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557 results on '"Gao, Yongxiang"'

160. Identification of the Active Constituents and Significant Pathways of Guizhi-Shaoyao-ZhimuDecoction for the Treatment of Diabetes Mellitus Based on Molecular Docking and Network Pharmacology

Author

Zhang, Qing, Li, Ruolan, Peng, Wei, Zhang, Mengmeng, Liu, Jia, Wei, Shujun, Wang, Jiaolong, Wu, Chunjie, Gao, Yongxiang, and Pu, Xufeng

Abstract

Aim and Objective: This study was designed to explore the active compounds and significant pathways of Guizhi-Shaoyao-Zhimu decoction (GSZD) for treating diabetes mellitus using molecular docking combined with network pharmacology. Materials and Methods: Chemical constituents of GSZD and diabetes-related target proteins were collected from various databases. Then, compounds were filtered by Lipinski’s and Veber’s rules with Discovery studio software. The “Libdock” module was used to carry out molecular docking, and LibDockScores, default cutoff values for hydrogen bonds, and van der Waals interactions were recorded. LibDockScore of the target protein and its prototype ligand was considered as the threshold, and compounds with higher LibDockScores than the threshold were regarded as the active constituents of GSZD. Cytoscape software was used to construct the herb-active molecule-target interaction network of GSZD. ClueGO and CluePedia were applied to enrich the analysis of the biological functions and pathways of GSZD. Results: A total of 275 potential active compounds with 57 possible pathways in GSZD were identified by molecular docking combined with network pharmacology. TEN, INSR, PRKAA2, and GSK3B are the four most important target proteins. Gancaonin E, 3'-(γ,γ-dimethylallyl)-kievitone, aurantiamide, curcumin and 14-O-cinnamoylneoline, could interact with more than 14 of the selected target proteins. Besides, 57 potential pathways of GSZD were identified, such as insulin signaling pathway, metabolites and energy regulation, glucose metabolic process regulation, and positive regulation of carbohydrate metabolic process, etc. Conclusion: These results showed that molecular docking combined with network pharmacology is a feasible strategy for exploring bioactive compounds and mechanisms of Chinese medicines, and GSZD can be used to effectively treat diabetes through multi-components and multi-targets & pathways.

Published
2019
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171. Magnetic and photoactive colloidal shuttles for active cargo transportation

Author

Zhang, Xinyuan, Chen, Zequ, Li, Yurou, Li, Xiyue, Li, Ruiyao, Zhang, Jiayu, Imran, Muhammad, and Gao, Yongxiang

Abstract

Colloidal shuttles are micro/nanoscale motors that display controllable cargo loading/release and programmable navigation, which are emerging delivery vehicles at the small scale. Here we present a hydrogen peroxide-fueled catalytic colloidal shuttle composed of a hematite cube half coated with platinum, i.e. a Pt/hematite Janus cube, which can be remotely controlled with ease by light and magnetic field. Interestingly, the dynamic behaviors of the Pt/hematite motor under light illumination in lower fuel concentration are similar to those Pt-based motors in higher fuel concentration without UV light, including the self-propulsion direction and the interaction with passive particles. In lower fuel concentration, we demonstrate the ability of the Pt/hematite motor for light-switchable cargo loading and release, and programmable and directional transportation of cargoes using the intrinsic magnetic property of hematite. Our work offers an efficient colloidal shuttle that operates at favorable fuel concentration and light intensity in comparison to earlier reported cargo-towing colloidal motors, which should find applications as microscale delivery vehicles, particularly for cargo transportation on microchips.

Published
2023
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175. Structural and biochemical insights into the DNA-binding mode of MjSpt4p:Spt5 complex at the exit tunnel of RNAPII.

Author

Guo, Gongrui, Gao, Yongxiang, Zhu, Zhongliang, Zhao, Debiao, Liu, Zhihong, Zhou, Huihao, Niu, Liwen, and Teng, Maikun

Subjects
*DNA-binding proteins, *RNA polymerases, *TRANSCRIPTION factors, *HETERODIMERS, *ELONGATION factors (Biochemistry), *FLUORESCENCE polarization immunoassay
Abstract

Spt5 (NusG in bacteria) is the only RNA polymerase-associated factor known to be conserved in all three domains of life. In archaea and eukaryotes, Spt5 associates with Spt4, an elongation factor that is absent in bacteria, to form a functional heterodimeric complex. Previous studies suggest that the Spt4:Spt5 complex interacts directly with DNA at the double-stranded DNA exit tunnel of RNA polymerase to regulate gene transcription. In this study, the DNA-binding ability of Spt4:Spt5 from the archaeon Methanocaldococcus jannaschii was confirmed via nuclear magnetic resonance chemical shift perturbation and fluorescence polarization assays. Crystallographic analysis of the full-length Mj Spt4:Spt5 revealed two distinct conformations of the C-terminal KOW domain of Spt5. A similar alkaline region was found on the Spt4:Spt5 surface in both crystal forms, and identified as double-stranded DNA binding patch through mutagenesis-fluorescence polarization assays. Based on these structural and biochemical data, the Spt4:Spt5-DNA binding model was built for the first time. [ABSTRACT FROM AUTHOR]

Published
2015
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180. Chemical inhibition of ArabidopsisPIN-FORMED auxin transporters by the anti-inflammatory drug naproxen

Author

Xia, Jing, Kong, Mengjuan, Yang, Zhisen, Sun, Lianghanxiao, Peng, Yakun, Mao, Yanbo, Wei, Hong, Ying, Wei, Gao, Yongxiang, Friml, Jiří, Weng, Jianping, Liu, Xin, Sun, Linfeng, and Tan, Shutang

Abstract

The phytohormone auxin plays central roles in many growth and developmental processes in plants. Development of chemical tools targeting the auxin pathway is useful for both plant biology and agriculture. Here we reveal that naproxen, a synthetic compound with anti-inflammatory activity in humans, acts as an auxin transport inhibitor targeting PIN-FORMED (PIN) transporters in plants. Physiological experiments indicate that exogenous naproxen treatment affects pleiotropic auxin-regulated developmental processes. Additional cellular and biochemical evidence indicates that naproxen suppresses auxin transport, specifically PIN-mediated auxin efflux. Moreover, biochemical and structural analyses confirm that naproxen binds directly to PIN1 protein via the same binding cavity as the indole-3-acetic acid substrate. Thus, by combining cellular, biochemical, and structural approaches, this study clearly establishes that naproxen is a PIN inhibitor and elucidates the underlying mechanisms. Further use of this compound may advance our understanding of the molecular mechanisms of PIN-mediated auxin transport and expand our toolkit in auxin biology and agriculture.

Published
2023
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