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In this paper, I investigate the origins of banality and the reasons why some phenomena appear banal to us. I discuss the issue by analysing three interrelated areas of aesthetic investigation: artworks, everyday objects, and banal things. By identifying the source of banality, my goal is to understand what makes banal things different from other kinds of things. I consider the following questions: 1) when, why, and how does an object become banal?; 2) what happens when something becomes banal?; 3) are banal things aesthetically appealing? Drawing on Wolfgang Welsch’s notion of anesthetization and Walter Benjamin’s account of aura, I argue that banality consists in the absence of both an ontological and an axiological character in objects, which makes them appear trivial or insignificant to us. I conclude by showing that although art, everydayness, and banality represent different aesthetic dimensions, objects constantly move from one of these dimensions to the other.

Abstract Preparing and observing quantum states of nanoscale particles is a challenging task with great relevance for quantum technologies and tests of fundamental physics. In contrast to atomic systems with discrete transitions, nanoparticles exhibit a practically continuous absorption spectrum and thus their quantum dynamics cannot be easily manipulated. Here, we demonstrate that charged nanoscale dielectrics can be artificially endowed with a discrete level structure by coherently interfacing their rotational and translational motion with a superconducting qubit. We propose a pulsed scheme for the generation and read-out of motional quantum superpositions and entanglement between several levitated nanoparticles, providing an all-electric platform for networked hybrid quantum devices.

The image of the ancient past represents just a fragment. When we stand in front of such an image, we are standing in front of time. This text reflects the possibilities of aesthetic evaluation of ancient artifacts with the emphasis on the contextual perception of received phenomena. The defining conceptof context is based on Jan Mukařovsky’s approach. The aim of the paper is also to present aesthetic interpretation as a regular method of verifying prehistoric artifacts.

This study aims to compare reciprocal teaching styles influence, exercises and command on learning outcomes of pencak silat paleredan. Experimental research method with Pretest-Posttest Group Design research design. Samples that fulfilled the requirements were 38 people. The research found that 1) the influences of Teaching Style Reciprocal toward Learning Results Pencak Silat Tepak Paleredan, 2) There is the influence of Teaching Style Exercise on Learning Outcomes Pencak Silat Tepak Paleredan, 3) influences of Teaching Style Command on Learning Outcomes Pencak Silat Tepak Paleredan, 4) The teaching exercises style have more significant impact on learning outcomes martial test Tepak Paleredan compared Reciprocal teaching style and Command. Then it can be concluded that the three teaching styles have a significant influence on the learning outcomes of the Paleredan Pencak silat in the Universitas Nusa Cendana Penjaskesrek Academic Year 2019/2020

This research aims to reveal the differences between the performance of bachelor degree holder teachers and that of non-degree holder teachers of elementary schools in Kupang. It applies the survey method. The data were collected using a questionnaire. The questionnaire's validity was measured using the Product Moment Correlation formula, and its reliability was measured using the Alfa Cronbach formula. The data analysis used the non-parametric Mann-Whitney test with the help of the SPSS Program. The result of the study shows that there is a significant average difference between the teaching performance of bachelor degree holder teachers and that of non-degree holder teachers of elementary schools in Kupang. There is a significant average difference between bachelor degree holder teachers' personality competence and that of non-degree holder teachers. There is a significant average difference between the paedagogic competence of bachelor degree holder teachers and non-degree holder teachers. There is a significant average difference between bachelor degree holder teachers' social competence and that of non-degree holder teachers. Therefore, it is suggested that non-degree holder teachers keep improving their academic qualification and work performance through varieties of self-development efforts.

Background. Magnetic resonance imaging (MRI) offers the opportunity to quantify the vessel diameters in vivo. This technique can have a breakthrough impact on the evaluation, risk stratification and therapeutical planning in hemodynamic-related pathologies, e.g., arterial stenosis. However, its applicability in clinics is limi­ted due to the complex post-processing required to extract the information and the difficulty to synthesize the obtained data into clinical useful parameters. Objective. In this work, we use the vessel diameter distribution along its central line obtained with the use of MRI technology in order to detect the existence of stenosis in internal carotid arteries (ICA) and vertebral arteries (VA) with the minimal amount of False Negative predictions and to estimate the efficiency of therapy. Methods. Special normalized and smoothed characteristics will be used to develop the stenosis detection criteria which can be used for every artery separately and for both vessels simultaneously. Linear and non-linear characteristics were used to increase the reliability of diagnostics. Study is based on the Receiver Operating Characteristics (ROC) and optimization methods. Real diameter data of 10 patients (80 data sets) were used. Results. To detect stenosis, three different criteria have been proposed, based on the optimal smoothing parameters of vessel diameter distributions and the corresponding threshold values for linear and nonlinear characteristics. The use of the developed criteria allows increasing the reliability of stenosis detection. Conclusions. Different linear, non-linear, smoothed and non-smoothed parameters and ROC were applied to detect stenosis in internal carotid and vertebral arteries. It was shown that smoothed data are necessary for VA and the criterion applicable both for VA and ICA. For ICA it is possible to use initial (unsmoothed) data. Only one False Positive case was detected for every artery. Results of application of proposed criteria are presented, tested and discussed. For VA it is possible to use criteria 1 and 2 and smoothed normalized diameter data. For ICA criterion 2 can be recommended to detect long enough narrowing areas. To detect short zones of stenosis in ICA, the criterion 3 is useful, since it uses the non-smoothed diameter data.

