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Our previous data confirmed that 1,8-Cineole had an antihypertensive effect in animal models. However, it is unclear whether antihypertension is dependent on the protective effect of 1,8-Cineole on endothelial function and structure. At present, the purpose was to investigate the protective effects of 1,8-Cineole on vascular endothelial tissue in hypertensive rats and human umbilical vein endothelial cells (HUVECs). Our results showed that 1,8-Cineole significantly reduced the blood pressure and improved the vascular endothelial lesion, attenuated vascular oxidative stress and inflammation induced by Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME) in rats. Pretreatment with 1,8-Cineole was able to inhibit the increase in malondialdehyde (MDA) and reactive oxygen species (ROS) induced by L-NAME, and increased the release and expression of superoxide dismutase (SOD) and nitric oxide (NO). In addition, 1,8-Cineole also reversed the increase of autophagy-associated protein LC3Ⅱ/LC3Ⅰ and the decrease of P62 in vivo and in vitro respectively. There was a synergistic effect between PI3K agonists and drugs, while PI3K inhibitors blocked the efficacy of 1,8-Cineole. The addition of autophagy inhibitor chloroquine increases the expression of eNOS. Taken together, our results indicate that 1,8-Cineole has potential beneficial promising antihypertension depending on the integrity of vascular endothelial structure and function induced by L-NAME, and the mechanism involves ameliorating autophagy by regulating of PI3K/mTOR. [ABSTRACT FROM AUTHOR]

[Display omitted] • Plasma working with grounded metal mesh is difficult to realize effective polymer etching. • The etched surface without metal mesh has higher etching rate and rougher surface. • Higher surface oxidation was obtained with the participation of charged particles. • Synergetic etching process of charged particles and reactive species was discussed. Atmospheric pressure plasma jet has shown great potential for polymer film treatment, but the etching process of polymer surface by the plasma jet is still not developed. In this paper, the effect of a grounded metal mesh addition on the etching process of polymer surface through the plasma jet was investigated. Comparative study of polymer film treated by the plasma jet with and without a grounded stainless steel mesh (SSM) is systematically investigated by the analysis of etching rate, surface morphologies and chemical compositions. Compared with the plasma etching process with SSM, the etching results without the addition of SSM have much higher etching rate, rougher etched surface, and higher surface oxidation. Finally, the etching process occurring at the plasma/polymer interface was also discussed. Since the addition of SSM could filter out the charged particles in plasma jet, the role of charged particles in the etching process was discussed and the synergetic etching process was proposed based on the structural dependence and chemical analysis of the observed results. [ABSTRACT FROM AUTHOR]

