800 results on '"Hien, Tran Tinh"'
Search Results
152. In Reply: Effects of dopamine and adrenaline infusions in severe infection
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Day, Nicholas, Phu, Nguyen Hoan, Bethell, Delia, Hien, Tran Tinh, and White, Nicholas J.
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- 1996
153. Fluoroquinolone antibiotics in children with multidrug resistant typhoid
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White, Nicholas J., Dung, Nguyen Minh, Vinh, Ha, Bethell, Delia, and Hien, Tran Tinh
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- 1996
154. Effects on growth of single short courses of fluoroquinolones
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Bethell, Delia B., Hien, Tran Tinh, Phi, Le Thi, Day, Nicholas P. J., Vinh, Ha, Duong, Nguyen Minh, Van Len, Nguyen, Van Chuong, Ly, and White, Nicholas J.
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- 1996
155. Role of quinine in the high mortality of intramuscular injection tetanus
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Yen, Lam Minh, Dao, Le Minh, Day, Nicholas P J, Waller, Deborah J, Bethell, Delia B, Son, Le Hong, Hien, Tran Tinh, and White, Nicholas J.
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- 1994
156. The Natural History and Transmission Potential of Asymptomatic Severe Acute Respiratory Syndrome Coronavirus 2 Infection.
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Chau, Nguyen Van Vinh, Lam, Vo Thanh, Dung, Nguyen Thanh, Yen, Lam Minh, Minh, Ngo Ngoc Quang, Hung, Le Manh, Ngoc, Nghiem My, Dung, Nguyen Tri, Man, Dinh Nguyen Huy, Nguyet, Lam Anh, Nhat, Le Thanh Hoang, Nhu, Le Nguyen Truc, Ny, Nguyen Thi Han, Hong, Nguyen Thi Thu, Kestelyn, Evelyne, Dung, Nguyen Thi Phuong, Xuan, Tran Chanh, Hien, Tran Tinh, Phong, Nguyen Thanh, and Tu, Tran Nguyen Hoang
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CORONAVIRUS diseases ,LONGITUDINAL method ,POLYMERASE chain reaction ,VIRAL load ,SYMPTOMS ,REVERSE transcriptase polymerase chain reaction ,DESCRIPTIVE statistics ,INFECTIOUS disease transmission - Abstract
Background Little is known about the natural history of asymptomatic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Methods We conducted a prospective study at a quarantine center for coronavirus disease 2019 in Ho Chi Minh City, Vietnam. We enrolled quarantined people with reverse-transcription polymerase chain reaction (RT-PCR)–confirmed SARS-CoV-2 infection, collecting clinical data, travel and contact history, and saliva at enrollment and daily nasopharyngeal/throat swabs (NTSs) for RT-PCR testing. We compared the natural history and transmission potential of asymptomatic and symptomatic individuals. Results Between 10 March and 4 April 2020, 14 000 quarantined people were tested for SARS-CoV-2; 49 were positive. Of these, 30 participated in the study: 13 (43%) never had symptoms and 17 (57%) were symptomatic. Seventeen (57%) participants imported cases. Compared with symptomatic individuals, asymptomatic people were less likely to have detectable SARS-CoV-2 in NTS collected at enrollment (8/13 [62%] vs 17/17 [100%]; P = .02). SARS-CoV-2 RNA was detected in 20 of 27 (74%) available saliva samples (7 of 11 [64%] in the asymptomatic group and 13 of 16 [81%] in the symptomatic group; P = .56). Analysis of RT-PCR positivity probability showed that asymptomatic participants had faster viral clearance than symptomatic participants (P < .001 for difference over the first 19 days). This difference was most pronounced during the first week of follow-up. Two of the asymptomatic individuals appeared to transmit SARS-CoV-2 to 4 contacts. Conclusions Asymptomatic SARS-CoV-2 infection is common and can be detected by analysis of saliva or NTSs. The NTS viral loads fall faster in asymptomatic individuals, but these individuals appear able to transmit the virus to others. [ABSTRACT FROM AUTHOR]
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- 2020
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157. Contribution of Functional Antimalarial Immunity to Measures of Parasite Clearance in Therapeutic Efficacy Studies of Artemisinin Derivatives.
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O'Flaherty, Katherine, Ataíde, Ricardo, Zaloumis, Sophie G, Ashley, Elizabeth A, Powell, Rosanna, Feng, Gaoqian, Reiling, Linda, Dondorp, Arjen M, Day, Nicholas P, Dhorda, Mehul, Fairhurst, Rick M, Lim, Pharath, Amaratunga, Chanaki, Pukrittayakamee, Sasithon, Hien, Tran Tinh, Htut, Ye, Mayxay, Mayfong, Faiz, M Abul, Beeson, James G, and Nosten, Francois
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ARTEMISININ derivatives ,IMMUNOGLOBULIN G ,IMMUNITY ,PLASMODIUM ,PHAGOCYTOSIS - Abstract
Background: Antibodies to the blood stages of malaria parasites enhance parasite clearance and antimalarial efficacy. The antibody subclass and functions that contribute to parasite clearance during antimalarial treatment and their relationship to malaria transmission intensity have not been characterized.Methods: Levels of immunoglobulin G (IgG) subclasses and C1q fixation in response to Plasmodium falciparum merozoite antigens (erythrocyte-binding antigen [EBA] 175RIII-V, merozoite surface protein 2 [MSP-2], and MSP-142) and opsonic phagocytosis of merozoites were measured in a multinational trial assessing the efficacy of artesunate therapy across 11 Southeast Asian sites. Regression analyses assessed the effects of antibody seropositivity on the parasite clearance half-life (PC½), having a PC½ of ≥5 hours, and having parasitemia 3 days after treatment.Results: IgG3, followed by IgG1, was the predominant IgG subclass detected (seroprevalence range, 5%-35% for IgG1 and 27%-41% for IgG3), varied across study sites, and was lowest in study sites with the lowest transmission intensity and slowest mean PC½. IgG3, C1q fixation, and opsonic-phagocytosis seropositivity were associated with a faster PC½ (range of the mean reduction in PC½, 0.47-1.16 hours; P range, .001-.03) and a reduced odds of having a PC½ of ≥5 hours and having parasitemia 3 days after treatment.Conclusions: The prevalence of IgG3, complement-fixing antibodies, and merozoite phagocytosis vary according to transmission intensity, are associated with faster parasite clearance, and may be sensitive surrogates of an augmented clearance capacity of infected erythrocytes. Determining the functional immune mechanisms associated with parasite clearance will improve characterization of artemisinin resistance. [ABSTRACT FROM AUTHOR]- Published
- 2019
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158. A Pilot Study to Assess Safety and Feasibility of Intrathecal Immunoglobulin for the Treatment of Adults with Tetanus
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Loan, Huynh Thi, primary, Yen, Lam Minh, additional, Kestelyn, Evelyne, additional, Hao, Nguyen Van, additional, Mai, Nguyen Thi Hoang, additional, Thuy, Duong Bich, additional, Duong, Ha Thi Hai, additional, Dung, Nguyen Thi Phuong, additional, Phu, Nguyen Hoan, additional, Lieu, Pham Thi, additional, Thanh, Tran Tan, additional, Geskus, Ronald, additional, van Doorn, H. Rogier, additional, Tan, Le Van, additional, Wyncoll, Duncan, additional, Day, Nicholas P. J., additional, Hien, Tran Tinh, additional, Thwaites, Guy E., additional, Chau, Nguyen Van Vinh, additional, and Thwaites, C. Louise, additional
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- 2018
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159. Adjunctive dexamethasone for the treatment of HIV-infected adults with tuberculous meningitis (ACT HIV): Study protocol for a randomised controlled trial
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Donovan, Joseph, primary, Phu, Nguyen Hoan, additional, Mai, Nguyen Thi Hoang, additional, Dung, Le Tien, additional, Imran, Darma, additional, Burhan, Erlina, additional, Ngoc, Lam Hong Bao, additional, Bang, Nguyen Duc, additional, Giang, Do Chau, additional, Ha, Dang Thi Minh, additional, Day, Jeremy, additional, Thao, Le Thi Phuong, additional, Thuong, Nguyen TT, additional, Vien, Nguyen Nang, additional, Geskus, Ronald B., additional, Wolbers, Marcel, additional, Hamers, Raph L, additional, van Crevel, Reinout, additional, Nursaya, Mugi, additional, Maharani, Kartika, additional, Hien, Tran Tinh, additional, Baird, Kevin, additional, Lan, Nguyen Huu, additional, Kestelyn, Evelyne, additional, Chau, Nguyen Van Vinh, additional, and Thwaites, Guy E., additional
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- 2018
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160. Adjunctive dexamethasone for the treatment of HIV-uninfected adults with tuberculous meningitis stratified by Leukotriene A4 hydrolase genotype (LAST ACT): Study protocol for a randomised double blind placebo controlled non-inferiority trial
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Donovan, Joseph, primary, Phu, Nguyen Hoan, additional, Thao, Le Thi Phuong, additional, Lan, Nguyen Huu, additional, Mai, Nguyen Thi Hoang, additional, Trang, Nguyen Thi Mai, additional, Hiep, Nguyen Thi Thu, additional, Nhu, Tran Bao, additional, Hanh, Bui Thi Bich, additional, Mai, Vu Thi Phuong, additional, Bang, Nguyen Duc, additional, Giang, Do Chau, additional, Ha, Dang Thi Minh, additional, Day, Jeremy, additional, Thuong, Nguyen TT, additional, Vien, Nguyen Nang, additional, Geskus, Ronald B., additional, Hien, Tran Tinh, additional, Kestelyn, Evelyne, additional, Wolbers, Marcel, additional, Chau, Nguyen Van Vinh, additional, and Thwaites, Guy E., additional
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- 2018
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161. The prevalence, incidence and prevention of Plasmodium falciparum infections in forest rangers in Bu Gia Map National Park, Binh Phuoc province, Vietnam: a pilot study
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Son, Do Hung, primary, Thuy-Nhien, Nguyen, additional, von Seidlein, Lorenz, additional, Le Phuc-Nhi, Truong, additional, Phu, Ngo Thi, additional, Tuyen, Nguyen Thi Kim, additional, Tran, Nguyen Huyen, additional, Van Dung, Nguyen, additional, Van Quan, Bui, additional, Day, Nicholas P. J., additional, Dondorp, Arjen M., additional, White, Nicholas J., additional, Thwaites, Guy E., additional, and Hien, Tran Tinh, additional
