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942 results on '"Fujisaki, K."'

152. Semi-continuous oscillation of halide copper vapor laser

Author

Tawada, Fuyuki, primary, Hisida, Tomohiro, additional, Sugiyama, Kentarou, additional, Yukawa, Yosinari, additional, Uno, Katuhiro, additional, Fujii, Kan-ichi, additional, Matsushita, Shingo, additional, Yoshida, Kenji, additional, Endo, Masamori, additional, Muto, Shigeki, additional, Fukuoka, Shuji, additional, Fujioka, Tomoo, additional, Nanri, Kenzo, additional, Okamura, M., additional, Yoshitani, E., additional, Fujisaki, K., additional, Kihara, Y., additional, Fujii, H., additional, Tsunekane, M., additional, Mukaihara, K., additional, Morita, H., additional, Ishida, T., additional, Suzuki, Y., additional, Konno, Susumu, additional, Fujikawa, Shuichi, additional, Yasui, Koji, additional, Furuta, K., additional, Kojima, Tetsuo, additional, Nakayama, M., additional, Yuasa, H., additional, Lu, J, additional, Murai, T., additional, Takaichi, K., additional, Uematsu, T., additional, Misawa, K., additional, Mizuno, K., additional, Ueda, K., additional, Yagi, H., additional, Yanagitani, T., additional, Kaminskii, A. A., additional, Hirose, Shouji, additional, Sekiguchi, Tomoki, additional, Xu, Jianqiu, additional, Kurita, T., additional, Kanabe, T., additional, Kawashima, T., additional, Tamaoki, Y., additional, Yamanaka, M., additional, Nakatsuka, M., additional, Izawa, Y., additional, Nakai, S., additional, Kawada, Yasuo, additional, Kato, Yoshinori, additional, Watanabe, Takashi, additional, Yamanaka, Chiyoe, additional, Kamiya, Takahiro, additional, Kasuya, Koichi, additional, Fukuda, Kengo, additional, Hayashi, Nobuya, additional, Satoh, Saburoh, additional, Yamabe, Chobei, additional, Shirai, T., additional, Tabe, Y., additional, Kubodera, S., additional, Kawanaka, J., additional, Sasaki, W., additional, Igarashi, T., additional, Yabu, Takayuki, additional, Nagai, Shinji, additional, Ochiishi, Yoshinori, additional, Hisanaga, Naoto, additional, Ohbu, Ayako, additional, Fujimoto, Junichi, additional, Mizoguchi, Hakaru, additional, Ueno, Y., additional, Kumazaki, T., additional, Ariga, T., additional, Nishisaka, T., additional, Nohdomi, R., additional, Watanabe, H., additional, Kitatochi, N., additional, Hoshino, H., additional, Kodama, Y., additional, Takahashi, K., additional, Sugimoto, Y., additional, Abe, T., additional, Sajiki, K., additional, Nire, T., additional, Miura, T., additional, Takasago, K., additional, Torizuka, K., additional, Kannari, F., additional, Hori, Takashi, additional, Nishizawa, Norihiko, additional, Goto, Toshio, additional, Yoshida, Makoto, additional, Oishi, Y., additional, Suda, A., additional, Takatoi, K., additional, Wang, P., additional, Nagasaka, K., additional, Midorikawa, K., additional, Nakano, Fumihiko, additional, Kase, Teiji, additional, Akahane, Yutaka, additional, Aoyama, Makoto, additional, Asabe, Kaoru, additional, Yamakawa, Koichi, additional, HASEGAWA, Yoshihisa, additional, HIBI, Megumi, additional, FUSEYA, Takafumi, additional, YOSHIDA, Hiroki, additional, SAGAMI, Yukio, additional, Asakawa, Yuichi, additional, Kumagai, Hiroshi, additional, Obara, Minoru, additional, Saito, Norihito, additional, Wada, Satoshi, additional, Tashiro, Hideo, additional, Wang, Yimin, additional, NAKANO, Yoko, additional, SHIMIZU, Takayuki, additional, MIYAZAKI, Kenzo, additional, Kouta, H., additional, Kuwano, Y., additional, Kasamatsu, T., additional, Sakuma, J., additional, Ueno, T., additional, Yoneda, H., additional, Nagata, H., additional, Ohta, S., additional, Yamamoto, H., additional, Baba, K., additional, Nishioka, Hajime, additional, Fujita, M., additional, Inoue, N., additional, Kubo, R., additional, Kaminoe, T., additional, Ohigashi, N., additional, Tsunawaki, Y., additional, Asakawa, M., additional, Imasaki, K., additional, Mima, K., additional, Masaki, Y., additional, Masuda, M., additional, Tokura, Y., additional, Akita, S., additional, Nakayama, Y., additional, Minehara, EJ., additional, Yamauchi, T., additional, Sugimoto, M., additional, Sawamura, M., additional, Hajima, R., additional, Nagai, R., additional, Kikuzawa, N., additional, Hayakawa, T., additional, Nishimori, N., additional, Shizuma, T., additional, Mori, A., additional, Suzuki, K., additional, Kanari, K., additional, MIYAZAKI, Kazuhiko, additional, Park, Sung-fin, additional, Oh, Chul-Iian, additional, Amano, S., additional, Miyamoto, S., additional, Fukugaki, K., additional, Mochizuki, T., additional, Hashimoto, S., additional, Shouji, Y., additional, Ando, A., additional, Aoki, K., additional, and Hosono, K., additional

Published
2001
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182. Seasonal changes in resource allocation within an individual offspring of the wolf spider, Pardosa pseudoannulata (Araneae: Lycosidae).