This study aims to analyze the business view concerning the using the accommodation capacities in some central European countries, i.e. Austria, the Czech Republic and Slovakia in the NUTS-2 regional scope. The special attention is paid to Spain. The research is based on annual post-global economic crisis data. The authors apply a specific partial least squares (PLS) variant of multivariate methods, which relates many fundamental and derived tourism variables due to particular attention to using a weighting procedure. The authors determined that in order to encompass the territory predetermination for the best fit the changed conditions, the majority of significant cities have very good dynamics in capacity parameters and overnights for increasing the offers being greatly supplied by the annual changing number of visitors. However, Spain is substantially different from the other regions analyzed, forming ultimate conditions for mutual comparison. Moreover, the tracks of turning visitors into capital or significant cities, especially associated with the close natural attractions, are substantiated. The tourist’s resource potential specific only to the target region as well as relevant additional potential origins are examined on the sample of countries. Covering tourism as the world’s leading industry directly connected to accommodation tasks and a unique period examined, the results of this study can be used to formulate policy guidelines as well as to solve the tasks of attracting tourism and promote supply. AcknowledgmentThis research was funded by the Grant Agency of Academic Alliance under Grant Agreement number GA/13/2018.

BackgroundSpatial navigation impairment is a promising cognitive marker of Alzheimer’s disease (AD) that can reflect the underlying pathology.ObjectivesWe assessed spatial navigation performance in AD biomarker positive older adults with amnestic mild cognitive impairment (AD aMCI) vs. those AD biomarker negative (non-AD aMCI), and examined associations between navigation performance, MRI measures of brain atrophy, and cerebrospinal fluid (CSF) biomarkers.MethodsA total of 122 participants with AD aMCI (n = 33), non-AD aMCI (n = 31), mild AD dementia (n = 28), and 30 cognitively normal older adults (CN) underwent cognitive assessment, brain MRI (n = 100 had high-quality images for volumetric analysis) and three virtual navigation tasks focused on route learning (body-centered navigation), wayfinding (world-centered navigation) and perspective taking/wayfinding. Cognitively impaired participants underwent CSF biomarker assessment [amyloid-β1–42, total tau, and phosphorylated tau181 (p-tau181)] and amyloid PET imaging (n = 47 and n = 45, respectively), with a subset having both (n = 19).ResultsIn route learning, AD aMCI performed worse than non-AD aMCI (p < 0.001), who performed similarly to CN. In wayfinding, aMCI participants performed worse than CN (both p ≤ 0.009) and AD aMCI performed worse than non-AD aMCI in the second task session (p = 0.032). In perspective taking/wayfinding, aMCI participants performed worse than CN (both p ≤ 0.001). AD aMCI and non-AD aMCI did not differ in conventional cognitive tests. Route learning was associated with parietal thickness and amyloid-β1–42, wayfinding was associated with posterior medial temporal lobe (MTL) volume and p-tau181 and perspective taking/wayfinding was correlated with MRI measures of several brain regions and all CSF biomarkers.ConclusionAD biomarker positive and negative older adults with aMCI had different profiles of spatial navigation deficits that were associated with posterior MTL and parietal atrophy and reflected AD pathology.

The use of aluminium alloys for Additive Manufacturing is of high interest for advanced geometries and lightweight applications. In Directed Energy Deposition, a powder stock is processed with a laser beam, which offers a high process flexibility. However, aging of the powder feedstock during storage or after recycling remains fundamentally challenging for aluminium alloys because of their sensitivity to oxidation and porosity. In order to investigate these effects, AlSi10Mg powder batches were aged in different conditions and processed by Directed Energy Deposition. The results showed that powder aging does not significantly change the particle size or morphology, but it introduces more oxygen and hydrogen in the powder. The oxidation of the particles reduces the laser beam absorbance of the powder and increases wetting of the melt pool, which affects the track geometry. A 3.5 to 4.2 times higher porosity was observed in the material deposited from aged powder, which are most likely hydrogen pores caused by the increased hydrogen content in the aged powder. The tensile properties of the parts built with aged powder showed 19.0% lower yield strength, 14.2% lower ultimate strength and 99.2% higher elongation, which are most likely the results of the coarser microstructure and increased porosity.

During the Covid-19 pandemic, people have been exposed to vast amounts of misinformation. This “infodemic” has undermined key behavioural and pharmacological measures to contain the pandemic. In a cross-sectional survey of residents of Germany, we investigated the perceived prevalence of misinformation, the strategies people reported using to discern between true and false information, and individual differences in beliefs in misinformation at three time points from June 2020 to February 2021 (N = 3324). We observed four main results. First, there was an increase in the perceived prevalence of misinformation over time. Second, the most believed false claims included that the virus is no worse than the flu and that the EU has approved dangerous vaccines. Third, belief in misinformation was associated with support for the far-right AfD party; reliance on tabloids, neighbours and social media for information; lower levels of education; and migration background. Fourth, only about half of the respondents reported using strategies such as checking for consistency between different sources to identify misinformation. These results can inform the development of interventions, such as boosting the ability to discern accurate from misleading information, or enriching specific environments (e.g., neighbourhoods with high rates of migration) with accessible and high-quality information.

ObjectiveThe aim of this study was to investigate (a) macrophage migration inhibitory factor (MIF) levels in polytrauma patients and rats after haemorrhagic shock (HS), (b) the potential of the MIF inhibitor ISO-1 to reduce multiple organ dysfunction syndrome (MODS) in acute (short-term and long-term follow-up) HS rat models and (c) whether treatment with ISO-1 attenuates NF-κB and NLRP3 activation in HS.BackgroundThe MODS caused by an excessive systemic inflammatory response following trauma is associated with a high morbidity and mortality. MIF is a pleiotropic cytokine which can modulate the inflammatory response, however, its role in trauma is unknown.MethodsThe MIF levels in plasma of polytrauma patients and serum of rats with HS were measured by ELISA. Acute HS rat models were performed to determine the influence of ISO-1 on MODS. The activation of NF-κB and NLRP3 pathways were analysed by western blot in the kidney and liver.ResultsWe demonstrated that (a) MIF levels are increased in polytrauma patients on arrival to the emergency room and in rats after HS, (b) HS caused organ injury and/or dysfunction and hypotension (post-resuscitation) in rats, while (c) treatment of HS-rats with ISO-1 attenuated the organ injury and dysfunction in acute HS models and (d) reduced the activation of NF-κB and NLRP3 pathways in the kidney and liver.ConclusionOur results point to a role of MIF in the pathophysiology of trauma-induced organ injury and dysfunction and indicate that MIF inhibitors may be used as a potential therapeutic approach for MODS after trauma and/or haemorrhage.