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- 2017
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162. Early pandemic influenza (2009 H1N1) in Ho Chi Minh City, Vietnam: a clinical virological and epidemiological analysis
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Hien, Tran Tinh, Boni, Maciej F., Bryant, Juliet E., Ngan, Tran Thuy, Wolbers, Marcel, Nguyen, Tran Dang, Truong, Nguyen Thanh, Dung, Nguyen Thi, Ha, Do Quang, Hien, Vo Minh, Thanh, Tran Tan, Nhu, Le Nguyen Truc, Uyen, Le Thi Tam, Nhien, Pham Thi, Chinh, Nguyen Tran, Chau, Nguyen Van Vinh, Farrar, Jeremy, and van Doorn, H. Rogier
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Influenza -- Development and progression -- Risk factors -- Diagnosis -- Control -- Health aspects ,Ho Chi Minh City, Vietnam -- Health aspects - Abstract
Background: To date, little is known about the initial spread and response to the 2009 pandemic of novel influenza A ('2009 H1N1') in tropical countries. Here, we analyse the early progression of the epidemic from 26 May 2009 until the establishment of community transmission in the second half of July 2009 in Ho Chi Minh City (HCMC), Vietnam. In addition, we present detailed systematic viral clearance data on 292 isolated and treated patients and the first three cases of selection of resistant virus during treatment in Vietnam. Methods and Findings: Data sources included all available health reports from the Ministry of Health and relevant health authorities as well as clinical and laboratory data from the first confirmed cases isolated at the Hospital for Tropical Diseases in HCMC. Extensive reverse transcription (RT)-PCR diagnostics on serial samples, viral culture, neuraminidase-inhibition testing, and sequencing were performed on a subset of 2009 H1N1 confirmed cases. Virological (PCR status, shedding) and epidemiological (incidence, isolation, discharge) data were combined to reconstruct the initial outbreak and the establishment of community transmission. From 27 April to 24 July 2009, approximately 760,000 passengers who entered HCMC on international flights were screened at the airport by a body temperature scan and symptom questionnaire. Approximately 0.15% of incoming passengers were intercepted, 200 of whom tested positive for 2009 H1N1 by RT-PCR. An additional 121 out of 169 nontravelers tested positive after self-reporting or contact tracing. These 321 patients spent 79% of their PCR-positive days in isolation;60% of PCR-positive days were spent treated and in isolation. Influenza-like illness was noted in 61% of patients and no patients experienced pneumonia or severe outcomes. Viral clearance times were similar among patient groups with differing time intervals from illness onset to treatment, with estimated median clearance times between 2.6 and 2.8 d post-treatment for illness-to-treatment intervals of 1-4 d, and 2.0 d (95% confidence interval 1.5-2.5) when treatment was started on the first day of illness. Conclusions: The patients described here represent a cross-section of infected individuals that were identified by temperature screening and symptom questionnaires at the airport, as well as mildly symptomatic to moderately ill patients who self-reported to hospitals. Data are observational and, although they are suggestive, it is not possible to be certain whether the containment efforts delayed community transmission in Vietnam. Viral clearance data assessed by RT-PCR showed a rapid therapeutic response to oseltamivir. Please see later in the article for the Editors' Summary., Introduction Vietnam reported its first case of infection with 2009 pandemic influenza virus A (H1N1) on 31 May 2009, in a Vietnamese student returning from Wisconsin (United States) who had [...]
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- 2010
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163. Optimizing a multiplex high resolution melting curve to diagnose G6PD deficiency based on viangchan and canton mutations
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Hue Thi Nguyen, Hien Tran Tinh, Giang Thanh Nguyen-Dien, Anh Thi Lan Dang, Nghia Le Tri, and Ngoc Tran Bao
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0301 basic medicine ,Genetics ,education.field_of_study ,Enzyme disorder ,Population ,Biology ,General Biochemistry, Genetics and Molecular Biology ,High Resolution Melt ,03 medical and health sciences ,030104 developmental biology ,G6pd gene ,Mutation (genetic algorithm) ,Multiplex ,education ,Genotyping ,Single mutation - Abstract
Glucose-6-phosphate dehydrogenase (G6PD) deficiency which is caused by mutation on G6PD gene is the most common enzyme disorder in human. There have been 184 discovered mutations among which Viangchan [Val291Met] and Canton mutation [Arg459Leu] are the most common variants in Vietnamese. Due to the severity of this disease, several methods have been devised for diagnostics. However, time-consuming, low sensitivity and expensiveness are major problems of those techniques. Recently, High Resolution Melting (HRM) has been developed and proven to be an effective method for DNA genotyping, mutation scanning and sequence matching. Hence, in this study a multiplex HRM has been developed aiming at detecting these two mutations concurrently. At first, a singleplex HRM was designed for each mutation. Then, conditions for these singleplex assay were combined and optimized again in order to get the optimal condition for multiplex HRM. This method showed a promising potential with a high accuracy, sensitivity, and specificity, it is impractial to continue developing this method because of the lack of controls. The optimized singleplex PCR-HRM in this study still can be used for single mutation detection and serve as the background to develop PCR-HRM for other G6PD mutations in Vietnamese-Kinh population.
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- 2016
164. Clinical features of dengue in a large Vietnamese cohort: intrinsically lower platelet counts and greater risk for bleeding in adults than children
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Trung Dinh The, Thao Le Thi Thu, Dung Nguyen Minh, Ngoc Tran Van, Hien Tran Tinh, Chau Nguyen Van Vinh, Marcel Wolbers, Tam Dong Thi Hoai, Jeremy Farrar, Cameron Simmons, and Bridget Wills
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Adult ,Male ,Pediatrics ,medicine.medical_specialty ,Viral Diseases ,lcsh:Arctic medicine. Tropical medicine ,Adolescent ,lcsh:RC955-962 ,Secondary infection ,030231 tropical medicine ,Force of infection ,Hemorrhage ,Disease ,Risk Assessment ,Dengue fever ,Dengue Fever ,Dengue ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,Medicine ,Humans ,030212 general & internal medicine ,Prospective Studies ,Young adult ,Prospective cohort study ,Child ,Viral Hemorrhagic Fevers ,business.industry ,lcsh:Public aspects of medicine ,Public Health, Environmental and Occupational Health ,Age Factors ,Infant ,lcsh:RA1-1270 ,medicine.disease ,Thrombocytopenia ,Hospitals ,3. Good health ,Infectious Diseases ,Vietnam ,Arboviral Infections ,Child, Preschool ,Cohort ,Female ,business ,Risk assessment ,Research Article ,Neglected Tropical Diseases - Abstract
Background As dengue spreads to new geographical regions and the force of infection changes in existing endemic areas, a greater breadth of clinical presentations is being recognised. Clinical experience suggests that adults manifest a pattern of complications different from those observed in children, but few reports have described the age-related spectrum of disease in contemporaneous groups of patients recruited at the same geographical location. Methodology/Principal Findings Using detailed prospectively collected information from ongoing studies that encompass the full spectrum of hospitalised dengue cases admitted to a single hospital in southern Vietnam, we compared clinical and laboratory features, management, and outcome for 647 adults and 881 children with confirmed dengue. Signs of vascular leakage and shock were more frequent and more severe in children than adults, while bleeding manifestations and organ involvement were more common in adults. Additionally, adults experienced significantly more severe thrombocytopenia. Secondary infection but not serotype was independently associated with greater thrombocytopenia, although with a smaller effect than age-group. The effect of age-group on platelet count was also apparent in the values obtained several weeks after recovery, indicating that healthy adults have intrinsically lower counts compared to children. Conclusions/Significance There are clear distinctions between adults and children in the pattern of complications seen in association with dengue infection, and these depend partly on intrinsic age-dependent physiological differences. Knowledge of such differences is important to inform research on disease pathogenesis, as well as to encourage development of management guidelines that are appropriate to the age-groups at risk., Author Summary Dengue is a common and potentially serious viral illness. Complications include plasma leakage from small blood vessels causing shock and dysfunction of the systems that control blood clotting, resulting in bleeding. The disease used to affect children predominantly, but in recent years, the number of adult patients has been increasing. As there is limited data describing the patterns of complications by age, we performed this study to compare clinical and laboratory features, management, and outcomes of the disease for over 1,500 children and adults with confirmed dengue recruited at the same time at a single hospital in the Southern Vietnam. We found that plasma leakage and shock were more common and severe in children than adults, while bleeding and organ dysfunction were more frequent in adults. Adults had lower platelet counts throughout the illness course as well as at a follow-up visit several weeks after recovery. Platelets are a crucial element in controlling bleeding, and the intrinsically lower counts in adults compared to children may contribute to the greater risk for bleeding in this patient group. Knowledge about differences in the patterns of dengue-related complications between children and adults should help clinicians to diagnose and treat patients more effectively.
