Search

• Rare arable plant cover can reduce the total number of aphids. • Cereal aphids decreased through predation of active hunting spiders. • Direct effects of rare arable plants only on active hunting spiders. In agricultural landscapes, arable plants are negatively affected by management intensification. These species can fulfill various ecosystem functions, such as biological pest control, by supporting predators. The ecosystem functions of common plant species are widely known. By contrast, the contribution of rare arable plants (RAPs) to biocontrol in cereal fields remains poorly understood. This study investigated the effect of RAPs on biocontrol potential. We compared cropped plots with and without sowing of rare and threatened arable plant species and investigated the effects on cereal aphids and their antagonistic predators, active hunting, and web-building spiders, as well as carnivorous/omnivorous carabids. We counted the total number of aphids on cereal shoots and trapped ground-dwelling arthropods on an experimental field and on 10 agricultural farms in the vicinity of Munich, Germany, in 2018 and 2019. The effects of the presence of RAPs were analyzed using linear mixed-effect models, whereas cover of RAPs was analyzed using structural equation models. Linear models revealed that the presence of RAPs did not significantly affect the aphid density and the activity densities of spiders and carabids. Structural equation models revealed direct negative effects of RAP cover on aphid density. However, no indirect effects via the predators of aphids were detected. Direct negative effects of active hunting spiders on aphids were determined, but not of the other potential predators. Our results suggest that RAPs may impact aphid infestation, however, the activity density of spiders and carabids were unsuitable indicators for such interactions. [ABSTRACT FROM AUTHOR]

Wetlands have an important ecological function and economic value. However, with economic development and urban expansion, wetland plants have suffered serious damage. Rare and endangered plants are "thermometers" that reflect the health processes of their ecosystems. To better protect the wetlands in Sanya, China, we systematically investigated and analyzed the species, quantities, distributions, and community characteristics of the rare and endangered plants in the Sanya River basin using the sample and sample strip methods. We established a total of 152 quadrats, of which 46 contained rare and endangered plants. We identified 27 rare and endangered plants that mainly appeared in the tree and shrub layers. The dominant families and genera of the community were evident. However, the proportion of families and genera with fewer or single species was high, indicating that the species composition of the community is complex, and the plant species diversity is rich. The dominant species in each layer of the community were evident, and the rare and endangered plants are occasional species of the community. The community similarity in the urban areas was high, indicating that the rare and endangered plants in these areas require highly homogenous habitats. The community similarity in the suburbs was low, indicating that the rare and endangered plants in these areas are highly adaptable to different habitats. Threat factors and vegetation coverage degree had a significant impact on the number of species and population size of rare and endangered plants. Finally, according to our study and IUCN classification criteria for the endangered levels, Sonneratia × gulngai meets the CR (Critical Endangered) assessment criteria, thus we recommend upgrading it to the endangered level from VU (Vulnerable). [ABSTRACT FROM AUTHOR]

Copyright of Fire Ecology is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Nepeta binaludensis Jamzad is an endemic and rare perennial plant belonging to the Lamiaceae family, which grows in a limited area in Binaloud Mountain in northeast of Iran. In this study to evaluate the diversity of 20 populations (localities) of N. binaludensis and the influence of environmental factors on essential oil (EO) content and composition, the plant aerial parts were collected at the full flowering stage. The plant materials dried in shade and subjected to hydro-distillation for obtaining their EOs. Analysis of the EO was carried out using GC and GC/MS. The oil yields were varied from 1.2 to 4.9%. Classification of populations was made based on EO compounds. The results of mean comparison between populations belong to different habitats showed that the populations of Darood and Friezy with average values of 4.91 and 1.2% had the highest and lowest EO yield, respectively. Twenty-two compounds were identified in the oils with 1,8-cineol (25.4–59.0%), 4aα,7α,7aα-nepetalactone (13.8–55.1%), myrcene (2.3–5.5%) and p-cymene (1.1–5.7%) as the main components. Result of correlation analysis showed that the oil yield was positively correlated with precipitation and negatively with temperature. In addition, 1,8-cineole was positively and nepetalactone was negatively correlated with altitude. Cluster analysis by Ward method categorized the populations into two groups. The major compound of the oils in cluster 1 was nepetalactone (with an average of 37.9%), while the oils in cluster 2, contained higher percentage of 1,8-cineole (52–59%). Most of the populations in cluster 2, were originated from high, cold, rainy, and steep areas. Highlights: By increasing the altitude, changes in growth and essential oil composition of the plant are observed Result of correlation analysis showed that essential oil yield was positively correlated with precipitation, and negatively with temperature. In the present study the populations with high-amount of nepetalactone and 1,8 cineole were identified, The populations of cluster 2 with higher amount of 1,8-cineole, were growing in the high altitude, cold, rainy and sloping areas [ABSTRACT FROM AUTHOR]

The ethnomedicinal application of rare and endangered medicinal plants holds significant promise for both traditional healing practices and modern healthcare advancements. This comprehensive review explores the multifaceted dimensions of this practice, emphasizing its ethnobotanical, conservation, pharmacological, cultural, and policy-related aspects. Drawing upon indigenous knowledge and traditional practices, the study underscores the importance of documenting and preserving ethanomedicinal wisdom, particularly concerning rare and endangered plant species. Conservation efforts emerge as a critical concern, as dwindling plant populations necessitate both in situ and ex situ strategies to safeguard these invaluable resources. Pharmacological investigations reveal the bioactive compounds present in these plants, supporting their potential for drug development and therapeutic innovations. Case studies spotlight specific ethnomedicinal plant species, exemplifying the delicate balance between traditional uses and contemporary conservation imperatives. Cultural and ethical considerations emerge as pivotal aspects, necessitating collaborative research models that respect indigenous knowledge and uphold intellectual property rights. The dynamic interplay between traditional practices and global market trends presents opportunities for sustainable livelihoods while posing challenges in maintaining equitable benefit-sharing. The legal and policy landscape is examined in light of international conventions, national legislation, and the evolving frameworks governing the utilization and protection of ethnomedicinal resources. As ethnomedicinal practices transition from local traditions to global enterprises, a harmonious coalescence of traditional wisdom and modern scientific rigor emerges as essential. This chapter offers insights into the current landscape and future prospects of ethnomedicinal practices involving rare and endangered medicinal plants. It underscores the urgency of collaboration between various stakeholders, bridging the gap between traditional knowledge and contemporary science for the preservation of biodiversity and the promotion of holistic healthcare systems. [ABSTRACT FROM AUTHOR]

