Potential spatial conflicts arise as a result of different uses of land resources adjacent to each other. A tradeoff can be realized between economic development and ecological environment in recent years. Therefore, the spatial conflict of Land Use (SCLU) needs to be considered from the three dimensions of natural resources, space, as well as human rights and interests. There is also the continuous change of land use types in the spatial perspective. Therefore, it is a high demand to determine the location of potential SCLU from the perspective of the coordination between human activities and natural environment. In this study, an ecologically sensitive area was first chosen to clarify the connotation of SCLU from the perspective of ecological environment. Secondly, the conflict identification and measurement were constructed using the “risk effect” through “conflict risk index” and “ecological environment quality index”. It was also considered the changes in landscape patterns and ecological environment caused by land use. Finally, a case study was carried out in the Hinggan League of Inner Mongolia, China, from 2000 to 2020. An emphasis was then placed on the construction of ecological civilization as a national strategy for the sustainable and healthy development of the economy. The SCLU caused two consequences: the change of spatial pattern and negative effects. The former was the general consequence of space conflict, whereas, the latter presented different issues under various conditions. Specifically, the fragments of space were differentiated to determine the optimal resource allocation, according to the natural value. The regional spatial pattern alleviated the contradiction between natural sources and land use. As such, there was no spatial conflict after the optimization of territorial spatial structure. The potential SCLU was also considered that the change of spatial pattern brought a certain risk of conflict, in order to prevent the possible negative effects during the assessment. The results showed that: 1) The SCLU was the proper consequence of the further intensification in the contradiction of land use, indicating a negative impact on the society, economy, and ecology. In essence, the SCLU was the representation of the potential spatial conflict of interest between the different stakeholders in the process of land use. 2) The grassland was dominated the land development and utilization in the study area, indicating a significant increase in the intensity of land use from 2010 to 2020, compared with the previous decade. The area of spatial conflict also increased significantly from 2010 to 2020, particularly with the relatively higher growth rate of severe conflict. The ever-increasing conflict risk and index of eco-environmental quality demonstrated the significant disturbance in the land system stability from 2010 to 2020. 3) The main fragments of the SCLU were gradually shifted to the southeast of Hinggan League over time, indicating that the more conflict zone was concentrated in the eastern plain. The severe SCLU evolved from the point to the regional distribution, and from the decentralized to centralized distribution. Correspondingly, three concentrated distribution areas then formed a severe conflict. 4) Some recommendations were given to gradually regulate and control the SCLU during this time. Since the transformation of grassland into cultivated land was the main type of land use change in the severe conflict zone, it was proper to rationally adjust the structure of grassland and cultivated land, in order to alleviate the spatial conflict for the coordinated development of agriculture and animal husbandry. [ABSTRACT FROM AUTHOR]