Search

Dipteronia (order Sapindales) is an endangered genus endemic to China and has two living species, D. sinensis and D. dyeriana. The plants are closely related to the genus Acer, which is also classified in the order Sapindales. Evolutionary studies on Dipteronia have been hindered by the paucity of information on their genomes and plastids. Here, we used next generation sequencing to characterize the transcriptomes and complete chloroplast genomes of both Dipteronia species. A comparison of the transcriptomes of both species identified a total of 7,814 orthologs. Estimation of selection pressures using Ka/Ks ratios showed that only 30 of 5,435 orthologous pairs had a ratio significantly greater than 1, i.e., showing positive selection. However, 4,041 orthologs had a Ka/Ks < 0.5 (p < 0.05), suggesting that most genes had likely undergone purifying selection. Based on orthologous unigenes, 314 single copy nuclear genes were identified. Through a combination of de novo and reference guided assembly, plastid genomes were obtained; that of D. sinensis was 157,080 bp and that of D. dyeriana was 157,071 bp. Both plastid genomes encoded 87 protein coding genes, 40 tRNAs, and 8 rRNAs; no significant differences were detected in the size, gene content, and organization of the two plastomes. We used the whole chloroplast genomes to determine the phylogeny of D. sinensis and D. dyeriana and confirmed that the two species were highly divergent. Overall, our study provides comprehensive transcriptomic and chloroplast genomic resources, which will be valuable for future evolutionary studies of Dipteronia.

We present a systematic MEMS structural design approach via a "trial-and-error"learning process by using the deep reinforcement learning framework. This scheme incorporates the feedback from each "trial"to obtain sophisticated strategies for MEMS design optimizations. Disk-shaped MEMS resonators are selected as case studies and three remarkable advancements have been realized: 1) accurate overall performance predictions (97.9%) via supervised learning models; 2) efficient MEMS structural optimizations to guarantee targeted structural properties with an excellent generation accuracy of 97.7%; and 3) superior design explorations to achieve one order of magnitude performance enhancement than the training dataset. As such, the proposed scheme could facilitate a wide spectrum of MEMS applications with this data-driven inverse design methodology.

This work presents an air-coupled piezoelectric micromachined ultrasonic transducer (pMUT) with high transmitting acoustic pressure by using sputtered potassium sodium niobate (K,Na)NbO3 (KNN) thin film with a high piezoelectric coefficient (e31 ∼ 8-10 C/m2) and low dielectric constant (ϵr ∼ 260-300) for the first time. The fabricated KNN pMUT with a resonant frequency at 104.5 kHz has been tested to exhibit unprecedented results: (1) high sound pressure level (SPL) of 109 dB/V at a distance of 10 cm, which is 8 times higher than that of AlN-based pMUTs at a similar frequency; (2) low-voltage operation of only 4 volts peak-to-peak amplitude (Vp-p); and (3) good receiving sensitivity. As such, this work presents a new class of high-SPL and low-driving-voltage pMUTs for potential applications in various fields, including consumer electronics, such as but not limited to haptic feedback, loudspeaker, and AR/VR systems.

This paper reports a low-frequency piezoelectric micromachined ultrasonic transducer (pMUT) with a small attenuation coefficient to realize the long-distance range finding applications in air. Pulse-detection measurements of one pair of pMUT devices show a >6-meter traveling distance in air under a 37.32 kHz driving frequency. As an example, two pMUT chips are mounted on two drones as the transceiver and receiver, respectively. Measurements of the separation distance are conducted based on the time-of-flight (ToF) principle with up to 32 fps (frames per second) for real-time detections. This demo of drone-mounted pMUT system illustrates the advantages of pMUT in terms of compactness and low power consumption for applications in drones including obstacle avoidance, inter collision prevention, aerial coordination, and acoustic-based vision.

This work reports an engineered platform for the non-contact haptic stimulation on human skins by means of an array of piezoelectric micromachined ultrasonic transducer (pMUT) via the beamforming scheme. Compared to the state-of-art reports, three distinctive achievements have been demonstrated: (1) individual single pMUT unit based on lithium niobate (LN) with measured high SPL (sound pressure level) of 133 dB at 2 mm away; (2) a beamforming scheme simulated and experimentally proved to generate ∼2.3x higher pressure near the focal point; and (3) the combination of auto-positioning and haptic stimulations on volunteers with the smallest reported physical device size to achieve haptic sensations. As such, this work could have practical applications in the broad areas to stimulate haptic sensations, such as AR (Augmented Reality), VR (Virtual Reality), and robotics.

This paper presents a mid-air thermal interface enabled by a piezoelectric micromachined ultrasonic transducer (pMUT) array. The two-stage thermal actuating process consists of an ultrasound-transmission process via a pMUT array and an ultrasound-absorption process via porous fabric. The pMUT design employs sputtered potassium sodium niobate (K, Na)NbO3 (KNN) thin film with a high piezoelectric coefficient ( 31 ~ 8-10 C/m2) as piezoelectric layer for enhanced acoustic pressure. Testing results show that the prototype pMUT array has a resonant frequency around 97.6 kHz, and it can generate 1970 Pa of focal pressure at 15 mm away under the 10.6 Vp-p excitation. As a result, fabric temperature in the central focal area can rise from 24.2°C to 31.7°C after 320 seconds with an average temperature variation rate of 0.023°C /s. Moreover, thermal sensations on the human palms have been realized by the heat conduction through the fabric-skin contact. As such, this work highlights the promising application of pMUT array with high acoustic pressure for human-machine interface, particularly mid-air thermal display.

