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1,701 results on '"Reinhardt Richard"'

153. A global view of the nonprotein-coding transcriptome in Plasmodium falciparum

Author

Raabe, Carsten A., Sanchez, Cecilia P., Randau, Gerrit, Robeck, Thomas, Skryabin, Boris V., Chinni, Suresh V., Kube, Michael, Reinhardt, Richard, Ng, Guey Hooi, Manickam, Ravichandran, Kuryshev, Vladimir Y., Lanzer, Michael, Brosius, Juergen, Tang, Thean Hock, and Rozhdestvensky, Timofey S.

Published
2010

154. H3K14ac is linked to methylation of H3K9 by the triple Tudor domain of SETDB1

Author

Jurkowska, Renata Z., Qin, Su, Kungulovski, Goran, Tempel, Wolfram, Liu, Yanli, Bashtrykov, Pavel, Stiefelmaier, Judith, Jurkowski, Tomasz P., Kudithipudi, Srikanth, Weirich, Sara, Tamas, Raluca, Wu, Hong, Dombrovski, Ludmila, Loppnau, Peter, Reinhardt, Richard, Min, Jinrong, and Jeltsch, Albert

Subjects
Binding Sites, Tudor Domain, Science, Acetylation, Mouse Embryonic Stem Cells, Histone-Lysine N-Methyltransferase, Crystallography, X-Ray, Methylation, Article, Recombinant Proteins, Histones, Mice, HEK293 Cells, Long Interspersed Nucleotide Elements, Animals, Humans, lcsh:Q, Protein Methyltransferases, lcsh:Science, Protein Processing, Post-Translational, Protein Binding
Abstract

SETDB1 is an essential H3K9 methyltransferase involved in silencing of retroviruses and gene regulation. We show here that its triple Tudor domain (3TD) specifically binds to doubly modified histone H3 containing K14 acetylation and K9 methylation. Crystal structures of 3TD in complex with H3K14ac/K9me peptides reveal that peptide binding and K14ac recognition occurs at the interface between Tudor domains (TD) TD2 and TD3. Structural and biochemical data demonstrate a pocket switch mechanism in histone code reading, because K9me1 or K9me2 is preferentially recognized by the aromatic cage of TD3, while K9me3 selectively binds to TD2. Mutations in the K14ac/K9me binding sites change the sub-nuclear localization of 3TD. ChIP-seq analyses show that SETDB1 is enriched at H3K9me3 regions and K9me3/K14ac is enriched at SETDB1 binding sites overlapping with LINE elements, suggesting that recruitment of the SETDB1 complex to K14ac/K9me regions has a role in silencing of active genomic regions., SETDB1 is a histone methyltransferase that generates H3K9me3 marks in euchromatic regions. Here the authors show that the triple Tudor domain (3TD) of SETDB1 binds histone H3 tails containing K14 acetylation combined with K9 methylation, and that the K9me–K14ac modification defines a novel chromatin state enriched at SETDB1 binding sites.

Published
2017

155. Effect of an aldose reductase inhibitor on alveolar bone loss associated with periodontitis in diabetic rats

Author

Kador, Peter F., Hamada, Tomofumi, Reinhardt, Richard A., and Blessing, Karen

Subjects
Periodontitis -- Development and progression, Periodontitis -- Care and treatment, Periodontitis -- Models, Periodontitis -- Research, Aldose reductase -- Physiological aspects, Aldose reductase -- Research, Alveolar process -- Physiological aspects, Alveolar process -- Research, Enzyme inhibitors -- Dosage and administration, Enzyme inhibitors -- Research, Diabetes -- Complications and side effects, Diabetes -- Research, Health
Published
2010

166. Efficient targeted DNA methylation with chimeric dCas9-Dnmt3a-Dnmt3L methyltransferase

Author

Stepper, Peter, Kungulovski, Goran, Jurkowska, Renata Z, Chandra, Tamir, Krueger, Felix, Reinhardt, Richard, Reik, Wolf, Jeltsch, Albert, Jurkowski, Tomasz P, Reik, Wolf [0000-0003-0216-9881], and Apollo - University of Cambridge Repository

