151. 中药更年春方通过ERβ及PI3K/Akt信号通路对Aβ25-35致大鼠胎鼠海马神经元损伤的保护作用
- Author
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张阳, 高玲芸, and 王文君
- Abstract
Objective To observe the neuroprotective effect of herbal medicine Gengnianchun formula (GNC) on primary cultured rat fetal hippocampal neurons injury induced by amyloid beta 25-35 fragment (Aβ25-35)through ERβ and PI3K/Akt signaling pathway. Methods Human neuroblastoma cell line (SH-SY5Y) were treated with Aβ25-35 to establish an in vitro cell model of Alzheimer's disease (AD) and were cultured with different concentration of GNC serum (GS) and Aβ25-35 to determine the appropriate treatment concentration and duration of these drugs by cell viability test (CCK-8) method. Primary cultured rat fetal hippocampal neurons were treated with Aβ25-35 to establish an in vitro cell model of AD and were divided into 5 groups,including control group,model group,GS group,normal saline serum(NS) group and PHTPP(a selective ERβ antagonist) group. Morphological observation,CCK-8 test and LDH release assay were used to evaluate the cell injury of primary cultured rat fetal hippocampal neurons. Hoest33258/PI double fluorescence staining,qRT-PCT and Western blot experiments were used to evaluate the cell apoptosis of primary cultured rat fetal hippocampal neurons. Results Compared with the control group,synaptic shrink,collapse and cell debris of primary cultured rat fetal hippocampal neurons were more obvious,cell viability decreased and LDH release increased in model group. Compared with the model group and NS group,synaptic shrink,collapse and cell debris of primary cultured rat fetal hippocampal neurons reduced,cell viability increased,LDH release decreased,number of PI positive primary cultured rat fetal hippocampal neurons were decreased,levels of bax/bcl-2 and cyt-c mRNA and the protein levels of bax/bcl-2,cleaved-caspase-3 and cyt-c were down-regulated and level of p-Akt was up-regulated in GS group. Compared with GS group,levels of bax/bcl-2 and cyt-c mRNA and the protein levels of bax/bcl-2,cleaved-caspase-3 and cyt-c were up-regulated and level of p-Akt was down-regulated in PHTPP group.Conclusions Neuroprotective effect of GNC on primary cultured rat fetal hippocampal neurons injury was mediated by or at least partly by ERβ and PI3K/Akt signaling pathway. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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