Search

We describe the crystal structure determination of the ARM domain of Drosophila SARM1 (dSARM1ARM), which required combination of a number of sources of phase information in order to obtain interpretable electron density maps. SARM1 is a central executioner of the process of axon degeneration, a common feature of the early phase of a range of neurodegenerative diseases. SARM1 is held in the inactive state in healthy axons by its N-terminal auto-inhibitory ARM domain, and is activated to cleave NAD+ upon injury, triggering the subsequent axon degeneration. To characterize the molecular mechanism of SARM1 activation, we sought to determine the crystal structure of the SARM1 ARM domain. Here we describe the recombinant production and crystallization of dSARM1ARM, as well as unconventional process used for structure determination. Crystals were obtained in the presence of NMN, a precursor of NAD+ and a potential activator of SARM1, only after in situ proteolysis of the N-terminal 63 residues. After molecular replacement attempts failed, we determined the crystal structure of dSARM1ARM at 1.65 Å resolution using the MIRAS phasing technique with the program autoSHARP, combining data from the native, SeMet-labelled, and Br-soaked crystals. The structure will further our understanding of the regulation of SARM1.

The asymmetric assembly and spontaneous resolution of chiral heterometal-organic frameworks from achiral precursors remain a great challenge. Herein, we report the formation and spontaneous resolution of three-dimensional (3D) chiral 3d–4f heterometal-organic frameworks from achiral ligands in the presence of lactic acid enantiomers as chiral inducers. In the absence of a chiral inducer, the reactants randomly generated both enantiopure crystals in equal measure. However, enantiopure lactic acid induced asymmetric resolution to achieve single chiral crystallization. DFT theoretical calculations showed that the absolute chiral resolution of the enantiomers is due to the difference in the interactions between the two enantiomeric Λ/Δ-[FeL3]3− units and the chiral inducer.

The Japanese Lifespan Study (LSS) of the A-Bomb survivors is the principal basis of the current legal radiological framework. Evidence provided for the first time here shows that internal exposure to radiologically significant quantities of Uranium-234 contained in sub-micron particle rainout from the un-fissioned weapon warhead, the Black Rain, is a missing exposure in the LSS analysis. It is argued that this is responsible for a background excess cancer risk in all the LSS dose groups. This, together with epidemiological evidence based on unexposed controls falsifies the LSS cancer vs. dose regression coefficients for internal exposure.

Forster resonance energy transfer (FRET) microscopy is an important tool suitable for studying molecular interactions in living cells. Optical section structured illumination microscopy (OS-SIM), like confocal microscopy, has about 200 nm spatial resolution. In this report, we performed quantitative 3-cube FRET imaging in OS-SIM mode and widefield microscopy (WF) mode, respectively, for living cells expressing FRET constructs consisting of Cerulean (C, donor) and Venus (V, acceptor). OS-SIM images exhibited higher resolution than WF images. Four spectral crosstalk coefficients measured under OS-SIM mode are consistent with those measured under WF mode. Similarly, the system calibration factors G and k measured under OS-SIM mode were consistent with those measured under WF mode. The measured FRET efficiency (E) values of C32V and C17V as well as C5V constructs, standard FRET plasmids, in living Hela cells were EC32VOSF=0.32±0.02,EC17VOSF=0.38±0.02 , and EC5VOSF=0.45±0.03 , and the measured acceptor-to-donor concentration ratios ( Rc ) were RC32VOSF=1.07±0.03 , RC17VOSF=1.09±0.03 , and RC5VOSF=1.02±0.04 , consistent with the reported values. Collectively, our data demonstrates that OS-SIM can be integrated into FRET microscopy to build an OS-SIM-FRET with confocal microscopy-like resolution.

Confocal imaging has shown that CELLULOSE SYNTHASE (CESA) particles move through the plasma membrane as they synthesize cellulose. However, the resolution limit of confocal microscopy circumscribes what can be discovered about these tiny biosynthetic machines. Here, we applied Structured Illumination Microscopy (SIM), which improves resolution two-fold over confocal or widefield imaging, to explore the dynamic behaviors of CESA particles in living plant cells. SIM imaging reveals that Arabidopsis thaliana CESA particles are more than twice as dense in the plasma membrane as previously estimated, helping explain the dense arrangement of cellulose observed in new wall layers. CESA particles tracked by SIM display minimal variation in velocity, suggesting coordinated control of CESA catalytic activity within single complexes and that CESA complexes might move steadily in tandem to generate larger cellulose fibrils or bundles. SIM data also reveal that CESA particles vary in their overlaps with microtubule tracks and can complete U-turns without changing speed. CESA track patterns can vary widely between neighboring cells of similar shape, implying that cellulose patterning is not the sole determinant of cellular growth anisotropy. Together, these findings highlight SIM as a powerful tool to advance CESA imaging beyond the resolution limit of conventional light microscopy.