Summary: Pediatric renal cell carcinomas (RCC) differ from their adult counterparts not only in histologic subtypes but also in clinical characteristics and outcome. However, the underlying biology is still largely unclear. For this reason, we performed whole-exome and transcriptome sequencing analyses on a cohort of 25 pediatric RCC patients with various histologic subtypes, including 10 MiT family translocation (MiT) and 10 papillary RCCs. In this cohort of pediatric RCC, we find only limited genomic overlap with adult RCC, even within the same histologic subtype. Recurrent somatic mutations in genes not previously reported in RCC were detected, such as in CCDC168, PLEKHA1, VWF, and MAP3K9. Our papillary pediatric RCCs, which represent the largest cohort to date with comprehensive molecular profiling in this age group, appeared as a distinct genomic subtype differing in terms of gene mutations and gene expression patterns not only from MiT-RCC but also from their adult counterparts.

A triplet (a,b,c) of positive integers is said to be product-feasible if there exist algebraic numbers α, β and γ of degrees (over Q) a, b and c, respectively, such that αβγ=1. This work extends the investigation of product-feasible triplets started by Drungilas, Dubickas and Smyth. More precisely, for all but five positive integer triplets (a,b,c) with a≤b≤c and b≤7, we decide whether it is product-feasible. Moreover, in the Appendix we give an infinite family or irreducible compositum-feasible triplets and propose a problem to find all such triplets.

The Internet of Things (IoT) has been shown to be very valuable for Business Process Management (BPM), for example, to better track and control process executions. While IoT actuators can automatically trigger actions, IoT sensors can monitor the changes in the environment and the humans involved in the processes. These sensors produce large amounts of discrete and continuous data streams, which hold the key to understanding the quality of the executed processes. However, to enable this understanding, it is needed to have a joint representation of the data generated by the process engine executing the process, and the data generated by the IoT sensors. In this paper, we present an extension of the event log standard format XES called DataStream. DataStream enables the connection of IoT data to process events, preserving the full context required for data analysis, even when scenarios or hardware artifacts are rapidly changing. The DataStream extension is designed based on a set of goals and evaluated by creating two datasets for real-world scenarios from the transportation/logistics and manufacturing domains.

The Timepix3 is a hybrid pixellated radiation detector consisting of a 256 px × 256 px radiation-sensitive matrix. Research has shown that it is susceptible to energy spectrum distortion due to temperature variations. This can lead to a relative measurement error of up to 35% in the tested temperature range of 10 °C to 70 °C. To overcome this issue, this study proposes a complex compensation method to reduce the error to less than 1%. The compensation method was tested with different radiation sources, focusing on energy peaks up to 100 keV. The results of the study showed that a general model for temperature distortion compensation could be established, where the error in the X-ray fluorescence spectrum of Lead (74.97 keV) was reduced from 22% to less than 2% for 60 °C after the correction was applied. The validity of the model was also verified at temperatures below 0 °C, where the relative measurement error for the Tin peak (25.27 keV) was reduced from 11.4% to 2.1% at −40 °C. The results of this study demonstrate the effectiveness of the proposed compensation method and models in significantly improving the accuracy of energy measurements. This has implications for various fields of research and industry that require accurate radiation energy measurements and cannot afford to use power for cooling or temperature stabilisation of the detector.

Magnetic force microscopy (MFM) is a powerful extension of atomic force microscopy (AFM), which mostly uses nano-probes with functional coatings for studying magnetic surface features. Although well established, additional layers inherently increase apex radii, which reduce lateral resolution and also contain the risk of delamination, rendering such nano-probes doubtful or even useless. To overcome these limitations, we now introduce the additive direct-write fabrication of magnetic nano-cones via focused electron beam-induced deposition (FEBID) using an HCo3Fe(CO)12 precursor. The study first identifies a proper 3D design, confines the most relevant process parameters by means of primary electron energy and beam currents, and evaluates post-growth procedures as well. That way, highly crystalline nano-tips with minimal surface contamination and apex radii in the sub-15 nm regime are fabricated and benchmarked against commercial products. The results not only reveal a very high performance during MFM operation but in particular demonstrate virtually loss-free behavior after almost 8 h of continuous operation, thanks to the all-metal character. Even after more than 12 months of storage in ambient conditions, no performance loss is observed, which underlines the high overall performance of the here-introduced FEBID-based Co3Fe MFM nano-probes.

We explore the different notions of completeness applied in the EPR discussion following and amending the thorough analysis of Arthur Fine. To this aim, we propose a classification scheme for scientific theories that provides a methodology for analyzing the different levels at which interpretive approaches come into play. This allows us to contrast several concepts of completeness that operate on specific levels of the theory. We introduce the notion of theory completeness and compare it with the established notions of Born completeness, Schrödinger completeness and bijective completeness. We relate these notions to the recent concept of ψ-completeness and predictable completeness. The paper shows that the EPR argument contains conflicting versions of completeness. The confusion of these notions led to misunderstandings in the EPR debate and hindered its progress. Their clarification will thus contribute to recent debates on interpretational issues of quantum mechanics. Finally, we discuss the connection between the EPR paper and the Einstein–Rosen paper with regard to the question of completeness.