Author

IIDA, H. and FUJISAKI, K.

Subjects
*WOLF spiders, *ANIMAL wintering, *BODY size, *EMBRYOLOGY, *PREDATION
Abstract

Seasonal changes in resource investment into individual offspring are well documented, but no attention has been paid to the allocation of the invested resource among the body parts of the offspring. In the present study, seasonal changes in the absolute and relative sizes in a spiderling of the wolf spider Pardosa pseudoannulata Boes. et Str. are investigated, and the relationship between spiderling body size and the ability to moult during a food shortage is clarified. Spiderlings that emerge in November have a significantly larger cephalothorax and abdomen than those that emerge in June. In addition, the abdomen–cephalothorax size ratio is significantly greater in November than in June offspring. Under limited food availability conditions, only 40% of spiderlings moult. Nymphs that do moult have a significantly larger cephalothorax, abdomen and abdomen–cephalothorax size ratio than nymphs that do not moult. Thus, both the quantity of resources invested in the cephalothorax and abdomen of a spiderling and the proportional allocation of resources between the two body parts change seasonally in Pa. pseudoannulata; alteration of the resource allocation occurs in late autumn. Larger spiderlings of Pa. pseudoannulata that emerge in late autumn would be able to develop into advanced instars even during food shortage, and therefore may have better overwintering survival rates. [ABSTRACT FROM AUTHOR]

Published
2007
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183. Graft infection of thoracic aorta due to Group C ß-hemolytic streptococcus: a case report.

Author

Arima H, Nagata M, Fujisaki K, Gushima M, Matsumura K, Fujii K, Tsuchihashi T, Hirakata H, Eguchi T, Moroi Y, Furue M, Yasui H, Tsuneyoshi M, and Iida M

Abstract

A fatal case of late-onset graft infection of the thoracic aorta due to group C beta-hemolytic streptococcus is described. A 37-year-old male patient, who had a history of total aortic arch replacement for acute aortic dissection 8 years before, was admitted to the department. He suffered from toxic shock syndrome, disseminated intravascular coagulation (DIC), and acute renal failure. Group C beta-hemolytic streptococcus was detected from his blood; however, echocardiography, computed tomography (CT), and magnetic resonance imaging (MRI) failed to detect the focus of the infection. In spite of intensive care, including antibiotic therapy, artificial ventilation, and continuous hemodiafiltration, he died on the 18th day of hospitalization. Autopsy revealed that a small abscess was present at the proximal anastomotic segments of the patient's graft. A bite inflicted by his dog, 14 days before admission, was suspected to be the source of this bacterium. A rare case of graft infection of thoracic aorta in terms of causative organism, long period from graft replacement to graft infection, and site of infection is presented and discussed. [ABSTRACT FROM AUTHOR]

Published
2005
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184. Molecular characterization of a peroxiredoxin from the hard tick Haemaphysalis longicornis.

Author

Tsuji, N., Kamio, T., Isobe, T., and Fujisaki, K.

Subjects
ENZYMES, ANTIOXIDANTS, MOLECULAR cloning, HAEMAPHYSALIS longicornis, NUCLEOTIDE sequence, MOLECULAR genetics
Abstract

AbstractAntioxidant enzymes in eukaryotes play an important role in protection against the oxygen radicals generated during aerobic metabolism. Here we report the cloning and characterization of a cDNA encoding the antioxidant enzyme peroxiredoxin from the hard tick Haemaphysalis longicornis (HlPrx). HlPrx is 939 bp long and contains a 101 bp non-translated sequence at the 5′ end and a polyadenylation singnal followed by a poly(A) tail at the 3′ end. HlPrx encodes a full-length protein with a predicted molecular mass of 26 kDa that possesses one cysteine residue at amino acid 49 that is conserved among Prx proteins of various species. GenBank™ analysis showed that the deduced amino acid sequence had significant similarity to mammalian and plant Prxs at the amino acid level. A DNA-nicking assay revealed that Escherichia coli–expressed recombinant HlPrx (rHlPrx) inhibited oxidative-nicking of supercoiled plasmid DNA. Two-dimensional immunoblot analysis with mouse antirHlPrx serum showed reaction with a major constituent protein spot in extracts of adult ticks. In addition, immunoblot analysis showed that rHlPrx was immunoreacted with serum from rabbits repeatedly infested with H. longicornis. Localization analysis using mouse antirHlPrx serum revealed that native HlPrx was highly expressed in the salivary gland of the tick. Moreover, Northern blot analysis showed that the level of HlPrx transcripts was increased during blood sucking. The present results indicate that HlPrx may be an important detoxifying enzyme during the normal life span as well as during blood sucking in ticks. [ABSTRACT FROM AUTHOR]

Published
2001
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185. Antigenic differences between Japanese Theileria sergenti and other benign Theileria species of cattle from Australia ( T. buffeli) and Britain ( T. orientalis).