Vascular endothelial cell senescence is associated with cardiovascular complications in diabetes. Essential oil from Fructus Alpiniae zerumbet (Pers.) B.L.Burtt & R.M.Sm. (EOFAZ) has potentially beneficial and promising diabetes-related vascular endothelial cell senescence-mitigating effects; however, the underlying molecular mechanisms remain unclear. To investigate the molecular effects of EOFAZ on vascular endothelial cell senescence in diabetes. A diabetes mouse model was developed using a high-fat and high-glucose diet (HFD) combined with intraperitoneal injection of low-dose streptozotocin (STZ, 30 mg/kg) and oral treatment with EOFAZ. 4D label-free quantitative proteomics, network pharmacology, and molecular docking techniques were employed to explore the molecular mechanisms via which EOFAZ alleviates diabetes-related vascular endothelial cell senescence. A human aortic endothelial cells (HAECs) senescence model was developed using high palmitic acid and high glucose (PA/HG) concentrations in vitro. Western blotting, immunofluorescence, SA-β-galactosidase staining, cell cycle, reactive oxygen species (ROS), cell migration, and enzyme linked immunosorbent assays were performed to determine the protective role of EOFAZ against vascular endothelial cell senescence in diabetes. Moreover, the PPAR-γ agonist rosiglitazone, inhibitor GW9662, and siRNA were used to verify the underlying mechanism by which EOFAZ combats vascular endothelial cell senescence in diabetes. EOFAZ treatment ameliorated abnormal lipid metabolism, vascular histopathological damage, and vascular endothelial aging in diabetic mice. Proteomics and network pharmacology analysis revealed that the differentially expressed proteins (DEPs) and drug-disease targets were associated with the peroxisome proliferator-activated receptor gamma (PPAR-γ) signalling pathway, a key player in vascular endothelial cell senescence. Molecular docking indicated that the small-molecule compounds in EOFAZ had a high affinity for the PPAR-γ protein. Western blotting and immunofluorescence analyses confirmed the significance of DEPs and the involvement of the PPAR-γ signalling pathway. In vitro , EOFAZ and rosiglitazone treatment reversed the effects of PA/HG on the number of senescent endothelial cells, expression of senescence-related proteins, the proportion of cells in the G0/G1 phase, ROS levels, cell migration rate, and expression of pro-inflammatory factors. The protective effects of EOFAZ against vascular endothelial cell senescence in diabetes were aborted following treatment with GW9662 or PPAR-γ siRNA. EOFAZ ameliorates vascular endothelial cell senescence in diabetes by activating PPAR-γ signalling. The results of the present study highlight the potential beneficial and promising therapeutic effects of EOFAZ and provide a basis for its clinical application in diabetes-related vascular endothelial cell senescence. The molecular effects of EOFAZ on vascular endothelial cell senescence in diabetes using 4D label-free quantitative proteomics and network pharmacology. [Display omitted] • EOFAZ mitigates lipid dysfunction, vascular damage, and vascular aging in diabetes. • Targeting diabetic vascular endothelial senescence involved PPAR-γ signalling. • EOFAZ ameliorated vascular endothelial senescence in diabetes by regulating PPAR-γ signalling. [ABSTRACT FROM AUTHOR]

1,8-Cineole, the main component of volatile oil in aromatic plants, has diverse pharmacological properties, including antioxidant, anti-inflammatory, and anti-cancer properties. Diabetic retinopathy (DR) is a common microvascular complication of diabetes mellitus (DM). Here, we investigated the protective effect of 1,8-cineole on DR and found that 1,8-cineole treatment could alter the expression of several genes in both high glucose (HG)-induced ARPE-19 cells and retinal tissues of DM mice, as well as inhibit ferroptosis. Subsequent investigations into the molecular mechanisms underlying this inhibition revealed that expression of thioredoxin-interacting protein (TXNIP) was significantly upregulated while that of peroxisome proliferator-activated receptor γ (PPAR-γ) was significantly downregulated in HG-induced ARPE-19 cells, and treatment with 1,8-cineole could effectively reverse these changes. Treatment with a PPAR-γ pharmacological agonist (rosiglitazone), alone or combined with 1,8-cineole, significantly inhibited the transcription of TXNIP and ferroptosis in HG-induced ARPE-19 cells. Conversely, pretreatment with GW9662, a PPAR-γ inhibitor, upregulated the transcription and expression of TXNIP in HG-induced ARPE-19 cells; 1,8-cineole failed to reverse this upregulated expression. To explore these relationships, we constructed a PPAR-γ adenovirus shRNA to elucidate the effect of 1,8-cineole on the negative regulation of TXNIP by PPAR-γ. Taken together, the present findings indicate that HG-induced ferroptosis in retinal tissue plays an essential role in the pathogenesis of DR, which can be ameliorated by 1,8-cineole. [Display omitted] • 1,8-Cineole inhibits ferroptosis and prevents disruption of the outer blood retinal barrier in diabetic mice. • 1,8-Cineole inhibits high glucose (HG)-induced tight junction loss in vitro. • 1,8-Cineole suppresses HG-induced ferroptosis in retinal pigment epithelial cells. • 1,8-Cineole ameliorates diabetic retinopathy by activating PPAR-γ/TXNIP pathways. [ABSTRACT FROM AUTHOR]

