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High-fat diet (HFD)-induced insulin resistance (IR) in skeletal muscle is often accompanied by mitochondrial dysfunction and oxidative stress. Boosting nicotinamide adenine dinucleotide (NAD) using nicotinamide riboside (NR) can effectively decrease oxidative stress and increase mitochondrial function. However, whether NR can ameliorate IR in skeletal muscle is still inconclusive. We fed male C57BL/6J mice with an HFD (60% fat) ± 400 mg/kg·bw NR for 24 weeks. C2C12 myotube cells were treated with 0.25 mM palmitic acid (PA) ± 0.5 mM NR for 24 h. Indicators for IR and mitochondrial dysfunction were analyzed. NR treatment alleviated IR in HFD-fed mice with regard to improved glucose tolerance and a remarkable decrease in the levels of fasting blood glucose, fasting insulin and HOMA-IR index. NR-treated HFD-fed mice also showed improved metabolic status regarding a significant reduction in body weight and lipid contents in serum and the liver. NR activated AMPK in the skeletal muscle of HFD-fed mice and PA-treated C2C12 myotube cells and upregulated the expression of mitochondria-related transcriptional factors and coactivators, thereby improving mitochondrial function and alleviating oxidative stress. Upon inhibiting AMPK using Compound C, NR lost its ability in enhancing mitochondrial function and protection against IR induced by PA. In summary, improving mitochondrial function through the activation of AMPK pathway in skeletal muscle may play an important role in the amelioration of IR using NR.

Background Stress granules formation is closely associated with the progression of hepatocellular carcinoma (HCC). Factors determination this process remain to be elucidated. In this study, stress granule-related genes were validated as a predictor of HCC. Methods The stress granules-related associated genes were collected from the MSGP database and the MsigDB database. A novel prognostic risk scoring model were constructed by paired gene signature method. We identified eukaryotic translation initiation factor 4A3 (EIF4A3) and karyopherin subunit alpha 2 (KPNA2) as candidate prognostic biomarkers, and their correlation with both prognosis and immune infiltration in HCC were evaluated. The expression of EIF4A3 and KPNA2 in HCC tissues was detected through immunohistochemistry (IHC). Results Through pairing of all DESG gene, we obtained a total of 16251 significance pairs. Subsequently, 93 pairs of all pairs containing EIF4A3 were extracted in this research. As a stress granule formation regulator, KPNA2 displayed the greatest correlations with EIF4A3 in HCC. Hyperactivated EIF4A3 and KPNA2 is associated with the poor clinical outcome of HCCs after hepatic resection. Involvement of EIF4A3 and KPNA2 in immune infiltration have been showed. Conclusion Our study identified coexistence of EIF4A3 and KPNA2 dysregulation inform poor clinical outcomes in HCC.

The West Qinling Orogen (WQO), which is bounded by the Qilian Orogenic Belt, Qaidam Block and the Songpan-Ganzi Block, is the western extension of the Qinling Orogenic Belt, and experienced complex tectonic evolution processes, involving the opening, subduction and closure history of the Proto- and Paleo-Tethys Oceans. The WQO features widespread Indosinian magmatic rocks, which are crucial to constrain the tectonic evolution of the WQO. The Indosinian magmatic rocks were formed mainly in two stages, 250 to 240 Ma and 225 to 210 Ma. The Early Indosinian magmatic rocks (250 to 240 Ma) are mainly distributed in the west and middle northern WQO. In comparison, the Late Indosinian magmatic rocks are mainly exposed in the eastern WQO, but also in the western WQO and the Bikou terrane. Controversy has existed for a long time on the petrogenesis and tectonic setting of the Early Indosinian magmatic rocks. We selected four respective plutons, including the Heimahe pluton, the Ren’ai pluton, the Daerzang pluton and the Ganjiagongma pluton. Detailed field investigation, petrology, LA-ICP-MS zircon U-Pb dating, zircon Lu-Hf isotope analyses, whole rock geochemistry and Sr-Nd isotope analyses, and mineral EPMA analyses were conducted for the studied plutons. The studied plutons were emplaced between 246 to 241 Ma according to zircon U-Pb dating results. Based on detailed studies on petrology, geochronology and geochemistry, we emphasis the significance of magma mixing in the petrogenesis of the Early Indosinian granitic rocks. The high Mg# signature of the Early Indosinian granitic rocks were generated by magma mixing between mafic and felsic magmas, but not result of direct fractional crystallization of mafic rocks. The granitic rocks with high Sr/Y values in the WQO, represented by the Ganjiagongma pluton, were not derived from thickened continental crust. No evident continental thickening occurred in the WQO during the Early Indosinian. Combining with regional geological evidence, we propose an alternative tectonic model to explain the evolution history of the WQO during the early Mesozoic. The A’nimaque-Mianlue ocean subducted northward with low angle, then the subducted slab rolled back during the Late Permian to Middle Triassic, and the ocean closured in the Late Triassic. This model can explain the spatial and temporal distribution characteristics of the magmatic rocks and sedimentary rocks, as well as Late Triassic uplift and deformation event in the WQO.

