Search

Objective To observe the neuroprotective effect of herbal medicine Gengnianchun formula (GNC) on primary cultured rat fetal hippocampal neurons injury induced by amyloid beta 25-35 fragment (Aβ25-35)through ERβ and PI3K/Akt signaling pathway. Methods Human neuroblastoma cell line (SH-SY5Y) were treated with Aβ25-35 to establish an in vitro cell model of Alzheimer's disease (AD) and were cultured with different concentration of GNC serum (GS) and Aβ25-35 to determine the appropriate treatment concentration and duration of these drugs by cell viability test (CCK-8) method. Primary cultured rat fetal hippocampal neurons were treated with Aβ25-35 to establish an in vitro cell model of AD and were divided into 5 groups,including control group,model group,GS group,normal saline serum(NS) group and PHTPP(a selective ERβ antagonist) group. Morphological observation,CCK-8 test and LDH release assay were used to evaluate the cell injury of primary cultured rat fetal hippocampal neurons. Hoest33258/PI double fluorescence staining,qRT-PCT and Western blot experiments were used to evaluate the cell apoptosis of primary cultured rat fetal hippocampal neurons. Results Compared with the control group,synaptic shrink,collapse and cell debris of primary cultured rat fetal hippocampal neurons were more obvious,cell viability decreased and LDH release increased in model group. Compared with the model group and NS group,synaptic shrink,collapse and cell debris of primary cultured rat fetal hippocampal neurons reduced,cell viability increased,LDH release decreased,number of PI positive primary cultured rat fetal hippocampal neurons were decreased,levels of bax/bcl-2 and cyt-c mRNA and the protein levels of bax/bcl-2,cleaved-caspase-3 and cyt-c were down-regulated and level of p-Akt was up-regulated in GS group. Compared with GS group,levels of bax/bcl-2 and cyt-c mRNA and the protein levels of bax/bcl-2,cleaved-caspase-3 and cyt-c were up-regulated and level of p-Akt was down-regulated in PHTPP group.Conclusions Neuroprotective effect of GNC on primary cultured rat fetal hippocampal neurons injury was mediated by or at least partly by ERβ and PI3K/Akt signaling pathway. [ABSTRACT FROM AUTHOR]

Copyright of Transactions of China Pulp & Paper is the property of China Pulp & Paper Magazines Publisher and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Transactions of Beijing Institute of Technology is the property of Beijing University of Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Medical Equipment Journal is the property of Chinese Medical Equipment Journal Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Mine Automation is the property of Industry & Mine Automation Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Engineering Mechanics / Gongcheng Lixue is the property of Engineering Mechanics Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

目的 探讨局部皮瓣联合耳前带蒂组织移植矫治耳垂发育不良的临床效果。方法 自 2008 年 1 月至 2020 年 1 月，华中科技大学同济医学院附属协和医院整形外科对 8 例先天性耳垂发育不良患者进行手术治疗，在耳垂下方设计切口， 形成一皮瓣，局部旋转缝合，以增加耳垂的皮肤；再于耳前形成一蒂在耳垂前的真皮脂肪瓣，将之插入耳垂前外侧与后内侧皮肤 之间分离形成的腔隙内，缝合固定，缝合供瓣区切口。结果 所有患者的切口均一期愈合，无皮瓣坏死发生。1 例患者出现切口 瘢痕增生，经外用药物涂抹等治疗后瘢痕软化。所有患者仅经 1 次手术治疗；获随访 6~18 个月，无脂肪液化、感染、硬结、 皮下包 块等并发症发生。手术后耳垂形状正常，较术前明显饱满，患者对耳垂外观满意度较高。结论 采用自体局部皮瓣联合带蒂真皮 脂肪组织转移修复，可明显改善耳垂外观，效果稳定持久。 [ABSTRACT FROM AUTHOR]

Copyright of Systems Engineering & Electronics is the property of Journal of Systems Engineering & Electronics Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Cellulose nanocrystallirie/Fes 0i nanospheres were iri-situ synthesized with cellulose nanocrystalline (CNC). The particle size？ crystallization？ magnetic properties and electrochemical properties of CNC/Fe.s0-i nanospheres were analyzed. The results show that CNC/F:> O-i nanospheres were uniform in size with a diameter of 1 0-30 nm. The magnetization of CNC/Fe.sO-i rianospheres was 34.9 emu/g. The specific capacitance of the CNC/Fe 0.•! was mainly the tantalum capacitor generated by Fe").卜 The CNC/Fe.sO-i nanospheres showed a specific capacitance of 30.14 F / g at 0.03 A / g in 1 mol / LNa S0; aqueous solution. The specific capacitance retention was about 78.76% at 0.04 A / g after 500 cycles. The diffusion of ions in the CNC / Fe") i was the Warburg mechanism. [ABSTRACT FROM AUTHOR]

