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• A decentralized system was set up to transfer food waste to electricity and heat. • 30 kg food waste produced a methane yield of 0.55 L CH 4 /g VS in a current system. • A scale-up system was simulated to output 74.80kWh electricity at 500 kg feed per day. • Electrical and thermal energy outputs of optimized system increased by 135% and 87%. Biogas derived from the decentralized anaerobic digestion (AD) of food waste may be used to generate electrical and thermal energy for nearby residents through combined heat and power generation technology. A mobile food-waste-to-energy system in conjunction with an anaerobic digester and biogas engine was developed for food waste (FW) treatment and energy output. The scale-up was simulated to explore the methane yield and energy flow at different ambient temperatures of 0 °C, 10 °C, 20 °C, 30 °C and different feedstock of 200 kg, 500 kg FW. Accordingly, the results demonstrated that the installed system with a 1 m3 digester had a methane yield of 0.55L CH 4 /g VS when the feedstock load was 30 kg FW/d and the Organic Load Rate (OLR) was 5.4 g VS /L, while the thermal and electrical efficiencies of this system were 31% and 16%, respectively. The corresponding exhaust gas of the thermal energy from the biogas combustion was recovered by a heat exchanger in order to keep the digester at mesophilic conditions. The simulations carried out on the scale-up systems under loads of 200 kg FW/d and 500 kg FW/d achieved a thermal energy balance at different ambient temperatures of 0 °C, 10 °C, 20 °C and 30 °C. Improvements were proposed in the optimized system by optimizing pre-treatment facilities as well as the AD reactor, biogas engine and heat exchanger. The net thermal and electrical energy output of the optimized system at 500 kg FW/d were 175.93kWh and 163.90kWh, respectively. The energy generated by the FW-to-energy system illustrated by the Sankey diagrams depicts that was enough for operation without the need of extra heat and a power supply, moreover, the surplus energy may be utilized for its neighboring communities. In addition, the optimized system under the same OLR were increased by 87% and 135%, regards the thermal and electrical energy outputs, respectively in comparison with the current system. [ABSTRACT FROM AUTHOR]

• Mixing and activated carbon nexus was studied in thermophilic anaerobic digestion. • The optimum mixing time of 120 s/h were obtained via CFD modelling. • Coupling of activated carbon and semi-continuous mixing increased by 20% of CH 4. • Starting-up strategy - continuous mixing followed by intermittent mixing. • Metabolic pathways and functional genes of microbial communities were analyzed. This study evaluated the mixing – activate carbon nexus in anaerobic digestion with the aim of accelerating start-up of thermophilic anaerobic co-digestion of food waste and chicken manure using mesophilic anaerobic sludge as inoculum. Results showed that the methane yield in the continuous stirred reactor is 71.3% higher than that of intermittent agitated reactor, and the addition of activated carbon can further improve the yield of methane by 18.2%. Continuous mixing mode followed by intermittent mixing was proved to be an alternative strategy to accelerate start-up of thermophilic anaerobic digestion. The optimum mixing time of 120 s/hour were obtained using computational fluid dynamics modeling. Analysis of genomic annotation metabolism indicated that the addition of activated carbon enhanced the dominant metabolism pathways of amino acid, methane and energy. Results of enzymes gene expression suggested that carbohydrates esterases, glycoside hydrolases and glycosyl transferases were dominant, respectively. [ABSTRACT FROM AUTHOR]

• Algal biochar enhanced co-digestion of algal biomass and food waste for biomethane. • 75% algal biomass + 25% food waste mixture co-digestion showed a synergistic effect. • Average methane yields enhanced by 12–54% due to 15 g/L of algal biochar amendment. • Proteiniphilum enriched in mesophilic reactor like Defluviitoga in thermophilic one. • Biochar shifted methanogens towards a balance of two flexible methanogenic pathways. The impact of algal biochar addition on mesophilic and thermophilic anaerobic co-digestion of algal biomass and food waste was investigated with a focus on semi-continuous operations and functional microbial communities. Under batch co-digestion, the highest co-digestion synergy was observed for a mixture of 25% food waste and 75% algal biomass. During semi-continuous co-digestion of 25% food waste-75% algal biomass mixture, biochar amended digesters exhibited a 12–54% increase in average methane yield (275.8–394.6 mL/gVS) compared to the controls. Elevated temperature induced narrow distributions of volatile fatty acids (VFAs) by inhibiting the production of branched VFAs. Genus Proteiniphilum was selectively enriched by 3.2 folds in mesophilic digesters with biochar amendment while genus Defluviitoga was selectively enriched in thermophilic digesters due to elevated temperature. Methanogenic communities were significantly different in mesophilic and thermophilic digesters. Biochar amendment contributed to shifts in the predominant methanogens leading to a more balanced state of two methanogenic pathways. [ABSTRACT FROM AUTHOR]

