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154. Antrodia cinnamomeaextract alleviatesbleomycin-induced pulmonary fibrosis in mice by inhibiting the mTOR pathway

Author

Lan, Ying-Wei, Chen, Chia-En, Huang, Tsung-Teng, Huang, Tse-Hung, Chen, Chuan-Mu, and Chong, Kowit-Yu

Abstract

Pulmonary fibrosis is a progressive diffuse parenchymal lung disorder with a high mortality rate. Studies have indicated that injured lung tissues release various pro-inflammatory factors, and produce a large amount of nitric oxide. There is also accumulation of collagen and oxidative stress-induced injury, collectively leading to pulmonary fibrosis. Antrodia cinnamomeais an endemic fungal growth in Taiwan, and its fermented extracts exert anti-inflammatory effects to alleviate liver damages. Hence, we hypothesized and tested the feasibility of using A. cinnamomeaextracts for treatment of pulmonary fibrosis.

Published
2024
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156. Cigarette smoke enhances oncogene addiction to c‐ MET and desensitizes EGFR ‐expressing non‐small cell lung cancer to EGFR TKI s

Author

Tu, Chih‐Yen, primary, Cheng, Fang‐Ju, additional, Chen, Chuan‐Mu, additional, Wang, Shu‐Ling, additional, Hsiao, Yu‐Chun, additional, Chen, Chia‐Hung, additional, Hsia, Te‐Chun, additional, He, Yu‐Hao, additional, Wang, Bo‐Wei, additional, Hsieh, I‐Shan, additional, Yeh, Yi‐Lun, additional, Tang, Chih‐Hsin, additional, Chen, Yun‐Ju, additional, and Huang, Wei‐Chien, additional

Published
2018
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162. Methylation-silencing RCC1 expression is associated with tumorigenesis and depth of invasion in gastric cancer

Author

Lin, Yi-Ling, Chen, Hsiao-Ling, Cheng, Shao-Bin, Yeh, Dah-Cherng, Huang, Chu-Chun, P’eng, Fang-Ku, Tsai, Tung-Chou, Wu, Cheng-Chung, and Chen, Chuan-Mu

Subjects
Male, Carcinogenesis, Molecular Sequence Data, Down-Regulation, Cell Cycle Proteins, Transfection, Cell Movement, Stomach Neoplasms, Cell Line, Tumor, Guanine Nucleotide Exchange Factors, Humans, Neoplasm Invasiveness, Gene Silencing, RNA, Messenger, Base Sequence, Reverse Transcriptase Polymerase Chain Reaction, Tumor Suppressor Proteins, Carcinoma, Nuclear Proteins, Cell Differentiation, DNA Methylation, Middle Aged, Immunohistochemistry, digestive system diseases, Gene Expression Regulation, Neoplastic, Tissue Array Analysis, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Original Article, CpG Islands, Female, Neoplasm Grading, Signal Transduction
Abstract

Introduction: Regulator of chromosome condensation 1 (RCC1) is a critical cell cycle regulator. We firstly identified RCC1 gene hypermethylation in gastric tumor tissues using the differential methylation hybridization (DMH) microarray, but the role of RCC1 in the pathogenesis of gastric carcinoma is largely unknown. Methods: Three gastric cancer cell lines (AGS, MKN45, and TSGH9201) were used to analyze RCC1 gene methylation, mRNA and protein expressions. Furthermore, 85 pairs of matched human gastric carcinoma samples in a tissue microarray were used to analyze RCC1 expression by immunohistochemistry staining. Results: A differential methylation pattern was found in TSGH9201 (100%), MKN45 (87%), and AGS (62%) cell lines at the 9th CpG site of RCC1 exon 1. RCC1 mRNA and protein expressions in AGS cells were significantly higher than in TSGH9201 and MKN45 cell lines (P < 0.05). Tissue array data showed that RCC1 expression was detected in 21% (18/85) of gastric carcinoma tissues and in 80% (76/95) of adjacent non-tumor tissues. The expression of RCC1 in gastric carcinoma tissues was significantly lower than in adjacent non-tumor tissues (P < 0.001). Furthermore, an association between RCC1 expression and clinicopathological features showed that RCC1 expression was closely correlated with tumor differentiation and depth of invasion (P < 0.05). Conclusions: Our data indicate that RCC1 expression is frequently lost in poorly differentiated gastric cell lines and gastric carcinoma tissues. Loss of RCC1 expression is correlated with tumor differentiation and depth of invasion. These findings suggest that RCC1 may play a tumor suppressor role in gastric carcinoma.