• The tar yield is controlled by the type and content of hydrogen. • Weak bonds exhibit the most significant impact on the tar yield. • The molecular structures are controlled by the coal rank. • The molecular structures are influenced by the depositional environment. Coal with a tar yield ≥ 7 %, which is measured in the Gray-King assay, is called tar-rich coal in China. However, the comprehensive impact of coal ranks and depositional environments on tar yield is still unclear. Here, the coal petrology, molecular structure, and depositional environment of the samples were thoroughly investigated to clarify the influence of the coal rank and depositional environment on the tar-rich coal. The difference in the tar yield is attributed to the molecular structure. Concerning Fourier transform infrared (FTIR) spectroscopy, the high tar production resulted from the existence of relatively long aliphatic side chains and high aliphatic hydrogen content. For the 13C nuclear magnetic resonance analysis, the structure of the aliphatic carbon also played a crucial role in tar formation. Among these, the various unstable structural parameters (f al H / f a s , (f a s + f a p)/ f a ' , f a s / f a ' , f al H / f al *) and weak bonds could improve the tar yield. Furthermore, the molecular structures were also controlled by the coal rank. More specifically, the CH 2 /CH 3 in FTIR and the f al H , f al H / f a * , f a s / f a ' in 13C NMR had similar patterns with coal rank, which was close to the pattern between the coal rank and the tar yield. This effect also indicated that the tar yield was mainly influenced by bridge bonds, aliphatic side chains, and branched aromatic carbon. Between similar coal ranks, the molecular structure differences originated from the depositional environment. For the coal facies, the tissue preservation index (TPI) and vegetation index (VI) were negatively correlated with CH 2 /CH 3 , Hal/H, and "A factor". In contrast, they were positively correlated with the oxidation degree (Io 2). The gelification index (GI) was also positively correlated with CH 2 /CH 3 and Hal/H. Consequently, the coal-forming environment with a relatively high degree of decomposition, low woody tissue content, and high gelification can form key molecular structures easier, which is beneficial for the tar yield. [ABSTRACT FROM AUTHOR]

[Display omitted] • The nanocomposites could adhere bacteria via intetact with bacterial membrane. • AuNRs@PBA r PEGMA could protect macrophages from P. aeruginosa infection. • The tissue penetration depth of AuNRs@PBA r PEGMA + NIR was up to 12 nm in pork muscle. • AuNRs@PBA r PEGMA could effectively treat P. aeruginosa lung infection. Lung infection associated with drug-resistant P. aeruginosa and their wide spread have become a serious problem to public health. Effective antibacterial strategy without antibiotics must be developed for treatment of these infections. To combat pneumonia infected by multidrug-resistant P. aeruginosa (MRPA), a novel antibacterial nanocomoposite of AuNRs@PBA r PEGMA was prepared. AuNRs@PBA r PEGMA could bind with P. aeruginosa through the interaction betweeen PBA r PEGMA and bacteria proved by alizarin red assay. The nanocomposite could relieve inflammtory response in MRPA-infected macrophages by downregulating MAPK and NF κ B signal-transduction pathways. AuNRs@PBA r PEGMA showed well photo-thermal conversion capacity leading to the temperature increasing from 20 to 80 °C under near-infrared (NIR) irradiation for 5 min. The photo-thermal capacity endowed the nanocomposites efficient antibacterial activity against MRPA. Simultaneously, AuNRs@PBA r PEGMA with NIR irradiation could kill biofilm bacteria while traditional antibiotics were non-effetive. AuNRs@PBA r PEGMA with NIR irradiation could treat MRPA-related lung infection. The nanocomposite with NIR reduced the inflammatory cytokines of TNF-α, IL-1β, and IL-6 by downregulating the MAPK signal pathway in bronchoalveolar lavage fluids. The number of P. aeruginosa and expression of NF-κB p65 in lung also decreased. Above all, our study provides a non-antibiotic therapeutic strategy in clinical application and demonstrated that AuNRs@PBA r PEGMA with NIR irradiation showed a potential treatment for drug-resistant P. aeruginosa infection in Lung. [ABSTRACT FROM AUTHOR]