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- 2016
165. Genomic epidemiology of artemisinin resistant malaria
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Amato, Roberto, Miotto, Olivo, Woodrow, Charles J, Almagro-Garcia, Jacob, Sinha, Ipsita, Campino, Susana, Mead, Daniel, Drury, Eleanor, Kekre, Mihir, Sanders, Mandy, Amambua-Ngwa, Alfred, Amaratunga, Chanaki, Amenga-Etego, Lucas, Andrianaranjaka, Voahangy, Apinjoh, Tobias, Ashley, Elizabeth, Auburn, Sarah, Awandare, Gordon, Baraka, Vito, Barry, Alyssa, Boni, Maciej, Borrmann, Steffen, Bousema, Teun, Branch, Oralee, Bull, Peter, Chotivanich, Kesinee, Conway, David, Craig, Alister, Day, Nicholas, Djimde, Abdoulaye, Dolecek, Christiane, Dondorp, Arjen M, Drakeley, Chris, Duffy, Patrick, Echeverry, Diego F, Egwang, Thomas, Fairhurst, Rick, Faiz, Abul, Fanello, Caterina, Hien, Tran Tinh, Hodgson, Abraham, Imwong, Mallika, Ishengoma, Deus, Lim, Pharath, Lon, Chanthap, Marfurt, Jutta, Marsh, Kevin, Mayxay, Mayfong, Michon, Pascal, Mobegi, Victor, Mokuolu, Olugbenga, Montgomery, Jacqui, Mueller, Ivo, Kyaw, Myat Phone, Newton, Paul N, Nosten, François, Noviyanti, Rintis, Nzila, Alexis, Ocholla, Harold, Oduro, Abraham, Onyamboko, Marie, Ouédraogo, Jean-Bosco, Phyo, Aung, Plowe, Christopher, Price, Ric, Pukrittayakamee, Sasithon, Randrianarivelojosia, Milijaona, Ringwald, Pascal, Ruiz, Lastenia, Saunders, David, Shayo, Alex, Siba, Peter, Takala-Harrison, Shannon, Thanh, Thuy-Nhien, Thathy, Vandana, Verra, Federica, Wendler, Jason, White, Nicholas, Ye, Htut, Cornelius, Victoria, Giacomantonio, Rachel, Muddyman, Dawn, Henrichs, Christa, Malangone, Cinzia, Jyothi, Dushyanth, Pearson, Richard, Rayner, Julian, McVean, Gilean, Rockett, Kirk, Miles, Alistair, Vauterin, Paul, Jeffery, Ben, Manske, Magnus, Stalker, Jim, MacInnis, Bronwyn, Kwiatkowski, Dominic, The Wellcome Trust Sanger Institute [Cambridge], Mahidol Oxford Tropical Medicine Research Unit, University of Oxford [Oxford]-Mahidol University [Bangkok], The Wellcome Trust Centre for Human Genetics [Oxford], University of Oxford [Oxford], Medical Research Council Unit The Gambia (MRC), National Institute of Allergy and Infectious Diseases [Bethesda] (NIAID-NIH), National Institutes of Health [Bethesda] (NIH), Navrongo Health Research Centre [Navrongo, Ghana] (NHRC), G4 malaria group [Antananarivo, Madagascar] (IPM), Institut Pasteur de Madagascar, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), University of Buéa, Global and Tropical Health Division [Rocklands, Australia], Menzies School of Health Research [Australia], Charles Darwin University-Charles Darwin University, West African Centre for Cell Biology of Infectious Pathogens [Legon, Ghana] (WACCBIP), University of Ghana, National Institute for Medical Research [Tanzania] (NIMR), The Walter and Eliza Hall Institute of Medical Research (WEHI), Oxford University Clinical Research Unit [Ho Chi Minh City] (OUCRU), KEMRI-Wellcome Trust Research Programme (KWTRP), London School of Hygiene and Tropical Medicine (LSHTM), New York University School of Medicine, NYU System (NYU), Faculty of Tropical Medicine [Bangkok, Thailand], Mahidol University [Bangkok], Liverpool School of Tropical Medicine (LSTM), Faculté de Médecine, de pharmacie et d’Odonto-Stomatologie [Bamako, Mali] (FMPOS), Université de Bamako, Department of Entomology [West Lafayette], Purdue University [West Lafayette], Med Biotech Laboratories [Kampala, Ouganda] (MBL), Armed Forces Research Institute of Medical Sciences [Bangkok] (AFRIMS), Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Mahidol University [Bangkok]-Mahosot Hospital, Faculty of Medicine and Health Sciences [Madang, Papouasie-Nouvelle-Guinée], Divine Word University [Papouasie-Nouvelle-Guinée] (DWU), University of Ilorin, Department of Entomology [University Park], Pennsylvania State University (Penn State), Penn State System-Penn State System, University of Melbourne, Ministry of health and sport [Yangon, Myanmar] (MoHS), Eijkman Institute for Molecular Biology [Jakarta], King Fahd University of Petroleum and Minerals (KFUPM), Malawi Liverpool Wellcome Trust Clinical Research Programme (MLW), Liverpool School of Tropical Medicine (LSTM)-University of Liverpool-Wellcome Trust-University of Malawi, University of Kinshasa (UNIKIN), Institut de Recherche en Sciences de la Santé (IRSS) / Centre Muraz, Shoklo Malaria Research Unit [Mae Sot, Thailand] (SMRU), Mahidol Oxford Tropical Medicine Research Unit (MORU), Wellcome Trust-Mahidol University [Bangkok]-University of Oxford [Oxford]-Wellcome Trust-Mahidol University [Bangkok]-University of Oxford [Oxford], University of Maryland School of Medicine, University of Maryland System, Centre for Tropical Medicine and Global Health [Oxford, UK], Nuffield Department of Medicine [Oxford, UK] (Big Data Institute), University of Oxford [Oxford]-University of Oxford [Oxford], Department of Clinical Tropical Medicine [Bangkok, Thailand] (Faculty of Tropical Medicine), Global Malaria Programme, Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Universidad Nacional de la Amazonía Peruana [Loreto, Perou] (UNAP), University of Dodoma [Tanzanie] (UDOM), Papua New Guinea Institute of Medical Research (PNG-IMR), Wellcome Trust-Mahidol University [Bangkok]-University of Oxford [Oxford], The sequencing, analysis, informatics and management of the Community Project are supported by the Wellcome Trust through Sanger Institute core funding (098051) and a Strategic Award (090770/Z/09/Z) and by the MRC Centre for Genomics and Global Health which is jointly funded by the Medical Research Council and the Department for International Development (DFID) (G0600718, M006212). AEB and IM acknowledge the Victorian State Government Operational Infrastructure Support and Australian Government NHMRC IRIISS., This study was conducted by the MalariaGEN Plasmodium falciparum Community Project, and was made possible by clinical parasite samples contributed by partner studies, whose investigators are represented in the author list. RA and OM contributed equally. In addition, the authors would like to thank the following individuals, who contributed to partner studies or to the MalariaGEN Resource Centre, making this study possible: James Abugri, Nicholas Amoako, Steven M Kiara, John Okombo, Rogelin Raherinjafy, Seheno Razanatsiorimalala, Hongying Jiang, Xin-zhuan Su., and MalariaGEN Plasmodium Falciparum C
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0301 basic medicine ,Drug resistance ,Pharmacology ,MESH: Asia, Southeastern/epidemiology ,MESH: Malaria, Falciparum/epidemiology ,Malaria, Falciparum ,Artemisinin ,Biology (General) ,GeneralLiterature_REFERENCE(e.g.,dictionaries,encyclopedias,glossaries) ,Asia, Southeastern ,Genetics ,Molecular Epidemiology ,Microbiology and Infectious Disease ,education.field_of_study ,MESH: Plasmodium falciparum/genetics ,General Neuroscience ,General Medicine ,MESH: Artemisinins/pharmacology ,Artemisinins ,3. Good health ,Genomics and Evolutionary Biology ,kelch13 ,Medicine ,MESH: Plasmodium falciparum/drug effects ,Research Article ,medicine.drug ,Combination therapy ,plasmodium falciparum ,QH301-705.5 ,infectious disease ,MESH: Antimalarials/pharmacology ,Science ,030106 microbiology ,Population ,malaria ,MESH: Plasmodium falciparum/classification ,MESH: Malaria, Falciparum/parasitology ,Biology ,General Biochemistry, Genetics and Molecular Biology ,Antimalarials ,03 medical and health sciences ,parasitic diseases ,medicine ,genomics ,MESH: Molecular Epidemiology ,education ,MESH: Drug Resistance ,[SDV.GEN]Life Sciences [q-bio]/Genetics ,drug resistance ,General Immunology and Microbiology ,Molecular epidemiology ,evolutionary biology ,microbiology ,Plasmodium falciparum ,medicine.disease ,biology.organism_classification ,MESH: Africa/epidemiology ,lnfectious Diseases and Global Health Radboud Institute for Health Sciences [Radboudumc 4] ,030104 developmental biology ,artemisinin ,Infectious disease (medical specialty) ,Africa ,Other ,Human medicine ,MESH: Plasmodium falciparum/isolation & purification ,Malaria - Abstract
The current epidemic of artemisinin resistant Plasmodium falciparum in Southeast Asia is the result of a soft selective sweep involving at least 20 independent kelch13 mutations. In a large global survey, we find that kelch13 mutations which cause resistance in Southeast Asia are present at low frequency in Africa. We show that African kelch13 mutations have originated locally, and that kelch13 shows a normal variation pattern relative to other genes in Africa, whereas in Southeast Asia there is a great excess of non-synonymous mutations, many of which cause radical amino-acid changes. Thus, kelch13 is not currently undergoing strong selection in Africa, despite a deep reservoir of variations that could potentially allow resistance to emerge rapidly. The practical implications are that public health surveillance for artemisinin resistance should not rely on kelch13 data alone, and interventions to prevent resistance must account for local evolutionary conditions, shown by genomic epidemiology to differ greatly between geographical regions. DOI: http://dx.doi.org/10.7554/eLife.08714.001, eLife digest Malaria is an infectious disease caused by a microscopic parasite called Plasmodium, which is transferred between humans by mosquitos. One species of malaria parasite called Plasmodium falciparum can cause particularly severe and life-threatening forms of the disease. Currently, the most widely used treatment for P. falciparum infections is artemisinin combination therapy, a treatment that combines the drug artemisinin (or a closely related molecule) with another antimalarial drug. However, resistance to artemisinin has started to spread throughout Southeast Asia. Artemisinin resistance is caused by mutations in a parasite gene called kelch13, and researchers have identified over 20 different mutations in P. falciparum that confer artemisinin resistance. The diversity of mutations involved, and the fact that the same mutation can arise independently in different locations, make it difficult to track the spread of resistance using conventional molecular marker approaches. Here, Amato, Miotto et al. sequenced the entire genomes of more than 3,000 clinical samples of P. falciparum from Southeast Asia and Africa, collected as part of a global network of research groups called the MalariaGEN Plasmodium falciparum Community Project. Amato, Miotto et al. found that African parasites had independently acquired many of the same kelch13 mutations that are known to cause resistance to artemisinin in Southeast Asia. However the kelch13 mutations seen in Africa remained at low levels in the parasite population, and appeared to be under much less pressure for evolutionary selection than those found in Southeast Asia. These findings demonstrate that the emergence and spread of resistance to antimalarial drugs does not depend solely on the mutational process, but also on other factors that influence whether the mutations will spread in the population. Understanding how this is affected by different patterns of drug treatments and other environmental conditions will be important in developing more effective strategies for combating malaria. DOI: http://dx.doi.org/10.7554/eLife.08714.002
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- 2016
166. High-performance liquid chromatography with time-programmed fluorescence detection for the quantification of Levofloxacin in human plasma and cerebrospinal fluid in adults with tuberculous meningitis
