Search

When a metro train passes through the shaft, it will lead to severe pressure fluctuations on surfaces of tunnel and train due to the sudden change of tunnel cross section. Based on the three-dimensional, compressible and unsteady N-S equation method, this paper simulates aerodynamic problems caused by trains when they pass through the airshafts at the speed of 140 km/h by the means of constructing four middle shaft models, one is the real shaft, the other three are the simplifications. Time delays generated on trains’ surfaces after trains pass through shafts in the three simplifications’ model, but on tunnels’ surfaces from entering portals for the different corridor sizes. The tendency of max pressure, min pressure and pressure amplitude at trains’ and tunnels’ surfaces is consistent in four kinds of models. But the different area of shafts’ corridor sizes cause the most used model gets the maximum pressure amplitude, the real model gets the minimum pressure amplitude, and the two simplifications’ are the middle.

A novel microsphere-based fluorescent sensor 1 for determination of Hg(II) in food samples has been successfully synthesized and its fluorescent sensing properties were investigated in detail. Polystyrene microsphere was innovatively surface modified by a rhodamine derivative; therefore sensor 1 was a fluorescent sensor with high polymer material properties of polystyrene and the optical properties of a fluorescent probe. Sensor 1 displayed high selectivity, good anti-interference performance, and instantaneous response to Hg(II). The fluorescence intensity of sensor 1 showed a linear response to Hg(II) in the concentration range of 0 µM to 8 µM with a detection limit of 0.439 µM. The most valuable advantage was that sensor 1 was recyclable and environmentally-friendly. This proposed sensor 1 was applied to monitor the content of Hg(II) in real food samples, such as tap water, rice, and anglerfish. The recovery range of tap water was from 92.60 to 105.80%, the recovery range of rice was from 90.45 to 106.10%, and the recovery range of anglerfish was from 91.30 to 105.84%. The relative error was below 10% in spiked recovery studies, suggesting that fluorescent sensor 1 provides a simple, efficient, and promising method for determination of Hg(II) in complex matrices.

A novel, functional-group-tolerant, and highly regioselective one-pot synthesis of six 4-chloro-1-aryl-3-oxypyrazoles, 8a–8f, containing an oximino ester moiety has been developed. Their structures were characterized by 1H- and 13C-NMR, IR, MS, and elemental analyses. The regioselectivity of the reaction was also determined by single-crystal X-ray diffraction analysis of product 8d. The reaction pathway, proposed with the aid of DFT calculations, likely proceeds via a DMF-catalyzed mechanism, which involves an electrophilic attack by SOCl2 and two nucleophilic substitutions by benzyl bromide (BnBr) and Cl−, respectively, as the key steps. A preliminary in vitro bioassay indicated that most compounds exhibited good fungicidal activities against Sclerotinia sclerotiorum and Gibberella zeae. Especially, 8d and 8e displayed higher or similar fungicidal activities compared with pyraclostrobin at the concentration of 10 μg/ml.

Using structured mesh to discretize the calculation region, the wind velocity and pressure distribution in front of the wind barrier under different embankment heights are investigated based on the Detached Eddy Simulation (DES) with standard Spalart-Allmaras (SA) model. The Reynolds number is 4.0×105 in this calculation. The region is three-dimensional. Since the wind barrier and trains are almost invariable cross-sections, only 25 m along the track is modeled. The height of embankment ranges from 1 m to 5 m and the wind barrier is 3 m high. The results show that the wind speed changes obviously before the wind barrier on the horizontal plane, which is 4.5 m high above the track. The speed of wind reduces gradually while approaching the wind barrier. It reaches the minimum value at a distance about 5 m before the wind barrier, and increases dramatically afterwards. The speed of wind at this location is linear with the speed of far field. The train aerodynamic coefficients decrease sharply with the increment of the embankment height. And they take up the monotonicity. Meanwhile, when the height increases from 3 m to 5 m, they just change slightly. It is concluded that the optimum anemometer location is nearly 5 m in front of the wind barrier.