Premise: Root‐sprouting (RS) is an evolutionarily independent alternative to axillary stem branching for a plant to attain its architecture. Root‐sprouting plants are better adapted to disturbance than non‐RS plants, and their vigor is frequently boosted by biomass removal. Nevertheless, RS plants are rarer than plants that are not root‐sprouters, possibly because they must overcome developmental barriers such as intrinsic phytohormonal balance or because RS ability is conditioned by injury to the plant body. The objective of this study was to identify whether phytohormones or injury enable RS. Methods: In a greenhouse experiment, growth variables, root respiration, and phytohormones were analyzed in two closely related clonal herbs that differ in RS ability (spontaneously RS Inula britannica and rhizomatous non‐RS I. salicina) with and without severe biomass removal. Results: As previously reported, I. britannica is a root‐sprouter, but injury did not boost its RS ability. Root respiration did not differ between the two species and decreased continuously with time irrespectively of injury, but their phytohormone profiles differed significantly. In RS species, the auxins‐to‐cytokinins ratio was low, and injury further decreased it. Conclusions: This first attempt to test drivers behind different plant growth forms suggests that intrinsic phytohormone regulation, especially the auxins‐to‐cytokinins ratio, might be behind RS ability. Injury, causing a phytohormonal imbalance, seems to be less important in spontaneously RS species than expected for RS species in general. [ABSTRACT FROM AUTHOR]

Berchemia racemosa Siebold & Zucc., 1845 is a rare species distributed in restricted areas in the western Korean peninsula. In this study, the complete chloroplast genome (plastome) of B. racemosa was sequenced and assembled by Illumina paired-end sequencing. The plastome of B. racemosa was 161,187 bp in length and was quadripartite in structure, including a large single-copy (LSC) region of 89,503 bp, a small single-copy (SSC) region of 18,214 bp, and two inverted repeats of 26,735 bp. The GC content was 37.2%. The plastome of B. racemosa contains 130 genes, including eight ribosomal RNA (rRNA) genes, 37 transfer RNA (tRNA) genes, and 85 protein-coding genes. Phylogenetic analysis using complete genome sequences showed that B. racemosa is most closely related to Berchemia flavescens. [ABSTRACT FROM AUTHOR]

The conservation actions of endangered plant species require a clear knowledge of their habitats. Urtica rupestris Guss. (Urticaceae) is a rare endemic plant species occurring on shady cliffs in the southern-eastern part of Sicily. In the last century, the extreme anthropogenic alterations of Hyblaean plateau have caused the continuous and unrestrained fragmentation of natural habitats and consequently the reduction and disappearance of some plant species. A total of 52 vegetation plots, of which 34 are unpublished, were analyzed in order to characterize the floristic composition of the U. rupestris community. All the relevés were classified using classification and ordination methods. The species is mainly linked to shady and wet rock habitats, and only secondarily colonizes the undergrowth shrubs. According to IUCN criteria, we propose a new risk status for this species and the establishment of a new habitat (92/43CEE) for correct long-term conservation. Finally, a new association, Urtico rupestris-Adiantetum capilli-veneris, which falls within the Polysticho setiferi-Phyllitidion scolopendrii alliance (Adiantetea capilli-veneris class), was described. This study can provide useful information for the management and conservation of U. rupestris. [ABSTRACT FROM AUTHOR]

This study was conducted to examine the effect of plant growth regulators on the rooting and growth of Rhododendron micranthum Turcz., a rare plant in South Korea. Indole-3-butyric acid (IBA) 150, 250, 350 mg·L−1 and α-naphthaleneacetic acid (NAA) 50, 150, 250 mg·L−1 treatment groups were established and cutting was performed in a commercial growth medium in March 2021 with 3 replications of 10 plants per group. After 70 days, the longest initial root growth was observed under the NAA 150 and 250 mg·L−1 treatments. Moreover, the number of roots and percentage of rooting were slightly higher in NAA 150 and 250 mg·L−1, indicating that these treatments were more effective in promoting plant growth. The above-ground part of plants treated with NAA 150 and 250 mg·L−1 had a taller plant height, longer and wider leaves, and a greater number of leaves compared to other groups. Accordingly, the survival rate of plants treated with NAA 150 and 250 mg·L−1 was higher than 80%, suggesting well-established roots and excellent above-ground growth. Therefore, treatment with growth regulator NAA at 150 and 250 mg·L−1 is considered beneficial for the cutting of R. micranthum Turcz. [ABSTRACT FROM AUTHOR]

Micropropagation is an effective way to preserve the gene pool of threatened plants. This study is devoted to the mathematical modeling of nutrient media and the study of the effect of mT (meta-topoline) on the multiplication of shoots of Hedysarum grandiflorum, Hyssopus cretaceus, and Matthiola fragrans in vitro in comparison with benzylaminopurine (BAP) and kinetin (KT). Initiation was performed on an MS medium with 0.5 mg/L BAP. For shoots multiplication, MS, B5, and WPM media were used with the addition of mT, BAP, KT. For H. grandiflorum, the multiplication coefficient of shoots was highest on medium B5 with the addition of mT at a concentration of 1 mg/L—2.90 shoots per plant, for H. cretaceus—B5 + 0.5 mg/L mT, and for M. fragrans—B5 + 1 mg/L KT. A positive effect of mT on H. grandiflorum and M. fragrans in vitro was found. The efficiency of using KT for H. cretaceus shoot multiplication is shown. The effectiveness of the B5 nutrient medium for H. grandiflorum and M. fragrans was determined. The positive effect of WPM for H. cretaceus micropropagation has been demonstrated. It is not recommended to use the MS media for micropropagation of these plant species. [ABSTRACT FROM AUTHOR]

Global warming is one of the most serious problems leading to changes in the distribution areas of species and biodiversity. Rhodiola quadrifida is a rare plant with adaptogenic properties and grows in the highlands in a narrow temperature range of 2–15 °C. The aim of our work was to study the growth and content of the main metabolites in two in vitro cultures of Rhodiola quadrifida at temperatures of 5, 15 and 25 °C. Hairy roots and calli were cultivated on agar medium for 28 days. The maximum values of the growth index were observed at 25 °C (2.32 and 2.12 for calli and hairy roots, respectively). HPLC-MS showed the absence of tyrosol and rosarin in both cultures, and rosin in the root culture. The content of salidroside changed slightly in calli and roots. Cultivation at 5 °C significantly stimulated the formation of rosin in calli. Only a residual amount of rosavin was noted in the roots, regardless of temperature. The content of rosin was higher in calli at 15 °C with a maximum content at the end of the cultivation cycle 25 µg/g DW. Thus, Rhodiola quadrifida will be able to grow with an increase in temperature by 10 °C but this will be accompanied by a significant reduction in its medicinal value [ABSTRACT FROM AUTHOR]