Subjects
Epigenomics, Recombinant Fusion Proteins, High-Throughput Nucleotide Sequencing, DNA, DNA Methylation, Endonucleases, Models, Biological, Epigenesis, Genetic, Mice, Gene Expression Regulation, Cell Line, Tumor, Animals, Cluster Analysis, Humans, CpG Islands, DNA (Cytosine-5-)-Methyltransferases, Promoter Regions, Genetic
Abstract

DNA methylation plays a critical role in the regulation and maintenance of cell-type specific transcriptional programs. Targeted epigenome editing is an emerging technology to specifically regulate cellular gene expression in order to modulate cell phenotypes or dissect the epigenetic mechanisms involved in their control. In this work, we employed a DNA methyltransferase Dnmt3a-Dnmt3L construct fused to the nuclease-inactivated dCas9 programmable targeting domain to introduce DNA methylation into the human genome specifically at the EpCAM, CXCR4 and TFRC gene promoters. We show that targeting of these loci with single gRNAs leads to efficient and widespread methylation of the promoters. Multiplexing of several guide RNAs does not increase the efficiency of methylation. Peaks of targeted methylation were observed around 25 bp upstream and 40 bp downstream of the PAM site, while 20-30 bp of the binding site itself are protected against methylation. Potent methylation is dependent on the multimerization of Dnmt3a/Dnmt3L complexes on the DNA. Furthermore, the introduced methylation causes transcriptional repression of the targeted genes. These new programmable epigenetic editors allow unprecedented control of the DNA methylation status in cells and will lead to further advances in the understanding of epigenetic signaling.

Published
2019
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172. DNAH5 Mutations Are a Common Cause of Primary Ciliary Dyskinesia with Outer Dynein Arm Defects

Author

Hornef, Nada, Olbrich, Heike, Horvath, Judit, Zariwala, Maimoona A., Fliegauf, Manfred, Loges, Niki Tomas, Wildhaber, Johannes, Noone, Peadar G., Kennedy, Marcus, Antonarakis, Stylianos E., Blouin, Jean-Louis, Bartoloni, Lucia, Nüsslein, Thomas, Ahrens, Peter, Griese, Matthias, Kuhl, Heiner, Sudbrak, Ralf, Knowles, Michael R., Reinhardt, Richard, and Omran, Heymut

Published
2006
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180. γδ T cells shape memory-phenotype αβ T cell populations in non-immunized mice

Author

Phalke, Swati Popat, Huang, Yafei, Rubtsova, Kira, Getahun, Andrew, Sun, Deming, Reinhardt, Richard Lee, O’Brien, Rebecca R.L., Born, Willi W.K., Phalke, Swati Popat, Huang, Yafei, Rubtsova, Kira, Getahun, Andrew, Sun, Deming, Reinhardt, Richard Lee, O’Brien, Rebecca R.L., and Born, Willi W.K.

Abstract

Size and composition of γδ T cell populations change dramatically with tissue location, during development, and in disease. Given the functional differentiation of γδ T cell subsets, such shifts might alter the impact of γδ T cells on the immune system. To test this concept, and to determine if γδ T cells can affect other immune cells prior to an immune response, we examined non-immunized mice derived from strains with different genetically induced deficiencies in γδ T cells, for secondary changes in their immune system. We previously saw extensive changes in pre-immune antibodies and B cell populations. Here, we report effects on αβ T cells. Similarly to the B cells, αβ T cells evidently experience the influence of γδ T cells at late stages of their pre-immune differentiation, as single-positive heat stable antigen-low thymocytes. Changes in these and in mature αβ T cells were most prominent with memory-phenotype cells, including both CD8+ and CD4+ populations. As previously observed with B cells, most of the effects on αβ T cells were dependent on IL-4. Unexpectedly, IL-4 seemed to be produced mainly by αβ T cells in the non-immunized mice, albeit strongly regulated by γδ T cells. Similarly to our findings with B cells, changes of αβ T cells were less pronounced in mice lacking all γδ T cells than in mice lacking only some, suggesting that the composition of the γδ T cell population determines the nature of the γδ-influence on the other pre-immune lymphocytes., SCOPUS: ar.j, info:eu-repo/semantics/published

Published
2019

182. temperature-regulated DEAD-box RNA helicase CrhR interactome: autoregulation and photosynthesis-related transcripts.

