens in the United States and Europe. In an attempt to study the stability of mite miRNAs in the environment, we have performed a Taqman miRNA expression assay (Applied Biosystems) to test the quantity of mite-specific miRNA in household dust samples. This is the first systemic investigation of dust mite miRNAs and a comprehensive picture of miRNAs in DPT was shown. [Extracted from the article]

Published
2020
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106. Sequencing of Panax notoginseng genome reveals genes involved in disease resistance and ginsenoside biosynthesis

Author

Fan, Guangyi, primary, Fu, Yuanyuan, additional, Yang, Binrui, additional, Liu, Minghua, additional, Zhang, He, additional, Liang, Xinming, additional, Shi, Chengcheng, additional, Ma, Kailong, additional, Wang, Jiahao, additional, Liu, Weiqing, additional, Shao, Libin, additional, Huang, Chen, additional, Guo, Min, additional, Cai, Jing, additional, Wong, Andrew KC, additional, Li, Cheuk-Wing, additional, Zhuang, Dennis, additional, Chen, Ke-Ji, additional, Cong, Wei-Hong, additional, Sun, Xiao, additional, Chen, Wenbin, additional, Xu, Xun, additional, Tsui, Stephen Kwok-Wing, additional, Liu, Xin, additional, and Lee, Simon Ming-Yuen, additional

Published
2018
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109. High-quality assembly of Dermatophagoides pteronyssinus genome and transcriptome reveals a wide range of novel allergens

Author

Liu, Xiao-Yu, primary, Yang, Kevin Yi, additional, Wang, Ming-Qiang, additional, Kwok, Jamie Sui-Lam, additional, Zeng, Xi, additional, Yang, Zhiyuan, additional, Xiao, Xiao-Jun, additional, Lau, Carol Po-Ying, additional, Li, Ying, additional, Huang, Zhi-ming, additional, Ba, Jin-ge, additional, Yim, Aldrin Kay-Yuen, additional, Ouyang, Chun-Yan, additional, Ngai, Sai-Ming, additional, Chan, Ting-Fung, additional, Leung, Elaine Lai-Han, additional, Liu, Liang, additional, Liu, Zhi-Gang, additional, and Tsui, Stephen Kwok-Wing, additional

Published
2018
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119. Eriocalyxin B, a natural diterpenoid, inhibited VEGF-induced angiogenesis and diminished angiogenesis-dependent breast tumor growth by suppressing VEGFR-2 signaling

Author

Zhou, Xunian, primary, Yue, Grace Gar-Lee, additional, Liu, Minghua, additional, Zuo, Zhili, additional, Lee, Julia Kin-Ming, additional, Li, Mingyue, additional, Tsui, Stephen Kwok-Wing, additional, Fung, Kwok-Pui, additional, Sun, Handong, additional, Pu, Jianxin, additional, and Lau, Clara Bik-San, additional

Published
2016
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126. The human microbiota: a new direction in the investigation of thoracic diseases

Author

Hui, Anselm Wang-Hei, Lau, Hon-Wai, Chan, Tiffany Hoi-Tung, and Tsui, Stephen Kwok-Wing

Subjects
Review Article
Abstract

Advancements in next generation sequencing technology have provided means for the comprehensive profiling of the microbial community in the respiratory tract in both physiological and pathological conditions. Recent studies have analyzed the bacterial composition in the respiratory tract of chronic obstructive pulmonary disease (COPD), influenza and tuberculosis patients, and have identified novel targets that may potentially lead to secondary infections. Certain bacteria have also been found to regulate the lung immune system and have unexpected connections with respiratory diseases. Further studies in these areas are necessary to dissect the exact relationship between the dynamics of the microbiota and the health of the respiratory system.

Published
2013

127. Whole genome sequencing data of 1110 <italic>Mycobacterium tuberculosis</italic> isolates identifies insertions and deletions associated with drug resistance.