In a time of global change, having an understanding of the nature of biotic and abiotic factors that drive a species' range may be the sharpest tool in the toolbox of conservation and management of threatened species. However, such information are lacking for most tropical and epiphytic species due to the complexity of life history, the roles of stochastic events, and the diversity of habitat across the span of a distribution. In this study, we conducted repeated census across the core and peripheral range of Trichocentrum undulatum, a threatened orchid that is found throughout the island of Cuba (species core range) and southern Florida (the northern peripheral range). We used demographic matrix modeling and stochastic simulations to investigate the impacts of herbivory, hurricanes, and logging (in Cuba) on projected population growth rates (λ and λs) among sites. Our study indicates that the Florida population is under high extinction risk and most populations in the core range show less interannual variability, but, with the exception of one population, are also in decline. The peripheral population experienced higher herbivory damage than did the core populations, by a native inflorescence‐crippling fly, Melanagromyza miamensis, and an invasive, mortality‐triggered scale, Diaspis boisduvalii. The latter was found in the Florida population only. Hurricanes increased levels of mortality and had a negative effect on λ. However, λ increased following hurricane events due to increased recruitment and decreased herbivory. Life table response experiments suggest that higher adult survival was the largest contributor to the higher λ observed in the growing Cuba populations, as compared with the population in Florida. Logging simulations in Cuba indicate that populations can maintain stability only if selective logging of host trees occurs at low frequency. Reintroduction of the species in its northern peripheral habitat is needed to ameliorate the threats from sea‐level rise and invasive herbivorous insects to secure the current species' northern, continental range margin. Our study exhibits how vital rates and ecological interactions vary across a species distribution and respond under differing stochastic events. [ABSTRACT FROM AUTHOR]

Understory vascular plant species play a key role in natural ecosystems by maintaining nutrient balances and providing both food for wildlife and non-wood forest products. However, some such species are facing potential extirpation due to human interventions such as forest harvesting practices, land use change, and climate change. Despite their ecological and societal importance, the influence of different environmental variables on the distribution of vascular plant communities remains unclear. This study evaluated how several Acadian understory rare plant species (categorized as S1-S2 species) in New Brunswick will respond to future climate scenarios. We used presence-only occurrence records of four S1-S2 rare plant species and eight bioclimatic variables to calibrate climate suitability models using maximum entropy algorithm (MaxEnt). The key bioclimatic predictor variables for the two S1 species (Canada Honewort and Furbish Lousewort) were maximum temperature of the warmest month and precipitation of the driest quarter. The predictor variables with significant contribution for the two S2 plant species were annual precipitation (35%) for Anticosti Aster and precipitation of wettest quarter (85.7%) for Parker's Pipewort. Our models predicted that the two S1 plant species are likely to gain more suitable climate space by 2070, while the two S2 plant species are likely to lose suitable climate space and may face extirpation risk from New Brunswick. The difficulty of projecting rare plant species is very scarce occurrence data, but analyses such as ours can help indicate current locations and in-situ and ex-situ climate change refugia areas for use in biodiversity conservation planning. [ABSTRACT FROM AUTHOR]

Abstract—The floristic complexes of the Vetlyanka River are characterized by a high pool of rare plants with various protection categories in the Saratov region. In total, the complexes include six species: Bulbocodium versicolor, Hyacinthella leucophaea, Tulipa gesneriana, Iris pumila, I. halophila, and Scilla siberica. These ecotopes have been recorded for the first time for S. siberica, B. versicolor, and Iris halophila. At the same time, S. siberica and I. halophila have a narrow ecological range. The studied cenopopulations of rare plants in the floristic complexes of the Vetlyanka River are stable in time and space. There is a self-renewal process in cenopopulations of the studied plants, which is hampered by a number of anthropogenic factors. The ontogenetic spectrum of the B. versicolor cenopopulation is a single-vertex spectrum with a high proportion of individuals of the generative fraction (70.2%). The age spectrum of the S. siberica cenopopulation is dominated by pregenerative plants (67.4% of the total number of the individuals studied) with the maximum number recorded for immature individuals (46.5%). The high proportion of pregenerative plants (52.5%) in the T. gesneriana cenopopulation indicates its capability for self-renewal and is characterized by a bimodal age spectrum. According to the classification by T.A. Rabotnov, the cenopopulations of the studied species are normal incomplete. [ABSTRACT FROM AUTHOR]

Microbes influence plant growth and fitness. However, the structure and function of microbiomes associated with rare and endemic plants remain underexplored. To investigate the bacterial community structure of Ulleung-sanmaneul (U-SMN), an endemic plant in Korea, samples were collected from natural and cultivated habitats, and their 16S rDNA was sequenced. The root bacterial community structure differed from those of bulk soil and rhizosphere in both habitats. Endogenous bacteria in cultivated plants were less diverse than wild plants, but Luteibacter rhizovicinus, Pseudomonas fulva, and Sphingomonas pruni were shared. Co-inoculation of Pseudoxanthomonas sp. JBCE485 and Variovorax paradoxus JBCE486 promoted growth and induced salt stress resistance in Arabidopsis and chive. Changes in growth promotion and phenotypes of plants by co-inoculation were mediated by increased auxin production. Each strain colonized the roots without niche competition. The results indicated that host selectivity was influential than environmental factors in formulating endophytic bacterial composition, and domestication simplified the bacterial community diversity. Our results will contribute to the growth and maintenance of endemic U-SMN plants. [ABSTRACT FROM AUTHOR]

Areas of endemism are a standard unit of biogeography and are commonly used for analytical biogeographic analyses as a criterion to identify areas for conservation. Galianthe is a genus of 56 species of mainly herbaceous plants from the family Rubiaceae found in the Neotropics, occurring in campos rupestres (quartzitic rock outcrops), cerrado (savanna), lowland grasslands, campos de altitude (highland grasslands), sasonally dry tropical forests (caatinga vegetation), and savanna hillsides. Currently, the conservation status assessment is only known for eleven species of Galianthe, and there have been no previous attempts to identify areas of endemism for the genus. In view of this, we used the NDM/VNDM software to analyze the distribution of the 56 species of Galianthe to identify areas of endemism and to recognize which species define each area. We also evaluated the conservation status of forty-five species for first time, according to the International Union for Conservation of Nature (IUCN) guidelines. Our finding identified 12 areas of endemism with three cell sizes, mainly in the eastern part of Paraguay and southern Brazil. Illustrating the general biodiversity crisis, 41% of the Galianthe species (23 spp.), were evaluated as Endangered due to their degree of endemism and destruction of their habitat, while 31 species were evaluated as not threatened and two species had Insufficient Data. In turn, only five threatened species were found within or on the periphery of protected areas. We consider that although protected areas are designed to protect biodiversity, in recent decades they have experienced different degrees of threat. Due to the high risk of many species of the genus, it is necessary to consider restoration (as a complement to conservation) of the processes that maintain grasslands to conserve unique, endemic and threatened species. [ABSTRACT FROM AUTHOR]

Many organisms change their phenotype in response to the environment, a phenomenon called phenotypic plasticity. Although plasticity can dramatically change the phenotype of an organism, we hardly understand how this can affect biotic interactions and the resulting phenotypic selection. Here, we use fast cycling Brassica rapa plants in an experiment in the greenhouse to study the link between plasticity and selection. We detected strong plasticity in morphology, nectar, and floral scent in response to different soil types and aphid herbivory. We found positive selection on nectar and morphological traits in hand‐ and bumblebee‐pollinated plants. Bumblebee‐mediated selection on a principal component representing plant height, flower number, and flowering time (mPC3) differed depending on soil type and herbivory. For plants growing in richer soil, selection was stronger in the absence of herbivores, whereas for plants growing in poorer soil selection was stronger with herbivory. We showed that bumblebees visited tall plants with many flowers overproportionally in plants in poor soil with herbivory (i.e., when tall plants were rare), thus causing stronger positive selection on this trait combination. We suggest that with strong plasticity under most stressful conditions, pollinator‐mediated selection may promote adaptation to local environmental factors given sufficient heritability of the traits under selection. [ABSTRACT FROM AUTHOR]