This study emphasized the synergistic production of bioprocessible lignin and carbohydrates during a sequential liquid hot water and alkali pretreatment of lignocellulose, facilitating their subsequent individual fermentation. Increasing the dose of alkaline lignin from 0 to 8 g/L inhibited cell growth in anaerobic digestion, with varying levels of inhibition observed in the following order: hydrolytic bacteria < acidogens < acetogens. Alkali pretreatment was adapted to maximize yields of bioprocessible lignin liquor without compromising utilization of the carbohydrates. Increasing the NaOH dose from 50 to 200 mg/g-feedstock monotonically improved lignin yields, but further increases in alkali loading led to a decline in lignin recovery. Volatile fatty acids production from anaerobic digestion of the carbohydrate moiety consistently increased with higher NaOH doses. The optimal conditions for maximizing lignin yields were determined to be 105 °C for 30 min, with NaOH loading in the range of 150-200 mg/g-feedstock, resulting in approximately 80 % lignin recovery, of which 35 % was biologically utilizable. Liquid hot water treatment prior to alkali pretreatment was confirmed as necessary to preserve carbohydrates of 0.1 g/g-feedstock at a low temperature of 70 °C. These findings are crucial for economically producing bioprocessible lignin without carbohydrate loss, a key step towards achieving full lignocellulose valorization., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Pseudomonas putida degraded 35 % of compounds in alkali-pretreated lignin liquor under nitrogen-replete conditions but with low polyhydroxyalkanoates (PHA) production, while limiting nitrogen supplement improved PHA content (PHA/dry cell weight) to 43 % at the expense of decreased lignin degradation of 22 %. Increase of initial cell biomass (0.1-1.5 g/L) monotonically improved the lignin degradation from 22 % to 33 % under nitrogen-limited conditions. Hierarchical utilization of heterogenous compounds under cell growth restricted conditions has been unveiled - simple carbon sources were prioritized for valorization, followed by aromatic compounds bioconversion. Based on the results of hierarchy and leveraging the initial bacterial biomass, acetate was augmented to facilitate one-pot lignin bioconversion under nitrogen-limited conditions. This approach improved lignin bioconversion closer to its upper degradation limit of 35 %, concomitant with PHA yield of 39 mg/g-lignin. Anaerobic digestion of lignocellulose was redesigned to favor acetate-type fermentation, with acetate constituting 91 wt%, providing an economic source of acetate., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2025. Published by Elsevier Ltd.)

Lignocellulosic biomass wastes are abundant resources that are usually valorized for methane-rich biogas via anaerobic digestion. Conversion of lignocellulose into volatile fatty acids (VFA) rather than biogas is attracting attention due to the higher value-added products that come with VFA utilization. This review consolidated the latest studies associated with characteristics of lignocellulosic biomass, the effects of process parameters during acidogenic fermentation, and the intensification strategies to accumulate more VFA. The differences between anaerobic digestion technology and acidogenic fermentation technology were discussed. Performance-enhancing strategies surveyed included (1) alkaline fermentation; (2) co-digestion and high solid-state fermentation; (3) pretreatments; (4) use of high loading rate and short retention time; (5) integration with electrochemical technology, and (6) adoption of membrane bioreactors. The recommended operations include: mesophilic temperature (thermophilic for high loading rate fermentation), C/N ratio (20-40), OLR (< 12 g volatile solids (VS)/(L·d)), and the maximum HRT (8-12 days), alkaline fermentation, membrane technology or electrodialysis recovery. Lastly, perspectives were put into place based on critical analysis on status of acidogenic fermentation of lignocellulosic biomass wastes for VFA production., (© 2021. The Author(s).)

Acidogenic fermentation of food waste for production of volatile fatty acids (VFAs) contributes to both food waste minimization and resource recovery. To gain knowledge on functional bacterial communities and facilitate continuous production of VFAs, this research firstly studied the effects of initial pH values (i.e. 5, 6 and 7) and temperatures (i.e. 35 °C and 55 °C) on VFAs production, distribution, and bacterial communities during acidogenic fermentation of food waste. The optimal conditions were determined as pH 7 and 35 °C, corresponding to the highest total VFAs yield of 11.8 g COD/L with major components of acetic, propionic and butyric acid. Bioinformatic analysis showed that the relative abundance of the dominant bacterial classes (e.g. Clostridia, Bacteroidia and Bacilli) were changed by the initial pH values in both mesophilic and thermophilic reactors. NMDS analysis confirmed a significant difference between mesophilic and thermophilic communities. Finally, the feasibility of continuous production and recovery of VFAs was validated using a two-phase leachate bed bioreactor at the optimal conditions. Average concentration and yield of the total VFAs in the continuous operation were 6.3 g COD/L and 0.29 g VFA/g VS added , respectively. The findings in this study could provide pivotal technical supports for potential pilot- and commercial-scale biorefinery plants for VFAs production from food waste., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

Laboratory semi-continuous anaerobic digestion (AD) experiments were performed to investigate the effects of different supplements on the AD performance of food waste, specifically activated carbon (AC), encapsulated trace element additive (ETEA) and a combination of AC + ETEA. Results indicated that the operation stability of AD was enhanced through the addition of the additives. Compared with the control digester without any additive, AC, ETEA, and AC + ETEA increased the average methane yield by 34%, 22% and 50%, respectively. Chemical speciation analyses indicated that AC + ETEA supplementation increased the proportion of water soluble form of Ni by 11-23%, compared to ETEA single addition. Real-time PCR analyses showed that AC and ETEA supplementation synergistically facilitated the growth of bacterial and archaeal communities. Microbial community structure analysis revealed that AC + ETEA favored the enrichment of hydrolytic, acidogenic and acetogenic bacteria and methanogens., (Copyright © 2019 Elsevier Ltd. All rights reserved.)