Surface-enhanced Raman spectroscopy (SERS), as induced by noble metal nanoparticles, is used for medical applications. In this study, gold nanoparticle parameters such as size, shape, separation, substrate etc. are varied systematically using electron beam lithographic fabrication methods. The resultant Raman spectra intensities are correlated with the nanoparticle surface plasmon energies as determined by electron energy loss spectroscopy (EELS) in a scanning transmission electron microscope (STEM) at nanometer-scale resolution. It is found that the largest enhancement is achieved when the illuminating laser energy closely matches, or is slightly higher than, the surface plasmon energies. In general, nanoparticle size is a strong determinant factor, and higher intensities are generated by sharply defined features. This approach allows a procedure to identify the optimum combination of nanoparticle parameters and laser energy to generate the largest Raman signals, thus enabling detection, for instance, of smaller, early-stage cancer tumors.

The article presents a new algorithm for increasing the resolution of thermal images. For this purpose, the residual network was integrated with the Kernel-Sharing Atrous Convolution (KSAC) image sub-sampling module. A significant reduction in the algorithm’s complexity and shortening the execution time while maintaining high accuracy were achieved. The neural network has been implemented in the PyTorch environment. The results of the proposed new method of increasing the resolution of thermal images with sizes 32 × 24, 160 × 120 and 640 × 480 for scales up to 6 are presented.

We report two- and three-dimensional (2D and 3D) 13C-17O heteronuclear correlation solid-state NMR experiments under magic-angle spinning (MAS) conditions. These experiments utilize the D-RINEPT (Dipolar-mediated Refocused Insensitive Nuclei Enhanced by Polarization Transfer) scheme with symmetry-based SR412 recoupling blocks for coherence transfer between 13C and 17O nuclei. First, a 2D 17O → 13C correlation experiment was performed for the [1-13C,17O]-Gly/Gly·HCl cocrystal and [U-13C, 1-17O]-α/β-d-glucose samples. Second, a 2D 17O → 13C MQ-D-RINEPT correlation experiment where the indirect dimension incorporates the multiple-quantum MAS (MQMAS) scheme was tested for obtaining isotropic 17O resolution with [U-13C, 1-17O]-α/β-d-glucose. Third, a new 3D 17O → 13C → 13C correlation experiment was demonstrated where 17O → 13C and 13C → 13C correlations are achieved by D-RINEPT and DARR (Dipolar Assisted Rotational Resonance) sequences, respectively (thus termed as a 3D D-RINEPT/DARR OCC experiment). This new 3D 17O NMR experiment is implemented with the aim for site-resolved solid-state 17O NMR studies.

Spatial resolution is an important criterion to evaluate the performance of a scintillation screen for X-ray imaging. Perovskite-based X-ray screen, usually made of powders or polycrystalline films, suffers from low spatial resolution (∼ 200 µm) due to the large thickness of scintillation layer despite of their compelling sensitivity to X-ray dose. In this work, a concentrated colloid of CsPbBr3 nanosheets was synthesized via a co-precipitation method at ambient condition. By drop casting, smooth scintillation screens of varied thickness were formed through self-assembly, which exhibited both high internal and external photoluminescence quantum yield (PL QY) (84.5% and 75.1%, respectively). The screen-based X-ray detector showed a high sensitivity down to 27 nGy/s, two orders of magnitude lower than the regular dose for medical diagnostics. Importantly, the screen of optimal thickness of 15 µm showcased an unprecedented spatial resolution (26 µm) when used for X-ray radiography, representing one order of magnitude improvement in perovskite community.

Many chiroptical spectroscopic techniques have been developed to detect chirality in molecular species and probe its role in biological processes. Raman optical activity (ROA) should be one of the most powerful methods, as ROA yields vibrational and chirality information simultaneously and can measure analytes in aqueous and biologically relevant solvents. However, despite its promise, the use of ROA has been limited, largely due to challenges in instrumentation. Here, we report a new approach to ROA that exploits high-frequency polarization modulation. High-frequency polarization modulation, usually implemented with a photoelastic modulator (PEM), has long been the standard technique in other chiroptical spectroscopies. Unfortunately, the need for simultaneous spectral and polarization resolution has precluded the use of PEMs in ROA instruments. We combine a specialized camera system (the Zurich Imaging Polarimeter, or ZIMPOL) with PEM modulation to perform ROA measurements. We demonstrate performance similar to the current standard in ROA instrumentation while reducing complexity and polarization artifacts. This development should aid researchers in exploiting the full potential of ROA for chemical and biological analysis.