Background and aims: The “ombusillo”, Phytolacca tetramera, is a rare plant endemic to the northeast of Buenos Aires province, Argentina. The objective of this work is to provide a more accurate approximation of its distribution area, to evaluate the conservation status of the species and to list the threats to its conservation. M&M: The occurrence sites were obtained from herbarium specimens, databases, bibliography, and fieldwork. We delimited geographic cores, or potential subpopulations, for groups of “ombusillos”. We surveyed the activities that threaten the survival of the species and finally the conservation status was evaluated using the IUCN criterion B1 (extent of occurrence). Results: Phytolacca tetramera is currently restricted to the coast of the Río de la Plata between 4 and 15 m asl, from the district of La Plata to the Samborombón Bay. It has an extension of presence of 4313.714 km2, in which four geographic cores were identified. In accordance with IUCN criterion B1, we propose the category Endangered B1ab(i,iii) for P. tetramera. The threats to the survival of the species are diverse and need further studies, urbanization being one of the most serious. Conclusions: Due to the restricted geographical extension of P. tetramera and its possible threats, it is essential to carry out actions for the conservation of this species. Among the priority actions for the future, it is essential to know more about its dispersal capacity and regeneration niche. [ABSTRACT FROM AUTHOR]

To select genotypes with stable growth in diverse environments, tree breeders use multisite trials to evaluate genotypic stability and adaptation. Since genotype-by-environment (G × E) interaction effects vary with age, most multisite trials focus only on site effects, ignoring age effects. Liriodendron tulipifera trees are valuable due to their rapid growth and high-quality wood. Currently, multisite trials involving L. tulipifera plants are rare and the lack of data on G × E interaction effects impedes its selection. In this study, to explore the better performance and G × E pattern of L. tulipifera across ages, the growth traits (tree height, H; and diameter at breast height, DBH) of trees that were of five consecutive ages and grown on progeny-testing plantations were studied for 27 open-pollinated families at three sites. The results showed that the heritability of DBH was greater than that of H at almost all ages, and the individual breeding value ranking differed across sites and ages. The additive genetic correlations (rA) between different site pairs were relatively small and varied with age, indicating an age trend for G × E, and showed a difference in traits. It was found that the absolute differences in some monthly average climatic indicators correlated with the G × E. Based on a comprehensive analysis considering stability and productivity, four elite families were identified. These results could aid in selecting stable, adaptable L. tulipifera genotypes and provide a reference for evaluating G × E interaction effects in multiage, multisite trials of other tree species. [ABSTRACT FROM AUTHOR]

A recent study has found that the decline of seed dispersers, such as birds, mammals, reptiles, and ants, in Europe could pose a threat to one-third of plant species. Seed dispersal plays a crucial role in maintaining ecosystem health and allows plants to adapt to climate change and recover after wildfires. The study compiled data on over 5000 pairings of plants and their animal dispersers, revealing that more than one-third of seed-dispersing animal species in Europe are either threatened or declining. The decline of these seed dispersers could have significant consequences for plant species and ecosystems. [Extracted from the article]

A recent study published in Science reveals that the decline of seed-dispersing animals in Europe is threatening the survival of at least one-third of plant species. The loss of these animals, including birds, mammals, reptiles, and ants, could hinder the ability of plants to adapt to climate change or recover after wildfires. The study, which analyzed thousands of species interactions, found that more than one-third of seed-dispersing animal species in Europe are either threatened or declining. The researchers also identified several plant-animal interactions that are of "very high concern" due to the endangered status of both the plant and the animal involved. However, there is still much to learn about the extent of this problem and its impact on ecosystems. [Extracted from the article]

We present the first empirical information on floral traits, floral visitors, and breeding system of Anarthrophyllum strigulipetalum, a rare and charismatic plant species that inhabits rocky terrains in the harsh semi-arid landscapes of northwestern Patagonia. Despite being large plants (∼1.7 m2 surface area), with a showy floral display (>100,000 flowers/plant), protracted flowering period (∼1 month), high nectar production (16.7 μl per flower), high pollen viability (96%) and pollen receipt (∼200 grains/stigma), species reproductive success was very low, with less than 1% of the flowers developing into fruit. Only birds triggered the flower-opening mechanism and carried pollen, whereas insects behaved as nectar thieves. The addition of nectar seemed to increase fruit formation compared to control flowers, whereas nectar removal tended to decrease fruit set. The natural low fruiting levels could be the result of a combination of factors such as low legitimate pollinator visitation rate, low-diverse and poor-quality pollen receipt due to geitonogamy promoted by the great floral display and the abundant floral reward, intense levels of nectar theft by native insects, and even climatic stress. The reduced size of populations and low fitness settles the grounds for potentially low genetic diversity, inbreeding depression and, ultimately, demographic declines. • Anarthrophyllum strigulipetalum is a rare plant that inhabits semi-arid Patagonia. • Reproductive strategies of plants under adverse climatic conditions. • Improve understanding of rare species and their persistence in harsh environments. • Large floral display and abundant reward can promote high levels of geitonogamy. • Low fruiting owing pollination deficit, geitonogamy, nectar theft and climatic stress. [ABSTRACT FROM AUTHOR]

Since 1988, the Cooperative Endangered Species Conservation Fund or “Section 6” fund facilitates partnerships between the U.S. Fish and Wildlife Service and state agencies that aim to provide data pertinent to the recovery of Endangered Species Act (ESA) protected species. Despite the success of these efforts, research for rare plants is chronically underfunded and many species experience long periods of research inactivity that hinders their conservation. One example is Macbridea alba Chapm. (white birds-in-a-nest, Lamiaceae, M. alba from hereon), a federally threatened and state endangered mint endemic to four counties within the Florida panhandle. The species is a candidate for delisting after 30 years of protection under the ESA, however a lack of up-to-date data associated with the species has continually challenged the implementation of effective conservation programs and prolonged the recovery process. The focus of this paper is to review the timeline of recovery goals for M. alba, present a summary of recent research findings (i.e., species distribution models, habitat associations, reproductive ecology), and identify achievements as well as persistent obstacles to recovery and delisting. Our research focused on 5 of 10 recovery actions listed in the recovery plan for M. alba. Our findings provide updated data and make novel contributions to the protection of M. alba that will prioritize and improve management efforts. Overall, our work highlights frequent barriers to the recovery and delisting of rare species, using an endemic plant species as a case-study. Importantly, we outline effective methods for the rapid assessment of at-risk plant species that due to enduring data gaps, face an uncertain future in listing and recovery. We hope our work provides a convincing case demonstrating the critical need for current and expanded ESA funding and encourages a diversity of individuals and institutions to participate in critical rare plant research to swiftly fill research gaps and expedite recovery of some of the rarest plant species across the United States.

