Search

Your search keyword '"Chen, Ligang"' showing total 1,249 results
Start Over Author "Chen, Ligang"
1,249 results on '"Chen, Ligang"'

156. Toll‐like receptor 4‐mediated microglial inflammation exacerbates early white matter injury following experimental subarachnoid hemorrhage.

Author

Peng, Jianhua, Xie, Yuke, Pang, Jinwei, Wu, Yue, Zhou, Jian, Gu, Long, Guo, Kecheng, Zhang, Lifang, Xie, Bingqing, Yin, Shigang, Sun, Xiaochuan, Chen, Ligang, and Jiang, Yong

Subjects
WHITE matter (Nerve tissue), SUBARACHNOID hemorrhage, TOLL-like receptors, MICROGLIA, MYELIN basic protein, OLIGODENDROGLIA, VOXEL-based morphometry
Abstract

Neuroinflammation has been reported to be associated with white matter injury (WMI) after subarachnoid hemorrhage (SAH). As the main resident immune cells of the brain, microglia can be activated into proinflammatory and anti‐inflammatory phenotypes. Toll‐like receptor 4 (TLR4), expressed on the surface of the microglia, plays a key role in microglial inflammation. However, the relationship between TLR4, microglial polarization, and WMI following SAH remains unclear. In this study, a total of 121 male adult C57BL/6 wild‐type (WT) mice, 20 WT mice at postnatal day 1 (P1), and 41 male adult TLR4 gene knockout (TLR4−/−) mice were used to investigate the potential role of TLR4‐induced microglial polarization in early WMI after SAH by radiological, histological, microstructural, transcriptional, and cytological evidence. The results indicated that microglial inflammation was associated with myelin loss and axon damage, shown as a decrease in myelin basic protein (MBP), as well as increase in degraded myelin basic protein (dMBP) and amyloid precursor protein (APP). Gene knockout of TLR4 revised microglial polarization toward the anti‐inflammatory phenotype and protected the white matter at an early phase after SAH (24 h), as shown through reduction of toxic metabolites, preservation of myelin, reductions in APP accumulation, reductions in white matter T2 hyperintensity, and increases in FA values. Cocultures of microglia and oligodendrocytes, the cells responsible for myelin production and maintenance, were established to further elucidate the relationship between microglial polarization and WMI. In vitro, TLR4 inhibition decreased the expression of microglial MyD88 and phosphorylated NF‐κB, thereby inhibiting M1 polarization and mitigating inflammation. Decrease in TLR4 in the microglia increased preservation of neighboring oligodendrocytes. In conclusion, microglial inflammation has dual effects on early WMI after experimental SAH. Future explorations on more clinically relevant methods for modulating neuroinflammation are warranted to combat stroke with both WMI and gray matter destruction. [ABSTRACT FROM AUTHOR]

Published
2023
Full Text
View/download PDF

174. Artemisinin attenuated ischemic stroke induced cell apoptosis through activation of ERK1/2/CREB/BCL-2 signaling pathway in vitro and in vivo

Author

Peng , Tangming, Li, Shuai, Liu, Linlin, Yang, Chao, Su, Qiaozhu, Farhan, Mohd, Chen, Ligang, and Zheng, Wenhua

Subjects
Cell Biology, Molecular Biology, Applied Microbiology and Biotechnology, Ecology, Evolution, Behavior and Systematics, Developmental Biology
Abstract

Ischemic stroke is characterized by the presence of both brain ischemic and reperfusion-induced injuries in the brain, leading to neuronal dysfunction and death. Artemisinin, an FDA-approved antimalarial drug, has been reported to have neuroprotective properties. However, the effect of artemisinin on ischemic stroke is not known. In the present study, we investigated the effect of artemisinin on ischemic stroke using an oxygen-glucose deprivation/reperfusion (OGD/RP) cellular model and a mouse middle cerebral artery occlusion (MCAO) animal model and examined the underlying mechanisms. The obtained results revealed that a subclinical antimalarial concentration of artemisinin increased cell viability and decreased LDH release and cell apoptosis. Artemisinin also attenuated the production of reactive oxygen species (ROS) and the loss of mitochondrial membrane potential (Δψm). Importantly, artemisinin attenuated the infarction volume and the brain water content in the MCAO animal model. Artemisinin also improved neurological and behavioural outcomes and restored grasp strength and the recovery of motor function in MCAO animals. Furthermore, artemisinin treatment significantly inhibited the molecular indices of apoptosis, oxidative stress and neuroinflammation and activated the ERK1/2/CREB/BCL-2 signaling pathway. Further validation of the involved signaling pathway by the ERK1/2 inhibitor PD98059 revealed that inhibiting the ERK1/2 signaling pathway or silencing ERK1/2 reversed the neuroprotective effects of artemisinin. These results indicate that artemisinin provides neuroprotection against ischemic stroke via the ERK1/2/CREB/BCL-2 signaling pathway. Our study suggests that artemisinin may play an important role in the prevention and treatment of stroke.

Published
2022

183. Parallel Solution in Simulation of Cardiac Excitation Anisotropic Propagation

Author

Zhang, Yu, Xia, Ling, Gong, Yinglan, Chen, Ligang, Hou, Guanghuan, Tang, Min, Hutchison, David, editor, Kanade, Takeo, editor, Kittler, Josef, editor, Kleinberg, Jon M., editor, Mattern, Friedemann, editor, Mitchell, John C., editor, Naor, Moni, editor, Nierstrasz, Oscar, editor, Rangan, C. Pandu, editor, Steffen, Bernhard, editor, Sudan, Madhu, editor, Terzopoulos, Demetri, editor, Tygar, Doug, editor, Vardi, Moshe Y., editor, Weikum, Gerhard, editor, Sachse, Frank B., editor, and Seemann, Gunnar, editor

Published
2007
Full Text
View/download PDF

184. An Improved Laplacian Smoothing Approach for Surface Meshes

Author

Chen, Ligang, Zheng, Yao, Chen, Jianjun, Liang, Yi, Hutchison, David, editor, Kanade, Takeo, editor, Kittler, Josef, editor, Kleinberg, Jon M., editor, Mattern, Friedemann, editor, Mitchell, John C., editor, Naor, Moni, editor, Nierstrasz, Oscar, editor, Rangan, C. Pandu, editor, Steffen, Bernhard, editor, Sudan, Madhu, editor, Terzopoulos, Demetri, editor, Tygar, Doug, editor, Vardi, Moshe Y., editor, Weikum, Gerhard, editor, Shi, Yong, editor, van Albada, Geert Dick, editor, Dongarra, Jack, editor, and Sloot, Peter M. A., editor

Published
2007
Full Text
View/download PDF

200. REGIONAL CLIMATE–WEATHER RESEARCH AND FORECASTING MODEL

Author

Liang, Xin-Zhong, Xu, Min, Yuan, Xing, Ling, Tiejun, Choi, Hyun I., Zhang, Feng, Chen, Ligang, Liu, Shuyan, Su, Shenjian, Qiao, Fengxue, He, Yuxiang, Wang, Julian X. L., Kunkel, Kenneth E., Gao, Wei, Joseph, Everette, Morris, Vernon, Yu, Tsann-Wang, Dudhia, Jimy, and Michalakes, John

Published
2012

Catalog

Books, media, physical & digital resources
See catalog results