Author

Zeng, Xi, Kwok, Jamie Sui-Lam, Yang, Kevin Yi, Leung, Kenneth Siu-Sing, Shi, Mai, Yang, Zhiyuan, Yam, Wing-Cheong, and Tsui, Stephen Kwok-Wing

Subjects
DRUG resistance in bacteria, MYCOBACTERIUM tuberculosis, TUBERCULOSIS treatment, NUCLEOTIDE sequencing, GENETIC markers, THERAPEUTICS
Abstract

Background: Drug resistance in Mycobacterium tuberculosis (MTB) is one of the major challenges in tuberculosis (TB) treatment. However, known mutations cannot explain all of the cases of resistance and little research has focused on the relationship between insertions / deletions (indels) and drug resistance. Results: Here, we retrieved whole genome sequencing data of 743 drug-resistant MTB strains and 367 pan-susceptible strains from TB patients from the public domain to identify novel genomic markers of drug resistance. A total of 20 region markers containing genes and intergenic regions (IGRs) with significant statistical correlation with antibiotic resistance were revealed, four of which have been previously reported to be associated with drug resistance. In addition, 83 point markers containing frameshift (FS) mutations and IGR indels were also identified independently based on differences in their incidence rates between drug-sensitive and -resistant strains. Among the 83 point markers, eight indels were detected in known drug-associated genes or IGRs. Furthermore, the overlap between 20 region markers and 83 point markers further indicated their associations with drug resistance. The markers identified were involved in essential bacterial metabolic functions, including cell wall and transmembrane transporter functions. A strong correlation between FS mutations and mutations in DNA repair genes including I21V in alkA, R48G in mutT4 and P2R in nth was also found. Conclusions: This study identified a set of novel genetic markers with FS mutations and IGR indels associated with MTB drug resistance, which greatly broadens the pool of mutations related to MTB drug resistance. This insight may be important in identifying novel mechanisms of drug resistance in MTB. [ABSTRACT FROM AUTHOR]

Published
2018
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128. MiR‐25 Suppresses 3T3‐L1 Adipogenesis by Directly Targeting KLF4 and C/EBPα

Author

Liang, Wei‐Cheng, primary, Wang, Yan, additional, Liang, Pu‐Ping, additional, Pan, Xu‐Qing, additional, Fu, Wei‐Ming, additional, Yeung, Venus Sai‐Ying, additional, Lu, Ying‐Fei, additional, Wan, David Chi‐Cheong, additional, Tsui, Stephen Kwok‐Wing, additional, Tsang, Suk‐Ying, additional, Ma, Wen‐Bin, additional, Zhang, Jin‐Fang, additional, and Waye, Mary Miu‐Yee, additional

Published
2015
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131. Transcriptome analysis of leaves, roots and flowers of Panax notoginseng identifies genes involved in ginsenoside and alkaloid biosynthesis

Author

Liu, Ming-Hua, primary, Yang, Bin-Rui, additional, Cheung, Wai-Fung, additional, Yang, Kevin Yi, additional, Zhou, He-Feng, additional, Kwok, Jamie Sui-Lam, additional, Liu, Guo-Cheng, additional, Li, Xiao-Feng, additional, Zhong, Silin, additional, Lee, Simon Ming-Yuen, additional, and Tsui, Stephen Kwok-Wing, additional

Published
2015
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133. Familial Young-Onset Diabetes, Pre-Diabetes and Cardiovascular Disease Are Associated with Genetic Variants of DACH1 in Chinese

Author

Ma, Ronald Ching Wan, Lee, Heung Man, Lam, Vincent Kwok Lim, Tam, Claudia Ha Ting, Ho, Janice Siu Ka, Zhao, Hai-Lu, Guan, Jing, Kong, Alice Pik Shan, Lau, Eric, Zhang, Guozhi, Luk, Andrea, Wang, Ying, Tsui, Stephen Kwok Wing, Chan, Ting Fung, Hu, Cheng, Jia, Wei Ping, Park, Kyong Soo, Lee, Hong Kyu, Furuta, Hiroto, Nanjo, Kishio, Tai, E. Shyong, Ng, Daniel Peng-Keat, Tang, Nelson Leung Sang, Woo, Jean, Leung, Ping Chung, Xue, Hong, Wong, Jeffrey Tze Fei, Leung, Po Sing, Lau, Terrence C.K., Tong, Peter Chun Yip, Xu, Gang, Ng, Maggie Chor Yin, So, Wing Yee, Chan, Juliana Chung Ngor, Ma, Ronald Ching Wan, Lee, Heung Man, Lam, Vincent Kwok Lim, Tam, Claudia Ha Ting, Ho, Janice Siu Ka, Zhao, Hai-Lu, Guan, Jing, Kong, Alice Pik Shan, Lau, Eric, Zhang, Guozhi, Luk, Andrea, Wang, Ying, Tsui, Stephen Kwok Wing, Chan, Ting Fung, Hu, Cheng, Jia, Wei Ping, Park, Kyong Soo, Lee, Hong Kyu, Furuta, Hiroto, Nanjo, Kishio, Tai, E. Shyong, Ng, Daniel Peng-Keat, Tang, Nelson Leung Sang, Woo, Jean, Leung, Ping Chung, Xue, Hong, Wong, Jeffrey Tze Fei, Leung, Po Sing, Lau, Terrence C.K., Tong, Peter Chun Yip, Xu, Gang, Ng, Maggie Chor Yin, So, Wing Yee, and Chan, Juliana Chung Ngor

