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The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first identified in China by the end of 2019 and was responsible for a pandemic in the human population that resulted in millions of deaths worldwide. Since the beginning of the pandemic, the role of animals as spill-over or reservoir hosts was discussed. In addition to cats and dogs, ferrets are becoming increasingly popular as companion animals. Under experimental conditions, ferrets are susceptible to SARS-CoV-2 and it appears that they can also be infected through contact with a SARS-CoV-2 positive owner. However, there is still little information available regarding these natural infections. Here, we serologically tested samples collected from pet ferrets (n = 45) from Poland between June and September 2021. Of the ferrets that were included in the study, 29% (13/45) had contact with owners with confirmed SARS-CoV-2 infections. Nevertheless, SARS-CoV-2-specific antibodies could not be detected in any of the animals, independent of the infection status of the owner. The obtained results suggest that ferrets cannot be readily infected with SARS-CoV-2 under natural conditions, even after prolonged contact with infected humans. However, due to the rapid mutation rate of this virus, it is important to include ferrets in future monitoring studies. [ABSTRACT FROM AUTHOR]

Over the last 20 years, begomoviruses have emerged as devastating pathogens, limiting the production of different crops worldwide. Weather conditions increase vector populations, with negative effects on crop production. In this work we evaluate the relationship between the incidence of begomovirus and weather before and during the crop cycle. Soybean and bean fields from north-western (NW) Argentina were monitored between 2001 and 2018 and classified as moderate (≤50%) or severe (>50%) according to the begomovirus incidence. Bean golden mosaic virus (BGMV) and soybean blistering mosaic virus (SbBMV) were the predominant begomovirus in bean and soybean crops, respectively. Nearly 200 bio-meteorological variables were constructed by summarizing climatic variables in 10-day periods from July to November of each crop year. The studied variables included temperature, precipitation, relative humidity, wind (speed and direction), pressure, cloudiness, and visibility. For bean, high maximum winter temperatures, low spring humidity, and precipitation 10 days before planting correlated with severe incidence. In soybeans, high temperatures in late winter and in the pre-sowing period, and low spring precipitations were found to be good predictors of high incidence of begomovirus. The results suggest that temperature and pre-sowing precipitations can be used to predict the incidence status [predictive accuracy: 80% (bean) and 75% (soybean)]. Thus, these variables can be incorporated in early warning systems for crop management decision-making to reduce the virus impact on bean and soybean crops. [ABSTRACT FROM AUTHOR]

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Venous thromboembolism (VTE) is known to be a common respiratory and/or cardiovascular complication in hospitalized patients with viral infections. Numerous studies have proven human immunodeficiency virus infection to be a prothrombotic condition. An elevated VTE risk has been observed in critically ill H1N1 influenza patients. VTE risk is remarkably higher in patients infected with the Hepatitis C virus in contrast to uninfected subjects. The elevation of D‐dimer levels supported the association between Chikungunya and the Zika virus and the rise of clinical VTE risk. Varicella‐zoster virus is a risk factor for both cellulitis and the consequent invasive bacterial disease which may take part in thrombotic initiation. Eventually, hospitalized patients infected with the coronavirus disease of 2019 (COVID‐19), the cause of the ongoing worldwide pandemic, could mainly suffer from an anomalous risk of coagulation activation with enhanced venous thrombosis events and poor quality clinical course. Although the risk of VTE in nonhospitalized COVID‐19 patients is not known yet, there are a large number of guidelines and studies on thromboprophylaxis administration for COVID‐19 cases. This study aims to take a detailed look at the effect of viral diseases on VTE, the epidemiology of VTE in viral diseases, and the diagnosis and treatment of VTE. [ABSTRACT FROM AUTHOR]

Throughout the ages the world has witnessed the outbreak of many infectious diseases. Emerging microbial diseases pose a serious threat to public health. Increasing resistance of microorganisms towards the existing drugs makes them ineffective. In fact, anti-microbial resistance is declared as one of the top public health threats by WHO. Hence, there is an urge for the discovery of novel antimicrobial drugs to combat with this challenge. Structural diversity and unique pharmacological effects make natural products a prime source of novel drugs. Staggeringly, in spite of its extensive biodiversity, a prominent portion of microorganism species remains unexplored for the identification of bioactives. Microorganisms are a predominant source of new chemical entities and there are remarkable number of antimicrobial drugs developed from it. In this review, we discuss the contributions of microorganism based natural products as effective antibacterial agents, studied during the period of 2010–2020. The review encompasses over 140 structures which are either natural products or semi-synthetic derivatives of microbial natural products. 65 of them are identified as newly discovered natural products. All the compounds discussed herein, have exhibited promising efficacy against various bacterial strains. [ABSTRACT FROM AUTHOR]

To measure the prevalence of viral infections, length of stay (LOS), and outcome in children admitted to the pediatric intensive care unit (PICU) during the period preceding the COVID-19 pandemic in a MERS-CoV endemic country. A retrospective chart review of children 0–14 years old admitted to PICU with a viral infection. Of 1736 patients, 164 patients (9.45%) had a positive viral infection. The annual prevalence trended downward over a three-year period, from 11.7% to 7.3%. The median PICU LOS was 11.6 days. Viral infections were responsible for 1904.4 (21.94%) PICU patient-days. Mechanical ventilation was used in 91.5% of patients, including noninvasive and invasive modes. Comorbidities were significantly associated with intubation (P-value = 0.025). Patients infected with multiple viruses had median pediatric index of mortality 2 (PIM 2) scores of 4, as compared to 1 for patients with single virus infections (p < 0.001), and a median PICU LOS of 12 days, compared to 4 in the single-virus group (p < 0.001). Overall, mortality associated with viral infections in PICU was 7 (4.3%). Patients with viral infections having multiple organ failure were significantly more likely to die in the PICU (p = 0.001). V iral infections are responsible for one-fifth of PICU patient-days, with a high demand for mechanical ventilation. Patients with multiple viral infections had longer LOS, and higher PIM 2 scores. The downward trend in the yearly rate of PICU admissions for viral infections between the end of the MERS-CoV outbreak and the start of the COVID-19 pandemic may suggest viral interference that warrants further investigations. [ABSTRACT FROM AUTHOR]