Compound casting is a process in which a single component is made from two metallic materials, such as aluminum and steel. Solid-liquid bimetallic compounds can be produced by suitable process control. This technology can reduce the number of joining processes, and the specific properties of the respective metal component can be used for specifically designed product properties, for example, where lightweight and high strength are needed. This paper presents an experimental methodology for producing a purely material-bonded bimetallic joint from cast aluminum and zinc-coated sheet steel in 3D sand casting using an inductive heating system. The process-related temperature characterisation in the compound zone is described using a heating test rig and temperature measurements. It shows that inductive preheating can only produce a material bond between the aluminum casting and the coated steel sheet. Shear tensile tests showed strengths between 15 MPa and 22 MPa. Laser surface pre-treatment using laser ablation cutting on the coated steel sheet was carried out to investigate the benefit of possible microform-locking. The results show a strength-reducing influence on the tensile shear tests. Micrographs showed the formation of Al4.5FeSi and Al7Fe2Si, as well as the formation of other undefined intermetallic phases. The thickness of the compound zone is 10 µm.

The Timepix3 readout ASIC chip is a hybrid pixelated radiation detector, designed at CERN, which contains a 256 px × 256 px matrix. Each of the 65,536 radiation-sensitive pixels can record an incoming particle, its energy deposition or time of arrival and measure them simultaneously. Since the detector is suitable for a wide range of applications from particle physics, national security and medicine to space science, it can be used in a wide range of temperatures. Until now, it has to be calibrated every time to the operating point of the application. This paper studies the possibility of energy measurement with Timepix3 equipped with a 500 m thick silicon sensor and MiniPIX readout interface in the temperatures between 10 ∘C and 70 ∘C with only one calibration. The detector has been irradiated by X-ray fluorescence photons in the energy range from 8 keV to 57 keV, and 31 keV to 81 keV photons from the 133Ba radioactive source. A deviation of 5% in apparent energy value may occur for a 10 ∘C change in temperature from the reference point, but, with the next temperature change, it can reach up to −30%. Moreover, Barium photons with an energy of 81 keV appear as deposited energy of only 55 keV at a detector temperature of 70 ∘C. An original compensation method that reduces the relative measurement error from −30% to less than 1% is presented in this paper.

This article was prompted by a gilt bronze lockplate in the Rijksmuseum, originally the decorative fastening of a chest and one of a large group of similar objects. Hardly any other metalwork design was more extensively reproduced in Italian Mannerism. Its success was based on the appealing design and the fact this type of lockplate offered the possibility of integrating coats of arms and thus personalizing a chest. The paper presents new examples not yet listed in Charles Avery’s comprehensive overview (2001), identifies a whole series of clients for these lockplates on the basis of heraldic and genealogical analyses and deduces from this an origin in Rome and a dating of the entire group (previously dated 1540) to the last third of the sixteenth century. It has been generally assumed that the specific function of these objects was to decorate marriage chests. Closer analysis argues against this thesis. The lockplate in the Rijksmuseum is particularly significant in this context. The coats of arms on its lateral cartouches identify the Roman Orazio Ruspoli and his wife Felice Cavalieri (marr. 1594) as the clients for the piece. Surprisingly, however, the crest on the hasp belongs to a family that was not related to this couple. A comparable finding is made for a lockplate in the National Gallery in Washington, which has also been misinterpreted so far. In this case, too, the coats of arms on the plate and on the hasp do not point to a family connection, but to neighbouring and presumably friendly families. The analysis of other examples, such as one in the Palazzo Venezia, confirms that these lockplates and the chests to which they were attached were not exclusively bound to the context of marriage. As travelling chests, which became must-have items for the Roman upper class, they seem to have been open to a variety of functions.

Infection and viral entry of SARS-CoV-2 crucially depends on the binding of its Spike protein to angiotensin converting enzyme 2 (ACE2) presented on host cells. Glycosylation of both proteins is critical for this interaction. Recombinant soluble human ACE2 can neutralize SARS-CoV-2 and is currently undergoing clinical tests for the treatment of COVID-19. We used 3D structural models and molecular dynamics simulations to define the ACE2 N-glycans that critically influence Spike-ACE2 complex formation. Engineering of ACE2 N-glycosylation by site-directed mutagenesis or glycosidase treatment resulted in enhanced binding affinities and improved virus neutralization without notable deleterious effects on the structural stability and catalytic activity of the protein. Importantly, simultaneous removal of all accessible N-glycans from recombinant soluble human ACE2 yields a superior SARS-CoV-2 decoy receptor with promise as effective treatment for COVID-19 patients.

Seagrass species play a critical role in the mitigation of climate change by acting as valuable carbon sinks and storage sites. Another important ecosystem service of this coastal vegetation is nutrient removal. However, coastal ecosystems are under increasing pressure of global warming and associated establishment of invasive species. To elucidate the respective contributions of seagrass species Posidonia oceanica and Cymodocea nodosa and the non-native macroalga Halimeda incrassata as primary producers and nutrient sinks in coastal habitats we conducted in-situ incubations in the North-western Mediterranean Sea. Measured metabolic activity and nutrient removal as well as calcification rates in these habitats over a 24 h period in spring and summer confirmed that the endemic seagrass P. oceanica represents a valuable ecosystem with high O2 production and considerable carbon capture. The documented regression of P. oceanica meadows with higher temperatures and decline in autotrophy as measured here causes concern for the continuity of ecosystem services rendered by this habitat throughout the Mediterranean Sea with progressing climate warming. In contrast, the enhanced performance of C. nodosa and the calcifying alga H. incrassata with increasing temperatures, under expected rates of future warming is uncertain to mitigate loss of productivity in case of a potential shift in marine vegetation. This could ultimately lead to a decline in ecosystem services, decreased carbon storage and mitigation of climate change. Furthermore, this study provides a first estimate for the growth rate of H. incrassata in the Mediterranean Sea, supporting evidence for the mechanism of its rapid extension.