Underground coal thermal treatment is a clean method of coal utilisation. Pyrolytic semi-coke provides a good storage vehicle for CO 2 geological storage, but there are few studies on the CO 2 adsorption capacity of pyrolytic semi-coke. To investigate the CO 2 storage potential of thermal treated coals, scanning electron microscopy, low temperature nitrogen adsorption, low-field nuclear magnetic resonance and thermogravimetric analysis were used in this paper to analyze the differences and the influence mechanisms of different thermal treated coals for CO 2 adsorption. The results showed the CO 2 maximum adsorption amount of different thermal treated coals decreased and then increased as the thermal treatment temperature increased, reaching a minimum at 773.15 K. Although the maximum adsorption amount of 773.15 K thermal treated raw coal was disadvantageous, the gas pressure <4.49 MPa showed superior adsorption advantage over the 303.15 K thermal treated coal. However, the KCl-impregnated coal showed an overall low adsorption capacity attributed to crystal blocking effects and catalysis. Although the adsorption pores of the high temperature thermal treated coals were substantially reduced, their ability to adsorb CO 2 was significantly higher than that of the low temperature thermal treated coals. Furthermore, the number of adsorption pores was closely related to CO 2 adsorption. • The pyrolytic semi-coke based on UCTT is a good vehicle for CO 2 storage. • The swelling effect of the coal at 573.15 K reduced the adsorption properties. • High temperature treated coal have fewer adsorption pores but high adsorptivity. • 773.15 K thermal treated coal has good CO 2 adsorptivity and seepage capacity. [ABSTRACT FROM AUTHOR]

Tar yield, as a basic evaluation index of tar-rich coal, is unclear to be affected by the depositional environment. In this work, the vertical variations in tar yield, macerals, and major/trace elements were investigated for highly volatile bituminous coal in the Ordos Basin, China, to analyse the effect of the depositional environment on the tar-rich coal distribution. The results show that the tar yield more than 7% (tar-rich coal) accounts for 70% in this formation, which formed in a depositional environment with a stable supply of terrestrial sediments, a warm and humid climate, and weakly reducing to reducing conditions. For the coal facies, all samples were distributed into two groups of coal facies, with the first group containing limnic, dry forest swamp, wet forest swamp and another group contains bog, forest bog. Moreover, limnic and bog with less woody tissue and a higher water table contribute to the higher tar yield of coal. Correspondingly, the vitrinite content and the gelation index (GI) were positively correlated with tar yield, and the tissue preservation index (TPI) and vegetation index (VI) were negatively correlated with tar yield. The inorganic composition, the ash yield, which is mainly composed of SiO 2 and Al 2 O 3, reduced the tar yield. CaO and MgO in small proportions promote the tar yield of coal due to catalytic effects. The relationship between tar yield and the parameters Sr/Ba, Sr/Cu, Cu/Zn, (Cu + Mo)/Zn, U/Th, Ni/Co, δU, Ce/La, δCe, and δEu are also discussed here. It was concluded that the paleoclimate had little effect on the tar yield, while the paleoreduction conditions had an obvious influence on the tar yield. The depositional environment with higher reduction and lower salinity conditions was conducive to the increase in tar yield of coal. This work aims to accurately identify and predict tar-rich coal based on its depositional environment, which provides guidance for its exploration and development. • The tar yield of coal is significantly affected by the formation environment. • Coal facies and elemental geochemistry are important factors that affect tar yield. • The parameter thresholds were determined to recognize tar-rich coals. • Tar-rich coals always form in environments with low salinity and high reduction. [ABSTRACT FROM AUTHOR]