The ability of plant resistance inducers to provide protection against viral diseases is one of their main advantages over conventional pesticides. In the case of viral diseases that cannot be controlled directly with pesticides, insecticides are used to control the vectors of viruses. However, the effectiveness of such treatments is strictly dependent on the time of application. The plant response to the application of systemic acquired resistance (SAR) inducers, as a result of the stimulating action of these substances, does not depend on the time of application as it triggers the plant's natural defence mechanism. The best-recognised substance showing SAR inducer activity is acibenzolar-S-methyl ester (ASM, BTH). As its activity against different plant pathogens of crops has been well documented, the current research is concentrated on the search for novel substances of the type. The tested substance, N-methoxy-N-methylbenzo(1,2,3)thiadiazole-7-carboxamide (BTHWA), is an amide derivative of benzothiadiazole, showing plant resistance-inducing activity. This article presents the activity of BTHWA that has led to increased resistance of zucchini (Cucurbita pepo convar. giromontiina) towards viral infections. In addition, since the occurrence of the fungal pathogen, powdery mildew, was also observed during the two-year field experiments, the activity of BTHWA related to the reduction of infection with this fungus was also investigated. The substance was applied in two different variants either four or eight times, over the whole vegetation season. Surprisingly, the variant of four applications performed at the beginning of the vegetation season proved more effective in protection against viruses and fungus. A possible explanation may be the occurrence of the growth–immunity trade-off phenomenon that is known in the literature. Disturbance in plant metabolism resulting from eight applications may lead to lower yields of plants treated with SAR inducers. Perhaps such overstimulation of the plants we treated eight times may not have brought the optimum increase in plant resistance. [ABSTRACT FROM AUTHOR]

Objective: The COVID-19 pandemic, which was caused by SARS-CoV-2, has had a significant effect on global public health, economies, and societies worldwide. Serum antibody testing is a critical method for the diagnosis of COVID-19 and can complement RT-PCR in the diagnosis of COVID-19 patients; however, the performance of rapid antibody assays in the clinical setting has not been established. Methods: Rapid antibody assays were evaluated by investigating 296 COVID-19-positive individuals and 542 negative individuals confirmed by clinical diagnosis. The clinical diagnostic results were used as controls to evaluate the sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), kappa, and 95% confidence interval (CI) of the rapid tests. Result: IgM-positivity had a sensitivity of 86.1% and specificity of 99.1%. IgGpositivity had a sensitivity of 86.5% and specificity of 98.7%. The sensitivity of combined IgM- and IgG-positivity in clinically confirmed patients was 73.1% in the early stage (1-7 days after symptom onset) and reached 99% 15 days after symptom onset. The concordance between rapid antibody-positive tests and clinical diagnosis-positivity had a kappa value of 0.93. In addition, the falsepositive rate of IgM and IgG combined nucleic acid detection was 30% in the early stage. Conclusion: The combined use of IgM and IgG could serve as a more suitable alternative detection method for patients with COVID-19. The rapid antibody test can be considered as an excellent supplementary approach for detecting SARS-CoV-2 in clinical application. [ABSTRACT FROM AUTHOR]

Background: Little is known about Jordanian university students’ knowledge of viral disease. Aims: This study aimed to evaluate their knowledge of the nature of viruses and viral diseases among university students in Jordan. Methods: A cross-sectional study was conducted from May–September 2020, focused on students attending eleven colleges from three universities in Jordan. Students were asked to answer an online survey comprising 48 closed-ended questions measuring knowledge of viruses and viral diseases. Results: One thousand three hundred nine students responded to the questionnaire. Only 36.7% of the respondents said that they had studied viruses and viral diseases in university lectures. The findings revealed that 20.63% of the respondents thought that a virus is a living organism and 39.9% of the students correctly answered that viruses cannot reproduce on nonliving surfaces. On the other hand, more than one third of the participants thought that viruses are not sensitive to antibiotic treatment. Only 9.7% of the respondents could name the scientist who discovered the virus. Regarding viral diseases, 36.1% of the participants were aware that human papilloma virus causes cancer, while only 7.7% were aware that any of the viral hepatitis, herpes or human papilloma viruses can develop into cancer. Almost half of the students answered correctly that SARS-cov2 infects both humans and some animal species and that the HIV virus can be transmitted through sexual intercourse. Additionally, 54.7% of the students believe that vaccination is the best method of protecting humans from viral diseases. Lastly, 42.1% of the respondents were aware that viruses can be used in several scientific fields including genetics, immunology, molecular biology, and nanotechnology. Conclusion: These university students in Jordan have a poor knowledge of viruses and viral diseases and have had limited exposure to virology education. It is strongly suggested that their knowledge of viral diseases is improved at all levels of education through increasing the number of virology courses and lectures in educational institutions, especially health colleges. [ABSTRACT FROM AUTHOR]

Pregnancy is a period of elevated risk for viral disease severity, resulting in serious health consequences for both the mother and the fetus; yet antiviral drugs lack comprehensive safety and efficacy data for use among pregnant women. In fact, pregnant women are systematically excluded from therapeutic clinical trials to prevent potential fetal harm. Current FDA-recommended reproductive toxicity assessments are studied using small animals which often do not accurately predict the human toxicological profiles of drug candidates. Here, we review the potential of human maternal-fetal interface cellular models in reproductive toxicity assessment of antiviral drugs. We specifically focus on the 2- and 3-dimensional maternal placental models of different gestational stages and those of fetal embryogenesis and organ development. Screening of drug candidates in physiologically relevant human maternal-fetal cellular models will be beneficial to prioritize selection of safe antiviral therapeutics for clinical trials in pregnant women. [ABSTRACT FROM AUTHOR]