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Van Toi, Pham, primary, Pouplin, Thomas, additional, Tho, Nguyen Duc Khanh, additional, Phuong, Pham Nguyen, additional, Chau, Tran Thi Hong, additional, Thuong Thuong, Nguyen Thuy, additional, Heemskerk, Dorothee, additional, Hien, Tran Tinh, additional, and Thwaites, Guy E., additional
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- 2017
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167. The decline of malaria in Vietnam, 1991-2014
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Goldlust, Sandra M, primary, Thuan, Phung Duc, additional, Duy Hoang Giang, Dang, additional, Thang, Ngo Duc, additional, Thwaites, Guy E, additional, Farrar, Jeremy, additional, Thanh, Ngo Viet, additional, Nguyen, Tran Dang, additional, Grenfell, Bryan T, additional, Boni, Maciej F, additional, and Hien, Tran Tinh, additional
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- 2017
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168. K13 Propeller Mutations in Plasmodium falciparum Populations in Regions of Malaria Endemicity in Vietnam from 2009 to 2016
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Thuy-Nhien, Nguyen, primary, Tuyen, Nguyen Kim, additional, Tong, Nguyen Thanh, additional, Vy, Nguyen Tuong, additional, Thanh, Ngo Viet, additional, Van, Huynh Thuy, additional, Huong-Thu, Pham, additional, Quang, Huynh Hong, additional, Boni, Maciej F., additional, Dolecek, Christiane, additional, Farrar, Jeremy, additional, Thwaites, Guy E., additional, Miotto, Olivo, additional, White, Nicholas J., additional, and Hien, Tran Tinh, additional
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- 2017
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169. Estimation of the In Vivo MIC of Cipargamin in Uncomplicated Plasmodium falciparum Malaria
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Hien, Tran Tinh, primary, White, Nicholas J., additional, Thuy-Nhien, Nguyen Thanh, additional, Hoa, Nhu Thi, additional, Thuan, Phung Duc, additional, Tarning, Joel, additional, Nosten, François, additional, Magnusson, Baldur, additional, Jain, Jay Prakash, additional, and Hamed, Kamal, additional
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- 2017
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170. Rapid decline in the susceptibility of Plasmodium falciparum to dihydroartemisinin–piperaquine in the south of Vietnam
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Thanh, Ngo Viet, primary, Thuy-Nhien, Nguyen, additional, Tuyen, Nguyen Thi Kim, additional, Tong, Nguyen Thanh, additional, Nha-Ca, Nguyen Thuy, additional, Dong, Le Thanh, additional, Quang, Huynh Hong, additional, Farrar, Jeremy, additional, Thwaites, Guy, additional, White, Nicholas J., additional, Wolbers, Marcel, additional, and Hien, Tran Tinh, additional
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- 2017
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171. Community perceptions of targeted anti-malarial mass drug administrations in two provinces in Vietnam: a quantitative survey
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Nguyen, Thuy-Nhien, primary, Thu, Pham N. Huong, additional, Hung, Ngo Trong, additional, Son, Do Hung, additional, Tien, Nguyen Thanh, additional, Van Dung, Nguyen, additional, Quang, Huynh Hong, additional, Seidlein, Lorenz von, additional, Cheah, Phaik Yeong, additional, Dondorp, Arjen M., additional, Day, Nicholas P. J., additional, White, Nicholas J., additional, and Hien, Tran Tinh, additional
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- 2017
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172. Defining Surrogate Endpoints for Clinical Trials in Severe Falciparum Malaria
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Jeeyapant, Atthanee, primary, Kingston, Hugh W., additional, Plewes, Katherine, additional, Maude, Richard J., additional, Hanson, Josh, additional, Herdman, M. Trent, additional, Leopold, Stije J., additional, Ngernseng, Thatsanun, additional, Charunwatthana, Prakaykaew, additional, Phu, Nguyen Hoan, additional, Ghose, Aniruddha, additional, Hasan, M. Mahtab Uddin, additional, Fanello, Caterina I., additional, Faiz, Md Abul, additional, Hien, Tran Tinh, additional, Day, Nicholas P. J., additional, White, Nicholas J., additional, and Dondorp, Arjen M., additional
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- 2017
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173. The impact of environmental and climatic variation on the spatiotemporal trends of hospitalized pediatric diarrhea in Ho Chi Minh City, Vietnam
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Ngoc Minh N.M. Nguyen, Jonathan L. Zelner, Tran Do Hoang Nhu, Emmiliese von Clemm, Stephen Baker, Tu Van Hoang Minh, Phuc Hoang Le, Hien Tran Tinh, Chau Nguyen Van Vinh, My V. T. Phan, Hung Nguyen Thanh, Bryan T. Grenfell, Vi Lu Lan, Harry Storch, Tuan Ha Manh, Guy E. Thwaites, Duong Vu Thuy, and Corinne N. Thompson
- Subjects
Diarrhea ,Male ,Pediatrics ,medicine.medical_specialty ,Health (social science) ,Geography, Planning and Development ,Psychological intervention ,Mixed effects regression ,Environment ,Article ,Health(social science) ,Environmental health ,parasitic diseases ,medicine ,Mixed effects ,Humans ,Climate change ,Child ,business.industry ,Public health ,Public Health, Environmental and Occupational Health ,Hospitals, Pediatric ,6. Clean water ,Ho chi minh ,3. Good health ,Vietnam ,13. Climate action ,Air temperature ,Female ,Seasons ,medicine.symptom ,Diarrheal disease ,business ,Spatial risk - Abstract
It is predicted that the integration of climate-based early warning systems into existing action plans will facilitate the timely provision of interventions to diarrheal disease epidemics in resource-poor settings. Diarrhea remains a considerable public health problem in Ho Chi Minh City (HCMC), Vietnam and we aimed to quantify variation in the impact of environmental conditions on diarrheal disease risk across the city. Using all inpatient diarrheal admissions data from three large hospitals within HCMC, we developed a mixed effects regression model to differentiate district-level variation in risk due to environmental conditions from the overarching seasonality of diarrheal disease hospitalization in HCMC. We identified considerable spatial heterogeneity in the risk of all-cause diarrhea across districts of HCMC with low elevation and differential responses to flooding, air temperature, and humidity driving further spatial heterogeneity in diarrheal disease risk. The incorporation of these results into predictive forecasting algorithms will provide a powerful resource to aid diarrheal disease prevention and control practices in HCMC and other similar settings., Highlights • Mixed effect modeling of hospitalized diarrheal admissions. • Diarrhea is seasonal and spatially heterogeneous in Ho Chi Minh City, Vietnam. • Low elevation is a significant risk for pediatric diarrheal disease. • Spatial heterogeneity in risk is due to flooding and other environmental factors. • Climate based predictive modeling may help reduce diarrheal epidemics in the future.
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- 2015
174. An HPLC method with diode array detector for the simultaneous quantification of chloroquine and desethylchloroquine in plasma and whole blood samples from Plasmodium vivax patients in Vietnam, using quinine as an internal standard
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Toi, Van Pham, Phuong, Pham Nguyen, Tho, Nguyen Duc Khanh, Nhien, Nguyen Thanh Thuy, Ca, Nguyen Thuy Nha, Thomas, Pouplin, Jeremy, Farrar, Guy E, Thwaites, and Hien, Tran Tinh
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malaria ,Reproducibility of Results ,Chloroquine ,Reference Standards ,desethylchloroquine ,Vietnam ,HPLC‐DAD ,Limit of Detection ,Malaria, Vivax ,Humans ,Spectrophotometry, Ultraviolet ,SPE ,Plasmodium vivax ,Chromatography, High Pressure Liquid ,Research Articles ,Research Article - Abstract
A sensitive, simple method for quantification of chloroquine (CQ) and desethylchloroquine (MCQ) in whole blood and plasma from Plasmodium vivax patients has been developed using HPLC with diode array detection (DAD). Solid‐phase extraction on Isolute‐96‐CBA was employed to process 100 μL of plasma/whole blood samples. CQ, MCQ and quinine were separated using a mobile phase of phosphate buffer 25 mm, pH 2.60–acetonitrile (88:12, v/v) with 2 mm sodium perchlorate on a Zorbax SB‐CN 150 × 4.6 mm, 5 μm column at a flow rate of 1.2 mL/min, at ambient temperature in 10 min, with the DAD wavelength of 343 nm. The method was linear over the range of 10–5000 ng/mL for both CQ and MCQ in plasma and whole blood. The limit of detection was 4 ng/mL and limit of quantification was 10 ng/mL in both plasma and blood for CQ and MCQ. The intra‐, inter‐ and total assay precision were
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- 2015
175. Erratum for Parry et al., Clinically and Microbiologically Derived Azithromycin Susceptibility Breakpoints for Salmonella enterica Serovars Typhi and Paratyphi A
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Arjen M. Dondorp, Nicholas P. J. Day, James Campbell, Monorama Deb, Vinh Ha, Jeremy Farrar, Chinh Nguyen Tran, Guy E. Thwaites, Christiane Dolecek, Abhilasha Karkey, Rang Nguyen Ngoc, Sach Nguyen Van, Paul N. Newton, Stephen Baker, Tuyen Ha Thanh, Sabina Dongol, Nga Tran Vu Thieu, Christopher M. Parry, Rajni Gaind, Ho Van Anh, La Tran Thi Phi, Ruchi Gupta, Buddha Basnyat, Phuong Le Thi, Paul Turner, Soeng Sona, Rattanaphone Phetsouvanh, Aniruddha Ghose, Duy Pham Thanh, Mohamed Abul Faiz, Hien Tran Tinh, Catrin E. Moore, David A. B. Dance, Rapeephan R. Maude, and Bay Pham Van Be
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Serotype ,Male ,Adolescent ,Microbial Sensitivity Tests ,Azithromycin ,Bioinformatics ,Serogroup ,Microbiology ,Young Adult ,Medicine ,Humans ,Pharmacology (medical) ,Typhoid Fever ,Child ,Pharmacology ,biology ,business.industry ,Breakpoint ,Salmonella enterica ,biology.organism_classification ,Infectious Diseases ,Female ,Erratum ,business ,medicine.drug - Abstract
Azithromycin is an effective treatment for uncomplicated infections with Salmonella enterica serovar Typhi and serovar Paratyphi A (enteric fever), but there are no clinically validated MIC and disk zone size interpretative guidelines. We studied individual patient data from three randomized controlled trials (RCTs) of antimicrobial treatment in enteric fever in Vietnam, with azithromycin used in one treatment arm, to determine the relationship between azithromycin treatment response and the azithromycin MIC of the infecting isolate. We additionally compared the azithromycin MIC and the disk susceptibility zone sizes of 1,640 S. Typhi and S. Paratyphi A clinical isolates collected from seven Asian countries. In the RCTs, 214 patients who were treated with azithromycin at a dose of 10 to 20 mg/ml for 5 to 7 days were analyzed. Treatment was successful in 195 of 214 (91%) patients, with no significant difference in response (cure rate, fever clearance time) with MICs ranging from 4 to 16 μg/ml. The proportion of Asian enteric fever isolates with an MIC of ≤ 16 μg/ml was 1,452/1,460 (99.5%; 95% confidence interval [CI], 98.9 to 99.7) for S. Typhi and 207/240 (86.3%; 95% CI, 81.2 to 90.3) (P0.001) for S. Paratyphi A. A zone size of ≥ 13 mm to a 5-μg azithromycin disk identified S. Typhi isolates with an MIC of ≤ 16 μg/ml with a sensitivity of 99.7%. An azithromycin MIC of ≤ 16 μg/ml or disk inhibition zone size of ≥ 13 mm enabled the detection of susceptible S. Typhi isolates that respond to azithromycin treatment. Further work is needed to define the response to treatment in S. Typhi isolates with an azithromycin MIC of16 μg/ml and to determine MIC and disk breakpoints for S. Paratyphi A.