Six novel methyl( Z)-2-(4-{[(1-aryl-1 H-pyrazol-3-yl)oxy]methyl}phenyl) 2-(methoxyimino)acetate were synthesised by the reaction of 1-aryl-1 H-pyrazol-3-ols with methyl ( Z)-2-[4-(bromomethyl)phenyl]-2-(methoxyimino)acetate. The structure of methyl ( Z)-2-[4-({[1-(4-chlorophenyl)-1 H-pyrazol-3-yl]oxy}methyl)phenyl]-2-(methoxyimino)acetate was also determined by single-crystal X-ray diffraction analysis. A preliminary in vitro bioassay indicated that compounds 3-chlorophenyl, 4-chlorophenyl and 4-fluoro-3-(trifluoromethyl) derivatives exhibited moderate fungicidal activity against Rhizoctonia solani at a dosage of 10 μg mL-1.

Six novel chloro-containing 1-aryl-3-oxypyrazoles TMa-TMf with an oximino ester or an oximino amide moiety were prepared by the reaction of 1-aryl-1H-pyrazol-3-ols with benzyl bromide. Their structures were characterized by 1 H-NMR, 13 C-NMR, IR, MS, and elemental analysis. A preliminary in vitro bioassay indicated that compounds TMa, TMe and TMf displayed excellent fungicidal activity against Rhizoctonia solani and could be used as potential lead compounds for further development of novel fungicides.

The aryloxypyrazole structure is present in a number of bioactive molecules. Four 1,5-diaryl-3-oxypyrazoles containing benzoyl (I), thiazolidinethione (II and III) or per-O-acetylated glucopyranosyl (IV) moieties were characterized by single-crystal X-ray diffraction. Compounds I and II crystallize in a triclinic P-1 system, whereas III and IV crystallize in an orthorhombic Pbca and a monoclinic P21 space groups, respectively. The dihedral angles between the two benzene rings of the pyrazole are 61.33° (I), 62.87° (II), 57.09° (III) and 70.25° (IV). The structures were stabilized by classical intra- (C-H···S for II and III, C-H···O for IV) and intermolecular (C-H···O for I and IV) H-bonds, as well as intermolecular C-H···π stacking interactions. The theoretical FTIR results showed good agreement with the experimental data. Compounds IV, II and III showed moderate fungicidal activity against Sclerotinia sclerotiorum and Gibberella zeae. The structure-activity relationships were discussed.

Anemometers are usually set up along railway embankments to monitor wind speeds, and the layout for them has to be investigated. This work used an improved delayed detached eddy simulation (IDDES) approach to explore the flow properties around railway embankments, and then a proposal was put forward for the layout of anemometers. The numerical method was validated against previous wind tunnel tests on the speed-up ratios of the flow around a 1/300 scale two-dimensional embankment with the slope gradient of 1:2. The effects of inlet velocity profiles, i.e., uniform velocity and atmospheric boundary layer velocity profiles, on the speed-up ratios around a 5 m high railway embankment were compared. The study indicates that using a uniform velocity profile to assess the operational safety of trains running across strong wind regions could be favourable, especially when complex local terrains contribute to different wind characteristics. The anemometers should be set upstream, i.e., at a well defined distance in locations with sufficient extent of open ground and on the electrification masts along railway lines. This is not in line with the anemometer layout of the existing SWEWS (Strong Wind Early Warning Systems), the difference being due to the speed-up effect of the railway embankment, which is usually not considered explicitly. Formulas have been developed on the basis of regression of the simulation results to express the relationships between e.g. top wind speed over the embankment (located in an area where it is not possible to install anemometers) and measured wind speeds. In this way it is possible to take into account the speed-up effect encountered by the wind passing over the embankment, which needs to be considered in the operational rules in order to ensure safe operations.