Scientific knowledge has a well-established cycle of generating hypotheses, testing them in experiments with proper discussion, and submitting it to the scientific community analysis through publications. It takes time to establish sample size for biomedical studies, especially concerning the effect of medicines and vaccines. The World Health Organization’s protocol estimates that more than 19 months of experiments are necessary to approve a vaccine, for example. As the world has witnessed, a pandemic with immediate impact on human lives urges scientific methods to speed up finding solutions. Here it was assessedthe speed and volume of information generated by the Academia to tackle the COVID-19 compared to the Swine Flu pandemic. Were considered papers published in journals indexed in PubMed, the most comprehensive biomedical scientific database available online. The number of publications about COVID-19 was 11 times higher than the number of publications about Swine Flu in a one-year timeframe. Though the expectation were findingmore international collaborations and studies focusing on vaccines for COVID-19, papers were mostly concentrated in China and studying symptoms, managing the pandemic, reviewing knowledge, or establishing clinical trials. For sure, science is working faster every day for solutions in biomedical critical situations. However, the fast volume of information might blurry decisions on public health management. This paper’s results show it is mandatory before using papers to take actions, waiting for the scientific community to first progress on its scientific knowledge cycle and mature discussions on the generated knowledge.

In latest years, researchers have been concerned about the resistance, recurrence, and dormancy of viruses that occurs in the prevention and treatment of viruses. Regarding the current pandemic conditions reported not only to the SARSCoV-2 virus (2019), but also to the SARS-CoV-1 virus (Severe Acute Respiratory Syndrome Coronavirus from 2013) and MERS-CoV (Middle East Respiratory Syndrome Coronavirus from 2018), it is mentioned that there are scientific publications that demonstrate the benefits of treatments with natural products. Some natural products rich in phytonutrients can be used as an alternative to the prevention and treatment of viral diseases along with some treatments with pharmaceuticals that are very good, but are more aggressive towards the body. Some of the natural products – to be more effective – are used in processed form – as essential oils, as products obtained by distillation under specific conditions, as tinctures or as aqueous or alcoholic extracts – these being tested by in vivo and in vitro experiments. Asia is one of the continent with a tradition in natural herbal treatments, and the origin of these pandemic viruses has further driven researchers to seek and develop new techniques and methods to obtain natural products – concentrated enough to be effective but not no harm to patients. Also, the World Health Organization promotes innovations regarding new potential treatments for new viral diseases and also welcomes traditional, alternative, and complementary medicine – which can have an important role in healthcare. And because some of the viral diseases are found also in animals and humans – the researchers have a very complicated job to find therapies that not only treat and prevent but also are efficient and safe!

Abstract Venous thromboembolism (VTE) is known to be a common respiratory and/or cardiovascular complication in hospitalized patients with viral infections. Numerous studies have proven human immunodeficiency virus infection to be a prothrombotic condition. An elevated VTE risk has been observed in critically ill H1N1 influenza patients. VTE risk is remarkably higher in patients infected with the Hepatitis C virus in contrast to uninfected subjects. The elevation of D‐dimer levels supported the association between Chikungunya and the Zika virus and the rise of clinical VTE risk. Varicella‐zoster virus is a risk factor for both cellulitis and the consequent invasive bacterial disease which may take part in thrombotic initiation. Eventually, hospitalized patients infected with the coronavirus disease of 2019 (COVID‐19), the cause of the ongoing worldwide pandemic, could mainly suffer from an anomalous risk of coagulation activation with enhanced venous thrombosis events and poor quality clinical course. Although the risk of VTE in nonhospitalized COVID‐19 patients is not known yet, there are a large number of guidelines and studies on thromboprophylaxis administration for COVID‐19 cases. This study aims to take a detailed look at the effect of viral diseases on VTE, the epidemiology of VTE in viral diseases, and the diagnosis and treatment of VTE.

Enzootic bovine leukosis (EBL) is a chronic viral disease of wide distribution in cattle herds and may take several years for the first manifestation of clinical signs. Most animals do not present clinical signs. However, the economic losses are underestimated due to this disease. Thus, this work aimed to detect and characterize BLV in dairy cattle in the Maranhão state, northeastern Brazil. Blood samples were collected from 176 animals from 8 municipalities in the southeastern state of Maranhão. Bovine blood samples were subjected to DNA extraction and molecular diagnosis using nested PCR assays for BLV, targeting gp51 gene. Positive samples were then sequenced and then subjected to phylogenetic inferences. BLV DNA was detected in 16 cattle (16/176, 9.09%) in 4 municipalities. Phylogenetic analyzes showed that the sequence obtained clustered in a clade containing BLV sequences classified as genotype 6, with a high degree of support. Our data shows BLV occurrence in the Northeast of Brazil and the identification of genotype 6 in this region. These findings contribute to the molecular epidemiology of this agent in Brazil.

For the last 3 decades the shrimp industries has been plagued by many destructive diseases, which have resulted in severe production and economic losses to many aquaculture countries. These include viral, bacterial and parasitic diseases. Recently, another emerging viral disease is threatening the shrimp culture industry in Asia. The virus originally called Cherax quadricarinatus iridovirus (CQIV) or Shrimp hemocyte iridescent virus (SHIV) and now classified within the proposed genus Decapodiridovirus and formally named as Decapod iridescent virus 1 (DIV1) by International Committee on Taxonomy of Viruses (ICTV). The virus was first detected as early as 2014 from Cherax quadricarinatus samples in Fujian Province and farmed white leg shrimp Penaeus vannamei samples from Zhejiang Province. This review article encompasses the significance of the DIV1 and their implications for the future of the global aquaculture. [ABSTRACT FROM AUTHOR]