Abstract

In Asia, young-onset type 2 diabetes (YOD) is characterized by obesity and increased risk for cardiovascular disease (CVD). In a genome-wide association study (GWAS) of 99 Chinese obese subjects with familial YOD diagnosed before 40-year-old and 101 controls, the T allele of rs1408888 in intron 1 of DACH1(Dachshund homolog 1) was associated with an odds ratio (OR) of 2.49(95% confidence intervals: 1.57-3.96, P = 8.4610(-5)). Amongst these subjects, we found reduced expression of DACH1 in peripheral blood mononuclear cells (PBMC) from 63 cases compared to 65 controls (P = 0.02). In a random cohort of 1468 cases and 1485 controls, amongst top 19 SNPs from GWAS, rs1408888 was associated with type 2 diabetes with a global P value of 0.0176 and confirmation in a multiethnic Asian case-control cohort (7370/7802) with an OR of 1.07(1.02-1.12, P-meta = 0.012). In 599 Chinese non-diabetic subjects, rs1408888 was linearly associated with systolic blood pressure and insulin resistance. In a case-control cohort (n = 953/953), rs1408888 was associated with an OR of 1.54(1.07-2.22, P = 0.019) for CVD in type 2 diabetes. In an autopsy series of 173 non-diabetic cases, TT genotype of rs1408888 was associated with an OR of 3.31(1.19-9.19, P = 0.0214) and 3.27(1.25-11.07, P = 0.0184) for coronary heart disease (CHD) and coronary arteriosclerosis. Bioinformatics analysis revealed that rs1408888 lies within regulatory elements of DACH1 implicated in islet development and insulin secretion. The T allele of rs1408888 of DACH1 was associated with YOD, prediabetes and CVD in Chinese.

Published
2014

134. Mutations Enabling Displacement of Tryptophan by 4-Fluorotryptophan as a Canonical Amino Acid of the Genetic Code

Author

Yu, Allen Chi Shing, Yim, Aldrin Kay Yuen, Mat, Wai Kin, Tong, Amy Hin Yan, Lok, Si, Xue, Hong, Tsui, Stephen Kwok Wing, Wong, Jeffrey Tze Fei, Chan, Ting Fung, Yu, Allen Chi Shing, Yim, Aldrin Kay Yuen, Mat, Wai Kin, Tong, Amy Hin Yan, Lok, Si, Xue, Hong, Tsui, Stephen Kwok Wing, Wong, Jeffrey Tze Fei, and Chan, Ting Fung

Abstract

The 20 canonical amino acids of the genetic code have been invariant over 3 billion years of biological evolution. Although various aminoacyl-tRNA synthetases can charge their cognate tRNAs with amino acid analogs, there has been no known displacement of any canonical amino acid from the code. Experimental departure from this universal protein alphabet comprising the canonical amino acids was first achieved in the mutants of the Bacillus subtilis QB928 strain, which after serial selection and mutagenesis led to the HR23 strain that could use 4-fluorotryptophan (4FTrp) but not canonical tryptophan (Trp) for propagation. To gain insight into this displacement of Trp from the genetic code by 4FTrp, genome sequencing was performed on LC33 (a precursor strain of HR23), HR23, and TR7 (a revertant of HR23 that regained the capacity to propagate on Trp). Compared with QB928, the negative regulator mtrB of Trp transport was found to be knocked out in LC33, HR23, and TR7, and sigma factor sigB was mutated in HR23 and TR7. Moreover, rpoBC encoding RNA polymerase subunits were mutated in three independent isolates of TR7 relative to HR23. Increased expression of sigB was also observed in HR23 and in TR7 growing under 4FTrp. These findings indicated that stabilization of the genetic code can be provided by just a small number of analog-sensitive proteins, forming an oligogenic barrier that safeguards the canonical amino acids throughout biological evolution.