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- 2015
176. Genomic epidemiology of the current wave of artemisinin resistant malaria
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Amato, Roberto, Miotto, Olivo, Woodrow, Charles, Almagro-Garcia, Jacob, Sinha, Ipsita, Campino, Susana, Mead, Daniel, Drury, Eleanor, Kekre, Mihir, Sanders, Mandy, Amambua-Ngwa, Alfred, Amaratunga, Chanaki, Amenga-Etego, Lucas, Anderson, Tim JC, Andrianaranjaka, Voahangy, Apinjoh, Tobias, Ashley, Elizabeth, Auburn, Sarah, Awandare, Gordon A, Baraka, Vito, Barry, Alyssa, Boni, Maciej F, Borrmann, Steffen, Bousema, Teun, Branch, Oralee, Bull, Peter C, Chotivanich, Kesinee, Conway, David J, Craig, Alister, Day, Nicholas P, Djimdé, Abdoulaye, Dolecek, Christiane, Dondorp, Arjen M, Drakeley, Chris, Duffy, Patrick, Echeverri-Garcia, Diego F, Egwang, Thomas G, Fairhurst, Rick M, Faiz, Md. Abul, Fanello, Caterina I, Hien, Tran Tinh, Hodgson, Abraham, Imwong, Mallika, Ishengoma, Deus, Lim, Pharath, Lon, Chanthap, Marfurt, Jutta, Marsh, Kevin, Mayxay, Mayfong, Mobegi, Victor, Mokuolu, Olugbenga, Montgomery, Jacqui, Mueller, Ivo, Kyaw, Myat Phone, Newton, Paul N, Nosten, Francois, Noviyanti, Rintis, Nzila, Alexis, Ocholla, Harold, Oduro, Abraham, Onyamboko, Marie, Ouedraogo, Jean-Bosco, Phyo, Aung Pyae, Plowe, Christopher V, Price, Ric N, Pukrittayakamee, Sasithon, Randrianarivelojosia, Milijaona, Ringwald, Pascal, Ruiz, Lastenia, Saunders, David, Shayo, Alex, Siba, Peter, Takala-Harrison, Shannon, Thanh, Thuy-Nhien Nguyen, Thathy, Vandana, Verra, Federica, White, Nicholas J, Htut, Ye, Cornelius, Victoria J, Giacomantonio, Rachel, Muddyman, Dawn, Henrichs, Christa, Malangone, Cinzia, Jyothi, Dushyanth, Pearson, Richard D, Rayner, Julian C, McVean, Gilean, Rockett, Kirk, Miles, Alistair, Vauterin, Paul, Jeffery, Ben, Manske, Magnus, Stalker, Jim, MacInnis, Bronwyn, and Kwiatkowski, Dominic P
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0303 health sciences ,medicine.medical_specialty ,biology ,Resistance (ecology) ,business.industry ,030231 tropical medicine ,Plasmodium falciparum ,medicine.disease ,biology.organism_classification ,3. Good health ,Biotechnology ,03 medical and health sciences ,0302 clinical medicine ,Public health surveillance ,Evolutionary biology ,parasitic diseases ,Epidemiology ,medicine ,Artemisinin ,Current wave ,Selective sweep ,business ,Malaria ,030304 developmental biology ,medicine.drug - Abstract
Artemisinin resistant Plasmodium falciparum is advancing across Southeast Asia in a soft selective sweep involving at least 20 independent kelch13 mutations. In a large global survey, we find that kelch13 mutations which cause resistance in Southeast Asia are present at low frequency in Africa. We show that African kelch13 mutations have originated locally, and that kelch13 shows a normal variation pattern relative to other genes in Africa, whereas in Southeast Asia there is a great excess of non‐synonymous mutations, many of which cause radical amino‐acid changes. Thus, kelch13 is not currently undergoing strong selection in Africa, despite a deep reservoir of standing variation that could potentially allow resistance to emerge rapidly. The practical implications are that public health surveillance for artemisinin resistance should not rely on kelch13 data alone, and interventions to prevent resistance must account for local evolutionary conditions, shown by genomic epidemiology to differ greatly between geographical regions.
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- 2015
177. Clinically and microbiologically derived azithromycin susceptibility breakpoints for Salmonella enterica serovars Typhi and Paratyphi A
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Paul N. Newton, Vinh Ha, Christopher M. Parry, James Campbell, Sach Nguyen Van, Catrin E. Moore, Christiane Dolecek, Ruchi Gupta, Sabina Dongol, Abhilasha Karkey, Nicholas P. J. Day, Buddha Basnyat, David A. B. Dance, Stephen Baker, Nga Tran Vu Thieu, Tuyen Ha Thanh, Bay Pham Van Be, Duy Pham Thanh, Guy E. Thwaites, La Tran Thi Phi, Paul Turner, Rajni Gaind, Phuong Le Thi, Aniruddha Ghose, Soeng Sona, Arjen M. Dondorp, Jeremy Farrar, Monorama Deb, Rang Nguyen Ngoc, Mohamed Abul Faiz, Chinh Nguyen Tran, Hien Tran Tinh, Rapeephan R. Maude, Ho Van Anh, and Rattanaphone Phetsouvanh
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Serotype ,medicine.drug_class ,030231 tropical medicine ,Antibiotics ,Drug resistance ,Azithromycin ,Typhoid fever ,Macrolide Antibiotics ,Microbiology ,03 medical and health sciences ,0302 clinical medicine ,Medicine ,Pharmacology (medical) ,Pharmacology ,0303 health sciences ,biology ,030306 microbiology ,business.industry ,biology.organism_classification ,medicine.disease ,Antimicrobial ,bacterial infections and mycoses ,3. Good health ,Infectious Diseases ,Salmonella enterica ,Susceptibility ,business ,medicine.drug - Abstract
Azithromycin is an effective treatment for uncomplicated infections with Salmonella enterica serovar Typhi and serovar Paratyphi A (enteric fever), but there are no clinically validated MIC and disk zone size interpretative guidelines. We studied individual patient data from three randomized controlled trials (RCTs) of antimicrobial treatment in enteric fever in Vietnam, with azithromycin used in one treatment arm, to determine the relationship between azithromycin treatment response and the azithromycin MIC of the infecting isolate. We additionally compared the azithromycin MIC and the disk susceptibility zone sizes of 1,640 S . Typhi and S . Paratyphi A clinical isolates collected from seven Asian countries. In the RCTs, 214 patients who were treated with azithromycin at a dose of 10 to 20 mg/ml for 5 to 7 days were analyzed. Treatment was successful in 195 of 214 (91%) patients, with no significant difference in response (cure rate, fever clearance time) with MICs ranging from 4 to 16 μg/ml. The proportion of Asian enteric fever isolates with an MIC of ≤16 μg/ml was 1,452/1,460 (99.5%; 95% confidence interval [CI], 98.9 to 99.7) for S . Typhi and 207/240 (86.3%; 95% CI, 81.2 to 90.3) ( P < 0.001) for S . Paratyphi A. A zone size of ≥13 mm to a 5-μg azithromycin disk identified S . Typhi isolates with an MIC of ≤16 μg/ml with a sensitivity of 99.7%. An azithromycin MIC of ≤16 μg/ml or disk inhibition zone size of ≥13 mm enabled the detection of susceptible S . Typhi isolates that respond to azithromycin treatment. Further work is needed to define the response to treatment in S . Typhi isolates with an azithromycin MIC of >16 μg/ml and to determine MIC and disk breakpoints for S . Paratyphi A.