The East Kunlun Orogen on the northern margin of the Tethyan orogenic system records a history of Gondwana dispersal and Laurasian accretion. Based on a synthesis of sedimentary, structural, lithological, geochemical, and geochronological data from the East Kunlun Orogen and adjacent regions, we discusses the spreading and northward consumption of the Paleo-Tethys Ocean during Late Paleozoic-Early Mesozoic times. The main evolutionary stages are: (1) During Carboniferous to Middle Permian, the Paleo-Tethys Ocean (Buqingshan Ocean) was in an ocean spreading stage, as suggested by the occurrence of Carboniferous MORB-, and OIB-type oceanic units and Carboniferous to Middle Permian Passive continental margin deposits; (2) The Buqingshan Ocean subducted northward beneath the East Kunlun Terrane, leading to the development of a large continental magmatic arc (Burhan Budai arc) and forearc basin between ~270-240 Ma; (3) During the late Middle Triassic to early Late Triassic (ca. 240-230 Ma), the Qiangtang terrane collided with the East Kunlun-Qaidam terranes, leading to the final closure of the Buqingshan Ocean and occurrences of minor collision-type magmatism and potentially inception of the Bayan Har foreland basin; (4) Finally, the East Kunlun Orogen evolved into a postcollisional stage and produced major magmatic flare-ups and polymetallic mineral deposits between Late Triassic to Early Jurassic (ca. 230-200 Ma), which is possibly related to asthenospheric mantle upwelling induced by delamination of thickened continental lithosphere and partial melting of the lower crust. Accordingly, we propose that the Wilson cycle-like processes controlled the Late Paleozoic-Early Triassic tectonic evolution of East Kunlun, which provides significant implications for the evolution of Paleo-Tethys Ocean.

Purpose This study aims to investigate the status of library and information science (LIS) education in Pakistan, as well as LIS progress, level of employment and issues, infrastructure and facilities, challenges faced by LIS institutions and future prospects of the profession. Design/methodology/approach A total of 115 permanent/regular and visiting academic members from different Pakistani institutions/universities participated in the research. A mixed-method was used, and data was collected through questionnaires and interviews. The data was analyzed quantitatively and qualitatively. Findings This study’s results showed that assistant, associate and associate professors have been very involved in producing high-quality research articles and producing quality graduates. This study also found that there are several problems with LIS education in Pakistan, such as a lack of qualified teachers, resources and facilities. This study provides all the answers to these problems. They are useful for LIS teachers and students in Pakistan because LIS education has been around for a long time, but there are still some problems that need to be fixed. The results could have an immediate effect on the direction, ability to survive and future of LIS education in Pakistan. Originality/value Previously, different studies have been conducted on evolution and challenges to the LIS profession, but to the best of the authors’ knowledge, no study has been reported based on the professional experiences in other Universities of Pakistan. According to the research, the findings are crucial for LIS professors and students in Pakistan. In addition, the research investigates the challenges faced by LIS teachers in Pakistan. It concludes with suggestions on how to address some of these problems. It is also said that Pakistan must develop universal standards for LIS to close the gap between what users want and what library workers can do.