Abstract In a time of global change, having an understanding of the nature of biotic and abiotic factors that drive a species' range may be the sharpest tool in the toolbox of conservation and management of threatened species. However, such information are lacking for most tropical and epiphytic species due to the complexity of life history, the roles of stochastic events, and the diversity of habitat across the span of a distribution. In this study, we conducted repeated census across the core and peripheral range of Trichocentrum undulatum, a threatened orchid that is found throughout the island of Cuba (species core range) and southern Florida (the northern peripheral range). We used demographic matrix modeling and stochastic simulations to investigate the impacts of herbivory, hurricanes, and logging (in Cuba) on projected population growth rates (λ and λs) among sites. Our study indicates that the Florida population is under high extinction risk and most populations in the core range show less interannual variability, but, with the exception of one population, are also in decline. The peripheral population experienced higher herbivory damage than did the core populations, by a native inflorescence‐crippling fly, Melanagromyza miamensis, and an invasive, mortality‐triggered scale, Diaspis boisduvalii. The latter was found in the Florida population only. Hurricanes increased levels of mortality and had a negative effect on λ. However, λ increased following hurricane events due to increased recruitment and decreased herbivory. Life table response experiments suggest that higher adult survival was the largest contributor to the higher λ observed in the growing Cuba populations, as compared with the population in Florida. Logging simulations in Cuba indicate that populations can maintain stability only if selective logging of host trees occurs at low frequency. Reintroduction of the species in its northern peripheral habitat is needed to ameliorate the threats from sea‐level rise and invasive herbivorous insects to secure the current species' northern, continental range margin. Our study exhibits how vital rates and ecological interactions vary across a species distribution and respond under differing stochastic events.

In 2020, a new site of the ghost orchid (Epipogium aphyllum Swartz) was found in the area of Mt. Jadovnik, near Drvar in the western part of Bosnia and Herzegovina. This mycoheterotrophic orchid belongs to one of the rarest and least known species of orchids in the world. The paper gives a brief description of the ecology of the species, a description of the habitat conditions in the site area, and an overview of the previous records for Bosnia and Herzegovina. It was found in beech and fir dominated primeval forest, in Visućka draga on Mt. Jadovnik. So far, it has been confirmed in only a few locations in Bosnia and Herzegovina.

• Seed germination rate of lachenalia viridiflora is of 60%. • Most often, bulbs reach the ability to flower in the third season after sowing the seeds. • The results refer to the problem of endangered plant species native to south africa. Generative propagation is widely used for the conservation of endangered plant species. The aim of this study was to trace the course of endangered and ornamental Lachenalia viridiflora development. Seeds were sown, and the obtained bulbs were used for observation in subsequent growing seasons until flowering plants were obtained. First seedlings occurred after 3 weeks and finally 61% of seeds germinated and formed bulbs in the sowing season. 20% of these bulbs were able to flower in 2nd but most bulbs flowered in the 3rd year after sowing. Morphological attributes (leaves, inflorescences and bulbs parameters) were monitored throughout the years of the ongoing experiment. The presented results fit into the issue of protecting of rare plant species and attempts to reintroduce them to the natural environment. [ABSTRACT FROM AUTHOR]

Rare plant species that are constrained by shading may be threatened by a lack of natural disturbance that removes overhanging vegetation. The original distribution of the study species Physostegia correllii (Lundell) Shinners included freshwater floodplains of large rivers in the southcentral U.S. (Colorado, Rio Grande, and Mississippi rivers). A second species, Trillium texanum Buckley was found in seep spring baygalls in east-central Texas and extreme northwestern Louisiana. Experiments to determine the effects of shading on P. correllii and T. texanum were conducted using short-term shade cloth treatments (full sunlight vs. 30% shading for 2-3 weeks), and a dryness treatment for T. texanum (moist vs. less moist). Mean height and cover responses of individuals for both species were determined in conservation gardens located in Lafayette, Louisiana. Physostegia correllii grown in shaded environments for 2.5 weeks had shorter mean height than if grown in full sunlight. Half of the shaded plants in shaded plots had died by the mid-summer. For T. texanum, shading reduced the mean height and cover of plants. Therefore, management to remove overhanging ground vegetation to mimic natural disturbance might revive P. correllii and/or T. texanum populations where overhanging vegetation is increasing due to lack of natural disturbance (e.g., flood pulsing, grazing, burning). [ABSTRACT FROM AUTHOR]

Rational re‐design of the substrate pocket of phenylpropanoid‐flavonoid O‐methyltransferase (PFOMT) from Mesembryanthemum crystallinum, an enzyme that selectively methylates the 3′‐position (= meta‐position) in catechol‐moieties of flavonoids to guiacol‐moieties, provided the basis for the generation of variants with opposite, i. e. 4′‐ (para‐) regioselectivity and enhanced catalytic efficiency. A double variant (Y51R/N202W) identified through a newly developed colorimetric assay efficiently modified the para‐position in flavanone and flavanonol substrates, providing access to the sweetener molecule hesperetin and other rare plant flavonoids having an isovanilloid motif. [ABSTRACT FROM AUTHOR]

A lobed leaf is a common trait in plants, but it is very rare in Lauraceae plants, including species of Phoebe. In the study of germplasm resources of Phoebe neurantha, we found lobed leaf variant seedlings, and the variation could be inherited stably. Studying the lobed leaf mechanism of P. neurantha var. lobophylla can offer insight into the leaf development mechanism of woody plants. RNA-seq and small RNA-seq analysis results showed that a total of 8091 differentially expressed genes (DEGs) and 16 differentially expressed miRNAs were identified in P. neurantha var. lobophylla. Considering previous research results, a leaf margin morphological development related miRNA, pne-miRNA319a, was primary identified as a candidate miRNA. Target gene prediction showed that a total of 2070 genes were predicted to be the target genes of differentially expressed miRNAs. GO enrichment analysis of differentially expressed target genes suggested that PnTCP2 is related to lobed leaf formation. The TRV-VIGS gene silencing of PnTCP2 led to lobed leaves in P. neurantha seedlings. The downregulation of PnTCP2 led to lobed leaves. The yeast two-hybrid test and bimolecular fluorescence complementation test confirmed that the PnTCP2 protein interacted with the PnLBD41 protein. Based on the expression analysis of gene-silenced leaves and RNA-seq and small RNA-seq analysis results, pne- miRNA319a and PnLBD41 might also play important roles in this process. In conclusion, PnTCP2 plays an important and vital role in the formation of the lobed leaves of P. neurantha var. lobophylla. [ABSTRACT FROM AUTHOR]