Electron microscopy has enabled atomic resolution imaging of matter. However, unlike optical spectroscopic imaging, traditional electron microscopes provide limited spectroscopic information in terms of their energy resolution. Only recently, owing to advances in monochromated STEM-EELS, have transmission electron microscopes (TEMs) been able to attain a high energy resolution. We recently proposed combining spectrally selective photoexcitation with HRTEM to achieve sub-nanometer scale optical imaging, a technique we called photoabsorption microscopy using electron analysis (PAMELA). To realize PAMELA-TEM experimentally, we constructed a TEM holder with an optical feedthrough, capable of photoexciting materials with different wavelengths. In this article, we describe our process for designing and fabricating an optical TEM specimen holder, highlighting important aspects of the design.

Sound stimuli with rippled spectra are usable for testing the frequency resolving power of hearing. Estimates of the resolution depend on the center frequency and bandwidth of the test stimulus. In the present study, ripple density resolution was measured at various center frequencies and various bandwidths in two experimental paradigms: with the use of rippled or flat reference signals. With rippled reference signals, there was no statistically significant resolution dependence on either the center frequency or bandwidth. In all cases, the resolution was near 10 ripples/oct. With flat (nonrippled) reference signals, the ripple density resolution depended on both the center frequency and bandwidth: the higher the center frequency and the wider the frequency band, the higher the resolution was. The difference between resolution estimates obtained with different reference signals may be explained by different contributions of the spectral and temporal mechanisms of frequency analysis. The spectral (excitation pattern) mechanism is effective for discriminating between a rippled test and a rippled reference signal. The temporal processing mechanism is effective for discriminating between a rippled test and a flat reference signal.

Cryo-electron microscopy using single particle analysis requires the computational averaging of thousands of projection images captured from identical macromolecules. However, macromolecules usually present some degree of flexibility showing different conformations. Computational approaches are then required to classify heterogeneous single particle images into homogeneous sets corresponding to different structural states. Nonetheless, sometimes the attainable resolution of reconstructions obtained from these smaller homogeneous sets is compromised because of reduced number of particles or lack of images at certain macromolecular orientations. In these situations, the current solution to improve map resolution is returning to the electron microscope and collect more data. In this work, we present a fast approach to partially overcome this limitation for heterogeneous data sets. Our method is based on deforming and then moving particles between different conformations using an optical flow approach. Particles are then merged into a unique conformation obtaining reconstructions with improved resolution, contrast and signal-to-noise ratio. We present experimental results that show clear improvements in the quality of obtained 3D maps, however, there are also limits to this approach, i.e., the method is restricted to small deformations and cannot determine local patterns of flexibility of small elements, such as secondary structures, which we discuss in the manuscript.

The resolution performance of space charge measurement method determines its application environment and measured information, which has put forward higher temporal and spatial resolution requirements for the charge evaluation of electronic equipment insulation. Based on the ellipsometry detection principle and the photoelastic effect of the sensor, a novel optical method for space charge measurement is proposed. The potentials of the method in terms of single measurement with high signal-to-noise ratio, terahertz bandwidth and nanometer spatial resolution are analyzed. By deriving the mathematical relationship of the signal transmission, it is indicated that the light intensity difference measured by the system can linearly present the elastic force generated from space charge. Based on the built ellipsometry detection system, it is found that the detected signal from the sensor can quickly track the imposed elastic waves with different amplitudes and frequencies, which verifies the feasibility of the crucial detection part and the fast measurement speed of the proposed method. Further simulation results indicate that the refractivity performance of the sensor under the effect of elastic waves can present the deformation variation with a spatial resolution of 2 nm. Therefore, the given explorative research can provide the key theoretical basis and experimental verification for the proposed method.

We consider a linear-quadratic optimal control problem with indefinite matrices and the interval control constraint. The problem also has a regularizationparameter in the functional. The approximate solution of the problem is carried out on subsets of admissible controls, which are formed using linear combinations of special functions with an orientation to the optimal control structure due to the maximum principle. As a result of this procedure, a finite-dimensional quadratic optimization problem with the interval constraint on variables is obtained. The following relations between the variational problem and its finite-dimensional model are established: the convexity property of the optimal control problem is preserved for finite-dimensional model; a nonconvex optimal control problem under a certain condition on the regularization parameter (estimate from below) is approximated by a convex quadratic problem, which is solved in a finite number of operations;a special non-convex optimal control problem with an upper bound on the regularization parameter passes into the problem of minimizing a concave function on a finite set of points. A special case of a non-convex optimal control problem for the maximum of the norm of the final state is distinguished. Two procedures for improving the extreme points of finite-dimensional model are constructed, which reduce the computational costs for the global solution of the problem within the framework of the linearization method.