Background: The hippocampus, entorhinal cortex (EC), and basal forebrain (BF) are among the earliest regions affected by Alzheimer’s disease (AD) pathology. They play an essential role in spatial pattern separation, a process critical for accurate discrimination between similar locations.Objective: We examined differences in spatial pattern separation performance between older adults with amnestic mild cognitive impairment (aMCI) with AD versus those with non-Alzheimer’s pathologic change (non-AD) and interrelations between volumes of the hippocampal, EC subregions and BF nuclei projecting to these subregions (medial septal nuclei and vertical limb of the diagonal band of Broca – Ch1-2 nuclei) with respect to performance.Methods: Hundred and eighteen older adults were recruited from the Czech Brain Aging Study. Participants with AD aMCI (n = 37), non-AD aMCI (n = 26), mild AD dementia (n = 26), and cognitively normal older adults (CN; n = 29) underwent spatial pattern separation testing, cognitive assessment and brain magnetic resonance imaging.Results: The AD aMCI group had less accurate spatial pattern separation performance than the non-AD aMCI (p = 0.039) and CN (p < 0.001) groups. The AD aMCI and non-AD groups did not differ in other cognitive tests. Decreased BF Ch1-2 volume was indirectly associated with worse performance through reduced hippocampal tail volume and reduced posteromedial EC and hippocampal tail or body volumes operating in serial.Conclusion: The study demonstrates that spatial pattern separation testing differentiates AD biomarker positive and negative older adults with aMCI and provides evidence that BF Ch1-2 nuclei influence spatial pattern separation through the posteromedial EC and the posterior hippocampus.

Objective: To assess current management of diverticulitis in Switzerland.Methods: Prospective observational study of diverticulitis management and outcomes in surgical departments over a 3-month time period. Hospital category was graded according to the Swiss Medical Association (FMH) as: U: University; A: Cantonal; B: Regional; P: Private.Results: 75 participating hospitals treated 1,015 patients, among whom 214 patients (21%) had elective sigmoid resections in 49 hospitals. Indication for elective resection were recurrent diverticulitis, previous complicated diverticulitis, fistulas, and stenosis. Surgeries were performed completely laparoscopically in 185 cases (86%) and required conversion to open in 19 cases (9%). Overall postoperative complication rate was 18% (n = 39) and no mortality was observed. Operation time, surgeons experience and hospital stay differed considerably between hospital categories.Conclusions: Elective sigmoid resection for diverticulitis in Switzerland was mainly performed laparoscopically with low postoperative morbidity. Different practices and outcomes between institutions were observed.

The lack of coordinated spectrum access for IoT wireless technologies in unlicensed bands creates inefficient spectrum usage and poses growing concerns in several IoT applications. Spectrum awareness becomes then crucial, especially in the presence of strict quality-of-service (QoS) requirements and mission-critical communication. In this work, we propose a lightweight spectral analysis framework designed for strongly resource-constrained devices, which are the norm in IoT deployments. The proposed solution enables model-based reconstruction of the spectrum of single radio-bursts entirely onboard without DFT processing. The spectrum sampling exploits pattern-based frequency sweeping, which enables the spectral analysis of short radio-bursts while minimizing the sampling error induced by non-ideal sensing hardware. We carry out an analysis of the properties of such sweeping patterns, derive useful theoretical error bounds, and explain how to design optimal patterns for radio front-ends with different characteristics. The experimental campaign shows that the proposed solution enables the estimation of central frequency, bandwidth, and spectral shape of signals at runtime by using a strongly hardware-limited radio platform. Finally, we test the potential of the proposed solution in combination with a proactive blacklisting scheme, allowing a substantial improvement in real-time QoS of a radio link under interference.

ABSTRACTBackground: The COVID-19 pandemic represents an unprecedented global challenge and implicates a wide range of burden on medical professionals. Here, we evaluated the perception of the COVID-19 pandemic among medical professionals in Germany.Methods: A total of n = 2827 medical professionals participated in an online survey between 27 March and 11 April.Results: While most participants stated that Germany was well prepared and rated the measures taken by their employer as positive, subgroup analyses revealed decisive differences. The preventive measures were rated significantly worse by nurses compared to doctors (p

Abstract In this work, we consider open-boundary conditions at high temperatures, as they can potentially be of help to measure the topological susceptibility. In particular, we measure the extent of the boundary effects at $$T=1.5T_c$$ T=1.5Tc and $$T=2.7T_c$$ T=2.7Tc . In the first case, it is larger than at $$T=0$$ T=0 while we find it to be smaller in the second case. The length of this “boundary zone” is controlled by the screening masses. We use this fact to measure the scalar and pseudo-scalar screening masses at these two temperatures. We observe a mass gap at $$T=1.5T_c$$ T=1.5Tc but not at $$T=2.7T_c$$ T=2.7Tc . Finally, we use our pseudo-scalar channel analysis to estimate the topological susceptibility. The results at $$T=1.5T_c$$ T=1.5Tc are in good agreement with the literature. At $$T=2.7T_c$$ T=2.7Tc , they appear to suffer from topological freezing, which prevents us from providing a precise determination of the topological susceptibility.

This contribution takes stock of ASEAN centrality in trade and the emerging policy area of trade infrastructure, also known as connectivity. ASEAN centrality in the East Asian and Indo-Pacific regions has increasingly been called into question, but most studies have failed to specify what ASEAN centrality is and how it can be measured. Outlining both a technical and a substantial definition, this study presents the state of affairs and current trends of ASEAN centrality in the areas of trade and connectivity. Disaggregating the concept, the paper assesses ASEAN’s role in the two policy areas as a leader, convener, convenience, and necessity. ASEAN’s central position in trade is under threat due to a changing environment, with trade ties increasing between ASEAN’s partners. In addition, ASEAN leadership in the RCEP negotiations has been symbolic rather than substantial. In connectivity, ASEAN centrality is even more questionable. Its regional connectivity vision is contested by other states and relationships act as conduits for the exercise of power.