HpaXm produced by the cotton leaf blight bacterium Xanthomonas citri subsp. malvacearum is a novel harpin elicitor of the induced hypersensitive response (HR) in tobacco. We investigated whether fragments of HpaXm, compared with fragments of Hpa1Xoo, are sufficient for HR or plant growth promotion (PGP) elicitation using four synthetic peptides (HpaXm35-51, HpaXm10-39, Hpa1Xoo36-52 and Hpa1Xoo10-40). We also heated the fragments to determine the heat tolerance of the functional fragments. HpaXm35-51 and Hpa1Xoo36-52 induced hypersensitive response (HR). Bursts of reactive oxygen intermediates (ROI) induced by HpaXm35-51 and Hpa1Xoo36-52 were earlier and stronger than those induced by HpaXm and Hpa1Xoo. In plants treated with HpaXm35-51 or Hpa1Xoo36-52, the expression of the HR marker genes Hin1 and Hsr203J and the active oxygen metabolism related gene AOX were significantly upregulated. These findings suggest that the predicted α-helical structures of the HpaXm35-51 and Hpa1Xoo36-52 fragments are crucial for HR. PGP result by soaking seeds in unheated/heated HpaXm10-39 or Hpa1Xoo10-40 solution prior to transfer, which obviously enhances root growth and the aerial parts of plants. The PGP related gene NtEXP6 was greatly enhanced when plants were sprayed with a solution of HpaXm10-39 or Hpa1Xoo10-40; heated fragment treatments induced higher levels of NtEXP6 expression than unheated HpaXm fragments. In addition, HR marker genes induced by the heated fragments had lower expression levels than when induced with unheated HpaXm fragments. Moreover, the expression levels of HR marker genes and PGP related genes induced by treatment with Hpa1Xoo fragments before or after heating were the opposite of those induced by HpaXm fragments. Different functional fragments of harpin and different harpins with the same functional region have different degrees of heat tolerance. Therefore, the heat resistance of harpin is conservative, but the degree of heat tolerance of the functional fragments is specific.

An alternative type of controllable underwater towed system is proposed. The towed vehicle in the system is composed of an adjustable depressing wing and a rectangular wing-shaped main body. Laboratory experimental observations on the behavior of this kind of towed system are reported and the experimental techniques to detect the hydrodynamic performances of the system are described. The results of the study indicate that with such a design the objective of rapid trajectory manipulation and relative stable attitude towing for the towed vehicle can be achieved; the experimental monitoring approaches provide an effective and reliable way to detect the hydrodynamic behavior of a towed system model tested in a ship model towing tank which would be quite useful in understanding the hydrodynamic and control behavior of the system.

A series of novel 1,4-dihydro-2,6- dimethyl-3,5-pyridinedicarboxamides were synthesized and characterized by infrared absorption spectrum (IR), proton nuclear magnetic resonance (1H NMR), elemental analysis, ultraviolet spectrum (UV), and fluorescence techniques, together with X-ray single crystal diffraction. The results of density functional theory (DFT) and time-dependent density functional theory (TDDFT) calculations provided a reasonable explanation on the molecular structures, the molecular frontier orbital, and the spectra of electronic absorption and emission. The present work will be helpful to systematically understanding of the structures and the optical properties of 1,4-dihydropyridines for studying the structure-activity relationship and to develop new drugs and their analytical methods.

HER2/Neu is overexpressed in 25-30% of all human breast cancers as a result of both gene amplification and enhanced transcription. Transcriptional upregulation of HER2/neu leads to a 6-8-fold increased abundance of its mRNA per gene copy and likely results from the elevated activity of transcription factors acting on the HER2/neu promoter. Here we report that transcripts of PEA3, an ETS transcription factor implicated in oncogenesis, were increased in 93% of HER2/Neu-overexpressing human breast tumor samples. Analyses to uncover the molecular basis for elevated PEA3 transcripts in HER2/Neu-positive breast tumors revealed that the HER2/Neu receptor tyrosine kinase initiated an intracellular signaling cascade resulting in increased PEA3 transcriptional activity; transcriptionally-activated PEA3 stimulated HER2/neu and PEA3 gene transcription by binding to sites in the promoters of these genes. PEA3 also activates transcription of genes encoding matrix-degrading proteinases, enzymes required for tumor cell migration and invasion. These findings implicate PEA3 in the initiation and progression of HER2/Neu positive breast cancer, and suggest that PEA3 and signaling proteins affecting its regulation are appropriate therapeutic targets.