Despite the popularity of the analogue measuring devices, there is no recognized reference provided comprehensive guidelines for determining their resolution and its associated uncertainty. For filling this gap, this article is introduced as an attempt to explore and investigate all the available works related to this issue. The new terminology “evident space”, “evident resolution”, and “conjunction” were proposed to set a common reference. Two different techniques for determining the analogue resolution were presented and discussed. Several numerical examples were also presented to show different analogue indications and their interpretations. Moreover, reasonable recommendations for determining the resolution uncertainty were suggested depending on the case.

Understanding the lateral organization in plasma membranes remains an open problem and is of great interest to many researchers. Model membranes consisting of coexisting domains are commonly used as simplified models of plasma membranes. The coarse-grained (CG) Martini force field has successfully captured spontaneous separation of ternary membranes into a liquid-disordered and a liquid-ordered domain. With all-atom (AA) models, however, phase separation is much harder to achieve due to the slow underlying dynamics. To remedy this problem, here, we apply the virtual site (VS) hybrid method on a ternary membrane composed of saturated lipids, unsaturated lipids, and cholesterol to investigate the phase separation. The VS scheme couples the two membrane leaflets at CG and AA resolution. We found that the rapid phase separation reached by the CG leaflet can accelerate and guide this process in the AA leaflet.

A prototype of the electromagnetic calorimeter for the GAMMA-400 γ-ray telescope has been calibrated at the Pakhra S-25R electron synchrotron of the Lebedev Physical Institute. The measured energy resolution of the GAMMA-400 calorimeter is consistent with the results of the Monte Carlo simulation. The applicability of the Pakhra S-25R accelerator for calibrating detectors in various experiments has been confirmed.

Malaria, caused by parasites of the species Plasmodium, is among the major life-threatening diseases to afflict humanity. The infectious cycle of Plasmodium is very complex involving distinct life stages and transitions characterized by cellular and molecular alterations. Therefore, novel single-cell technologies are warranted to extract details pertinent to Plasmodium-host cell interactions and underpinning biological transformations. Herein, we tested two emerging spectroscopic approaches: (a) Optical Photothermal Infrared spectroscopy and (b) Atomic Force Microscopy combined with infrared spectroscopy in contrast to (c) Fourier Transform InfraRed microspectroscopy, to investigate Plasmodium-infected erythrocytes. Chemical spatial distributions of selected bands and spectra captured using the three modalities for major macromolecules together with advantages and limitations of each method is presented here. These results indicate that O-PTIR and AFM-IR techniques can be explored for extracting sub-micron resolution molecular signatures within heterogeneous and dynamic samples such as Plasmodium-infected human RBCs. Infrared imaging systems can characterise the spatial distribution of molecular features in cells, for example to aid in the study of malaria. Here three such techniques are systematically compared in their ability to characterise malaria-infected red blood cells.

A multichannel scintillation spectrometer with a thickness of 2.5X0 has been calibrated on a secondary electron beam with energies of 23−280 MeV at the Pakhra accelerator of the Lebedev Physical Institute. The relative energy resolution of the spectrometer is determined by the electron energy and the spectrometer thickness. The best relative energy resolution of the spectrometer is attained at an electron energy of ~90 MeV; its values are δ = 13 and 9.4% at spectrometer thicknesses of 1.0X0 and 2.5X0, respectively.

BackgroundMembrane potential (Vmem) exerts physiological influence across a wide range of time and space scales. To study Vmem in these diverse contexts, it is essential to accurately record absolute values of Vmem, rather than solely relative measurements.Materials & MethodsWe use fluorescence lifetime imaging of a small molecule voltage sensitive dye (VF2.1.Cl) to estimate mV values of absolute membrane potential.ResultsWe test the consistency of VF2.1.Cl lifetime measurements performed on different single photon counting instruments and find that they are in striking agreement (differences of mem under two-photon (2P) illumination with better than 20 mV of Vmem resolution, a nearly 10-fold improvement over other lifetime-based methods.ConclusionsWe demonstrate that VF-FLIM is a robust and portable metric for Vmem across imaging platforms and under both one-photon and two-photon illumination. This work is a critical foundation for application of VF-FLIM to record absolute membrane potential signals in thick tissue.

With the advancement of aberration correction techniques, transcranial ultrasound imaging has exhibited great potential in applications such as imaging neurological function and guiding therapeutic ultrasound. However, the feasibility of transcranial imaging varies among individuals because of the differences in skull acoustic properties. To better understand the fundamental mechanisms underlying the variation in imaging performance, the effect of the structure of the porous trabecular bone on transcranial imaging performance (i.e., target localization errors and resolution) was investigated for the first time through the use of elastic wave simulations and experiments. Simulation studies using high-resolution computed tomography data from ex vivo skull samples revealed that imaging at large incidence angles reduced the target localization error for skulls having low porosity; however, as skull porosity increased, large angles of incidence resulted in degradation of resolution and increased target localization errors. Experimental results indicate that imaging at normal incidence introduced a localization error of 1.85 ± 0.10 mm, while imaging at a large incidence angle (40°) resulted in an increased localization error of 6.54 ± 1.33 mm and caused a single point target to no longer appear as a single, coherent target in the resulting image, which is consistent with simulation results. This first investigation of the effects of skull microstructure on transcranial ultrasound imaging indicates that imaging performance is highly dependent on the porosity of the skull, particularly at non-normal angles of incidence.