Objectives: This study evaluated the impact of the interaction between the anorexigenic incretin hormone glucagon-like peptide-1 (GLP-1) and reward-related brain activity in the dorsolateral prefrontal cortex (DLPFC), a key area of behavioral control, on future weight loss in obese individuals. Methods: We performed a weight loss-weight maintenance intervention study over 27 months. We applied an fMRI food-cue reactivity paradigm during which the participants were passively exposed to food pictures to evaluate neuronal activity in the DLPFC. Additionally, we measured concentrations of circulating GLP-1 levels during a standard oral glucose tolerance test. Phenotyping was performed consecutively before and after a 3-month low-calorie diet as well as after a randomized 12-month trial, investigating the effect of a combined behavioral intervention on body weight maintenance. Participants were then followed-up for another 12 months without further intervention. Results: Using voxel-wise linear mixed-effects regression analyses, we evaluated 56 measurements and identified a strong interaction between circulating, endogenous GLP-1 levels and DLPFC activity predicting body weight change over the total observation period (t = −6.17, p = 1.6 · 10−7). While neither the GLP-1 nor the DLPFC response individually predicted the subsequent weight change, participants achieved body weight loss when the GLP-1 and the DLPFC responses occurred concurrently. Conclusions: Our data demonstrate an interaction between a peripheral hormonal signal and central nervous activity as robust predictor of body weight change throughout the different periods of a long-term life-style intervention. The preeminent role of their interdependency compared to the partly ambivalent effects of the single components argues for integrative approaches to improve sensitivity and reliability of weight prediction conventionally based on individual biomarkers. Keywords: Obesity, Body weight regulation, GLP-1, DLPFC, Food-cue reactivity, Voxel-wise linear mixed-effects regression

Abstract Background Club Cell protein (CC)16 correlates with lung injury and respiratory complications, which are in part triggered by polymorphonuclear leukocytes (PMNL) in severely traumatized patients (TP). CC16 exerts anti-inflammatory and immunosuppressive effects, however, its influence on PMNL functions after trauma is unknown. Here, we evaluated whether CC16 present in sera from TP could modify the biological functions of PMNL. Methods Sera from 16 severely injured TP without pneumonia (no P, n = 8) or with pneumonia (P, n = 8) were collected at admission to emergency department (ED) and 1 day prior pneumonia and pre-incubated with or without anti-CC16 antibody for CC16 neutralization. Samples from the equal post-injury days in the corresponding no P group were used. Neutrophils were isolated from healthy volunteers (HV, n = 5) and incubated with 20% of the serum medium from TP, respectively. In PMNL, CD62L, CD11b/CD18 and CD31 expression, migratory capacity, phagocytosis rate, oxidative burst and apoptosis were investigated. In isolated PMNL, CXCR1 and CXCR2 were neutralized before stimulation with CC16, and oxidative burst, phagocytosis and apoptosis were analyzed in neutrophils and their subsets. Results Serum from the P group enhanced significantly PMNL migration compared to no P group, while CC16-neutralization further increased the migratory rate of PMNL in both groups. CC16-neutralization increased significantly the expression of CD62L in the P group at ED. Oxidative burst was significantly increased in the P group vs. no P during the study period. CC16 seemed to have no influence on oxidative burst and phagocytosis in TP. However, in a more controlled study design, CC16 induced a significant increase of oxidative burst and a decrease of apoptosis of CD16+ granulocytes. These effects were markedly observed in mature CD16brightCD62Lbright and immune suppressive CD16brightCD62Ldim neutrophils. In mature subset, CXCR1 and CXCR2 neutralization diminished CC16-induced effects. Conclusions CC16 in sera from multiply traumatized patients, notably of those with pneumonia, has significant effects on PMNL. The results suggest an association of CC16 with CXCR1 and CXCR2. Our data suggest that CC16 reduces the migratory capacity of PMNL and thus modulates their function in patients with respiratory complications after trauma.

Abstract Background The yeast Pichia pastoris is a widely used host for the secretion of heterologous proteins. Despite being an efficient producer, we observed previously that certain recombinant proteins were mistargeted to the vacuole on their route to secretion. Simultaneous disruption of one vacuolar sorting pathway together with vacuolar proteases prevented this mis-sorting and resulted in higher levels of secreted heterologous protein. Inspired by the positive results, we now set out to investigate the influence of further parts of the vacuolar pathway, namely the Cvt-pathway and the homotypic fusion and protein sorting (HOPS) complex. Results Strains impaired in the Cvt pathway (∆atg11, ∆atg8) had no effect on secretion of the model protein carboxylesterase (CES), but resulted in lower secretion levels of the antibody fragment HyHEL-Fab. Disruption of genes involved in the HOPS complex led to vacuole-like compartments of the B category of vps mutants, which are characteristic for the deleted genes YPT7, VPS41 and VAM6. In particular ∆ypt7 and ∆vam6 strains showed an improvement in secreting the model proteins HyHEL-Fab and CES. Additional disruption of the vacuolar protease Pep4 and the potential protease Vps70 led to even further enhanced secretion in ∆ypt7 and ∆vam6 strains. Nevertheless, intracellular product accumulation was still observed. Therefore, the secretory route was strengthened by overexpression of early or late secretory genes in the vacuolar sorting mutants. Thereby, overexpression of Sbh1, a subunit of the ER translocation pore, significantly increased HyHEL-Fab secretion, leading up to fourfold higher extracellular Fab levels in the ∆ypt7 strain. The beneficial impact on protein secretion and the suitability of these strains for industrial applicability was confirmed in fed-batch cultivations. Conclusions Disruption of genes involved in the HOPS complex, especially YPT7, has a great influence on the secretion of the two different model proteins HyHEL-Fab and CES. Therefore, disruption of HOPS genes shows a high potential to increase secretion of other recombinant proteins as well. Secretion of HyHEL-Fab was further enhanced when overexpressing secretion enhancing factors. As the positive effect was also present in fed-batch cultivations, these modifications likely have promising industrial relevance.