In this study, we successfully synthesized an innovative 1D-Zn(II) coordination polymer, denoted as [Zn(L)(H 2 O) 2 ]n, employing the Schiff base {4-[(2-hydroxy-3-methoxy-benzylidene)-amino]-benzoic acid} (H2L). The Schiff base was obtained through the reaction of 4-aminobenzoic acid and o-vanillin under slow volatilization conditions. The resulting compound exhibits remarkable green fluorescence emission properties, indicating its potential as a novel fluorescent and sensing material. Hydrogels based on hyaluronic acid (HA) and carboxymethyl chitosan (CMCS), denoted as HA/CMCS hydrogels, were synthesized using a chemical method. Additionally, we utilized bleomycin as a model drug to synthesize a novel bleomycin metal gel and assessed its anti-hemangioma activity. Molecular docking simulations revealed that the Zn complex can form stable bonds with the key target, involving the methoxy and carboxyl groups on the Zn complex., (© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

The homeostasis of the gut microbiota and circadian rhythm is critical to host health, and both are inextricably intertwined with lung cancer. Although time-restricted feeding (TRF) can maintain circadian synchronization and improve metabolic disorders, the effects of TRF on the fecal microbiome, metabolome and their diurnal oscillations in lung cancer have not been discussed. We performed 16S rRNA sequencing and untargeted metabonomic sequencing of the feces prepared from models of tumor-bearing BALB/c nude mice and urethane-induced lung cancer. We demonstrated for the first time that TRF significantly delayed the growth of lung tumors. Moreover, TRF altered the abundances of the fecal microbiome, metabolome and circadian clocks, as well as their rhythmicity, in lung cancer models of tumor-bearing BALB/c nude mice and/or urethane-induced lung cancer C57BL/6J mice. The results of fecal microbiota transplantation proved that the antitumor effects of TRF occur by regulating the fecal microbiota. Notably, Lactobacillus and Bacillus were increased upon TRF and were correlated with most differential metabolites. Pathway enrichment analysis of metabolites revealed that TRF mainly affected immune and inflammatory processes, which might further explain how TRF exerted its anticancer benefits. These findings underscore the possibility that the fecal microbiome/metabolome regulates lung cancer following a TRF paradigm., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier Inc.)

Background: Diet is fundamental to maintaining and improving human health. There is ample evidence identifying the beneficial and/or harmful effects of diet on noncommunicable diseases such as obesity, diabetes mellitus, and cardiovascular disease. However, the associations of the diet to chronic venous disease has not been fully described., Methods: Data were collected through a cross-sectional survey conducted on 1,571 community-dwelling adults in 2018. Diet intake frequency was assessed using valid food group consumption frequency questionnaires. Multivariable logistic regression models were used to evaluate the association of diet with chronic venous disease., Results: In total, 857 participants were diagnosed with chronic venous disease. Those who ate soybean products daily and 4-6 days/week had a 51-31% lower risk of chronic venous disease compared with those who only occasionally consumed soybean food, respectively. Participants who consumed eggs and egg products 1-3 days/week versus those who only occasionally ate eggs showed a lower risk of chronic venous disease [odds ratio (OR) 0.542, 95% confidence interval (CI) 0.375-0.782]. Eating fried food 4-6 days each week was associated with an increased risk of chronic venous disease (OR 3.872, 95% CI 1.263-11.599) compared with those who only occasionally ate fried foods. There is a decreasing tendency of the adjusted OR for eating soybean products daily with the severity of disease [chronic venous disease (C0-C2): OR 0.575, 95% CI 0.408-0.812; chronic venous insufficiency (C3-C6): OR 0.222, 95% CI 0.114-0.435]., Conclusions: A higher frequency in the consumption of soybean products and eggs were associated with a lower risk of chronic venous disease. High level of fried food consumption was positively associated with risk of chronic venous disease. There are certain specific trends in relation to dietary consumption and severity of disease, although these trends were less strong. These associations are largely independent of other dietary and nondietary factors., (Copyright © 2022 Elsevier Inc. All rights reserved.)