This paper proposes an interrogation method to improve the resolution of DFB (distributed feedback) fiber laser acoustic emission (AE) sensing system. In this method, the coefficients of three ellipses formed by every two-channel interference fringe are estimated using bias-corrected ellipse-specific fitting (BCESF). The system parameters can be further corrected using the coefficients of the three ellipses. A resolution of $2\times 10 ^{-7}$ pm/ $\surd $ Hz at 100 kHz, which is approximately 10 dB higher than the traditional method, is achieved using the system parameters correction. The carbon fiber reinforced plastic (CFRP) laminates tensile test shows that the improved DFB fiber laser AE sensing system exhibits better performance than the PZT sensor. The damage initiation, damage spread, and instability stages of CFRP laminates’ failure process can be accurately recognized.

It is shown here that microwave sensors can be used to monitor glucose in serum concentration with minimum detectable as well as resolution of 1 mMol $\cdot \text{L}^{{-1}}$ ( $\approx 18$ mg $\,\cdot $ dL−1). The ultrasensitive detection technique relies on a split ring resonator, operating at the frequency of 1.156 GHz, as the core of the sensor where its loss is compensated to enhance the quality factor from $\sim 190$ (passive mode) to $\sim 3850$ (active mode) to enable high resolution (modified frequency detection error from ±12 kHz down to ±2.5 kHz) frequency-shift sensing. Initially, glucose concentrations of 100–1000 mMol $\,\cdot \text{L}^{{-1}}$ (1800–18000 mg $\,\cdot $ dL−1) in water were detected within 250 kHz of dynamic range (between two spectrum ends). Selectivity of the sensor to glucose is verified with respect to common interstitial fluid ingredients with biological levels. Finally, to enhance the resolution of the proposed sensor, its loss–compensation is further improved leading to increased accuracy of measuring glucose samples in a 0.9 % NaCl solution containing 10 % horse serum that closely resembles blood plasma and interstitial fluid. This allows exploration of lower concentrations in the physiological range 1–30 mMol $\,\cdot \text{L}^{{-1}}$ (18–540 mg $\,\cdot $ dL−1) with improved frequency detection error down to ±0.75 kHz for two cases of with/without serum solutions with dynamic range of 30 kHz/38 kHz. The highly accurate glucose monitoring technique could be utilized for developing noninvasive glucose sensors for biomedical applications in real–time glucose monitoring.

A retinal detachment is the term used to describe detachment of the neurosensory retina from the underlying membrane, the retinal pigment epithelium (RPE). Rhegmatogenous detachments are caused by a break in the retina through which fluid passes from the vitreous cavity into the subretinal space. The incidence of rhegmatogenous retinal detachment in the general population in Europe is 1 in 10 000 persons per year. Danger is the greatest in the age range from 55 to 70 years. Without treatment, blindness in the affected eye may occur. Individual risks depend on the presence or absence of specific factors including myopia, positive family anamnesis, retina rupture, trauma, ablation in the other eye, ablation in a vitreous body, retina high-risk peripheral de-generations and vitreoretinal degenerations. Majority of the untreated rhegmatogenous retina ablations progress to the subtotal or total retinal detachment and blindness. This paper describes a very rare case of the spontaneous complete reattachment of the sensory retina to the retinal pigment epithelium in a patient with the total rhegmatogenous retinal ablation in the right eye. The female patient, who was 52 years old, was examined by an Ophthalmologist after she had experienced a sudden loss of vision, 2 months before appointment. After a detailed ophthalmological examination, a total rhegmatogenous retinal ablation of the right eye was diagnosed. The best corrected visual acuity, evaluated on a Snellen chart, was 2/60. The patient was referred to a tertiary-level Institution since a surgical intervention of the ablation was needed. Due to technical inabilities in the above-mentioned Institution, the operation was not performed, and despite the recommendation to perform the intervention in another tertiary level Institution, the patient did not have ophthalmological examinations during the following three months. During the next visit, the Ophthalmologist determined that there was a spontaneous retinal fixation on the retinal pigment epithelium and a partial restoration of the visual function of the affected eye which was evaluated at 0.5.