Promoter elements accounting for HER2 (c-erbB-2/neu) overexpression were searched for in several human breast cancer cell lines (MDA-453, BT-474, ZR-75-1, MCF-7) known to express constitutively a 30-fold range in HER2 transcripts per gene copy. HER2 overexpressing cells showed a single prominent DNase I hypersensitive site near a conserved and hitherto unrecognized ets response element (GAGGAA), located 38 bases down-stream from the CAAT box and directly 5' of the TATA box in the human HER2 promoter. Transient transfection of HER2 promoter constructs (0.125, 0.5, and 2.0 kilobase pairs (kb)) demonstrated that the most proximal promoter region (0.125 kb) was capable of conferring up to 30-fold enhanced activity in HER2-overexpressing cell lines relative to low HER2-expressing control lines. Site-directed mutagenesis of the ets response element (GAGGAA-->GAGAGA) caused a > or = 60% reduction in promoter activity affecting at least 0.5 kb of upstream HER2 regulatory sequence. Gel-shift assays with nuclear extracts and oligonucleotide sequences spanning the 0.125-kb promoter region detected an ETS-immunoreactive complex, present most abundantly in cells overexpressing HER2, whose high-affinity binding depended on the GAGGAA response element. Methylation interference confirmed the ETS-specific pattern of protein binding by this complex to guanine bases in the ets response element. UV cross-linking and immunoprecipitation implicate a approximately 60-kDa ETS protein, and candidate ETS genes expressed in these breast cancer cells include GABP alpha, elk-1, elf-1, and PEA3.

The hydrodynamic and control performances of a self-stable controllable underwater towed vehicle developed by South China University of Technology under different depth trajectory control operations are analyzed by means of a proposed hydrodynamic numerical model. The model is established based on LMBP algorithm of neural network theory. Training samples for the neural network model are provided from the experimental data of the vehicle prototype towing experiments conducted in a large-scale ship model towing tank under the manipulation of a depressing wing installed in the vehicle. After the LMBP model is established, a depth trajectory control system for the towed vehicle is designed in order to accomplish vehicle trajectory control. This system is mainly composed of tow parts: a neural network identifier based on genetic algorithm and a fuzzy neural network controller based on genetic algorithm simulated annealing. Hydrodynamic performances of the vehicle under various control operations can then be numerically simulated with the proposed LMBP model and the depth trajectory control system of the towed vehicle. In numerical simulation of trajectory control to the towed vehicle, deflection of the vehicle’s depressing wing is adjusted at every time step by the proposed control system to match the trajectory of the vehicle with a pre-designated one. The value of the deflection is taken as input parameter for the LMBP neural network model, trajectory and attitude behavior of the towed vehicle under the control manipulations can then be predicted by the LMBP model.Copyright © 2006 by ASME

The >30 known members of the Ets multigene family of transcriptional regulators are increasingly being recognized for their involvement in early embryonic development and late tissue maturation, directing stage-specific and tissue-restricted programs of target gene expression. Identifiable primarily by their 85 amino acid ETS DNA-binding domain and dispersed across all metazoan lineages into distinct subfamilies, Ets genes also produce malignancies in humans and other vertebrates when overexpressed or rearranged into chimeras retaining the ETS domain, suggesting that their oncogenic potential is determined by the program of target genes they regulate. Searching for Ets factors that regulate expression of the HER2/neu (c-erbB2) oncogene in human breast cancer, we identified a new epithelium-restricted Ets encoding an ETS domain homologous to the Drosophila E74/human Elf-1 subfamily, an amino-terminal region (A-region or Pointed domain) homologous to the distantly related Ets-1 subfamily, and a serine-rich box homologous to the transactivating domain of the lymphocyte-restricted High Mobility Group (HMG) protein, SOX4. Recombinant protein encoded by ESX (for epithelial-restricted with serine box) exhibits Ets-like DNA binding specificity in electrophoretic mobility shift assays and, in transient transfection assays, transactivates Ets-responsive promoter elements including that found in the HER2/neu oncogene. ESX is located at chromosome 1q32 in a region known to be amplified in 50% of early breast cancers, is heregulin-inducible and overexpressed in HER2/neu activated breast cancer cells. Tissue hybridization suggests that ESX becomes overexpressed at an early stage of human breast cancer development known as ductal carcinoma in situ (DCIS).

The title compound, C20H17N3O2S2, was synthesized by the reaction of 2-(1,5-diphenyl-1H-pyrazol-3-yloxy)acetic acid and thiazolidine-2-thione. The C-linked benzene ring, N-linked benzene ring and thiazolidine-2-thione ring are twisted 31.33 (2), 62.87 (1) and 82.71 (2)°, respectively, from the plane of the bridging 1H-pyrazole ring. The phenyl rings are oriented at a dihedral angle of 72.16 (2)°.