Purpose The field of library and information sciences (LIS) is crucial to our educational system. Across the globe, the LIS systems operate at varying levels and rates of efficiency. One of the developing nations is Pakistan, which has LIS systems in all of its colleges, universities and schools. This multimethod study aims to identify and quantify elements that are detrimental to LIS progress as well as evaluate the faculty and infrastructure profiles of universities that offer LIS undergraduate and graduate level programmes. Design/methodology/approach Data was collected from the study's participants, who were mainly LIS professionals and faculty at 17 different universities, using survey questionnaires and in-person interviews. This study used a descriptive survey methodology, gathering information through a Google Survey and filling it out with a premade survey proforma. The survey responses were examined using content analysis. The development of LIS instructional and scholarly output is influenced by a variety of factors, which were investigated using a generalized linear model (GLM). To determine whether there was a statistically significant difference in opinion between faculty members and working professionals, as well as between men and women, the outcomes of an independent sample t-test were examined. Findings According to our data, the factors that have the biggest impact on the caliber and output of LIS research are “poor writing skills” (3.43), “lack of journal publication fees” (3.51) and “lack of research skills” (3.78). The top GLM model identified poor writing skills, a lack of publication fees and a lack of research expertise as bottleneck characteristics for producing high-quality LIS research. The aforementioned factors were 3.62, 2.41 and 2.19 times more significant than the average factor, respectively, to put it another way. Originality/value This study’s analysis revealed that there is no real distinction between the two groups' viewpoints. The results of this study can be applied to problems and challenges associated with LIS education in Pakistani educational institutions.

BACKGROUND: Meta-analysis was used to compare the difference between 3D reconstruction technology and 2D computed tomography (CT) before surgery for primary hepatic carcinoma (PHC) and to systematically evaluate the application value of 3D vascular reconstruction and 3D navigation technology in guiding precise liver resection for PHC. However, there are still many controversies in this aspect, and there are no clear conclusions on the effectiveness and safety of three-dimensional vascular reconstruction combined with three-dimensional navigation in laparoscopic hepatectomy. Therefore, it is necessary to systematically review the results of previous studies with meta method in this study to determine their clinical efficacy and complications and guide clinical treatment. METHODS: We used the Cochrane Library, PubMed, Embase, Chinese Biomedical Literature Database (CBM), China National Knowledge Infrastructure (CNKI), Chinese Science and Technology Periodicals Full-Text Database (VIP), and Wanfang database to conduct an online search for data from randomized controlled trials of preoperative 3D reconstruction versus conventional CT in hepatectomy published up to October 2021. Relevant literature was selected based on the inclusion criteria, data was extracted, and quality evaluation of the included literature was carried out. I(2) test was used to evaluate heterogeneity among the studies, and Cochrane risk of bias 2.0 was used to evaluate the studies. RESULTS: A total of 16 studies were included in this study. Meta-analysis showed that there were statistically significant differences between the 3D vascular reconstruction group and conventional surgery group in operation time [mean differences (MD) =−40.10, 95% confidence interval (CI): −74.94, −5.26, P=0.02, I(2)=78%, Z=2.26] and intraoperative blood loss (MD =−50.40, 95% CI: −62.93, −37.86, P

Previous studies have shown that the autonomic nervous system (ANS), which can be affected by emotions, is important in the occurrence or progression of glaucoma. The autonomic innervation distributed in the anterior chamber (AC) structures might play an efferent role in the neural regulation of intraocular pressure (IOP). This study aimed to investigate the anatomic neural connection from the emotional brain to autonomic innervation in the AC.A retrograde trans-multisynaptic pseudorabies virus encoded with an enhanced green fluorescent protein (PRV531) and non-trans-synaptic tracer FAST Dil were injected into the right eye of mice, respectively. Fluorescent localization in the emotional brain and preganglionic nuclei was studied. Five and a half days after PRV531 injection into the right AC, fluorescent signals were observed in several emotional brain regions, including the amygdala, agranular insular cortex, lateral septal nuclei, periaqueductal gray, and hypothalamus. Autonomic preganglionic nuclei, including Edinger-Westphal nucleus, superior salivatory nucleus, and intermediolateral nucleus, were labeled using PRV531.The sensory trigeminal nuclei were not labeled using PRV531. The fluorescence signals in the nuclei mentioned above showed bilateral distribution, primarily on the ipsilateral side. Seven days after injecting FAST Dil into the AC, we observed no FAST Dil-labeled neurons in the central nervous system.Our results indicate a neural connection from the emotional brain to autonomic innervation in the AC, which provides anatomical support for the emotional influence of IOP via the ANS.