The impact of Time-of-Flight (TOF) on positron emission tomography (PET) spatial resolution is generally considered negligible. In this work, a two-step approach based on simulations of two-dimensional scanner configurations is taken to show that ultra-fast TOF has the potential to overcome the limitation induced by the physical size of detectors on spatial resolution. An estimation of the lower bound on spatial resolution using point sources is provided, followed by a qualitative assessment of the resolution obtained using a Hot Spot phantom. The impact of detector width, TOF resolution and TOF binning on the achieved spatial resolution is also studied. While gain beyond the expected blur due to detector size is demonstrated, the detector size remains one limiting factor albeit less prominent. The dependence on acquisition statistics to reach the full potential of TOF-induced gain in spatial resolution is demonstrated. A simulated brain phantom acquired with a fictive three-dimensional PET scanner was qualitatively analyzed and structures smaller than the typical limit are clearly made visible by reconstructing the images with a ∼13-ps TOF resolution. A potential application of this feature of ultra-fast TOF would be the design of clinical PET scanners achieving spatial resolution beyond the current state-of-the-art.

In this paper, we discuss the resolution of max - M compositional and min - M compositional fuzzy relation equations over complete lattices , where M is a left-increasing or right-increasing binary operation . The solvability, the existence of the least and the greatest solutions, and the unique solvability of these fuzzy relation equations are characterized.

Quaternary Cd0.9Zn0.1Te1– y Se y (CZTS) single crystals, a novel room-temperature nuclear radiation detector semiconductor material, have been grown using a modified vertical Bridgman method (VBM) and a traveling heater method (THM). The percentage concentration of selenium in the VBM-grown crystal was 3% and that in the THM-grown crystal was 2%. While the THM Frisch collar detector ( $4.4\times 4.4\times10.7$ mm $^{3}$ ) produced a highly resolved pulse height spectra (PHS) with a resolution of ~1.06% for 662-keV gamma rays without any correction, the VBM-grown detector ( $10\times 10\times 2$ mm $^{3}$ ) offered a high-energy resolution of ~2% after the application of a digital bi-parametric correction. The high-resolution performance of these detectors has been attributed to the addition of Se in the Cd0.9Zn0.1Te (CZT) matrix. Ab-initio calculations based on density functional theory (DFT) also confirmed that the addition of Se in the CZT matrix helps to reduce the formation of TeCd and the TeZn antisites. The VBM-grown crystals were characterized using powder X-ray diffraction (XRD) and energy dispersive X-ray spectroscopy (EDS). While the XRD results revealed sharp diffraction peaks confirming the crystalline nature of the grown crystal, the EDS results confirmed the targeted stoichiometry of the elemental composition. The bulk resistivity of the grown crystal was calculated to be $\sim 3\times 10^{10}\,\,\Omega$ -cm from the current–voltage characteristics recorded at room temperature in a planar configuration, ensuring that the grown CZTS crystals have low dark current as required for detector-grade crystals.

Constructing low-dimensional covalent assemblies with tailored size and connectivity is challenging yet often key for applications in molecular electronics where optical and electronic properties of the quantum materials are highly structure dependent. We present a versatile approach for building such structures block by block on bilayer sodium chloride (NaCl) films on Cu(111) with the tip of an atomic force microscope, while tracking the structural changes with single-bond resolution. Covalent homo-dimers in cis and trans configurations and homo-/hetero-trimers were selectively synthesized by a sequence of dehalogenation, translational manipulation and intermolecular coupling of halogenated precursors. Further demonstrations of structural build-up include complex bonding motifs, like carbon–iodine–carbon bonds and fused carbon pentagons. This work paves the way for synthesizing elusive covalent nanoarchitectures, studying structural modifications and revealing pathways of intermolecular reactions., Tailoring the size and connectivity of organic nanostructures is challenging but is often key in molecular electronics for tuning the properties of the quantum materials. Now an approach has been developed for building low-dimensional covalent architectures block by block on a surface by highly selective tip-induced intermolecular reactions.

Understanding the neural correlates of risk-sensitive skin conductance responses can provide insights into their connection to emotional and cognitive processes. To provide insights into this connection, we studied the cortical correlates of risk-sensitive skin conductance peaks using electroencephalography. Fluctuations in skin conductance responses were elicited while participants played a threat-of-shock card game. Precise temporal information about skin conductance peaks was obtained by applying continuous decomposition analysis on raw electrodermal signals. Shortly preceding skin conductance peaks, we observed a decrease in oscillatory power in the frequency range between 3 and 17 Hz in occipitotemporal cortical areas. Atlas-based analysis indicated the left lingual gyrus as the source of the power decrease. The oscillatory power averaged across 3-17 Hz showed a significant negative relationship with the skin conductance peak amplitude. Our findings indicate a possible interaction between attention and threat perception.