This study aimed to survey the domestic dog populations and the prevalence of canine distemper virus(CDV),parvovirus(PV),rotavirus(RV),and rabies virus(RABV)in the potential risk area of the Wawu Mountain and Daxiangling Giant Panda Nature Reserve,Sichuan Province.In addition,an immune quarantine zone was established for various viral diseases,such as CDV,in the potential risk area,which can reduce the threat of viral infection and further protect the population of wild giant pandas(Ailuropoda melanoleuca).The total number,feeding method,body size,and sex of dogs in the potential risk area of Giant Panda Nature Reserve were recorded by house-to-house visits,questionnaire surveys,body weight and body length measurements; PCR technology and serum neutralizing antibody tests were used for the epidemiological investigation of CDV,PV,RV,and RABV on the subjects.The immunization of dogs for CDV,adenovirus type 2,parainfluenza and PV were administered via aquadruple live vaccine to establish the immune quarantine zone.The results showed that there were a total of 178 dogs in the potential risk area of Wawu Mountain and Daxiangling Giant Panda Nature Reserve,of which 51.7%(92/178)were free-ranging,and the majority of dogs(86.0%,153/178)were small and medium in size.The RV carried by dogs was giant panda RV(GPRV),with a positive rate of 76.9%(60/78).CDV and PV were not detected in the dogs.A total of 12.7%(7/55)of dogs were positive for the serum antibody of RABV.The immunization rate of vaccine was 88.8%(158/178).This study provides epidemiological data for the prevention and early warning of various viral diseases such as CDV in potential risk areas of the Giant Panda Nature Reserve,and provides scientific advice for the management of dogs around the Giant Panda Nature Reserve. [ABSTRACT FROM AUTHOR]

Bovine polyomavirus-1 (BoPyV-1, Epsilonpolyomavirus bovis) is widespread in cattle and has been detected in commercialized beef at supermarkets in the USA and Germany. BoPyV-1 has been questioned as a probable zoonotic agent with documented increase in seropositivity in people exposed to cattle. However, to date, BoPyV-1 has not been causally associated with pathology or disease in any animal species, including humans. Here we describe and illustrate pathological findings in an aborted bovine fetus naturally infected with BoPyV-1, providing evidence of its pathogenicity and probable abortigenic potential. Our results indicate that: (i) BoPyV-1 can cause severe kidney lesions in cattle, including tubulointerstitial nephritis with cytopathic changes and necrosis in tubular epithelial cells, tubular and interstitial inflammation, and interstitial fibroplasia; (ii) lesions are at least partly attributable to active viral replication in renal tubular epithelial cells, which have abundant intranuclear viral inclusions; (iii) BoPyV-1 large T (LT) antigen, resulting from early viral gene expression, can be detected in infected renal tubular epithelial cells using a monoclonal antibody raised against Simian Virus-40 polyomavirus LT antigen; and (iv) there is productive BoPyV-1 replication and virion assembly in the nuclei of renal tubular epithelial cells, as demonstrated by the ultrastructural observation of abundant arrays of viral particles with typical polyomavirus morphology. Altogether, these lesions resemble the "cytopathic-inflammatory pathology pattern" proposed in the pathogenesis of Human polyomavirus-1-associated nephropathy in immunocompromised people and kidney allograft recipients. Additionally, we sequenced the complete genome of the BoPyV-1 infecting the fetus, which represents the first whole genome of a BoPyV-1 from the Southern Hemisphere. Lastly, the BoPyV-1 strain infecting this fetus was isolated, causing a cytopathic effect in Madin–Darby bovine kidney cells. We conclude that BoPyV-1 is pathogenic to the bovine fetus under natural circumstances. Further insights into the epidemiology, biology, clinical relevance, and zoonotic potential of BoPyV-1 are needed. [ABSTRACT FROM AUTHOR]

Viruses of veterinary significance are known to survive for extended periods in plant‐based feed ingredients imported into North America. To reduce the likelihood of virus introduction, high‐risk ingredients, such as oil seed meals, are stored in designated facilities for extended periods under controlled environmental conditions to minimize viral infectivity prior to use in diets. While 30 days has become a standard storage period, the required ambient temperature to inactivate viruses during this time is not known. To address the question, 1‐metric tonne totes of conventional soybean meal were inoculated with PRRSV 144 lineage 1C variant and SVA prior to storage for 30 days at 23.9°C, 15.5°C or 10°C, and feeding to pigs. Virus infectivity was evaluated through detection of viral RNA in oral fluid samples, along with clinical signs. Results indicated that inactivation of both viruses occurred in soy stored at 23.9°C. In contrast, SVA infectivity was observed in soy stored at both 15.5°C and 10°C, while PRRSV 144 L1C variant infectivity was only observed in soy stored at 10°C. These results suggest that a storage period of 30 days and a temperature of 23.9°C may assist in the reduction of the risk of virus contaminated plant‐based feed ingredients, such as soybean meal. [ABSTRACT FROM AUTHOR]

The Indian poultry market is estimated to have an annual growth rate of 8.1% as of today. However, infectious diseases in poultry pose an important constraint in the growth and development of this sector in our region. Among infectious diseases, viral diseases of poultry pose a serious threat to the poultry industry from an economic point of view. Several viral disease outbreaks have been reported by various researchers from different parts of the country. Among the common viral diseases of poultry, incidences of Newcastle disease, Avian Influenza, Fowl Pox, Infectious Bursal Disease, Marek’s disease, Infectious Bronchitis, Infectious Laryngotracheitis and Inclusion Body Hepatitis are significant in Assam as well as other parts of India. Thorough epidemiological studies followed by the identification of different serotypes, pathotypes, strains, etc. by genotyping and molecular characterization of viral disease pathogens may lead to ways to control and eradicate the diseases. Importance should be given to maintaining basic preventive measures like biosecurity, farm hygiene, and proper vaccination. In a developing country like India, disease outbreaks can impact the country’s economy. In this study, a brief view of the common viral disease of poultry and its diagnosis and control strategies in Assam, India is depicted. However, this review well indicates a plethora of avian diseases that have occurred over the years causing a severe impact on poultry farming as a whole. [ABSTRACT FROM AUTHOR]