Advanced Patch Attacks (PAs) on object detection in natural images have pointed out the great safety vulnerability in methods based on deep neural networks. However, little attention has been paid to this topic in Optical Remote Sensing Images (O-RSIs). To this end, we focus on this research, i.e., PAs on object detection in O-RSIs, and propose a more Threatening PA without the scarification of the visual quality, dubbed TPA. Specifically, to address the problem of inconsistency between local and global landscapes in existing patch selection schemes, we propose leveraging the First-Order Difference (FOD) of the objective function before and after masking to select the sub-patches to be attacked. Further, considering the problem of gradient inundation when applying existing coordinate-based loss to PAs directly, we design an IoU-based objective function specific for PAs, dubbed Bounding box Drifting Loss (BDL), which pushes the detected bounding boxes far from the initial ones until there are no intersections between them. Finally, on two widely used benchmarks, i.e., DIOR and DOTA, comprehensive evaluations of our TPA with four typical detectors (Faster R-CNN, FCOS, RetinaNet, and YOLO-v4) witness its remarkable effectiveness. To the best of our knowledge, this is the first attempt to study the PAs on object detection in O-RSIs, and we hope this work can get our readers interested in studying this topic.

Objective. To investigate a rapid and effective method for the examination of coronary CTA in emergency patients requiring coronary CTA examination, who have faster heart rate (≧80 bpm) or cannot cooperate with the examination due to the inability of breath holding at poor physical conditions. Methods. Before coronary CTA examination, with the ECG monitoring, intravenous injection of esmolol was given to achieve rapid heart rate reduction. Without the patient’s cooperation, coronary CTA examination was then performed in a quick and effective manner using the 640-slice high-speed CT. The diagnosis report was obtained through the subsequent reconstruction analysis using artificial intelligence software. Conclusion. Esmolol injection can rapidly reduce the heart rate of normal people during exercise and at rest, and the steady blood concentration can be reached in 2 minutes. The half-life is about 5 minutes, with short duration and fewer side effects on patients. The diagnostic rate of coronary artery segment using (excellent + good) CTA image of the patients with esmolol and artificial intelligence analysis in the experimental group was 95.4%, while the diagnostic rate was 91.1% in the control group, and there was no significant statistical difference between the two groups ( P > 0.05 ). Esmolol injection can rapidly reduce heart rate in patients with high heart rate, without holding breath or long-term preoperative preparation; the combination with the analysis of subsequent artificial intelligence reconstruction is a new method for rapid and effective coronary CTA examination in all patients.

The bias and gate voltages can tune different spin-polarized transport properties in novel four-terminal MoS2/V7(Bz)8 and graphene/V7(Bz)8 vdW junctions.

The deformation of arch dam during impoundment is critical to evaluate the safety of arch dam, which is influenced by many factors that have different action mechanisms. After the monitoring data at different impoundment stages were comprehensively analyzed, a finite element simulation analysis model that can consider the real temperature, stress and deformation of the dam was set up with the help of simulation analysis method based on the whole process of the dam, which can effectively separate the single deformation caused by water pressure, dead weight, creep, air temperature, seepage and valley deformation. Then, the deformation mechanism of arch dam was gotten and the weight of each deformation influencing factor was accurately defined, thus providing experience and reference for the deformation law analysis of similar projects.

In this paper, the dam of Baihetan Hydropower Station was taken as an example to establish a refined three-dimensional finite element model (FEM) of the dam-dam foundation based on the comprehensive analysis of the safety monitoring data of the dam during the whole life cycle and the simulation analysis for the whole process of the whole dam, so as to accurately understand the working behavior of the dam during impounding process, to do a feedback analysis on the influence mechanism of the overall safety and deformation of the arch dam in the whole process of construction, impoundment and operation, and to predict and evaluate the potential safety risks of different water levels in the whole life cycle of the arch dam, thus providing a scientific basis for dam impounding safety and regulation decision-making.

This study focused on an ozone pollution event occurring in winter (January) in Guangzhou. Various influencing factors were analyzed, including various atmospheric trace gases, meteorological conditions during the whole pollution process, as well as the characteristics of the main O