Copyright of China Mechanical Engineering is the property of Editorial Board of China Mechanical Engineering and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of China University of Petroleum is the property of China University of Petroleum and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of South Architecture / Nanfang Jianzhu is the property of South Architecture Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Petrochemical Universities / Shiyou Huagong Gaodeng Xuexiao Xuebao is the property of Journal Editorial Department Of Liaoning Shihua University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Cotton Textile Technology is the property of Cotton Textile Technology Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Medical Equipment Journal is the property of Chinese Medical Equipment Journal Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Modern Laboratory Medicine is the property of Journal of Modern Laboratory Medicine Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of South Architecture / Nanfang Jianzhu is the property of South Architecture Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Mechanical Engineering is the property of Editorial Board of China Mechanical Engineering and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Acta Prataculturae Sinica is the property of Acta Prataculturae Sinica Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

[Objective] MEP pathway is one of the important ways to synthesize alkaloids and terpenoid precursors, and 1 -deoxy-D-xylulose-5-phosphate synthase (DXS) is the first pivotal enzyme in the MEP pathway. In order to understand the structure and function of Fritillaria DXS, molecular biology were used to analyze it, which established a foundation for the synthesis of Fritillaria alkaloids. [Method] The open reading frame (ORF) sequence of FcDXS gene was cloned by PCR and sequenced by bioinformatics to predict its potential function. The expressions of FcDXS gene in the wild (root, stem, leaf, bulb) and tissue culture inducer (callus) were measured by qRT-PCR. [Result] The 2151 bp ORF fragment of FcDXS gene was obtained and encoded a polypeptide of 716 amino acids with a similarity of more than 80 % to the plant DXS protein such as Amomum villosum published by NCBI. In the secondary and tertiary structure prediction of FcDXS protein, it was concluded that the FcDXS protein consists mainly of a-helix; The results of qRT-PCR showed that the expression of FcDXS gene in the wild was the highest, and the expression in hormone-induced callus was 5 times that in the wild bulb. [Conclusion] Bioinformatics analysis of FcDXS protein features and homology showed that the protein was conserved domain, combining with fluorescence quantitative analysis results proved that FcDXS may be a biologically functional and hormone-induced expression of the protein. This study established a theoretical foundation for the subsequent use of genetic engineering to improve the content of alkaloids of Fritillaria cirrhosa. [ABSTRACT FROM AUTHOR]

Copyright of Cotton Textile Technology is the property of Cotton Textile Technology Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

目的观察小儿妓翘清热颗粒联合重组人干扰素alb 治疗小儿急性上呼吸道感染( AURI) 的临床疗效。 方法选取2018 年1 月至2019 年3 月该院收治的AURI 患儿122 例作为研究对象， 按照治疗方案的不同将其分为对照组和治 疗组，每组各61 例。对照组仅子以重组人干扰素alb 注射液雾化吸入治疗， 冶疗组在对照组基础上加用小儿妓翘清热颖杠冶疗， 2 组均连续治疗1 周。比较2 组临床疗效， 以及治疗前后血清促炎因子、抑炎因子水平。结果治疗后， 治疗组血清肿瘤坏死因 子(TNF) a、白细胞介素4(IL 4) 、IL 6、IL 8、IL 10 水平均明显低于对照组， 而IL 2 水平则高于对照组， 差异均有统计学意义 (P<0. 05) 。治疗组临床疗效明显高于对照组， 差异有统计学意义(P<0. 05) 。结论小儿妓翘清热颖粒联合重组人干扰素alb 可有效改善AURI 患儿机体炎症状态， 疗效显著，值得临床推广。 [ABSTRACT FROM AUTHOR]

Copyright of Forest Research is the property of Forest Research Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Clinical Healthcare is the property of Chinese Journal of Clinical Healthcare and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Southern Agriculture is the property of Journal of Southern Agriculture and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The treatment of premenopausal patients with endometrial cancer has not yet been standardized, first of all, drug dosage, drug intake, treatment time and so on, there are no clear standards. Secondly, because of the high recurrence rate, is it unclear whether the patient needs to be removed after delivery. By reading a large number of literature and clinical prospective study, the main drug treatment is given priority to with low dose, treatment time is generally about 9-12 months. In addition to the traditional simple oral hormone therapy, in recent years, the treatment of LNG-IUS, the LNG-IUS and GnRHa combined therapy, hysteroscopic surgery and hormone combination therapy are gradually carried out. And through assisted reproductive technology, the success rate of pregnancy is increasing after treatment. Research shows that the treatment of premenopausal endometrial cancer is very promising at present, but a large number of clinical trials are needed to improve the treatment strategy. In this paper, by combining with clinical practice and the literature, we do a review about indication selection, pretreatment evaluation, treatment plan, curative effect evaluation, condition monitoring, and the problems that need to be faced after birth. [ABSTRACT FROM AUTHOR]