One of the significant reserves for further increasing the yield of berry crops is to protect them from pests and diseases. Among the latter, viral ones are especially dangerous. Therefore, methods of virus diagnostics and especially electron microscopy are of great importance, which makes it possible to see viral particles, determine their shape, size, localization in tissues, and identify anomalies in affected plant cells. Objective. To conduct a comparative study of healthy and diseased blackcurrant leaves in order to determine the degree of influence of the two viruses on the anatomical structure of organelles and inclusions, which can be used in the diagnosis and identification of viruses affecting plants. Methods. The material was blackcurrant plants with symptoms of reversion and green speckles, which are detected visually when examining the plantings of this crop. The morphology of viral particles, the anatomical structure of organelles, and inclusions were studied using the method of electron microscopy of ultrathin sections. Detected ultrastructural changes in cells can be used as diagnostic signs when identifying viruses. Also, viruses were identified by external signs and biological testing. Results. The study of ultrathin sections of leaf tissue and abnormal petals of the blackcurrant flower with symptoms of reversion revealed a bacillus-visible virus (Blackcurrant reversion virus) from the Rabdoviridae family, which is easy to identify due to its large size and appearance, in which it differs from similar features in ordinary cellular components. Typical locations of virus particles are the cytoplasm, nucleus, and perinuclear zone. The size of viral particles on ultrathin sections was 271 ± 7.19 nm long and 78 ± 2.31 nm in diameter. According to electron microscopic methods of studying artificially infected plants of Chenopodium quinoa, an inoculum of affected blackcurrant leaves, virions of Cucumis virus 1 Smith were observed in cells, which were freely located in the cytoplasm of the cell interspersed with ribosomes. Individual areas of the cytoplasm with a high virus concentration were also found in the affected parenchymal cells. Zones surrounded by a double membrane differ in the size and degree of virus saturation. When studying the pathogen morphology in the native preparations, the viral particles had a spherical shape with small, very colored central areas. Measurements of the virus particles showed that they had an average size of 29.6 ± 0.59 nm. During the study of the ultrastructure of blackcurrant plant cells affected by Cucumis virus 1. Smith, myelin-like bodies were found not only in the cytoplasm of affected cells but also in the extra-plasma space. Analysis of the morphology of chloroplasts of blackcurrant plants affected by green speckles and reversion shows that chloroplasts with outgrowths and cup-shaped formations are present in many cells. Under various viral infections, there is a wide variety of mitochondria's shapes: they are elongated, cup- or club-like, etc. At the same time, their internal structure changes as well. We found that at the stage of the neurotization of a Nicotiana tabacum leaf infected with Cucumis virus 1. Smith, the peroxisome matrix is intensively filled with crystalline inclusions that have an electron-dense surface or are a system of rods with different configurations in the form of rectangles and trapezoids. They completely fill the entire matrix. During the development of viral pathology in the cells of diseased plants, destructive processes also cover the nucleus. As a rule, it takes on a lobed or radially elongated shape. Among the viruses we studied, the Blackcurrant reverse virus causes this trait the most. This may be due to the fact that this virus, accumulated in large quantities, exerts mechanical pressure on the nuclei and thereby accelerates the process of their deformation. A peculiar sign of changes in the nucleus ultrastructure is the content of viral particles. Of the viruses we studied, blackcurrant reversal virus particles were the most common in the nucleus nucleoplasm and perinuclear zone. Conclusions. The intracellular development of viruses and their use of energy systems and components of plant cells for their reproduction lead to significant morphological and structural changes in the latter. In particular, electron microscopic studies of ultrathin tissue sections of diseased blackcurrant plants in comparison with healthy ones revealed the forms of the nucleus, mitochondria, and plastids modified under the influence of viral infection, namely Blackcurrant reverse and Cucumber mosaic viruses. Chloroplasts noticeably swelled without the existing content of starch grains, and clumping or the absence of gran thylakoids was observed. Plastids with a highly reduced membrane system were found. The results of studies have shown that the species affiliation of the virus does not cause specific changes in the morphology and structure of mitochondria. Their structural transformations under the influence of the viruses under study were the same: changes in shape and swelling, expansion of crists and a decrease in their number, a decrease in the electron density of their matrix, and so on. It was found that in the nuclei of cells infected with the Blackcurrant reverse virus, chromatin forms small, interconnected granular lumps located in different zones of the matrix. When studying ultrathin sections of Nicotiana tabacum leaf infected with Cucumis virus 1. Smith, rather specific crystal inclusions that fill the entire matrix were detected by the peroxisome. If external anomalies are detected in the form of mosaics, spots that can be caused by many pathogens in the absence of mechanical transmission of the pathogen, it is advisable to use the method of electron microscopy of ultrathin sections. [ABSTRACT FROM AUTHOR]

PANoptosis is a distinct inflammatory cell death mechanism that involves interactions between pyroptosis, apoptosis, and necroptosis. It can be regulated by diverse PANoptosome complexes built by integrating components from various cell death modalities. There is a rising interest in PANoptosis' process and functions. Viral infection is an important trigger of PANoptosis. Viruses invade host cells through their unique mechanisms and utilize host cell resources for replication and proliferation. In this process, viruses interfere with the normal physiological functions of host cells, including cell death mechanisms. A variety of viruses, such as influenza A virus (IAV), herpes simplex virus 1 (HSV1) and coronaviruses, have been found to induce PANoptosis in host cells. Given the importance of PANoptosis across the disease spectrum, this review briefly describes the relationships between pyroptosis, apoptosis, and necroptosis, highlights the key molecules in PANoptosome formation and activation, and outlines the multifaceted roles of PANoptosis in viral diseases, including potential therapeutic targets. We also talk about key principles and significant concerns for future PANoptosis research. Improved understanding of PANoptosis and its mechanisms is critical for discovering new treatment targets and methods., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)

Dynamics of the number and species composition of aphids were studied on seed potatoes using yellow water traps in the southern part of the Arkhangelsk region in 2018-2019. 12 species of winged aphids (165 insects) were identified in 2018, and 16 species (115 insects) - in 2019. The following types dominated: black bean aphid A. fabae Scop., bird cherry-oat aphid R. padi L., blackcurrant-sowthistle aphid H. lactucae L., glasshouse-potato aphid A. solani Kalt. The share of aphids as direct and indirect vectors of viral diseases was 91 % of the total number in 2018, and 79.1 % in 2019. The pest population of potato plants depended directly on weather conditions of the growing season. The sum of the average daily temperatures during the period of catching aphids was 1273.3 C in 2018, and 983.3 C - in 2019. During the experimental period, 131.6 mm of precipitation fell in 2018, and 280.4 mm - in 2019. Due to the constant presence of aphids on seed potato crops, annual monitoring of pest population and, in case of necessity, protective measures are required.