To improve positron emission tomography (PET) image quality, depth-of-interaction (DOI) information and time-of-flight (TOF) information are key technologies. In this work, we developed the DOI-TOF detector based on our original single-ended readout scheme with the continuous layered gadolinium fine aluminum garnet (GFAG) array. The size of each GFAG crystal is $1.45\times 1.45\times 20$ mm3. The multipixel photon counter (MPPC) used as a photo sensor has a surface area of $3.0\times 3.0$ mm2. One pair of crystals coupled with a partial optical window is arranged across two MPPCs. Boundaries without the partial optical window are covered with optical reflectors. Each pair is coupled with paired MPPCs of different patterns. Crystal identification is obtained from paired MPPCs of different patterns and output rates. By limiting the spread of scintillation light, the proposed DOI detector based on the local centroid calculation was expected to improve crystal response, including the edge. For performance comparison, we prepared three discrete layers of the GFAG array with the same total thickness. For the continuous layer, the DOI resolution, the energy resolution and the timing resolution of the pair of detectors are 4.7 mm, 14%, and 402 ps, respectively. Their respective values are 29%, 20%, and 33% better than those with the three discrete layers.

Essential Cases: Public Law provides a bridge between course textbooks and key case judgments. This case document summarizes the facts and decision in Re Resolution to Amend the Constitution of Canada [1981] 1 SCR 753, also known as the Patriation Reference, Supreme Court of Canada. This case considers the identification of constitutional conventions using the Jennings test, and the legal enforceability of conventions. The document also includes supporting commentary and questions from author Thomas Webb.

Methods for the validation of 3D maps from cryo-EM are described and illustrated using an example map of bacteriophage P22. Methods for fitting, flexible fitting, refinement and annotation of models generated based on such maps are also explored., The process of turning 2D micrographs into 3D atomic models of the imaged macromolecules has been under rapid development and scrutiny in the field of cryo-EM. Here, some important methods for validation at several stages in this process are described. Firstly, how Fourier shell correlation of two independent maps and phase randomization beyond a certain frequency address the assessment of map resolution is reviewed. Techniques for local resolution estimation and map sharpening are also touched upon. The topic of validating models which are either built de novo or based on a known atomic structure fitted into a cryo-EM map is then approached. Map–model comparison using Q-scores and Fourier shell correlation plots is used to assure the agreement of the model with the observed map density. The importance of annotating the model with B factors to account for the resolvability of individual atoms in the map is illustrated. Finally, the timely topic of detecting and validating water molecules and metal ions in maps that have surpassed ∼2 Å resolution is described.

In this study, we investigate the response of tropical cyclones (TCs) to climate change by using the Princeton environment-dependent probabilistic tropical cyclone (PepC) model and a statistical-deterministic method to downscale TCs using environmental conditions obtained from the Geophysical Fluid Dynamics Laboratory (GFDL) High-resolution Forecast-oriented Low Ocean Resolution (HiFLOR) model, under the Representative Concentration Pathway 4.5 (RCP4.5) emissions scenario for the North Atlantic basin. The downscaled TCs for the historical climate (1986-2005) are compared with those in the mid- (2016-35) and late-twenty-first century (2081-2100). The downscaled TCs are also compared with TCs explicitly simulated in HiFLOR. We show that while significantly more storms are detected in HiFLOR towards the end of the twenty-first century, the statistical-deterministic model projects a moderate increase in TC frequency, and PepC projects almost no increase in TC frequency. The changes in storm frequency in all three datasets are not significant in the mid-twenty-first century. All three project that storms will become more intense and the fraction of major hurricanes and Category 5 storms will significantly increase in the future climates. However, HiFLOR projects the largest increase in intensity while PepC projects the least. The results indicate that HiFLOR’s TC projection is more sensitive to climate change effects and statistical models are less sensitive. Nevertheless, in all three datasets, storm intensification and frequency increase lead to relatively small changes in TC threat as measured by the return level of landfall intensity.

In a powder diffraction experiment the resolution function defines the instrumental contribution to the peak widths as a function of the Bragg angle. The Caglioti formula is frequently applied to model the instrumental broadening and used in structural refinement. The parameters in the Caglioti formula are linked to physically meaningful parameters for most diffraction geometries. However, this link is lost for the now very popular powder diffraction geometry using large 2D area detectors. Here we suggest a new physical model for the instrumental broadening specifically developed for powder diffraction data measured with large 2D area detectors. The model is verified using data from two synchrotron diffraction beamlines with the Pilatus2M and MAR345 detectors. Finally, a functional form is proposed to replace the Caglioti formula for this geometry in the Rietveld method and profile refinements.