Published
2015

174. Anti‐Inflammatory Effects of Antrodia camphorata and its Active Compound, Ergostatrien‐3β‐ol, in a Mouse Skin Ischemia Model

Author

Liao Jiunn-Wang, Tung Yu-Tang, kuo Yueh-Hsiung, Chong Kowit-Yu, Tsai Hsin-Chung, Tsai Tung-Chou, Chen Chuan-Mu, and Chen Hsiao-Ling

Subjects
biology, medicine.drug_class, Chemistry, Ischemia, Pharmacology, medicine.disease, biology.organism_classification, Biochemistry, Anti-inflammatory, Active compound, Mouse skin, Genetics, medicine, Antrodia, Molecular Biology, Biotechnology
Published
2015

178. Interleukin-13 Inhibits Lipopolysaccharide-Induced BPIFA1 Expression in Nasal Epithelial Cells

Author

Tsou, Yung-An, primary, Lin, Chia-Der, additional, Chen, Hui-Chen, additional, Hsu, Hui-Ying, additional, Wu, Lii-Tzu, additional, Chiang-Ni, Chuan, additional, Chen, Chih-Jung, additional, Wu, Tsu-Fang, additional, Kao, Min-Chuan, additional, Chen, Yu-An, additional, Peng, Ming-Te, additional, Tsai, Ming-Hsui, additional, Chen, Chuan-Mu, additional, and Lai, Chih-Ho, additional

Published
2015
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179. Association of β-Lactam-SensitiveHaemophilus influenzaeType B with Adenoid Biofilm Formation in Patients with Adenoidectomy Surgery

Author

Tsou, Yung-An, primary, Lin, Chia-Der, additional, Hsu, Hui-Ying, additional, Peng, Ming-Te, additional, Kuo, Yu-Yi, additional, Tien, Ni, additional, Li, Ju-Pi, additional, Wang, Chien-Kuo, additional, Wu, Hua-Shan, additional, Tsai, Ming-Hsui, additional, Chen, Chuan-Mu, additional, and Lai, Chih-Ho, additional

Published
2015
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181. Molecular Mechanisms of Treadmill Therapy on Neuromuscular Atrophy Induced via Botulinum Toxin A

Author

Tsai, Sen-Wei, Chen, Hsiao-Ling, Chang, Yi-Chun, and Chen, Chuan-Mu

Subjects
Article Subject
Abstract

Botulinum toxin A (BoNT-A) is a bacterial zinc-dependent endopeptidase that acts specifically on neuromuscular junctions. BoNT-A blocks the release of acetylcholine, thereby decreasing the ability of a spastic muscle to generate forceful contraction, which results in a temporal local weakness and the atrophy of targeted muscles. BoNT-A-induced temporal muscle weakness has been used to manage skeletal muscle spasticity, such as poststroke spasticity, cerebral palsy, and cervical dystonia. However, the combined effect of treadmill exercise and BoNT-A treatment is not well understood. We previously demonstrated that for rats, following BoNT-A injection in the gastrocnemius muscle, treadmill running improved the recovery of the sciatic functional index (SFI), muscle contraction strength, and compound muscle action potential (CMAP) amplitude and area. Treadmill training had no influence on gastrocnemius mass that received BoNT-A injection, but it improved the maximal contraction force of the gastrocnemius, and upregulation of GAP-43, IGF-1, Myo-D, Myf-5, myogenin, and acetylcholine receptor (AChR) subunits α and β was found following treadmill training. Taken together, these results suggest that the upregulation of genes associated with neurite and AChR regeneration following treadmill training may contribute to enhanced gastrocnemius strength recovery following BoNT-A injection.