The dairy and meat industry has rapidly developed in the last decade in Turkey and is playing a key role in supplying animal proteins for human consumption. Viral pathogens continue to threaten the dairy and meat industry leading to serious economic losses worldwide, including Turkey. The Turkish cattle industry has been vulnerable to the spread of viral diseases within the country in the continent. Combating animal diseases is crucial for the economy of Turkey. A good cattle health management policy may reduce the direct losses associated with viral diseases and thereby lead to increase in export of animals and animal products. Countries that are unable to combat animal diseases remain excluded from international trade. Control and eradication of cattle diseases require the availability of effective and practical interventions including vaccination and biosecurity measures. This review summarises the currently available information about viral diseases in cattle in Turkey and emphasizes the need for disease monitoring and research, along with implementation of disease control measures to mitigate economic losses to farmers and the country. The information presented here can be of great value in the research, prevention, and control of cattle diseases. [ABSTRACT FROM AUTHOR]

Vegetatively propagated crops are particularly prone to disease dissemination through their seed systems. Strict phytosanitary measures are important to limit the impact of diseases as illustrated by the potato seed system in Europe. Cassava brown streak disease (CBSD) is a devastating disease caused by two viral species collectively named cassava brown streak viruses (CBSVs). CBSD can cause substantial root yield losses of up to 100% in the worst affected areas and is easily transmitted through stem cuttings. In Eastern and Central Africa, the epidemiology of CBSVs in the local socio-economical context of production remains poorly known while a better understanding would be an asset to properly manage the disease. This lack of information explains partially the limited efficiency of current regulatory schemes in increasing the availability of quality seed to smallholders and mitigating the spread of pests and diseases. This study surveyed the epidemiology of CBSVs in Uvira territory, Eastern D.R. Congo, and its drivers using a multivariate approach combining farmer's interview, field observation, sampling and molecular detection of CBSVs. Investigation on the epidemiology of CBSD revealed that three clusters in the study area could be identified using five most significant factors: (i) symptoms incidence, (ii) number of whiteflies, (iii) types of foliar symptoms, (iv) cutting's pathways and (v) plant age. Among the three clusters identified, one proved to be potentially interesting for seed multiplication activities since the disease pressure was the lowest. Through risk assessment, we also identified several key socio-economic determinants on disease epidemy: (i) factors related to farmer's knowledge and awareness (knowledge of cassava pests and diseases, knowledge of management practices, support from extension services and management strategies applied), (ii) factors related to the geographical location of farmer's fields (proximity to borders, proximity to town, distance to acquire cuttings), as well as (iii) the pathways used to acquire cuttings. [ABSTRACT FROM AUTHOR]

Viral diseases are very common in both man and animals and these are effecting both creatures. Most viral Infections are severe enough to kill birds or animals e.g ND and IB in birds become the cause of the highest mortality ratio. In this study, we will give a possible solution to combat viral infection and also give the Policy to save poultry birds from viral infections. This study gives an idea to save birds from viral infection. This study completely describes the effect of vaccine. This review shows, if we do the right management we can minimize the risk of viral infections the focus of this study is on four things. 1) Describe the viral Diseases. 2) Policy to control viral Disease. 3)Bring light toward importance of vaccination 4) Method to reduce the spread out of disease This study also describes the diagnose methods of the disease, and if a person doesn’t know much about the viral disease of poultry can also know about the disease by reading that study. [ABSTRACT FROM AUTHOR]

Abstract The objective of this work was to evaluate the infection caused by influenza A virus (IAV) subtypes and its incidence in pig nurseries in the state of Santa Catarina, Brazil. A total of 423 nursery pigs were sampled in 11 farms, and IAV circulation, viral RNA, and antibodies were identified. Reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) was used to detect viral RNA in nasal swab samples (70.2%) and to subtype 33 viruses, of which 18 (54.5%) from six nurseries were the H3N2 virus, 6 (18.1%) from two nurseries were H1N1pdm, and 9 (27.2%) from three nurseries could not be subtyped. The enzyme-linked immunosorbent assay (ELISA) detected the presence of IAV antibodies (68%), which was confirmed by the hemagglutination inhibition test, revealing a higher prevalence of antibodies for the H3N2 virus (38.0%), followed by H1N1pdm (23.8%) and H1N2 (3.23%). The obtained data showed that 10.3% of the swine reacted to at least two viral antigens. There is a high prevalence of influenza A virus infection in all 11 piglets nurseries sampled by viral RNA and antibody detection. H3N2 and H1N1pdm, in this order, are the most detected viral subtypes in the 11 sampled nurseries.

Many pathogenic viruses infect camels, generally regarded as especially hardy livestock because of their ability to thrive in harsh and arid conditions. Transmission of these viruses has been facilitated by the commercialization of camel milk and meat and their byproducts, and vaccines are needed to prevent viruses from spreading. There is a paucity of information on the effectiveness of viral immunizations in camels, even though numerous studies have looked into the topic. More research is needed to create effective vaccines and treatments for camels. Because Camels are carriers of coronavirus, capable of producing a powerful immune response to recurrent coronavirus infections. As a result, camels may be a suitable model for viral vaccine trials since vaccines are simple to create and can prevent viral infection transfer from animals to humans. In this review, we present available data on the diagnostic, therapeutic, and preventative strategies for the following viral diseases in camels, most of which result in significant economic loss: camelpox, Rift Valley fever, peste des petits ruminants, bovine viral diarrhea, bluetongue, rotavirus, Middle East respiratory syndrome, and COVID-19. Although suitable vaccines have been developed for controlling viral infections and perhaps interrupting the transmission of the virus from the affected animals to blood-feeding vectors, there is a paucity of information on the effectiveness of viral immunizations in camels and more research is needed. Recent therapeutic trials that include specific antivirals or supportive care have helped manage viral infections.