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- 2015
178. Baseline data of parasite clearance in patients with falciparum malaria treated with an artemisinin derivative : an individual patient data meta-analysis
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WWARN Parasite Clearance Study Group, Abdulla, Salim, Ashley, Elizabeth A, Bassat, Quique, Bethell, Delia, Björkman, Anders, Borrmann, Steffen, D'Alessandro, Umberto, Dahal, Prabin, Day, Nicholas P, Diakite, Mahamadou, Djimde, Abdoulaye A, Dondorp, Arjen M, Duong, Socheat, Edstein, Michael D, Fairhurst, Rick M, Faiz, M Abul, Falade, Catherine, Flegg, Jennifer A, Fogg, Carole, Gonzalez, Raquel, Greenwood, Brian, Guérin, Philippe J, Guthmann, Jean-Paul, Hamed, Kamal, Hien, Tran Tinh, Htut, Ye, Juma, Elizabeth, Lim, Pharath, Mårtensson, Andreas, Mayxay, Mayfong, Mokuolu, Olugbenga A, Moreira, Clarissa, Newton, Paul, Noedl, Harald, Nosten, Francois, Ogutu, Bernhards R, Onyamboko, Marie A, Owusu-Agyei, Seth, Phyo, Aung Pyae, Premji, Zul, Price, Ric N, Pukrittayakamee, Sasithon, Ramharter, Michael, Sagara, Issaka, Se, Youry, Suon, Seila, Stepniewska, Kasia, Ward, Stephen A, White, Nicholas J, and Winstanley, Peter A
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Male ,Artemether/lumefantrine ,Social and Clinical Pharmacy ,Drug Resistance ,Physiology ,Parasitemia ,Drug resistance ,chemistry.chemical_compound ,Medicine ,Parasite hosting ,Artemisinin ,Malaria, Falciparum ,Child ,Diagnosis & treatment ,Clinical Trials as Topic ,Surveillance, monitoring, evaluation ,biology ,Public Health, Global Health, Social Medicine and Epidemiology ,Middle Aged ,Artemisinins ,3. Good health ,Infectious Diseases ,Blood ,Artemisinin resistance ,Child, Preschool ,Female ,medicine.drug ,Adult ,endocrine system ,Adolescent ,Plasmodium falciparum ,Antimalarials ,Young Adult ,Health Sciences ,parasitic diseases ,Animals ,Humans ,Aged ,Parasite clearance ,business.industry ,Research ,Samhällsfarmaci och klinisk farmaci ,Infant ,medicine.disease ,biology.organism_classification ,Malaria ,Folkhälsovetenskap, global hälsa, socialmedicin och epidemiologi ,chemistry ,Artesunate ,Immunology ,Parasitology ,business - Abstract
Background Artemisinin resistance in Plasmodium falciparum manifests as slow parasite clearance but this measure is also influenced by host immunity, initial parasite biomass and partner drug efficacy. This study collated data from clinical trials of artemisinin derivatives in falciparum malaria with frequent parasite counts to provide reference parasite clearance estimates stratified by location, treatment and time, to examine host factors affecting parasite clearance, and to assess the relationships between parasite clearance and risk of recrudescence during follow-up. Methods Data from 24 studies, conducted from 1996 to 2013, with frequent parasite counts were pooled. Parasite clearance half-life (PC1/2) was estimated using the WWARN Parasite Clearance Estimator. Random effects regression models accounting for study and site heterogeneity were used to explore factors affecting PC1/2 and risk of recrudescence within areas with reported delayed parasite clearance (western Cambodia, western Thailand after 2000, southern Vietnam, southern Myanmar) and in all other areas where parasite populations are artemisinin sensitive. Results PC1/2 was estimated in 6975 patients, 3288 of whom also had treatment outcomes evaluate d during 28–63 days follow-up, with 93 (2.8 %) PCR-confirmed recrudescences. In areas with artemisinin-sensitive parasites, the median PC1/2 following three-day artesunate treatment (4 mg/kg/day) ranged from 1.8 to 3.0 h and the proportion of patients with PC1/2 >5 h from 0 to 10 %. Artesunate doses of 4 mg/kg/day decreased PC1/2 by 8.1 % (95 % CI 3.2–12.6) compared to 2 mg/kg/day, except in populations with delayed parasite clearance. PC1/2 was longer in children and in patients with fever or anaemia at enrolment. Long PC1/2 (HR = 2.91, 95 % CI 1.95–4.34 for twofold increase, p
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- 2015
179. A Randomized Comparison of Chloroquine Versus Dihydroartemisinin–Piperaquine for the Treatment of Plasmodium vivax Infection in Vietnam
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Thuan, Phung Duc, primary, Ca, Nguyen Thuy Nha, additional, Van Toi, Pham, additional, Nhien, Nguyen Thanh Thuy, additional, Thanh, Ngo Viet, additional, Anh, Nguyen Duc, additional, Phu, Nguyen Hoan, additional, Thai, Cao Quang, additional, Thai, Le Hong, additional, Hoa, Nhu Thi, additional, Dong, Le Thanh, additional, Loi, Mai Anh, additional, Son, Do Hung, additional, Khanh, Tran Tinh Ngoc, additional, Dolecek, Christiane, additional, Nhan, Ho Thi, additional, Wolbers, Marcel, additional, Thwaites, Guy, additional, Farrar, Jeremy, additional, White, Nicholas J., additional, and Hien, Tran Tinh, additional
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- 2016
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180. Population pharmacokinetic properties of artemisinin in healthy male Vietnamese volunteers
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Birgersson, Sofia, primary, Van Toi, Pham, additional, Truong, Nguyen Thanh, additional, Dung, Nguyen Thi, additional, Ashton, Michael, additional, Hien, Tran Tinh, additional, Abelö, Angela, additional, and Tarning, Joel, additional
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- 2016
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181. In vitro activity of colistin in antimicrobial combination against carbapenem-resistant Acinetobacter baumannii isolated from patients with ventilator-associated pneumonia in Vietnam
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Le Minh, Vien, primary, Thi Khanh Nhu, Nguyen, additional, Vinh Phat, Voong, additional, Thompson, Corinne, additional, Huong Lan, Nguyen Phu, additional, Thieu Nga, Tran Vu, additional, Thanh Tam, Pham Thi, additional, Tuyen, Ha Thanh, additional, Hoang Nhu, Tran Do, additional, Van Hao, Nguyen, additional, Thi Loan, Huynh, additional, Minh Yen, Lam, additional, Parry, Christopher M., additional, Trung Nghia, Ho Dang, additional, Campbell, James I., additional, Hien, Tran Tinh, additional, Thwaites, Louise, additional, Thwaites, Guy, additional, Van Vinh Chau, Nguyen, additional, and Baker, Stephen, additional
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- 2015
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182. Human SNP links differential outcomes in inflammatory and infectious disease to a FOXO3-regulated pathway
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Lee, James C, Espéli, Marion, Anderson, Carl A, Linterman, Michelle A, Pocock, Joanna M, Williams, Naomi J, Roberts, Rebecca, Viatte, Sebastien, Fu, Bo, Peshu, Norbert, Hien, Tran Tinh, Phu, Nguyen Hoan, Wesley, Emma, Edwards, Cathryn, Ahmad, Tariq, Mansfield, John C, Gearry, Richard, Dunstan, Sarah, Williams, Thomas N, Barton, Anne, Vinuesa, Carola G, Drummond, Hazel, Kennedy, Nick, Lees, Charlie, Satsangi, Jack, Parkes, Miles, Lyons, Paul A, Smith, Kenneth G C, and Henderson, Paul
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Cell Nucleus ,Inflammation ,Extracellular Matrix Proteins ,Transcription, Genetic ,Genetic Variation ,Forkhead Transcription Factors ,Polymorphism, Single Nucleotide ,Monocytes ,Arthritis, Rheumatoid ,Mice ,Crohn Disease ,Transforming Growth Factor beta ,Animals ,Humans ,Malaria, Falciparum - Abstract
The clinical course and eventual outcome, or prognosis, of complex diseases varies enormously between affected individuals. This variability critically determines the impact a disease has on a patient's life but is very poorly understood. Here, we exploit existing genome-wide association study data to gain insight into the role of genetics in prognosis. We identify a noncoding polymorphism in FOXO3A (rs12212067: T > G) at which the minor (G) allele, despite not being associated with disease susceptibility, is associated with a milder course of Crohn's disease and rheumatoid arthritis and with increased risk of severe malaria. Minor allele carriage is shown to limit inflammatory responses in monocytes via a FOXO3-driven pathway, which through TGFβ1 reduces production of proinflammatory cytokines, including TNFα, and increases production of anti-inflammatory cytokines, including IL-10. Thus, we uncover a shared genetic contribution to prognosis in distinct diseases that operates via a FOXO3-driven pathway modulating inflammatory responses.
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- 2013
183. Sharing experiences: towards an evidence based model of dengue surveillance and outbreak response in Latin America and Asia
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Ronald Skewes, Jeremy Farrar, Axel Kroeger, Silvia Runge Ranzinger, Hien Tran Tinh, Novia Kuswara, Ernesto Gozzer, Priscila Leite, Yodi Mahendradhata, Ayesha J Verrall, David Benitez Valladares, and Shiraz Badurdeen
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Veterinary medicine ,medicine.medical_specialty ,Asia ,Latin Americans ,wa_950 ,030231 tropical medicine ,wa_395 ,Dengue virus ,medicine.disease_cause ,Risk Assessment ,wa_110 ,Disease Outbreaks ,Dengue fever ,Dengue ,03 medical and health sciences ,0302 clinical medicine ,Outbreak detection ,Environmental health ,wc_528 ,medicine ,Humans ,030212 general & internal medicine ,Outbreak response ,Disease surveillance ,Contingency plan ,Evidence-Based Medicine ,Models, Statistical ,Dengue epidemics ,business.industry ,Public health ,Public Health, Environmental and Occupational Health ,Outbreak ,Dengue outbreaks ,medicine.disease ,wc_532 ,3. Good health ,Latin America ,Population Surveillance ,Preparedness ,business ,Research Article - Abstract
BACKGROUND\ud The increasing frequency and intensity of dengue outbreaks in endemic and non-endemic countries requires a rational, evidence based response. To this end, we aimed to collate the experiences of a number of affected countries, identify strengths and limitations in dengue surveillance, outbreak preparedness, detection and response and contribute towards the development of a model contingency plan adaptable to country needs.\ud \ud METHODS\ud The study was undertaken in five Latin American (Brazil, Colombia, Dominican Republic, Mexico, Peru) and five in Asian countries (Indonesia, Malaysia, Maldives, Sri Lanka, Vietnam). A mixed-methods approach was used which included document analysis, key informant interviews, focus-group discussions, secondary data analysis and consensus building by an international dengue expert meeting organised by the World Health Organization, Special Program for Research and Training in Tropical Diseases (WHO-TDR).\ud \ud RESULTS\ud Country information on dengue is based on compulsory notification and reporting ("passive surveillance"), with laboratory confirmation (in all participating Latin American countries and some Asian countries) or by using a clinical syndromic definition. Seven countries additionally had sentinel sites with active dengue reporting, some also had virological surveillance. Six had agreed a formal definition of a dengue outbreak separate to seasonal variation in case numbers. Countries collected data on a range of warning signs that may identify outbreaks early, but none had developed a systematic approach to identifying and responding to the early stages of an outbreak. Outbreak response plans varied in quality, particularly regarding the early response. The surge capacity of hospitals with recent dengue outbreaks varied; those that could mobilise additional staff, beds, laboratory support and resources coped best in comparison to those improvising a coping strategy during the outbreak. Hospital outbreak management plans were present in 9/22 participating hospitals in Latin-America and 8/20 participating hospitals in Asia.\ud \ud CONCLUSIONS\ud Considerable variation between countries was observed with regard to surveillance, outbreak detection, and response. Through discussion at the expert meeting, suggestions were made for the development of a more standardised approach in the form of a model contingency plan, with agreed outbreak definitions and country-specific risk assessment schemes to initiate early response activities according to the outbreak phase. This would also allow greater cross-country sharing of ideas.