Published
2014

135. Whole-genome bisulfite sequencing of multiple individuals reveals complementary roles of promoter and gene body methylation in transcriptional regulation

Author

Lou, Shaoke, Lee, Heung-Man, Qin, Hao, Li, Jing-Woei, Gao, Zhibo, Liu, Xin, Chan, Landon L., Lam, Vincent K. L., So, Wing-Yee, Wang, Ying, Lok, Si, Wang, Jun, Ma, Ronald C. W., Tsui, Stephen Kwok-Wing, Chan, Juliana C. N., Chan, Ting-Fung, Yip, Kevin Y., Lou, Shaoke, Lee, Heung-Man, Qin, Hao, Li, Jing-Woei, Gao, Zhibo, Liu, Xin, Chan, Landon L., Lam, Vincent K. L., So, Wing-Yee, Wang, Ying, Lok, Si, Wang, Jun, Ma, Ronald C. W., Tsui, Stephen Kwok-Wing, Chan, Juliana C. N., Chan, Ting-Fung, and Yip, Kevin Y.

Published
2014

139. Whole-genome bisulfite sequencing of multiple individuals reveals complementary roles of promoter and gene body methylation in transcriptional regulation

Author

Lou, Shaoke, primary, Lee, Heung-Man, additional, Qin, Hao, additional, Li, Jing-Woei, additional, Gao, Zhibo, additional, Liu, Xin, additional, Chan, Landon L, additional, KL Lam, Vincent, additional, So, Wing-Yee, additional, Wang, Ying, additional, Lok, Si, additional, Wang, Jun, additional, Ma, Ronald CW, additional, Tsui, Stephen Kwok-Wing, additional, Chan, Juliana CN, additional, Chan, Ting-Fung, additional, and Yip, Kevin Y, additional

Published
2014
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142. Familial Young-Onset Diabetes, Pre-Diabetes and Cardiovascular Disease Are Associated with Genetic Variants of DACH1 in Chinese

Author

Ma, Ronald Ching Wan, primary, Lee, Heung Man, additional, Lam, Vincent Kwok Lim, additional, Tam, Claudia Ha Ting, additional, Ho, Janice Siu Ka, additional, Zhao, Hai-Lu, additional, Guan, Jing, additional, Kong, Alice Pik Shan, additional, Lau, Eric, additional, Zhang, Guozhi, additional, Luk, Andrea, additional, Wang, Ying, additional, Tsui, Stephen Kwok Wing, additional, Chan, Ting Fung, additional, Hu, Cheng, additional, Jia, Wei Ping, additional, Park, Kyong Soo, additional, Lee, Hong Kyu, additional, Furuta, Hiroto, additional, Nanjo, Kishio, additional, Tai, E. Shyong, additional, Ng, Daniel Peng-Keat, additional, Tang, Nelson Leung Sang, additional, Woo, Jean, additional, Leung, Ping Chung, additional, Xue, Hong, additional, Wong, Jeffrey, additional, Leung, Po Sing, additional, Lau, Terrence C. K., additional, Tong, Peter Chun Yip, additional, Xu, Gang, additional, Ng, Maggie Chor Yin, additional, So, Wing Yee, additional, and Chan, Juliana Chung Ngor, additional

Published
2014
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144. LIM-only protein FHL3 interacts with CDC25B2 phosphatase

Author

BALDIN, Véronique, Mils, Valérie, Yuen Lee, Simon Ming, Joly, Willy, Hang, Eric Wong Chi, Baldin, V.éronique, Waye, Mary Miu Yee, Ducommun, Bernard, Tsui, Stephen Kwok Wing, Centre de recherches de biochimie macromoléculaire (CRBM), Centre National de la Recherche Scientifique (CNRS)-IFR122-Université Montpellier 2 - Sciences et Techniques (UM2)-Université Montpellier 1 (UM1), Laboratoire de Biologie Cellulaire et Moléculaire du Contrôle de la Prolifération (LBCMCP), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre de Biologie Intégrative (CBI), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut des Neurosciences de Montpellier - Déficits sensoriels et moteurs (INM), and Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)