Vegetatively propagated crops are particularly prone to disease dissemination through their seed systems. Strict phytosanitary measures are important to limit the impact of diseases as illustrated by the potato seed system in Europe. Cassava brown streak disease (CBSD) is a devastating disease caused by two viral species collectively named cassava brown streak viruses (CBSVs). CBSD can cause substantial root yield losses of up to 100% in the worst affected areas and is easily transmitted through stem cuttings. In Eastern and Central Africa, the epidemiology of CBSVs in the local socio-economical context of production remains poorly known while a better understanding would be an asset to properly manage the disease. This lack of information explains partially the limited efficiency of current regulatory schemes in increasing the availability of quality seed to smallholders and mitigating the spread of pests and diseases. This study surveyed the epidemiology of CBSVs in Uvira territory, Eastern D.R. Congo, and its drivers using a multivariate approach combining farmer’s interview, field observation, sampling and molecular detection of CBSVs. Investigation on the epidemiology of CBSD revealed that three clusters in the study area could be identified using five most significant factors: (i) symptoms incidence, (ii) number of whiteflies, (iii) types of foliar symptoms, (iv) cutting’s pathways and (v) plant age. Among the three clusters identified, one proved to be potentially interesting for seed multiplication activities since the disease pressure was the lowest. Through risk assessment, we also identified several key socio-economic determinants on disease epidemy: (i) factors related to farmer’s knowledge and awareness (knowledge of cassava pests and diseases, knowledge of management practices, support from extension services and management strategies applied), (ii) factors related to the geographical location of farmer’s fields (proximity to borders, proximity to town, distance to acquire cuttings), as well as (iii) the pathways used to acquire cuttings.

Since the beginning of time, microorganisms have been in existence. With time, new pathogens have emerged as a result of complex interplay of anthropogenic and natural factors like, human migration, shifts in weather pattern, genetic shuffling of the organisms themselves and more which have been discussed in detail. This review article focuses solely on emerging and re-emerging viruses: Chikungunya, Coronavirus, Dengue, Ebola, Hepatitis C, Herpes Simplex Virus (HSV), Human Immunodeficiency Virus (HIV), Human Papilloma Virus (HPV), Influenza and Zika; and the latest progresses made in finding effective anti-viral drugs via drug repurposing as we know this approach outplays de novo production significantly with respect to time and money. In a time where new diseases are being reported once every year, drug repurposing will certainly come in handy in developing anti-viral therapeutics promptly. Additionally, the study elaborates on the mechanisms of actions of these drugs as well as the targets whilst including recent and well-known incidences of deadly, viral outbreaks. [ J Adv Biotechnol Exp Ther 2021; 4(1.000): 74-94]

Sudden non-cardiac death (SNCD) is a clinical entity comprising deaths lacking previous clinically significant symptoms, and in which the mechanisms of death do not involve the heart. Infection is a major cause of SNCD, particularly in children, and viruses are frequently involved in the disease process. Nevertheless, SNCD of viral infectious causes remains poorly characterized. Thus, a systematic review of the literature describing the association between viral infection and the development of SNCD was performed. PRISMA statement guidelines were followed in this systematic review. A literature search was conducted across MEDLINE, Scopus and Web of Science databases. Studies considered eligible were autopsy series or cohort studies of sudden death cases, in which evidence of viral disease as a cause of death was demonstrated, along with identification of causative agents. Twelve studies published between 1996 and 2020 were included in this review. Selected studies were categorized into three groups according to the study population: infants and young children (up to four years of age); presumed sudden infant death syndrome patients; and older individuals (five years of age and older). SNCD with viral implication represents a minority of sudden death cases in all age groups, with infants and young children having a higher prevalence across studies. Respiratory infection was the main cause of viral SNCD, with influenza virus and respiratory syncytial virus being the most commonly identified agents in older individuals, and infants and young children respectively. Disseminated infection, gastrointestinal infection, and meningitis were other identified causes of SNCD in children. No studies have directly assessed the frequency and causes of viral SNCD. Infants and young children show a considerable, but variable, prevalence of this clinical entity. Wider implementation of post-mortem virological molecular testing may help uncover previously unknown cases. More research into viral SNCD is needed, especially in the adult population. • Viral sudden non-cardiac death comprises a considerable minority of sudden deaths. • Respiratory infection and disseminated infection are the main causes of death. • Respiratory syncytial virus and Herpes simplex virus are key causative agents. • Molecular post-mortem virology may uncover previously unknown causes of death. • Viral sudden non-cardiac death is understudied, especially in the adult population. [ABSTRACT FROM AUTHOR]

[Display omitted] Advancements in nanotechnology were vastly applied in medicine and pharmacy, especially in the field of nano-delivery systems. It took a long time for these systems to ensure precise delivery of very delicate molecules, such as RNA, to cells at concentrations that yield remarkable efficiency, with success rates reaching 95.0% and 94.5%. These days, there are several advantages of using nanotechnological solutions in the prevention and treatment of cancer and viral infections. Its interventions improve treatment outcomes both due to increased effectiveness of the drug at target location and by reducing adverse reactions, thereby increasing patient adherence to the therapy. Based on the current knowledge an updated review was made, and perspective, opportunities and challenges in nanomedicine were discussed. The methods employed include comprehensive examination of existing literature and studies on nanoparticles and nano-delivery systems including both in vitro tests performed on cell cultures and in vivo assessments carried out on appropriate animal models, with a specific emphasis on their applications in oncology and virology. This brings together various aspects including both structure and formation as well as its association with characteristic behaviour in organisms, providing a novel perspective. Furthermore, the practical application of these systems in medicine and pharmacy with a focus on viral diseases and malignancies was explored. This review can serve as a valuable guide for fellow researchers, helping them navigate the abundance of findings in this field. The results indicate that applications of nanotechnological solutions for the delivery of medicinal products improving therapeutic outcomes will continue to expand. [ABSTRACT FROM AUTHOR]