Author

Kawazu, S., Sugimoto, C., Kamio, T., and Fujisaki, K.

Abstract

Serological comparisons among piroplasm antigens of the benign Theileria species of cattle from Japan, Australia and Britain, which are frequently referred to as T. sergenti, T. buffeli and T. orientalis, were carried out. The results obtained from comparative enzyme-linked immunosorbent assay (ELISA) using sera from infected cattle suggest that T. sergenti could be differentiated from both T. buffeli and T. orientalis by their serological dissimilarities. Western blotting combined with two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) revealed that both the 33-kDa piroplasm protein of T. sergenti and the similar 34-kDa protein of T. buffeli and T. orientalis corresponded to immunodominant antigens against cattle. The other 32-kDa proteins of T. buffeli and T. orientalis also represented immunodominant antigens. Cross-reactivities of the 32-and 34-kDa proteins were observed between T. buffeli and T. orientalis, whereas the 33-kDa protein of T. sergenti could be differentiated from the similar 34-kDa proteins of T. buffeli/orientalis. The present study suggests that T. sergenti should be separated from T. buffeli and T. orientalis on the basis of their serological dissimilarities. [ABSTRACT FROM AUTHOR]

Published
1992
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186. Clotrimazole, ketoconazole, and clodinafop-propargyl inhibit the <e1>in vitro</e1> growth of <e1>Babesia bigemina</e1> and <e1>Babesia bovis</e1> (Phylum Apicomplexa)

Author

BORK, S., YOKOYAMA, N., MATSUO, T., CLAVERIA, F. G., and FUJISAKI, K.

Abstract

We evaluated the growth inhibitory efficacy of the imidazole derivatives, clotrimazole (CLT) and ketoconazole (KC), and the herbicide clodinafop-propargyl (CP), in in vitro cultures of Babesia bovis and B. bigemina. Clotrimazole was effective in a dose range of 15 to 60 μM (IC50: 11 and 23·5 μM), followed by KC (50 to 100 μM; IC50: 50 and 32 μM) and CP (500 μM; IC50: 265 and 390 μM). In transmission electron microscopy, extensive damage was observed in the cytoplasm of drug-treated parasites. Combinations of CLT/KC, CLT/CP and CLT/KC/CP acted synergistically in both parasites. In contrast, the combination of KC/CP was exclusively effective in B. bovis, but not in B. bigemina.

Published
2003

187. Morphological studies on the extracellular structure of the midgut of a tick,Haemaphysalis longicornis(Acari: Ixodidae)

Author

Matsuo, T., Sato, M., Inoue, N., Yokoyama, N., Taylor, D., and Fujisaki, K.

Abstract

In the present study, morphological observations on the extracellular structures found on the apical surface of the midgut epithelium, known as the peritrophic membrane (PM) or glycocalyx, are described inHaemaphysalis longicornisfemales and larvae. These structures have been hypothesized to provide protection to the microvilli of epithelial cells of the digestive tract. Our aim was to determine whether the extracellular structures are important in the digestion of the blood meal and/or as a protection against infection or injury. The PM was detectable in the midgut of engorged larvae by electron microscopy, but not in engorged females. However, a PM-like structure, stainable with toluidine blue, was observed in females by light microscopy. From the results of confocal laser scanning and electron microscopic observations with wheat germ agglutinin (WGA lectin) staining for chitin of the PM, however, the structure was clearly recognized. The structure in the female is likely to be PM because staining with WGA lectin in the presence of GlcNAc indicates the presence of chitin and various morphologies of PM have been reported in insects and ticks. These results show morphologically that different types of PM-like structure are formed in larvae and females ofH. longicornis.

Published
2003
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192. Interferon-gamma-induced apoptosis in host cells infected with <e1>Neospora caninum</e1>

Author

*, Y. NISHIKAWA, †, MISHIMA, M., NAGASAWA, H., IGARASHI, I., FUJISAKI, K., and OTSUKA, H.

Abstract

Interferon-gamma (IFN-γ) has a crucial role for host defence against parasite infection. It is not clear, however, how IFN-γ affects the parasite-infected host cells. The effect of IFN-γ on Neospora caninum-infected cells was investigated in murine fibroblasts and canine kidney cells in vitro. In the presence of IFN-γ, the viability of the infected host cell was decreased and apoptotic cell death occurred, as analysed by DNA stainings with propidium iodide and a terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labelling (TUNEL) and DNA fragmentation. The percentage of apoptotic cells depended on the dose of IFN-γ. Flow cytometric analysis indicated a significant increase of FasL expression on the IFN-γ-treated cells following N. caninum infection. Moreover, IFN-γ treatment down-regulated Bcl-2 expression in the cells cultured with N. caninum while parasite infection up-regulated Bcl-2 expression. The present study suggests that the IFN-γ induced increases of FasL expression and down-regulated Bcl-2 expression in N. caninum-infected cells are associated with apoptosis in vitro.

Published
2001

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