Subjects
Gene isoform, Recombinant Fusion Proteins, Phosphatase, Cell Cycle Proteins, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], Biology, Cell Line, 03 medical and health sciences, Two-Hybrid System Techniques, Fluorescence Resonance Energy Transfer, Animals, Humans, Protein Isoforms, cdc25 Phosphatases, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, LIM domain, Cell Nucleus, Homeodomain Proteins, Focal Adhesions, 0303 health sciences, Cell Cycle, 030302 biochemistry & molecular biology, Intracellular Signaling Peptides and Proteins, FHL3, Cell Biology, LIM Domain Proteins, Cell cycle, 3. Good health, Cell biology, Cytoplasm, LHX3, C2C12, Protein Binding
Abstract

LIM domain proteins are important regulators of the growth, determination, and differentiation of cells. In this report, FHL3 (human four-and-a-half LIM-only protein 3) is shown to interact with human phosphatase CDC25B, a cell cycle regulator involved in the control of G2/M. We found that this interaction was specific to the CDC25B2 isoform. Deletion and point mutation studies indicated that the second LIM domain of FHL3 was essential for this interaction. FRET experiments in C2C12 cells showed that, although both proteins were colocated in the cytoplasm and the nucleus, they interacted only in the nucleus. Finally, we showed that FHL3 binding impaired neither CDC25B2 phosphatase activity nor its localization. Further work is now needed to elucidate the consequences of this interaction on myoblast fate decision and cycle control.

Published
2003

145. Inferring a protein interaction map of Mycobacterium tuberculosis based on sequences and interologs

Author

Liu, Zhiping, Wang, Jiguang, Qiu, Yuqing, Leung, Ross Ka Kit, Zhang, Xiangsun, Tsui, Stephen Kwok-Wing, Chen, Luonan, Liu, Zhiping, Wang, Jiguang, Qiu, Yuqing, Leung, Ross Ka Kit, Zhang, Xiangsun, Tsui, Stephen Kwok-Wing, and Chen, Luonan

Abstract

Background: Mycobacterium tuberculosis is an infectious bacterium posing serious threats to human health. Due to the difficulty in performing molecular biology experiments to detect protein interactions, reconstruction of a protein interaction map of M. tuberculosis by computational methods will provide crucial information to understand the biological processes in the pathogenic microorganism, as well as provide the framework upon which new therapeutic approaches can be developed.Results: In this paper, we constructed an integrated M. tuberculosis protein interaction network by machine learning and ortholog-based methods. Firstly, we built a support vector machine (SVM) method to infer the protein interactions of M. tuberculosis H37Rv by gene sequence information. We tested our predictors in Escherichia coli and mapped the genetic codon features underlying its protein interactions to M. tuberculosis. Moreover, the documented interactions of 14 other species were mapped to the interactome of M. tuberculosis by the interolog method. The ensemble protein interactions were validated by various functional relationships, i.e., gene coexpression, evolutionary relationship and functional similarity, extracted from heterogeneous data sources. The accuracy and validation demonstrate the effectiveness and efficiency of our framework.Conclusions: A protein interaction map of M. tuberculosis is inferred from genetic codons and interologs. The prediction accuracy and numerically experimental validation demonstrate the effectiveness and efficiency of our method. Furthermore, our methods can be straightforwardly extended to infer the protein interactions of other bacterial species. © 2012 Liu et al.; licensee BioMed Central Ltd.

Published
2012

149. Insertion/Deletion Polymorphisms in the ΔNp63 Promoter Are a Risk Factor for Bladder Exstrophy Epispadias Complex

Author

Wilkins, Simon, primary, Zhang, Ke Wei, additional, Mahfuz, Istiak, additional, Quantin, Renaud, additional, D'Cruz, Nancy, additional, Hutson, John, additional, Ee, Michael, additional, Bagli, Darius, additional, Aitken, Karen, additional, Fong, Fion Nga-Yin, additional, Ng, Patrick Kwok-Shing, additional, Tsui, Stephen Kwok-Wing, additional, Fung, Wendy Yin-Wan, additional, Banu, Tahmina, additional, Thakre, Atul, additional, Johar, Kaid, additional, Jaureguizar, Enrique, additional, Li, Long, additional, and Cheng, Wei, additional

Published
2012
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