Viral diseases have caused great economic losses to the aquaculture industry. However, there are currently no specific drugs to treat these diseases. Herein, we utilized Siniperca chuatsi as an experimental model, and successfully extracted two tissue factor pathway inhibitors (TFPIs) that were highly distributed in different tissues. We then designed four novel peptides based on the TFPIs, named TS20, TS25, TS16, and TS30. Among them, TS25 and TS30 showed good biosafety and high antiviral activity. Further studies showed that TS25 and TS30 exerted their antiviral functions by preventing viruses from invading Chinese perch brain (CPB) cells and disrupting Siniperca chuatsi rhabdovirus (SCRV)/ Siniperca chuatsi ranairidovirus (SCRIV) viral structures. Additionally, compared with the control group, TS25 and TS30 could significantly reduce the mortality of Siniperca chuatsi , the relative protection rates of TS25 against SCRV and SCRIV were 71.25 % and 53.85 % respectively, and the relative protection rate of TS30 against SCRIV was 69.23 %, indicating that they also had significant antiviral activity in vivo. This study provided an approach for designing peptides with biosafety and antiviral activity based on host proteins, which had potential applications in the prevention and treatment of viral diseases. [Display omitted] • Novel TS25 and TS30 peptides designed based on TFPIs from Siniperca chuatsi. • TS25 and TS30 showed good biosafety and high antiviral activity. • TS25 and TS30 produced antiviral functions by preventing viruses from invading cells and disrupting viral structures. • TS25 and TS30 could significantly reduce the mortality of Siniperca chuatsi. [ABSTRACT FROM AUTHOR]

With an ever-increasing human population, food security remains a central issue for the coming years. The magnitude of the environmental impacts of food production has motivated the assessment of the environmental and health benefits of shifting diets, from meat to fish and seafood. One of the main concerns for the sustainable development of aquaculture is the emergence and spread of infectious animal diseases in a warming climate. We conducted a meta-analysis to investigate the influence of global warming on mortality due to viral infections in farmed aquatic animals. We found a positive trend between increasing temperature and increasing viral virulence, with an increase in water temperature of 1 °C resulting in an increase in mortality of 1.47–8.33% in OsHV-1 infected oysters, 2.55–6.98% in carps infected with CyHV-3 and 2.18–5.37% in fishes infected with NVVs. We suggest that global warming is going to pose a risk of viral disease outbreaks in aquaculture and could compromise global food security.

Biosensors are analytical tools that can be used as simple, real-time, and effective devices in clinical diagnosis, food analysis, and environmental monitoring. Nanoscale functional materials possess unique properties such as a large surface-to-volume ratio, making them useful for biomedical diagnostic purposes. Nanoengineering has resulted in the increased use of nanoscale functional materials in biosensors. Various types of nanostructures i.e., 0D, 1D, 2D, and 3D, have been intensively employed to enhance biosensor selectivity, limit of detection, sensitivity, and speed of response time to display results. In particular, carbon nanotubes and nanofibers have been extensively employed in electrochemical biosensors, which have become an interdisciplinary frontier between material science and viral disease detection. This review provides an overview of the current research activities in nanofiber-based electrochemical biosensors for diagnostic purposes. The clinical applications of these nanobiosensors are also highlighted, along with a discussion of the future directions for these materials in diagnostics. The aim of this review is to stimulate a broader interest in developing nanofiber-based electrochemical biosensors and improving their applications in disease diagnosis. In this review, we summarize some of the most recent advances achieved in point of care (PoC) electrochemical biosensor applications, focusing on new materials and modifiers enabling biorecognition that have led to improved sensitivity, specificity, stability, and response time.

In the beginning of the third year of the fight against COVID-19, the virus remains at least still one step ahead in the pandemic "war". The key reasons are evolving lineages and mutations, resulting in an increase of transmissibility and ability to evade immune system. However, from the immunologic point of view, the cytokine storm (CS) remains a poorly understood and difficult to combat culprit of the extended number of in-hospital admissions and deaths. It is not fully clear whether the cytokine release is a harmful result of suppression of the immune system or a positive reaction necessary to clear the virus. To develop methods of appropriate treatment and therefore decrease the mortality of the so-called COVID-19-CS, we need to look deeply inside its pathogenesis, which is the purpose of this review. [ABSTRACT FROM AUTHOR]

The rapid development of nanomedicine has created a high demand for silver, copper and copper oxide nanoparticles. Due to their high reactivity and potent antimicrobial activity, silver and copper-based nanomaterials have been playing an important role in the search for new alternatives for the treatment of several issues of concern, such as pathologies caused by bacteria and viruses. Viral diseases are a significant and constant threat to public health. The most recent example is the pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this context, the object of the present review is to highlight recent progress in the biomedical uses of these metal nanoparticles for the treatment and prevention of human viral infections. We discuss the antiviral activity of AgNPs and Cu-based NPs, including their actions against SARS-CoV-2. We also discuss the toxicity, biodistribution and excretion of AgNPs and CuNPs, along with their uses in medical devices or on inert surfaces to avoid viral dissemination by fomites. The challenges and limitations of the biomedical use of these nanoparticles are presented. [ABSTRACT FROM AUTHOR]

Five PCV2 strains identified in this study showed the highest nt identities (99.2-100%) to PCV2 strains retrieved from the GenBank database whilst strain ITA/2021/434 was more distantly related (88.8% nt identity) to strain 71b Vicenza 36 (GenBank accession nr KP231135) identified from a domestic pig in Italy in 2008. The complete PCV genome sequence was obtained only from 11 (5 PCV2 and 6 PCV3) Italian wild boar (Table S4). The prevalence of PCV3 in wild boar in our survey was nearly as high (71%) as in Sardinia Island (77.39% in free ranging pigs and 61.54% in wild boar) [[17]], but higher than the prevalence of PCV3 detected in the Campania region (49.32%) [[15]] and in other Mediterranean countries (e.g., 42.66% in Spain) [[11]]. [Extracted from the article]