Artemisia simplex (Laramie chickensage) is an imperiled, endemic plant in southeastern Wyoming, USA. To fill critical information gaps, we measured its seed viability and pollination mechanisms, measures that are lacking for many rare and endemic plants yet critical for the perpetuation of species. Artemisia simplex cover varied among sites and occupied 5.5% (range: 1.3–14.6%) of the area on average. This unique plant typically produced viable seeds under ambient conditions but did not self‐pollinate. Characteristics of the flower and pollen suggest that A. simplex is primarily animal‐pollinated, and we found that both wind and animals transported pollen to the plant, but wind alone produced fewer seeds. Members of the sagebrush genus are widely known to be wind‐pollinated; however, we demonstrated that this species of sagebrush is largely animal‐pollinated. Plant conservation and management plans must consider the mode of pollen transfer, especially when considering the decline of insect pollinators. Pollination provides information about the persistence of plant populations through sexual reproduction, which produces the genetic variation needed for healthy populations. [ABSTRACT FROM AUTHOR]

The Zamin State Reserve, located on the western spurs of the Turkestan Range in the upper reaches of the Sangzar River, is the richest region of Uzbekistan in floristic and phytocenotic terms. This reserve, established in 1926, is unique in the number of rare endangered plant species growing in this small area. Juniper forests, in their completeness and richness of tree and shrub species, have no analogues in our republic. At least 20 plant species have been described from this territory, which is comparable to the most famous flora of Chimgan. List of vascular plants compiled and published by M.G. Popov, N.V. Androsov in 1937, for a long time was almost the only floristic information throughout the Western Pamirs. In the second half of the 20th century, large-scale floristic studies were carried out not only in this region, but also throughout the territory of Uzbekistan. In particular, lists of floras of the Chatkal, Nurata and Surkhan reserves were published. Thus, the dendroflora studied by us, richer in the number of plant species, will be a good help for creating an electronic database of the modern flora of Uzbekistan. [ABSTRACT FROM AUTHOR]

Alpinia malaccensis (Burm.f.) Roscoe is a rare plant in Java, Indonesia, that produces essential oils. Its sustained decline causes great concern for future uses. Therefore, the study of its diversity in its natural habitat to predict future survival needs serious focus. The present study aims to determine the genetic diversity of A. malaccensis in Java based on ISSR markers. The sampling of a total of 11 populations yielded 33 accessions, which underwent amplifying using 12 ISSR primers. Using the molecular data analyzed their relationships, population structure, and genetic diversity. The UPGMA cluster, structure, and principal component analysis (PCA) demonstrated that the accessions divide in three groups, a result that correlates with their geography in Java. Group I consisted of var. malaccensis populations, while groups II and III comprised the var. nobilis populations, which proved truer among populations of var. nobilis. The genetic diversity category of A. malaccensis showed moderate based on Nei's genetic diversity (h = 0.2892) and Shannon's information index (I = 0.4438). The coefficient of genetic differentiation (GST) and molecular variance among the populations displayed higher within the individual population, which revealed a significant genetic variance among the 11 populations of A. malaccensis. The latest results confirm that ISSR markers can help distinguish the genotypes of A. malaccensis. [ABSTRACT FROM AUTHOR]

Rare species serve important ecosystem functions, including community resilience to global change. Yet rare species are disappearing globally because of anthropogenic activities such as fire suppression. Prescribed burning is a widespread management approach that can reduce invasive plant presence, recycle nutrients, and restore species diversity. However, the effects of prescribed burning on rare plants are not well understood. We analyzed the population dynamics of 67 rare, native plant species in response to prescribed burning using the Chicago botanic garden's Plants of Concern dataset. This dataset includes rare plant populations concentrated in the northeast part of Illinois, and a few populations in Indiana and Wisconsin, United States. We evaluated the effects of burning by comparing the percent change in population size in the short‐term (1–2 years) and long‐term (3–4 years) after prescribed burning, to the percent change in population size not following burns. In a global model with all species, we did not detect the effects of burning on percent change in population size. In species‐level analyses, we detected burn effects for most species for which we had the statistical power to detect possible burn responses, although the direction of their responses was mixed. Five species responded consistently between short‐ and long‐term datasets, and four species had mixed responses, with most responding positively over the short‐term and negatively over the long‐term. We complemented this analysis with a literature review of fire responses for available species. Our literature review revealed more responses to burning than what we found from our analyses; however, most of this evidence does not compare burned and unburned populations directly and should be treated cautiously. Through community science monitoring efforts, we were able to compile one of the largest studies of burn effects on rare plants to date, but continued monitoring is necessary to fully evaluate how prescribed burning impacts rare plant species. [ABSTRACT FROM AUTHOR]

Ferula sinkiangensis is a perennial herb, producing fruits only once through its life history, and exclusively habitated in Xinjiang being in the list of state rare and endangered plants Level 2. Besides andromonoecy, usually found in Umbelliferae, plants of male sterility were observed in their populations. Aimed to male sterility and the factors inducing it, comparative observation on morphology of male sterility individuals and functional female flowers were executed through field investigation, and study on cytology of the process of the anther degeneration in female flowers were made from paraffin sections. The results were as follows:(1)Female plants germinated at the end of March and blossomed in the middle of April, and their fruits matured at the end of May; Phenological phases of them were the same as those of andromonoecy.(2)The average height and diameter of plant, and number of the first level branch of female individuals were(71.00±10.92)cm,(71.67±17.64)cm, and(23.83±2.04), separately; The average length and width of basal leaves were(33.41±11.63)cm and(24.47±8.60)cm; The female and the andromonoecy were the same in size of both the plant and the leaf.(3)On female plants, inflorescences on the first and the second level branches were all female, which could bear fruits wholly; The number of umbels in the inflorescence(13.22±4.70)and number of flowers in the umbel(12.03±2.30)and number of total flowers in the inflorescence(159.08)were higher than those in individuals of hermaphrodite; Female plants produced more flowers which could set fruits and bore out-crossing seeds of heterosis.(4)In female umbels flowers located tightly and the distance among flowers were shorter than those in both hermaphrodite and male umbels; Female flowers opened slightly in the whole flowering stage, while petals of both hermaphrodite and male flowers folded reversely.(5)There were no differences in morphological characteristics of petals and pistils among different sexual flowers. But the length(1.79±0.39 mm)and width(1.10±0.21 mm)of petals, and the length of stamens(0.6-1.3 mm)in the functional female flower were the smallest, where anthers degenerated and no pollen stain well developed.(6)Abnormal development of the anther on the degenerated stamen appeared in both of parietal cells and male gametophytes from sporogenous cell stage to microspore tetrad stage. In conclusion, the nutrients productivity in plants of male sterility is similar as andromonoecy, but patterns of resource distribution are quite different; there are significant differences between female and hermaphrodite/male flowers in both the morphology and the function; abnormal development of parietal cells, especially because of the lack of tapetum, caused abortion of microspores in the functional female flower. [ABSTRACT FROM AUTHOR]