Abstract Background The allotetraploid tobacco species Nicotiana benthamiana native to Australia has become a popular host for recombinant protein production. Although its usage grows every year, little is known on this plant’s genomic and transcriptomic features. Most N. benthamiana accessions currently used in research lack proper documentation of their breeding history and provenance. One of these, the glycoengineered N. benthamiana line ΔXT/FT is increasingly used for the production of biopharmaceutical proteins. Results Based on an existing draft assembly of the N. benthamiana genome we predict 50,516 protein –encoding genes (62,216 transcripts) supported by expression data derived from 2.35 billion mRNA-seq reads. Using single-copy core genes we show high completeness of the predicted gene set. We functionally annotate more than two thirds of the gene set through sequence homology to genes from other Nicotiana species. We demonstrate that the expression profiles from leaf tissue of ΔXT/FT and its wild type progenitor only show minimal differences. We identify the transgene insertion sites in ΔXT/FT and show that one of the transgenes was inserted inside another predicted gene that most likely lost its function upon insertion. Based on publicly available mRNA-seq data, we confirm that the N. benthamiana accessions used by different research institutions most likely derive from a single source. Conclusions This work provides gene annotation of the N. benthamiana genome, a genomic and transcriptomic characterization of a transgenic N. benthamiana line in comparison to its wild-type progenitor, and sheds light onto the relatedness of N. benthamiana accessions that are used in laboratories around the world.

Recent years brought many changes, which accelerated the development of different communication approaches and technologies. Nowadays, wireless technologies become the accelerators for a wide range of new applications. This paper focuses on the latest evolution of the promising wireless communication technology IQRF. For this aim, an in-depth analysis together with experimental measurements and simulations are provided. We provide original results in selected indoor and outdoor scenarios, in which the important communication parameters and technological limitations are highlighted. Last but not least, the application field of IQRF is established together with a comparison with other relevant technologies. DOI: 10.5755/j01.eie.25.2.22739

3D nanoprinting via focused electron beam induced deposition (FEBID) is applied for fabrication of all-metal nanoprobes for atomic force microscopy (AFM)-based electrical operation modes. The 3D tip concept is based on a hollow-cone (HC) design, with all-metal material properties and apex radii in the sub-10 nm regime to allow for high-resolution imaging during morphological imaging, conductive AFM (CAFM) and electrostatic force microscopy (EFM). The study starts with design aspects to motivate the proposed HC architecture, followed by detailed fabrication characterization to identify and optimize FEBID process parameters. To arrive at desired material properties, e-beam assisted purification in low-pressure water atmospheres was applied at room temperature, which enabled the removal of carbon impurities from as-deposited structures. The microstructure of final HCs was analyzed via scanning transmission electron microscopy—high-angle annular dark field (STEM-HAADF), whereas electrical and mechanical properties were investigated in situ using micromanipulators. Finally, AFM/EFM/CAFM measurements were performed in comparison to non-functional, high-resolution tips and commercially available electric probes. In essence, we demonstrate that the proposed all-metal HCs provide the resolution capabilities of the former, with the electric conductivity of the latter onboard, combining both assets in one design.

Rift Valley fever phlebovirus (RVFV) causes Rift Valley fever (RVF), an emerging zoonotic disease that causes abortion storms and high mortality rates in young ruminants as well as severe or even lethal complications in a subset of human patients. This study investigates the pathomechanism of intranuclear inclusion body formation in severe RVF in a mouse model. Liver samples from immunocompetent mice infected with virulent RVFV 35/74, and immunodeficient knockout mice that lack interferon type I receptor expression and were infected with attenuated RVFV MP12 were compared to livers from uninfected controls using histopathology and immunohistochemistry for RVFV nucleoprotein, non-structural protein S (NSs) and pro-apoptotic active caspase-3. Histopathology of the livers showed virus-induced, severe hepatic necrosis in both mouse strains. However, immunohistochemistry and immunofluorescence revealed eosinophilic, comma-shaped, intranuclear inclusions and an intranuclear (co-)localization of RVFV NSs and active caspase-3 only in 35/74-infected immunocompetent mice, but not in MP12-infected immunodeficient mice. These results suggest that intranuclear accumulation of RVFV 35/74 NSs is involved in nuclear translocation of active caspase-3, and that nuclear NSs and active caspase-3 are involved in the formation of the light microscopically visible inclusion bodies.

The receptor binding domain (RBD) of the SARS-CoV-2 spike protein plays a key role in the virus-host cell interaction, and viral infection. The RBD is a major target for neutralizing antibodies, whilst recombinant RBD is commonly used as an antigen in serological assays. Such assays are essential tools to gain control over the pandemic and detect the extent and durability of an immune response in infected or vaccinated populations. Transient expression in plants can contribute to the fast production of viral antigens, which are required by industry in high amounts. Whilst plant-produced RBDs are glycosylated, N-glycan modifications in plants differ from humans. This can give rise to the formation of carbohydrate epitopes that can be recognized by anti-carbohydrate antibodies present in human sera. For the performance of serological tests using plant-produced recombinant viral antigens, such cross-reactive carbohydrate determinants (CCDs) could result in false positives. Here, we transiently expressed an RBD variant in wild-type and glycoengineered Nicotiana benthamiana leaves and characterized the impact of different plant-specific N-glycans on RBD reactivity in serological assays. While the overall performance of the different RBD glycoforms was comparable to each other and to a human cell line produced RBD, there was a higher tendency toward false positive results with sera containing allergy-related CCD-antibodies when an RBD carrying β1,2-xylose and core α1,3-fucose was used. These rare events could be further minimized by pre-incubating sera from allergic individuals with a CCD-inhibitor. Thereby, false positive signals obtained from anti-CCD antibodies, could be reduced by 90%, on average.

Abstract This case of severe phenotype‐genotype mismatch brain tumor morphologically mimicking benign ganglioglioma emphasizes the urgent need for advanced molecular profiling in brain tumor diagnosis in the era of sophisticated molecular profiling.