Copyright of Engineering Mechanics / Gongcheng Lixue is the property of Engineering Mechanics Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Modern Laboratory Medicine is the property of Journal of Modern Laboratory Medicine Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Society: Chinese Journal of Sociology / Shehui is the property of Society: Chinese Journal of Sociology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Sclerostin,a protein encoded by sclerosteosis( SOST) gene,antagonizes the Wnt protein via the low density lipoprotein receptor-related protein( LRP5/6) receptor and inhibits bone formation. It has been a new target for the treatment of osteoporosis.Both basic and clinical researches have indicated that sclerostin monoclonal antibody can treat osteoporosis by increasing bone mineral density,promoting bone formation and inhibiting bone resorption. However,limited data have evaluated the adverse effects of sclerostin monoclonal antibody and its influence on bone fracture, which deserve further studies. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effect of Artesunate (Art) on the expression of transferrin receptor (TtR)in K562/ADM cells. Methods: The drug-resistant K562/ADM cells were cultured with 1000 ng/mL doxorubicin for two weeks followed by Artesunate treatment with different concentrations (12.5 μg/mL, 25μg/mL and 50 μg/mL) or different time (12 h, 24 h, 36 h, and 48 h). The content of transferrin receptor in K562/ADM cells was determined by flow cytometry. The effect of Artesunate on the expression of transferrin receptor protein in K562/ADM cells was measured by Westem blot. Cell counting kit-8 (CCK-8) assay was used to evaluate inhibitory effect of Art combined with doxorubicin (ADM) in K562/ADM cells.The reversal index was defined as the IC50 of the experimental group divided by the IC50 of the control group in K562/ADM cells. Results: Art effectively decreased the content of transferrin receptor and the expression of transferrin receptor protein in K562/ADM cells in a dose-dependent manner. Moreover, Art also inhibited transferrin receptor protein expression in K562/ADM cells in a time-dependent manner.The different concentrations of Art(12.5 μg/mL, 25μg/mL and 50 μg/mL) could induce reversal of drug-resistance with the reversal index being 1.38, 2.12 and 2.95 times (P＜0.05). Art inhibited cell proliferation of K562/ADM cells, and the IC50 were19.7 μmol/L. Conclusions: Art effectively down-regulated the transferrin receptor content as well as transferrin receptor protein expression in K562/ADM cells, which resulted in reversal of drug resistance of K562/ADM cells. Art also inhibited K562/ADM cells proliferation, which has great value in clinical treatment of leukemia. [ABSTRACT FROM AUTHOR]

Copyright of Application Research of Computers / Jisuanji Yingyong Yanjiu is the property of Application Research of Computers Edition and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Application Research of Computers / Jisuanji Yingyong Yanjiu is the property of Application Research of Computers Edition and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of the Tianjin Polytechnic University / Tianjin Gongye Daxue Xuebao is the property of Journal of Tianjin Polytechnic University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Application Research of Computers / Jisuanji Yingyong Yanjiu is the property of Application Research of Computers Edition and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In order to study the mechanical behavior of reinforced concrete columns strengthened with high ductile concrete (HDC), eight reinforced concrete columns were designed and strengthened with HDC jacket and high performance composite mortar jacket. The failure modes, deformation capacity and energy dissipation capacity of the specimens were studied by low cyclic loading tests. The test results show that HDC jacket could provide good constraining at the core concrete. The failure mode is changed from brittle failure to ductile failure, and the bearing capacity, ductility and energy dissipation capacity of the strengthened columns are significantly improved after strengthening. The good synergic work performance between HDC jacket and concrete could take advantage of the high damage tolerance capacity of HDC, and the HDC jacket is much more effective than the mesh reinforced high performance composite mortar in behavior enhancement of the columns. Configuring mesh reinforcements in the HDC jacket could improve the constraining effect of the jacket on the inner concrete, and further improve the ductility and energy dissipation capacity of the strengthened columns, but contribute little to the bearing capacity of the strengthened columns. Using HDC jacket to strengthen concrete columns could reduce the damage degree of the vertical components and improve the seismic behavior of the components. [ABSTRACT FROM AUTHOR]

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)