Two new tetraploid quillwort species, Isoëtes longpingii and I. xiangfei, from China are described and illustrated. I. longpingii is a fully submerged plant found in a small pond of Hunan Province. It is morphologically similar to I. sinensis, but differs in its small and tuberculate, cristate megaspores and soft slender leaves that grow up to 60 cm. It is also similar to hexaploid I. orientalis, but differs in its 44 chromosomes and tuberculate, cristate megaspores. I. xiangfei is most similar to diploid I. yunguiensis in megaspore ornamentation, but differs in its microspore ornamentation, oblong sporangium, and 44 number of chromosomes. There are a few individuals of I. longpingii found in Ningxiang City of Hunan Province, and I. xiangfei is distributed in the wetlands of Tongdao and Huitong counties of Hunan Province. Because of their limited geographic ranges, small populations, fewer individuals and disturbed habitats, I. longpingii and I. xiangfei are evaluated as critically endangered (CR) and vulnerable (VU) category, respectively, according to the IUCN Red List criteria. A key to all the current known Chinese quillworts is also provided for further taxonomic identification and conservation of these rare and endangered plants in China. [ABSTRACT FROM AUTHOR]

Restoration ecology commonly seeks to re‐establish species of interest in degraded habitats. Despite a rich understanding of how succession influences re‐establishment, there are several outstanding questions that remain unaddressed: are short‐term abundances sufficient to determine long‐term re‐establishment success, and what factors contribute to unpredictable restorations outcomes? In other words, when restoration fails, is it because the restored habitat is substandard, because of strong competition with invasive species, or alternatively due to changing environmental conditions that would equally impact established populations? Here, we re‐purpose tools developed from modern coexistence theory to address these questions, and apply them to an effort to restore the endangered Contra Costa goldfields (Lasthenia conjugens) in constructed ("restored") California vernal pools. Using 16 years of data, we construct a population model of L. conjugens, a species of conservation concern due primarily to habitat loss and invasion of exotic grasses. We show that initial, short‐term appearances of restoration success from population abundances is misleading, as year‐to‐year fluctuations cause long‐term population growth rates to fall below zero. The failure of constructed pools is driven by lower maximum growth rates compared with reference ("natural") pools, coupled with a stronger negative sensitivity to annual fluctuations in abiotic conditions that yield decreased maximum growth rates. Nonetheless, our modeling shows that fluctuations in competition (mainly with exotic grasses) benefit L. conjugens through periods of competitive release, especially in constructed pools of intermediate pool depth. We therefore show how reductions in invasives and seed addition in pools of particular depths could change the outcome of restoration for L. conjugens. By applying a largely theoretical framework to the urgent goal of ecological restoration, our study provides a blueprint for predicting restoration success, and identifies future actions to reverse species loss. [ABSTRACT FROM AUTHOR]

The response of Darlingtonia californica Torr. on the effect of Cu, Co, and Mg ions in vitro is studied. The following concentrations of mineral salts are tested: 0.1 (control), 10, 25, 50, 75, and 100 μM CuSO4·5H2O (which corresponds to 0.006, 0.6, 1.6, 3.2, 4.8, and 6.4 mg/L of water-soluble Cu ions); 0.1 (control), 10, 25, 50, 75, and 100 μM CoCl2·6H2O (corresponding to 0.006, 0.6, 1.5, 3.0, 4.4, and 5.9 mg/L of water-soluble Co ions); and 1500 (control), 4500, 7500, 10500, and 15 000 μM MgSO4·7H2O (corresponding to 36, 108, 181, 253, and 362 mg/L of water-soluble Mg ions). The results show a direct significant correlation between the accumulation of Cu, Co, and Mg ions in D. californica plants depending on their content in nutrient solutions. The maximum accumulation of ions is found after 45 days of cultivation, Cu—138.24 mg/kg of dry matter, Co—249.92 mg/kg, and Mg—4722.26 mg/kg. It is shown that the concentrations used do not significantly affect the parameters of plant growth and development in vitro. Morphological and anatomical studies confirm the high level of ecological adaptation of this species to extreme conditions. In addition, the plants do not require specific cultural conditions; they are able to grow on nutrient media rich in mineral composition at a temperature of 24 ± 1°C and do not require low positive temperatures for the root system. The results show that D. californica plants are able to accumulate Cu, Co, and Mg ions and tolerate high concentrations of these elements in nutrient media in vitro, but they are not characterized by an increased need for these elements for normal growth and development. [ABSTRACT FROM AUTHOR]

Copyright of ACTA Scientiarum Polonorum Silvarum Colendarum Ratio et Industria Lignaria is the property of Poznan University of Life Sciences Press and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

This document provides information on the discovery of a new natural community called the button sedge fen in New Hampshire. The fen is characterized by the dominant presence of Carex bullata (button sedge) and specific environmental patterns. The document describes the vegetation, soil composition, and water pH of the fen, and highlights its rarity and conservation status. It also discusses related communities in the peatlands and emphasizes the importance of conservation efforts to protect these unique ecosystems. [Extracted from the article]

Premise: The effective ex situ conservation of exceptional plants, whether in living collections or cryo‐collections, requires more resources than the conservation of other species. Because of their expertise with rare plants, botanical gardens are well positioned to lead this effort, but a well‐developed strategy requires a clear understanding of the resources needed. Methods: Grant funding was obtained from the Institute of Museum and Library Services to support a three‐year project on cryobanking, and to provide smaller grants to 10 other botanical gardens for one‐year projects on either (1) seed behavior studies or (2) the development of protocols for in vitro propagation or cryopreservation. Results: Nine of the partner gardens worked on 19 species (one was unable to continue due to the COVID‐19 pandemic), while the larger project focused on 14 species. A point system was developed for tasks accomplished, and the average costs per point of the larger and smaller projects were similar. Labor accounted for half the costs. Projects focused on species in the Asteraceae and Orchidaceae had lower costs per point than other species. Discussion: Both large and small projects can contribute to a strategy for exceptional plant conservation for similar costs. Prioritizing species with lower costs could help advance the field while allowing time for work on more difficult species to develop. [ABSTRACT FROM AUTHOR]

The processing of ash/slag wastes were studied, while a complete analysis of the bottom ash of the Luchegorsk thermal power plant in the Primorsky Territory was carried out, and a study was performed on the separation of rare earth elements by various methods. The ash was previously cleaned from unburned coal (underburnt) and iron oxides, the resulting product was processed with various methods. Isolation and separation of products was carried out in several stages. The acidity of the medium was determined, which corresponds to the quantitative precipitation of iron and aluminum matrix ions depending on the initial concentrations of the solutions. Silicon oxide of 97% purity with a high specific surface area was obtained, as well as a solid product with a content of rare earth elements more than 100 times higher than in the original ash samples. The content of rare earth elements in the final product is 74.5% of the content of these elements in the original material, and the product is also enriched with other trace elements such as zirconium, hafnium, and tungsten. When the ash is processed, solid waste remains, which can be further utilized to fabricate ceramic materials and membranes with high technical performance. [ABSTRACT FROM AUTHOR]