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- 2013
184. A Pilot Study to Assess Safety and Feasibility of Intrathecal Immunoglobulin for the Treatment of Adults with Tetanus.
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Huynh Thi Loan, Lam Minh Yen, Kestelyn, Evelyne, Van Hao, Nguyen, Nguyen Thi Hoang Mai, Duong Bich Thuy, Ha Thi Hai Duong, Nguyen Thi Phuong Dung, Nguyen Hoan Phu, Pham Thi Lieu, Tran Tan Thanh, Geskus, Ronald, van Doorn, H. Rogier, Le Van Tan, Wyncoll, Duncan, Day, Nicholas P. J., Hien, Tran Tinh, Thwaites, Guy E., Nguyen Van Vinh Chau, and Thwaites, C. Louise
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- 2018
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185. The prevalence, incidence and prevention of Plasmodium falciparum infections in forest rangers in Bu Gia Map National Park, Binh Phuoc province, Vietnam: a pilot study.
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Do Hung Son, Nguyen Thuy-Nhien, von Seidlein, Lorenz, Truong Le Phuc-Nhi, Ngo Thi Phu, Nguyen Thi Kim Tuyen, Nguyen Huyen Tran, Nguyen Van Dung, Bui Van Quan, Day, Nicholas P. J., Dondorp, Arjen M., White, Nicholas J., Thwaites, Guy E., and Hien, Tran Tinh
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FOREST rangers ,MALARIA prevention ,PLASMODIUM falciparum ,DISEASE prevalence ,MALARIA treatment - Abstract
Background: Prophylaxis for high-risk populations, such as forest workers, could be one component for malaria elimination in the Greater Mekong Sub-region. A study was conducted to assess the malaria incidence in forest rangers and the feasibility of malaria prophylaxis for rangers sleeping in forest camps. Methods: Forest rangers deployed in the Bu Gia Map National Park, Vietnam were invited to participate in the study. Plasmodium infections were cleared using presumptive treatment, irrespective of malaria status, with a 3-day course dihydroartemisinin/piperaquine (DP) and a 14-day course of primaquine. Before returning to the forest, study participants were randomly allocated to a 3-day course of DP or placebo. Fifteen days after returning from their forest deployment the participants were tested for Plasmodium infections using uPCR. Results: Prior to treatment, 30 of 150 study participants (20%) were found to be infected with Plasmodium. Seventeen days (median) after enrolment the rangers were randomized to DP or placebo 2 days before returning to forest camps where they stayed between 2 and 20 days (median 9.5 days). One ranger in the DP-prophylaxis arm and one in the placebo arm were found to be infected with Plasmodium falciparum 15 days (median) after returning from the forest. The evaluable P. falciparum isolates had molecular markers indicating resistance to artemisinins (K13-C580Y) and piperaquine (plasmepsin), but none had multiple copies of pfmdr1 associated with mefloquine resistance. Conclusion: Anti-malarial prophylaxis in forest rangers is feasible. The findings of the study highlight the threat of multidrug-resistant malaria. [ABSTRACT FROM AUTHOR]
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- 2017
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186. Short-course primaquine for the radical cure of Plasmodium vivaxmalaria: a multicentre, randomised, placebo-controlled non-inferiority trial
- Author
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Taylor, Walter R J, Thriemer, Kamala, von Seidlein, Lorenz, Yuentrakul, Prayoon, Assawariyathipat, Thanawat, Assefa, Ashenafi, Auburn, Sarah, Chand, Krisin, Chau, Nguyen Hoang, Cheah, Phaik Yeong, Dong, Le Thanh, Dhorda, Mehul, Degaga, Tamiru Shibru, Devine, Angela, Ekawati, Lenny L, Fahmi, Fahmi, Hailu, Asrat, Hasanzai, Mohammad Anwar, Hien, Tran Tinh, Khu, Htee, Ley, Benedikt, Lubell, Yoel, Marfurt, Jutta, Mohammad, Hussein, Moore, Kerryn A, Naddim, Mohammad Nader, Pasaribu, Ayodhia Pitaloka, Pasaribu, Syahril, Promnarate, Cholrawee, Rahim, Awab Ghulam, Sirithiranont, Pasathron, Solomon, Hiwot, Sudoyo, Herawati, Sutanto, Inge, Thanh, Ngo Viet, Tuyet-Trinh, Nguyen Thi, Waithira, Naomi, Woyessa, Adugna, Yamin, Fazal Yamin, Dondorp, Arjen, Simpson, Julie A, Baird, J Kevin, White, Nicholas J, Day, Nicholas P, and Price, Ric N
- Abstract
Primaquine is the only widely used drug that prevents Plasmodium vivaxmalaria relapses, but adherence to the standard 14-day regimen is poor. We aimed to assess the efficacy of a shorter course (7 days) of primaquine for radical cure of vivax malaria.
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- 2019
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187. Typhoid Fever and Genetic Polymorphisms at the Natural Resistance-Associated Macrophage Protein 1
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Dunstan, Sarah J., Ho, Vo An, Duc, Chau Minh, Lanh, Mai Ngoc, Thanh Phuong, Cao Xuan, Luxemburger, Christine, Wain, John, Dudbridge, Frank, Peacock, Christopher S., House, Deborah, Parry, Christopher, Hien, Tran Tinh, Dougan, Gordon, Farrar, Jeremy, and Blackwell, Jenefer M.
- Subjects
Typhoid fever -- Risk factors ,Salmonella typhimurium -- Genetic aspects ,Genetic polymorphisms -- Genetic aspects ,Natural immunity -- Genetic aspects ,Health - Published
- 2001
188. The epidemiology of subclinical malaria infections in South-East Asia: findings from cross-sectional surveys in Thailand–Myanmar border areas, Cambodia, and Vietnam
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Imwong, Mallika, primary, Nguyen, Thuy Nhien, additional, Tripura, Rupam, additional, Peto, Tom J., additional, Lee, Sue J., additional, Lwin, Khin Maung, additional, Suangkanarat, Preyanan, additional, Jeeyapant, Atthanee, additional, Vihokhern, Benchawan, additional, Wongsaen, Klanarong, additional, Van Hue, Dao, additional, Dong, Le Thanh, additional, Nguyen, Tam-Uyen, additional, Lubell, Yoel, additional, von Seidlein, Lorenz, additional, Dhorda, Mehul, additional, Promnarate, Cholrawee, additional, Snounou, Georges, additional, Malleret, Benoit, additional, Rénia, Laurent, additional, Keereecharoen, Lilly, additional, Singhasivanon, Pratap, additional, Sirithiranont, Pasathorn, additional, Chalk, Jem, additional, Nguon, Chea, additional, Hien, Tran Tinh, additional, Day, Nicholas, additional, White, Nicholas J., additional, Dondorp, Arjen, additional, and Nosten, Francois, additional
- Published
- 2015
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189. Phylodynamics of Enterovirus A71-Associated Hand, Foot, and Mouth Disease in Viet Nam
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Geoghegan, Jemma L., primary, Tan, Le Van, additional, Kühnert, Denise, additional, Halpin, Rebecca A., additional, Lin, Xudong, additional, Simenauer, Ari, additional, Akopov, Asmik, additional, Das, Suman R., additional, Stockwell, Timothy B., additional, Shrivastava, Susmita, additional, Ngoc, Nghiem My, additional, Uyen, Le Thi Tam, additional, Tuyen, Nguyen Thi Kim, additional, Thanh, Tran Tan, additional, Hang, Vu Thi Ty, additional, Qui, Phan Tu, additional, Hung, Nguyen Thanh, additional, Khanh, Truong Huu, additional, Thinh, Le Quoc, additional, Nhan, Le Nguyen Thanh, additional, Van, Hoang Minh Tu, additional, Viet, Do Chau, additional, Tuan, Ha Manh, additional, Viet, Ho Lu, additional, Hien, Tran Tinh, additional, Chau, Nguyen Van Vinh, additional, Thwaites, Guy, additional, Grenfell, Bryan T., additional, Stadler, Tanja, additional, Wentworth, David E., additional, Holmes, Edward C., additional, and Van Doorn, H. Rogier, additional
- Published
- 2015
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190. A Prospective Multi-Center Observational Study of Children Hospitalized with Diarrhea in Ho Chi Minh City, Vietnam
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Thompson, Corinne N., primary, Minh, Pham Van, additional, Phuc, Hoang Le, additional, Thuong, Tang Chi, additional, Vinh, Ha, additional, Tu, Le Thi Phuong, additional, Thuy, Cao Thu, additional, Chau, Nguyen Van Vinh, additional, Tuyet, Pham Thi Ngoc, additional, Phan, My V. T., additional, Ngoc, Nguyen Minh, additional, Duy, Pham Thanh, additional, Nga, Tran Vu Thieu, additional, Duong, Vu Thuy, additional, Dung, Tran Thi Ngoc, additional, Chinh, Nguyen Tran, additional, Rabaa, Maia A., additional, Jenkins, Claire, additional, Hien, Tran Tinh, additional, Campbell, James I., additional, Vinh, Nguyen Thanh, additional, Phat, Voong Vinh, additional, Tuan, Ha Manh, additional, Baker, Stephen, additional, Tuyen, Ha Thanh, additional, Nga, Tran Thi Thu, additional, Yoshihara, Keisuke, additional, Thwaites, Guy, additional, and Hoang, Nguyen Van Minh, additional
- Published
- 2015
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191. The clinical implications of thrombocytopenia in adults with severe falciparum malaria: a retrospective analysis
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Hanson, Josh, primary, Phu, Nguyen Hoan, additional, Hasan, Mahtab Uddin, additional, Charunwatthana, Prakaykaew, additional, Plewes, Katherine, additional, Maude, Richard J, additional, Prapansilp, Panote, additional, Kingston, Hugh WF, additional, Mishra, Saroj K, additional, Mohanty, Sanjib, additional, Price, Ric N, additional, Faiz, M Abul, additional, Dondorp, Arjen M, additional, White, Nicholas J, additional, Hien, Tran Tinh, additional, and Day, Nicholas PJ, additional
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- 2015
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192. LTA4H Genotype Is Associated with Susceptibility to Bacterial Meningitis but Is Not a Critical Determinant of Outcome