Published
2013
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186. Lung tumorigenesis induced by human vascular endothelial growth factor (hVEGF)-A165 overexpression in transgenic mice and amelioration of tumor formation by miR-16

Author

Tung, Yu-Tang, primary, Huang, Pin-Wu, additional, Chou, Yu-Ching, additional, Lai, Cheng-Wei, additional, Wang, Hsiu-Po, additional, Ho, Heng-Chien, additional, Yen, Chih-Ching, additional, Tu, Chih-Yen, additional, Tsai, Tung-Chou, additional, Yeh, Dah-Cherng, additional, Wang, Jiun-Long, additional, Chong, Kowit-Yu, additional, and Chen, Chuan-Mu, additional

Published
2015
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191. Telomere Elongation and Naive Pluripotent Stem Cells Achieved from Telomerase Haplo-Insufficient Cells by Somatic Cell Nuclear Transfer

Author

Sung, Li-Ying, primary, Chang, Wei-Fang, additional, Zhang, Qian, additional, Liu, Chia-Chia, additional, Liou, Jun-Yang, additional, Chang, Chia-Chun, additional, Ou-Yang, Huan, additional, Guo, Renpeng, additional, Fu, Haifeng, additional, Cheng, Winston T.K., additional, Ding, Shih-Torng, additional, Chen, Chuan-Mu, additional, Okuka, Maja, additional, Keefe, David L., additional, Chen, Y. Eugene, additional, Liu, Lin, additional, and Xu, Jie, additional

Published
2014
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195. Wnt5A regulates ABCB1 expression in multidrug-resistant cancer cells through activation of the non-canonical PKA/β-catenin pathway

Author

Hung, Tsai-Hsien, primary, Hsu, Sheng-Chi, additional, Cheng, Ching-Yi, additional, Choo, Kong-Bung, additional, Tseng, Ching-Ping, additional, Chen, Tse-Ching, additional, Lan, Ying-Wei, additional, Huang, Tsung-Teng, additional, Lai, Hsin-Chih, additional, Chen, Chuan-Mu, additional, and Chong, Kowit-Yu, additional

Published
2014
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197. Staged protocol for the treatment of chronic femoral shaft osteomyelitis with Ilizarov's technique followed by the use of intramedullary locked nail.

Author

Chou, Po-Hsin, Lin, Hsi-Hsien, Su, Yu-Pin, Chiang, Chao-Ching, Chang, Ming-Chau, and Chen, Chuan-Mu

Subjects
OSTEOMYELITIS, FEMUR abnormalities, FEMUR injuries, OSTEOGENESIS imperfecta, OSTEOTOMY
Abstract

Background Infected nonunion of the femoral shaft is uncommon, and usually presents with challenging therapeutic and reconstructive problems. There are still controversies over treating infected nonunion of the femoral shaft. The purposes of this retrospective study were to review the treatment outcomes and describe a staged protocol for spontaneous wound healing. Methods Six patients with chronic femoral shaft infected-nonunion from October 2002 to September 2010 were included in this retrospective study. Serial plain films and triple films of lower legs were performed to evaluate the alignment of the treated femoral shaft and bony union following our staged protocol of Ilizarov distraction osteogenesis and intramedullary nailing. Results An average bone defect of 7 cm was noted after staged osteotomy. Mean follow-up was 87.5 (range, 38–133) months. Union was achieved in all six patients, with an average external fixation time of 6.8 (range, 5–11) months. There was no reinfection. One complication of a 4-cm leg discrepancy was noted, with an initial shortening of 15 cm. The mean knee ranges of motion (ROM) before staged protocols and at final follow-up were 64.2 ± 8.6 (range, 60–75)° and 53.3 ± 9.3 (range, 40–65)°, respectively. The ROM at the knee joint statistically decreased following staged protocols. Conclusion In the treatment of chronic femur osteomyelitis, the staged protocol of Ilizarov distraction osteogenesis followed by intramedullary nailing was safe and successful, and allowed for union, realignment, reorientation, and leg-length restoration. With regard to the soft tissue, this technique provides a unique type of reconstructive closure for infected wounds. It is suggested that the staged protocol is reliable in providing successful simultaneous reconstruction for bone and soft tissue defects without flap coverage. [ABSTRACT FROM AUTHOR]

