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Acute myeloid leukemia (AML) is a complex and heterogeneous hematopoietic tissue neoplasm. Several molecular markers have been described that help to classify AML patients into risk groups. DNA methyltransferase 3A (DNMT3A) gene mutations have been recently identified in about 22% of AML patients and associated with poor prognosis as an independent risk factor. Our aims were to determine the frequency of somatic mutations in the gene DNMT3A and major chromosomal translocations in a sample of patients with AML. We investigated in 82 samples of bone marrow from patients with AML for somatic mutations in DNMT3A gene by sequencing and sought major fusion transcripts by RT-PCR. We found mutations in the DNMT3A gene in 6 patients (8%); 3 were type R882H. We found fusion transcripts in 19 patients, namely, AML1/ETO (n=5; 6.1%), PML/RARα (n=12; 14.6%), MLL/AF9 (0; 0%), and CBFβ/MYH11 (n=2; 2.4%). The identification of recurrent mutations in the DNMT3A gene and their possible prognostic implications can be a valuable tool for making treatment decisions. This is the first study on the presence of somatic mutations of the DNMT3A gene in patients with AML in Brazil. The frequency of these mutations suggests a possible ethnogeographic variation.

Leishmania major-infected TNF receptor 1 deficient (TNFR1 KO) mice resolve parasitism but fail to resolve lesions, while wild-type mice completely heal. We investigated the cell composition, cytokine production, and apoptosis in lesions from L. major-infected TNFR1 KO and wild-type (WT) mice. Chronic lesions from L. major-infected TNFR1 KO mice presented larger number of CD8+ T and Ly6G+ cells. In addition, higher concentrations of mRNA for IFN-γ CCL2 and CCL5, as well as protein, but lower numbers of apoptotic cells, were found in lesions from TNFR1 KO mice than in WT, at late time points of infection. Our studies showed that persistent lesions in L. major-infected TNFR1 KO mice may be mediated by continuous migration of cells to the site of inflammation due to the presence of chemokines and also by lower levels of apoptosis. We suggest that this model has some striking similarities to the mucocutaneous clinical form of leishmaniasis.

Background: Despite some advances in the discovery of novel therapies, smoke inhalation injury remains a difficult to treat critical health issue due to its physiopathological complexity. Natural products, such as D-limonene (LIM), are becoming an important potential source of new treatments in many health problems. LIM has been shown to have antioxidant, anti-inflammatory and protective effects in respect of several diseases, including respiratory conditions. Objective: The aim of this study was to evaluate the effects of inhaled LIM on acute smoke-induced lung injury in rats. Methods: Thirty minutes after smoke inhalation, adult male Wistar rats were treated with vehicle or LIM (0.01 mg/kg) for 30 min. Blood samples, and the liver, lungs, and trachea were collected for analysis. Results: The results showed that LIM minimized the injuries, reducing oxidative and inflammatory damage by improving catalase (CAT) (p < 0.05), and superoxide dismutase (SOD) activities (trachea: p < 0.01; lung: p < 0.05) and reducing interleukin-1 beta (IL-1β) level (p < 0.01) caused by smoke inhalation. LIM was also able to ameliorate damage in both trachea and lung tissues. Conclusions: The results indicate that LIM has a beneficial effect on lung injury, mainly by reducing oxidative stress, anti-inflammatory response and tissue damage.

O artigo analisa as associações negras pelotenses que permitiram a constituição de uma identidade negra positiva percebida em relação direta com o contexto relacional vivenciado em âmbito nacional e internacional. Destacamos ainda a busca por um entrelaçamento entre diferentes associações tendo como ponto em comum a identificação racial mediada pela busca por uma posição social diferenciada. Diretamente vinculado ao período do pós-Abolição almejava-se a cidadania em uma sociedade ainda muito preconceituosa racialmente. A delimitação espacial focaliza o estudo na cidade de Pelotas, localizada no extremo sul do Rio Grande do Sul, entre os anos de 1820 a 1943. Tem inicio com a criação da primeira irmandade negra em 1820. Dentre as associações destacamos o surgimento dos clubes sociais negros (1917-1931) e suas atividades em conjunto, com ênfase para o surgimento da Frente Negra Pelotense (1933) e a busca por uma identidade racial que fosse comungada por todo o grupo negro local.