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Dunstan, Sarah J., primary, Tram, Trinh Thi Bich, additional, Thwaites, Guy E., additional, Chau, Tran Thi Hong, additional, Phu, Nguyen Hoan, additional, Hien, Tran Tinh, additional, Farrar, Jeremy J., additional, Wolbers, Marcel, additional, and Mai, Nguyen Thi Hoang, additional
- Published
- 2015
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193. Plasmodium falciparum field isolates from areas of repeated emergence of drug resistant malaria show no evidence of hypermutator phenotype
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Brown, Tyler S., primary, Jacob, Christopher G., additional, Silva, Joana C., additional, Takala-Harrison, Shannon, additional, Djimdé, Abdoulaye, additional, Dondorp, Arjen M., additional, Fukuda, Mark, additional, Noedl, Harald, additional, Nyunt, Myaing Myaing, additional, Kyaw, Myat Phone, additional, Mayxay, Mayfong, additional, Hien, Tran Tinh, additional, Plowe, Christopher V., additional, and Cummings, Michael P., additional
- Published
- 2015
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194. A real-time RT-PCR for detection of clade 1 and 2 H5N1 Influenza A virus using Locked Nucleic Acid (LNA) TaqMan probes
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Heiman F. L. Wertheim, Harun Syahrial, Jeremy Farrar, Trung Nguyen Vu, Chau Nguyen Van Vinh, Hien Tran Tinh, Ha Do Quang, H. Rogier van Doorn, Hien Vo Minh, Ngoc Nghiem My, Thanh Tran Tan, Menno D. de Jong, Hana Apsari Pawestri, Endang Rahayu Sedyaningsih, Faculteit der Geneeskunde, Other departments, Amsterdam institute for Infection and Immunity, and Medical Microbiology and Infection Prevention
- Subjects
viruses ,Oligonucleotides ,Hemagglutinin Glycoproteins, Influenza Virus ,Biology ,medicine.disease_cause ,Sensitivity and Specificity ,Virus ,law.invention ,lcsh:Infectious and parasitic diseases ,Plasmid ,law ,Virology ,Influenza, Human ,Influenza A virus ,medicine ,TaqMan ,Humans ,lcsh:RC109-216 ,Locked nucleic acid ,Polymerase chain reaction ,Conserved Sequence ,Influenza A Virus, H5N1 Subtype ,Reverse Transcriptase Polymerase Chain Reaction ,Methodology ,virus diseases ,Molecular biology ,Influenza A virus subtype H5N1 ,Infectious Diseases ,Real-time polymerase chain reaction ,Vietnam ,Oligonucleotide Probes - Abstract
Background The emergence and co-circulation of two different clades (clade 1 and 2) of H5N1 influenza viruses in Vietnam necessitates the availability of a diagnostic assay that can detect both variants. Results We developed a single real-time RT-PCR assay for detection of both clades of H5N1 viruses, directly from clinical specimens, using locked nucleic acid TaqMan probes. Primers and probe used in this assay were designed based on a highly conserved region in the HA gene of H5N1 viruses. The analytical sensitivity of the assay was < 0.5 PFU and 10 - 100 ssDNA plasmid copies. A total of 106 clinical samples (58 from patients infected with clade 1, 2.1 or 2.3 H5N1 viruses and 48 from uninfected or seasonal influenza A virus-infected individuals) were tested by the assay. The assay showed 97% concordance with initial diagnostics for H5 influenza virus infection with a specificity of 100%. Conclusions This assay is a useful tool for diagnosis of H5N1 virus infections in regions where different genetic clades are co-circulating.
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- 2010
195. CryptoDex: A randomised, double-blind, placebo-controlled phase III trial of adjunctive dexamethasone in HIV-infected adults with cryptococcal meningitis: study protocol for a randomised control trial
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Day, Jeremy, primary, Imran, Darma, additional, Ganiem, Ahmed Rizal, additional, Tjahjani, Natriana, additional, Wahyuningsih, Retno, additional, Adawiyah, Robiatul, additional, Dance, David, additional, Mayxay, Mayfong, additional, Newton, Paul, additional, Phetsouvanh, Rattanaphone, additional, Rattanavong, Sayaphet, additional, Chan, Adrienne K, additional, Heyderman, Robert, additional, van Oosterhout, Joep J, additional, Chierakul, Wirongrong, additional, Day, Nick, additional, Kamali, Anatoli, additional, Kibengo, Freddie, additional, Ruzagira, Eugene, additional, Gray, Alastair, additional, Lalloo, David G, additional, Beardsley, Justin, additional, Binh, Tran Quang, additional, Chau, Tran Thi Hong, additional, Chau, Nguyen Van Vinh, additional, Cuc, Ngo Thi Kim, additional, Farrar, Jeremy, additional, Hien, Tran Tinh, additional, Van Kinh, Nguyen, additional, Merson, Laura, additional, Phuong, Lan, additional, Tho, Loc Truong, additional, Thuy, Pham Thanh, additional, Thwaites, Guy, additional, Wertheim, Heiman, additional, and Wolbers, Marcel, additional
- Published
- 2014
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196. Variation at HLA-DRB1 is associated with resistance to enteric fever
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Dunstan, Sarah J, primary, Hue, Nguyen Thi, additional, Han, Buhm, additional, Li, Zheng, additional, Tram, Trinh Thi Bich, additional, Sim, Kar Seng, additional, Parry, Christopher M, additional, Chinh, Nguyen Tran, additional, Vinh, Ha, additional, Lan, Nguyen Phu Huong, additional, Thieu, Nga Tran Vu, additional, Vinh, Phat Voong, additional, Koirala, Samir, additional, Dongol, Sabina, additional, Arjyal, Amit, additional, Karkey, Abhilasha, additional, Shilpakar, Olita, additional, Dolecek, Christiane, additional, Foo, Jia Nee, additional, Phuong, Le Thi, additional, Lanh, Mai Ngoc, additional, Do, Tan, additional, Aung, Tin, additional, Hon, Do Nu, additional, Teo, Yik Ying, additional, Hibberd, Martin L, additional, Anders, Katherine L, additional, Okada, Yukinori, additional, Raychaudhuri, Soumya, additional, Simmons, Cameron P, additional, Baker, Stephen, additional, de Bakker, Paul I W, additional, Basnyat, Buddha, additional, Hien, Tran Tinh, additional, Farrar, Jeremy J, additional, and Khor, Chiea Chuen, additional
- Published
- 2014
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197. Avian influenza H5N1 and healthcare workers
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Schultsz, Constance, Dong, Vo Cong, Chau, Nguyen Van Vinh, Le, Nguyen Thi Hanh, Lim, Wilina, Thanh, Tran Tan, Dolecek, Christiane, de Jong, Menno D., Hien, Tran Tinh, and Farrar, Jeremy
- Abstract
To the Editor: Since January 2004, 35 human cases of avian influenza A virus H5N1 have been reported in Vietnam. Human-to-human transmission of H5N1 is a major concern, particularly because [...]
- Published
- 2005
198. PATHOGENESIS OF HAEMORRHAGE ASSOCIATED WITH DENGUE INFECTION IN ADULTS IN VIETNAM
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Trung, Dinh The, Hien, Tran Tinh, Thao, Le Thi Thu, Dung, Nguyen Minh, Ngoc, Tran, Goldin, Robert, Tuddenham, Edward, Cameron P. Simmons, Farrar, Jeremy, and Wills, Bridget
- Published
- 2008
199. Gene expression programs in adults with acute dengue infections
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Popper, Stephen, Cameron P. Simmons, Dolecek, Christiane, Chau, Tran Nguyen Bich, Griffiths, Michael, Dung, Nguyen Thi Phuong, Long, Truong Hoang, Hoang, Dang Minh, Vinh Chau, Nguyen, Thao, Le Thi Thu, Hien, Tran Tinh, Relman, David A., and Farrar, Jeremy
- Published
- 2006
200. Community perceptions of targeted anti-malarial mass drug administrations in two provinces in Vietnam: a quantitative survey.
- Author
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Thuy-Nhien Nguyen, Huong Thu, Pham N., Ngo Trong Hung, Do Hung Son, Nguyen Thanh Tien, Van Dung, Nguyen, Huynh Hong Quang, von Seidlein, Lorenz, Phaik Yeong Cheah, Dondorp, Arjen M., J. Day, Nicholas P., White, Nicholas J., and Hien, Tran Tinh
- Subjects
DRUG administration ,ANTIMALARIALS ,DRUG efficacy ,COMMUNITY health workers ,PUBLIC health - Abstract
Background: As part of a targeted malaria elimination project, mass drug administrations (MDAs) were conducted in Vietnam. The impact of MDAs on malaria transmission depends largely on the efficacy of the anti-malarial drug regimen, the malaria epidemiology in the site and the population coverage. To explore why some people participate in MDAs and others do not, a quantitative survey of the villagers' perceptions was undertaken in Vietnam. Methods: In 2013/2014 MDAs were conducted in a village in Binh Phuoc province and a village in Ninh Thuan province. Within three months of the drug administration, 59 respondents in a village in Binh Phuoc and 79 respondents in a village in Ninh Thuan were randomly selected and interviewed. Results: Comprehension of the purpose of the intervention was of paramount importance for participation in the intervention. Respondents aware that the intervention aims to protect against malaria were significantly more likely to participate than respondents who were unaware of the MDA's purpose. Secondly, how and by whom villagers were informed was critical for participation. There was a strong association between sensitization by an informant such as a member of the local health team with participation in the intervention. Conclusions: The study suggests several approaches to increase participation in mass drug administration campaigns. Training trustworthy informants to sensitize the study population is critical to maximize village participation in this setting. To achieve high coverage the entire community must understand and agree with the intervention. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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