Background: Yoga may pose a promising complementary therapy in the multimodal treatment of in-patients with schizophrenia spectrum disorders (SSD). However, to date, no studies have qualitatively examined in-patients' with SSD experiences of Yoga as well as their perceptions of its limitations and benefits as a treatment component. This qualitative study aimed to explore for the first time the mechanisms and processes of Yoga-based Group Intervention (YoGI) for in-patients with SSD in Germany by asking for their subjective experiences. Findings could serve as a preliminary basis for developing an effective and evidence-based YoGI manual tailored to this patient group.Materials and Methods: In total, 25 semi-structured interviews were conducted directly after YoGI, for which responses were either noted down by hand or audio-recorded. The interview guide was pilot-tested and consisted of 14 questions to explore the personal articulated experiences of participation in YoGI from in-patients with SSD. Positive, negative, depressive, and anxiety symptoms were assessed during a diagnostic interview and through questionnaires. The interview data was transcribed, coded by two independent researchers, and analysed using an inductive thematic approach. The research team collaboratively discussed emerging categories to reduce redundancy and form meaningful themes and subthemes.Results: The analysis revealed seven main themes. YoGI was perceived as feasible and focusing on individual adaptation, captured by the theme inclusivity. Nevertheless, participants encountered challenges; thus, physical limitations need to be considered. While practising together, participants experienced interconnectedness and developed a mindful stance as they accepted their limitations and adapted exercises with self-compassion. Patients described that following the flow of the asanas required physical persistence, which ultimately led many participants to experience confidence and relaxation. YoGI affected symptom representation as heightened awareness led participants to notice impeding as well as improved symptoms.Conclusion: YoGI showed various promising effects on in-patients with SSD. Future research should examine to what extent these effects can be sustained and how the mindful approach during YoGI can be transferred to areas outside the Yoga class. Furthermore, a randomised controlled trial could investigate the effectiveness of a manualised YoGI.

BackgroundEfficacy of vaccines and disease activity linked to immunization are major concerns among people with multiple sclerosis (pwMS).ObjectiveTo assess antibody responses to seasonal influenza antigens and vaccine-associated neuroaxonal damage utilizing serum neurofilament light chain (sNfL) in pwMS receiving dimethyl fumarate (DMF).MethodsIn this prospective study, the 2020/2021 seasonal tetravalent influenza vaccine was administered to 20 pwMS treated with DMF and 15 healthy controls (HCs). The primary endpoints were responder rate of strain-specific antibody production (seroconversion or significant (4-fold) increase in influenza-antibody titers for ≥2/4 strains) at 30 days post-vaccination and changes in sNfL levels.ResultsAll patients treated with DMF fulfilled the responder criteria for immunization compared with 53% of the controls. However, higher proportions of HCs already had influenza-antibody titers ≥1:40 at baseline (53% vs. 41%, p = 0.174). sNfL levels were comparable among both groups at baseline and did not increase 34 days after vaccination. In addition, no clinical or radiological disease reactivation was found.ConclusionDMF-treated patients mount an adequate humoral immune response to influenza vaccines. Within the limits of the small cohort investigated, our data suggest that influenza immunization is not associated with clinical or subclinical disease reactivation.

Metabolic endotoxemia has been suggested to play a role in the pathophysiology of metaflammation, insulin-resistance and ultimately type-2 diabetes mellitus (T2DM). The role of endogenous antimicrobial peptides (AMPs), such as the cathelicidin LL-37, in T2DM is unknown. We report here for the first time that patients with T2DM compared to healthy volunteers have elevated plasma levels of LL-37. In a reverse-translational approach, we have investigated the effects of the AMP, peptide 19-2.5, in a murine model of high-fat diet (HFD)-induced insulin-resistance, steatohepatitis and T2DM. HFD-fed mice for 12 weeks caused obesity, an impairment in glycemic regulations, hypercholesterolemia, microalbuminuria and steatohepatitis, all of which were attenuated by Peptide 19-2.5. The liver steatosis caused by feeding mice a HFD resulted in the activation of nuclear factor kappa light chain enhancer of activated B cells (NF-ĸB) (phosphorylation of inhibitor of kappa beta kinase (IKK)α/β, IκBα, translocation of p65 to the nucleus), expression of NF-ĸB-dependent protein inducible nitric oxide synthase (iNOS) and activation of the NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3) inflammasome, all of which were reduced by Peptide 19-2.5. Feeding mice, a HFD also resulted in an enhanced expression of the lipid scavenger receptor cluster of differentiation 36 (CD36) secondary to activation of extracellular signal-regulated kinases (ERK)1/2, both of which were abolished by Peptide 19-2.5. Taken together, these results demonstrate that the AMP, Peptide 19-2.5 reduces insulin-resistance, steatohepatitis and proteinuria. These effects are, at least in part, due to prevention of the expression of CD36 and may provide further evidence for a role of metabolic endotoxemia in the pathogenesis of metaflammation and ultimately T2DM. The observed increase in the levels of the endogenous AMP LL-37 in patients with T2DM may serve to limit the severity of the disease.

Nicotiana benthamiana is used worldwide as production host for recombinant proteins. Many recombinant proteins such as monoclonal antibodies, growth factors or viral antigens require posttranslational modifications like glycosylation for their function. Here, we transiently expressed different variants of the glycosylated receptor binding domain (RBD) from the SARS-CoV-2 spike protein in N. benthamiana. We characterized the impact of variations in RBD-length and posttranslational modifications on protein expression, yield and functionality. We found that a truncated RBD variant (RBD-215) consisting of amino acids Arg319-Leu533 can be efficiently expressed as a secreted soluble protein. Purified RBD-215 was mainly present as a monomer and showed binding to the conformation-dependent antibody CR3022, the cellular receptor angiotensin converting enzyme 2 (ACE2) and to antibodies present in convalescent sera. Expression of RBD-215 in glycoengineered ΔXT/FT plants resulted in the generation of complex N-glycans on both N-glycosylation sites. While site-directed mutagenesis showed that the N-glycans are important for proper RBD folding, differences in N-glycan processing had no effect on protein expression and function.