SUMMARY The equivalent source technique is a powerful and widely used method for processing gravity and magnetic data. Nevertheless, its major drawback is the large computational cost in terms of processing time and computer memory. We present two techniques for reducing the computational cost of equivalent source processing: block-averaging source locations and the gradient-boosted equivalent source algorithm. Through block-averaging, we reduce the number of source coefficients that must be estimated while retaining the minimum desired resolution in the final processed data. With the gradient-boosting method, we estimate the sources coefficients in small batches along overlapping windows, allowing us to reduce the computer memory requirements arbitrarily to conform to the constraints of the available hardware. We show that the combination of block-averaging and gradient-boosted equivalent sources is capable of producing accurate interpolations through tests against synthetic data. Moreover, we demonstrate the feasibility of our method by gridding a gravity data set covering Australia with over 1.7 million observations using a modest personal computer.

This article examines principles of environmental law been applied by courts in their dispute resolution activities. The aim of the study is to systematize the applied in certain categories of cases principles. The tasks set by the author are to identify the correctness of the application, the correspondence of the meaning of the principle to which the court refers and the need set before the court – the dispute under consideration. Methods used in the article are method of analysis and synthesis that suits the goal. Environmental requirements and their corresponding principles are universal. The considered principles of environmental and land law are important in resolving legal disputes, playing the role of pillars of law, they are referenced in almost every court decision. Whether their indication in decisions is limited judicial lawmaking or elimination of a conflict, as well as the use of an analogy of law or law in the absence of an appropriate rule of law, is to be clarified in this study based on the analysis of judicial practice. Currently, there are a number of problems related to the application of the principles of environmental law, for example, the need to expand the list of principles of environmental law enshrined in legal norms with insufficient legislative support for the implementation. The environmental doctrine is developing actively nowadays; it provides a high probability of improving legal regulation in the field of environmental legal relations and the principles of environmental law in the future. Keywords: principles of environmental law, judicial practice in environmental disputes, application of the principles of environmental law by the courts, inconsistency of judicial practice with the essence of the principle

I develop a puzzle, the resolution of which, I argue, requires an unfamiliar distinction between two forms or senses of metaphysical modality, each bearing a different relationship to time. In one ...

When the conventional Hertz formula is used to extract the elastic modulus, E, of cells based on the compression test using atomic force microscope spherical probe, the inconsistency between the actual situation and the assumption of the formula will lead to a large error. Using the ABAQUS for finite element modeling and analysis, here, a modified Hertz formula was developed to reduce the effects of cell radius, cell thickness, probe radius and compression depth on the extracted E of cells. Experimentally, the insensitivity of the extracted E to the compression region of cell and probe radius reflects the validity of the modified formula. Owing to the poor resolution of spherical probes, it's unlikely to know the actual thickness of cell at the measured point, which can lead to a huge error. Based on the modified formula, we further proposed an approach to control the effect of the uncertainty of cell thickness and ensured that a 10% difference in cell thickness does not incur over 10% variation in the obtained elastic modulus.

A high resolution spatiotemporal ultrafast pump-probe system is developed to examine the interactions and transport phenomena between the electrical and the lattice thermal subsystems during ultrafast laser-matter interactions. This system incorporates an ultrafast pump-probe scheme with a stationary probe beam that interrogates the response to a spatial scanning pump beam, providing a full spatiotemporal mapping of a material's response due to an ultrafast pump excitation. The material's response, which is highly sensitive to its transport properties, is measured with a high spatial accuracy of up to ±10 nm and subpicosecond time resolution. Details of achieving this high spatial accuracy are described, and a study of the ultrafast transport processes in thin film gold is demonstrated. With the aid of transport and optical response models, the electrical thermal transport properties of gold and the electron-lattice coupling constant are simultaneously determined.

Visualization interfaces designed for heterogeneous devices such as wall displays and mobile screens must be responsive to varying display dimensions, resolution, and interaction capabilities. In this paper, we report on two user studies of visual representations for large versus small displays. The goal of our experiments was to investigate differences between a large vertical display and a mobile hand-held display in terms of the data comprehension and the quality of resulting insights. To this end, we developed a visual interface with a coordinated multiple view layout for the large display and two alternative designs of the same interface – a space-saving boundary visualization layout and an overview layout – for the mobile condition. The first experiment was a controlled laboratory study designed to evaluate the effect of display size on the perception of changes in a visual representation, and yielded significant correctness differences even while completion time remained similar. The second evaluation was a qualitative study in a practical setting and showed that participants were able to easily associate and work with the responsive visualizations. Based on the results, we conclude the paper by providing new guidelines for screen-responsive visualization interfaces.

In this paper, we present a new method for the resolution of a multi-level multi- objective linear programming problem with bounded variables based on the adaptive method for solving multi-level mu...