Published
2017
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198. Lactoferrin interacts with SPLUNC1 to attenuate lipopolysaccharide-induced inflammation of human nasal epithelial cells via down-regulated MEK1/2-MAPK signaling.

Author

Tsou, Yung-An, Tung, Yu-Tong, Wu, Tsu-Fang, Chang, Gary Ro-Lin, Chen, Han-Chien, Lin, Chia-Der, Lai, Chih-Ho, Chen, Hsiao-Ling, and Chen, Chuan-Mu

Subjects
LACTOFERRIN, LIPOPOLYSACCHARIDES, EPITHELIAL cells, MITOGEN-activated protein kinases, EPITHELIUM
Abstract

Copyright of Biochemistry & Cell Biology is the property of Canadian Science Publishing and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2017
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200. Using Dual Fluorescence Reporting Genes to Establish an In Vivo Imaging Model of Orthotopic Lung Adenocarcinoma in Mice.

Author

Lai, Cheng-Wei, Chen, Hsiao-Ling, Yen, Chih-Ching, Wang, Jiun-Long, Yang, Shang-Hsun, and Chen, Chuan-Mu

Subjects
DUAL fluorescence, ADENOCARCINOMA, IN vivo studies, MOUSE diseases, NEAR infrared radiation, LUNG cancer diagnosis, DIAGNOSIS, CARCINOGENESIS, ANIMAL experimentation, BIOLOGICAL models, CONNECTIVE tissues, DIAGNOSTIC imaging, GENES, GENETICS, LIGHT, LUNGS, LUNG tumors, MICE, MOLECULAR diagnosis
Abstract

Purpose: Lung adenocarcinoma is characterized by a poor prognosis and high mortality worldwide. In this study, we purposed to use the live imaging techniques and a reporter gene that generates highly penetrative near-infrared (NIR) fluorescence to establish a preclinical animal model that allows in vivo monitoring of lung cancer development and provides a non-invasive tool for the research on lung cancer pathogenesis and therapeutic efficacy.Procedures: A human lung adenocarcinoma cell line (A549), which stably expressed the dual fluorescence reporting gene (pCAG-iRFP-2A-Venus), was used to generate subcutaneous or orthotopic lung cancer in nude mice. Cancer development was evaluated by live imaging via the NIR fluorescent signals from iRFP, and the signals were verified ex vivo by the green fluorescence of Venus from the gross lung. The tumor-bearing mice received miR-16 nucleic acid therapy by intranasal administration to demonstrate therapeutic efficacy in this live imaging system.Results: For the subcutaneous xenografts, the detection of iRFP fluorescent signals revealed delicate changes occurring during tumor growth that are not distinguishable by conventional methods of tumor measurement. For the orthotopic xenografts, the positive correlation between the in vivo iRFP signal from mice chests and the ex vivo green fluorescent signal from gross lung tumors and the results of the suppressed tumorigenesis by miR-16 treatment indicated that lung tumor size can be accurately quantified by the emission of NIR fluorescence. In addition, orthotopic lung tumor localization can be accurately visualized using iRFP fluorescence tomography in vivo, thus revealing the trafficking of lung tumor cells.Conclusions: We introduced a novel dual fluorescence lung cancer model that provides a non-invasive option for preclinical research via the use of NIR fluorescence in live imaging of lung. [ABSTRACT FROM AUTHOR]

Published
2016
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