Objetivou-se determinar as necessidades de proteína e energia líquida de ovelhas em lactação submetidas a dois manejos nutricionais na gestação e dois tipos de gestação. Os ensaios experimentais foram conduzidos nas instalações do Laboratório de Metabolismo e Calorimetria Animal do Departamento de Zootecnia da Escola de Veterinária da UFMG. Foram utilizadas 28 ovelhas da raça Santa Inês, em um delineamento experimental inteiramente casualizado em arranjo fatorial 2x2, e considerou-se dois tipos de gestação (simples e gemelar) e dois tipos de manejo nutricional (com e sem restrição). As ovelhas eram colocadas sobre plataforma e contidas para realização da ordenha. Para análise dos dados referentes às semanas em lactação utilizou-se o modelo de medidas repetidas no tempo. Os grupos experimentais foram avaliados mediante utilização do teste SNK a 5% de significância. A produção de leite não foi afetada pelo manejo nutricional, bem como a quantidade de gordura, proteína, energia e as necessidades em energia e proteína. Portanto, ficou evidenciado neste estudo que somente o manejo nutricional não foi capaz de alterar as variáveis estudadas. Ovelhas com gestação dupla apresentaram maior produção de leite, maiores teores de gordura e proteína no leite bem como maior valor energético do mesmo. As necessidades em energia e proteína foram maiores para ovelhas com gestação dupla. A produção de leite e as necessidades em energia e proteína foram maiores até a décima semana de lactação. A exigência nutricional em energia e proteína acompanhou a curva de produção de leite.This study aimed to obtain the protein and liquid energy requirements from lactating ewes under two nutritional managements during pregnancy and under two types of pregnancy. Experimental tests were conducted at the Animal Metabolism and Calorimetry Laboratory on Department of Animal Science from Veterinary School of UFMG. For the experimental testing, were used 28 Santa Ines sheeps in a completely randomized design under 2 x 2 factorial arrangement, considering two pregnancy types (single and double) and two nutritional managements (with and without restriction). Sheeps were placed on the platform and contained for milking. To analyse data regarding milking weeks it was used the model of repeated measures in time. The experimental groups were evaluated using the SNK test at 5% significance level. (1) Diets were compound of corn meal (Zea mays), soybean meal (Glycine max), chopped Tifton grass hay (Cynodon spp.) and limestone. Sheep’s specific mineralsupplement was offered ad libitum to the animals Milk production was not affected by nutritional management, as the amount of fat, protein and, energy from milk. The same results were observed to energy and protein requirements. This fact shows that the nutritional management, per se, was not able to change the variables studied. The double pregnancy produced more milk, fat and protein in milk increasing its energy value. Energy and protein requirements were higher in ewes with double pregnancy. Milk production, energy and protein requirements were higher until the tenth week of lactation. Energy and protein nutritional requirement followed the milk production curve.

Trata-se de um livro recentemente publicado, onde as informações, análises e as coclusões apresentadas revelam o compretimento das autoras em compartilhar conhecimentos coletivos adquiridos através da arte da investigação.

Nos últimos anos, no sertão do estado do Ceará, tem ocorrido mudanças nas relações de gênero. Mulheres que se descobrem líderes têm transformado a história de suas comunidades e contribuído para o despertar de uma nova cultura. Documentar a realidade destas mulheres, usando a fotografia como registro antropológico, é o objetivo deste trabalho. Ele documenta experiências de mulheres líderes nos assentamentos do PDHC – Projeto Dom Hélder Câmara na cidade de Quixeramobim, no Sertão Central do Ceará.

A monitorização das complicações no decurso da VNI desempenha um papel fundamental na evolução favorável com esta terapêutica.Para avaliar a correlação entre o insucesso da VNI em doentes com DPOC descompensada com acidémia respiratória grave (pH

A utilização da VNI tem aumentado na última década em doentes com insuficiência respiratória agudizada. Pretendeu-se avaliar o sucesso da VNI em doentes com acidémia respiratória grave (pH

Dengue virus serotype 3 (DENV-3) consists of 5 distinct genotypes (I-V). Genotype III (GIII) is the most widespread and was associated with large outbreaks in Asia, Africa, and the Americas [...]

The aim of this study was to compare radiographic and clinical status and peri-implant sulcular fluid (PISF) prostaglandin E2 (PGE2) levels among patients with and without peri-implant disease. Patients with peri-implant mucositis (PiM) (Group 1) and peri-implantitis (Group 2) and individuals without peri-implant disease (Group 3) were included. Demographic information was collected, and peri-implant modified plaque and bleeding indices (mPI and mBI), probing depth (PD), and crestal bone loss (CBL) were recorded. PISF samples were collected and PGE2 levels measured. Statistical significance was set at P < .01. A total of 22 patients with PiM, 22 with peri-implantitis, and 23 without peri-implant disease (controls) were included. Scores of mPI (P < .01), mBI (P < .01), and PD (P < .01) were higher in patients with PiM and peri-implantitis than the controls. The volumes of collected PISF were significantly higher in patients with peri-implantitis (P < .01) compared to patients with PiM and the controls. The PISF volume was significantly higher in PiM patients (P < .01) than in the controls. There was a significant correlation between peri-implant PD and PISF PGE2 levels in patients with peri-implantitis (P < .001). Raised PISF PGE2 levels indicate poor peri-implant health; therefore, PGE2 is a potential biomarker for the assessment of peri-implant health status. [ABSTRACT FROM AUTHOR]