Myelodysplastic syndromes (MDS) are characterized by impaired proliferation and differentiation of hematopoietic stem cells. The participation of toll-like receptor (TLR)-mediated signaling in MDS is well documented. Increased TLR signaling leads to the constitutive activation of NF-κB, which mediates inflammation, cell proliferation and apoptosis. In addition, the TLR pathway induces the expression of miRNAs which participate in the fine-tuning of the inflammatory response. miRNAs also regulate other biological processes, including hematopoiesis. miR-125a and miR-125b are known modulators of hematopoiesis and are abnormally expressed in several hematologic malignancies. However, little is known about their role in MDS. NF-κB-activating ability has been described for both miRNAs. We studied the role of miR-125a/miR-125b in MDS and their relationship with TLR signaling and hematopoietic differentiation. Our results indicate that miR-125a is significantly overexpressed in MDS patients and correlates negatively with patient survival. Expression of miR-99b, which is clustered with miR-125a, is also directly correlated with prognosis of MDS. Both miR-125a and miR-99b activated NF-κB in vitro; however, we observed a negative correlation between miR-99b expression and the levels of TLR2, TLR7 and two downstream genes, suggesting that NF-κB activation by the miRNA cluster occurs in the absence of TLR signaling. We also show that TLR7 is negatively correlated with patient survival in MDS. In addition, our data suggest that miR-125a may act as an NF-κB inhibitor upon TLR stimulation. These results indicate that miR-125a is involved in the fine-tuning of NF-κB activity and that its effects may depend on the status of the TLR pathway. Furthermore, we observed that miR-125a inhibits erythroid differentiation in leukemia and MDS cell lines. Therefore, this miRNA could serve as a prognostic marker and a potential therapeutic target in MDS.

MYD88 is a key mediator of Toll-like receptor innate immunity signaling. Oncogenically active MYD88 mutations have recently been reported in lymphoid malignancies, but has not been described in MDS. To characterize MYD88 in MDS, we sequenced the coding region of the MYD88 gene in 40 MDS patients. No MYD88 mutation was detected. We next characterized MYD88 expression in bone marrow CD34+ cells (N = 64). Increased MYD88 RNA was detected in 40% of patients. Patients with higher MYD88 expression in CD34+ cells had a tendency for shorter survival compared to the ones with lower MYD88, which was significant when controlled for IPSS and age. We then evaluated effect of MYD88 blockade in the CD34+ cells of patients with lower-risk MDS. Colony formation assays indicated that MYD88 blockade using a MYD88 inhibitor resulted in increased erythroid colony formation. MYD88 blockade also negatively regulated the secretion of interleukin-8. Treatment of MDS CD34+ cells with an IL-8 antibody also increased formation of erythroid colonies. These results indicate that MYD88 plays a role in the pathobiology of MDS and may have prognostic and therapeutic value in the management of patients with this disease.

The Notch pathway can have both oncogenic and tumor suppressor roles, depending on cell context. For example, Notch signaling promotes T cell differentiation and is leukemogenic in T cells, whereas it inhibits early B cell differentiation and acts as a tumor suppressor in B cell leukemia where it induces growth arrest and apoptosis. The regulatory mechanisms that contribute to these opposing roles are not understood. Aberrant promoter DNA methylation and histone modifications are associated with silencing of tumor suppressor genes and have been implicated in leukemogenesis. Using methylated CpG island amplification (MCA)/DNA promoter microarray, we identified Notch3 and Hes5 as hypermethylated in human B cell acute lymphoblastic leukemia (ALL). We investigated the methylation status of other Notch pathway genes by bisulfite pyrosequencing. Notch3, JAG1, Hes2, Hes4 and Hes5 were frequently hypermethylated in B leukemia cell lines and primary B-ALL, in contrast to T-ALL cell lines and patient samples. Aberrant methylation of Notch3 and Hes5 in B-ALL was associated with gene silencing and was accompanied by decrease of H3K4 trimethylation and H3K9 acetylation and gain of H3K9 trimethylation and H3K27 trimethylation. 5-aza-2'-deoxycytidine treatment restored Hes5 expression and decreased promoter hypermethylation in most leukemia cell lines and primary B-ALL samples. Restoration of Hes5 expression by lentiviral transduction resulted in growth arrest and apoptosis in Hes5 negative B-ALL cells but not in Hes5 expressing T-ALL cells. These data suggest that epigenetic modifications are implicated in silencing of tumor suppressor of Notch/Hes pathway in B-ALL.

Dipteronia Oliver (Aceraceae) is an endangered Chinese endemic genus consisting of two living species, Dipteronia sinensis and Dipteronia dyeriana. However, studies on the population genetics and evolutionary analyses of Dipteronia have been hindered by limited genomic resources and genetic markers. Here, the generation, de novo assembly and annotation of transcriptome datasets, and a large set of microsatellite or simple sequence repeat (SSR) markers derived from Dipteronia have been described. After Illumina pair-end sequencing, approximately 93.2 million reads were generated and assembled to yield a total of 99,358 unigenes. A majority of these unigenes (53%, 52,789) had at least one blast hit against the public protein databases. Further, 12,377 SSR loci were detected and 4179 primer pairs were designed for experimental validation. Of these 4179 primer pairs, 435 primer pairs were randomly selected to test polymorphism. Our results show that products from 132 primer pairs were polymorphic, in which 97 polymorphic SSR markers were further selected to analyze the genetic diversity of 10 natural populations of Dipteronia. The identification of SSR markers during our research will provide the much valuable data for population genetic analyses and evolutionary studies in Dipteronia.

In the present study, the CA III and IV autoantibodies, CA activity, antioxidant enzymes and cytokines in rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), diabetes, hypertensive renal disease, and heart failure were investigated. The anti-CA III antibody titers in patients with RA, SLE, and type 1 diabetes (T1D) were significantly higher than that in control groups (P