Author

Migur, Anzhela, Heyl, Florian, Fuss, Janina, Srikumar, Afshan, Huettel, Bruno, Steglich, Claudia, Prakash, Jogadhenu S S, Reinhardt, Richard, Backofen, Rolf, Owttrim, George W, and Hess, Wolfgang R

Subjects
RNA helicase, DNA helicases, GENETIC regulation, DRUG target, HELICASES, ELECTRON transport
Abstract

RNA helicases play crucial roles in RNA biology. In plants, RNA helicases are encoded by large gene families, performing roles in abiotic stress responses, development, the post-transcriptional regulation of gene expression, as well as housekeeping functions. Several of these RNA helicases are targeted to the organelles, the mitochondria and chloroplasts. Cyanobacteria are the direct evolutionary ancestors of plant chloroplasts. The cyanobacterium Synechocystis 6803 encodes a single DEAD-box RNA helicase, CrhR, that is induced by a range of abiotic stresses, including low temperature. Though the Δ crhR mutant exhibits a severe cold-sensitive phenotype, the physiological function(s) performed by CrhR have not been described. To identify transcripts interacting with CrhR, we performed RNA co-immunoprecipitation with extracts from a Synechocystis crhR deletion mutant expressing the FLAG-tagged native CrhR or a K57A mutated version with an anticipated enhanced RNA binding. The composition of the interactome was strikingly biased towards photosynthesis-associated and redox-controlled transcripts. A transcript highly enriched in all experiments was the crhR mRNA, suggesting an autoregulatory molecular mechanism. The identified interactome explains the described physiological role of CrhR in response to the redox poise of the photosynthetic electron transport chain and characterizes CrhR as an enzyme with a diverse range of transcripts as molecular targets. [ABSTRACT FROM AUTHOR]

Published
2021
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184. Nevada: a phony showdown with real guns

Author

Reinhardt, Richard

Subjects
Public lands -- Nevada, Wise use movement -- Nevada, Nevada -- Environmental policy
Abstract

Tonopah, Nye County, Nevada: Out here in the sun-blistered, wind-blasted, sage-and-silver spaces of southwestern Nevada, discussions of national politics are less frequently heard than a discouraging word. That dusty red […]

Published
1995

189. Desert storm

Author

Reinhardt, Richard

Subjects
Deserts -- Oregon, Wilderness areas -- Oregon
Published
1992

193. Pathogen Evasion of Chemokine Response Through Suppression of CXCL10

Author

Antonia, Alejandro L., primary, Gibbs, Kyle D., additional, Trahair, Esme D., additional, Pittman, Kelly J., additional, Martin, Amelia T., additional, Schott, Benjamin H., additional, Smith, Jeffrey S., additional, Rajagopal, Sudarshan, additional, Thompson, J. Will, additional, Reinhardt, Richard Lee, additional, and Ko, Dennis C., additional

Published
2019
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196. The Systems Architecture of Molecular Memory in Poplar after Abiotic Stress

Author

Georgii, Elisabeth, primary, Kugler, Karl, additional, Pfeifer, Matthias, additional, Vanzo, Elisa, additional, Block, Katja, additional, Domagalska, Malgorzata A., additional, Jud, Werner, additional, AbdElgawad, Hamada, additional, Asard, Han, additional, Reinhardt, Richard, additional, Hansel, Armin, additional, Spannagl, Manuel, additional, Schäffner, Anton R., additional, Palme, Klaus, additional, Mayer, Klaus F.X., additional, and Schnitzler, Jörg-Peter, additional

Published
2019
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