Summary: Sapria himalayana Griff. (Rafflesiaceae) and its biology are described. Its distribution in Bhutan is mapped, taking into account recent records. [ABSTRACT FROM AUTHOR]

A rare plant species of the Orchidaceae family, Dendrobium officinale is considered among the top ten Chinese medicinal herbs for its polysaccharide. Since 2021, when the dieback disease of D. officinale was first reported in Yueqing City, Zhejiang Province, China, Fusarium isolates (number = 152) were obtained from 70 plants in commercial greenhouses. The disease incidence ranged from 40% to 60% in the surveyed areas. Multilocus sequence analysis (MLSA) coupled with morphological characterization revealed that the collected isolates belonged to five species (sp.), viz., Fusarium concentricum, F. fujikuroi, F. nirenbergiae, F. curvatum, and F. stilboides, with isolation frequencies of 34.6%, 22.3%, 18.4%, 13.8%, and 10.5%, respectively. Notably, at least two Fusarium species were simultaneously isolated and identified from the infected plants. Finally, the pathogenicity test results demonstrated that such species were responsible for the dieback disease of D. officinale. However, F. concentricum and F. fujikuroi were more invasive compared to the other species in this study. To the best of the authors' knowledge, this study was the first report of F. concentricum, F. curvatum, F. fujikuroi, F. nirenbergiae, and F. stilboides causing the dieback disease of D. officinale in China and worldwide. This work provides valuable data about the diversity and pathogenicity of Fusarium populations, which will help in formulating effective strategies and policies for better control of the dieback disease. [ABSTRACT FROM AUTHOR]

The ability of land plants to partially close their stomata in response to high vapor pressure deficit (VPD), called the limited transpiration trait, is a rare phenomenon in plants. The characteristic has been demonstrated in several crop species, including Sorghum bicolor. Genotypes within the sorghum species respond in different ways to high VPD, but the molecular basis and regulatory mechanisms of the responses are not clear. This study was conducted to determine the basis for the differences in stomatal responses to high VPD in sorghum genotypes (SC1345, SC35, and Macia). Gene expression patterns of genes involved in the abscisic acid pathway were investigated using quantitative-PCR after exposing plants to high VPD. The results showed marked differences in expression patterns for the genes across the three genotypes, coupled to differences in abscisic acid content. The results suggested natural variation in abscisic acid content within sorghum. Expression patterns in the genes BG1, ZEP, and SDR, in addition to the NCED3 gene, provide a basis for the variations in stomatal responses to high VPD in this crop species. [ABSTRACT FROM AUTHOR]

Epimedium elatum (Morren & Decne) belonging to the family Berberidaceae is a rare medicinal plant endemic to the Western Himalayas and is being rare species, it is an ideal case for DNA barcoding. The present study was aimed at molecular identification, authentication, and development of reference DNA barcodes for Epimedium elatum collected from the Western Himalayas of Pakistan. We investigated four reference DNA barcode markers namely, rbcL, matK, trnH-psbA, and ITS for molecular analysis of Epimedium elatum. Three out of the four DNA barcode markers showed 100% amplification and sequencing success rate; matK was the only exception. The nucleotide sequence data were analyzed using Sequencher, BLAST, Muscle alignment, BioEdit, and MEGA6 software. The ITS and TrnH-psbA sequences showed significant similarity to a related species of the genus Epimedium, while rbcL showed low discriminatory power. Further analysis revealed that all barcodes could clearly distinguish Epimedium elatum from its closely related species. We concluded that all three barcodes are powerful tools for the identification of Epimedium elatum. [ABSTRACT FROM AUTHOR]

Grasslands restored on arable land often retain high residual nutrients, modified soil biota, and lower plant species diversity. Establishment of rare plant species with complex multitrophic interactions, typical of undisturbed nutrient-poor environments, may be hindered by the absence of interacting organisms. We hypothesised that the addition of a mycorrhizal symbiont improves the seed germination of orchids that crucially depend on fungi. We focused on grasslands restored on arable land 1–15 years ago featuring residual mineral nutrients and low organic matter contents compared to semi-natural grasslands and on four orchid species differing in the level of mycorrhizal specificity: high – Anacamptis pyramidalis and Orchis mascula – and low – Platanthera bifolia and Gymnadenia conopsea. Five fungal isolates obtained from non-green underground mycorrhizal orchid seedlings (protocorms) or adults' roots were tested for orchid-fungus compatibility under conditions in vitro. Orchid seeds inserted in retrievable seed packets were subsequently co-introduced with selected fungal isolates grown either on agar or sterilized hay into the soil of nine restored grasslands and incubated for twelve months. The identity of mycorrhizal fungi in retrieved protocorms was verified by molecular methods. The isolates that supported protocorm establishment in vitro enabled also protocorm formation in situ , but success rates differed among orchid species. While mycorrhizal specialists produced most protocorms after inoculation, the mycorrhizal generalists took advantage of naturally occurring fungi and produced some protocorms both in inoculated and uninoculated treatments. We showed that the addition of mycorrhizal fungi enhanced protocorm formation regardless of the modified soil environment, especially in mycorrhizal specialist orchids. This method may help to restore populations of native orchid species in their former distribution ranges, including farming-altered habitats. [ABSTRACT FROM AUTHOR]

Background. Low-temperatures have the potential to be a serious problem for plants and can negatively affect the normal growth and development of walnuts. DREB1/CBF (Dehydration Responsive Element Binding Protein 1/C-repeat Binding Factor), one of the most direct transcription factors in response to low-temperature stress, may improve the resistance of plants to low-temperatures by regulating their functional genes. However, few studies have been conducted in walnut. The Xinjiang wild walnut is a rare wild plant found in China, with a large number of excellent trait genes, and is hardier than cultivated walnuts in Xinjiang. Methods. In this work, we identified all of the DREB1 members from the walnut genome and analyzed their expression levels in different tissues and during low-temperature stress on the Xinjiang wild walnut. The JfDREB1A gene of the Xinjiang wild walnut was cloned and transformed into Arabidopsis thaliana for functional verification. Results. There were five DREB1 transcription factors in the walnut genome. Among them, the relative expression level of the DREB1A gene was significantly higher than other members in the different tissues (root, stem, leaf) and was immediately un-regulated under low-temperature stress. The overexpression of the JfDREB1A gene increased the survival rates of transgenic Arabidopsis lines, mainly through maintaining the stability of cell membrane, decreasing the electrical conductivity and increasing the activities of antioxidant enzymes including superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT). Additionally, the expression levels of cold-inducible genes like AtKIN1, AtERD10, AtRD29A, AtCOR15A and AtCOR47, were significantly increased. These results showed that the JfDREB1A gene may play an important role in the response to cold stress of the Xinjiang wild walnut. This study contributes to our understanding of the molecular mechanism of the Xinjiang wild walnut's response to low-temperature stress and will be beneficial for developing walnut cultivars with improved cold resistance. [ABSTRACT FROM AUTHOR]